Life

19 pages, 2442 KiB

Open AccessReview

Advances and Mechanisms of RNA–Ligand Interaction Predictions

by Chen Zhuo, Chengwei Zeng, Haoquan Liu, Huiwen Wang, Yunhui Peng and Yunjie Zhao

Life 2025, 15(1), 104; https://doi.org/10.3390/life15010104 - 15 Jan 2025

The diversity and complexity of RNA include sequence, secondary structure, and tertiary structure characteristics. These elements are crucial for RNA’s specific recognition of other molecules. With advancements in biotechnology, RNA–ligand structures allow researchers to utilize experimental data to uncover the mechanisms of complex [...] Read more.

The diversity and complexity of RNA include sequence, secondary structure, and tertiary structure characteristics. These elements are crucial for RNA’s specific recognition of other molecules. With advancements in biotechnology, RNA–ligand structures allow researchers to utilize experimental data to uncover the mechanisms of complex interactions. However, determining the structures of these complexes experimentally can be technically challenging and often results in low-resolution data. Many machine learning computational approaches have recently emerged to learn multiscale-level RNA features to predict the interactions. Predicting interactions remains an unexplored area. Therefore, studying RNA–ligand interactions is essential for understanding biological processes. In this review, we analyze the interaction characteristics of RNA–ligand complexes by examining RNA’s sequence, secondary structure, and tertiary structure. Our goal is to clarify how RNA specifically recognizes ligands. Additionally, we systematically discuss advancements in computational methods for predicting interactions and to guide future research directions. We aim to inspire the creation of more reliable RNA–ligand interaction prediction tools. Full article

(This article belongs to the Section Genetics and Genomics)

13 pages, 847 KiB

Open AccessArticle

The Effect of Audible Joint Manipulation Sounds in the Upper Cervical Spine on Brain Wave and Autonomic Nervous System Activity

by Dalton Whitman, Rob Sillevis and Matthew Frommelt

Life 2025, 15(1), 103; https://doi.org/10.3390/life15010103 - 15 Jan 2025

Abstract

Background: High-velocity, low-amplitude (HVLA) manipulation is a common manual therapy technique used for treating pain and musculoskeletal dysfunction. An audible manipulation sound is commonly experienced by patients who undergo HVLA manipulation; however, there is little known about the effects and clinical relevance of [...] Read more.

Background: High-velocity, low-amplitude (HVLA) manipulation is a common manual therapy technique used for treating pain and musculoskeletal dysfunction. An audible manipulation sound is commonly experienced by patients who undergo HVLA manipulation; however, there is little known about the effects and clinical relevance of the audible manipulation sound on cortical output and the autonomic nervous system. This study aimed to identify the immediate impact of the audible manipulation sound on brainwave activity and pupil diameter in asymptomatic subjects following an HVLA cervical manipulation. Methods: 40 subjects completed this quasi-experimental repeated measure study design. Subjects were connected to electroencephalography and pupillometry simultaneously, and an HVLA cervical distraction manipulation was performed. The testing environment was controlled to optimize brainwave and pupillometry data acquisition. Pre-manipulation, immediately after manipulation, and post-manipulation data were collected. The presence of an audible manipulation sound was noted. Results: Twenty subjects experienced an audible manipulation sound. Brainwave activity changes were significant (p < 0.05) in both the audible manipulation sound and non-manipulation sound groups. Pupil diameter changes (p < 0.05) occurred in both eyes of the non-manipulation sound group and in the left eye of the audible-manipulation sound group. Brainwave activity patterns were similar in both groups. Conclusions: The presence of an audible manipulation sound is not required to produce central nervous system changes following an HVLA cervical manipulation; however, the audible manipulation sound does prolong the effects of brainwave activity, indicating a prolonged relaxation effect. Full article

(This article belongs to the Special Issue Advances in Non-Invasive Brain Stimulation)

22 pages, 3383 KiB

Open AccessArticle

Complete Sequence and Characterization of Mitochondrial and Chloroplast Genome of Navicula incerta CACC 0356

by Zhaokai Wang and Xiaoyu Wei

Life 2025, 15(1), 102; https://doi.org/10.3390/life15010102 - 15 Jan 2025

Abstract

Navicula incerta, a marine benthic diatom, holds promise for human nutrition and health as well as for aquaculture applications. However, the scarcity of organelle genome data within the Navicula clade has impeded a comprehensive understanding and utilization of this group. Our research [...] Read more.

