Agronomy

20 pages, 2685 KiB

Open AccessArticle

Supplementary Light and Retardant Application During the Rooting Stage Improve the Final Performances of Petunia and Calibrachoa Plants

by Bożena Szewczyk-Taranek, Iwona Domagała-Świątkiewicz, Anna Kapczyńska, Paweł Marcinkowski and Bożena Pawłowska

Agronomy 2025, 15(7), 1644; https://doi.org/10.3390/agronomy15071644 (registering DOI) - 6 Jul 2025

Abstract

The efficient production of high-quality bedding plants is essential for greenhouse growers aiming to meet market demands while minimising costs. This study investigated the effects of supplemental lighting (prolonged photoperiod) and retardant treatments on the growth, development, mineral composition, and flowering of vegetatively [...] Read more.

The efficient production of high-quality bedding plants is essential for greenhouse growers aiming to meet market demands while minimising costs. This study investigated the effects of supplemental lighting (prolonged photoperiod) and retardant treatments on the growth, development, mineral composition, and flowering of vegetatively propagated Petunia × atkinsiana (Surfinia^® ‘Lime’ and ‘Purple’) and Calibrachoa × hybrida (Superbells^® ‘Unique Red’ and ‘Unique Golden Yellow’) cultivars. The plants were subjected to extended lighting for up to 16 h during the rooting phase and treated with or without a growth retardant. The supplemental light significantly accelerated the flowering, improved the shoot branching, and enhanced the mineral nutrient accumulation, particularly of calcium, sulphur, and magnesium. The illuminated plants also exhibited higher chlorophyll content and more favourable dry-mass accumulation. The growth retardants reduced the plant height and concentrations of nitrogen, phosphorus, and potassium but increased the calcium and magnesium contents. The combination of lighting and growth regulation optimised the plant morphology and nutritional status, producing compact, well-branched plants with enhanced visual appeal. Earlier flowering of illuminated plants is valuable from a commercial perspective, allowing earlier selling and improved product marketability. These findings confirm the synergistic benefits of early photoperiod control and chemical growth regulation in enhancing the commercial quality and sustainability of bedding plant production. Full article

(This article belongs to the Section Horticultural and Floricultural Crops)

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16 pages, 792 KiB

Open AccessArticle

Measuring the Sustainability of Nitrogen Fertilization in EU Agriculture: A New Index-Based Assessment in the Context of Sustainable Intensification

by Magdalena Szymańska, Piotr Sulewski, Adam Wąs and Tomasz Sosulski

Agronomy 2025, 15(7), 1643; https://doi.org/10.3390/agronomy15071643 (registering DOI) - 6 Jul 2025

Abstract

This study comprehensively evaluated nitrogen (N) management in 27 European countries from 1990 to 2021, utilizing the FAO and LUCAS databases. The EU countries were categorized into four groups based on their agricultural production intensities: low (L), medium–low (ML), medium–high (MH), and high [...] Read more.

This study comprehensively evaluated nitrogen (N) management in 27 European countries from 1990 to 2021, utilizing the FAO and LUCAS databases. The EU countries were categorized into four groups based on their agricultural production intensities: low (L), medium–low (ML), medium–high (MH), and high (H). Additionally, a new Sustainable Nitrogen Management Indicator (SNMI) has been introduced to measure the sustainability of agricultural production. The analyses reveal significant variation in nitrogen fertilization intensity among EU countries, which correlates with differences in yield levels. Generally, higher fertilization leads to higher nutrient loss; however, the nitrogen losses per unit of yield show only minor differences between high- and low-intensity countries. From 1990 to 2021, a general improvement was observed in nitrogen management across all groups, as evidenced by a significant decline in the SNMI, indicating that agricultural production has become more sustainable. Notably, low-intensity countries showed the most significant improvement, with increased nitrogen input per hectare since the 1990s, demonstrating that moderate fertilization can enhance N use efficiency. In contrast, high-intensity countries saw decreased nitrogen inputs but still improved SNMI. These trends support the idea of sustainable intensification. The multidimensional SNMI comprehensively assesses eco-efficiency by highlighting environmental threats and production benefits. This paper demonstrates that SNMI is robust and easy to calculate using available datasets, and it can be implemented to assess nitrogen management efficiency at various scales. Full article

(This article belongs to the Special Issue Safe and Efficient Utilization of Water and Fertilizer in Crops)

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29 pages, 8004 KiB

Open AccessArticle

The Development of an Air Suction Precision Seed-Metering Device for Rice Plot Breeding

by Wei Qin, Yuwu Li, Cheng Qian, Zhuorong Fan, Daoqing Yan, Guo Zou, Siqian Liu, Zaiman Wang, Ying Zang and Minghua Zhang

Agronomy 2025, 15(7), 1642; https://doi.org/10.3390/agronomy15071642 (registering DOI) - 5 Jul 2025

Abstract

To address the lack of specialized seeding equipment and low manual seeding efficiency in rice plot breeding, this study developed an air suction precision seed-metering device for rice plot breeding, featuring automatic seed-switching and seed-clearing functions controlled by an STM32 microcontroller. Firstly, based [...] Read more.

