Journal Description
Veterinary Sciences
Veterinary Sciences
is an international, scientific, peer-reviewed, open access journal on veterinary sciences published monthly online by MDPI.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High visibility: indexed within Scopus, SCIE (Web of Science), PubMed, PMC, Embase, PubAg, AGRIS, and other databases.
- Journal Rank: JCR - Q2 (Veterinary Sciences) / CiteScore - Q2 (General Veterinary)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 16.7 days after submission; acceptance to publication is undertaken in 2.8 days (median values for papers published in this journal in the second half of 2022).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
Impact Factor:
2.518 (2021)
Latest Articles
Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in Cooperia in Ecuador
Vet. Sci. 2023, 10(6), 378; https://doi.org/10.3390/vetsci10060378 - 28 May 2023
Abstract
Anthelmintic resistance among GINs in cattle is a worldwide issue. Identifying the early signs of anthelmintic resistance (AR) is necessary to sustainably manage bovine parasitic infections. This study aimed to evaluate the resistance status of bovine parasitic nematodes against FBZ on a farm
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Anthelmintic resistance among GINs in cattle is a worldwide issue. Identifying the early signs of anthelmintic resistance (AR) is necessary to sustainably manage bovine parasitic infections. This study aimed to evaluate the resistance status of bovine parasitic nematodes against FBZ on a farm with a known history of broad-spectrum anthelmintic usage in Ecuador. FBZ efficacy was analyzed using a fecal egg count reduction test (FECR test) and β-tubulin 1 mutation identification in Cooperia spp., the dominant nematode parasite identified before and after treatment. According to the FECR test, the nematode population was susceptible to FBZ. After amplifying and cloning the β-tubulin 1 of Cooperia spp., an F200Y mutation was found in 43% of the pooled larva coproculture after treatment. This study reports, for the first time, the presence of F200Y resistance-conferring mutation in Cooperia spp. in Ecuador. Although the nematode population was phenotypically susceptible to FBZ, the presence of F200Y suggests the existence of resistance in the early stages. Our findings highlight the need to implement alternative control strategies for parasitic infections besides broad-spectrum anthelmintics.
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(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)
Open AccessArticle
Outcomes in Dogs with Multiple Sites of Cervical Intervertebral Disc Disease Treated with Single Ventral Slot Decompression
Vet. Sci. 2023, 10(6), 377; https://doi.org/10.3390/vetsci10060377 - 28 May 2023
Abstract
In dogs with acute myelopathy but showing multiple sites of spinal compression from intervertebral disc disease (IVDD) on imaging, one approach is surgical decompression of the single acute disc extrusion while ignoring other previously extruded or protruded discs. However, little is known regarding
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In dogs with acute myelopathy but showing multiple sites of spinal compression from intervertebral disc disease (IVDD) on imaging, one approach is surgical decompression of the single acute disc extrusion while ignoring other previously extruded or protruded discs. However, little is known regarding the outcomes of this approach. This study described the outcomes and investigated prognostic factors in 40 dogs with multiple sites of cervical disc extrusion or protrusion on MRI who underwent ventral slot decompression for the single acute disc. The overall recovery rate was 97.5%. The median recovery time was seven days. The number of affected discs (including disc extrusion and protrusion) and the presence and number of the affected discs causing severe spinal compression did not influence the 30-day outcome. Compared with 23 dogs with single disc extrusion treated surgically, the recovery time and outcomes were similar between the two groups. The total number of affected discs was not associated with recovery time or outcomes. In conclusion, if an acute disc could be identified, ventral slot decompression targeting the single acute disc is a viable management approach for dogs with an acute presentation but diagnosed with multiple sites of spinal cord compression from IVDD.
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(This article belongs to the Special Issue Neurology and Neurosurgery in Small Animals)
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Open AccessCase Report
Thoracic Tumor Associated with a Unilateral Empyema in a Beef Cow: A Case Report
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, , , , , , and
Vet. Sci. 2023, 10(6), 376; https://doi.org/10.3390/vetsci10060376 - 27 May 2023
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Tumors in cows are not frequently reported in the literature. They often represent unusual findings in live animals and are incidental at slaughter with rare positive therapeutic outcomes for farmers. A 9-year-old beef cow was referred to the hospital of ruminants of the
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Tumors in cows are not frequently reported in the literature. They often represent unusual findings in live animals and are incidental at slaughter with rare positive therapeutic outcomes for farmers. A 9-year-old beef cow was referred to the hospital of ruminants of the National Veterinary School of Toulouse, France. The cow started to become sick 10 days prior, and major symptoms were anorexia, arched back, tachycardia, and tachypnea associated with significantly attenuated cardiac and pulmonary sounds upon right-sided auscultation. After specific investigations, a thoracic sarcoma associated with unilateral empyema was diagnosed. The empyema was treated, and supportive treatment was only performed for the tumor. Although the sarcoma remained, clinical improvement was significant, and the cow went back to her farm of origin. After the end of the withdrawal period, the cow recovered clinically but was culled by the owners for economic reasons. The present case report offers a continuum from the initial clinical signs motivating specific investigations to interesting laboratory findings, which were confirmed post-mortem.
