Processes

Cassava flour (CF) is a suitable representative and one of the easiest shelf-stable food products of the edible portion of the highly perishable cassava root (Manihot esculenta Crantz). The quality and type of CF are dependent on processing variables. Broadly categorized into fermented and unfermented CF, unfermented CF is white, odorless, and bland, while fermented CF has a sour flavor accompanied by its characteristic odor. The use of fermented CF as a composite is limited because of their off-odors. Modifications in CF processing have given rise to prefixes such as: modified, unmodified, gelatinized, fortified, native, roasted, malted, wet, and dry. Consumed alone, mostly in reconstituted dough form with soups, CF may also serve as a composite in the processing of various flour-based food products. Fermenting with microorganisms such as Rhizopus oryzae and Saccharomyces cerevisiae results in a significant increase in the protein content and a decrease in the cyanide content of CF. However, there are concerns regarding its safety for consumption. Pre-gelatinized CF has potential for the textural and structural improvement of bakery products. The average particle size of the CF also influences its functional properties and, subsequently, the quality of its products. Cassava flour is best stored at ambient temperature. Standardizing the processing of CF is a challenge because it is mostly processed in artisanal units. Furthermore, each variety of the root best suits a particular application. Therefore, understanding the influence of processing variables on the characteristics of CF may improve the utilization of CF locally and globally. Full article

Covering steel surfaces with suitable materials with the capacity to protect against corrosion represents a challenge for both research and industry, as steel, due to its paramount utility, is the most recycled material. This study presents the realization of new sandwich type materials based on 5,10-(4-carboxy-phenyl)-15,20-(4-phenoxy-phenyl)-porphyrin or 5,15-(4-carboxy-phenyl)-10,20-diphenylporphyrin and MnTa₂O₆ designed to improve corrosion inhibition of steel in aggressive media. The thin films, designed as single- or sandwich-type structures were obtained on carbon steel through the drop-casting technique. Morphological investigations of thin films were carried out by field emission-scanning electron microscopy (SEM) and atomic force microscopy (AFM). The inhibition of a steel corrosion process was evaluated in an aggressive environment of 0.1 M HCl by performing electrochemical investigations such as open circuit potential (OCP) and the potentiodynamic polarization technique. The influence of variations in the cathodic Tafel slopes β_c and anodic Tafel slopes β_a over the corrosion rates was discussed. The best corrosion inhibition efficiency of 91.76% was realized by the steel electrode covered with sandwich-type layers of 5,15-(4-carboxy-phenyl)-10,20-diphenylporphyrin on the bottom layer and MnTa₂O₆ on the top. The effect of location of the COOH groups in the cis or trans position on the tetrapyrrolic ring was also discussed to understand the corrosion inhibition mechanism. Full article

Varied cereal plants including, mushrooms, yeast, bacteria and algae are important sources of β-glucans, and many extraction procedures have been used in order to recover these valuable naturally occurring polysaccharides. The rheological and molecular properties of β-glucans can be utilized to be incorporated into various foods and to offer properties extremely beneficial to human health. Their functional effects are mainly determined by their molecular and structural characteristics. Consumption of foods fortified and enriched with β-glucans can contribute to the treatment of certain chronic diseases. Reduced cholesterol, cardiovascular and diabetic risk and moderate glycemic response of foods have been recorded with the consumption of these biologically active compounds. In addition, β-glucans are characterized by anti-cancer, antioxidant, anti-inflammatory and antiviral activities. As β-glucans interact with the foods in which they are incorporated, this review aims to discuss recent applications with quality and nutritional results of β-glucans incorporation with foods such as beverages, dairy, bakery, meat and pasta products, as well as their addition in animal feeds and their uses in other fields such as medicine. Full article

