Biology

17 pages, 6632 KiB

Open AccessArticle

Molecular Docking of Key Compounds from Acacia Honey and Nigella sativa Oil and Experimental Validation for Colitis Treatment in Albino Mice

by Mehwish Bibi, Irfan Baboo, Hamid Majeed, Santosh Kumar and Maximilian Lackner

Biology 2024, 13(12), 1035; https://doi.org/10.3390/biology13121035 - 11 Dec 2024

Abstract

Colitis, an inflammatory condition of the colon that encompasses ulcerative colitis (UC) and Crohn’s disease, presents significant challenges due to the limitations and side effects of current treatments. This study investigates the potential of natural products, specifically AH and NSO, as organic therapeutic [...] Read more.

Colitis, an inflammatory condition of the colon that encompasses ulcerative colitis (UC) and Crohn’s disease, presents significant challenges due to the limitations and side effects of current treatments. This study investigates the potential of natural products, specifically AH and NSO, as organic therapeutic agents for colitis. Molecular docking studies were conducted to identify the binding affinities and interaction mechanisms between the bioactive compounds in AH and NSO and proteins implicated in colitis, such as those involved in inflammation and oxidative stress pathways. An in vivo experiment was performed using an albino mouse model of colitis, with clinical symptoms, histopathological assessments, and biochemical analyses conducted to evaluate the therapeutic effects of the compounds both individually and in combination. Results from the molecular docking studies revealed promising binding interactions between fructose and Prostaglandin G/H synthase 2 (Ptgs2) and between fructose and cellular tumor antigen p53, with docking energy measured at −6.0 kcal/mol and −5.1 kcal/mol, respectively. Meanwhile, the presence of glucose molecule glucokinase chain A (−6.3 kcal/mol) and chain B (−5.8 kcal/mol) indicated potential efficacy in modulating inflammatory pathways. Experimental data demonstrated that treatment with AH and NSO significantly reduced inflammation, improved gut health, and ameliorated colitis symptoms. Histopathological evaluations confirmed reduced mucosal damage and immune cell infiltration, while biochemical analyses showed normalization of inflammatory markers and oxidative stress levels. This study provides compelling evidence for the potential of AH and NSO as natural, complementary treatments for colitis, suggesting their future role in integrative therapeutic strategies. However, further research into long-term safety, optimal dosing, and mechanisms of action is warranted to translate these findings into clinical applications. Full article

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15 pages, 1689 KiB

Open AccessArticle

Identifying Significant SNPs of the Total Number of Piglets Born and Their Relationship with Leg Bumps in Pigs

by Siroj Bakoev, Lyubov Getmantseva, Maria Kolosova, Faridun Bakoev, Anatoly Kolosov, Elena Romanets, Varvara Shevtsova, Timofey Romanets, Yury Kolosov and Alexander Usatov

Biology 2024, 13(12), 1034; https://doi.org/10.3390/biology13121034 - 11 Dec 2024

Abstract

The aim of this study was to identify genetic variants and pathways associated with the total number of piglets born and to investigate the potential negative consequences of the intensive selection for reproductive traits, particularly the formation of bumps on the legs of [...] Read more.

The aim of this study was to identify genetic variants and pathways associated with the total number of piglets born and to investigate the potential negative consequences of the intensive selection for reproductive traits, particularly the formation of bumps on the legs of pigs. We used genome-wide association analysis and methods for identifying selection signatures. As a result, 47 SNPs were identified, localized in genes that play a significant role during sow pregnancy. These genes are involved in follicle growth and development (SGC), early embryonic development (CCDC3, LRRC8C, LRFN3, TNFRSF19), endometrial receptivity and implantation (NEBL), placentation, and embryonic development (ESRRG, GHRHR, TUSC3, NBAS). Several genes are associated with disorders of the nervous system and brain development (BCL11B, CDNF, ULK4, CC2D2A, KCNK2). Additionally, six SNPs are associated with the formation of bumps on the legs of pigs. These variants include intronic variants in the CCDC3, ULK4, and MINDY4 genes, as well as intergenic variants, regulatory region variants, and variants in the exons of non-coding transcripts. The results suggest important biological pathways and genetic variants associated with sow fertility and highlight the potential negative impacts on the health and physical condition of pigs. Full article

(This article belongs to the Special Issue Reproductive Physiology and Pathology in Livestock)

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17 pages, 1673 KiB

Open AccessReview

ROS, an Important Plant Growth Regulator in Root Growth and Development: Functional Genes and Mechanism

by Jialin Su, Yumei Liu, Fengqing Han, Fuxin Gao, Fangyi Gan, Ke Huang and Zhansheng Li

Biology 2024, 13(12), 1033; https://doi.org/10.3390/biology13121033 - 10 Dec 2024

Abstract

Roots are fundamental to the growth, development, and survival of plants. Beyond anchoring the plant, roots absorb water and nutrients, supporting the plant’s ability to grow and function normally. Root systems, originating from the apical meristem, exhibit significant diversity depending on the plant [...] Read more.

