Animals

11 pages, 3872 KB

Open AccessCase Report

Fibrosarcoma Originating from the Pleura in Cotton-Top Tamarin [Oedipomidas (Saguinus) oedipus, Linnaeus 1758]

by János Gál, Endre Sós, Árisz Ziszisz, Márton Hoitsy, Míra Mándoki, Dóra Csatári and Zoltán Vincze

Animals 2026, 16(2), 195; https://doi.org/10.3390/ani16020195 (registering DOI) - 9 Jan 2026

Abstract

This report describes postmortem findings after the sudden death of a captive-bred middle-aged male cotton-top tamarin (Saguinus oedipus). A firm structure was attached to the pleura in the chest cavity and was assessed as a primary tumor without obvious metastasis. Histologically, [...] Read more.

This report describes postmortem findings after the sudden death of a captive-bred middle-aged male cotton-top tamarin (Saguinus oedipus). A firm structure was attached to the pleura in the chest cavity and was assessed as a primary tumor without obvious metastasis. Histologically, the mass showed heterogenicity with immunohistochemistry (IHC) reactivity to vimentin, suggesting a diagnosis of fibrosarcoma of mesenchymal origin. Full article

(This article belongs to the Special Issue Disease and Health in Free-Ranging and Captive Wildlife: Second Edition)

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19 pages, 23779 KB

Open AccessArticle

Unveiling the Genomic Landscape of Yan Goose (Anser cygnoides): Insights into Population History and Selection Signatures for Growth and Adaptation

by Shangzong Qi, Zhenkang Ai, Yuchun Cai, Yang Zhang, Wenming Zhao and Guohong Chen

Animals 2026, 16(2), 194; https://doi.org/10.3390/ani16020194 (registering DOI) - 8 Jan 2026

Abstract

The Yan goose (YE, Anser cygnoides) is a valuable indigenous poultry genetic resource, renowned for its superior meat quality and environmental adaptability. Despite its economic importance, the genetic basis underlying these adaptive traits remains unclear. In this study, we employed whole-genome resequencing [...] Read more.

The Yan goose (YE, Anser cygnoides) is a valuable indigenous poultry genetic resource, renowned for its superior meat quality and environmental adaptability. Despite its economic importance, the genetic basis underlying these adaptive traits remains unclear. In this study, we employed whole-genome resequencing (WGS) to perform high-throughput sequencing on a conserved population of 15 samples. Bioinformatic analyses were conducted to systematically evaluate the population’s genetic structure, and a genome-wide scan for selection signals related to economically significant traits was performed using the integrated haplotype score (iHS) method. An average of 4.43 million high-quality SNPs were identified, which were predominantly located in intergenic and intronic regions. Population structure analysis revealed a close genetic relationship within the conserved population of YE, with no significant lineage stratification observed. Pairwise sequentially Markovian coalescent (PSMC) analysis indicated that the YE underwent a severe genetic bottleneck during the Last Glacial Maximum (LGM), followed by gradual population recovery in the early Neolithic period. Genome-wide selection signal scanning identified multiple genomic regions under strong selection, annotating key genes associated with growth and development (e.g., GHRL, AKT1, and MAPK3), lipid deposition (e.g., PLPP4, SAMD8, and LPIN1), and disease resistance and stress resilience (e.g., TP53, STAT3). Functional enrichment analysis revealed significant enrichment of these genes in pathways related to glycerophospholipid metabolism (p < 0.01), purine metabolism (p < 0.01), and immune response (p < 0.01). This study not only provides a theoretical foundation for the scientific conservation of the YE germplasm resources but also offers valuable genomic resources for identifying functional genes underlying important economic traits and advancing molecular breeding strategies. Full article

(This article belongs to the Special Issue Genetic Diversity and Conservation of Local Poultry Breeds)

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23 pages, 647 KB

Open AccessArticle

Mild Ozone-Induced Oxidative Stress Modulates the Activity and Viability of Porcine Neutrophils and Monocytes

by Dominika Nguyen Ngoc, Jose Luis Valverde Piedra, Andrzej Milczak, Tomasz Szponder, Beata Drzewiecka, Aleksandra Pyzerska, Małgorzata Kowalczyk, Mateusz Fila, Ewa Tomaszewska, Arti Ahluwalia and Joanna Wessely-Szponder

Animals 2026, 16(2), 193; https://doi.org/10.3390/ani16020193 (registering DOI) - 8 Jan 2026

Abstract

Ozone (O₃) is a reactive oxidant increasingly applied in biomedical settings, yet its dose-dependent effects on innate immune cells, particularly those from non-human species, remain insufficiently defined. Within a One Health framework, this study examined how two clinically relevant O₃ [...] Read more.