The black soldier fly larvae (BSFL) is a sustainable ingredient for feed applications, biofuels, composite materials and other biobased products. Processing BSFL to obtain lipid and protein fractions with enhanced functional properties as a suitable replacement for conventional feed ingredients has gained considerable momentum. In this regard, a novel and sustainable wet mode fractionation (WMF) scheme for BSFL was explored. Fresh BSFL were steam blanched and pulped to obtain BSFL juice and juice press cake. Subsequent treatment of BSFL juice employing homogenization or enzyme incubation and further centrifugation resulted in the obtention of four different BSFL fractions (Lipid—LF; Cream—CF; Aqueous—AF; and Solid—SF). Total energy consumption for a batch BSFL (500 g) WMF process was 0.321 kWh. Aqueous and solid fractions were the predominant constituents of BSFL juice. Lauric acid (44.52–49.49%) and linoleic acid (19.12–20.12%) were the primary fatty acids present in BSFL lipids. Lipid hydrolysis was observed in lipids belonging to the solid (free fatty acids > triacylglycerides) and cream fractions. Aqueous fraction proteins (ctrl) displayed superior emulsion stability and foam capacity than other treatments. Juice press cake retained 60% of the total chitin content and the rest, 40%, was found in the solid fraction (ctrl). The material distribution of principal constituents in different fractions of the WMF process and amino acid profile was elucidated. Overall, the versatile WMF process proposed in this study involves simple unit operations to obtain functional ingredients from BSFL, which can be further explored by researchers and industry stakeholders. Full article

A selection flowchart that assists, through Computational Fluid Dynamics (CFD) simulations, the design of microfluidic experiments used to distinguish the performance in Chemical Enhanced Oil Recovery (CEOR) of two surfactants with very similar values of interfacial tension (IFT) was proposed and its use demonstrated. The selection flowchart first proposes an experimental design for certain modified variables (: porosity, grain shape, the presence of preferential flowing channels, and injection velocity). Experiments are then performed through CFD simulations to obtain a set of response variables (: recovery factor, breakthrough time, the fractal dimension of flow pattern, pressure drop, and entrapment effect). A sensitivity analysis of regarding the differences in the interfacial tension (IFT) can indicate the CFD experiments that could have more success when distinguishing between two surfactants with similar IFTs (0.037 mN/m and 0.045 mN/m). In the range of modifiable variables evaluated in this study (porosity values of 0.5 and 0.7, circular and irregular grain shape, with and without preferential flowing channel, injection velocities of 10 ft/day and 30 ft/day), the entrapment effect is the response variable that is most affected by changes in IFT. The response of the recovery factor and the breakthrough time was also significant, while the fractal dimension of the flow and the pressure drop had the lowest sensitivity to different IFTs. The experimental conditions that rendered the highest sensitivity to changes in IFT were a low porosity (0.5) and a high injection flow (30 ft/day). The response to the presence of preferential channels and the pore shape was negligible. The approach developed in this research facilitates, through CFD simulations, the study of CEOR processes with microfluidic devices. It reduces the number of experiments and increases the probability of their success. Full article

In this article, a numerical study is performed on a Trobme wall in a tropical city for two seasons, summer and winter. A $1\times 1.5 \mathrm{m}$ Trobme wall with a thickness of 15 cm is designed and analyzed. A 1-inch-diameter tube filled with PCM is used to enhance efficiency. The wall is analyzed at different times of the day for the two cold and hot seasons for different sizes of wall holes in the range of 70 to 17.5 cm when the wall height is 20 cm. A fluid simulation software is employed for the simulations. The problem variables include different hours of the day in the two cold and hot seasons, the presence or absence of PCM, as well as the size of the wall hole. The results of this simulation demonstrate that the maximum outlet temperature of the Trobme wall occurs at 2 P.M. Using PCM on the wall can allow the wall to operate for longer hours in the afternoon. However, the use of PCM reduces the outlet wall temperature in the morning. The smaller the size of the wall hole, the more air can be expelled from the wall. Full article

Aiming at the problem of uneven clustering and the unreasonable energy consumption of LEACH protocol in the perception layer of IoT-based microgrids of static nodes; in this paper, we propose a stationary-node energy-based routing protocol (SERP). First, we select a dynamic cluster radius for clustering to meet the actual needs of the network during clustering. Then, to solve the problem that the number of cluster heads is difficult to determine, a dynamic optimal cluster head ratio is adopted. The dynamic optimal cluster head ratio can be obtained by minimizing the total energy consumption of cluster formation and the stable transmission phase, which can improve the efficiency of network transmission. Finally, by setting the residual energy factor and distance factor to improve the calculation of the cluster head election threshold, the energy load of the network is more uniform, and the location of the cluster head is more reasonable. Compared with the LEACH protocol and the HEED protocol, the simulation results show that the SERP protocol can effectively prolong the lifetime of the whole network. Full article

