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Life

Life is an international, peer-reviewed, open access journal of scientific studies related to fundamental themes in life sciences, from basic to applied research, published monthly online by MDPI. The Astrobiology Society of Britain (ASB) and Spanish Association for Cancer Research (ASEICA) are affiliated with Life and their members receive a discount on the article processing charges.

Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
High Visibility: indexed within Scopus, SCIE (Web of Science), PubMed, PMC, CAPlus / SciFinder, AGRIS, and other databases.
Journal Rank: JCR - Q1 (Biology) / CiteScore - Q1 (Paleontology)
Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 18 days after submission; acceptance to publication is undertaken in 2.5 days (median values for papers published in this journal in the first half of 2024).
Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
Testimonials: See what our editors and authors say about Life.
Companion journals for Life include: Physiologia and Hydrobiology.

Impact Factor: 3.2 (2023); 5-Year Impact Factor: 3.1 (2023)

Imprint Information Journal Flyer Open Access ISSN: 2075-1729

Latest Articles

14 pages, 1412 KiB

Open AccessReview

Regulation of Plant Growth and Development by Melatonin

by Dawei Shi, Lejia Zhao, Ruijia Zhang and Qiaofeng Song

Life 2024, 14(12), 1606; https://doi.org/10.3390/life14121606 (registering DOI) - 4 Dec 2024

Melatonin is a naturally occurring chemical with pleiotropic effects in various species. In plants, melatonin is associated with a variety of plant physiological processes, including plant growth and development, stress responses, etc. Thus, melatonin may hold promise for improving crop yields and agricultural [...] Read more.

Melatonin is a naturally occurring chemical with pleiotropic effects in various species. In plants, melatonin is associated with a variety of plant physiological processes, including plant growth and development, stress responses, etc. Thus, melatonin may hold promise for improving crop yields and agricultural sustainability. This review describes the biosynthetic mode of melatonin and its properties and summarizes its functions in growth, development, and reproduction. In addition, the role of melatonin in plants facing various stressful environments is elaborated upon, and its relationship with other phytohormones is summarized. Through this review, we recognize the problems and challenges facing melatonin research and propose some feasible solutions. Full article

(This article belongs to the Special Issue Plant Adaptation Mechanisms to Abiotic Stresses)

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26 pages, 11236 KiB

Open AccessArticle

Electroacupuncture Mitigates TRPV1 Overexpression in the Central Nervous System Associated with Fibromyalgia in Mice

by Anh Thi Ngoc Doan and Yi-Wen Lin

Life 2024, 14(12), 1605; https://doi.org/10.3390/life14121605 (registering DOI) - 4 Dec 2024

Background: Fibromyalgia (FM) is characterized by chronic pain, significantly affecting the quality of life and functional capabilities of patients. In addition to pain, patients may experience insomnia, chronic fatigue, depression, anxiety, and headaches, further complicating their overall well-being. The Transient Receptor Potential Vanilloid [...] Read more.

Background: Fibromyalgia (FM) is characterized by chronic pain, significantly affecting the quality of life and functional capabilities of patients. In addition to pain, patients may experience insomnia, chronic fatigue, depression, anxiety, and headaches, further complicating their overall well-being. The Transient Receptor Potential Vanilloid 1 (TRPV1) receptor responds to various noxious stimuli and plays a key role in regulating pain sensitivity and inflammation. Thus, targeting TRPV1 may provide analgesic and anti-inflammatory benefits. This study investigates the efficacy of electroacupuncture (EA) in alleviating chronic pain in FM through TRPV1 and its downstream molecules in the central nervous system (CNS). Methods: To model FM, we subjected mice to intermittent cold stress (ICS) for three days. The study comprised five rodent groups: Control (CON), ICS, ICS + EA, ICS + Sham EA, and ICS + KO (TRPV1 knockout mice). Results: Our findings revealed that ICS induced allodynia and hyperalgesia in mice by day four, persisting until day 21. EA at 2 Hz and TRPV1 KO significantly decreased both mechanical and thermal hypersensitivity (Withdrawal—Day 14: 2.43 ± 0.19 g; Day 21: 5.88 ± 0.47 g, n = 6, p < 0.05; Latency—Day 14: 2.77 ± 0.22 s; Day 21: 5.85 ± 0.41 s, n = 6, p < 0.05). In contrast, sham EA did not produce significant effects. Additionally, TRPV1 and several pain-related proteins were significantly elevated in the thalamus, somatosensory cortex (SSC), medial prefrontal cortex (mPFC), hippocampus, hypothalamus, cerebellum regions V (CB V), VI (CB VI) and VII (CB VII) after the ICS model. Both EA at the ST36 acupoint and TRPV1 KO mice showed diminished overexpression of pain-related proteins, with the sham EA group showing no significant changes compared to the ICS group. Conclusions: Chronic widespread pain was reduced by EA and TRPV1 KO, with the effects of EA on the TRPV1 pain pathway clearly evident in the CNS after 21 days. Full article

(This article belongs to the Special Issue Feature Paper in Physiology and Pathology: 2nd Edition)

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19 pages, 5757 KiB

Open AccessReview

Exploring Pediatric Dermatology in Skin of Color: Focus on Dermoscopy

by Emmanouil Karampinis, Olga Toli, Konstantina-Eirini Georgopoulou, Maria-Myrto Papadopoulou, Anna Vardiampasi, Efterpi Zafiriou, Elizabeth Lazaridou, Zoe Apalla, Aimilios Lallas, Biswanath Behera and Enzo Errichetti

Life 2024, 14(12), 1604; https://doi.org/10.3390/life14121604 (registering DOI) - 4 Dec 2024

This literature review aims to comprehensively evaluate the clinical and dermoscopic presentations of common pediatric diseases among children with skin of color (SoC) while also addressing potential variations based on racial backgrounds. This review encompasses various conditions, such as nevi subtypes, viral infections, [...] Read more.