Roots are fundamental to the growth, development, and survival of plants. Beyond anchoring the plant, roots absorb water and nutrients, supporting the plant’s ability to grow and function normally. Root systems, originating from the apical meristem, exhibit significant diversity depending on the plant species. ROS are byproducts of aerobic metabolism, present in both above- and below-ground plant tissues. While ROS were once considered merely harmful byproducts of oxygen metabolism, they are now recognized as critical signaling molecules that regulate plant growth and development. Under stress conditions, plants produce elevated levels of ROS, which can inhibit growth. However, moderate ROS levels act as signals that integrate various regulatory pathways, contributing to normal plant development. However, there is still a lack of comprehensive and systematic research on how ROS precisely regulate root growth and development. This review provides an overview of ROS production pathways and their regulatory mechanisms in plants, with a particular focus on their influence on root development. Full article

(This article belongs to the Special Issue Unraveling the Molecular Mechanisms of Development and Stress Tolerance in Vegetables)

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19 pages, 3613 KiB

Open AccessArticle

Sexual Body Size Dimorphism in Small Mammals: A Case Study from Lithuania

by Linas Balčiauskas and Laima Balčiauskienė

Biology 2024, 13(12), 1032; https://doi.org/10.3390/biology13121032 - 10 Dec 2024

Abstract

We assessed the sexual size dimorphism (SSD), analyzing standard morphometric traits in juveniles, subadults, and adults, of 14 species of voles, mice, and shrews in Lithuania on the basis of long-term surveys, updating information published 35 years ago and in the context of [...] Read more.

We assessed the sexual size dimorphism (SSD), analyzing standard morphometric traits in juveniles, subadults, and adults, of 14 species of voles, mice, and shrews in Lithuania on the basis of long-term surveys, updating information published 35 years ago and in the context of data from other countries. ANOVA, t-tests, and a 5% threshold were used in the analyses. Male-biased SSD was observed in Apodemus flavicollis and Alexandromys oeconomus, which was subject to Rensch’s rule, and in three other meadow vole species, with the strongest expression in adult individuals. Female-biased SSD was best expressed in Clethrionomys glareolus and was also identified in four mouse species, although patterns were less consistent across age groups. Shrews and the smallest mouse, Apodemus uralensis, exhibited monomorphic traits. The transition of dimorphism during postnatal development was observed in some species. We present an assessment of SSD in Microtus rossiaemeridionalis and Sicista betulina, the less common small mammals not analyzed in publications by other authors, and provide new baseline data for boreal mid-latitude small mammal species as a foundation for future studies on SSD and its adaptive significance under shifting ecological pressures or climate change. Full article

(This article belongs to the Special Issue Advances in Wildlife Conservation and Habitat Management in the Anthropocene)

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15 pages, 2261 KiB

Open AccessFeature PaperArticle

Circadian Rhythms of Skin Surface Lipids and Physiological Parameters in Healthy Chinese Women Reveals Circadian Changes in Skin Barrier Function

by Lanxing Lv, Xiaoxi Yan, Mingyue Zhou, Huaming He and Yan Jia

Biology 2024, 13(12), 1031; https://doi.org/10.3390/biology13121031 - 10 Dec 2024

Abstract

Background: Circadian rhythms are driven by the biological clock, an endogenous oscillator that generates approximately 24 h cycles in mammals. The circadian regulation of the lipid metabolism plays a crucial role in overall metabolic health. An analysis of the correlation between the skin’s [...] Read more.

Background: Circadian rhythms are driven by the biological clock, an endogenous oscillator that generates approximately 24 h cycles in mammals. The circadian regulation of the lipid metabolism plays a crucial role in overall metabolic health. An analysis of the correlation between the skin’s physiological parameters and skin lipids can provide a better insight into the rhythmic changes in skin condition. Objectives: The aim was to reveal how skin surface lipids (SSLs) participate in the regulation of circadian rhythms in the skin and the importance of the circadian oscillation of facial lipid molecules in maintaining epidermal homeostasis. Methods: Changes in SSLs were assessed using UPLC-QTOF-MS. The skin’s physiological parameters were quantified using non-invasive instruments. Multivariate data analysis was employed to evaluate the differences. Results: Both skin surface lipids and physiological parameters exhibited certain circadian variation patterns. Four major lipid classes (fatty acids, glycerophospholipids, prenol lipids, saccharolipids) exhibited circadian rhythmic trends, with seven lipid subclasses contributing most significantly to the overall patterns observed. Among the physiological parameters assessed, sebum secretion, transepidermal water loss, moisture measurement value, and skin surface temperature exhibited sinusoidal circadian rhythms. Further analysis revealed significant correlations between fatty acids and saccharolipids with moisture measurement values, and between glycerolipids and pH value. In addition, lipids closely associated with the barrier such as unsaturated fatty acids and ceramide chain lengths correlated significantly with moisture measurement values. Conclusions: Through correlation analysis, the study elucidates the influence of diurnal fluctuations in skin surface lipids on skin barrier function. These findings hold significant implications for understanding skin barrier impairment associated with circadian rhythm disruptions. Full article

