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Recent Articles  

21 pages, 3446 KiB  
Article
Targeting the Kynureninase–HDAC6–Complement Axis as a Novel Therapeutic Strategy in Glioblastoma
by Arif Ul Hasan, Sachiko Sato, Mami Obara, Yukiko Kondo and Eiichi Taira
Epigenomes 2025, 9(3), 27; https://doi.org/10.3390/epigenomes9030027 - 28 Jul 2025
Abstract
Background/Objectives: Glioblastoma (GBM) is an aggressive brain tumor known for its profound heterogeneity and treatment resistance. Dysregulated complement signaling and epigenetic alterations have been implicated in GBM progression. This study identifies kynureninase (KYNU), a key enzyme in the kynurenine pathway, as a novel [...] Read more.
Background/Objectives: Glioblastoma (GBM) is an aggressive brain tumor known for its profound heterogeneity and treatment resistance. Dysregulated complement signaling and epigenetic alterations have been implicated in GBM progression. This study identifies kynureninase (KYNU), a key enzyme in the kynurenine pathway, as a novel regulator of complement components and investigates its interaction with histone deacetylase 6 (HDAC6) in the context of therapeutic targeting. Methods: KYNU expression, and its association with complement signaling in GBM, were analyzed using publicly available datasets (TCGA, GTEx, HPA). Pathway enrichment was performed via LinkedOmics. In vitro studies in GBM cell lines (U87, U251, T98G) assessed the effects of KYNU silencing and treatment with an HDAC6 inhibitor (tubastatin) and a BET inhibitor (apabetalone) on gene expression and cell viability. Results: Bioinformatic analyses revealed significant overexpression of KYNU in GBM tissues compared to normal brain tissue. KYNU expression was positively associated with genes involved in complement and coagulation cascades. In vitro experiments demonstrated that KYNU silencing reduced the expression of C3, C3AR1, and C5AR1 and suppressed GBM cell viability. Treatment with tubastatin, while reducing viability, paradoxically upregulated complement genes, suggesting potential limitations in therapeutic efficacy. However, this effect was mitigated by KYNU knockdown. Combined treatment with apabetalone and tubastatin effectively suppressed KYNU expression and enhanced cytotoxicity, particularly in cells with high complement expression. Conclusions: Our findings establish the KYNU–HDAC6–complement axis as a critical regulatory pathway in GBM. Targeting KYNU-mediated complement activation through combined epigenetic approaches—such as HDAC6 and BET inhibition—represents a promising strategy to overcome complement-driven resistance in GBM therapy. Full article
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26 pages, 4081 KiB  
Article
Melatonin-Induced Modulation of Polyphenols and Glycolytic Pathways in Relation to Postharvest Quality of Blue Honeysuckle Fruits
by Jinli Qiao, Liangchuan Guo, Zhen Xiao, Junwei Huo, Xiaonan Sui, Fang Gao and Yan Zhang
Foods 2025, 14(15), 2646; https://doi.org/10.3390/foods14152646 - 28 Jul 2025
Abstract
The impact of exogenous melatonin treatment on the postharvest quality and storability of blue honeysuckle fruit was investigated. Fruits were immersed in melatonin solutions at concentrations of 0 (control), 0.01, 0.05, and 0.25 mM for 5 min and subsequently stored at –1 °C [...] Read more.
The impact of exogenous melatonin treatment on the postharvest quality and storability of blue honeysuckle fruit was investigated. Fruits were immersed in melatonin solutions at concentrations of 0 (control), 0.01, 0.05, and 0.25 mM for 5 min and subsequently stored at –1 °C for 63 d. Among all treatments, the combination of two-week storage without fruit puncturing and 0.05 mM melatonin application significantly delayed fruit softening and decay even at the initial stage of storage, while also increasing the concentration of phenolic compounds and enhancing antioxidant activity. During the later storage period (28–63 d), melatonin-treated fruits maintained higher levels of maltose, fructose, and sucrose, contributing to improved flavor retention. In contrast, both lower (0.01 mM) and higher (0.25 mM) concentrations were less effective or even detrimental to fruit quality. HPLC-ESI-QTOF-MS2 analysis revealed that 0.05 mM melatonin effectively preserved several functional phenolics, including p-coumaroylquinic acid, caffeoyl glucose, 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, luteolin-7-O-glucoside, and hydroxytyrosol. Thus, 0.05 mM melatonin is effective in delaying senescence and maintaining the postharvest quality of blue honeysuckle fruit. Full article
(This article belongs to the Special Issue Refined Processing Technology to Improve the Quality of Natural Products in Foods)
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17 pages, 1388 KiB  
Article
Constrained Adaptive Weighted Particle Swarm Optimization (C-AWPSO) Algorithm for Dipping Fault Parameter Inversion
by Shiquan Su, Juntao Liang, Chuang Xu, Feiyu Zhang and Hangtao Yu
Appl. Sci. 2025, 15(15), 8382; https://doi.org/10.3390/app15158382 - 28 Jul 2025
Abstract
To overcome the limitations of gravity inversion methods in fault inversion, this paper proposed a constrained adaptive weighted particle swarm optimization algorithm. Simulation experiments demonstrate that the proposed method exhibits stronger noise resistance compared to traditional optimization methods. In practical cases, the inversion [...] Read more.
To overcome the limitations of gravity inversion methods in fault inversion, this paper proposed a constrained adaptive weighted particle swarm optimization algorithm. Simulation experiments demonstrate that the proposed method exhibits stronger noise resistance compared to traditional optimization methods. In practical cases, the inversion accuracy of this method is improved by at least 64.4%, and the predicted gravity anomaly curve is closer to the observed data. The research findings are as follows: (1) The linearly decreasing inertia weight strategy performs best in terms of convergence efficiency and global search capability; (2) among the fault parameters, the top-layer center depth z and bottom-layer center depth w show higher sensitivity, and the inversion results for these parameters are more stable, which is beneficial for determining the depth information of faults; (3) introducing L2 regularization and penalty terms as constraints significantly improves the inversion stability, and among these, z and w have a particularly notable impact on the error. Full article
9 pages, 479 KiB  
Review
Photobiomodulation as a Hypothetical Strategy to Reverse Botulinum Toxin Effects: Exploring the Neuroregenerative Mechanisms and Translational Potential
by Rodrigo Álvaro Brandão Lopes-Martins, Francisco Gonzalez-Lima, Sérgio Gomes da Silva, Patrícia Sardinha Leonardo, Cristiane Soncino, Roberto Fernandes Pacheco, Carolina Lúcia de Oliveira e Oliveira and Fabrizio dos Santos Cardoso
Life 2025, 15(8), 1206; https://doi.org/10.3390/life15081206 - 28 Jul 2025
Abstract
Background: Botulinum toxin type A (BoNT/A) is widely used in both clinical and aesthetic settings to induce temporary neuromuscular paralysis by inhibiting acetylcholine release. Although generally regarded as safe and effective, complications such as iatrogenic ptosis or facial asymmetry may occur and persist [...] Read more.