This literature review aims to comprehensively evaluate the clinical and dermoscopic presentations of common pediatric diseases among children with skin of color (SoC) while also addressing potential variations based on racial backgrounds. This review encompasses various conditions, such as nevi subtypes, viral infections, infestations, and inflammatory dermatoses, as well as hair diseases and abnormal vascular formations, occurring in pediatric populations. Overall, we identified 7 studies on nevi subtypes, 24 studies on skin infections, 6 on inflammatory dermatoses, 10 on hair diseases and disorders, and 14 on miscellaneous disorders that also satisfied our SoC- and race-specific criteria. In case of no results, we assumed that dermoscopic findings are similar between SoC adults and children, confirming the hypothesis with our cases of dark-skinned Indian child patients. Inflammatory dermatoses such as psoriasis, eczema, and cutaneous mastocytosis, as well as skin infections like cutaneous leishmaniasis, appear with brownish backgrounds or exhibit dark structures more frequently than the respective dermoscopy images of Caucasian populations. Dermoscopy traits such as erythema in tinea capitis are uncommon or even absent on a dark-colored scalp, while a dark skin tone often obscures many characteristic features, such as dark and yellow dots in alopecia areata and even parts of an intradermal parasite in the case of scabies. Race-specific traits were also observed, such as corkscrew hair in tinea capitis, primarily seen in patients of African origin. Many dermoscopic images are consistent between SoC and non-SoC in various skin lesions, including vascular anomalies, juvenile xanthogranuloma, mastocytoma, and viral skin lesions like molluscum contagiosum, as well as in various hair disorders such as trichotillomania, while tinea capitis displays the most diverse reported dermoscopic features across SoC- and race-specific studies. Full article

(This article belongs to the Section Medical Research)

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4 pages, 170 KiB

Open AccessEditorial

Special Issue: “Novel Diagnosis and Treatment of Gastrointestinal Disease”

by Doina Georgescu and Felix Bratosin

Life 2024, 14(12), 1603; https://doi.org/10.3390/life14121603 - 4 Dec 2024

Significant advances in the diagnosis and treatment of gastrointestinal diseases have been made in recent years [...] Full article

(This article belongs to the Special Issue Novel Diagnosis and Treatment of Gastrointestinal Disease)

33 pages, 1356 KiB

Open AccessReview

Artificial Intelligence in the Non-Invasive Detection of Melanoma

by Banu İsmail Mendi, Kivanc Kose, Lauren Fleshner, Richard Adam, Bijan Safai, Banu Farabi and Mehmet Fatih Atak

Life 2024, 14(12), 1602; https://doi.org/10.3390/life14121602 - 4 Dec 2024

Skin cancer is one of the most prevalent cancers worldwide, with increasing incidence. Skin cancer is typically classified as melanoma or non-melanoma skin cancer. Although melanoma is less common than basal or squamous cell carcinomas, it is the deadliest form of cancer, with [...] Read more.

Skin cancer is one of the most prevalent cancers worldwide, with increasing incidence. Skin cancer is typically classified as melanoma or non-melanoma skin cancer. Although melanoma is less common than basal or squamous cell carcinomas, it is the deadliest form of cancer, with nearly 8300 Americans expected to die from it each year. Biopsies are currently the gold standard in diagnosing melanoma; however, they can be invasive, expensive, and inaccessible to lower-income individuals. Currently, suspicious lesions are triaged with image-based technologies, such as dermoscopy and confocal microscopy. While these techniques are useful, there is wide inter-user variability and minimal training for dermatology residents on how to properly use these devices. The use of artificial intelligence (AI)-based technologies in dermatology has emerged in recent years to assist in the diagnosis of melanoma that may be more accessible to all patients and more accurate than current methods of screening. This review explores the current status of the application of AI-based algorithms in the detection of melanoma, underscoring its potential to aid dermatologists in clinical practice. We specifically focus on AI application in clinical imaging, dermoscopic evaluation, algorithms that can distinguish melanoma from non-melanoma skin cancers, and in vivo skin imaging devices. Full article

(This article belongs to the Special Issue Noninvasive Skin Imaging: The Present and the Future in General Dermatology)

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16 pages, 1685 KiB

Open AccessArticle

Fertilization for Growth or Feeding the Weeds? A Deep Dive into Nitrogen’s Role in Rice Dynamics in Ecuador

by Cristhian Fernando Sánchez-Sabando, Adriana Beatriz Sánchez-Urdaneta, Fernando David Sánchez-Mora, Gary Eduardo Loor-Escobar and Barlin O. Olivares

Life 2024, 14(12), 1601; https://doi.org/10.3390/life14121601 - 4 Dec 2024

Rice (Oryza sativa L.) is a crucial crop for employment and agricultural output and heavily reliant on family labor. This study evaluated the effects of nitrogen levels (80, 120, and 160 kg·ha⁻¹) on weed incidence and key agronomic variables, including [...] Read more.