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Open AccessCase Report
Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (Mustela putorius furo)
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, , , , , , , , , and
Vet. Sci. 2023, 10(6), 375; https://doi.org/10.3390/vetsci10060375 - 26 May 2023
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Canine distemper is a contagious and severe systemic viral disease that affects domestic and wild carnivores worldwide. In this study, two adult female ferrets (Mustela putorius furo) were evaluated for cutaneous lesions. Scab, fur, and swab samples from the external auditory
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Canine distemper is a contagious and severe systemic viral disease that affects domestic and wild carnivores worldwide. In this study, two adult female ferrets (Mustela putorius furo) were evaluated for cutaneous lesions. Scab, fur, and swab samples from the external auditory canal, cutaneous lesions, and scrapings were analyzed. Canine distemper virus (CDV)-positive samples underwent RT-PCR/RFLP with the restriction enzyme PsiI, and the hemagglutinin gene sequence was obtained. According to the restriction enzyme and sequence analyses, the viral strains were typed as CDV field strains that are included within the Europe lineage and distinct from those including vaccinal CDV strains. The sequence analysis showed the highest nucleotide identity rates in older Europe lineage CDV strains collected from dogs and a fox in Europe. This study is the first to report on CDV infection in ferrets in southern Italy and contributes to the current knowledge about natural CDV infection in this species. In conclusion, vaccination remains crucial for preventing the disease and counteracting cross-species infection. Molecular biology techniques can enable the monitoring of susceptible wild animals by ensuring the active surveillance of CDV spread.
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Open AccessArticle
Flow Cytometric Features of B- and T-Lmphocytes in Reactive Lymph Nodes Compared to Their Neoplastic Counterparts in Dogs
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, , , , and
Vet. Sci. 2023, 10(6), 374; https://doi.org/10.3390/vetsci10060374 - 26 May 2023
Abstract
An in-depth knowledge of non-neoplastic patterns is fundamental to diagnose neoplasia. In the present study, we described the flow cytometric (FC) cell size (FSC) and fluorescence intensity (MFI) of B- and T-lymphocytes in 42 canine reactive lymph nodes and 36 lymphomas. Proliferative activity
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An in-depth knowledge of non-neoplastic patterns is fundamental to diagnose neoplasia. In the present study, we described the flow cytometric (FC) cell size (FSC) and fluorescence intensity (MFI) of B- and T-lymphocytes in 42 canine reactive lymph nodes and 36 lymphomas. Proliferative activity (Ki67%) in reactive lymph nodes was also reported. Reactive lymph nodes were composed of a mixed population of small and large T (CD5+) and B (CD21+) cells. Small T-cells were larger in size than small B-cells, and large T-cells were larger than large B-cells. Small T-cells were composed of CD5+CD21− and CD5+CD21+dim subpopulations. Large B-cells were <20% in reactive lymph nodes and >20% in lymphomas and showed a higher FSC in lymphomas than in reactive lymph nodes. Large T-cells were <4% in reactive lymph nodes and >4% in lymphomas and showed a higher CD5 MFI in lymphomas (if expressed) compared to reactive lymph nodes. A subset of CD5+CD21+dim lymphocytes was recognized in addition to CD5+CD21- and CD5−CD21+ cells. In T-zone lymphomas, neoplastic cells had higher FSC and CD21 MFI values than small CD5+CD21+dim cells in reactive lymph nodes. Ki67% values were higher than those reported in normal lymph nodes, and largely overlapped with those reported in low-grade lymphomas and partially in high-grade lymphomas. Our results may contribute to making a less operator-dependent FC differential between lymphoma and reactive lymph nodes.