To address the lack of specialized seeding equipment and low manual seeding efficiency in rice plot breeding, this study developed an air suction precision seed-metering device for rice plot breeding, featuring automatic seed-switching and seed-clearing functions controlled by an STM32 microcontroller. Firstly, based on morphological analysis and MATLAB image processing, an active contour method was used to construct a suction hole model. Secondly, to meet the non-contaminated switching requirements between rice varieties, an electrically controlled seed-switching and seed-clearing mechanism was developed based on QR code-based precise recognition and positioning. Using 10 rice varieties as experimental materials, performance tests were conducted. The results showed that the seed-switching mechanism had single and cumulative errors under 0.4°, and the seed-clearing rate reached 100% with an average clearing time below 0.88 s. At a rotational speed of 20 r·min⁻¹ and negative pressure of 3200 Pa, seed-filling performance was optimal for all rice varieties. Among them, the rice variety Nayou 6388 exhibited the best seed-filling performance, with a 0.8% missing seed rate, 97.6% single and double seed rate, and 1.6% multiple seed rate. In double-row coordinated tests, each seed-metering device independently completed seed switching and maintained synchronized operation, meeting agronomic requirements for accurate seed switching/clearing and precision seed filling in rice plot breeding. Full article

(This article belongs to the Collection Advances of Agricultural Robotics in Sustainable Agriculture 4.0)

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23 pages, 4667 KiB

Open AccessArticle

An Experimental Study on the Charging Effects and Atomization Characteristics of a Two-Stage Induction-Type Electrostatic Spraying System for Aerial Plant Protection

by Yufei Li, Qingda Li, Jun Hu, Changxi Liu, Shengxue Zhao, Wei Zhang and Yafei Wang

Agronomy 2025, 15(7), 1641; https://doi.org/10.3390/agronomy15071641 (registering DOI) - 5 Jul 2025

Abstract

To address the technical problems of broad droplet size spectrum, insufficient atomization uniformity, and spray drift in plant protection unmanned aerial vehicle (UAV) applications, this study developed a novel two-stage aerial electrostatic spraying device based on the coupled mechanisms of hydraulic atomization and [...] Read more.

To address the technical problems of broad droplet size spectrum, insufficient atomization uniformity, and spray drift in plant protection unmanned aerial vehicle (UAV) applications, this study developed a novel two-stage aerial electrostatic spraying device based on the coupled mechanisms of hydraulic atomization and electrostatic induction, and, through the integration of three-dimensional numerical simulation and additive manufacturing technology, a new two-stage inductive charging device was designed on the basis of the traditional hydrodynamic nozzle structure, and a synergistic optimization study of the charging effect and atomization characteristics was carried out systematically. With the help of a charge ratio detection system and Malvern laser particle sizer, spray pressure (0.25–0.35 MPa), charging voltage (0–16 kV), and spray height (100–1000 mm) were selected as the key parameters, and the interaction mechanism of each parameter on the droplet charge ratio (C/m) and the particle size distribution (Dv50) was analyzed through the Box–Behnken response surface experimental design. The experimental data showed that when the charge voltage was increased to 12 kV, the droplet charge-to-mass ratio reached a peak value of 1.62 mC/kg (p < 0.01), which was 83.6% higher than that of the base condition; the concentration of the particle size distribution of the charged droplets was significantly improved; charged droplets exhibited a 23.6% reduction in Dv50 (p < 0.05) within the 0–200 mm core atomization zone below the nozzle, with the coefficient of variation of volume median diameter decreasing from 28.4% to 16.7%. This study confirms that the two-stage induction structure can effectively break through the charge saturation threshold of traditional electrostatic spraying, which provides a theoretical basis and technical support for the optimal design of electrostatic spraying systems for plant protection UAVs. This technology holds broad application prospects in agricultural settings such as orchards and farmlands. It can significantly enhance the targeted deposition efficiency of pesticides, reducing drift losses and chemical usage, thereby enabling agricultural enterprises to achieve practical economic benefits, including reduced operational costs, improved pest control efficacy, and minimized environmental pollution, while generating environmental benefits. Full article

(This article belongs to the Section Agricultural Biosystem and Biological Engineering)

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17 pages, 4162 KiB

Open AccessArticle

Silver Nanoparticles Embedded in Sodium Alginate: Antibacterial Efficacy and Effects on Red Cabbage Seedling Performance

by Miłosz Rutkowski, Wojciech Makowski, Lidia Krzemińska-Fiedorowicz, Karen Khachatryan, Andrzej Kalisz, Dagmara Malina, Jarosław Chwastowski, Zbigniew Wzorek, Gohar Khachatryan, Agnieszka Sękara and Anna Kołton

Agronomy 2025, 15(7), 1640; https://doi.org/10.3390/agronomy15071640 (registering DOI) - 5 Jul 2025

Abstract

Innovative plant protection solutions are increasingly sought in modern agriculture. Rapid advances in nanotechnology offer promising opportunities to develop biodegradable, cost-effective composites containing silver nanoparticles (AgNPs) with well-documented antimicrobial properties. The aim of this study was to synthesize sodium alginate gels containing AgNPs, [...] Read more.