Ozone (O₃) is a reactive oxidant increasingly applied in biomedical settings, yet its dose-dependent effects on innate immune cells, particularly those from non-human species, remain insufficiently defined. Within a One Health framework, this study examined how two clinically relevant O₃ exposure regimens (30 µg/mL and 90 µg/mL) affect porcine neutrophils and monocytes isolated from peripheral blood. Cell viability, reactive oxygen and nitrogen species (RONS) production, and the activity of key enzymes (myeloperoxidase, elastase, alkaline phosphatase, arginase) were assessed at 1 h and 24 h post-exposure. The lower dose induced mild functional activation without compromising viability, whereas the higher dose triggered pronounced oxidative stress, enhanced degranulation, and reduced neutrophil viability by more than 60%. Neutrophils exhibited a stronger and more dynamic response than monocytes, which retained viability and differentiation capacity at 30 µg/mL but showed impaired function at 90 µg/mL. These findings highlight the dual nature of O₃, where controlled exposure may support immunomodulation, while excessive dosing disrupts cell function. Defining safe and effective therapeutic windows remains critical for future applications. Full article

(This article belongs to the Section Pigs)

27 pages, 6289 KB

Open AccessArticle

Integrated Analysis of Histophysiological Responses and Transcriptome–Metabolome Mechanisms in Coelomactra antiquata Under Ammonia Nitrogen Stress

by Dongming Huang, Sican Cai, Yongkang Hou, Hongli Qin, Yinyin Deng and Zhimin Li

Animals 2026, 16(2), 192; https://doi.org/10.3390/ani16020192 (registering DOI) - 8 Jan 2026

Abstract

Coelomactra antiquata, a marine bivalve of high nutritional and economic value, lacks comprehensive data on its toxic responses and adaptive mechanisms to ammonia nitrogen. This study integrated histophysiology, transcriptomics, and metabolomics to investigate its ammonia tolerance and molecular mechanisms, determining a 48 [...] Read more.

Coelomactra antiquata, a marine bivalve of high nutritional and economic value, lacks comprehensive data on its toxic responses and adaptive mechanisms to ammonia nitrogen. This study integrated histophysiology, transcriptomics, and metabolomics to investigate its ammonia tolerance and molecular mechanisms, determining a 48 h LC₅₀ of 99.06 mg/L and a sublethal concentration of 9.91 mg/L. After 48 h of sublethal ammonia stress, SOD, CAT, GLDH, and GS activities in gill and hepatopancreas significantly increased, with notable changes in MDA, Gln, and urea contents, confirming disruption of antioxidant defense and nitrogen metabolism homeostasis. Tissue sections revealed irreversible histopathological damage to key tissues. Omics analyses identified 7823 differentially expressed genes (DEGs) and 737 differentially expressed metabolites (DEMs) in hepatopancreas. DEGs were enriched in metabolic pathways and multiple immune-related signaling pathways (e.g., NF-kappa B, RIG-I-like receptor), while DEMs were primarily involved in processes such as protein digestion/absorption, aminoacyl-tRNA biosynthesis, and amino acid metabolism. Research data indicate that ammonia nitrogen stress primarily regulates the antioxidant function and nitrogen metabolism homeostasis of C. antiquata by activating multiple immune- and metabolism-related pathways. This first systematic multi-omics study elucidates C. antiquata’s tolerance to ammonia nitrogen and its molecular responses, filling a gap in environmental toxicology research for sustainable aquaculture and genomic studies. Full article

(This article belongs to the Special Issue Recent Research on Shellfish Aquaculture and Reproduction)

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11 pages, 1337 KB

Open AccessArticle

Identification, Serotyping and Antimicrobial Resistance in Ornithobacterium rhinotracheale Isolates from Turkeys in Poland Between 2016 and 2022

by Marek Blanda, Marcin Śmiałek, Joanna Kowalczyk and Olimpia Kursa

Animals 2026, 16(2), 191; https://doi.org/10.3390/ani16020191 - 8 Jan 2026

Abstract

Respiratory diseases in poultry caused by Ornithobacterium rhinotracheale represent a significant challenge from both therapeutic and economic perspectives. Treatment of them, which uses antimicrobial agents, improperly contributes to the emergence and dissemination of multidrug-resistant strains within avian populations. The present study, based on [...] Read more.

Respiratory diseases in poultry caused by Ornithobacterium rhinotracheale represent a significant challenge from both therapeutic and economic perspectives. Treatment of them, which uses antimicrobial agents, improperly contributes to the emergence and dissemination of multidrug-resistant strains within avian populations. The present study, based on O. rhinotracheale isolates (n = 733) obtained from the respiratory tract, joints, and organs of turkeys in Poland between 2016 and 2022, was conducted to assess the antimicrobial resistance of the strains and identify their serotypes. All O. rhinotracheale isolates were tested for antimicrobial susceptibility using the disk-diffusion method. The results of this study indicated that amoxicillin/clavulanic acid, doxycycline, florfenicol, and lincomycin/spectinomycin were the most effective agents against the isolates from turkeys. The findings showed that serotype I was the most prevalent among the tested turkey strains in Poland and was the serotype to which 29% of the O. rhinotracheale isolates affiliated. Full article

(This article belongs to the Section Poultry)

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22 pages, 5316 KB

Open AccessArticle

Density and Coexistence Patterns of an Apex Carnivore (Panthera pardus) and a Mesocarnivore (Caracal aurata) in Northern Congo Forests

by Sarah Tossens, Zoe Woodgate, Jean-Louis Doucet, Philipp Henschel, Adrien André, Johan Michaux and Marine Drouilly

Animals 2026, 16(2), 190; https://doi.org/10.3390/ani16020190 - 8 Jan 2026

Abstract

Understanding how carnivores coexist is central to ecological theory and conservation. Coexistence among sympatric species arises through niche partitioning across spatial, temporal, and trophic dimensions, yet these mechanisms remain poorly explored in Central African forests where leopards (Panthera pardus) and African [...] Read more.