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(This article belongs to the Special Issue Advances in Canine and Feline Lymphoma)
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Open AccessArticle
Incorporation of Testicular Ultrasonography and Hair Steroid Concentrations in Bull Breeding Soundness Evaluation
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, , , , , , , and
Vet. Sci. 2023, 10(6), 373; https://doi.org/10.3390/vetsci10060373 - 25 May 2023
Abstract
Testicular ultrasonography and steroid concentrations (cortisol, dehydroepiandrosterone sulfate (DHEA-S), cortisol/DHEA-S ratio, testosterone) in hair were examined for their utility in the bull breeding soundness evaluation (BBSE). Beef and dairy bulls (n = 16; 2.7 ± 0.4 years old; body condition score 3.2
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Testicular ultrasonography and steroid concentrations (cortisol, dehydroepiandrosterone sulfate (DHEA-S), cortisol/DHEA-S ratio, testosterone) in hair were examined for their utility in the bull breeding soundness evaluation (BBSE). Beef and dairy bulls (n = 16; 2.7 ± 0.4 years old; body condition score 3.2 ± 0.1) of five breeds were maintained under the same conditions at an accredited semen collection center. Bulls underwent routine semen collection twice weekly for 12 weeks and semen was processed and cryopreserved. Ultrasonography and hair sampling were undertaken at the last semen collection. Bulls with homogeneous testicular parenchyma (n = 8) had a higher (p < 0.05) percentage of motile sperm post-thawing compared with bulls with heterogeneous parenchyma (n = 8). There were no differences (p > 0.05) in the hair concentrations of cortisol, DHEA-S, and testosterone between bulls with homogeneous and heterogeneous parenchyma. In bulls with homogeneous parenchyma, hair DHEA-S concentration was positively correlated with percentage motile sperm (R2 = 0.76), progressively motile sperm (R2 = 0.70), and motility yield (R2 = 0.71). The findings indicate that the integration of testicular ultrasonography and hair DHEA-S status in the BBSE could provide a more comprehensive assessment of indicative fertility in bulls. Additionally, ultrasonography can be used in the BBSE when the evaluation of semen parameters is not available.
Full article
(This article belongs to the Special Issue Endocrine and Metabolic Regulation in Male Animal Reproduction)
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Open AccessReview
Long-Acting Opioid Analgesics for Acute Pain: Pharmacokinetic Evidence Reviewed
by
and
Vet. Sci. 2023, 10(6), 372; https://doi.org/10.3390/vetsci10060372 - 24 May 2023
Abstract
Long-acting injectable (LAI) opioid formulations mitigate the harm profiles and management challenges associated with providing effective analgesia for animals. A single dose of a long-acting opioid analgesic can provide up to 72 h of clinically relevant pain management. Yet, few of these new
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Long-acting injectable (LAI) opioid formulations mitigate the harm profiles and management challenges associated with providing effective analgesia for animals. A single dose of a long-acting opioid analgesic can provide up to 72 h of clinically relevant pain management. Yet, few of these new drugs have been translated to products for veterinary clinics. Regulatory pathways allow accelerated drug approvals for generic and biosimilar drugs. These pathways depend on rigorous evidence for drug safety and pharmacokinetic evidence demonstrating bioequivalence between the new and the legacy drug. This report reviews the animal PK data associated with lipid and polymer-bound buprenorphine LAI formulations. Buprenorphine is a widely used veterinary opioid analgesic. Because of its safety profile and regulatory status, buprenorphine is more accessible than morphine, methadone, and fentanyl. This review of PK studies coupled with the well-established safety profile of buprenorphine suggests that the accelerated approval pathways may be available for this new family of LAI veterinary pharmaceuticals.
Full article
(This article belongs to the Special Issue Pharmacokinetics and Pharmacodynamics in Animal Clinical Treatment)
Open AccessArticle
Femoral Neck Thickness Index as an Indicator of Proximal Femur Bone Modeling
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, , , , , , , , and
Vet. Sci. 2023, 10(6), 371; https://doi.org/10.3390/vetsci10060371 - 24 May 2023
Abstract
The alteration in the shape of the femoral neck is an important radiographic sign for scoring canine hip dysplasia (CHD). Previous studies have reported that the femoral neck thickness (FNT) is greater in dogs with hip joint dysplasia, becoming progressively thicker with disease
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The alteration in the shape of the femoral neck is an important radiographic sign for scoring canine hip dysplasia (CHD). Previous studies have reported that the femoral neck thickness (FNT) is greater in dogs with hip joint dysplasia, becoming progressively thicker with disease severity. The main objective of this work was to describe a femoral neck thickness index (FNTi) to quantify FNT and to study its association with the degree of CHD using the Fédération Cynologique Internationale (FCI) scheme. A total of 53 dogs (106 hips) were randomly selected for this study. Two examiners performed FNTi estimation to study intra- and inter-examiner reliability and agreement. The paired t-test, the Bland-Altman plots, and the intraclass correlation coefficient showed excellent agreement and reliability between the measurements of the two examiners and the examiners’ sessions. All joints were scored in five categories by an experienced examiner according to FCI criteria. The results from examiner 1 were compared between FCI categories. Hips that were assigned an FCI grade of A (n = 19), B (n = 23), C (n = 24), D (n = 24), and E (n = 16) had a mean ± standard deviation FNTi of 0.809 ± 0.024, 0.835 ± 0.044, 0.868 ± 0.022, 0.903 ± 0.033, and 0.923 ± 0.068, respectively (ANOVA, p < 0.05). Therefore, these results show that FNTi is a parameter capable of evaluating proximal femur bone modeling and that it has the potential to enrich conventional CHD scoring criteria if incorporated into a computer-aided diagnosis capable of detecting CHD.