Navicula incerta, a marine benthic diatom, holds promise for human nutrition and health as well as for aquaculture applications. However, the scarcity of organelle genome data within the Navicula clade has impeded a comprehensive understanding and utilization of this group. Our research presents a pioneering exploration into the complete mitochondrial and chloroplast genome sequences of N. incerta CACC 0356, shedding light on its phylogeny and evolutionary history. The mitochondrial genome (mtDNA) spans 61,320 bp with a GC content of 29.87%, comprising one circular DNA molecule that encodes for 34 protein-coding genes, 24 tRNAs, and 34 rRNAs. Whereas, the chloroplast genome (cpDNA) is larger at 129,090 bp, encompassing 127 protein-coding genes, 30 tRNAs, and 7 rRNAs. Notably, the cpDNA of N. incerta is approximately 2.1 times the size of its mtDNA. Our annotation identified four genes that are partially situated in the homologous regions between the mitogenome and chloroplast genome, totaling 372 bp, which represents 0.61% of the entire mitogenome. Comparative analyses revealed that N. incerta CACC 0356 is closely affiliated with Fistulifera saprophila and Fistulifera solaris, both members of the Naviculaceae order. This study significantly expands the mitochondrial and chloroplast genomic resources for algae and lays a foundation for the development of genetic diversity analyses in algae. Full article

(This article belongs to the Section Genetics and Genomics)

24 pages, 583 KiB

Open AccessReview

Unlocking the Potential of Cecostomies: A Valuable Lifesaving Procedure in Emergency Surgery for Colonic Obstructions

by Constantin Popazu, Alexandra Toma, Daniela Mihalache, Oana-Monica Duca, Dorel Firescu and Dragoș F. Voicu

Life 2025, 15(1), 101; https://doi.org/10.3390/life15010101 - 15 Jan 2025

Abstract

Background: Colonic obstructions present a serious medical emergency that requires prompt surgical intervention to prevent life-threatening complications. Cecostomy, a procedure involving the creation of an opening in the cecum to decompress the colon, serves as one surgical approach for managing these obstructions. The [...] Read more.

Background: Colonic obstructions present a serious medical emergency that requires prompt surgical intervention to prevent life-threatening complications. Cecostomy, a procedure involving the creation of an opening in the cecum to decompress the colon, serves as one surgical approach for managing these obstructions. The aim of this review is to evaluate the effectiveness and benefits of cecostomies in emergency surgical settings, with a focus on recent clinical studies and case reports. Cecostomy is highlighted as a bridge procedure in cases such as obstructive carcinomas, providing data on success rates, relative survival, and clinical effectiveness. The importance of the patient’s condition and surgeon expertise in selecting cecostomy as a procedure is emphasized. Further comparative research is suggested to optimize the selection criteria, providing a strong, clinically oriented conclusion. Methods: A comprehensive literature review was conducted to identify studies and case reports focusing on the application of cecostomies in cases of acute colonic obstruction. Articles were selected based on their relevance to emergency surgery, the effectiveness of cecostomies, and patient outcomes in various clinical scenarios, including obstructive carcinomas and colonic pseudo-obstructions. Results: The analysis reveals that cecostomies provide rapid decompression and effective relief from colonic obstruction, particularly when immediate intervention is needed to prevent bowel perforation or ischemia. In several cases, cecostomies act as a bridge to more definitive surgical treatments, such as resection and anastomosis, and are associated with reduced morbidity and mortality. The selection of cecostomy as a preferred procedure depends on the patient’s condition, location of the obstruction, and surgeon expertise. Conclusions: Cecostomies play a crucial role in the emergency management of colonic obstructions, offering a viable and sometimes lifesaving alternative for rapid decompression. Understanding the indications and appropriate use of cecostomies can enhance patient outcomes and provide surgeons with effective strategies for managing acute colonic obstructions. Further research is warranted to refine selection criteria and to compare outcomes between cecostomies and other decompressive techniques in emergency settings. Full article

(This article belongs to the Section Medical Research)

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22 pages, 10360 KiB

Open AccessArticle

Conformational Dynamics of Mitochondrial Inorganic Pyrophosphatase hPPA2 and Its Changes Caused by Pathogenic Mutations

by Ekaterina Bezpalaya, Svetlana Kurilova, Nataliya Vorobyeva and Elena Rodina

Life 2025, 15(1), 100; https://doi.org/10.3390/life15010100 - 15 Jan 2025

Abstract

Inorganic pyrophosphatases, or PPases, are ubiquitous enzymes whose activity is necessary for a large number of biosynthetic reactions. The catalytic function of PPases is dependent on certain conformational changes that have been previously characterized based on the comparison of the crystal structures of [...] Read more.

Inorganic pyrophosphatases, or PPases, are ubiquitous enzymes whose activity is necessary for a large number of biosynthetic reactions. The catalytic function of PPases is dependent on certain conformational changes that have been previously characterized based on the comparison of the crystal structures of various complexes. The current work describes the conformational dynamics of a structural model of human mitochondrial pyrophosphatase hPPA2 using molecular dynamics simulation, all-atom principal component analysis, and coarse-grained normal mode analysis. In addition to the wild-type enzyme, four mutant variants of hPPA2 were characterized that correspond to the natural pathogenic variants causing severe mitochondrial dysfunction and cardio pathologies. As a result, we identified the global type of flexible motion that seems to be shared by other dimeric PPases. This motion is discussed in terms of the allosteric behavior of the protein. Analysis of the observed conformational dynamics revealed the formation of a binding site for anionic ligands in the active site that could be relevant to enzyme catalysis. Based on the comparison of the wild-type and mutant PPases dynamics, we suggest the possible molecular mechanisms of the functional incompetence of hPPA2 caused by mutations. The results of this work allow for deeper insight into the structural basis of PPase function and the possible effects of pathogenic mutations on the protein structure and function. Full article