An open system based on physical adsorption phenomena with humid air and zeolite 13X is herein discussed for residential heat storage purposes. A model has been developed to describe the conservation of mass and heat in the system. A simplified approach of a complete model describing both mass conservation in the macroporous and microporous domains is used based on the linear driving force (LDF) model. Local mass and heat transfer properties have been used. To describe the equilibrium, the Aranovich–Donohue isotherm model is selected. As an example, the developed model is compared and fitted to experimental data from a pilot scale system. A parametric study on operating and design parameters is given to understand their effect on the amount and/or duration of heat supply, concentration, and temperature profiles. The studied parameters are the inlet adsorbate concentration, fluid temperature, and velocity, as well as particle and zeolite crystal sizes. This analysis shows that an identification of values for the set of parameters tested can possibly suit the energy needs for a case study of domestic heat supply. Future work will focus on the optimization of these parameters. Full article

In this study, a method for effectively estimating the airflow rate of the turbine of an oscillating water column (OWC) pilot plant was developed. The validity of the proposed method was verified through computational fluid dynamics simulations. The method was applied to estimate the airflow rate in irregular wave states based on the operation data obtained for the Yongsoo OWC pilot plant installed in the western seas of Jeju Island, South Korea. As an alternative to estimating the airflow rate of the OWC pilot plant, the impulse turbine performance chart-based interpolation method is introduced, and it is shown that the airflow rate time series calculated using the two methods were in good agreement. Full article

Soft magnetic materials are important functional materials in the electrical engineering, radio, and high-tech fields, but thin and brittle flakes present challenges to the manufacturing industry. In this study, the effect and mechanism of saccharin sodium in reducing the internal stress of Fe-Ni magnetic films were analyzed. The effects of the pH value, temperature, and the concentration of saccharin sodium on the deposition process of Fe-Ni alloys were investigated. The polarization curve of the Fe-Ni alloy deposition process was measured by using a multifunctional electrochemical workstation, and the morphology and crystal structure were measured by a scanning electron microscope (SEM) and X-ray diffraction (XRD). The results show that saccharin sodium significantly reduced the stress of the iron-nickel magnetic film; the mechanism through which the internal stress was reduced is analyzed in this paper. Briefly, the Fe²⁺ and the amino group of saccharin sodium synthesized a metal complex with positive charge on the surface of the electrode, which prevented the hydrogen ions from approaching the cathode and increased the discharge activation energy of the hydrogen ion, which reduced the hydrogen evolution and improved the internal stress of the coating. This research will help to solve the challenges of producing magnetic film, and promotes the application of new stress-reducing agents. Full article

In this study we developed a brushless DC (BLDC) slotless motor with toroidal winding. The proposed toroidal winding is a method of winding a coil around a ring-type stator yoke in the circumferential direction. As there is no need for a slot or tooth structure, it can be designed with a slotless motor structure that is advantageous for vibration and noise. The basic principle of operation and motor characteristics of a slotless motor with toroidal winding were explained using an analytical method and finite element analysis (FEA). Further, the air gap flux density, winding factor, and back electromotive force (EMF) for changes in the winding angle and number of coil turns were calculated using the analytical method and compared with the FEA results. Finally, the resistance, back EMF, cogging torque, and performance of the prototype were measured and compared with the FEA results. The results show that the air gap flux density and winding factor were approximately the same with an error of <2%, while the back EMF had an error of ~10% from the analysis result. Thus, the proposed slotless motor provides a basic design for conveniently manufacturing brushless DC (BLDC) slotless motors with toroidal windings. Full article