Understanding how carnivores coexist is central to ecological theory and conservation. Coexistence among sympatric species arises through niche partitioning across spatial, temporal, and trophic dimensions, yet these mechanisms remain poorly explored in Central African forests where leopards (Panthera pardus) and African golden cats (Caracal aurata) act as dominant and subordinate carnivores. Using camera trap data and molecular scat analyses from two sites in northern Congo, we provided the first robust leopard density estimates for the region (i.e., semideciduous forests in Central Africa) and assessed coexistence mechanisms between the two felids across spatial, temporal, and trophic axes. Spatially explicit capture–recapture models revealed comparable leopard densities across sites (5–6 individuals/100 km²), exceeding the regional average for Central and East Africa. Spatiotemporal occupancy models indicated spatial and temporal overlap, with no evidence of predictive or reactive temporal avoidance, though fine-scale co-occurrence declined near linear forest features (i.e., main rivers and roads) where both species’ marginal occupancy was highest. Conversely, dietary analyses showed trophic segregation: leopards consumed medium- to large-sized ungulates (>20 kg), whereas golden cats relied on smaller prey (≤5 kg), identifying trophic partitioning as the main axis facilitating coexistence in this prey-rich system. Maintaining prey diversity and minimizing disturbance are key to sustaining both species and their coexistence mechanisms. Such multidimensional approaches are essential to understand intraguild interactions and anticipate community shifts under increasing pressure. Full article

(This article belongs to the Section Ecology and Conservation)

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19 pages, 321 KB

Open AccessArticle

Dietary-Amylose/Amylopectin-Ratio-Modulated Growth Performance, Intestinal Health, and Lipid Metabolism in Goslings Fed Low-Protein Diets

by Xiyuan Xing, Xucheng Zheng, Xuan Li, Zhi Yang, Haiming Yang and Zhiyue Wang

Animals 2026, 16(2), 189; https://doi.org/10.3390/ani16020189 - 8 Jan 2026

Abstract

This study investigated the effects of amylose/amylopectin (AM/AP) ratios in low-protein (LP) diets on the growth performance, fat deposition, and nutrient utilization in goslings. A total of 288 healthy, 35-day-old male Jiangnan White Geese were randomly divided into four treatment groups: one group [...] Read more.

This study investigated the effects of amylose/amylopectin (AM/AP) ratios in low-protein (LP) diets on the growth performance, fat deposition, and nutrient utilization in goslings. A total of 288 healthy, 35-day-old male Jiangnan White Geese were randomly divided into four treatment groups: one group fed a normal protein diet (16%) with an AM/AP ratio of 0.34 (NPR_0.34), and three groups fed low protein diets (14%) with different AM/AP ratios (LPR_0.26, LPR_0.34, LPR_0.44). Each group consisted of six replicates, with 12 geese per replicate, and they were fed for 28 days. The results showed that the body weight at 63 days and average daily gain (ADG) of the LPR_0.44 group geese were significantly higher than those of the other groups (p < 0.01), while the feed-to-gain ratio (F/G) was lower (p < 0.05). The abdominal and mesenteric fat contents were lower in the LPR_0.44 group than in the LPR_0.26 group (p < 0.05), whereas the breast and leg muscle yields were higher (p < 0.05). The breast muscle redness (a*) of the LPR_0.34 and LPR_0.44 groups was higher than in the NPR_0.34 group at 45 min (p < 0.05). The levels of C6:0, C8:0, C11:0, C12:0, and C13:0 in breast muscle saturated fatty acids (SFAs) of the LPR_0.44 group were higher, while that of C18:0 was lower compared with the LPR_0.26 group (p < 0.05). The serum total cholesterol (TC) and triglycerides (TGs) in the LPR_0.44 group were lower than in the LPR_0.26 group (p < 0.05). Hepatic lipase (HL) activity was significantly lower in the LPR_0.44 group (p < 0.01). Regarding hepatic fatty acids, the levels of butyric acid (C4:0), lauric acid (C12:0), and nervonic acid (C24:1) were lower in the LPR_0.44 group than in the LPR_0.26 group (p < 0.05). No significant differences were observed in intestinal morphology, digestive enzyme activities, or nutrient utilization among the groups. (p > 0.05). In conclusion, adjusting the AM/AP ratio to 0.44 in a low-protein diet improved growth performance, regulated lipid metabolism, and maintained intestinal function in goslings. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

15 pages, 1524 KB

Open AccessArticle

Dynamic Changes in Gut Microbiota Composition and Function over Time in Suckling Raccoon Dogs

by Shaochen Yu, Weixiao Nan, Zhipeng Li, Chongshan Yuan and Chao Xu

Animals 2026, 16(2), 188; https://doi.org/10.3390/ani16020188 - 8 Jan 2026

Abstract

Raccoon dog fur is a commercially valuable animal product. As the scale of raccoon dog breeding continues to expand, ensuring the health of these animals has become an urgent priority. The gut microbiota plays a central role in regulating animal health; however, current [...] Read more.