(This article belongs to the Special Issue Applications of Molecular Dynamics to Biological Systems)

13 pages, 3630 KiB

Open AccessArticle

NGF-TrkA Axis Enhances PDGF-C-Mediated Angiogenesis in Osteosarcoma via miR-29b-3p Suppression: A Potential Therapeutic Strategy Using Larotrectinib

by Sheng-Mou Hou, Ching-Yuan Cheng, Wei-Li Chen, En-Ming Chang and Chih-Yang Lin

Life 2025, 15(1), 99; https://doi.org/10.3390/life15010099 - 15 Jan 2025

Abstract

Angiogenesis plays a critical role in osteosarcoma (OS) growth and metastasis. While nerve growth factor (NGF) is implicated in cancer progression, its role in OS angiogenesis remains unclear. This study explored NGF’s effects on angiogenesis and the underlying molecular mechanisms. Analysis of GEO [...] Read more.

Angiogenesis plays a critical role in osteosarcoma (OS) growth and metastasis. While nerve growth factor (NGF) is implicated in cancer progression, its role in OS angiogenesis remains unclear. This study explored NGF’s effects on angiogenesis and the underlying molecular mechanisms. Analysis of GEO (GSE16088) data identified five angiogenesis markers significantly upregulated in OS tissues. In vitro experiments demonstrated that NGF enhanced HUVEC tube formation by upregulating platelet-derived growth factor C (PDGF-C) expression and suppressing microRNA-29b-3p (miR-29b-3p). The results of tube formation assays confirmed that NGF stimulation significantly increased the angiogenic capacity of MG63/NGF cells compared to MG63 cells. Furthermore, larotrectinib, a TrkA inhibitor, effectively reduced the migration and invasion abilities of MG63/NGF cells in a dose-dependent manner. These findings suggest that the NGF-TrkA axis promotes PDGF-C-mediated angiogenesis by inhibiting miR-29b-3p signaling. Larotrectinib could serve as a potential therapeutic agent targeting NGF-mediated angiogenesis in OS, offering a promising avenue for treatment. Full article

(This article belongs to the Special Issue Bone Cancer: From Molecular Mechanism to Treatment)

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13 pages, 9125 KiB

Open AccessArticle

Influence of Environmental Factors on the Aboveground Biomass of Mature and Postmining Forests in Chocó

by David Pérez Abadía, Harley Quinto Mosquera and José María Del Arco

Life 2025, 15(1), 98; https://doi.org/10.3390/life15010098 - 15 Jan 2025

Abstract

Environmental factors control the accumulation of aboveground biomass (AB) in tropical forests, along with the role of AB in climate change mitigation. As such, the objective of this study was to evaluate the influence of factors such as forest type, succession, abundance of [...] Read more.

Environmental factors control the accumulation of aboveground biomass (AB) in tropical forests, along with the role of AB in climate change mitigation. As such, the objective of this study was to evaluate the influence of factors such as forest type, succession, abundance of individuals, species richness, height, diameter, texture, and soil nutrient levels on the AB in mature and postmining forests in Chocó, Colombia. Five plots each were set up in primary and postmining forests with 15 and 30 years of regeneration, in which the amount of AB was measured and related to the environmental factors. The AB was 178.32 t ha⁻¹ in the mature forests and 35.17 and 56.3 t ha⁻¹ after 15 and 30 years of postmining regeneration, respectively. Furthermore, the general AB level was determined by the ecosystem type, diameter, richness, abundance, Shannon evenness, and Margalef diversity. In mature forests, the AB amount was positively influenced by height and richness, and negatively influenced by dominance and evenness; in areas degraded by mining, the AB level was positively related to richness and equity, and negatively related to species diversity and soil silt. In summary, environmental factors determine the carbon storage in the forests in Chocó. Mining reduces the function of these ecosystems in mitigating climate change. Full article

(This article belongs to the Special Issue Carbon and Nitrogen Cycles in Terrestrial Ecosystems)

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15 pages, 943 KiB

Open AccessArticle

Laparoscopic and Open Distal Pancreatectomy—An Initial Single-Institution Experience with a Propensity Score Matching Analysis

by Irena Plahuta, Žan Šarenac, Medeja Golob, Špela Turk, Bojan Ilijevec, Tomislav Magdalenić, Stojan Potrč and Arpad Ivanecz

Life 2025, 15(1), 97; https://doi.org/10.3390/life15010097 - 14 Jan 2025

Abstract

Laparoscopic distal pancreatectomy is a minimally invasive approach for the surgical treatment of neoplasms in the distal pancreas. This study aimed to compare this approach to the open procedure. A retrospective analysis of a prospectively maintained database of 400 pancreatectomies was performed. The [...] Read more.