(This article belongs to the Section Medical Biology)

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19 pages, 1126 KiB

Open AccessReview

Retinal Inflammation and Reactive Müller Cells: Neurotrophins’ Release and Neuroprotective Strategies

by Bijorn Omar Balzamino, Andrea Cacciamani, Lucia Dinice, Michela Cecere, Francesca Romana Pesci, Guido Ripandelli and Alessandra Micera

Biology 2024, 13(12), 1030; https://doi.org/10.3390/biology13121030 - 9 Dec 2024

Abstract

Millions of people worldwide suffer from retinal disorders. Retinal diseases require prompt attention to restore function or reduce progressive impairments. Genetics, epigenetics, life-styling/quality and external environmental factors may contribute to developing retinal diseases. In the physiological retina, some glial cell types sustain neuron [...] Read more.

Millions of people worldwide suffer from retinal disorders. Retinal diseases require prompt attention to restore function or reduce progressive impairments. Genetics, epigenetics, life-styling/quality and external environmental factors may contribute to developing retinal diseases. In the physiological retina, some glial cell types sustain neuron activities by guaranteeing ion homeostasis and allowing effective interaction in synaptic transmission. Upon insults, glial cells interact with neuronal and the other non-neuronal retinal cells, at least in part counteracting the biomolecular changes that may trigger retinal complications and vision loss. Several epigenetic and oxidative stress mechanisms are quickly activated to release factors that in concert with growth, fibrogenic and angiogenic factors can influence the overall microenvironment and cell-to-cell response. Reactive Müller cells participate by secreting neurotrophic/growth/angiogenic factors, cytokines/chemokines, cytotoxic/stress molecules and neurogenic inflammation peptides. Any attempt to maintain/restore the physiological condition can be interrupted by perpetuating insults, vascular dysfunction and neurodegeneration. Herein, we critically revise the current knowledge on the cell-to-cell and cell-to-mediator interplay between Müller cells, astrocytes and microglia, with respect to pro-con modulators and neuroprotective/detrimental activities, as observed by using experimental models or analyzing ocular fluids, altogether contributing a new point of view to the field of research on precision medicine. Full article

(This article belongs to the Section Cell Biology)

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19 pages, 5207 KiB

Open AccessArticle

Characteristic Muscle Quality Parameters of Male Largemouth Bass (Micropterus salmoides) Distinguished from Female and Physiological Variations Revealed by Transcriptome Profiling

by Qingchun Wang, Siqi Lu, Yifan Tao, Jixiang Hua, Yan Zhuge, Wenhua Chen and Jun Qiang

Biology 2024, 13(12), 1029; https://doi.org/10.3390/biology13121029 - 8 Dec 2024

Abstract

Male largemouth bass (Micropterus salmoides) are often overlooked because females grow faster. We explored the value of male largemouth bass by comparing muscle nutrition, texture, and transcriptomes between males and females. Females grew faster than males (p < 0.05) because [...] Read more.

Male largemouth bass (Micropterus salmoides) are often overlooked because females grow faster. We explored the value of male largemouth bass by comparing muscle nutrition, texture, and transcriptomes between males and females. Females grew faster than males (p < 0.05) because of lipid accumulation. Male fish muscles had higher contents of serine, valine, methionine, arginine, nervonic acid, and α-linolenic acid (p < 0.05), and female fish muscles had higher contents of aspartic acid, glycine, cysteine, leucine, palmitic acid, docosahexaenoic acid, and 11,14,17-eicosatrienoic acid (p < 0.05). Male muscles had a higher concentration of collagen fibers and greater shear force, indicative of a chewier texture. Male muscles had a lighter color, suggesting that they were less susceptible to oxidation and deterioration. Transcriptomic analyses revealed upregulation of lpl, sadb, dgat2, bhmt, tecrb, and hsd3b7, encoding components of amino acid and fatty acid metabolism; and upregulation of akt2, src, and kras, encoding crucial regulators of cellular immunity and homeostasis, in male muscles. Immunity-related pathways, including apoptosis, ErbB signaling, and cellular senescence, were enriched in male fish muscles, indicating heightened immune function. The muscles of male fish have a unique profile and distinctive advantages in terms of nutrition, flavor, texture, and transcriptional regulation. Full article