Raccoon dog fur is a commercially valuable animal product. As the scale of raccoon dog breeding continues to expand, ensuring the health of these animals has become an urgent priority. The gut microbiota plays a central role in regulating animal health; however, current research on the composition of raccoon dog gut microbiota remains limited. This study aimed to characterize changes in the gut microbiota of suckling raccoon dogs across different stages, providing a foundation for future scientific feeding practices. Fecal samples of eight lactating raccoon dogs were collected and tested for microbiota on days 14, 21, and 45. Our results showed that the richness and diversity of microbiota increased with age in suckling raccoon dogs, peaking on the 45th day. Significant separation between groups was observed in both PCoA and NMDS analyses. UPGMA analysis indicated temporal fluctuations in gut microbiota composition. At the phylum level, Firmicutes and Bacteroidetes were the dominant taxa across all stages. LEfSe analysis at the genus level showed that Bacteroides was the most enriched taxon on the 14th day, Fusobacterium on the 21st day, and Prevotella_9 on the 45th day. Tax4Fun and PICRUSt analyses identified metabolism and genetic information processing as the primary functional roles of the gut microbiota. Further investigation suggested that the microbiota may benefit raccoon dogs through membrane transport, carbohydrate metabolism, amino acid metabolism, and energy metabolism. These findings establish a theoretical basis for improving the survival rate of suckling raccoon dogs and developing scientifically informed feeding and management protocols. Full article

(This article belongs to the Special Issue Nutritional Regulation of Gut Microbiota in Animals)

17 pages, 4061 KB

Open AccessArticle

DGS-YOLO: A Detection Network for Rapid Pig Face Recognition

by Hongli Chao, Wenshuang Tu, Tonghe Liu, Hang Zhu, Jinghuan Hu, Tianli Hu, Yu Sun, Ye Mu, Juanjuan Fan and He Gong

Animals 2026, 16(2), 187; https://doi.org/10.3390/ani16020187 - 8 Jan 2026

Abstract

This study addresses the practical demand for facial recognition of pigs in the food safety and insurance industries, tackling the challenge of low recognition accuracy caused by complex farming environments, occlusions, and similar textures. To this end, we propose an enhanced model, DGS-YOLO, [...] Read more.

This study addresses the practical demand for facial recognition of pigs in the food safety and insurance industries, tackling the challenge of low recognition accuracy caused by complex farming environments, occlusions, and similar textures. To this end, we propose an enhanced model, DGS-YOLO, based on YOLOv11n, designed to achieve precise facial recognition of group-raised young pigs. The core improvements of the model include the following: (1) replacing standard convolutions with dynamic convolutions (DMConv) to enhance the network’s adaptive extraction capability for critical detail features; (2) designing a C3k2_GBC module with a bottleneck structure to replace the C3k2 neck, enabling more efficient capture of multi-scale contextual information; (3) introducing the SimAM parameter-free attention mechanism to optimize feature focusing; (4) employing the Shape-IoU loss function to mitigate the impact of bounding box geometry on regression accuracy. Experiments on self-built datasets demonstrate that DGS-YOLO achieves 4%, 2.1%, and 2.3% improvements in accuracy, recall, and mAP50, respectively, compared to the baseline model YOLOv11n. Furthermore, its overall performance surpasses that of Faster R-CNN and SSD in comprehensive evaluation metrics. Especially in limited sample scenarios, the model demonstrates strong generalization ability, with accuracy and mAP50 further increased by 20.1% and 10.3%. This study provides a highly accurate and robust solution for animal facial recognition in complex scenarios. Full article

(This article belongs to the Section Pigs)

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21 pages, 7216 KB

Open AccessArticle

Nest Depth and Height Are Associated with Breeding Outcomes in the Small Bee-Eater (Merops orientalis): A Preliminary Field Study from Pakistan

by Asif Sadam, Muhammad Awais, Huijian Hu, Dongmei Yu and Yiming Hu

Animals 2026, 16(2), 186; https://doi.org/10.3390/ani16020186 - 8 Jan 2026

Abstract

Nest architecture and surrounding habitat features can strongly influence the reproductive success of cavity-nesting birds; however, quantitative data from natural environments remain limited. We examined how nest structure and surrounding habitat features correlate with reproduction in the small bee-eater (Merops orientalis). [...] Read more.