Laparoscopic distal pancreatectomy is a minimally invasive approach for the surgical treatment of neoplasms in the distal pancreas. This study aimed to compare this approach to the open procedure. A retrospective analysis of a prospectively maintained database of 400 pancreatectomies was performed. The laparoscopic distal pancreatectomy group (LDP) was compared to the open distal pancreatectomy group (ODP). A propensity score matching analysis (PSM) was performed. From 2016 to 2023, 108 distal pancreatectomies were carried out, 19 (17.6%) laparoscopically and 89 (82.4%) openly. The conversion rate was 13.6%. The severe morbidity rates were 28.1% in the ODP group, 47.4% in the LDP group, and 15.8% in the ODP-PSM group. The difference between the latter two was statistically significant (p = 0.034) due to the high rate of Clavien–Dindo grade 3a complications (42.1% versus 10.5%, p = 0.042) in the LDP group. The 90-day mortality rates were 3.3% in the ODP group and 5.3% in the other two groups. The LDP group had a shorter duration of intravenous narcotic analgesia (5 versus 7 days, p = 0.041). There was no difference in the R0 resection or postoperative pancreatic fistula rates. Our attention should be drawn to preventing postoperative complications because the oncological outcomes are already comparable with those of the open procedure, and postoperative pain management is promising. Full article

(This article belongs to the Special Issue Gastrointestinal Health: Clinical Research and Therapeutic Innovations)

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27 pages, 856 KiB

Open AccessReview

Alzheimer’s Disease and Porphyromonas gingivalis: Exploring the Links

by Ivana Shawkatova, Vladimira Durmanova and Juraj Javor

Life 2025, 15(1), 96; https://doi.org/10.3390/life15010096 - 14 Jan 2025

Abstract

Recent research highlights compelling links between oral health, particularly periodontitis, and systemic diseases, including Alzheimer’s disease (AD). Although the biological mechanisms underlying these associations remain unclear, the role of periodontal pathogens, particularly Porphyromonas gingivalis, has garnered significant attention. P. gingivalis, a [...] Read more.

Recent research highlights compelling links between oral health, particularly periodontitis, and systemic diseases, including Alzheimer’s disease (AD). Although the biological mechanisms underlying these associations remain unclear, the role of periodontal pathogens, particularly Porphyromonas gingivalis, has garnered significant attention. P. gingivalis, a major driver of periodontitis, is recognized for its potential systemic effects and its putative role in AD pathogenesis. This review examines evidence connecting P. gingivalis to hallmark AD features, such as amyloid β accumulation, tau hyperphosphorylation, neuroinflammation, and other neuropathological features consistent with AD. Virulence factors, such as gingipains and lipopolysaccharides, were shown to be implicated in blood–brain barrier disruption, neuroinflammation, and neuronal damage. P. gingivalis-derived outer membrane vesicles may serve to disseminate virulence factors to brain tissues. Indirect mechanisms, including systemic inflammation triggered by chronic periodontal infections, are also supposed to exacerbate neurodegenerative processes. While the exact pathways remain uncertain, studies detecting P. gingivalis virulence factors and its other components in AD-affected brains support their possible role in disease pathogenesis. This review underscores the need for further investigation into P. gingivalis-mediated mechanisms and their interplay with host responses. Understanding these interactions could provide critical insights into novel strategies for reducing AD risk through periodontal disease management. Full article

(This article belongs to the Special Issue Alzheimer’s Disease: Recent Developments in Pathogenesis, Diagnosis, and Therapy)

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16 pages, 2990 KiB

Open AccessArticle

A Novel Approach for In Vitro Testing and Hazard Evaluation of Nanoformulated RyR2-Targeting siRNA Drugs Using Human PBMCs

by Valeria Bettinsoli, Gloria Melzi, Angelica Crea, Lorenzo Degli Esposti, Michele Iafisco, Daniele Catalucci, Paolo Ciana and Emanuela Corsini

Life 2025, 15(1), 95; https://doi.org/10.3390/life15010095 - 14 Jan 2025

Abstract

Nucleic acid (NA)-based drugs are promising therapeutics agents. Beyond efficacy, addressing safety concerns—particularly those specific to this class of drugs—is crucial. Here, we propose an in vitro approach to screen for potential adverse off-target effects of NA-based drugs. Human peripheral blood mononuclear cells [...] Read more.