Innovative plant protection solutions are increasingly sought in modern agriculture. Rapid advances in nanotechnology offer promising opportunities to develop biodegradable, cost-effective composites containing silver nanoparticles (AgNPs) with well-documented antimicrobial properties. The aim of this study was to synthesize sodium alginate gels containing AgNPs, evaluate their physicochemical and antibacterial properties, and assess their effect on the growth of red cabbage (Brassica oleracea var. capitata f. rubra) seedlings. In accordance with the principles of green chemistry, AgNPs were chemically synthesized using sodium alginate as a stabilizer and fructose as a non-toxic reducing agent. The final composite contained 150 mg/L AgNPs and was diluted to 20 and 60 mg/L for biological tests. Antibacterial activity against Bacillus cereus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa was tested using agar diffusion assays. Seedling growth parameters and phytochemical content were assessed after 10 days of seedlings exposure to AgNPs. The results showed significant antibacterial activity in all tested strains. Crucially, neither AgNPs concentration negatively affected seedling development or phytochemical concentration. Application of AgNPs at concentration of 60 mg/L increased ascorbic acid and carotenoids content in comparison to control (deionized water). These results suggest that AgNPs-alginate composites may serve as sustainable antimicrobial agents in agriculture, inhibiting pathogens without compromising crop quality. Full article

(This article belongs to the Special Issue Application of Nanomaterials for Diseases and Pest Control in Agriculture)

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10 pages, 3322 KiB

Open AccessArticle

Adequate Irrigation Amount per Application Is Required to Secure Uniform Water Management in Drip Irrigation Systems

by Sooeon Lee, Lynne Seymour and Jongyun Kim

Agronomy 2025, 15(7), 1639; https://doi.org/10.3390/agronomy15071639 (registering DOI) - 5 Jul 2025

Abstract

Soil moisture sensor-based drip irrigation enables efficient irrigation practices by delivering the required water to plants. However, efficiency must be accompanied by uniform water management and crop growth. This study examined the effect of different irrigation amounts (IAs) per application (5.5, 55, 110, [...] Read more.

Soil moisture sensor-based drip irrigation enables efficient irrigation practices by delivering the required water to plants. However, efficiency must be accompanied by uniform water management and crop growth. This study examined the effect of different irrigation amounts (IAs) per application (5.5, 55, 110, and 165 mL) on the uniformity of substrate volumetric water content (VWC) within an irrigation plot, and the corresponding effect on sweet basil growth uniformity. Sixty-four frequency domain reflectometry sensors monitored the VWC of each 440 mL pot, and drip irrigation was automatically applied at 0.3 m³·m⁻³. The 5.5 mL IA showed the highest water use efficiency; however, it also resulted in considerable non-uniform VWC (coefficient of variation, CV = 0.404). In contrast, the 110 and 165 mL IAs provided better VWC uniformity (CV = 0.073 and 0.075, respectively), suggesting that less frequent, but larger IAs improved VWC uniformity. Despite the differences in VWC uniformity among treatments, the growth and physiological responses were quite similar across the treatments. It was found that supplying 110 mL irrigation water via the soil moisture sensor-based drip irrigation system to sweet basil plants in 440 mL pots is optimal for achieving both water use efficiency and VWC uniformity. Full article

(This article belongs to the Section Water Use and Irrigation)

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15 pages, 1253 KiB

Open AccessArticle

Laboratory Optimization of Poultry Feather Hydrolysate Application for Soil Fertility: Effects of Soil Texture and Hydrolysate State and Addition Rate

by Aditi Roy and Veronika Jílková

Agronomy 2025, 15(7), 1638; https://doi.org/10.3390/agronomy15071638 (registering DOI) - 5 Jul 2025

Abstract

The application of soil organic amendments is a well-established approach to enhancing soil fertility; yet the effects of poultry feather hydrolysate (PFH) on temperate coarse-textured agricultural soils remain underexplored. A six-month microcosm experiment was conducted to determine the effects of PFH in different [...] Read more.

The application of soil organic amendments is a well-established approach to enhancing soil fertility; yet the effects of poultry feather hydrolysate (PFH) on temperate coarse-textured agricultural soils remain underexplored. A six-month microcosm experiment was conducted to determine the effects of PFH in different states (liquid or solid) and addition rates (none, low, or high; i.e., 0, 4, or 8 t dw ha⁻¹, respectively) on microbial activity, nutrient availability and retention, and organic matter (OM) stabilization in two coarse-textured soils (loamy sand or sandy loam). Sandy loam soil exhibited a stronger response to PFH application, supporting 20% higher microbial activity, 35% higher nutrient retention, and 89% higher OM content in soil aggregates compared to loamy sand soil, reflecting enhanced OM stabilization. Moreover, PFH in the liquid state demonstrated more prolonged microbial activity and more sustained release of nutrients compared to the solid state. Finally, at the end of incubation, the high addition rate of PFH significantly increased soil nutrient content by 106%, while the low addition rate limited the increase to 39%, both compared to the no addition rate. Overall, the results suggest that PFH, particularly in the liquid state and at the high addition rate, serves as an effective soil organic amendment, enhancing microbial activity and soil fertility while emphasizing the importance of soil texture in optimizing its application. Full article

(This article belongs to the Section Farming Sustainability)

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22 pages, 1962 KiB

Open AccessArticle

Effects of Low-Temperature Plasma Treatment on Germination, Seedling Development, and Biochemical Parameters of Long-Term-Stored Seeds

by Martin Matějovič, Vladislav Čurn, Jan Kubeš, Eva Jozová, Zora Kotíková and Petra Hlásná Čepková

Agronomy 2025, 15(7), 1637; https://doi.org/10.3390/agronomy15071637 (registering DOI) - 4 Jul 2025

Abstract

The promising field of low-temperature plasma treatment, known for its non-invasive and environmentally sustainable nature, is being actively investigated for its ability to enhance germination, emergence, yield, and overall plant development in a broad spectrum of crops. For gene bank requirements, low-temperature plasma [...] Read more.