(This article belongs to the Section Physiology)

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18 pages, 3255 KiB

Open AccessArticle

Nicotinamide Mononucleotide Alleviates Bile Acid Metabolism and Hormonal Dysregulation in Letrozole-Induced PCOS Mice

by Caifang Ren, Shuang Zhang, Jianyu Ma, Junjie Huang, Pan Huang, Mingzi Qu, Haoyue Zhao, Zhengrong Zhou and Aihua Gong

Biology 2024, 13(12), 1028; https://doi.org/10.3390/biology13121028 - 8 Dec 2024

Abstract

Polycystic ovary syndrome (PCOS) involves complex genetic, metabolic, endocrine, and environmental factors. This study explores the effects of nicotinamide mononucleotide (NMN) in a letrozole-induced PCOS mouse model, focusing on metabolic regulation. Letrozole-induced aromatase inhibition elevated androgen and reduced bile acid levels, linking liver [...] Read more.

Polycystic ovary syndrome (PCOS) involves complex genetic, metabolic, endocrine, and environmental factors. This study explores the effects of nicotinamide mononucleotide (NMN) in a letrozole-induced PCOS mouse model, focusing on metabolic regulation. Letrozole-induced aromatase inhibition elevated androgen and reduced bile acid levels, linking liver dysfunction and gut imbalance to PCOS. Letrozole-treated mice exhibited disrupted estrous cycles, ovarian congestion, and elevated testosterone. NMN intervention alleviated hyperandrogenism, ovarian abnormalities, and bile acid decline but did not fully restore the estrous cycle or improve lipid profiles. Metabolomic analysis showed that NMN partially reversed bile acid and lipid metabolism disturbances. These findings highlight NMN’s protective role in reducing hyperandrogenism and ovarian cyst formation. However, effective PCOS treatment should target liver and gut metabolism, not just ovarian symptoms, to mitigate systemic effects. Bile acid dysregulation may play a key role in PCOS progression and warrants further investigation. Full article

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Graphical abstract

19 pages, 4540 KiB

Open AccessArticle

Peroxisome Proliferator Activator α Agonist Clofibrate Induces Pexophagy in Coconut Oil-Based High-Fat Diet-Fed Rats

by Kanami Ohshima, Emika Hara, Mio Takimoto, Yidan Bai, Mai Hirata, Wen Zeng, Suzuka Uomoto, Mai Todoroki, Mio Kobayashi, Takuma Kozono, Tetsuhito Kigata, Makoto Shibutani and Toshinori Yoshida

Biology 2024, 13(12), 1027; https://doi.org/10.3390/biology13121027 - 7 Dec 2024

Abstract

Peroxisomes are crucial for fatty acid β-oxidation in steatosis, but the role of pexophagy—the selective autophagy of peroxisomes—remains unclear. This study investigated the effects of the peroxisome proliferator-activated receptor-α (PPARα) agonist clofibrate on pexophagy in a coconut oil-based high-fat diet (HFD)-induced hepatocarcinogenesis model. [...] Read more.

Peroxisomes are crucial for fatty acid β-oxidation in steatosis, but the role of pexophagy—the selective autophagy of peroxisomes—remains unclear. This study investigated the effects of the peroxisome proliferator-activated receptor-α (PPARα) agonist clofibrate on pexophagy in a coconut oil-based high-fat diet (HFD)-induced hepatocarcinogenesis model. Rats were divided into four groups: control, clofibrate, HFD, and HFD with clofibrate. The HFD induced steatosis, along with a 2.4-fold increase in pexophagy receptor NBR1-positive granules in hepatocytes. Clofibrate significantly inhibited HFD-induced steatosis, increasing p62-, LAMP2-, and Pex5-positive granules by 7.5-, 7.2-, and 71.4-fold, respectively, while decreasing NBR1 expression. The effects were associated with peroxisome proliferation and pexophagy in ultrastructural observations and increased levels of Lc3, p62, Pex2, Pex14, Acox1, and Scd1 in gene expression analysis. The results suggested that clofibrate effectively reduced steatosis through combined peroxisome proliferation and pexophagy, though it had a marginal impact on hepatocarcinogenesis in coconut oil-based HFD-fed rats. These findings highlight the utility of PPARα agonists in studying mammalian pexophagy. Full article

(This article belongs to the Special Issue Animal Models in Toxicology)

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11 pages, 948 KiB

Open AccessArticle

Geographic Variation in Signal Preferences in the Tropical Katydid Neoconocephalus triops

by Oliver M. Beckers and Johannes Schul

Biology 2024, 13(12), 1026; https://doi.org/10.3390/biology13121026 - 7 Dec 2024

Abstract

In communication systems, the signal and preference for the signal have to match, limiting phenotypic variation. Yet, communication systems evolve, but the mechanisms of how phenotypic variation can come into existence while not disrupting the match are poorly understood. Geographic variation in communication [...] Read more.