Nest architecture and surrounding habitat features can strongly influence the reproductive success of cavity-nesting birds; however, quantitative data from natural environments remain limited. We examined how nest structure and surrounding habitat features correlate with reproduction in the small bee-eater (Merops orientalis). A total of 38 natural nests were monitored during the breeding season. The Conway–Maxwell–Poisson model showed that cavity depth was a significant positive predictor of clutch size (β = 0.46 ± 0.22 SE, p = 0.036), whereas entrance diameter and nest height were not significantly related. Principal component analysis (PCA) of standardized cavity dimensions (cavity depth, entrance diameter, and nest height) showed that nest height (captured by PC2) was strongly associated with higher breeding success (OR = 0.002, p = 0.021), whereas overall cavity size (PC1) had a weaker, marginally positive correlation (OR = 3.87, p = 0.09). Habitat distance variables showed only weak, non-significant trends after accounting for multicollinearity. Nest structural traits explained more variation in reproductive performance than landscape variables (pseudo-R² = 0.80 for clutch size; 0.59 for breeding success). Field monitoring of 38 nests showed a mean clutch size of 3.9 eggs, an overall hatching success of 77.5%, and a fledging success of 51.2%, yielding a 37.1% breeding success. Our results highlight the importance of conserving sandy streambanks and mitigating human disturbance in proximity to active nests to conserve breeding success in small bee-eaters. As these findings were based on one site and a single breeding season, broader generalizations will require replication across additional years and locations. Full article

(This article belongs to the Section Ecology and Conservation)

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16 pages, 880 KB

Open AccessReview

Factors Affecting Flock Uniformity in Broiler Production: Individual, Environmental, and Management Characteristics

by Janghan Choi, Doyun Goo, Hanseo Ko, Jihwan Lee and Woo Kyun Kim

Animals 2026, 16(2), 185; https://doi.org/10.3390/ani16020185 - 8 Jan 2026

Abstract

Flock uniformity is a critical factor influencing productivity, production efficiency, animal welfare, and overall economic outcomes in broiler production. Despite its importance, uniformity is often overlooked in commercial settings, where production goals typically focus on maximizing average BW and overall flock performance rather [...] Read more.

Flock uniformity is a critical factor influencing productivity, production efficiency, animal welfare, and overall economic outcomes in broiler production. Despite its importance, uniformity is often overlooked in commercial settings, where production goals typically focus on maximizing average BW and overall flock performance rather than minimizing variation among individual birds. This review aims to summarize the diverse factors that affect flock uniformity in broilers. Under standard conditions between days 14 and 49, flock uniformity typically reaches approximately 90%. Both intrinsic factors, such as genetics, initial BW, age, sex, and rearing method, and extrinsic factors, including housing, management, and vaccination practices, have been shown to influence uniformity. Environmental and management challenges, such as heat stress, nutrient deficiencies, microbial infections, high stocking density, feed form, and mixed-sex rearing, can further reduce uniformity by 2.5–15%. These findings highlight the high sensitivity of flock uniformity to multiple stressors, which may interact rather than act independently. Moreover, these findings highlight the need to consider both baseline and challenging factors in broiler production and support the development of targeted management and environmental strategies to improve flock uniformity and enhance overall production efficiency. Full article

(This article belongs to the Section Poultry)

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19 pages, 8261 KB

Open AccessArticle

Pathogenic Characterization and Host Immune Response to Vibrio harveyi in Diseased Seriola dumerili

by Rizhao Zhang, Jingbo Hu, Xin Zhao, Kunpeng Lan, Haimin Tan, Yang Huang and Chunhua Zhu

Animals 2026, 16(2), 184; https://doi.org/10.3390/ani16020184 - 8 Jan 2026

Abstract

The greater amberjack (Seriola dumerili) is an economically important marine species that is prone to bacterial infections, resulting in high mortality rates and substantial economic losses. In this study, a virulent bacterial strain, Vh-2, was isolated from diseased greater amberjack and [...] Read more.

The greater amberjack (Seriola dumerili) is an economically important marine species that is prone to bacterial infections, resulting in high mortality rates and substantial economic losses. In this study, a virulent bacterial strain, Vh-2, was isolated from diseased greater amberjack and identified as Vibrio harveyi. Experimental infections caused high mortality and severe splenic damage characterized by tissue necrosis, abnormal pigment deposition, cellular disintegration, and extensive immune cell infiltration. A virulence gene analysis revealed that Vh-2 harbored multiple virulence-associated genes such as toxR, toxS, vhpA, vhpB, vhhA, vhhB, luxR, and pap6. Antibiotic susceptibility testing demonstrated ampicillin resistance but sensitivity to ceftriaxone, florfenicol, and meropenem. Transcriptomic profiling of infected spleens identified 396 differentially expressed genes (DEGs) compared to the control group, of which 293 were upregulated and 103 were downregulated. A functional enrichment analysis indicated that these genes were primarily involved in cell cycle regulation, DNA repair, metabolic processes, and immune-related pathways. These findings enhance our understanding of V. harveyi pathogenesis and immune responses of S. dumerili and provide new insights into the prevention and control of V. harveyi infections in marine fish. Full article

(This article belongs to the Section Aquatic Animals)

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13 pages, 1779 KB

Open AccessArticle

Climate Change and Biotic Interactions Will Change the Distributions of Ungulates on the Qinghai–Tibet Plateau

by Tong Zhang, Yikai Wang, Fu Shu, Yonglei Lv, Zijun Tang, Feng Liu, Zhiguo Li, Yuan Wang, Guangwei Tang, Guanglong Wang, Nanfei Wu, Keji Guo and Xumao Zhao

Animals 2026, 16(2), 183; https://doi.org/10.3390/ani16020183 - 8 Jan 2026

Abstract

Species interactions are crucial for understanding how species will respond to future climate change. Incorporating interspecific relationships into mammalian distribution prediction models will significantly impact model outcomes, especially those for animals on the Qinghai–Tibet Plateau (QTP). Thus, we incorporated interspecific relationships into species [...] Read more.