Full article
(This article belongs to the Section Veterinary Surgery)
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Open AccessArticle
Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle
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, , , , , and
Vet. Sci. 2023, 10(6), 370; https://doi.org/10.3390/vetsci10060370 - 23 May 2023
Abstract
This study looked at genetic polymorphisms and transcript levels of immune, antioxidant, and erythritol-related markers for postparturient endometritis prediction and tracking in Holstein dairy cows. One hundred and thirty female dairy cows (65 endometritis affected and 65 apparently healthy) were used. Nucleotide sequence
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This study looked at genetic polymorphisms and transcript levels of immune, antioxidant, and erythritol-related markers for postparturient endometritis prediction and tracking in Holstein dairy cows. One hundred and thirty female dairy cows (65 endometritis affected and 65 apparently healthy) were used. Nucleotide sequence variations between healthy and endometritis-affected cows were revealed using PCR-DNA sequencing for immune (TLR4, TLR7, TNF-α, IL10, NCF4, and LITAF), antioxidant (ATOX1, GST, and OXSR1), and erythritol-related (TKT, RPIA, and AMPD1) genes. Chi-square investigation exposed a noteworthy variance amongst cow groups with and without endometritis in likelihood of dispersal of all distinguished nucleotide variants (p < 0.05). The IL10, ATOX1, and GST genes were expressed at substantially lower levels in endometritis-affected cows. Gene expression levels were considerably higher in endometritis-affected cows than in resistant ones for the genes TLR4, TLR7, TNF-α, NCF4, LITAF, OXSR1, TKT, RPIA, and AMPD1. The sort of marker and vulnerability or resistance to endometritis had a significant impact on the transcript levels of the studied indicators. The outcomes might confirm the importance of nucleotide variants along with gene expression patterns as markers of postparturient endometritis susceptibility/resistance and provide a workable control plan for Holstein dairy cows.
Full article
(This article belongs to the Special Issue Current Research in Bovine Uterine Infection)
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Open AccessArticle
Evaluation of a Phytogenic Feed Supplement Containing Carvacrol and Limonene on Sheep Performance and Parasitological Status on a Hungarian Milking Sheep Farm
Vet. Sci. 2023, 10(6), 369; https://doi.org/10.3390/vetsci10060369 - 23 May 2023
Abstract
There is currently worldwide interest in phytogenic feed supplements (PFSs) because they can lead to improved animal production. The scope of the present study was to observe the impact of a feed supplement containing carvacrol (CAR) and limonene (LIM) on the performance and
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There is currently worldwide interest in phytogenic feed supplements (PFSs) because they can lead to improved animal production. The scope of the present study was to observe the impact of a feed supplement containing carvacrol (CAR) and limonene (LIM) on the performance and parasitological status of sheep. The feed supplement decreased the plasma levels of β-hydroxybutyrate (p < 0.001), triglycerides (p = 0.014), nonesterified fatty acids (p = 0.021), and fructosamine (p = 0.002) in lactating ewes after 42 days of supplementation, while the average live weight (p = 0.002) and average daily weight gain (p = 0.001) of their twin suckling lambs increased significantly by the end of the study. In another experiment, fattening lambs fed the same supplement showed a decrease in fecal egg number of gastrointestinal nematodes (p = 0.02) but no differences in live weight, average daily gain, or mean number of Haemonchus contortus nematodes in the abomasum. The results highlighted that the inclusion of carvacrol and limonene in the feed of lactating ewes effectively increased the weight gain of the suckling lambs, presumably due to the ewes’ improved energy, but further studies are needed to elucidate the effects of carvacrol and limonene against gastrointestinal parasites.