Nucleic acid (NA)-based drugs are promising therapeutics agents. Beyond efficacy, addressing safety concerns—particularly those specific to this class of drugs—is crucial. Here, we propose an in vitro approach to screen for potential adverse off-target effects of NA-based drugs. Human peripheral blood mononuclear cells (PBMCs), purified from buffy coats of healthy donors, were used to investigate the ability of NA-drugs to trigger toxicity pathways and inappropriate immune stimulation. PBMCs were selected for their ability to represent potential human responses, given their likelihood of interacting with administered drugs. As proof of concept, a small interfering RNA (siRNA) targeting Ryanodine Receptor mRNA (RyR2) identified by the Italian National Center for Gene Therapy and Drugs based on RNA Technology as a potential therapeutic target for dominant catecholaminergic polymorphic ventricular tachycardia, was selected. This compound and its scramble were formulated within a calcium phosphate nanoparticle-based delivery system. Positive controls for four toxicity pathways were identified through literature review, each associated with a specific type of cellular stress: oxidative stress (tert-butyl hydroperoxide), mitochondrial stress (rotenone), endoplasmic reticulum stress (thapsigargin), and autophagy (rapamycin). These controls were used to define specific mRNA signatures triggered in PBMCs, which were subsequently used as indicators of off-target effects. To assess immune activation, the release of pro-inflammatory cytokines (interleukin-6, interleukin-8, tumor necrosis factor-α, and interferon-γ) was measured 24 h after exposure. The proposed approach provides a rapid and effective screening method for identifying potential unintended effects in a relevant human model, which also allows to address gender effects and variability in responses. Full article

(This article belongs to the Section Pharmaceutical Science)

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20 pages, 260 KiB

Open AccessReview

Transforming Cardiovascular Risk Prediction: A Review of Machine Learning and Artificial Intelligence Innovations

by Dimitrios-Ioannis Kasartzian and Thomas Tsiampalis

Life 2025, 15(1), 94; https://doi.org/10.3390/life15010094 - 14 Jan 2025

Abstract

Cardiovascular diseases (CVDs) remain a leading cause of global mortality and morbidity. Traditional risk prediction models, while foundational, often fail to capture the multifaceted nature of risk factors or leverage the expanding pool of healthcare data. Machine learning (ML) and artificial intelligence (AI) [...] Read more.

Cardiovascular diseases (CVDs) remain a leading cause of global mortality and morbidity. Traditional risk prediction models, while foundational, often fail to capture the multifaceted nature of risk factors or leverage the expanding pool of healthcare data. Machine learning (ML) and artificial intelligence (AI) approaches represent a paradigm shift in risk prediction, offering dynamic, scalable solutions that integrate diverse data types. This review examines advancements in AI/ML for CVD risk prediction, analyzing their strengths, limitations, and the challenges associated with their clinical integration. Recommendations for standardization, validation, and future research directions are provided to unlock the potential of these technologies in transforming precision cardiovascular medicine. Full article

(This article belongs to the Special Issue Advancements in Cardiovascular Epidemiology: Integrating Predictive Modeling into Public Health)

12 pages, 564 KiB

Open AccessReview

Advances in the Study of Heartwood Formation in Trees

by Shuqi Yang, Fangcuo Qin, Shengkun Wang, Xiang Li, Yunqing Zhou and Sen Meng

Life 2025, 15(1), 93; https://doi.org/10.3390/life15010093 - 14 Jan 2025

Abstract

Heartwood, serving as the central constituent of the xylem, plays a crucial role in the growth, development, and resilience of trees. The process of heartwood formation constitutes a complex biological phenomenon influenced by various factors. A thorough examination of the mechanisms underpinning heartwood [...] Read more.

Heartwood, serving as the central constituent of the xylem, plays a crucial role in the growth, development, and resilience of trees. The process of heartwood formation constitutes a complex biological phenomenon influenced by various factors. A thorough examination of the mechanisms underpinning heartwood formation not only enhances our understanding of the growth and developmental paradigms regulating trees but also provides essential theoretical support and practical insights for the timber industry, forestry management, and ecological conservation. This paper offers an overview of the foundational processes involved in heartwood formation in plants. Furthermore, it presents a comprehensive review of the latest research advancements in this domain, covering five key aspects: metabolism, hormonal regulation, transcriptional regulation, cell biology, and environmental influences. This review serves as a valuable basis for future research endeavors in related academic fields. Full article

(This article belongs to the Special Issue Plant Physiology: From Omic Analysis Toward Physiological Mechanism Research: 2nd Edition)

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19 pages, 2477 KiB

Open AccessArticle

The Five-Year Outcomes of Breast Cancer Surgical Management at the Colentina Surgical Clinic, Bucharest, Romania: A Descriptive Retrospective Analysis Between 2019 and 2023

by Cristian Botezatu, Daniel-Ovidiu Costea, Martina Nichilò, Angela Madalina Lazar, Dan Andraș, Mircea-Ion Radu and Bogdan Mastalier

Life 2025, 15(1), 92; https://doi.org/10.3390/life15010092 - 14 Jan 2025

Abstract

Background: Breast cancer still represents the most commonly diagnosed cancer among women, accounting for 12.5% of all new annual cancer cases worldwide. In Romania in 2020, breast cancer was the most common, with a share of new cases of 26.9%, far behind the [...] Read more.