Background: Botulinum toxin type A (BoNT/A) is widely used in both clinical and aesthetic settings to induce temporary neuromuscular paralysis by inhibiting acetylcholine release. Although generally regarded as safe and effective, complications such as iatrogenic ptosis or facial asymmetry may occur and persist for several weeks or even months, with no standardized method currently available to accelerate recovery. Objective: This article explores the hypothesis that photobiomodulation (PBM)—a non-invasive modality recognized for its neuroregenerative potential—may facilitate the reversal of BoNT/A-induced neuromuscular blockade. Discussion: PBM enhances mitochondrial activity by stimulating cytochrome c oxidase in nerve and muscle tissues, thereby increasing ATP production and modulating intracellular signaling pathways associated with neuroplasticity, cell survival, and synaptogenesis. Preclinical studies have demonstrated that PBM can upregulate neurotrophic factors (e.g., BDNF, NGF), enhance SNAP-25 expression, and promote structural remodeling of neurons in both young and aged brains. These mechanisms are biologically consistent with the regenerative processes required for recovery from BoNT/A-induced effects. While controlled clinical trials for this specific application are currently lacking, anecdotal clinical reports suggest that PBM may accelerate functional recovery in cases of BoNT/A-related complications. Conclusions: Although this approach has not yet been tested in clinical trials, we propose that photobiomodulation may hypothetically serve as a supportive strategy to promote neuromuscular recovery in patients experiencing adverse effects from BoNT/A. This hypothesis is grounded in robust preclinical evidence but requires validation through translational and clinical research. Full article
(This article belongs to the Section Physiology and Pathology)
22 pages, 1081 KiB  
Article
Antioxidant and Anti-inflammatory Activities of Latilactobacillus curvatus and L. sakei Isolated from Green Tripe
by Ga Hun Lee, Sung Hyun Choi, Yong Hyun Lee and Jae Kweon Park
Nutrients 2025, 17(15), 2464; https://doi.org/10.3390/nu17152464 - 28 Jul 2025
Abstract
Background/Objectives: Green tripe (GRET) is rich in essential fatty acids, vitamins, calcium, phosphorus, and other nutrients and contains various beneficial microorganisms, including lactobacillus, along with feed components consumed by ruminants. Methods: In this study, Latilactobacillus sakei and L. curvatus were isolated from GRET [...] Read more.
Background/Objectives: Green tripe (GRET) is rich in essential fatty acids, vitamins, calcium, phosphorus, and other nutrients and contains various beneficial microorganisms, including lactobacillus, along with feed components consumed by ruminants. Methods: In this study, Latilactobacillus sakei and L. curvatus were isolated from GRET and evaluated for their potential as probiotics, focusing on their anti-inflammatory properties and ability to modulate inflammatory responses. Results: When heat-killed L. sakei or L. curvatus (108 CFU/mL) and their metabolites (0.5 mg/mL) were applied to RAW 264.7 macrophages stimulated with LPS, nitric oxide (NO) production was reduced by approximately 10–35% and 2–11%, respectively. Furthermore, the expression levels of key anti-inflammatory cytokines, TNF-α and IL-6, were suppressed by more than 5%. These effects were not due to cytotoxicity but instead due to genuine anti-inflammatory activity. In addition, both strains exhibited antioxidant activity, as demonstrated by their performance in ABTS and FRAP assays. Conclusions: These findings suggest that L. sakei and L. curvatus have significant antioxidant and anti-inflammatory properties, highlighting their potential as probiotics and prebiotics. Moreover, these newly isolated strains from GRET are expected to serve as valuable functional ingredients for developing health-promoting foods and dietary supplements. Full article
(This article belongs to the Section Prebiotics and Probiotics)
27 pages, 1434 KiB  
Review
Unmasking the Epigenome: Insights into Testicular Cell Dynamics and Reproductive Function
by Shabana Anjum, Yamna Khurshid, Stefan S. Du Plessis and Temidayo S. Omolaoye
Int. J. Mol. Sci. 2025, 26(15), 7305; https://doi.org/10.3390/ijms26157305 - 28 Jul 2025
Abstract
The epigenetic landscape plays a pivotal role in regulating the functions of both germ and somatic cells (Sertoli and Leydig cells) within the testis, which are essential for male fertility. While somatic cells support germ cell maturation and testosterone synthesis, the epigenetic regulation [...] Read more.
The epigenetic landscape plays a pivotal role in regulating the functions of both germ and somatic cells (Sertoli and Leydig cells) within the testis, which are essential for male fertility. While somatic cells support germ cell maturation and testosterone synthesis, the epigenetic regulation of germ cells is critical for proper spermatogenesis and function. Epigenetic modifications such as DNA methylation, histone modifications, chromatin remodeling, and non-coding RNAs (ncRNAs) are crucial for regulating gene expression that is essential for spermatogenesis and reproductive function. Although numerous studies have highlighted the significance of the epigenome and its implications for male reproductive health, a comprehensive overview of the existing literature and knowledge is lacking. This review aims to provide an in-depth analysis of the role of epigenetics in spermatogenesis and reproductive health, with a specific focus on DNA methylation, histone remodeling, and small noncoding RNAs (sncRNAs). Additionally, we examine the impact of lifestyle and environmental factors, such as diet, smoking, physical activity, and exposure to endocrine-disrupting chemicals, on the sperm epigenome. We emphasize how these factors influence fertility, embryonic development, and potential transgenerational inheritance. This review underscores how recent advances in the understanding of the epigenetic modulation of testicular function can inform the pathophysiology of male infertility, thereby paving the way for the development of targeted diagnostic and therapeutic strategies. Full article
(This article belongs to the Special Issue Advances in Spermatogenesis and Male Infertility)
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18 pages, 4204 KiB  
Article
Audouin’s Gull Colony Itinerancy: Breeding Districts as Units for Monitoring and Conservation
by Massimo Sacchi, Barbara Amadesi, Adriano De Faveri, Gilles Faggio, Camilla Gotti, Arnaud Ledru, Sergio Nissardi, Bernard Recorbet, Marco Zenatello and Nicola Baccetti
Diversity 2025, 17(8), 526; https://doi.org/10.3390/d17080526 - 28 Jul 2025
Abstract
We investigated the spatial structure and colony itinerancy of Audouin’s gull (Ichthyaetus audouinii) adult breeders across multiple breeding sites in the central Mediterranean Sea during 25 years of fieldwork. Using cluster analysis of marked individuals from different years and sites, we [...] Read more.