The promising field of low-temperature plasma treatment, known for its non-invasive and environmentally sustainable nature, is being actively investigated for its ability to enhance germination, emergence, yield, and overall plant development in a broad spectrum of crops. For gene bank requirements, low-temperature plasma technologies can also improve germination parameters and promote the development seeds suitable for long-term storage. Seeds from four selected cultivars of wheat, oats, flax, and rapeseed stored in the gene bank for 1, 10, and 20 years were subjected to plasma treatments for 20, 25, and 30 min. The study evaluated the mean root and shoot length, root–shoot ratio, and seedling vigour index. Additionally, the malondialdehyde level, total polyphenol content, total flavonoid content, and total antioxidant capacity were analysed. Plasma treatment displayed varying effects on the morphological characteristics and antioxidant activity of the tested cultivars, which were influenced by treatment duration and cultivar. A positive effect of plasma treatment on seedling length, seedling vigour index, and root–shoot ratio was observed in flax cultivar ‘N-9/62/K3/B’ in all periods and in variants T2 and T3. Conversely, the wheat cultivar ‘Granny’ showed variable results, and the oat cultivar ‘Risto’ showed variable negative results in regards to mean root length and mean shoot length after plasma treatment. The indicators of oxidative stress and antioxidant capacity were affected in all the cultivars studied. A positive effect of plasma treatment on these indicators was observed in the wheat cultivar ‘Granny’, while flax cultivar ‘N-9/62/K3/B’ exhibited inconsistent results. While in cereals, a decrease in malondialdehyde content after plasma treatment was associated with an increase in polyphenol and flavonoid content as the treatment duration increased, small-seeded species responded somewhat differently. The rapeseed cultivar ‘Skrivenskij’ and flax cultivar ‘N-9/62/K3/B’ showed an increase in polyphenol and flavonoid content following a decrease in malondialdehyde levels. This study highlights the potential of low-temperature plasma treatment for long-term-stored seeds and its applicability to plant genetic resources. The findings emphasize the need for the further optimization of low-temperature plasma treatment conditions for different plant species and cultivars. Full article

(This article belongs to the Section Farming Sustainability)

19 pages, 5884 KiB

Open AccessArticle

Partitioned Recirculating Renovation for Traditional Rice–Fish Farming Induced Substantial Alterations in Bacterial Communities Within Paddy Soil

by Yiran Hou, Hongwei Li, Rui Jia, Linjun Zhou, Bing Li and Jian Zhu

Agronomy 2025, 15(7), 1636; https://doi.org/10.3390/agronomy15071636 - 4 Jul 2025

Abstract

Integrated agriculture–aquaculture (IAA), represented by integrated rice–fish farming, offers a sustainable production method that addresses global food issues and ensures food security. Partitioned recirculating renovation based on traditional integrated rice–fish farming is an effective way to facilitate the convenient harvesting of aquatic products [...] Read more.

Integrated agriculture–aquaculture (IAA), represented by integrated rice–fish farming, offers a sustainable production method that addresses global food issues and ensures food security. Partitioned recirculating renovation based on traditional integrated rice–fish farming is an effective way to facilitate the convenient harvesting of aquatic products and avoid difficulties associated with mechanical operations. To elucidate the impact of partitioned recirculating renovation on the bacterial communities within paddy field ecosystems, we investigated the soil environmental conditions and soil bacterial communities within integrated rice–fish farming, comparing those with and without partitioned recirculating renovations. The findings indicated a significant reduction in the bacterial community richness within paddy soil in the ditch (fish farming area), along with noticeable changes in the relative proportions of the predominant bacterial phyla in both the ditch and the rice cultivation area following the implementation of partitioned recirculating renovation. In both the ditch and the rice cultivation area, partitioned recirculating renovation diminished the edges and nodes in the co-occurrence networks for soil bacterial communities and considerably lowered the robustness index, negatively impacting the stability of bacterial communities in paddy soil. Simultaneously, the partitioned recirculating renovation substantially influenced the bacterial community assembly process, enhancing the relative contributions of stochastic processes such as dispersal limitation, drift, and homogenizing dispersal. In addition, partitioned recirculating renovation significantly altered the soil environmental conditions in both the ditch and the rice cultivation area, with environmental factors being markedly correlated with the soil bacterial community, especially the total nitrogen (TN) and total phosphorus (TP), which emerged as the primary environmental drivers influencing the soil bacterial community. Overall, these results elucidated the ecological impacts of partitioned recirculating renovation on the paddy soil from a microbiomic perspective, providing a microbial basis for optimizing partitioned rice–fish systems. Full article

(This article belongs to the Special Issue Microbial Interactions and Functions in Agricultural Ecosystems)

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11 pages, 2183 KiB

Open AccessArticle

Effects of Light Supplementation on Lettuce Growth, Yield, and Water Use During Winter Season in North Mississippi

by Ibukun T. Ayankojo, Thomas Horgan and Jeff Wilson

Agronomy 2025, 15(7), 1635; https://doi.org/10.3390/agronomy15071635 - 4 Jul 2025

Abstract

Most vegetable crop production in Mississippi (MS) occurs during the summer, characterized by high temperature and relative humidity. Lettuce yield and harvest quality are significantly affected by heat stress. To avoid the heat stress of the summer months, lettuce production in MS is [...] Read more.