Rice (Oryza sativa L.) is a crucial crop for employment and agricultural output and heavily reliant on family labor. This study evaluated the effects of nitrogen levels (80, 120, and 160 kg·ha⁻¹) on weed incidence and key agronomic variables, including vegetative growth, yield, and related traits, in Ecuador’s primary rice-growing regions, Guayas and Los Ríos. A split-plot randomized complete block design was implemented using two rice varieties (INIAP-FL-Elite and SFL-11) and three planting densities (20 × 30, 25 × 30, and 30 × 30 cm). Weed incidence was higher in Los Ríos, dominated by grasses (55.28%), while Cyperaceae (46.27%) prevailed in Guayas. The data analysis included non-parametric tests to identify significant treatment effects, debiased sparse partial correlations (DSPCs) to reveal key agronomic interactions, and principal component analysis (PCA) to identify influential traits, ensuring robust and normalized interpretations. Analysis of variance indicated significant effects for all variables, with vegetative growth (VG) most affected (p < 0.001). The number of panicles (NP) and days to flowering (DF) showed significant though less pronounced effects, while the panicle length (LP) and 1000-seed weight (TSB) exhibited moderate responses. The DSPCs highlighted the grains per panicle (GP) and total biomass (SB) as critical variables, with significant correlations between the days to flowering and the tiller count at 55 days (r = 0.750, p < 0.001) and between the vegetative growth and the total biomass (r = 0.678, p < 0.001). PCA explained 58.8% of the total variance, emphasizing the days to flowering, plant height, total biomass, and yield as the most influential traits. These findings underline the importance of integrated nutrient and weed management strategies tailored to Ecuador’s agroecological conditions. Full article

(This article belongs to the Special Issue Plant Biotic and Abiotic Stresses 2024)

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10 pages, 414 KiB

Open AccessArticle

Do Patients with Rheumatoid Arthritis Have an (In)Adequate Level of Physical Activity? A Latent Class Analysis Approach

by Sretko Lukovic, Marko Baralic, Nina Tomonjic, Jovana Mihailovic, Aleksandra Neskovic, Marina Vujovic Sestakov, Ivana Pavlovic, Branko Barac, Tatjana Zivanovic Radnic and Predrag Ostojic

Life 2024, 14(12), 1600; https://doi.org/10.3390/life14121600 - 4 Dec 2024

Introduction: Regular physical activity (PA) has a beneficial effect on joint pain, stiffness, strength, flexibility, and aerobic capacity in patients with rheumatoid arthritis (RA). Objective: The aim of this study was to assess the level of PA in patients with rheumatoid arthritis and [...] Read more.

Introduction: Regular physical activity (PA) has a beneficial effect on joint pain, stiffness, strength, flexibility, and aerobic capacity in patients with rheumatoid arthritis (RA). Objective: The aim of this study was to assess the level of PA in patients with rheumatoid arthritis and to identify potential barriers to this activity. Material and Methods: The study involved 132 patients with RA. Participants completed the International Physical Activity Questionnaire (IPAQ), the Functional Assessment of Chronic Illness Therapy—Fatigue Scale (FACIT-F), the Tampa Scale for kinesiophobia (TSK), Strength, Ambulation, Rising from a chair, Stair climbing and history of Falling questionnaire (SARC-F) for sarcopenia assessment, and the Patient Health Questionnaire-9 (PHQ-9) for depression. Basic socio-epidemiological data, disease activity score in 28 joints (DAS₂₈), duration of disease, and therapy information were retrieved from electronic patient records. Latent class analysis (LCA) was used to identify subpopulations of patients. Results: The study included 109 women (82.6%) and 23 men (17.4%). Low levels of PA were observed in 16 patients (12%), moderate levels in 70 patients (53%), and high levels in 42 patients (35%). Symptoms of pronounced fatigue were significantly associated with low PA (28.5 ± 11.3 vs. 37 ± 7 vs. 37 ± 10; p = 0.002). The risk of sarcopenia was significantly higher in RA patients with low PA (p = 0.05). Kinesiophobia was present in all three groups (65.2%). LCA identified two classes. In the first class, patients were more likely to be non-exercisers compared to the second class. Patients in the first class were characterized by a higher probability of being female, obese, with lower education levels. Patients in the first class had pronounced fatigue, kinesiophobia and more frequent symptoms of depression. The second class (65% of the total population) included patients who exercised moderately to frequently (93%) and were middle-aged. They were less obese, highly educated, employed, and majority of them achieved low disease activity or remission. In addition, they had lower risks for sarcopenia, depression, fatigue, and kinesiophobia. Conclusions: This study showed that RA patients with moderate and high levels of PA have better disease control, fewer symptoms of fatigue and depression, and a lower risk of sarcopenia. However, kinesiophobia was significantly present in all three groups, indicating a need for further promotion of this non-pharmacological treatment. Full article

(This article belongs to the Section Epidemiology)

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14 pages, 1177 KiB

Open AccessArticle

Genome-Wide Identification and Characterization of OSC Gene Family in Gynostemma pentaphyllum (Cucurbitaceae)

by Xiao Zhang, Huan Yang, Xuan Wang, Xiaoting Wang and Chen Chen

Life 2024, 14(12), 1599; https://doi.org/10.3390/life14121599 - 4 Dec 2024

Gynostemma pentaphyllum is a traditional Chinese medicinal plant of considerable application value and commercial potential, primarily due to its production of various bioactive compounds, particularly dammarane-type triterpenoid saponins that are structurally analogous to ginsenosides. Oxidosqualene cyclase (OSC), a pivotal enzyme in the biosynthesis [...] Read more.