We investigated the spatial structure and colony itinerancy of Audouin’s gull (Ichthyaetus audouinii) adult breeders across multiple breeding sites in the central Mediterranean Sea during 25 years of fieldwork. Using cluster analysis of marked individuals from different years and sites, we identified five spatial breeding units of increasing hierarchical scale—Breeding Sites, Colonies, Districts, Regions and Marine Sectors—which reflect biologically meaningful boundaries beyond simple geographic proximity. To determine the most appropriate scale for monitoring local populations, we applied multievent capture–recapture models and examined variation in survival and site fidelity across these units. Audouin’s gulls frequently change their location at the Breeding Site and Colony levels from one year to another, without apparent survival costs. In contrast, dispersal beyond Districts boundaries was found to be rare and associated with reduced survival rates, indicating that breeding Districts represent the most relevant biological unit for identifying local populations. The survival disadvantage observed in individuals leaving their District likely reflects increased extrinsic mortality in unfamiliar environments and the selective dispersal of lower-quality individuals. Within breeding Districts, birds may benefit from local knowledge and social information, supporting demographic stability and higher fitness. Our findings highlight the value of adopting a District-based framework for long-term monitoring and conservation of this endangered species. At this scale, demographic trends such as population growth or decline emerge more clearly than when assessed at the level of singular colonies. This approach can enhance our understanding of population dynamics in other mobile species and support more effective conservation strategies aligned with natural population structure. Full article
(This article belongs to the Special Issue Ecology, Diversity and Conservation of Seabirds—2nd Edition)
27 pages, 1332 KiB  
Article
Generalizing Coherent States with the Fox H Function
by Filippo Giraldi
Quantum Rep. 2025, 7(3), 33; https://doi.org/10.3390/quantum7030033 - 28 Jul 2025
Abstract
In the present scenario, coherent states of a quantum harmonic oscillator are generalized with positive Fox H auxiliary functions. The novel generalized coherent states provide canonical coherent states and Mittag-Leffler or Wright generalized coherent states, as particular cases, and resolve the identity operator, [...] Read more.
In the present scenario, coherent states of a quantum harmonic oscillator are generalized with positive Fox H auxiliary functions. The novel generalized coherent states provide canonical coherent states and Mittag-Leffler or Wright generalized coherent states, as particular cases, and resolve the identity operator, over the Fock space, with a weight function that is the product of a Fox H function and a Wright generalized hypergeometric function. The novel generalized coherent states, or the corresponding truncated generalized coherent states, are characterized by anomalous statistics for large values of the number of excitations: the corresponding decay laws exhibit, for determined values of the involved parameters, various behaviors that depart from exponential and inverse-power-law decays, or their product. The analysis of the Mandel Q factor shows that, for small values of the label, the statistics of the number of excitations becomes super-Poissonian, or sub-Poissonian, by simply choosing sufficiently large values of one of the involved parameters. The time evolution of a generalized coherent state interacting with a thermal reservoir and the purity are analyzed. Full article
(This article belongs to the Special Issue Exclusive Feature Papers of Quantum Reports in 2024–2025)
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10 pages, 609 KiB  
Communication
Scalable Synthesis of 2D TiNCl via Flash Joule Heating
by Gabriel A. Silvestrin, Marco Andreoli, Edson P. Soares, Elita F. Urano de Carvalho, Almir Oliveira Neto and Rodrigo Fernando Brambilla de Souza
Physchem 2025, 5(3), 30; https://doi.org/10.3390/physchem5030030 - 28 Jul 2025
Abstract
A scalable synthesis of two-dimensional titanium nitride chloride (TiNCl) via flash Joule heating (FJH) using titanium tetrachloride (TiCl4) precursor has been developed. This single-step method overcomes traditional synthesis challenges, including high energy consumption, multi-step procedures, and hazardous reagent requirements. The structural [...] Read more.
A scalable synthesis of two-dimensional titanium nitride chloride (TiNCl) via flash Joule heating (FJH) using titanium tetrachloride (TiCl4) precursor has been developed. This single-step method overcomes traditional synthesis challenges, including high energy consumption, multi-step procedures, and hazardous reagent requirements. The structural and chemical properties of the synthesized TiNCl were characterized through multiple analytical techniques. X-ray diffraction (XRD) patterns confirmed the presence of TiNCl phase, while Raman spectroscopy data showed no detectable oxide impurities. Fourier transform infrared spectroscopy (FTIR) analysis revealed characteristic Ti–N stretching vibrations, further confirming successful titanium nitride synthesis. Transmission electron microscopy (TEM) imaging revealed thin, plate-like nanostructures with high electron transparency. These analyses confirmed the formation of highly crystalline TiNCl flakes with nanoscale dimensions and minimal structural defects. The material exhibits excellent structural integrity and phase purity, demonstrating potential for applications in photocatalysis, electronics, and energy storage. This work establishes FJH as a sustainable and scalable approach for producing MXenes with controlled properties, facilitating their integration into emerging technologies. Unlike conventional methods, FJH enables rapid, energy-efficient synthesis while maintaining material quality, providing a viable route for industrial-scale production of two-dimensional materials. Full article
(This article belongs to the Section Nanoscience)
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23 pages, 3051 KiB  
Article
Digital Inequality and Smart Inclusion: A Socio-Spatial Perspective from the Region of Xanthi, Greece
by Kyriaki Kourtidou, Yannis Frangopoulos, Asimenia Salepaki and Dimitris Kourkouridis
Smart Cities 2025, 8(4), 123; https://doi.org/10.3390/smartcities8040123 - 28 Jul 2025
Abstract
This study explores digital inequality as a socio-spatial phenomenon within the context of smart inclusion, focusing on the Regional Unit of Xanthi, Greece—a region marked by ethno-cultural diversity and pronounced urban–rural contrasts. Using a mixed-methods design, this research integrates secondary quantitative data with [...] Read more.