In communication systems, the signal and preference for the signal have to match, limiting phenotypic variation. Yet, communication systems evolve, but the mechanisms of how phenotypic variation can come into existence while not disrupting the match are poorly understood. Geographic variation in communication can provide insights into the diversification of these systems. Females of the katydid Neoconocephalus triops use the pulse rate and call structure for call recognition. Using behavioral experiments, we determined preferences for pulse rate at two relevant ambient temperatures and preferences for call structure (continuous, versed) in females from Puerto Rico and Costa Rica. Puerto Rican females had closed preference at both tested temperatures, indicating high selectivity for pulse rate. In contrast, Costa Rican females had a closed preference only at 20 °C; at 25 °C the females were unselective toward higher than natural pulse rates. Additionally, Puerto Rican females were not selective for call structure, whereas Costa Rican females preferred versed calls. It is not clear whether the differences in pulse preference were due to neural constraints or different selective pressures, however, they may facilitate further divergence and reproductive isolation. Importantly, the reduced selectivity for call structure or pulse rate allows calls to display the necessary variation for the communication system to evolve. Full article

(This article belongs to the Section Zoology)

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3 pages, 171 KiB

Open AccessEditorial

Biology: The Open Road to a Theory of Life

by Andrés Moya and Jukka Finne

Biology 2024, 13(12), 1025; https://doi.org/10.3390/biology13121025 - 7 Dec 2024

Abstract

The journal Biology was launched in 2012 [...] Full article

19 pages, 1316 KiB

Open AccessReview

The Protein Corona on Nanoparticles for Tumor Targeting in Prostate Cancer—A Review of the Literature and Experimental Trial Protocol

by Giulio Bevilacqua, Roberta Corvino, Anna Laura Capriotti, Carmela Maria Montone, Martina Moriconi, Stefano Salciccia, Valentina Brunelli, Valerio Santarelli, Beatrice Sciarra, Aldo Laganà, Daniele Santini, Alessandro Sciarra and Alessandro Gentilucci

Biology 2024, 13(12), 1024; https://doi.org/10.3390/biology13121024 - 7 Dec 2024

Abstract

The National Cancer Institute (NCI) recognizes the potential of technologies based on the use of nanoparticles (NPs) in revolutionizing clinical approaches to the diagnosis and prognosis of cancer. Recent research suggests that once NPs come into contact with the biological fluid of cancer [...] Read more.

The National Cancer Institute (NCI) recognizes the potential of technologies based on the use of nanoparticles (NPs) in revolutionizing clinical approaches to the diagnosis and prognosis of cancer. Recent research suggests that once NPs come into contact with the biological fluid of cancer patients, they are covered by proteins, forming a “protein corona” composed of hundreds of plasma proteins. The concept of a personalized, disease-specific protein corona, demonstrating substantial differences in NP corona profiles between patients with and without cancer, has been introduced. We developed the design of an experimental prospective single-center study with patients allocated in a 1:1:1 ratio of one of three arms: untreated patients with benign prostatic hyperplasia (BPH), untreated patients with non-metastatic prostate cancer (PCa), and metastatic prostate cancer patients starting systemic therapies with new androgen-targeted agents or taxanes. The protocol aims to develop and implement sensitive nanotools with two distinct objectives: First, to design NPs capable of selectively binding and detecting biomarkers in order to build a predictive diagnostic model to effectively discriminate between patient sera affected by BPH and PCa. Secondly, within the population with PCa, in the case of initial advanced metastatic diagnosis, the objective is to find biomarkers capable of predicting the response to systemic treatments to improve the precision and efficiency of monitoring treatment outcomes. For protein and metabolite corona experiments, we developed a cross-reactive sensor array platform with cancer detection capacity made of three liposomal formulations with different surface charges. For proteomic-NP studies, proteins were identified and quantified using nano-high-performance LC (nanoHPLC) coupled with MS/MS (nanoHPLC−MS/MS). Metabolites were instead analyzed using an untargeted metabolomic approach. Compared with previous review articles, the novelty of this review is represented by the analysis of the possible clinical applications of protein corona NPs focused on PCa and the presentation of a new clinical protocol in the metastatic phase of PCa. Full article

(This article belongs to the Special Issue New Sight in Cancer Genetics)

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20 pages, 1969 KiB

Open AccessArticle

Trophic Structure and Isotopic Niche of Invaded Benthic Communities on Tropical Rocky Shores

by Larissa M. Pires-Teixeira, Vinicius Neres-Lima, Plínio C. Barbosa and Joel C. Creed

Biology 2024, 13(12), 1023; https://doi.org/10.3390/biology13121023 - 7 Dec 2024

Abstract

When a species is introduced in a new location, it is common for it to establish itself when it finds favorable conditions in the receptor community with regard to interspecific interactions with native species. The azooxanthellate corals Tubastraea coccinea and Tubastraea tagusensis are invasive [...] Read more.