Background: Breast cancer still represents the most commonly diagnosed cancer among women, accounting for 12.5% of all new annual cancer cases worldwide. In Romania in 2020, breast cancer was the most common, with a share of new cases of 26.9%, far behind the rates of colon cancer (11.8%) and cervix cancer (7.5%). The aim of this study is to reveal five years of experience in treating breast cancer at the Surgical Clinic of the Colentina Hospital in Bucharest, Romania. Methods: Retrospective analysis, including 68 patients admitted to our clinic between January 2019 and December 2023 undergoing modified radical mastectomy, sectorectomy, or subcutaneous mastectomy. Results: Madden-modified radical mastectomy with total excision of the axillary lymph nodes accounted for 77.94% of surgeries, with a complication rate of 13.2%, represented by lymphedema of the ipsilateral arm and prepectoral seroma. Conclusions: Continuous innovation regarding early diagnosis and treatment methods in our surgical clinic will, hopefully, contribute to improving the outcomes of our patients. Full article

(This article belongs to the Special Issue Current Approaches and Future Directions in the Treatment of Gynecologic Tumors: Molecular Biology, Diagnostic Advancements and Targeted Therapeutic Strategies)

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12 pages, 215 KiB

Open AccessArticle

Physical Activity Types Among School-Aged Children During COVID-19 Pandemic in Saudi Arabia: A Cross-Sectional Study

by Mshari Alghadier, Reem M. Basuodan, Afnan Gmmash and Reem A. Albesher

Life 2025, 15(1), 91; https://doi.org/10.3390/life15010091 - 14 Jan 2025

Abstract

Background: The COVID-19 pandemic may have had long-lasting detrimental effects on children’s physical health. Previous studies have shown that children’s participation in physical activity (PA) declined during the pandemic. This study examined the effect of the COVID-19 pandemic on PA type selection and [...] Read more.

Background: The COVID-19 pandemic may have had long-lasting detrimental effects on children’s physical health. Previous studies have shown that children’s participation in physical activity (PA) declined during the pandemic. This study examined the effect of the COVID-19 pandemic on PA type selection and the influence of gender, number of siblings, residence type, and caregiver education level on PA. Methods: Parents of Saudi children (ages 6–9 years) were recruited through convenience sampling and completed an online survey between July and August 2020. The parent-reported survey included demographics and PA types across three time periods (pre-, during, and post-lockdown). Chi-squared tests and logistic regression with pairwise comparisons were used to analyze the differences. Results: Parents reported that children (n = 361, mean age 7.7 ± 1.1 years) selected different PA types pre-COVID-19 pandemic more often than during the COVID-19 lockdown, such as swimming (16.9% vs. 12.8%), high-intensity jumping (9.8% vs. 6.6%), cycling (12.8% vs. 9.6%), football (14.3% vs. 6.1%), running (9.3% vs. 5.5%), virtual gaming exercise (5% vs. 3.2%), and playground activity (11.3% vs. 5.8%) (p < 0.05). Additionally, PA type was shown to be influenced by gender and residence type, with girls being 55% more likely to be physically active during COVID-19 compared to boys, and participants living in houses without private yards being less physically active compared to those who lived in houses with private yards. Conclusions: Children’s gender (boy vs. girl) and residence type (with private yards vs. without private yards) affected their PA level during the COVID-19 lockdown. These findings suggest that more effort should be directed toward understanding the influence of gender and house types in the selection of PA types. Full article

(This article belongs to the Special Issue Human Health Before, During, and After COVID-19)

13 pages, 5974 KiB

Open AccessArticle

Comparative Cranial Joint Variation in Three Different Lizards: Impact of Feeding Habit

by Elif Yıldırım Caynak, Kamil Candan, Yusuf Kumlutaş, Ahmet Gökay Korkmaz, Sezen Birlik, Dudu Mertgenç Yoldaş, Serkan Gül and Çetin Ilgaz

Life 2025, 15(1), 90; https://doi.org/10.3390/life15010090 - 13 Jan 2025

Abstract

The skull structure in vertebrates is closely related to feeding mode. This study examines the relationship between the cranial joint morphology variation among different lizard species [Eumesces schneideri (Daudin, 1802), Anguis colchica (Nordmann, 1840), and Eremias suphani (Başoğlu & Hellmich 1968)] and [...] Read more.

The skull structure in vertebrates is closely related to feeding mode. This study examines the relationship between the cranial joint morphology variation among different lizard species [Eumesces schneideri (Daudin, 1802), Anguis colchica (Nordmann, 1840), and Eremias suphani (Başoğlu & Hellmich 1968)] and their feeding habit. This study investigates the cranial anatomical correlates of distinct cranial kinesis models. Different cranial joints permitting intracranial mobility have been observed among these species using histological section and whole-mount techniques. The cranial joints are similar among species that generally exhibit cranial kinesis. The stomach contents of the species were analyzed, and E. schneideri has the highest prey diversity among the examined species, followed by E. suphani and A. colchica in that order. The study indicated that the prey preferences differ among three lizard species. While no plant material was detected in the stomach contents of E. suphani and E. schneideri, it was detected in A. colchica. The diet of the three lizards consisted of various species of small arthropods such as Arachnida, Lepidoptera, Coleoptera, Formicidae, and Gastropoda. Additionally, no significant differences were detected in SVL, head, and jaw size measurements between adult males and females of each species. Full article