Full article
(This article belongs to the Section Nutritional and Metabolic Diseases in Veterinary Medicine)
Open AccessArticle
Effect of Nutritional Flushing Using Long-Term Energy and Protein Supplementation on Growth Performance and Reproductive Parameters of Doyogena Ewes in Ethiopia
Vet. Sci. 2023, 10(6), 368; https://doi.org/10.3390/vetsci10060368 - 23 May 2023
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The objective of the study was to establish the effect of appropriate supplementation days (days -21 to +7) using four isonitrogenous (14.7% CP) diets balanced to provide low (Lo-ME) or high (Hi-ME) metabolizable energy on the body condition score (BCS), body weight (BW)
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The objective of the study was to establish the effect of appropriate supplementation days (days -21 to +7) using four isonitrogenous (14.7% CP) diets balanced to provide low (Lo-ME) or high (Hi-ME) metabolizable energy on the body condition score (BCS), body weight (BW) change, and reproductive performances of sheep. Thirty-five Doyogena ewes (27.71 ± 2.87 kg, 2–5 years of age, BCS of 2.0–2.5) grazing on natural pasture were randomly assigned to supplementary treatments consisting of combinations of enset leaf (EL) and commercial concentrate (CC): T0 (control), T1 (250 g EL + 500 g CC: Lo-ME), T2 (400 g EL + 500 g CC; Hi-ME), T3 (500 g EL + 400 g CC; Hi-ME), and T4 (500 g EL + 250 g CC; Lo-ME). The estrous cycle was synchronized with one intramuscular injection, 5 mg PGF2α, prior to artificial insemination. The dry matter (DM) from the pasture provided 1.10–1.46 kg/day, which corresponds to the DM requirements of the ewes until late gestation. However, the pasture provided a protein content of 9.52%, which was insufficient for breeding, mid-gestation, and gestation, requiring minimums of 16.1%, 13.1%, and 14.8%, respectively. The pasture could only provide enough energy for breeding ewes with a BW of up to 30 kg. The energy provided by pasture was insufficient for ewes weighing > 30 kg at mid-gestation and gestation, providing 6.9–9.2 MJ/day, below the requirement of 11.92–16.32 MJ/day required for mid-gestation and gestation. The energy was not sufficient for large ewes weighing > 40 kg. Supplementary diets T1–T4 provided DM in the range of 1.7–2.29 kg/day. This was sufficient for AI, mid-gestation, and gestation phases. Dietary supplements increased (p < 0.01) BW during breeding and mid-gestation. During lambing, T2 and T3 increased BW (p < 0.05) compared to T4 and T1. T4 had a similar effect (p > 0.05) on BW during lambing. T1, T2, and T3 significantly increased BCS (p < 0.05). T2 and T3 increased (p < 0.05) BCS at mid-gestation, but only T2 significantly increased BCD (p < 0.05) during lambing. All dietary supplements resulted in a shorter (p < 0.05) time to the resumption of estrous and the length of estrous (p < 0.05). T1, T2, and T3 resulted in a stronger estrous response (p < 0.05). Dietary supplements enhanced (p < 0.05) the conception rate and fecundity rate. The conception rate was highest in T2 and T3 at 85.7% and 83.3%, respectively. T2 had the highest fecundity rate at 151.7% (p < 0.05). Dietary supplementation increased the rate of lambing (LR), litter size (LS), and weight of lambs at birth (LBW). The LR for treatments T2, T3, and T4 was 100% versus 66.7% in the control. T1 and T2 significantly increased (p < 0.05) LS, but T4 had a similar LS to the control. Dietary supplements T1, T3, and T4 tended to increase (p < 0.05) LBW, but T2 increased LBW significantly (p < 0.05). Supplementation (T2, T3) with 400 g enset + 500 g CC and 500 g enset + 400 g CC are promising feed supplements to increase the reproductive capacities of Doyogena ewes in Ethiopia. Energy is as important to ewe flushing as protein.
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Open AccessArticle
Are They Thinking Differently? The Perceptions and Differences in Medical Disputes between Veterinarians and Clients
Vet. Sci. 2023, 10(5), 367; https://doi.org/10.3390/vetsci10050367 - 22 May 2023
Abstract
Medical disputes in veterinary practices are widespread; yet, a limited amount of research has been conducted to investigate the factors contributing to medical disputes. This study examined veterinarians’ and clients’ perceptions regarding risk factors and possible solutions to medical disputes. A total of
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Medical disputes in veterinary practices are widespread; yet, a limited amount of research has been conducted to investigate the factors contributing to medical disputes. This study examined veterinarians’ and clients’ perceptions regarding risk factors and possible solutions to medical disputes. A total of 245 respondents from Taiwan, including 125 veterinarians and 120 clients, completed an electronic self-administered, semi-structured questionnaire in 2022. The questionnaire covered six dimensions: medical skills, complaint management, the attitudes of stakeholders during interactions, medical expenses, clients’ perspectives, and communication modes. The results highlighted significant differences in the perceptions of risk factors for inducing medical disputes and possible solutions between clients and veterinarians in veterinary practice. First, young veterinarians and clients perceived medical skills as the highest risk factor for inducing medical disputes, while experienced veterinarians disagreed (p < 0.001). In addition, veterinarians with medical dispute experience identified stakeholders’ attitudes during interactions as the top contributing factor. Second, regarding possible solutions, all veterinarians preferred offering clients cost estimates and cultivating empathy and compassion towards them. On the other hand, clients underscored the importance of obtaining informed consent for treatments and expenses and suggested that veterinarians should supply comprehensive written information to facilitate this process. This study underlies the importance of understanding stakeholders’ perceptions to mitigate medical disputes and advocates for improved communication education and training for young veterinarians. These findings provide valuable insights for veterinarians and clients, contributing to preventing and managing medical disputes in veterinary practices.