This study explores digital inequality as a socio-spatial phenomenon within the context of smart inclusion, focusing on the Regional Unit of Xanthi, Greece—a region marked by ethno-cultural diversity and pronounced urban–rural contrasts. Using a mixed-methods design, this research integrates secondary quantitative data with qualitative insights from semi-structured interviews, aiming to uncover how spatial, demographic, and cultural variables shape digital engagement. Geographic Information System (GIS) tools are employed to map disparities in internet access and ICT infrastructure, revealing significant gaps linked to geography, education, and economic status. The findings demonstrate that digital inequality is particularly acute in rural, minority, and economically marginalized communities, where limited infrastructure intersects with low digital literacy and socio-economic disadvantage. Interview data further illuminate how residents navigate exclusion, emphasizing generational divides, perceptions of technology, and place-based constraints. By bridging spatial analysis with lived experience, this study advances the conceptualization of digitally inclusive smart regions. It offers policy-relevant insights into how territorial inequality undermines the goals of smart development and proposes context-sensitive interventions to promote equitable digital participation. The case of Xanthi underscores the importance of integrating spatial justice into smart city and regional planning agendas. Full article
17 pages, 7377 KiB  
Article
Comparative Untargeted Metabolomic Analysis of Fruiting Bodies from Three Sanghuangporus Species
by Zixuan Jiang, Shimao Chen, Jia Song, Tao Xie, Yu Xue and Qingshan Yang
J. Fungi 2025, 11(8), 558; https://doi.org/10.3390/jof11080558 - 28 Jul 2025
Abstract
Sanghuangporus spp. are medicinal fungi with significant therapeutic value, but their taxonomic ambiguity and frequent market adulteration have hindered their standardized utilization. In this study, untargeted metabolomics based on UPLC-Q-TOF-MS was employed to systematically analyze the metabolic profiles of three Sanghuangporus species: Sanghuangporus [...] Read more.
Sanghuangporus spp. are medicinal fungi with significant therapeutic value, but their taxonomic ambiguity and frequent market adulteration have hindered their standardized utilization. In this study, untargeted metabolomics based on UPLC-Q-TOF-MS was employed to systematically analyze the metabolic profiles of three Sanghuangporus species: Sanghuangporus. sanghuang (SS), Sanghuangporus. vaninii (SV), and Sanghuangporus. baumii (SB). A total of 788 metabolites were identified and classified into 16 categories, among which 97 were common differential metabolites, including bioactive compounds such as flavonoids, polysaccharides, and terpenoids. Multivariate statistical analyses (PCA and OPLS-DA) revealed distinct metabolic patterns among the species. KEGG pathway enrichment analysis showed that the differential metabolites were mainly involved in flavonoid and isoflavonoid biosynthesis. Notably, SV and SB exhibited significantly higher levels of several key bioactive compounds, including Apigenin and D-glucuronolactone, compared to SS. These findings highlight substantial interspecies differences in metabolic composition and pharmacological potential, providing a scientific basis for species authentication, quality control, and medicinal development of Sanghuangporus. Full article
(This article belongs to the Special Issue Bioactive Secondary Metabolites from Fungi)
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24 pages, 6519 KiB  
Article
The Luminous Ambience of an Ancient Roman Public Building: A Characterization of the Inner Daylit Environment of Rogatianus Library in Thamugadi City (Timgad, Algeria)
by Hana Djouadi, Azeddine Belakehal and Paola Zanovello
Heritage 2025, 8(8), 300; https://doi.org/10.3390/heritage8080300 - 28 Jul 2025
Abstract
The Roman public library of Timgad (Algeria) constituted the study object of several extensive research works, particularly during the French colonial era. Following a virtual restitution-focused research work, this investigation aims to quantitatively characterize the daylighting conditions inside Timgad Public Library. Here, it [...] Read more.
The Roman public library of Timgad (Algeria) constituted the study object of several extensive research works, particularly during the French colonial era. Following a virtual restitution-focused research work, this investigation aims to quantitatively characterize the daylighting conditions inside Timgad Public Library. Here, it must be remembered that the luminous environment inside libraries is a main design parameter and a main environmentalfactor. In addition, it must be highlighted that the Timgad region’s luminous climate differs from where Rome’s designers and builders practiced. Hence, at a first step, a comparison is carried out between the precepts of Vitruvius, the pioneer of ancient Roman architecture, and the outcomes of previous studies related to Roman libraries. Then, as a second step, a double approach combining both 3D geometric modeling and numerical simulation using Radiance (2.0Beta) software. These simulations are mainly elaborated for the case of the large reading room, including most of the building’s main activities—consulting, reading, and storing books. Finally, this inner luminous environment’s characterization highlights that the Timgad Roman antic library was not uniformly daylit and suggests that its use varied spatially and temporally with respect to this environmental parameter. Full article
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16 pages, 957 KiB  
Article
The Influence of Blood Transfusion Indexed to Patient Blood Volume on 5-Year Mortality After Coronary Artery Bypass Grafting—An EuroSCORE II Adjusted Spline Regression Analysis
by Joseph Kletzer, Maximilian Kreibich, Martin Czerny, Tim Berger, Albi Fagu, Laurin Micek, Ulrich Franke, Matthias Eschenhagen, Tau S. Hartikainen, Mirjam Wild and Dalibor Bockelmann
J. Cardiovasc. Dev. Dis. 2025, 12(8), 287; https://doi.org/10.3390/jcdd12080287 - 28 Jul 2025
Abstract
Background: While timely blood transfusion is critical for restoring oxygen-carrying capacity after coronary artery bypass grafting (CABG), allogeneic blood product transfusions are independently associated with increased long-term mortality, necessitating a risk-stratified approach to balance oxygen delivery against immunological complications and infection risks. Methods: [...] Read more.
Background: While timely blood transfusion is critical for restoring oxygen-carrying capacity after coronary artery bypass grafting (CABG), allogeneic blood product transfusions are independently associated with increased long-term mortality, necessitating a risk-stratified approach to balance oxygen delivery against immunological complications and infection risks. Methods: We retrospectively analyzed 3376 patients undergoing isolated CABG between 2005 and 2023 at a single tertiary center. Patients who died during their perioperative hospital stay within 30 days were excluded. Transfusion burden was assessed both as the absolute number of blood product units (packed red blood cells, platelet transfusion, fresh frozen plasma) and as a percentage of calculated patient blood volume. The primary outcome was all-cause mortality at 5 years. Flexible Cox regression with penalized smoothing splines, adjusted for EuroSCORE II, was used to model dose–response relationships. Results: From our cohort of 3376 patients, a total of 137 patients (4.05%) received >10 units of packed red blood cells (PRBC) perioperatively. These patients were older (median 71 vs. 68 years, p < 0.001), more often female (29% vs. 15%, p < 0.001), and had higher preoperative risk (EuroSCORE II: 2.53 vs. 1.41, p < 0.001). After 5 years, mortality was 42% in the massive transfusion group versus 10% in controls. Spline regression revealed an exponential increase in mortality with transfused units: 14 units yielded a 1.5-fold higher hazard of death (HR 1.46, 95% CI 1.31–1.64), rising to HR 2.71 (95% CI 2.12–3.47) at 30 units. When transfusion was indexed to blood volume, this relationship became linear and more tightly correlated with mortality, with lower maximum hazard ratios and narrower confidence intervals. Conclusion: Indexing transfusion burden to the percentage of patient blood volume replaced provides a more accurate and clinically actionable predictor of 5-year mortality after CABG than absolute unit counts. Our findings support a shift toward individualized, volume-based transfusion strategies to optimize patient outcomes and resource stewardship in a time of limited availability of blood products. Full article
(This article belongs to the Special Issue 2nd Edition: Interventional Therapies and Management in Coronary Artery Disease)
19 pages, 395 KiB  
Article
Exploration of Client Experiences of Veterinary Care for Companion Animals (Dogs and Cats) and the Impact of Trauma-Informed Care on Client Outcomes
by Vanessa I. Rohlf, Nadia Manfrenuzzi, Neelofar Rehman and Pauleen C. Bennett
Vet. Sci. 2025, 12(8), 709; https://doi.org/10.3390/vetsci12080709 - 28 Jul 2025
Abstract
Trauma-informed care (TIC) is an increasingly implemented approach in human services, but little research documents implementation within veterinary services. This study explored emotional experiences of veterinary clients and the perceived emotions/behaviors of their pets, studied experiences of TIC, and ascertained the extent to [...] Read more.