In some fermentation systems, whey components (lactose, proteins and minerals) can produce isopentyl acetate (IA). An analysis of the best conditions for IA production with Kluyveromyces marxianus was developed in this work. The experiment design was two-factor and three-level design based on a response surface methodology (RSM) using Design-Expert^® software. The analysis of anomeric protons by nuclear magnetic resonance (1H-NMR) showed 81.25% of β lactose content. This characteristic favored the production of IA. The maximum output (Mp) of IA, determined by gas chromatography, was 9.52 g/L (p < 0.05). The central composite design (CCD) was used to perform the factor analysis. Results showed that concentrations of 0.03 (g/L) ammonium sulphate and 0.3 (v/v) of isoamyl alcohol are the best conditions for a maximum rate of IA production. The production of IA can reduce the discharge of whey, allowing its reuse and revaluation. Full article

Several optimization models, which consider economic and environmental perspectives, have been developed recently to support the sustainable biomass-to-biofuel supply chain (BBSC) design. All of the economic-environmental optimization models rely on solving long-term planning problems with a conventional hierarchical approach, where tactical decisions are made based on the optimal strategic decisions from the strategic-level model, despite it arousing non-optimal solutions. Moreover, almost all of them have used non-monetary-based environmental indicators, which result in difficulties with clarity when comparing with economic objectives. Therefore, in this work, an effort is made to develop a more reliable planning strategy that offers optimal strategic and tactical decisions simultaneously and maximizes the economic and environmental benefits. Furthermore, the environmental performance of the BBSC has been assessed in terms of monetary value by adopting an ecocost approach after performing an LCA on the system. The integrated model is applied in the real biofuel sector of Ethiopia to optimize the country’s bioethanol and biodiesel supply chain over a 20-year horizon. Despite the abrupt rise in the model size, with it being a real countrywide case with many variables and large quantities of data, an alternative semi-heuristic method that offers a feasible solution to the multi-objective problem is provided. Full article

A new catalase amperometric biosensor for hydroperoxides detection has been built as part of research aimed at the development of biosensors based on layered double hydroxides (LDH) used as support for enzyme immobilization. The fabricated device differs from those developed so far, usually based on an LDH enzyme nanocomposite adsorbed on a glassy carbon (GC) electrode and cross-linked by glutaraldehyde, since it is based on an amperometric gas diffusion electrode (Clark type) instead of a GC electrode. The new biosensor, which still uses LDH synthesized by us and catalase enzyme, is robust and compact, shows a lower LOD (limit of detection) value and a linearity range shifted at lower concentrations than direct amperometric GC biosensor, but above all, it is not affected by turbidity or emulsions, or by the presence of possible soluble species, which are reduced to the cathode at the same redox potential. This made it possible to carry out accurate and efficient determination of H₂O₂ even in complex or cloudy real matrices, also containing very low concentrations of hydrogen peroxide, such as milk and cosmetic products, i.e., matrices that would have been impossible to analyze otherwise, using conventional biosensors based on a GC–LDH enzyme. An inaccuracy ≤7.7% for cosmetic samples and ≤8.0% for milk samples and a precision between 0.7 and 1.5 (as RSD%), according to cosmetic or milk samples analyzed, were achieved. Full article

In this work, the relaxation parameter (τ) and fractionality order (α) in the fractional single phase lag (FSPL) non-Fourier heat conduction model are estimated by employing the conjugate gradient inverse method (CGIM). Two different physics of skin tissue are chosen as the studied cases; single and three-layer skin tissues. Single-layer skin is exposed to laser radiation having the constant heat flux of Q_in. However, a heat pulse with constant temperature is imposed on the three-layer skin. The required inputs for the inverse problem in the fractional diffusion equation are chosen from the outcomes of the dual phase lag (DPL) theory. The governing equations are solved numerically by utilizing implicit approaches. The results of this study showed the efficiency of the CGIM to estimate the unknown parameters in the FSPL model. In fact, obtained numerical results of the CGIM are in excellent compatibility with the FSPL model. Full article