Species interactions are crucial for understanding how species will respond to future climate change. Incorporating interspecific relationships into mammalian distribution prediction models will significantly impact model outcomes, especially those for animals on the Qinghai–Tibet Plateau (QTP). Thus, we incorporated interspecific relationships into species distribution models to assess and predict the future distributions of five ungulates, including the Red deer (Cervus elaphus), the Kiang (Equus kiang), the Tibetan gazelle (Procapra picticaudata), the Tibetan antelope (Pantholops hodgsonii), and the Bharal (Pseudois nayaur). We found that (1) the suitable habitats of these five ungulates were all predicted to increase between the present and 2050; (2) the suitable distribution areas of four of these ungulates were predicted to be smaller when interspecific relationships were incorporated into the models, with the exception of the Red deer, whose suitable habitat was estimated to be larger; and (3) the centroids of suitable habitat for the five ungulates were predicted to shift to the southern part of the QTP by 2050. Our results demonstrated that interspecific relationships could influence predictions of species distributions, and thus incorporating interspecific relationships will facilitate better assessments and predictions of the future distributions of species. Full article

(This article belongs to the Section Ecology and Conservation)

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12 pages, 2866 KB

Open AccessFeature PaperArticle

Through the Calf’s Eye: Exploring Infrared Thermography to Uncover Pair-Housed Calves’ Affective States

by Gaia Pesenti Rossi, Sara Barbieri, Emanuela Dalla Costa, Michela Minero and Elisabetta Canali

Animals 2026, 16(2), 182; https://doi.org/10.3390/ani16020182 - 8 Jan 2026

Abstract

Pair housing has been proven to improve calves’ welfare during the pre-weaning phase, potentially promoting positive emotions. Based on the emotional valence hypothesis, according to which positive emotions are mainly processed in the left brain hemisphere, infrared thermography (IRT) may detect changes in [...] Read more.

Pair housing has been proven to improve calves’ welfare during the pre-weaning phase, potentially promoting positive emotions. Based on the emotional valence hypothesis, according to which positive emotions are mainly processed in the left brain hemisphere, infrared thermography (IRT) may detect changes in eye temperature, particularly thermal asymmetries. This study aimed to evaluate whether pair-housed calves presented ocular thermal asymmetry, compared to individually housed calves during the pre-weaning phase. Fifty-six Friesian female calves from two commercial dairy farms were enrolled and housed either individually or in pairs from birth until 8 weeks of age. IRT images of the lacrimal caruncle of both eyes were collected at 7, 21, 35, and 56 days of age. A linear mixed model tested the effects of housing, farm, year, and timepoint. No significant effect of pair housing on ocular asymmetry emerged, while absolute eye temperatures were significantly higher in pair-housed calves. Moreover, eye temperature declined over time, suggesting reduced arousal and habituation with age. Although the lateralization hypothesis was not confirmed, the study offers insights into IRT for assessing calf emotions and supports further investigation in positive contexts to better explore links between housing, emotional valence, and brain activity. Full article

(This article belongs to the Special Issue Infrared Thermography in Animals)

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12 pages, 1875 KB

Open AccessArticle

METTL14 Promotes Lipid Synthesis in Dairy Goat Mammary Epithelial Cells by Targeting CEBPB via m6A-YTHDF1/3-Dependent Manner

by Hongyun Jiao, Lu Zhu, Xinyu Tang, Ping Gong, Wei Wang, Baolong Liu and Jun Luo

Animals 2026, 16(2), 181; https://doi.org/10.3390/ani16020181 - 8 Jan 2026

Abstract

N6-methyladenosine (m6A), a predominant and reversible modification of mammalian RNA, plays a critical role in regulating growth, development, and metabolism. While methyltransferase-like 14 (METTL14) is an essential component of the m6A methyltransferase complex, its specific function in regulating milk fat metabolism in dairy [...] Read more.