(This article belongs to the Special Issue Feature Papers in Animal Science: 3rd Edition)

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11 pages, 1556 KiB

Open AccessArticle

In Vitro and In Vivo Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate Lactobacillus reuteri FW2

by Waqar Ahmad, Anam Nasir, Satya Prakash, Azam Hayat, Mujaddad ur Rehman, Shazia Khaliq, Kalsoom Akhtar, Munir Ahmad Anwar and Nayla Munawar

Life 2025, 15(1), 89; https://doi.org/10.3390/life15010089 - 13 Jan 2025

Abstract

Microorganisms synthesize diverse types of exopolysaccharides (EPSs). EPSs with varying structural and physical properties can demonstrate unique health benefits, which allow for their tailored applications as functional foods such as prebiotics. Levan, a fructose-based EPS, is gaining considerable attention as an effective prebiotic [...] Read more.

Microorganisms synthesize diverse types of exopolysaccharides (EPSs). EPSs with varying structural and physical properties can demonstrate unique health benefits, which allow for their tailored applications as functional foods such as prebiotics. Levan, a fructose-based EPS, is gaining considerable attention as an effective prebiotic to support the growth of beneficial gut bacteria. Consequently, this enhances digestive health, boosts the immune system, and reduces the risk of chronic diseases. Unfortunately, limited studies are available on levan-type EPSs to demonstrate their role as prebiotics. Therefore, in this study, we conducted in vitro and in vivo experiments, concerning intestinal cell integrity and metabolic syndrome, to assess the therapeutic potential of levan derived from Lactobacillus reuteri FW2. The in vitro experimental results revealed that levan improved the survival of impaired HT-29 epithelial cells of the intestine and also exerted antioxidant effects. In the in vivo experiments, mice fed with levan-supplemented feed exhibited low body weight gain, blood glucose, and serum cholesterol levels compared to the control group. These findings highlight the biotherapeutic potential of L. reuteri FW2-derived levan for improving metabolic syndrome and its associated aspects. It also signifies the need for a further detailed investigation based on clinical trials to include levan in dietary supplements for improved health and well-being. Full article

(This article belongs to the Special Issue Trends in Microbiology 2025)

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15 pages, 4018 KiB

Open AccessArticle

Endarachne binghamiae Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mice

by Sang-Hoon Lee, Sang-Seop Lee, Ga-Young Lee, Seung-Yun Han, Dong-Sub Kim, Bong-Ho Lee and Yung-Choon Yoo

Life 2025, 15(1), 88; https://doi.org/10.3390/life15010088 - 13 Jan 2025

Abstract

In this study, the anti-inflammatory effect of the hot water extract of Endarachne binghamiae (EB-WE), a type of marine brown algae, was investigated in LPS-stimulated RAW 264.7 cells and an acute lung injury (ALI) mouse model induced by intranasal LPS administration. Treatment with [...] Read more.

In this study, the anti-inflammatory effect of the hot water extract of Endarachne binghamiae (EB-WE), a type of marine brown algae, was investigated in LPS-stimulated RAW 264.7 cells and an acute lung injury (ALI) mouse model induced by intranasal LPS administration. Treatment with EB-WE significantly inhibited NO and pro-inflammatory cytokine (TNF-a and IL-6) production in LPS-stimulated RAW 264.7 cells. In mRNA analysis, the expression of pro-inflammatory cytokines, COX-2, and iNOS mRNAs, was down-regulated by EB-WE treatment. The phosphorylation of MAPK, IkB, and PI3K/AKT molecules responsible for signal pathways during inflammation in LPS-stimulated macrophages was also significantly inhibited by EB-WE. In an in vivo model for ALI, oral administration of EB-WE significantly reduced the level of pro-inflammatory cytokines (TNF-a, IL-1b, and IL-6) and chemokines (MCP-1, CXC-16, CXCL1, and TARC) in serum or bronchoalveolar lavage fluid (BALF) of mice. Similarly to the results in LPS-stimulated RAW 264.7 cells, treatment with EB-WE significantly inhibited intracellular signal pathways mediated by MAPK, IkB, and PI3K/AKT in lung tissues of mice with ALI, and also decreased the expression of mRNAs of inflammatory mediators such as TNF-a, IL-6, iNOS, and COX-2. Furthermore, the inhibitory effect of EB-WE on ALI was apparently confirmed in histological examination through lung tissue staining. Taken together, it is clear that EB-WE has potential activity to effectively ameliorate the inflammatory responses in macrophages through down-regulation of MAPK, NF-kB, and PI3K/AKT activation, and suppress acute lung injury induced by LPS. These findings strongly suggest that EB-WE is a promising natural product beneficial for developing preventive treatments and cures of inflammation-related diseases. Full article