When a species is introduced in a new location, it is common for it to establish itself when it finds favorable conditions in the receptor community with regard to interspecific interactions with native species. The azooxanthellate corals Tubastraea coccinea and Tubastraea tagusensis are invasive species introduced in the Caribbean Sea, the Gulf of Mexico, and the Brazilian Southwest Atlantic. They are successful competitors for space, have multiple reproductive modes, and have high larval dispersion and recruitment, but studies on food and trophic relationships of the genus Tubastraea are still scarce. In the present study, we used isotopic values of δ¹³C and δ¹⁵N to investigate trophic relationships in rocky shore communities invaded by T. tagusensis and T. coccinea corals under different oceanographic and anthropogenic contexts. Using metrics derived from the isotopic values, we show that invaded communities have a lower degree of trophic diversity, with species characterized by similar trophic ecologies while abiotic factors seem to contribute to the biotic resistance of communities exposed to invasion events. Tubastraea spp. occupy a niche space similar to that occupied by the native community of suspension feeders, sharing resources already consumed by the receptor community, which makes invading corals successful competitors for food. Full article

(This article belongs to the Special Issue 2nd Edition: Aquatic Alien Invasions and Their Impact on Biodiversity and Ecosystem Services )

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19 pages, 4474 KiB

Open AccessArticle

Antibiotic Resistance as a Functional Characteristic of Urban Dust Particles’ Microbial Communities

by Anna A. Vetrova, Anastasia A. Ivanova, Kirill V. Petrikov, Olga Gavrichkova, Maria V. Korneykova and Olesya I. Sazonova

Biology 2024, 13(12), 1022; https://doi.org/10.3390/biology13121022 - 6 Dec 2024

Abstract

Urban dust samples were collected in Moscow (Russia) in June 2021. The samples were collected in three functional zones of Moscow (traffic, residential, and recreational) and included air microparticles, leaf dust, and paved dust. Data on the taxonomic composition of bacterial communities were [...] Read more.

Urban dust samples were collected in Moscow (Russia) in June 2021. The samples were collected in three functional zones of Moscow (traffic, residential, and recreational) and included air microparticles, leaf dust, and paved dust. Data on the taxonomic composition of bacterial communities were obtained for dust samples, and their functional characteristics were predicted using PICRUSt2 2.5.0 and FAPROTAX 1.8.0 software. The culturable part of the bacterial community was examined for the presence of antibiotic-resistant strains with respect to β-lactams, tetracyclines, amphenicols, and aminoglycosides. The presence of bacteria resistant to ceftazidime, cefepime, and tetracycline was detected in all dust samples. The presence of bacteria resistant to meropenem and amikacin was only observed in the dust collected from leaves in the residential and traffic zones. The overall abundance of cultured antibiotic-resistant bacteria from the total heterotrophs ranged from 0.03% to 1.88%, with the highest percentage observed in dust from the residential zone. Notably, strains resistant to all antibiotics tested were observed in the leaf dust bacterial community. Full article

(This article belongs to the Special Issue Adaptation of Living Species to Environmental Stress)

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20 pages, 2644 KiB

Open AccessArticle

Intelligibility Sound Therapy Enhances the Ability of Speech-in-Noise Perception and Pre-Perceptual Neurophysiological Response

by Takashi Ishino, Kei Nakagawa, Fumiko Higashikawa, Sakura Hirokane, Rikuto Fujita, Chie Ishikawa, Tomohiro Kawasumi, Kota Takemoto, Takashi Oda, Manabu Nishida, Yuichiro Horibe, Nobuyuki Chikuie, Takayuki Taruya, Takao Hamamoto, Tsutomu Ueda, Louis Yuge and Sachio Takeno

Biology 2024, 13(12), 1021; https://doi.org/10.3390/biology13121021 - 6 Dec 2024

Abstract

Aural rehabilitation with hearing aids can decrease the attentional requirements of cognitive resources by amplifying deteriorated-frequency sound in hearing loss patients and improving auditory discrimination ability like speech-in-noise perception. As aural rehabilitation with an intelligible-hearing sound also can be hopeful, the aim of [...] Read more.