Most vegetable crop production in Mississippi (MS) occurs during the summer, characterized by high temperature and relative humidity. Lettuce yield and harvest quality are significantly affected by heat stress. To avoid the heat stress of the summer months, lettuce production in MS is either produced in controlled environments or during the winter months with cooler temperatures. This period, however, coincides with months with low solar radiation and shorter day length, resulting in a longer growing season and poor harvest quality. Therefore, this study was conducted to determine the optimum duration of light supplement on the growth, yield, and water use of greenhouse (GH) lettuce during the winter season in north Mississippi. In this study, three daily supplemental light duration regimes, 0 h, 4 h, and 8 h, starting at sunset, were evaluated across two lettuce cultivars, Green Forest (GF) and Ruby (RB). The study indicated that supplemental lighting significantly increased lettuce growth, yield, and water use. Although day length extension from 4 to 8 h of supplemental light had no yield benefits on the RB cultivar, extending day length from 4 to 8 h increased GF yield by 42%. It was also observed that the effects of light supplementation during low natural light quality at early or later growth stages differ between cultivars. Based on the results obtained from this study, a 4 h and 8 h post-sunset light supplementation is considered optimum for RB and GF lettuce cultivars, respectively, during the winter growing season in MS. Full article

(This article belongs to the Section Horticultural and Floricultural Crops)

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16 pages, 2833 KiB

Open AccessArticle

Design and Tests of a Large-Opening Flexible Seedling Pick-Up Gripper with Multiple Grasping Pins

by Luhua Han, Meijia Zhang, Yan Wang, Guoxin Ma, Qizhi Yang and Yang Liu

Agronomy 2025, 15(7), 1634; https://doi.org/10.3390/agronomy15071634 - 4 Jul 2025

Abstract

The pick-up gripper, as a core component of automatic transplanting systems, presents challenges in reliably grasping seedlings. In this study, a large-opening flexible seedling pick-up gripper was designed based on standard trays and seedling characteristics. Structural design and force analysis of the grasping [...] Read more.

The pick-up gripper, as a core component of automatic transplanting systems, presents challenges in reliably grasping seedlings. In this study, a large-opening flexible seedling pick-up gripper was designed based on standard trays and seedling characteristics. Structural design and force analysis of the grasping mechanism were conducted to develop a functional prototype. As this represented the first prototype of this new gripper, multi-factor orthogonal tests and performance tests under local conditions were performed to evaluate its grasping effectiveness. It was found that the end diameter of the pick-up pin and the extraction speed for lifting plug seedlings vertically had the most significant effects, followed by the penetration depth and grasping force. The optimum grasping effectiveness was achieved when the end diameter of the pick-up pin was 1.2 mm, the penetration depth in the top straight line of the pick-up pin was 40 mm, the grasping force for squeezing root lumps was 0.4 MPa, and the extraction speed for lifting plug seedlings in a vertical direction was 900 mm/s. For typical vegetable seedlings, the average success rate in transplanting was up to 95%. Under the combined actions of penetrating, squeezing, and extracting operations, plug seedlings could be efficiently picked out for efficient transplanting. Full article

(This article belongs to the Special Issue Precision Operation Technology and Intelligent Equipment in Farmland—3rd Edition)

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20 pages, 3098 KiB

Open AccessArticle

Exploring Coffee Silverskin as a Sustainable Peat Additive in the Plant Nursery Production

by Natalia Miler, Piotr Wojewódzki, Anita Woźny, Dominika Rymarz and Agnieszka Gołębiewska

Agronomy 2025, 15(7), 1633; https://doi.org/10.3390/agronomy15071633 - 4 Jul 2025

Abstract

Sustainable alternatives to peat in horticultural substrates are increasingly sought. This study assessed the use of coffee silverskin (CS), a byproduct of coffee roasting, as a substrate component for rooting and growing ornamental plants—Buddleja, Lythrum, and Veronica. Plants were [...] Read more.

Sustainable alternatives to peat in horticultural substrates are increasingly sought. This study assessed the use of coffee silverskin (CS), a byproduct of coffee roasting, as a substrate component for rooting and growing ornamental plants—Buddleja, Lythrum, and Veronica. Plants were cultivated in peat-based substrates with 0%, 25%, 50%, and 75% CS addition. In order to determine the effect of substrate modification with CS, the following parameters were analyzed: rooting efficiency, plant growth, pigment content, physiological indices (SPAD, Fv/Fm, NFI), and substrate properties. A 25% CS addition supported high rooting success (94.4% on average) without negatively affecting root or shoot traits, and even improved flowering earliness. At 50% CS, Buddleja showed moderate tolerance, while Lythrum and Veronica performed poorly. The substrate with 75% CS addition significantly reduced rooting and growth across all species. Elevated pH and electrical conductivity in high-CS substrates likely contributed to plant stress. Physiological indicators confirmed minimal stress at 25% CS, but increased stress response at 75%. Overall, CS can replace up to 25% of peat in substrates without compromising plant performance, offering a sustainable alternative in nursery production. However, higher CS levels require structural or chemical adjustments to reduce compaction and stress effects. Further research is needed to improve CS-based substrate formulations for broader horticultural use. Full article