Gynostemma pentaphyllum is a traditional Chinese medicinal plant of considerable application value and commercial potential, primarily due to its production of various bioactive compounds, particularly dammarane-type triterpenoid saponins that are structurally analogous to ginsenosides. Oxidosqualene cyclase (OSC), a pivotal enzyme in the biosynthesis of triterpenoid metabolites in plants, catalyzes the conversion of oxidosqualene into triterpenoid precursors, which are essential components of the secondary metabolites found in G. pentaphyllum. To elucidate the role of OSC gene family members in the synthesis of gypenosides within G. pentaphyllum, this study undertook a comprehensive genome-wide identification and characterization of OSC genes within G. pentaphyllum and compared their expression levels across populations distributed over different geographical regions by both transcriptome sequencing and qRT-PCR experimental validation. The results identified a total of 11 members of the OSC gene family within the genome of G. pentaphyllum. These genes encode proteins ranging from 356 to 767 amino acids, exhibiting minor variations in their physicochemical properties, and are localized in peroxisomes, cytoplasm, plasma membranes, and lysosomes. All GpOSCs contain highly conserved DCTAE and QW sequences that are characteristic of the OSC gene family. A phylogenetic analysis categorized the GpOSCs into four distinct subfamilies. A cis-element analysis of the GpOSC promoters revealed a substantial number of abiotic stress-related elements, indicating that these genes may respond to drought conditions, low temperatures, and anaerobic environments, thus potentially contributing to the stress resistance observed in G. pentaphyllum. Expression analyses across different G. pentaphyllum populations demonstrated significant variability in OSC gene expression among geographically diverse samples of G. pentaphyllum, likely attributable to genetic variation or external factors such as environmental conditions and soil composition. These differences may lead to the synthesis of various types of gypenosides within geographically distinct G. pentaphyllum populations. The findings from this study enhance our understanding of both the evolutionary history of the OSC gene family in G. pentaphyllum and the biosynthetic mechanisms underlying triterpenoid compounds. This knowledge is essential for investigating molecular mechanisms involved in forming dammarane-type triterpenoid saponins as well as comprehending geographical variations within G. pentaphyllum populations. Furthermore, this research lays a foundation for employing plant genetic engineering techniques aimed at increasing gypenoside content. Full article

(This article belongs to the Special Issue The Genomics of Medicinal Plants)

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10 pages, 1555 KiB

Open AccessArticle

The Effects of Antioxidant Cocktail on Ophthalmological Changes Induced by a 60-Day Head-Down Bed Rest in a Randomized Trial

by Marc Kermorgant, Fanny Varenne, Anne Pavy-Le Traon, Thomas Geeraerts, Lisa Barioulet, Pierre Fournié, Rebecca Billette de Villemeur, Marie-Pierre Bareille, Arnaud Beck, Adrianos Golemis, Inês Antunes, Guillemette Gauquelin-Koch, Vincent Soler and Jean-Claude Quintyn

Life 2024, 14(12), 1598; https://doi.org/10.3390/life14121598 - 4 Dec 2024

Neuro-ophthalmological changes have been reported after prolonged exposure to microgravity; however, the pathophysiology remains unclear. Furthermore, several countermeasures have been suggested to counteract the side effects of microgravity. The objectives of the present study were twofold: (1) to assess the neuro-ophthalmological impact of [...] Read more.

Neuro-ophthalmological changes have been reported after prolonged exposure to microgravity; however, the pathophysiology remains unclear. Furthermore, several countermeasures have been suggested to counteract the side effects of microgravity. The objectives of the present study were twofold: (1) to assess the neuro-ophthalmological impact of 60 days of head-down bed rest (HDBR) and (2) to determine the potential effects of an antioxidant cocktail. In this case, 20 healthy male subjects completed a 60-day HDBR and were randomly allocated into two groups: a control condition without an antioxidant cocktail (CON) and a condition with an antioxidant cocktail (NUT). The retinal nerve fibre layer thickness (RNFLT) and central retinal thickness (CRT) were assessed with spectral domain optical coherence tomography. The optic nerve sheath diameter (ONSD) was measured by ocular ultrasonography and used to assess indirect changes in the intracranial pressure (ICP). The intraocular pressure (IOP) was assessed by Goldmann applanation tonometry. The CRT tended to be reduced after HDBR. The ONSD was increased at the end and after HDBR. The IOP tended to decrease after HDBR. Finally, the antioxidant cocktail had minor impacts on the ophthalmological changes induced by HDBR. It is worth noting that two participants presented peripapillary edema. Full article

(This article belongs to the Special Issue Space Medicine Ophthalmology: Insights from Molecular Observations to the Clinical Management of Ocular Risks in Microgravity)

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12 pages, 4610 KiB

Open AccessArticle

Mitochondrial Genomes of Mammals from the Brazilian Cerrado and Phylogenetic Considerations for the Orders Artiodactyla, Carnivora, and Chiroptera (Chordata: Mammalia)

by Luiz Guilherme Pereira Pimentel, Rafael Augusto Silva Soares, Priscila Martins de Assis, Iuri Batista da Silva, Igor Henrique Rodrigues-Oliveira, Renan Rodrigues Rocha, Vinícius Gonçalves de Miranda, Laiena Luz Bassam, Karine Frehner Kavalco, Fabiano Bezerra Menegídio, Caroline Garcia and Rubens Pasa

Life 2024, 14(12), 1597; https://doi.org/10.3390/life14121597 - 3 Dec 2024

We assembled and annotated the complete mitochondrial genomes of Lycalopex vetulus (hoary fox), Cerdocyon thous (bush dog), Tayassu pecari (white-lipped peccary), and Tadarida brasiliensis (Brazilian free-tailed bat). The mitogenomes exhibited typical vertebrate structures, containing 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA [...] Read more.