N6-methyladenosine (m6A), a predominant and reversible modification of mammalian RNA, plays a critical role in regulating growth, development, and metabolism. While methyltransferase-like 14 (METTL14) is an essential component of the m6A methyltransferase complex, its specific function in regulating milk fat metabolism in dairy goats remains unexplored. This study therefore aimed to elucidate the role of METTL14 in lipid metabolism within dairy goat mammary epithelial cells (GMECs). METTL14 overexpression significantly promoted the synthesis of TAG (Triacylglycerol) and TC (Total cholesterol), as well as lipid droplet accumulation in GMECs. Furthermore, METTL14 upregulated CCAAT enhancer binding protein beta (CEBPB) expression at both the mRNA and protein levels by directly inducing m6A modification on its transcripts. Finally, we confirmed that m6A modification occurs specifically at site 1662 of CEBPB mRNA, and the “Readers” YTH N6-methyladenosine RNA binding protein F1 and F3 (YTHDF1/3) were found responsible for the m6A site recognition and interpretation. This study demonstrated that METTL14 facilitates lipid synthesis and deposition in GMECs. Mechanistically, METTL14 installs the m6A modification at site 1662 of CEBPB transcripts. This m6A mark is specifically recognized by the readers YTHDF1 and YTHDF3, which promote the translation of CEBPB mRNA, thereby upregulating its expression. Full article

(This article belongs to the Section Small Ruminants)

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13 pages, 793 KB

Open AccessArticle

Spatial Ecology of Livestock Protection Dogs, Sheep, and Pampas Foxes in Agroecosystem of Central Argentina

by Sabrina Daniela Martínez, Mauro Lucherini, Nicolás Carmelo Caruso, Emma Beatriz Casanave and Estela Maris Luengos Vidal

Animals 2026, 16(2), 180; https://doi.org/10.3390/ani16020180 - 8 Jan 2026

Abstract

Livestock protection dogs (LPDs) are specifically bred to protect livestock, such as sheep, from predators. These dogs’ primary defense mechanisms include directional actions that deter predators but no attack. Little information is available on the influence of LPDs on the spatial ecology of [...] Read more.

Livestock protection dogs (LPDs) are specifically bred to protect livestock, such as sheep, from predators. These dogs’ primary defense mechanisms include directional actions that deter predators but no attack. Little information is available on the influence of LPDs on the spatial ecology of predators. We analyzed interactions between an LPD, sheep, and Pampas foxes (Lycalopex gymnocercus, a main predator of lambs) in a ranch located in central Argentina. Between 2018 and 2021, we deployed GPS collars on an LPD and 2 ewes from a flock of 300 sheep and VHF collars on 12 live-trapped Pampas foxes. The home ranges (HRs) of the LPD and the ewes overlapped largely, especially during the lambing period, with the LPD performing minimal excursions outside the area used by the sheep flock. The LPD appeared to effectively reduce predation on lambs. Foxes exhibited a large HR (average 6.42 km²), with considerable intraspecific overlap. The overlaps between the HRs of the foxes and the LPD were variable (range = 0–98%), but their respective core areas never overlapped, and the minimum distance between the core area centers was 950 m. This study highlights the effectiveness of LPDs at reducing predation while enabling the permanence of carnivorous predators in the ecosystem. Full article

(This article belongs to the Special Issue Innovative Methods to Reduce Predation and Achieve Coexistence between Predators and Prey)

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24 pages, 17450 KB

Open AccessArticle

Integrated Single-Cell and Bulk Transcriptomics Unveils Immune Profiles in Chick Erythroid Cells upon Avian Pathogenic Escherichia coli Infection

by Fujuan Cai, Xianjue Wang, Chunzhi Wang, Yuzhen Wang and Wenguang Zhang

Animals 2026, 16(2), 179; https://doi.org/10.3390/ani16020179 - 7 Jan 2026

Abstract

Nucleated erythroid cells (NECs) have emerged as active participants in immune responses in addition to their canonical oxygen transport function. The subpopulations and immune heterogeneity of chick erythroid cells (ch-ECs) upon infection have not been fully characterized. Single-cell RNA sequencing (scRNA-seq) was used [...] Read more.

Nucleated erythroid cells (NECs) have emerged as active participants in immune responses in addition to their canonical oxygen transport function. The subpopulations and immune heterogeneity of chick erythroid cells (ch-ECs) upon infection have not been fully characterized. Single-cell RNA sequencing (scRNA-seq) was used to profile ch-ECs in chicks infected with avian pathogenic Escherichia coli (APEC). Unsupervised clustering uncovered ten distinct ch-EC subpopulations (C1–C10), with significant compositional shifts between infected and control groups. Pseudotime analysis revealed a developmental continuum: C1, C3, C5, and C9 as early progenitors; C2, C4, C6, C7, and C10 as mature erythroid cells; and C8 as a naive population. We revealed 62 immune-related genes, including protein kinases and heat shock proteins, and subpopulation-specific differentially expressed genes (DEGs) linked to immune functions. SCENIC analysis revealed Fos, Srf, and Stat3 as key transcription factors with elevated regulon activity and specificity following infection. Subpopulations C2, C4, C6, and C7, which exhibited marked abundance changes, were scrutinized for immune relevance through integrated multi-omics analysis. Immune-related genes including FOS, AKAP9, HS6ST1, GAB3, TFRC, HSPA8, HSP90AA1, and DNAJB6 were identified. Enrichment analysis indicated activation of the MHC class I antigen presentation pathway, while pathways such as Mitogen-Activated Protein Kinase (MAPK) signaling, NOD-like receptor (NLR) signaling, and the heat shock response were found to be suppressed. In conclusion, this study delineates the immune gene repertoire and signaling networks of ch-ECs during APEC infection, offering new perspectives on NEC immunoregulatory functions. Full article