(This article belongs to the Section Horticultural and Floricultural Crops)

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12 pages, 2664 KiB

Open AccessArticle

Heavy Metal Immobilization by Phosphate-Solubilizing Fungus and Phosphogypsum Under the Co-Existence of Pb(II) and Cd(II)

by Xu Li, Zhenyu Chao, Haoxuan Li, Jiakai Ji, Xin Sun, Yingxi Chen, Zhengda Li, Zhen Li, Chuanhao Li, Jun Yao and Lan Xiang

Agronomy 2025, 15(7), 1632; https://doi.org/10.3390/agronomy15071632 - 4 Jul 2025

Abstract

Globally, phosphogypsum (PG) is the primary by-product of the phosphorus industry. Aspergillus niger (A. niger), one of the most powerful types of phosphate-solubilizing fungi (PSF), can secrete organic acids to dissolve the phosphates in PG. This study investigated heavy metal (HM) [...] Read more.

Globally, phosphogypsum (PG) is the primary by-product of the phosphorus industry. Aspergillus niger (A. niger), one of the most powerful types of phosphate-solubilizing fungi (PSF), can secrete organic acids to dissolve the phosphates in PG. This study investigated heavy metal (HM) remediation by PG and A. niger under the co-existence of Pb and Cd. It demonstrated that 1 mmol/L Pb²⁺ stimulated the bioactivity of A. niger during incubation, based on the CO₂ emission rate. PG successfully functioned as P source for the fungus, and promoted the growth of the fungal cells. Meanwhile, it also provided sulfates to immobilize Pb in the solution. The subsequently generated anglesite was confirmed using SEM imaging. The immobilization rate of Pb reached over 95%. Under co-existence, Pb²⁺ and 0.01 mmol/L Cd²⁺ maximized the stimulating effect of A. niger. However, the biotoxicity of Pb²⁺ and elevated Cd²⁺ (0.1 mmol/L) counterbalanced the stimulating effect. Finally, 1 mmol/L Cd²⁺ dramatically reduced the fungal activity. In addition, organic matters from the debris of A. niger could still bind Pb²⁺ and Cd²⁺ according to the significantly lowered water-soluble Pb and Cd concentrations. In all treatments with the addition of Cd²⁺, the relatively high biotoxicity of Cd²⁺ induced A. niger to absorb more Pb²⁺ to minimize the sorption of Cd²⁺ based on the XRD results. The functional group analysis of ATR-IR also confirmed the phenomenon. This pathway maintained the stability of Pb²⁺ immobilization using the fungus and PG. This study, hence, shed light on the application of A. niger and solid waste PG to remediate the pollution of Pb and Cd. Full article

(This article belongs to the Special Issue Environmental Ecological Remediation and Farming Sustainability—3rd Edition)

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20 pages, 3626 KiB

Open AccessArticle

Environmental, Genetic and Structural Interactions Affecting Phytophthora spp. in Citrus: Mixed Modelling and Mediation Analysis

by Dalal Boudoudou, Majid Mounir, Mohamed El bakkali, Allal Douira and Hamid Benyahia

Agronomy 2025, 15(7), 1631; https://doi.org/10.3390/agronomy15071631 - 4 Jul 2025

Abstract

This study investigates the complex interactions between environmental, genetic, and structural factors that influence two key parameters: the density of Phytophthora spp. propagules per gram of dry soil (NPSS) and the number of colonies (NC). Using advanced statistical approaches, we examined the combined [...] Read more.

This study investigates the complex interactions between environmental, genetic, and structural factors that influence two key parameters: the density of Phytophthora spp. propagules per gram of dry soil (NPSS) and the number of colonies (NC). Using advanced statistical approaches, we examined the combined effects of variables such as soil moisture, dry weight, temporal fluctuations, and rootstocks. The results show a significant linear relationship between NPSS and soil moisture, as well as a strong positive correlation between NPSS and NC. Genetic analyses reveal a predominant contribution of environmental factors to trait variability, with high phenotypic coefficient of variation (PCV) and low broad-sense heritability. Mixed models highlight the synergistic impact of soil moisture, NC, and dry soil weight on NPSS, as well as significant temporal effects. Mediation analysis confirms that soil moisture influences NPSS primarily through an indirect effect transmitted by NC, with a mediated proportion exceeding 94%. Finally, multivariate analysis reveals significant differences between rootstocks, with Citrus Volkameriana B2 28613 (R4) and Mandarin Sunki x P.T. B2 38581 (R7) standing out as the most performant. These results highlight the importance of an integrated management of environmental variables and rootstocks to optimize soil productivity and agronomic quality. The implications of this study provide a solid foundation for guiding genetic improvement and soil management strategies, balancing environmental constraints and the opportunities offered by targeted genetic selection. Full article

(This article belongs to the Section Pest and Disease Management)

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13 pages, 4028 KiB

Open AccessArticle

Influence of Dynamic Magnetic Field Exposure Duration on the Germination and Growth of Khao Dawk Mali 105 Rice Seed

by Tiwanat Gaewsondee, Cherdpong Chiawchanwattana, Phirayot Khaengkan, Juckamas Laohavanich, Chanat Vipattanaporn and Suphan Yangyuen

Agronomy 2025, 15(7), 1630; https://doi.org/10.3390/agronomy15071630 - 4 Jul 2025

Abstract

Magnetic field (MF) priming provides a chemical-free alternative to conventional methods; however, static exposure approaches are often limited by spatial heterogeneity in field–seed interaction caused by fixed seed positioning, undermining both treatment uniformity and reproducibility. To address this, the present study investigated the [...] Read more.