We assembled and annotated the complete mitochondrial genomes of Lycalopex vetulus (hoary fox), Cerdocyon thous (bush dog), Tayassu pecari (white-lipped peccary), and Tadarida brasiliensis (Brazilian free-tailed bat). The mitogenomes exhibited typical vertebrate structures, containing 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes, and a D-loop region. Phylogenetic reconstruction using the 13 protein-coding genes revealed robust relationships among species within Carnivora, Chiroptera, and Artiodactyla, corroborating previous studies. Secondary structure analysis of tRNAs and ribosomal genes showed slight variations among species of the same order. This research highlights the importance of mitochondrial genomics in understanding the evolutionary relationships and genetic diversity of Cerrado mammals, contributing to conservation efforts for this unique ecosystem. Full article

(This article belongs to the Section Genetics and Genomics)

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15 pages, 2771 KiB

Open AccessArticle

Utility of Fundus Autofluorescence and Optical Coherence Tomography in Measuring Retinal Vascular Thickness, Macular Density, and Ophthalmic Manifestations in Women with Gestational Diabetes Mellitus

by Rami Al-Dwairi, Omar Altal, Marwa Fares, Sharaf H. Adi, Shahed A. Said, Asmaa Shurair, Rania Al-Bataineh, Ihsan Aljarrah, Seren Al Beiruti, Ahmed H. Al Sharie and Abdelwahab Aleshawi

Life 2024, 14(12), 1596; https://doi.org/10.3390/life14121596 - 3 Dec 2024

Background: Gestational diabetes mellitus (GDM) is a transient elevation of blood glucose during pregnancy. It is typically not associated with diabetic retinopathy. However, certain investigators revealed retinal microvascular injury. In this study, we aimed to assess the ophthalmic findings, optical coherence tomography (OCT) [...] Read more.

Background: Gestational diabetes mellitus (GDM) is a transient elevation of blood glucose during pregnancy. It is typically not associated with diabetic retinopathy. However, certain investigators revealed retinal microvascular injury. In this study, we aimed to assess the ophthalmic findings, optical coherence tomography (OCT) parameters, and retinal vascular thickness and macular density through fundus autofluorescence (FAF). Methods: Prospectively, women diagnosed with GDM were enrolled in this study. All the participants underwent comprehensive ophthalmic examination. Furthermore, macular OCT with analysis of the central subfield thickness (CST) and total thickness was carried out. Moreover, FAF was performed, and the macular density and retinal vascular thickness were extracted using ImageJ software. Results: Thirty-four women were enrolled. The mean maternal age was 32.7 years. No participant had diabetic retinopathy, nine eyes had early cataract, and two eyes had keratoconus. Higher levels for the 1 h oral glucose tolerance test (OGTT) were associated with a drop in the CST and total thickness. Moreover, women who underwent CS had higher levels of total thickness. Higher levels for the fasting OGTT were associated with a thinner inferior temporal retinal artery. Pregnant women with miscarriages had lower macular density on FAF, as represented by lower values of integrated density and mean gray values. Higher levels for the fasting OGTT were associated with higher values of integrated density. Conclusions: Although GDM is typically not associated with diabetic retinopathy, microscopic changes involving the microvascular environment and the macula may occur. Regular ophthalmic screening for women with GDM may be advised. Larger studies with more investigations may reveal further findings. Full article

(This article belongs to the Special Issue Vision Science and Optometry)

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11 pages, 1766 KiB

Open AccessArticle

First Record of Aff. Plesiosuchus sp. (Mesosuchia, Metriorhynchidae) in the Kimmeridgian of Le Havre (Normandy, North-Western France): Biometry, Profile of Locomotion, and Paleobiological Consequences

by Hua Stéphane

Life 2024, 14(12), 1595; https://doi.org/10.3390/life14121595 - 3 Dec 2024

This is the first record of the biggest Metriorhynchidae, aff. Plesiosuchus sp. in France. The remains consist of a partial vertebral column consisting of 11 vertebrae and an ischium fragment. A new method is proposed to evaluate the individual’s size, which is estimated [...] Read more.

This is the first record of the biggest Metriorhynchidae, aff. Plesiosuchus sp. in France. The remains consist of a partial vertebral column consisting of 11 vertebrae and an ischium fragment. A new method is proposed to evaluate the individual’s size, which is estimated at 6.5 m. This method, unlike previous approaches, is based only on vertebrae and yields results that are congruent with those based on cranial remains. The state of preservation has allowed us to test the animal’s ‘profile of locomotion’ to better interpret how it moved. Concerning other metriorhynchids, the record of Dakosaurus in France based only on teeth must be reassessed, and the genus Torvoneustes, if valid, has to be distinguished from Plesiosuchus. Full article

(This article belongs to the Special Issue Back to Basics in Palaeontology)

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11 pages, 2911 KiB

Open AccessArticle

Arthrocentesis of Temporomandibular Joints—A Clinical Comparative Study

by Marta Siewert, Rafał Pokrowiecki, Paweł J. Zawadzki and Zygmunt Stopa

Life 2024, 14(12), 1594; https://doi.org/10.3390/life14121594 - 3 Dec 2024

The objective of this study was to compare single-needle arthrocentesis with the conventional two-needle arthrocentesis, as well as the additional intracapsular injection of hyaluronic acid or platelet-rich fibrin. A total of 96 patients with established osteoarthritis (OA) (n = 48) or with [...] Read more.