Trauma-informed care (TIC) is an increasingly implemented approach in human services, but little research documents implementation within veterinary services. This study explored emotional experiences of veterinary clients and the perceived emotions/behaviors of their pets, studied experiences of TIC, and ascertained the extent to which experiences of TIC predicted client outcomes. Clients (N = 274) were surveyed about their experiences of veterinary care. Clients documented positive and negative emotional experiences, with negative emotions associated with more serious presenting issues. TIC was experienced during veterinary visits. Principal component analyses found that TIC fell into the following five factors: (1) transparent and client-centered communication; (2) client supports and strengths; (3) emotional safety and empowerment; (4) physical safety and comfort; (5) informed consent. Hierarchical regressions revealed that, after controlling for reason for the visit, client-reported emotions and pet emotions/behaviors, 53.9% and 56.3% of the variance in client satisfaction and client disappointment was accounted for by TIC practices, respectively. The results show that although the outcomes for pets receiving veterinary care might not always be positive, which can lead to negative emotional experiences for clients, these can be ameliorated and lead to positive client outcomes through utilizing TIC. Full article
24 pages, 2389 KiB  
Article
CT-Based Habitat Radiomics Combining Multi-Instance Learning for Early Prediction of Post-Neoadjuvant Lymph Node Metastasis in Esophageal Squamous Cell Carcinoma
by Qinghe Peng, Shumin Zhou, Runzhe Chen, Jinghui Pan, Xin Yang, Jinlong Du, Hongdong Liu, Hao Jiang, Xiaoyan Huang, Haojiang Li and Li Chen
Bioengineering 2025, 12(8), 813; https://doi.org/10.3390/bioengineering12080813 - 28 Jul 2025
Abstract
Early prediction of lymph node metastasis (LNM) following neoadjuvant therapy (NAT) is crucial for timely treatment optimization in esophageal squamous cell carcinoma (ESCC). This study developed and validated a computed tomography-based radiomic model for predicting pathologically confirmed LNM status at the time of [...] Read more.
Early prediction of lymph node metastasis (LNM) following neoadjuvant therapy (NAT) is crucial for timely treatment optimization in esophageal squamous cell carcinoma (ESCC). This study developed and validated a computed tomography-based radiomic model for predicting pathologically confirmed LNM status at the time of surgery in ESCC patients after NAT. A total of 469 ESCC patients from Sun Yat-sen University Cancer Center were retrospectively enrolled and randomized into a training cohort (n = 328) and a test cohort (n = 141). Three signatures were constructed: the tumor-habitat-based signature (Habitat_Rad), derived from radiomic features of three tumor subregions identified via K-means clustering; the multiple instance learning-based signature (MIL_Rad), combining features from 2.5D deep learning models; and the clinicoradiological signature (Clinic), developed through multivariate logistic regression. A combined radiomic nomogram integrating these signatures outperformed the individual models, achieving areas under the curve (AUCs) of 0.929 (95% CI, 0.901–0.957) and 0.852 (95% CI, 0.778–0.925) in the training and test cohorts, respectively. The decision curve analysis confirmed a high net clinical benefit, highlighting the nomogram’s potential for accurate LNM prediction after NAT and guiding individualized therapy. Full article
(This article belongs to the Special Issue Machine Learning Methods for Biomedical Imaging)
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17 pages, 1102 KiB  
Article
Topic Modeling Analysis of Children’s Food Safety Management Using BigKinds News Big Data: Comparing the Implementation Times of the Comprehensive Plan for Children’s Dietary Safety Management
by Hae Jin Park, Sang Goo Cho, Kyung Won Lee, Seung Jae Lee and Jieun Oh
Foods 2025, 14(15), 2650; https://doi.org/10.3390/foods14152650 - 28 Jul 2025
Abstract
As digital technologies and food environments evolve, ensuring children’s food safety has become a pressing public health priority. This study examines how the policy discourse on children’s dietary safety in Korea has shifted over time by applying Latent Dirichlet Allocation (LDA) topic modeling [...] Read more.
As digital technologies and food environments evolve, ensuring children’s food safety has become a pressing public health priority. This study examines how the policy discourse on children’s dietary safety in Korea has shifted over time by applying Latent Dirichlet Allocation (LDA) topic modeling to news articles from 2010 to 2024. Using a large-scale news database (BigKinds), the analysis identifies seven key themes that have emerged across five phases of the national Comprehensive Plans for Safety Management of Children’s Dietary Life. These include experiential education, data-driven policy approaches, safety-focused meal management, healthy dietary environments, nutritional support for children’s growth, customized safety education, and private-sector initiatives. A significant increase in digital keywords—such as “big data” and “artificial intelligence”—highlights a growing emphasis on data-oriented policy tools. By capturing the evolving language and priorities in food safety policy, this study provides new insights into the digital transformation of public health governance and offers practical implications for adaptive and technology-informed policy design. Full article
(This article belongs to the Section Food Quality and Safety)
13 pages, 686 KiB  
Article
The Performance of Pleurotus eryngii β-Glucans on Protein Digestion and the Release of Free Amino Acids in the Bloodstream of Obese Adults
by Charalampia Amerikanou, Stamatia-Angeliki Kleftaki, Aristea Gioxari, Dimitra Tagkouli, Alexandra Kasoura, Stamatia Simati, Chara Tzavara, Alexander Kokkinos, Nick Kalogeropoulos and Andriana C. Kaliora
Foods 2025, 14(15), 2649; https://doi.org/10.3390/foods14152649 - 28 Jul 2025
Abstract
Pleurotus eryngii is an edible mushroom with previously characterized β-glucans. Its potential to ameliorate postprandial glycemia and regulate appetite at the postprandial state has been previously shown. However, its effect on protein digestion remains unexplored. We aimed to investigate the effect of baked [...] Read more.