Magnetic field (MF) priming provides a chemical-free alternative to conventional methods; however, static exposure approaches are often limited by spatial heterogeneity in field–seed interaction caused by fixed seed positioning, undermining both treatment uniformity and reproducibility. To address this, the present study investigated the effects of dynamic MF exposure on the germination and early growth of Khao Dawk Mali 105 (KDML 105) rice seeds. A novel MF testing apparatus was developed using a 150 mT permanent magnet and a vortex-based air injection system designed to continuously rotate and redistribute seeds, ensuring uniform exposure. Seeds were treated for 0, 5, 10, 15, and 20 min to evaluate effects on vigor, germination, and seedling growth. The results showed that 5 and 10 min exposures significantly enhanced seed vigor (93.00% and 94.67%, respectively) compared to the control (83.33%), with 10 min yielding the highest improvement (p < 0.05, DMRT). Shoot and root growth also increased by 14.21% and 99.59%, respectively. These findings suggest that moderate-duration dynamic MF exposure is an efficient, eco-friendly priming technique for improving seed vigor and early growth. Future research should explore long-term agronomic impacts, economic feasibility, and varietal responses. The apparatus’s scalable design supports integration into industrial seed processing lines, advancing sustainable rice production. Full article

(This article belongs to the Section Agricultural Biosystem and Biological Engineering)

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15 pages, 2091 KiB

Open AccessReview

AI Roles in 4R Crop Pest Management—A Review

by Hengyuan Yang, Yuexia Jin, Lili Jiang, Jia Lu and Guoqi Wen

Agronomy 2025, 15(7), 1629; https://doi.org/10.3390/agronomy15071629 - 3 Jul 2025

Abstract

Insect pests are a major threat to agricultural production, causing significant crop yield reductions annually. Integrated pest management (IPM) is well-studied, but its precise application in farmlands is still challenging due to variable weather, diverse insect behaviors, crop variability, and soil heterogeneity. Recent [...] Read more.

Insect pests are a major threat to agricultural production, causing significant crop yield reductions annually. Integrated pest management (IPM) is well-studied, but its precise application in farmlands is still challenging due to variable weather, diverse insect behaviors, crop variability, and soil heterogeneity. Recent advancements in Artificial Intelligence (AI) have shown the potential to revolutionize pest management by implementing 4R pest stewardship: right pest identification, right method selection, right control timing, and right action taken. This review explores the roles of AI technologies within the 4R framework, highlighting AI models for accurate pest identification, computer vision systems for real-time monitoring, predictive analytics for optimizing control timing, and tools for selecting and applying pest control measures. Innovations in remote sensing, UAV surveillance, and IoT-enabled smart traps further strengthen pest monitoring and intervention strategies. By integrating AI into 4R pest management, this study underscores the potential of precision agriculture to develop sustainable, adaptive, and highly efficient pest control systems. Despite these advancements, challenges persist in data availability, model generalization, and economic feasibility for widespread adoption. The lack of interpretability in AI models also makes some agronomists hesitant to adopt these technologies. Future research should focus on scalable AI solutions, interdisciplinary collaborations, and real-world validation to enhance AI-driven pest management in field crops. Full article

(This article belongs to the Special Issue Intelligent Information System for Agriculture Based on Vision Technology)

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22 pages, 4025 KiB

Open AccessArticle

Effects of Different Land Use Types on Soil Quality and Microbial Diversity in Paddy Soil

by Ximei Zhao, Fengyun Xiang, Xicheng Wang, Mengchen Yang and Jifu Li

Agronomy 2025, 15(7), 1628; https://doi.org/10.3390/agronomy15071628 - 3 Jul 2025

Abstract

This study investigated the effects of three land use patterns—rice (Oryza sativa L.)–rapeseed (Brassica napus L.) rotation (Rapeseed), rice–shrimp (Procambarus clarkii G.) rotation (Shrimp), and the conversion of paddy fields to forestland (Forestland)—on aggregate structure, nutrient content, and microbial diversity in [...] Read more.