The objective of this study was to compare single-needle arthrocentesis with the conventional two-needle arthrocentesis, as well as the additional intracapsular injection of hyaluronic acid or platelet-rich fibrin. A total of 96 patients with established osteoarthritis (OA) (n = 48) or with internal de-arrangement (DD) (n = 48) were assigned single-needle arthrocentesis with distension of the joint or conventional two-needle arthrocentesis with or without intracapsular injection of the medication (hyaluronic acid (HA) or platelet-rich plasma (PRP)) performed every month over a period of 6 months. The maximum mouth opening and pain, as measured by the visual analog scale (VAS), were compared. Each group exhibited significant improvement, i.e., a decrease in pain and an increase in mouth opening. The single-puncture technique provided similar pain reduction as the two-needle approach but provided significantly better results in terms of maximum mouth opening. The reduction in pain was similar when comparing the OA and DD cohorts; however, patients with disc displacement achieved significantly better mouth opening than OA. Intracapsular application of medication contributed to a significant decrease in pain in both HA and PRP groups, with platelet-rich fibrin being significantly superior to HA in terms of mouth opening improvement. Full article

(This article belongs to the Section Medical Research)

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19 pages, 8455 KiB

Open AccessArticle

Eradication of Biofilms on Catheters: Potentials of Tamarix ericoides Rottl. Bark Coating in Preventing Catheter-Associated Urinary Tract Infections (CAUTIs)

by Mohammed H. Karrar Alsharif, Muhammad Musthafa Poyil, Salman Bin Dayel, Mohammed Saad Alqahtani, Ahmed Abdullah Albadrani, Zainab Mohammed M. Omar, Abdullah MR. Arafah, Tarig Gasim Mohamed Alarabi, Reda M. Fayyad and Abd El-Lateef Saeed Abd El-Lateef

Life 2024, 14(12), 1593; https://doi.org/10.3390/life14121593 - 3 Dec 2024

Catheter-associated urinary tract infections (CAUTIs) cause serious complications among hospitalized patients due to biofilm-forming microorganisms which make treatment ineffective by forming antibiotic-resistant strains. As most CAUTI-causing bacterial pathogens have already developed multidrug resistance, there is an urgent need for alternative antibacterial agents to [...] Read more.

Catheter-associated urinary tract infections (CAUTIs) cause serious complications among hospitalized patients due to biofilm-forming microorganisms which make treatment ineffective by forming antibiotic-resistant strains. As most CAUTI-causing bacterial pathogens have already developed multidrug resistance, there is an urgent need for alternative antibacterial agents to prevent biofilms on catheter surfaces. As a trial to find out such a potential agent of natural origin, the bark of Tamarix ericoides Rottl., a little-known plant from the Tamaricaceae family, was examined for its antibacterial and antibiofilm activities against one of the major, virulent, CAUTI-causing bacterial pathogens: Enterococcus faecalis. The methanolic T. ericoides bark extract was analyzed for its antibacterial activity using the well diffusion method and microdilution method. Killing kinetics were calculated using time–kill assay, and the ability of biofilm formation and its eradication upon treatment with the T. ericoides bark extract was studied by crystal violet assay. GC-MS analysis was performed to understand the phytochemical presence in the extract. A in vitro bladder model study was performed using extract-coated catheters against E. faecalis, and the effect was visualized using CLSM. The changes in the cell morphology of the bacterium after treatment with the T. ericoides bark extract were observed using SEM. The biocompatibility of the extract towards L₉₂₉ cells was studied by MTT assay. The anti-E. faecalis activity of the extract-coated catheter tube was quantified by viable cell count method, which exposed 20% of growth after five days of contact with E. faecalis. The anti-adhesive property of the T. ericoides bark extract was studied using CLSM. The extract showed potential antibacterial activity, and the lowest inhibitory concentration needed to inhibit the growth of E. faecalis was found to be 2 mg/mL. The GC-MS analysis of the methanolic fractions of the T. ericoides bark extract revealed the presence of major phytochemicals, such as diethyl phthalate, pentadecanoic acid, methyl 6,11-octadecadienoate, cyclopropaneoctanoic acid, 2-[(2-pentylcyclopropyl) methyl]-, methyl ester, erythro-7,8-bromochlorodisparlure, etc., that could be responsible for the antibacterial activity against E. faecalis. The killing kinetics of the extract against E. faecalis was calculated and the extract showed promising antibiofilm activity on polystyrene surfaces. The T. ericoides bark extract effectively reduced the E. faecalis mature biofilms by 75%, 82%, and 83% after treatment with 1X MIC (2 mg/mL), 2X MIC (4 mg/mL), and 3X MIC (6 mg/mL) concentrations, respectively, which was further confirmed by SEM analysis. The anti-adhesive property of the T. ericoides bark extract studied using CLSM revealed a reduction in the biofilm thickness, and the FDA and PI combination revealed the death of 80% of the cells on the extract-coated catheter tube. In addition, SEM analysis showed extensive damage to the E. faecalis cells after the T. ericoides bark extract treatment, and it was not cytotoxic. Hence, after further studies, T. ericoides bark extract with potential antibacterial, antibiofilm, and anti-adhesive activities can be developed as an alternative agent for treating CAUTIs. Full article

(This article belongs to the Special Issue Synthesis and Evaluation of Novel Anticancer and Anti-Inflammatory Agents)

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16 pages, 812 KiB

Open AccessReview

Mechanisms of Cbl-Mediated Ubiquitination of Proteins in T and Natural Killer Cells and Effects on Immune Cell Functions

by Pulak Ranjan Nath and Noah Isakov

Life 2024, 14(12), 1592; https://doi.org/10.3390/life14121592 - 3 Dec 2024

Post-translational ubiquitination is an essential mechanism for the regulation of protein stability and function, which contributes to the regulation of the immune system. Cbl, an E3 ubiquitin ligase, is particularly well-characterized in the context of T and NK cell signaling, where it serves [...] Read more.