(This article belongs to the Special Issue Bacterial Disease Research in Livestock and Poultry)

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14 pages, 1174 KB

Open AccessArticle

The Effects of Reduced Glutathione on Growth Performance, Intestinal Inflammation, and Gut Microbiota in Immune-Stressed Broiler Chickens

by Xin-Qi Wang, Tao Zhang, Ying-Kun Liu, Hao-Jia Li, Kabelo Anthony Makatjane, Zhen Lai, Jian-Xin Bi, Hai-Zhu Zhou and Wei Guo

Animals 2026, 16(2), 178; https://doi.org/10.3390/ani16020178 - 7 Jan 2026

Abstract

In intensive farming systems, broilers are exposed to various stressors that trigger immune stress. Reduced glutathione is known to play a crucial role in modulating immune function. This study investigated the effects of different doses of reduced glutathione on the growth performance, intestinal [...] Read more.

In intensive farming systems, broilers are exposed to various stressors that trigger immune stress. Reduced glutathione is known to play a crucial role in modulating immune function. This study investigated the effects of different doses of reduced glutathione on the growth performance, intestinal immune function, and gut microbiota of broilers under immune stress. One-day-old broilers were randomly assigned to five groups: group K (control), group L (lipopolysaccharide), and three glutathione supplementation groups, Y1 (50 mg/kg), Y2 (100 mg/kg), and Y3 (200 mg/kg). Each group had four replicates, with 11 broilers per replicate. On days 16, 18, and 20, broilers in groups L and Y1-Y3 received intraperitoneal injections of 0.5 mg/kg of lipopolysaccharide, while group K received an equal volume of saline. On day 16, the average daily weight gain and final body weight of group Y3 were significantly higher than those of groups K and L, whereas on day 21, the average daily weight gain of group Y3 was still significantly higher than that of group L. On day 21 broilers were slaughtered, and samples were collected. In the jejunal mucosa, group Y3 showed significantly reduced levels of IL-2, IL-4, IL-1β, and TNF-α compared to group L. Additionally, group Y3 exhibited reduced relative expression levels of NF-κB, TLR4, IFN-γ, IL-1β, IL-6, IL-4, TNF-α, and IL-2 mRNA; a decreased abundance of Enterococcus, Proteobacteria, and Actinobacteria; and a restored abundance of Ligilactobacillus, Turicibacter, and Firmicutes to normal levels. These findings suggest that 200 mg/kg of reduced glutathione is the optimal dose for improving the gut microbiota composition and mitigating gut damage caused by immune stress by inhibiting the TLR4/NF-κB signaling pathway. Full article

(This article belongs to the Special Issue Feed Additives in Animal Nutrition)

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21 pages, 1866 KB

Open AccessArticle

Lean DLY Pig-Derived Fecal Microbiota Promotes Growth Performance by Modulating Gut Microbiota: Serum Metabolic Profiles in Obese Ningxiang Pigs

by Li Han, Feng Zhou, Chen Zhang, Hongkun Li, Yongmin Zheng, Yv Tian, Yang Liu, Jie Yin and Xingguo Huang

Animals 2026, 16(2), 177; https://doi.org/10.3390/ani16020177 - 7 Jan 2026

Abstract

Fecal microbiota transplantation (FMT) has demonstrated potential in reshaping gut microbiota to improve animal phenotypes, yet its application in lean-type to obese-type pigs like Ningxiang (NX) pigs remains unclear. To address this, we investigated the effects of Lean Duroc × Landrace × Yorkshire [...] Read more.

Fecal microbiota transplantation (FMT) has demonstrated potential in reshaping gut microbiota to improve animal phenotypes, yet its application in lean-type to obese-type pigs like Ningxiang (NX) pigs remains unclear. To address this, we investigated the effects of Lean Duroc × Landrace × Yorkshire (DLY) pig-derived fecal microbiota on the growth, gut microbiota composition, and serum metabolism of obese NX pigs. Thirty-six 50-day-old castrated male NX pigs of similar initial body weight were randomly assigned to either a control group or FMT group. The trial lasted for 35 days. Results indicated that FMT significantly improved the average daily gain and increased nutrient digestibility. Serum biochemical analysis revealed elevated levels of globulin and total protein and reduced low-density lipoprotein cholesterol in the FMT group. In addition, 16S rRNA sequencing demonstrated that FMT modified gut microbiota composition and diversity, enriching beneficial genera such as Blautia, Agathobacter, Faecalibacterium, and Eubacterium_coprostanoligenes_group. Untargeted serum metabolomics further revealed altered metabolite profiles linked to lipid and amino acid metabolism. Correlation analysis further revealed a link between these enriched bacteria and metabolites changes. Overall, these findings demonstrate that transplantation of the fecal microbiota from lean DLY pigs significantly improved the growth performance of obese NX pigs by improving nutrient digestibility and modulating the gut microbiota–host metabolic axis. Full article

(This article belongs to the Section Pigs)

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