This study investigated the effects of three land use patterns—rice (Oryza sativa L.)–rapeseed (Brassica napus L.) rotation (Rapeseed), rice–shrimp (Procambarus clarkii G.) rotation (Shrimp), and the conversion of paddy fields to forestland (Forestland)—on aggregate structure, nutrient content, and microbial diversity in rice soils in Chuandian Town, Jingzhou District, Jianghan Plain, central China. The results revealed that the Shrimp treatment significantly increased soil organic matter (SOM), available nitrogen (AN), and available phosphorus (AP) content in the surface soil (0–10 cm) while reducing soil bulk density and improving pore structure. Forestland exhibited higher aggregate stability in deeper soil layers (20–40 cm), particularly in the 0.053–0.25 mm size fraction. Microbial diversity analysis showed that bacterial richness (Chao1 index) and diversity (Shannon index) were significantly higher in the Shrimp and Rapeseed treatments compared to those in the Forestland treatment, with Proteobacteria and Chloroflexi being the dominant bacterial phyla. Fungal communities were dominated by Ascomycota, withfForestland showing greater fungal richness in deeper soil. Soil depth significantly influenced aggregates, nutrients, and microbial diversity, with surface soil exhibiting higher values for these parameters than deeper layers. Redundancy analysis indicated that SOM, AP, and pH were the key drivers of bacterial community variation, while fungal communities were more influenced by nitrogen and porosity. Path analysis further demonstrated that land use patterns indirectly affected microbial diversity via altering aggregate structure and nutrient availability. Overall, the Shrimp treatment outperformed others in improving soil structure and nutrient supply, whereas the Forestland treatment was more conducive to promoting aggregate stability in deeper soil. Land use patterns indirectly regulated microbial communities through modifying soil aggregate structure and nutrient status, thereby influencing soil ecosystem health and stability. This study provides a theoretical basis for the sustainable management of rice soils, suggesting the optimization of rotation patterns in agricultural production to synergistically enhance soil physical, chemical, and biological properties. Full article

(This article belongs to the Section Agroecology Innovation: Achieving System Resilience)

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22 pages, 2196 KiB

Open AccessReview

A Review of IoT and Machine Learning for Environmental Optimization in Aeroponics

by Muhammad Amjad, Elanchezhian Arulmozhi, Yeong-Hyeon Shin, Moon-Kyung Kang and Woo-Jae Cho

Agronomy 2025, 15(7), 1627; https://doi.org/10.3390/agronomy15071627 - 3 Jul 2025

Abstract

Traditional farming practices are becoming increasingly inadequate to meet global food demand due to water scarcity, prolonged production cycles, climate variability, and declining arable land. In contrast, aeroponic, smart, soil-free farming technologies offer a more sustainable alternative by reducing land use and providing [...] Read more.

Traditional farming practices are becoming increasingly inadequate to meet global food demand due to water scarcity, prolonged production cycles, climate variability, and declining arable land. In contrast, aeroponic, smart, soil-free farming technologies offer a more sustainable alternative by reducing land use and providing efficient water use, given that aeroponics intermittently delivers water in mist form rather than maintaining continuous root zone moisture. However, aeroponics faces critical challenges in irrigation management due to non-standardized structures and limited real-time control. A key limitation is the inability to dynamically respond to temperature (T), relative humidity (RH), light intensity (L_i), electrical conductivity (EC), pH, and photosynthesis rate (P_n), resulting in suboptimal crop yields and resource wastage. Despite growing interest, there remains a research gap in integrating internet of things (IoT) and machine learning technologies into aeroponic systems for adaptive control. IoT-enabled sensors provide real-time data on ambient conditions and plant health, while ML models can adaptively optimize misting intervals based on the fluctuations in P_n and environmental inputs. These technologies are particularly well suited to address the dynamic, data-intensive nature of aeroponic environments. This review purposes a novel, standardized IoT–ML framework to control irrigation by emphasizing IoT sensing and ML-based decision making in aeroponics. This integrated approach is essential for minimizing water loss, enhancing resource efficiency, and advancing the sustainability of controlled-environment agriculture. Full article

(This article belongs to the Section Water Use and Irrigation)

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13 pages, 517 KiB

Open AccessArticle

Varied Susceptibility of Five Echinochloa Species to Herbicides and Molecular Identification of Species Using CDDP Markers

by Xiaoyan Wang, Lulu Ye, Jingui Zhou and Jun Li

Agronomy 2025, 15(7), 1626; https://doi.org/10.3390/agronomy15071626 - 3 Jul 2025

Abstract

Echinochloa spp. are among the most problematic malignant weeds in paddy fields. Under long-term herbicide selection pressure, they have developed resistances to multiple herbicides, leading to diminished control efficacy. Precision herbicide application, tailored to the susceptibility disparities among Echinochloa species, has emerged as [...] Read more.

Echinochloa spp. are among the most problematic malignant weeds in paddy fields. Under long-term herbicide selection pressure, they have developed resistances to multiple herbicides, leading to diminished control efficacy. Precision herbicide application, tailored to the susceptibility disparities among Echinochloa species, has emerged as a promising strategy to enhance weed control efficacy and decelerate herbicide resistance development. Nevertheless, the herbicide susceptibility variation across different Echinochloa taxa remain uncharted. Therefore, in this study, we determined the susceptibility of five Echinochloa species to 15 commonly used herbicides using the whole-plant bioassay method. Additionally, we explored the feasibility of employing the CDDP molecular marker technique for the rapid identification of distinct Echinochloa species. The results showing that five Echinochloa species exhibited differential susceptibility to 12 of the 15 herbicides tested underscore the necessity of personalized herbicide application strategies. Among the seven CDDP markers, KNOX-3 generated a specific band in the Echinochloa caudata population, which can be used to distinguish it from the other four Echinochloa species. The findings of this study will facilitate the precision application of herbicides for Echinochloa management in paddy fields. Full article

(This article belongs to the Special Issue Herbicides Toxicology and Weeds Herbicide-Resistant Mechanism—Series II)

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