Our body composition is enormously influenced by our lifestyle choices, which affect our health and longevity. Nutrition and physical activities both impact overall metabolic condition, thus, a positive energy balance causes oxidative stress and inflammation, hastening the development of metabolic syndrome. With this knowledge, boosting endogenous and exogenous antioxidants has emerged as a therapeutic strategy for combating metabolic disorders. One of the promising therapeutic inventions is the use of alkaline reduced water (ARW). Aside from its hydrating and non-caloric properties, ARW has demonstrated strong antioxidant and anti-inflammatory properties that can help stabilize physiologic turmoil caused by oxidative stress and inflammation. This review article is a synthesis of studies where we elaborate on the intra- and extracellular effects of drinking ARW, and relate these to the pathophysiology of common metabolic disorders, such as obesity, diabetes mellitus, non-alcoholic fatty liver disease, and some cancers. Highlighting the health-promoting benefits of ARW, we also emphasize the importance of maintaining a healthy lifestyle by incorporating exercise and practicing a balanced diet as forms of habit. Full article

This study evaluates the performance of different agricultural by-products to identify the potential effect of independent variables, using as the dependent variable the biogas production. A Box–Behnken experimental design was carried out in a pilot-scale plant of four stirred stainless-steel digesters under mesophilic semi-continuous digestion. The results obtained support the creation of a technical framework to scale up the process and further evaluation of the potential environmental impacts through life cycle assessment (LCA) methodology. A stable behaviour was achieved in 12 of the 13 experiments proposed. The highest value of daily biogas production was 2200.15 mL day⁻¹ with a stabilization time of 14 days, an organic loading rate of 4 g VS feed daily, low C/N ratio and a 1:1 relation of nitrogen providers. The concentrations of CH₄ remained stable after the production stabilization and an average biogas composition of 60.6% CH₄, 40.1% CO₂ and 0.3% O₂ was obtained for the conditions mentioned above. Therefore, the real scale plant was estimated to manage 2.67 tonnes of residual biomass per day, generating 369.69 kWh day⁻¹ of electricity. The LCA analysis confirms that the co-digestion process evaluated is a feasible and environmentally sustainable option for the diversification of the Colombian energy matrix and the development of the agro-industrial sector. Full article

SARS-COVID-19 vaccine supply for the total worldwide population has a bottleneck in manufacturing capacity. Assessment of existing messenger ribonucleic acid (mRNA) vaccine processing shows a need for digital twins enabled by process analytical technology approaches in order to improve process transfer for manufacturing capacity multiplication, a reduction in out-of-specification batch failures, qualified personal training for faster validation and efficient operation, optimal utilization of scarce buffers and chemicals and speed-up of product release by continuous manufacturing. In this work, three manufacturing concepts for mRNA-based vaccines are evaluated: Batch, full-continuous and semi-continuous. Technical transfer from batch single-use to semi-continuous stainless-steel, i.e., plasmid deoxyribonucleic acid (pDNA) in batch and mRNA in continuous operation mode, is recommended, in order to gain: faster plant commissioning and start-up times of about 8–12 months and a rise in dose number by a factor of about 30 per year, with almost identical efforts in capital expenditures (CAPEX) and personnel resources, which are the dominant bottlenecks at the moment, at about 25% lower operating expenses (OPEX). Consumables are also reduceable by a factor of 6 as outcome of this study. Further optimization potential is seen at consequent digital twin and PAT (Process Analytical Technology) concept integration as key-enabling technologies towards autonomous operation including real-time release-testing. Full article

The uncontrolled release of pharmaceutical drugs into the environment raised serious concerns in the last decades as they can potentially exert adverse effects on living organisms even at the low concentrations at which they are typically found. Among them, platinum based cytostatic drugs (Pt CDs) are among the most used drugs in cancer treatments which are administered via intravenous infusion and released partially intact or as transformation products. In this review, the studies on environmental occurrence, transformation, potential ecotoxicity, and possible treatment for the removal of platinum cytostatic compounds are revised. The analysis of the literature highlighted the generally low total platinum concentration values (from a few tens of ng L⁻¹ to a few hundred μg L⁻¹) found in hospital effluents. Additionally, several studies highlighted how hospitals are sources of a minor fraction of the total Pt CDs found in the environment due to the slow excretion rate which is longer than the usual treatment durations. Only some data about the impact of the exposure to low levels of Pt CDs on the health of flora and fauna are present in literature. In some cases, adverse effects have been shown to occur in living organisms, even at low concentrations. Further ecotoxicity data are needed to support or exclude their chronic effects on the ecosystem. Finally, fundamental understanding is required on the platinum drugs removal by MBR, AOPs, technologies, and adsorption. Full article