The electrification of bus-based public transportation contributes to the goal of reducing the adverse environmental impacts caused by urban transportation. However, the penetration of electric vehicles has been slow due to their lower vehicle range and total costs in comparison to vehicles driven by internal combustion engines. By improving the powertrain efficiency, the total costs can be reduced for the same vehicle range. Therefore, this paper proposes a holistic design exploration approach to investigate and identify the optimal powertrain concept for electric city buses based on the component costs and energy consumption costs. The load profiles of speed, slope, and passenger occupancy profiles are derived for a selected bus route in Singapore, which is used in a powertrain design exploration for a 30-passenger vehicle. Six different powertrain architectures are analyzed, together with single and multi-speed gearbox configurations, to identify the optimal powertrain architecture and the resulting component sizes. The powertrain configurations are further analyzed in terms of their influence on the vehicle characteristics and total costs. Multi-motor configurations were found to have better vehicle characteristics and lower total costs in comparison to single rear motor configurations. Concepts with motors on the front and a rear axle could shift the load points to a higher efficiency region, resulting in lower energy consumption and energy costs. The optimal powertrain concept was a fixed-speed two-motor configuration, with a booster motor on the front axle and a motor on the rear axle. Full article

The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in nearly 20% of men’s fine perfumery. The biotechnological production of such fragrant sesquiterpenes is a promising alternative towards sustainability; nevertheless, product recovery from fermentation is one of the main constraints. In an effort to improve the (+)-zizaene recovery from a metabolically-engineered Escherichia coli, we developed an integrated bioprocess by coupling fermentation and (+)-zizaene recovery using adsorber extractants. Initially, (+)-zizaene volatilization was confirmed from cultivations with no extractants but application of liquid–liquid phase partitioning cultivation (LLPPC) improved (+)-zizaene recovery nearly 4-fold. Furthermore, solid–liquid phase partitioning cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion HP20 reached the highest recovery. Bioprocess was scaled up to 2 L bioreactors and in situ recovery configurations integrated to fermentation were evaluated. External recovery configuration was performed with an expanded bed adsorption column and improved (+)-zizaene titers 2.5-fold higher than LLPPC. Moreover, internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 2.2-fold, whereas adsorption velocity was determined as critical parameter for recovery efficiency. Consequently, IRC improved the (+)-zizaene titer 8.4-fold and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 211.13 mg L⁻¹ and productivity of 3.2 mg L⁻¹ h⁻¹. This study provides further knowledge for integration of terpene bioprocesses by in situ product recovery, which could be applied for many terpene studies towards the industrialization of fragrant molecules. Full article

Obesity is a major risk factor for obstructive sleep apnoea (OSA), the most common sleep-disordered breathing related to neurocognitive and metabolic syndromes, type II diabetes, and cardiovascular diseases. Although strongly recommended for this condition, there are no studies on the effectiveness of an interdisciplinary weight loss and lifestyle intervention including nutrition, exercise, sleep hygiene, and smoking and alcohol cessation. INTERAPNEA is a randomised controlled trial with a two-arm parallel design aimed at determining the effects of an interdisciplinary tailored weight loss and lifestyle intervention on OSA outcomes. The study will include 84 males aged 18–65 with a body mass index of ≥25 kg/m² and severe to moderate OSA randomly assigned to usual care (i.e., continuous positive airway pressure), or interdisciplinary weight loss and lifestyle intervention combined with usual care. Outcomes will be measured at baseline, intervention end-point, and six-month post-intervention, including apnoea-hypopnoea index (primary outcome), other neurophysical and cardiorespiratory polysomnographic outcomes, sleep quality, daily functioning and mood, body weight and composition, physical fitness, blood biomarkers, health-related quality of life, and cost-effectiveness. INTERAPNEA may serve to establish a cost-effective treatment not only for the improvement of OSA and its vast and severe comorbidities, but also for a potential remission of this condition. Full article