Full article
(This article belongs to the Special Issue Veterinary Medical Education: Challenges and Perspectives)
Open AccessArticle
Antimicrobial Use in Canadian Cow–Calf Herds
Vet. Sci. 2023, 10(5), 366; https://doi.org/10.3390/vetsci10050366 - 20 May 2023
Abstract
Despite growing concern surrounding antimicrobial use (AMU) and the importance of cow–calf herds to the Canadian livestock industry, surveillance of AMU in cow–calf herds to inform antimicrobial stewardship programs has been sporadic. Producers from the Canadian Cow–Calf Surveillance Network (87%, 146/168) provided data
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Despite growing concern surrounding antimicrobial use (AMU) and the importance of cow–calf herds to the Canadian livestock industry, surveillance of AMU in cow–calf herds to inform antimicrobial stewardship programs has been sporadic. Producers from the Canadian Cow–Calf Surveillance Network (87%, 146/168) provided data and almost all reported AMU in at least one animal (99%, 145/146 herds) in 2019–2020. The most common reasons for AMU were treatment of respiratory disease in nursing calves in 78% of herds and neonatal diarrhea in 67% of herds, as well as for lameness in cows in 83% of herds. However, most herds treated <5% of animals for these reasons. Less than 2.5% of herds treated more than 30% of calves for either bovine respiratory disease or neonatal diarrhea and no herds treated more than 30% of cows for lameness. The most frequently reported antimicrobial was oxytetracycline in 81% of herds, followed by florfenicol in 73% of herds. Antimicrobials with very high importance to human health, such as ceftiofur, were used at least once by 20% of herds but were only used in >30% of nursing calves from one herd. Similarly, while 56% of herds used macrolides at least once, within-herd use was the highest in nursing calves where <4% of herds reported use in >30% of animals. Herds using artificial insemination and calving in the winter were more likely (p = 0.05) to treat >5% of nursing calves for respiratory disease, suggesting the importance of vaccination programs for herds at risk. Overall, AMU was similar to previous Canadian studies; however, the percentage of herds using macrolides had increased from a comparable study in 2014.
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Open AccessArticle
Secondary Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2) Infection Augments Inflammatory Responses, Clinical Outcomes, and Pathogen Load in Glaesserella-parasuis-Infected Piglets
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, , , , , , , , , , and
Vet. Sci. 2023, 10(5), 365; https://doi.org/10.3390/vetsci10050365 - 20 May 2023
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Glaesserella parasuis (Gps), Gram-negative bacteria, are a universal respiratory-disease-causing pathogen in swine that colonize the upper respiratory tract. Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2HP-PRRSV2) and Gps coinfections are epidemics in China, but little is known about the influence of concurrent
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Glaesserella parasuis (Gps), Gram-negative bacteria, are a universal respiratory-disease-causing pathogen in swine that colonize the upper respiratory tract. Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP-PRRSV2HP-PRRSV2) and Gps coinfections are epidemics in China, but little is known about the influence of concurrent coinfection on disease severity and inflammatory responses. Herein, we studied the effects of secondary HP-PRRS infection on clinical symptoms, pathological changes, pathogen load, and inflammatory response of Gps coinfection in the upper respiratory tract of piglets. All coinfected piglets (HP-PRRSV2 + Gps) displayed fever and severe lesions in the lungs, while fever was present in only a few animals with a single infection (HP-PRRSV2 or Gps). Additionally, HP-PRRSV2 and Gps loading in nasal swabs and blood and lung tissue samples was significantly increased in the coinfected group. Necropsy data showed that coinfected piglets suffered from severe lung damage and had significantly higher antibody titers of HP-PRRSV2 or Gps than single-infected piglets. Moreover, the serum and lung concentrations of inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8) were also significantly higher in coinfected piglets than in those infected with HP-PRRSV2 or Gps alone. In conclusion, our results show that HP-PRRSV2 promotes the shedding and replication of Gps, and their coinfection in the upper respiratory tract aggravates the clinical symptoms and inflammatory responses, causing lung damage. Therefore, in the unavoidable situation of Gps infection in piglets, necessary measures must be made to prevent and control secondary infection with HP-PRRSV2, which can save huge economic losses to the pork industry.