Post-translational ubiquitination is an essential mechanism for the regulation of protein stability and function, which contributes to the regulation of the immune system. Cbl, an E3 ubiquitin ligase, is particularly well-characterized in the context of T and NK cell signaling, where it serves as a key regulator of receptor downstream signaling events and as a modulator of cell activation. Cbl promotes the proteasomal degradation of TCR/CD3 subunits as well as the protein kinases Fyn and Lck in T cells. Additionally, the scaffold protein linker for activation of T cells (LAT) is a universal target for Cbl-mediated ubiquitination and degradation in both T and NK cells. Recent findings suggest that CrkII-mediated ubiquitination and degradation of C3G by Cbl during early T cell activation may also be relevant to NK cell signaling. Given its role in modulating immune responses and its manageable impact on autoimmunity, Cbl is being investigated as a target for cancer immunotherapy. This review explores the ubiquitin ligase activity of Cbl and its implications for CAR T and NK cell immunotherapies. Full article

(This article belongs to the Collection Feature Review Papers for Life)

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13 pages, 2471 KiB

Open AccessArticle

Association Between Severe Periodontitis and Cognitive Decline in Older Adults

by Yash Brahmbhatt, Hend Alqaderi and Zahra Chinipardaz

Life 2024, 14(12), 1589; https://doi.org/10.3390/life14121589 - 3 Dec 2024

(1) Background: Periodontal disease, a progressive inflammatory condition, disrupts the oral microbiome and releases inflammatory cytokines, leading to systemic issues, including cognitive decline. This study investigates the association between severe periodontitis and cognitive decline, exploring the role of alkaline phosphatase (ALP), an enzyme [...] Read more.

(1) Background: Periodontal disease, a progressive inflammatory condition, disrupts the oral microbiome and releases inflammatory cytokines, leading to systemic issues, including cognitive decline. This study investigates the association between severe periodontitis and cognitive decline, exploring the role of alkaline phosphatase (ALP), an enzyme linked to systemic inflammation, as an effect modifier. (2) Methods: We analyzed cross-sectional data from the 2013–2014 National Health and Nutrition Examination Survey (NHANES). Severe periodontitis was defined using the Centers for Disease Control and Prevention (CDC) and the American Academy of Pediatrics (AAP) case definition. A weighted multivariable logistic regression model assessed the association between severe periodontitis and cognitive decline. An interaction term examined ALP’s role as an effect modifier. (3) Results: This study included 1265 participants aged 65 and older. After adjusting for confounders, each one-point increase in cognitive function score was associated with a 2% decrease in the odds of severe periodontitis (OR = 0.98; 95% CI = 0.97–0.99; p = 0.008). ALP was a significant effect modifier in the relationship between severe periodontitis and cognitive decline. (4) Conclusions: This study, using a representative U.S. adult population aged 65 and over, suggests that lower cognitive performance correlates with higher likelihood of severe periodontitis. ALP enhances the association between severe periodontitis and cognitive decline. Full article

(This article belongs to the Section Physiology and Pathology)

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16 pages, 4485 KiB

Open AccessArticle

RtHSFA9s of Rhodomyrtus tomentosa Positively Regulate Thermotolerance by Transcriptionally Activating RtHSFA2s and RtHSPs

by Huiguang Li, Ling Yang, Yujie Fang, Gui Wang and Tingting Liu

Life 2024, 14(12), 1591; https://doi.org/10.3390/life14121591 - 2 Dec 2024

Heat shock transcription factors (HSFs) are crucial components in heat stress response. However, the contribution of the HSFs governing the inherent thermotolerance in Rhodomyrtus tomentosa has barely been investigated. We here compared the roles of RtHSFA9a, RtHSFA9b, and RtHSFA9c in heat [...] Read more.

Heat shock transcription factors (HSFs) are crucial components in heat stress response. However, the contribution of the HSFs governing the inherent thermotolerance in Rhodomyrtus tomentosa has barely been investigated. We here compared the roles of RtHSFA9a, RtHSFA9b, and RtHSFA9c in heat stress tolerance. These three genes are the results of gene duplication events, but there exist vast variations in their amino acid sequences. They are all localized to the nucleus. Arabidopsis thaliana plants with overexpressed RtHSFA9a and RtHSFA9c outperformed the wild-type plants, while the over-accumulation of RtHSFA9b had little impact on plant thermotolerance. By transiently overexpressing RtHSFA9a, RtHSFA9b, and RtHSFA9c in R. tomentosa seedlings, the mRNA abundance of heat shock response genes, including RtHSFA2a, RtHSFA2b, RtHSP17.4, RtHSP21.8, RtHSP26.5, and RtHSP70, were upregulated. Transactivation assays confirmed that there exist regulatory divergences among these three genes, viz., RtHSFA9a has the highest transcription activity in regulating RtHSFA2a, RtHSFA2b, RtHSP21.8, and RtHSP70; RtHSFA9c can transcriptionally activate RtHSFA2b, RtHSP21.8, and RtHSP70; RtHSFA9b makes limited contributions to the accumulation of RtHSFA2b, RtHSP21.8, and RtHSP70. Our results indicate that the RtHSFA9 genes make crucial contributions to the thermal adaption of R. tomentosa by positively regulating the RtHSFA2a, RtHSFA2b, and RtHSP genes, which provides novel insights into the RtHSFA9 subfamily. Full article

(This article belongs to the Special Issue Plant Biotic and Abiotic Stresses 2024)

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9 pages, 238 KiB

Open AccessReview

Arrhythmias Following Patent Foramen Ovale Closure: An Unsolved Enigma

by Aikaterini-Eleftheria Karanikola, Stergios Soulaidopoulos, Ioannis Leontsinis, Eirini Dri, Marios Sagris, Athanasios Kordalis, Konstantinos Aznaouridis, Dimitrios Tsiachris and Konstantinos Tsioufis

Life 2024, 14(12), 1590; https://doi.org/10.3390/life14121590 - 2 Dec 2024

Patent foramen ovale (PFO) closure has proven to be an effective method of reducing the risk of recurrent stroke in patients with embolic stroke of unknown origin (ESUS). One of the most recognized post-procedural complications is the de novo occurrence of supraventricular arrhythmias, [...] Read more.