Aural rehabilitation with hearing aids can decrease the attentional requirements of cognitive resources by amplifying deteriorated-frequency sound in hearing loss patients and improving auditory discrimination ability like speech-in-noise perception. As aural rehabilitation with an intelligible-hearing sound also can be hopeful, the aim of this study was to evaluate the effectiveness of aural rehabilitation with intelligible-hearing sound for hearing loss patients. Adult native Japanese speakers (17 males and 23 females, 68.43 ± 9.23 years) with hearing thresholds exceeding 30 dB at any of the following frequencies: 125, 250, 500, 1000, 2000, 3000, 4000, 8000, 10,000, and 12,000 Hz in either ear, were recruited. on any side were recruited and underwent the Mini-Mental State Examination Japanese. We conducted a self-evaluation questionnaire for hearing problems of voice, a gap detection test, a fast speech test, a speech-in-noise test, a pure tone audiogram, and a speech perception test using a Japanese 67-S, cortical auditory-evoked fields, and magnetic mismatch negativity before and after the non-intelligible-hearing (N = 20) and intelligible-hearing (N = 20) sound therapy, which involved listening to music for one hour a day for 35 days. The better hearing ear was defined using a four-frequency pure-tone average at the thresholds of 500, 1000, 2000, and 4000 Hz. After the sound therapy, the speech-in-noise test with a signal-to-noise ratio +10 in the better hearing ear showed significant improvement (p < 0.05), and N1m-P2m amplitudes showed a significant increase in the Lt superior temporal gyrus in response to the stimulus from the better hearing ear (p < 0.05). A significant enhancement of the magnetic mismatch negativity amplitude at the Lt superior temporal gyrus was exhibited after the sound therapy (p < 0.01). Intelligible-hearing sound therapy can improve the ability of speech-in-noise perception in the better hearing ear and enhancement of central cortex response, which reflects the ability of working memory, was proved by cortical auditory-evoked fields and magnetic mismatch negativity. Intelligible-hearing sound therapy can be a valuable aural rehabilitation method for sensory neural hearing loss, the same as hearing aids. Full article

(This article belongs to the Section Neuroscience)

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17 pages, 14193 KiB

Open AccessReview

Research Progress in Biodiversity and Human Well-Being, Based on CiteSpace

by Sunbowen Zhang, Linsheng Wen, Aifang Weng, Dongliang Cheng and Baoyin Li

Biology 2024, 13(12), 1020; https://doi.org/10.3390/biology13121020 - 6 Dec 2024

Abstract

Biodiversity is crucial for human well-being and economic prosperity, representing a significant factor in constructing a global community of life on Earth. This study focuses on 4453 articles indexed in the Web of Science Core Collection, employing the visualization analysis software Citespace 6.2.R7 [...] Read more.

Biodiversity is crucial for human well-being and economic prosperity, representing a significant factor in constructing a global community of life on Earth. This study focuses on 4453 articles indexed in the Web of Science Core Collection, employing the visualization analysis software Citespace 6.2.R7 for bibliometric analysis of research related to biodiversity and human well-being. The findings indicate the following: (1) there is a steady increase in publications on biodiversity and human well-being, covering 167 disciplinary fields, indicating a broad research scope; (2) the literature spans 168 countries and regions, with major contributions from the United States, England, Germany, Australia, and China; (3) COSTANZA R is the most cited author in this field, with Science being the most recognized journal; and (4) current research hot topics include ecosystem services, genetic diversity, marine protected areas, nature-based solutions (NbS), and green spaces, while climate change, urban green space management, and national park system development are identified as important emerging directions. This study, grounded in significant global literature, aims to enrich the breadth and depth of research on biodiversity and human well-being, offering feasibility references for constructing theoretical frameworks in biodiversity studies and providing theoretical guidance for promoting modern harmonious coexistence between humans and nature. Full article

(This article belongs to the Section Conservation Biology and Biodiversity)

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14 pages, 2008 KiB

Open AccessArticle

Contemporary Survival Selection Fails to Explain Observed Patterns of Phenotypic Divergence Between Suburban and Forest Populations of the Cuban Endemic Lizard, Anolis homolechis

by Annabelle Vidal, Frank Cézilly and Roger Pradel

Biology 2024, 13(12), 1019; https://doi.org/10.3390/biology13121019 - 5 Dec 2024

Abstract

Although urbanization is a major threat to biodiversity, some native species have managed to persist in urban areas. Populations of such species often show phenotypic differences with their rural counterparts. A crucial question is whether such differences result from different selection regimes between [...] Read more.