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Open AccessArticle
Effects of Black Soldier Fly Larvae (Hermetia illucens Larvae) Meal on the Production Performance and Cecal Microbiota of Hens
Vet. Sci. 2023, 10(5), 364; https://doi.org/10.3390/vetsci10050364 - 19 May 2023
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The effects of Hermetia illucens larvae meal (HILM) as a feed supplement on production performance and cecal microflora were studied in 900 Hy-line Brown laying hens. Laying hens (60 weeks old) were randomly divided into four groups. Each group had five replicates, and
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The effects of Hermetia illucens larvae meal (HILM) as a feed supplement on production performance and cecal microflora were studied in 900 Hy-line Brown laying hens. Laying hens (60 weeks old) were randomly divided into four groups. Each group had five replicates, and each replicate had 45 hens. The control group was fed with a corn–soybean-based diet, and the experimental groups were fed with 1% HILM, 2% HILM, or 3% HILM. Results were as follows: (1) With the increase in HILM level, the laying rate increased linearly (p ≤ 0.05), and the feed/egg and cracked-egg rate decreased linearly (p ≤ 0.05). (2) Community composition analysis showed that the dominant bacteria in each group were Bacteroidetes and Firmicutes, followed by Actinobacteria and Proteobacteria, which accounted for more than 97% of 16S rRNA gene sequence of the total cecal bacteria. (3) Alpha diversity analysis at the operational taxonomic unit classification level showed that the HILM-addition groups had higher community richness and community diversity than the control group. (4) Principal co-ordinates analysis showed that the cecum samples in each group were significantly separated (p ≤ 0.05). At the phylum level, the relative abundance of Bacteroidetes in the HILM addition groups was significantly lower than that in the control group (p < 0.001), and the relative abundance of Firmicutes in the HILM addition groups was significantly higher than that in the control group (p < 0.001). In conclusion, dietary HILM supplementation had a significant effect on the production performance and cecal microflora of laying hens at the late laying period under the conditions of this experiment but had no adverse effect on the intestinal dominant flora.
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Open AccessArticle
Serum Bicarbonate Deficiency in Dogs with Acute and Chronic Kidney Disease
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, , , , and
Vet. Sci. 2023, 10(5), 363; https://doi.org/10.3390/vetsci10050363 - 19 May 2023
Abstract
Serum bicarbonate deficiency is a disorder frequently found in human patients with acute (AKI) and chronic (CKD) kidney disease, due to abnormalities in kidney generation and reabsorption of bicarbonate. Although alkali supplementation is usually performed in both humans and veterinary CKD patients, data
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Serum bicarbonate deficiency is a disorder frequently found in human patients with acute (AKI) and chronic (CKD) kidney disease, due to abnormalities in kidney generation and reabsorption of bicarbonate. Although alkali supplementation is usually performed in both humans and veterinary CKD patients, data regarding the frequency of bicarbonate disorders in AKI and CKD dogs are scarce. The aim of the present study is to assess the frequency and the severity of bicarbonate deficiency of dogs affected by AKI, acute chronic kidney disease (ACKD), and CKD, and to investigate its possible association with the IRIS grade/stage as well as with disorders of calcium phosphate metabolism. A retrospective evaluation of the serum biochemical panels of all dogs with diagnoses of AKI, ACKD, and CKD referred to the nephrology and urology service of the Veterinary Teaching Hospital of the University of Pisa, between January 2014 and January 2022, was performed. Bicarbonate deficiency was defined as serum bicarbonate < 22 mmol/L and classified as moderate (between 18 and 22 mmol/L) or severe (<18 mmol/L). Serum bicarbonate deficiency was found in 397/521 dogs (76%), of which 142/397 (36%) showed moderate deficiency, and 255/397 (64%) severe deficiency. Dogs with AKI and ACKD showed a significantly higher frequency of bicarbonate deficiency (p = 0.004) and severe forms compared to CKD dogs (p = 0.02). In AKI and ACKD dogs, a negative linear correlation was found between serum bicarbonate and serum creatinine, urea, and phosphate. The frequency of bicarbonate deficiency was higher in the later stages of the disease in both AKI (p = 0.01), ACKD (p = 0.0003), and CKD dogs (p = 0.009). Dogs with serum CaxP ≥ 70 mg2/dL2 showed a higher frequency of bicarbonate deficiency (p = 0.01) and showed severe forms (p = 0.01) compared to dogs with CaxP < 70 mg2/dL2. Serum bicarbonate deficiency seems to be a very frequent disorder in both AKI, ACKD, and CKD dogs, with an increasing frequency and severity in more advanced stages of kidney disease. The higher frequency and severity of bicarbonate deficiency in AKI and ACKD may be caused by a more severe and sudden loss of kidney function, or extra-renal factors. Finally, the association between frequency and severity of bicarbonate deficiency and abnormal CaxP may suggest a potential connection between metabolic acidosis and bone mineral disorders.