Pleurotus eryngii is an edible mushroom with previously characterized β-glucans. Its potential to ameliorate postprandial glycemia and regulate appetite at the postprandial state has been previously shown. However, its effect on protein digestion remains unexplored. We aimed to investigate the effect of baked P. eryngii with a known β-glucan content (4.5 g) on plasma free amino acids of patients with central obesity and metabolic abnormalities at a postprandial state. In this acute, randomized controlled cross-over study, thirteen healthy male volunteers consumed one meal that was prepared with P. eryngii and one control meal; each meal was separated by one month. Blood was collected, and plasma was isolated at different timepoints before and after the consumption. Gas chromatography–mass spectrometry was used to quantify 24 free amino acids in the plasma samples. The area under the curve with respect to increase (AUCi) was computed, and the AUCi for aromatic amino acids was found to be higher after the consumption of the control meal compared to the P. eryngii meal (p = 0.027 for phenylalanine, p = 0.008 for tyrosine, and p = 0.003 for tryptophan). The above novel findings suggest that the β-glucans present in P. eryngii mushrooms are potential modulators of AA release into the bloodstream. Full article
(This article belongs to the Special Issue Food Bioactive Compounds in Chronic Diseases Prevention and Ageing)
13 pages, 1130 KiB  
Article
Feasibility and Preliminary Results of a Standardized Stair Climbing Test to Evaluate Cardiorespiratory Fitness in Children and Adolescents in a Non-Clinical Setting: The “Hand Aufs Herz” Study
by Federico Morassutti Vitale, Jennifer Wieprecht, Maren Baethmann, Delphina Gomes, Anja Tengler, Roxana Riley, Samar Shamas, Marcel Müller, Guido Mandilaras, Simone Katrin Manai, Maria Jaros, Nikolaus Alexander Haas and Meike Schrader
Children 2025, 12(8), 993; https://doi.org/10.3390/children12080993 - 28 Jul 2025
Abstract
Background/Objectives: Cardiorespiratory fitness (CRF) is of great interest in children and adolescents. Due to the limited availability of cardiopulmonary exercise testing, simple and reliable alternatives are needed. A stair climbing test (SCT) for the assessment of CRF developed at the Department of [...] Read more.
Background/Objectives: Cardiorespiratory fitness (CRF) is of great interest in children and adolescents. Due to the limited availability of cardiopulmonary exercise testing, simple and reliable alternatives are needed. A stair climbing test (SCT) for the assessment of CRF developed at the Department of Pediatric Cardiology of the LMU University Hospital in Munich showed a strong correlation with VO2max. The aim of this study is to prove its feasibility in a non-clinical setting and to analyse its results in a larger study population. Methods: During the “Hand aufs Herz” study, a comprehensive cardiovascular examination was carried out on 922 pupils and siblings (13.2 ± 7.8 years) at a high school in Bavaria. The SCT was performed to evaluate CRF: participants had to run up and down a total of four floors (14.8 m) as quickly as possible without skipping steps or holding on to the banister. Absolute time has been normalized over the standard height of 12 m to allow comparisons with different settings. An SCT Index was calculated to adjust results to the different weights of participants and the exact height of the staircase. Results: The SCT proved to be easily feasible and safe in non-clinical contexts. Out of 922 participants, 13 (1.4%) were not able to perform the test, and 3 (0.3%) had to interrupt it following fatigue or stumbling. A total of 827 participants aged from 9 to 17 years (13.1 ± 2.1 years, 45.8% girls) had a mean absolute SCT time of 53.4 ± 6.2 s and 43.3 ± 5.1 s when normalized over 12 m. Conclusions: The SCT represents a simple, cost- and time-saving test that allows a rapid and solid assessment of cardiorespiratory fitness in children and adolescents. We could demonstrate that it is safe and feasible in non-clinical contexts. Its short duration and universal applicability are valuable advantages that could facilitate the establishment of a repetitive cardiovascular screening in the pediatric population, particularly in outpatient departments or settings with low-resource systems. Full article
(This article belongs to the Special Issue Prevention of Cardiovascular Diseases in Children and Adolescents)
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14 pages, 583 KiB  
Article
Unhealthy Ultra-Processed Food, Diet Quality and Adherence to the Mediterranean Diet in Children and Adolescents: The DELICIOUS Project
by Francesca Giampieri, Alice Rosi, Evelyn Frias-Toral, Osama Abdelkarim, Mohamed Aly, Achraf Ammar, Raynier Zambrano-Villacres, Juancho Pons, Laura Vázquez-Araújo, Nunzia Decembrino, Alessandro Scuderi, Alice Leonardi, Lorenzo Monasta, Fernando Maniega Legarda, Ana Mata, Adrián Chacón, Pablo Busó and Giuseppe Grosso
Foods 2025, 14(15), 2648; https://doi.org/10.3390/foods14152648 - 28 Jul 2025
Abstract
Background: Western dietary patterns worldwide are increasingly dominated by energy-dense, nutrient-deficient industrial foods, often identified as ultra-processed foods (UPFs). Such products may have detrimental health implications, particularly if nutritionally inadequate. This study aimed to examine the intake of unhealthy UPFs among children and [...] Read more.
Background: Western dietary patterns worldwide are increasingly dominated by energy-dense, nutrient-deficient industrial foods, often identified as ultra-processed foods (UPFs). Such products may have detrimental health implications, particularly if nutritionally inadequate. This study aimed to examine the intake of unhealthy UPFs among children and adolescents from five Mediterranean countries (Italy, Spain, Portugal, Egypt, and Lebanon) involved in the DELICIOUS project and to assess the association with dietary quality indicators. Methods: A survey was conducted with a sample of 2011 parents of children and adolescents aged 6 to 17 years to evaluate their dietary habits. Diet quality was assessed using the Youth Healthy Eating Index (Y-HEI), the KIDMED index to determine adherence to the Mediterranean diet, and compliance with national dietary guidelines. Results: Increased UPF consumption was not inherently associated with healthy or unhealthy specific food groups, although children and adolescents who consumed UPF daily were less likely to exhibit high overall diet quality and adherence to the Mediterranean diet. In all five countries, greater UPF intake was associated with poorer compliance with dietary recommendations concerning fats, sweets, meat, and legumes. Conclusions: Increased UPF consumption among Mediterranean children and adolescents is associated with an unhealthy dietary pattern, possibly marked by a high intake of fats, sweets, and meat, and a low consumption of legumes. Full article
(This article belongs to the Special Issue Food Habits, Nutritional Knowledge, and Nutrition Education)
27 pages, 4299 KiB  
Article
Causal Relationship Between Serum Uric Acid and Atherosclerotic Disease: A Mendelian Randomization and Transcriptomic Analysis
by Shitao Wang, Shuai Mei, Xiaozhu Ma, Qidamugai Wuyun, Li Zhou, Qiushi Luo, Ziyang Cai and Jiangtao Yan
Biomedicines 2025, 13(8), 1838; https://doi.org/10.3390/biomedicines13081838 - 28 Jul 2025
Abstract
Background/Objectives: Elevated serum uric acid levels are associated with the occurrence, development, and adverse events of coronary heart disease (CHD) and CHD risk factors. However, the extent of any pathogenic effect of the serum uric acid on CHD and whether CHD risk [...] Read more.