Although urbanization is a major threat to biodiversity, some native species have managed to persist in urban areas. Populations of such species often show phenotypic differences with their rural counterparts. A crucial question is whether such differences result from different selection regimes between habitats. As previous studies showed that suburban and forest populations of Anolis homolechis differ in both body size and sex ratio, we tested the effect of urbanization on adult survival in those populations based on a long-term capture–marking–recapture survey and replicated design. We assessed the evidence for directional and stabilizing selection on size in two suburban and two forest populations by modeling apparent adult survival and recapture probability separately. Adult survival did not differ between habitat types. In addition, there was no evidence for size-related differential selection on adult survival between habitats. However, irrespective of habitat, we found significant stabilizing selection on female size, whereas male survival was independent of size. Overall, sex had a significant effect on survival independent of habitat type (monthly survival probability: 0.80 for males vs. up to 0.89 for females of optimal size). We discuss our results in relation to the potential mechanisms involved in the observed phenotypic differentiation of A. homolechis and other lizard species in areas transformed by urbanization. Full article

(This article belongs to the Special Issue Feature Papers in 'Conservation Biology and Biodiversity')

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19 pages, 4835 KiB

Open AccessArticle

Abundant Genetic Diversity Harbored by Traditional Naked Barley Varieties on Tibetan Plateau: Implications in Their Effective Conservation and Utilization

by NiMa QuZhen, Lhundrup Namgyal, Dawa Dondrup, Ying Wang, Zhi Wang, Xing-Xing Cai, Bao-Rong Lu and La Qiong

Biology 2024, 13(12), 1018; https://doi.org/10.3390/biology13121018 - 5 Dec 2024

Abstract

Naked barley (Hordeum vulgare var. nudum) is a staple food crop, contributing significantly to global food security. Understanding genetic diversity will facilitate its effective conservation and utilization. To determine genetic diversity and its distribution within and among varieties, we characterized 30 [...] Read more.

Naked barley (Hordeum vulgare var. nudum) is a staple food crop, contributing significantly to global food security. Understanding genetic diversity will facilitate its effective conservation and utilization. To determine genetic diversity and its distribution within and among varieties, we characterized 30 naked barley varieties from Tibet, representing the traditional, modern, and germplasm-resources-bank gene pools, by analyzing SSR molecular fingerprints. The results demonstrate abundant genetic diversity in Tibetan naked barley varieties, particularly those in the traditional gene pool that holds much more private (unique) alleles. Principal coordinates and STRUCTURE analyses indicate substantial deviation of the modern varieties from the traditional and germplasm-resources-bank varieties. A considerable amount of seed mixture is detected in the modern varieties, suggesting the practices of using mixed seeds in modern-variety cultivation. Cluster analyses further indicate the narrow genetic background of the modern varieties, likely due to the limited number of traditional/germplasm-resources-bank varieties applied in breeding. Relationships between increases in genetic diversity and sample sizes within naked barley varieties highlight the importance of effective sampling strategies for field collections. The findings from this study have important implications for the sustainable utilization and effective conservation of different types of naked barley germplasm, both in Tibet and in other regions around the world. Full article

(This article belongs to the Section Plant Science)

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19 pages, 5530 KiB

Open AccessReview

Pregametogenesis: The Earliest Stages of Gonad and Germline Differentiation in Anuran Amphibians

by Maria Ogielska, Magdalena Chmielewska and Beata Rozenblut-Kościsty

Biology 2024, 13(12), 1017; https://doi.org/10.3390/biology13121017 - 5 Dec 2024

Abstract

The gonads of amphibians, like other vertebrates, consist of somatic tissues, which create a specific environment essential for the differentiation of germline cells. The earliest stages of gametogenesis still remain underexplored in anuran amphibians. We propose to introduce the term “pregametogenesis” for a [...] Read more.

The gonads of amphibians, like other vertebrates, consist of somatic tissues, which create a specific environment essential for the differentiation of germline cells. The earliest stages of gametogenesis still remain underexplored in anuran amphibians. We propose to introduce the term “pregametogenesis” for a specific period of gonocyte proliferation and differentiation that occurs exclusively during the early stages of gonadal development. This review shows the key steps of early gonad differentiation in anuran amphibians and further compares chromatin reorganization in gonocytes of mammals and hybridogenetic water frogs. In mammals, this phase involves resetting genomic imprinting, which is crucial for determining gene expression in offspring. In hybridogenetic Pelophylax water frogs, we highlight the unique phenomenon of genome elimination, where one parental subgenome is eliminated while the other is replicated. This process, occurring at the same developmental phase as imprinting in mammals, underscores the evolutionary importance of pregametogenesis. The study of amphibian gonocytes provides valuable insights into chromatin reorganization and genome plasticity, offering new perspectives on reproductive biology. Full article

(This article belongs to the Special Issue Mechanisms of Sex Determination and Gonad Development)

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