The concept of universality is a cornerstone of theories of critical phenomena. It is very well understood in most systems, especially in the thermodynamic limit. Finite-size systems present additional challenges. Even in low dimensions, universality of the edge and corner contributions to free energies and response functions is less investigated and less well understood. In particular, the question arises of how universality is maintained in correction-to-scaling in systems of the same universality class but with very different corner geometries. Two-dimensional geometries deliver the simplest such examples that can be constructed with and without corners. To investigate how the presence and absence of corners manifest universality, we analyze the spanning tree generating function on two different finite systems, namely the cobweb and fan networks. The corner free energies of these configurations have stimulated significant interest precisely because of expectations regarding their universal properties and we address how this can be delivered given that the finite-size cobweb has no corners while the fan has four. To answer, we appeal to the Ivashkevich–Izmailian–Hu approach which unifies the generating functions of distinct networks in terms of a single partition function with twisted boundary conditions. This unified approach shows that the contributions to the individual corner free energies of the fan network sum to zero so that it precisely matches that of the web. It therefore also matches conformal theory (in which the central charge is found to be $c=-2$ ) and finite-size scaling predictions. Correspondence in each case with results established by alternative means for both networks verifies the soundness of the Ivashkevich–Izmailian–Hu algorithm. Its broad range of usefulness is demonstrated by its application to hitherto unsolved problems—namely the exact asymptotic expansions of the logarithms of the generating functions and the conformal partition functions for fan and cobweb geometries. We also investigate strip geometries, again confirming the predictions of conformal field theory. Thus, the resolution of a universality puzzle demonstrates the power of the algorithm and opens up new applications in the future. Full article

In cells the interorganelle communication comprises vesicular and non-vesicular mechanisms. Non-vesicular material transfer predominantly takes place at regions of close organelle apposition termed membrane contact sites and is facilitated by a growing number of specialized proteins. Contacts of the endoplasmic reticulum (ER) and mitochondria are now recognized to be essential for diverse biological processes such as calcium homeostasis, phospholipid biosynthesis, apoptosis, and autophagy. In addition to these universal roles, ER-mitochondria communication serves also cell type-specific functions. In this review, we summarize the current knowledge on ER-mitochondria contacts in cells of the innate immune system, especially in macrophages. We discuss ER- mitochondria communication in the context of macrophage fatty acid metabolism linked to inflammatory and ER stress responses, its roles in apoptotic cell engulfment, activation of the inflammasome, and antiviral defense. Full article

Marine polar lipids (PLs) have exhibited promising cardioprotection. In this study, marine by-products such as salmon heads (SHs), their brain, eyes and main optic nerves (SBEON), and head-remnants after SBEON removal (RemSH), as well as herring fillets (HFs), herring heads (HHs) and minced boarfish (MB), were evaluated as potential sustainable sources of such bioactive PLs. The antithrombotic bioactivities of PLs derived from these marine by-products were assessed for the first time in human platelets against platelet-activating factor (PAF), thrombin, collagen, and adenosine diphosphate (ADP), while their fatty acid composition was evaluated by gas chromatography–mass spectrometry (GC-MS). PLs from all marine by-products tested possess strong antithrombotic activities against aggregation of human platelets induced by all platelet agonists tested. RemSH, SBEON, HHs, HFs, and MB exhibited strong anti-PAF effects, similar to those previously reported for salmon fillets. PLs from MB had the strongest anti-collagen effects and PLs from SHs and SBEON were the most active against thrombin and ADP. PLs from HHs had similar antithrombotic effects with those from HFs in all agonists. RemSH was less active in all agonists, suggesting that SBEON is the main source of bioactive PLs in SHs. All PLs were rich in omega-3 polyunsaturated fatty acids (ω3PUFA), such as docosahexaenoic acid (DHA) and eicosapentaenoic (EPA) acid, with favourable low values of the ω6/ω3 ratio. Salmon, herring, and boarfish by-products are rich sources of bioactive marine PLs with potent antithrombotic and cardioprotective properties. Full article

Taxi demand prediction is one of the key factors in making online taxi hailing services more successful and more popular. Accurate taxi demand prediction can bring various advantages including, but not limited to, enhancing user experience, increasing taxi utilization, and optimizing traffic efficiency. However, the task is challenging because of complex spatial and temporal dependencies of taxi demand. In addition, relationships between non-adjacent regions are also critical for accurate taxi demand prediction, whereas they are largely ignored by existing approaches. To this end, we propose a novel graph and time-series learning model for city-wide taxi demand prediction in this paper. It has two main building blocks, the first one utilize a graph network with attention mechanism to effectively learn spatial dependencies of taxi demand in a broader perspective of the entire city, and the output at each time interval is then transferred to the second block. In the graph network, the edge is defined by an Origin–Destination relation to capture non-adjacent impacts. The second one uses a neural network which is adept with processing sequence data to capture the temporal correlations of city-wide taxi demand. Using a large, real-world dataset and three metrics, we conduct an extensive experimental study and find that our model outperforms state-of-the-art baselines by 9.3% in terms of the root-mean-square error. Full article