Background/Objectives: Elevated serum uric acid levels are associated with the occurrence, development, and adverse events of coronary heart disease (CHD) and CHD risk factors. However, the extent of any pathogenic effect of the serum uric acid on CHD and whether CHD risk factors play a confounding or mediating role are still unclear. Methods: The potential causal associations of serum uric acid with CHD were evaluated via cross-trait linkage disequilibrium score regression analysis and Mendelian randomization. The pleiotropy of genetic tools was analyzed via a Bayesian colocalization approach. Moreover, we utilized two-step MR to identify risk factors mediating the relationship between uric acid and CHD. Results: Mendelian randomization results derived from two genetic instrument selection strategies support that serum uric acid levels have a significant causal relationship with coronary artery disease, stable angina pectoris, and myocardial infarction. This causal relationship was partially mediated by diastolic blood pressure, mean arterial pressure, and serum triglycerides. Transcriptomic analysis revealed that serum uric acid may directly contribute to the development of atherosclerosis by inducing transcriptomic changes in macrophages. Conclusions: Our findings highlight that the control of serum urate concentration in the long-term management of CHD patients may be necessary. Well-designed clinical trials and foundational research are presently required to furnish conclusive proof regarding the specific clinical scenarios in which adequate reduction in urate concentrations can confer cardiovascular advantages. Full article
(This article belongs to the Special Issue Advances in Genomics and Bioinformatics of Human Disease)
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15 pages, 1236 KiB  
Article
T-Lymphocyte Phenotypic and Mitochondrial Parameters as Markers of Incomplete Immune Restoration in People Living with HIV+ on Long-Term cART
by Damian Vangelov, Radoslava Emilova, Yana Todorova, Nina Yancheva, Reneta Dimitrova, Lyubomira Grigorova, Ivailo Alexiev and Maria Nikolova
Biomedicines 2025, 13(8), 1839; https://doi.org/10.3390/biomedicines13081839 - 28 Jul 2025
Abstract
Background/Objectives: Restored CD4 absolute counts (CD4AC) and CD4/CD8 ratio in the setting of continuous antiretroviral treatment (ART) do not exclude a low-level immune activation associated with HIV reservoirs, microbial translocation, or the side effects of ART itself, which accelerates the aging of [...] Read more.
Background/Objectives: Restored CD4 absolute counts (CD4AC) and CD4/CD8 ratio in the setting of continuous antiretroviral treatment (ART) do not exclude a low-level immune activation associated with HIV reservoirs, microbial translocation, or the side effects of ART itself, which accelerates the aging of people living with HIV (PLHIV). To delineate biomarkers of incomplete immune restoration in PLHIV on successful ART, we evaluated T-lymphocyte mitochondrial parameters in relation to phenotypic markers of immune exhaustion and senescence. Methods: PLHIV with sustained viral suppression, CD4AC >500 and CD4/CD8 ratio >0.9 on ART (n = 39) were compared to age-matched ART-naïve donors (n = 27) and HIV(–) healthy controls (HC, n = 35). CD4 and CD8 differentiation and effector subsets (CCR7/CD45RA and CD27/CD28), activation, exhaustion, and senescence markers (CD38, CD39 Treg, CD57, TIGIT, and PD-1) were determined by flow cytometry. Mitochondrial mass (MM) and membrane potential (MMP) of CD8 and CD4 T cells were evaluated with MitoTracker Green and Red flow cytometry dyes. Results: ART+PLHIV differed from HC by increased CD4 TEMRA (5.3 (2.1–8.8) vs. 3.2 (1.6–4.4), p < 0.05), persistent TIGIT+CD57–CD27+CD28– CD8+ subset (53.9 (45.5–68.9) vs. 40.1 (26.7–58.5), p < 0.05), and expanding preapoptotic TIGIT–CD57+CD8+ effectors (9.2 (4.3–21.8) vs. 3.0 (1.5–7.3), p < 0.01) in correlation with increased CD8+ MMP (2527 (1675–4080) vs.1477 (1280–1691), p < 0.01). These aberrations were independent of age, time to ART, or ART duration, and were combined with increasing CD4 T cell MMP and MM. Conclusions: In spite of recovered CD4AC and CD4/CD8 ratio, the increased CD8+ MMP, combined with elevated markers of exhaustion and senescence in ART+PLHIV, signals a malfunction of the CD8 effector pool that may compromise viral reservoir latency. Full article
(This article belongs to the Special Issue Emerging Insights into HIV)
10 pages, 265 KiB  
Article
Horváth Spaces and a Representations of the Fourier Transform and Convolution
by Emilio R. Negrín, Benito J. González and Jeetendrasingh Maan
Mathematics 2025, 13(15), 2435; https://doi.org/10.3390/math13152435 - 28 Jul 2025
Abstract
This paper explores the structural representation and Fourier analysis of elements in Horváth distribution spaces Sk′, for k<−n. We prove that any element in Sk′ can be expressed as a finite sum of derivatives [...] Read more.
This paper explores the structural representation and Fourier analysis of elements in Horváth distribution spaces Sk′, for k<−n. We prove that any element in Sk′ can be expressed as a finite sum of derivatives of continuous L1(Rn)-functions acting on Schwartz test functions. This representation leads to an explicit expression for their distributional Fourier transform in terms of classical Fourier transforms. Additionally, we present a distributional representation for the convolution of two such elements, showing that the convolution is well-defined over S. These results deepen our understanding of non-tempered distributions and extend Fourier methods to a broader functional framework. Full article
(This article belongs to the Special Issue Special Functions with Applications)
19 pages, 4955 KiB  
Article
Wind-Induced Responses of Nonlinear Angular Motion for a Dual-Spin Rocket
by Jianwei Chen, Liangming Wang and Zhiwei Yang
Aerospace 2025, 12(8), 675; https://doi.org/10.3390/aerospace12080675 - 28 Jul 2025
Abstract
Fin-stabilized guided rockets exhibit ballistic characteristics such as low initial velocity, high flight altitude, and long flight duration, which render their impact point accuracy and flight stability highly susceptible to the influence of wind. In this paper, the four-dimensional nonlinear angular motion equations [...] Read more.