(This article belongs to the Special Issue Inflammation and Natural Products: 2nd Edition)

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19 pages, 612 KiB

Open AccessArticle

The Influence of Lifestyle Modifications on Cardiovascular Outcomes in Older Adults: Findings from a Cross-Sectional Study

by Mohammed Almutairi, Ashwaq A. Almutairi and Abdulaziz M. Alodhialah

Life 2025, 15(1), 87; https://doi.org/10.3390/life15010087 - 13 Jan 2025

Abstract

Background: Cardiovascular disease (CVD) is a leading cause of morbidity and mortality among older adults. Lifestyle modifications, including diet, physical activity, and smoking cessation, are key to reducing cardiovascular risk. This study examines the combined effects of these behaviors on cardiovascular outcomes and [...] Read more.

Background: Cardiovascular disease (CVD) is a leading cause of morbidity and mortality among older adults. Lifestyle modifications, including diet, physical activity, and smoking cessation, are key to reducing cardiovascular risk. This study examines the combined effects of these behaviors on cardiovascular outcomes and their mediating mechanisms. Methods: A cross-sectional study was conducted among older adults (aged ≥ 60 years) in Riyadh, Saudi Arabia. Data on dietary quality, physical activity, and smoking status were collected using validated questionnaires. Cardiovascular outcomes, including low-density lipoprotein cholesterol (LDL), systolic blood pressure (SBP), and body mass index (BMI), were measured. A composite cardiovascular risk score was computed. Path analysis was employed to assess direct and indirect effects of lifestyle factors on cardiovascular outcomes. Results: Participants adhering to a healthy diet, engaging in regular physical activity, and avoiding smoking had significantly lower composite cardiovascular risk scores. Non-smoking status showed the strongest direct effect (β = −0.20, p = 0.006), while dietary quality and physical activity exhibited significant indirect effects mediated by LDL, SBP, and BMI. Combined adherence to multiple healthy behaviors resulted in the greatest reductions in cardiovascular risk. The path analysis highlighted dietary quality and physical activity as critical mediators of cardiovascular health improvements. Conclusions: Lifestyle modifications significantly reduce cardiovascular risk in older adults, with cumulative benefits observed for combined adherence to healthy behaviors. These findings emphasize the importance of comprehensive lifestyle interventions targeting diet, physical activity, and smoking cessation to promote cardiovascular health in aging populations. Full article

(This article belongs to the Special Issue Epidemiology Studies of Cardiovascular Diseases in Older Adults)

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22 pages, 11668 KiB

Open AccessArticle

Human Defence Factors in Different Gestational Week Placenta: A Pilot Study

by Andris Kamergrauzis, Mara Pilmane and Anna Junga

Life 2025, 15(1), 86; https://doi.org/10.3390/life15010086 - 13 Jan 2025

Abstract

Background: Numerous studies have shown the presence of multiple defence factors in placental tissue, although their role is partially understood; therefore, the aim of this study was to evaluate the expression of nuclear factor-kappa B (NF-κB); human beta-defensin 2, 3, and 4 (HBD-2,3,4); [...] Read more.

Background: Numerous studies have shown the presence of multiple defence factors in placental tissue, although their role is partially understood; therefore, the aim of this study was to evaluate the expression of nuclear factor-kappa B (NF-κB); human beta-defensin 2, 3, and 4 (HBD-2,3,4); cathelicidine (LL-37); heat shock protein 60 (HSP60); and interleukin 10 (IL-10) in dissimilar gestational week placental tissue and display correlations between immunoreactive cells. Methods: A total of 15 human placental tissue samples were acquired from mothers with different gestational weeks: 28, 31, and 40. Routine staining and immunohistochemistry for the samples were executed. The evaluation of data was performed with semi-quantitative methods, and, for statistical analysis, the Kruskal–Wallis test was used. Spearman’s rank correlation was used for calculating correlations. Results: NF-κB, HBD- 2,3,4, HSP60, and IL-10 expression were discovered in every examined placental tissue cell type. LL-37 expression was found only in Hofbauer cells. A rise in expression with higher gestational weeks was noted in LL-37-positive Hofbauer cells (p = 0.03), HBD-3-positive cytotrophoblasts (p = 0.007), endothelial cells (p = 0.024), extraembryonic mesodermal cells (p = 0.004), and HBD-4-positive endothelial cells (p = 0.001). Numerous statistically significant moderate and strong positive correlations between defence factors were discovered. Conclusions: The persistence of Hofbauer cell accumulations underlines the growing significance of placental macrophages in placental protection. The expression of positive defence factors and a rise in expression in tissue protection factors (HBD-3, LL-37, HBD-4) in higher gestational weeks may indicate these factors as the most significant protectors of the placenta in ontogenetic aspects. The high number of statistically significant positive and negative correlations between positive cells show a strong network to sustain distressed placental growth and therefore pregnancy. Full article

(This article belongs to the Section Reproductive and Developmental Biology)

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