The purpose of this article is to introduce a fixed point result for a general contractive condition in the context of complex valued metric spaces. Also, some important corollaries under this contractive condition are obtained. As an application, we find a unique solution for Urysohn integral equations, and some illustrative examples are given to support our obtaining results. Our results extend and generalize the results of Azam et al. Previous known related results in the literarure and some other known results in the literature. Full article

Essential oils and hydrosols of two cultivars of muscadine grapes (Muscadinia rotundifolia (Michx.) Small.) were obtained by hydro-distillation of flowers and berry skins. Twenty-three volatile compounds were identified in essential oils from the muscadine flowers, and twenty volatiles in their corresponding hydrosols. The composition of volatiles in berry skins differed significantly from that of the vine flowers. The antioxidant potential of investigated essential oils and hydrosols was evaluated using five in vitro assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) method, TEAC (Trolox equivalent antioxidant capacity), FRAP (Ferric reducing antioxidant power), CUPRAC (cupric ion reducing antioxidant capacity), and NO (nitric oxide radical scavenging assay). The essential oils from the flowers of both cultivars showed the strongest antioxidant power, whereas the hydrosols were the significantly less active. All investigated essential oils showed very weak antibacterial activities against Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. However, the essential oils from the flowers of both cultivars showed moderate antifungal activities against Candida albicans, which were stronger for the oil from “Carlos” (white muscadine cultivar). To the best of our knowledge, this is the first report on obtaining and characterizing essential oils and hydrosols from muscadine grapes. This study demonstrated the variations in aromatic compounds accumulated in flowers and mature berry skins of muscadine grapes, and evaluated their possible antioxidant and antimicrobial activities. The presented results will be the base for future research, focused on a better understanding of the molecular and regulatory mechanisms involved in aromatic compound biosynthesis and accumulation in muscadine grapes. Full article

We demonstrate the fabrication of single-mode optical waveguides on lithium niobate on an insulator (LNOI) by optical patterning combined with chemomechanical polishing. The fabricated LNOI waveguides had a nearly symmetric mode profile of ~2.5 µm mode field size (full-width at half-maximum). We developed a high-precision measurement approach by which single-mode waveguides were characterized to have propagation loss of ~0.042 dB/cm. Full article

The use of mineral water for therapeutic purposes has varied from century to century and from country to country. Its effectiveness depends on the individual experiences of the population and their cultural traditions. Usually, the waters recommended for dermatological treatment are hot springs that contain sulfur or more recently, silicon. The mechanisms by which mineral waters actuate in dermatological disorders are still not clear but it is believed that they involve thermal, mechanical, chemical, immunologic, and anti-oxidant reactions and enzymatic activity. The aim of this study is to characterize the thermal waters of Monfortinho, one of the oldest Portuguese spas, their potential use for the preparation of dermatological formulations (creams), and their effectiveness on the treatment of skin disorders (psoriasis and eczema). To accomplish this, cream formulations with different contents of thermal water were developed. The formulations were characterized in terms of thermal water analysis and physicochemical properties and their effects were studied by skin biometrics in adults (mean age of 54.3 years old) through skin hydration evaluation and evaluated in clinical studies on 22 patients with psoriasis and eczema. The results showed that all the formulations improved the skin hydration and have beneficial effects in relieving the symptoms of psoriasis and other disorders, but no significant differences were observed when thermal water was used (compared to laboratory ultra-pure water). Full article

Evapotranspiration (ET) is a critical component of the water and energy balances, and the number of remote sensing-based ET products and estimation methods has increased in recent years. Various aspects of remote sensing of ET are reported in 11 papers published in this special issue. The major research topics covered by this special issue include inter-comparison and performance evaluation of widely used one- and two-source energy balance models, a new dual-source model (Soil Plant Atmosphere and Remote Sensing Evapotranspiration, SPARSE), and a process-based model (ETMonitor); assessment of multi-source (e.g., remote sensing, reanalysis, and land surface model) ET products; development or improvement of data fusion frameworks to provide continuous daily ET at a high spatial resolution (field-scale or 30 m) by fusing the advanced space-borne thermal emission reflectance radiometer (ASTER), the moderate resolution imaging spectroradiometer (MODIS), and Landsat data; and investigating uncertainties in ET estimates using an ET ensemble composed of 36 land surface models and four diagnostic datasets. The effects of the differences among ET products on water resources and ecosystem management were also investigated. More accurate ET estimates and improved understanding of remotely sensed ET products can help maximize crop productivity while minimizing water loses and management costs. Full article