Fin-stabilized guided rockets exhibit ballistic characteristics such as low initial velocity, high flight altitude, and long flight duration, which render their impact point accuracy and flight stability highly susceptible to the influence of wind. In this paper, the four-dimensional nonlinear angular motion equations describing the changes in attack angle and the law of axis swing of a dual-spin rocket are established, and the phase trajectory and equilibrium point stability characteristics of the nonlinear angular motion system under windy conditions are analyzed. Aiming at the problem that the equilibrium point of the angular motion system cannot be solved analytically with the change in wind speed, a phase trajectory projection sequence method based on the Poincaré cross-section and stroboscopic mapping is proposed to analyze the effect of wind on the angular motion bifurcation characteristics of a dual-spin rocket. The possible instability of angular motion caused by nonlinear aerodynamics under strong wind conditions is explored. This study is of reference significance for the launch control and aerodynamic design of guided rockets in complex environments. Full article
(This article belongs to the Section Aeronautics)
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24 pages, 1007 KiB  
Review
Evaluating Antimicrobial Susceptibility Testing Methods for Cefiderocol: A Review and Expert Opinion on Current Practices and Future Directions
by Stefania Stefani, Fabio Arena, Luigi Principe, Stefano Stracquadanio, Chiara Vismara and Gian Maria Rossolini
Antibiotics 2025, 14(8), 760; https://doi.org/10.3390/antibiotics14080760 - 28 Jul 2025
Abstract
Background: Cefiderocol (FDC) presents challenges in antimicrobial susceptibility testing (AST). The reference standard is the broth microdilution (BMD) method with iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB). Still, it is cumbersome for routine clinical laboratory use, while variable accuracy has been reported with available commercial [...] Read more.
Background: Cefiderocol (FDC) presents challenges in antimicrobial susceptibility testing (AST). The reference standard is the broth microdilution (BMD) method with iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB). Still, it is cumbersome for routine clinical laboratory use, while variable accuracy has been reported with available commercial systems. Variability in interpretive criteria and areas of technical uncertainty (ATUs) further complicate assessments. Methods: This review and expert opinion presents: (1) an overview of non-susceptibility to FDC and then delves into the performance of current FDC AST methods for Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii complex; (2) a practical decision framework to guide clinical microbiologists in making informed choices. Results and Conclusions: For Enterobacterales, including carbapenem-resistant Enterobacterales (CRE), and Pseudomonas aeruginosa, we propose disk diffusion (DD) as a preliminary screening tool to classify isolates as susceptible (S) or resistant (R). Confirmatory testing using the UMIC® FDC system or the ID-CAMHB BMD method is recommended for R isolates. In cases of discrepancy, repeating the test with ID-CAMHB BMD is advised. Additionally, isolates falling within the ATU during DD testing should be retested using the UMIC® system or ID-CAMHB BMD. For A. baumannii complex, since EUCAST breakpoints have not been defined yet, we propose a stepwise framework based on the first DD result: isolates with inhibition zones <17 mm are considered non-susceptible and should be confirmed with standard BMD. Those between 17 and 22 mm require retesting with a commercial BMD method, with further confirmation recommended if S isolates with zones ≥23 mm may be considered S without additional testing. Full article
(This article belongs to the Special Issue Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing)
17 pages, 1139 KiB  
Review
Green Leafy Vegetables (GLVs) as Nutritional and Preventive Agents Supporting Metabolism
by Renata Nurzyńska-Wierdak
Metabolites 2025, 15(8), 502; https://doi.org/10.3390/metabo15080502 - 28 Jul 2025
Abstract
Metabolic syndrome (MetS) is defined as a group of metabolic defects that include hypertension, insulin resistance, visceral obesity, fatty liver disease, and atherosclerotic cardiovascular disease (CVD). The first step in controlling the progression of MetS is lifestyle changes, including dietary modification. Regular consumption [...] Read more.
Metabolic syndrome (MetS) is defined as a group of metabolic defects that include hypertension, insulin resistance, visceral obesity, fatty liver disease, and atherosclerotic cardiovascular disease (CVD). The first step in controlling the progression of MetS is lifestyle changes, including dietary modification. Regular consumption of fruits, vegetables, whole grains and other plant foods negatively correlates with the risk of developing chronic diseases. Green leafy vegetables (GLVs) are a key element of healthy eating habits and an important source of vitamins C and E, carotenoids—mainly β-carotene and lutein—and minerals. This review discusses and summarizes the current knowledge on the health benefits of consuming GLVs in the prevention and treatment of MetS to provide a compendium for other researchers investigating new natural products. Full article
(This article belongs to the Special Issue Plants and Plant-Based Foods for Metabolic Disease Prevention)
33 pages, 1667 KiB  
Systematic Review
Vitamin D as a Modifiable Risk Factor in Schizophrenia a Systematic Review
by Jadwiga Mosiołek, Bartosz Mosiołek and Agata Szulc
Biomolecules 2025, 15(8), 1094; https://doi.org/10.3390/biom15081094 - 28 Jul 2025
Abstract
The etiology of schizophrenia remains poorly understood. Although certain risk factors have been identified, effective preventive measures are still lacking. This study investigates potential preventive methods while focusing on the role of vitamin D and its status. The role of malnutrition in schizophrenia [...] Read more.
The etiology of schizophrenia remains poorly understood. Although certain risk factors have been identified, effective preventive measures are still lacking. This study investigates potential preventive methods while focusing on the role of vitamin D and its status. The role of malnutrition in schizophrenia risk was first identified in studies on the Dutch Hunger Winter. Vitamin D deficiency was hypothesized as a contributing factor shortly thereafter. This review aims to explore the correlations between vitamin D deficiency at various life stages (maternal, neonatal, adult) and schizophrenia risk, as well as its effects on pharmacokinetics, neurobiology, bone health, and metabolic syndrome. The studies were retrieved from two indexed databases, PubMed and Web of Science, following PRISMA guidelines and included studies published between 2000 and 2024. No correlation was found between maternal vitamin D levels and schizophrenia in offspring while a positive correlation was observed between low neonatal vitamin D levels and schizophrenia in later life. Approximately half of the studies on adults reported mean vitamin D concentrations of below 20 ng/mL which were negatively correlated with gray matter volume and bone health while positively correlated with the prevalence of metabolic syndrome. Additionally, vitamin D levels were also found to correlate with antipsychotic drug concentrations. Full article
(This article belongs to the Special Issue Biomarkers and Molecular Basis of Psychiatry)
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