Patent foramen ovale (PFO) closure has proven to be an effective method of reducing the risk of recurrent stroke in patients with embolic stroke of unknown origin (ESUS). One of the most recognized post-procedural complications is the de novo occurrence of supraventricular arrhythmias, mainly atrial fibrillation, in the first three months following PFO closure. Earlier studies reported the incidence to be around 3.4–7%; however, this percentage has risen in recent studies up to 21%. The pathogenesis behind this type of arrhythmia is complex and not clearly understood, although it seems that direct effects of the device on the atria, as well as an inflammatory response, are the two most prevalent mechanisms. Management of this complication might be challenging given the heterogenicity of patient characteristics, so an individualized approach is most wisely followed. This review aims to present the current data on the incidence, pathogenesis and therapeutic strategies behind this rather common concern in an era of increasing transcatheter interventions for PFO. Full article

(This article belongs to the Special Issue Cardiac Arrhythmias: Physiological Mechanisms, Clinical Insights, and Epidemiological Research)

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11 pages, 590 KiB

Open AccessArticle

Evaluation of Ocular and Systemic Oxidative Stress Markers in Patients with Diabetic Retinopathy

by Ana Karen López-Contreras, Diana Esperanza Arévalo-Simental, Fermín Paúl Pacheco-Moisés, María Guadalupe Martínez-Ruíz, Cecilia Olvera-Montaño, Ricardo Raúl Robles-Rivera, Sonia Sifuentes-Franco, Tannia Isabel Campos-Bayardo, Selene Guadalupe Huerta-Olvera and Adolfo Daniel Rodríguez-Carrizalez

Life 2024, 14(12), 1588; https://doi.org/10.3390/life14121588 - 2 Dec 2024

Proliferative diabetic retinopathy (PDR) is the most severe complication of chronic hyperglycaemi stimulates oxidative stress that changes the retinal basement membrane function and provokes neovascularization, macular edema and retinal detachment. But an oxidative–antioxidant biomarker assessment in ocular matrices, such as aqueous humor (AH) [...] Read more.

Proliferative diabetic retinopathy (PDR) is the most severe complication of chronic hyperglycaemi stimulates oxidative stress that changes the retinal basement membrane function and provokes neovascularization, macular edema and retinal detachment. But an oxidative–antioxidant biomarker assessment in ocular matrices, such as aqueous humor (AH) and vitreous, might show the oxidative stress (OS) status in the posterior segment. Here, we show a cross-sectional analytical study of 39 patients who had a vitrectomy and assess the levels of different oxidative–antioxidant biomarkers in blood, aqueous and vitreous humor in three groups: diabetes mellitus 2 (DM2) with PDR [DM(+)PDR(+)] (n =13), DM2 without PDR [DM(+)PDR(−)] (n = 13) and non-DM2 non-PDR [DM(−)PDR(−)] as the control group (n = 13). Our finding suggests the presence of oxidative stress in diabetic retinopathy, as evidenced by increased levels of 8-isoprostanes and decreased levels of total antioxidant capacity from stages before the development of diabetic retinopathy. Our results reveal a notable increment in catalase levels in the DM(+)PDR(+) group in blood and vitreous humor. Likewise, we identified that the DM(+)PDR(−) group presents significant levels in 8-IP and SOD in vitreous humor and blood versus aqueous humor. These finding suggest the role of antioxidant enzymes in compensating oxidative stress mechanisms in PDR development. Full article

(This article belongs to the Special Issue Eye Diseases: Diagnosis and Treatment, 2nd Edition)

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14 pages, 787 KiB

Open AccessArticle

Soluble P-Selectin as an Indicator of Cutaneous Microangiopathy in Uncomplicated Young Patients with Type 1 Diabetes

by Jolanta Neubauer-Geryk, Małgorzata Myśliwiec, Katarzyna Zorena and Leszek Bieniaszewski

Life 2024, 14(12), 1587; https://doi.org/10.3390/life14121587 - 2 Dec 2024

This study aimed to analyze the relationship between cutaneous microcirculation reactivity, retinal circulation, macrocirculation function, and specific adhesion molecules in young patients with uncomplicated type 1 diabetes. Fifty-five patients with type 1 diabetes mellitus (T1DM), aged 8 to 18 years, were divided into [...] Read more.

This study aimed to analyze the relationship between cutaneous microcirculation reactivity, retinal circulation, macrocirculation function, and specific adhesion molecules in young patients with uncomplicated type 1 diabetes. Fifty-five patients with type 1 diabetes mellitus (T1DM), aged 8 to 18 years, were divided into subgroups based on skin microcirculation reactivity. The cutaneous microcirculatory vessels were considered reactive if post-test PORH coverage increased compared to pre-test coverage. Optical coherence tomography (OCT) was conducted to detect early retinopathic changes. Macrocirculation was described using pulsatility indices (PIs) determined for common carotid (CCA) and peripheral arteries of the upper and lower limbs. The ankle–brachial index was also assessed. There were no significant differences in retinal circulation and macrocirculation between the studied subgroups. However, there were significant differences between the various subgroups concerning the age at onset of diabetes and the sP-selectin levels but not ICAM-1 and sVCAM-1. The sP-selectin differences remained true after adjusting for age at onset. The sP-selectin level was significantly higher in the subgroup of patients with non-reactive cutaneous microcirculation. The results of our study indicate that sP-selectin may be considered as an immunological marker for cutaneous abnormalities, which serve as an early indicator of endothelial dysfunction in young patients with type 1 diabetes in the absence of classical complication. Full article

(This article belongs to the Special Issue Management of Patients with Diabetes)

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