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(This article belongs to the Section Veterinary Internal Medicine)
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Open AccessArticle
Exploring the Enteric Virome of Cats with Acute Gastroenteritis
by
, , , , , and
Vet. Sci. 2023, 10(5), 362; https://doi.org/10.3390/vetsci10050362 - 18 May 2023
Abstract
Viruses are a major cause of acute gastroenteritis (AGE) in cats, chiefly in younger animals. Enteric specimens collected from 29 cats with acute enteritis and 33 non-diarrhoeic cats were screened in PCRs and reverse transcription (RT) PCR for a large panel of enteric
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Viruses are a major cause of acute gastroenteritis (AGE) in cats, chiefly in younger animals. Enteric specimens collected from 29 cats with acute enteritis and 33 non-diarrhoeic cats were screened in PCRs and reverse transcription (RT) PCR for a large panel of enteric viruses, including also orphan viruses of recent identification. At least one viral species, including feline panleukopenia virus (FPV), feline enteric coronavirus (FCoV), feline chaphamaparvovirus, calicivirus (vesivirus and novovirus), feline kobuvirus, feline sakobuvirus A and Lyon IARC polyomaviruses, was detected in 66.1% of the samples.. Co-infections were mainly accounted for by FPV and FCoV and were detected in 24.2% of the samples. The virome composition was further assessed in eight diarrhoeic samples, through the construction of sequencing libraries using a sequence-independent single-primer amplification (SISPA) protocol. The libraries were sequenced on Oxford Nanopore Technologies sequencing platform. A total of 41 contigs (>100 nt) were detected from seven viral families infecting mammals, included Parvoviridae, Caliciviridae, Picornaviridae, Polyomaviridae, Anelloviridae, Papillomaviridae and Paramyxoviridae, revealing a broad variety in the composition of the feline enteric virome.
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(This article belongs to the Special Issue Emerging Infectious Disease: Viral Enteric Pathogens with Zoonosis Potential in Domestic and Wild Animals II)
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Open AccessArticle
Paleopathological Changes in Animal Bones from Croatian Archaeological Sites from Prehistory to New Modern Period
by
, , , , and
Vet. Sci. 2023, 10(5), 361; https://doi.org/10.3390/vetsci10050361 - 18 May 2023
Abstract
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A special part of archaeology, so-called archaeozoopathology or veterinary paleopathology is dedicated to studies of paleopathological changes in animal remains and contributes to the knowledge of ancient veterinary medicine and the history of diseases. In our study, we analyze paleopathological changes determined by
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A special part of archaeology, so-called archaeozoopathology or veterinary paleopathology is dedicated to studies of paleopathological changes in animal remains and contributes to the knowledge of ancient veterinary medicine and the history of diseases. In our study, we analyze paleopathological changes determined by gross observation and diagnostic imaging in the animal material originating from eight archaeological sites in Croatia. A standard archaeozoological analysis was carried out and specimens with visually detected macrostructural changes were radiographed. In total, 50 animal remains with altered macrostructure were identified in the archaeozoological material excavated from 2010 to 2022 at eight archaeological sites in Croatia. According to the taxonomic analysis, most of the bones with macrostructural changes originated from cattle (N = 27, 54% of the total number of bones with macrostructural changes), followed by the bones of small ruminants (N = 12, 24%) and pigs (N = 8, 16%). The horse, carnivore and chicken were represented with one bone each (2%). Radiological examination showed that three samples (6%) had a regular bone macrostructure, i.e., no pathological changes were visible upon radiological examination. The majority (64%) of pathologically altered bones are a consequence of keeping/working, followed by traumatic causes (20%). Changes in the oral cavity were found in 10% of specimens. Our study showed that gross examination will continue to be the primary method for the identification of pathologically altered remains in archaeozoological material. However, diagnostic imaging techniques such as radiography should be implemented to confirm or exclude suspected alterations and to help the classification of the specimen by etiology.
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Alteration of the Gut Microbiota in Pigs Infected with African Swine Fever Virus
by
, , , , , , , and
Vet. Sci. 2023, 10(5), 360; https://doi.org/10.3390/vetsci10050360 - 18 May 2023
Abstract
The factors that influence the pathogenicity of African swine fever (ASF) are still poorly understood, and the host’s immune response has been indicated as crucial. Although an increasing number of studies have shown that gut microbiota can control the progression of diseases caused
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The factors that influence the pathogenicity of African swine fever (ASF) are still poorly understood, and the host’s immune response has been indicated as crucial. Although an increasing number of studies have shown that gut microbiota can control the progression of diseases caused by viral infections, it has not been characterized how the ASF virus (ASFV) changes a pig’s gut microbiome. This study analyzed the dynamic changes in the intestinal microbiome of pigs experimentally infected with the high-virulence ASFV genotype II strain (N = 4) or mock strain (N = 3). Daily fecal samples were collected from the pigs and distributed into the four phases (before infection, primary phase, clinical phase, and terminal phase) of ASF based on the individual clinical features of the pigs. The total DNA was extracted and the V4 region of the 16 s rRNA gene was amplified and sequenced on the Illumina platform. Richness indices (ACE and Chao1) were significantly decreased in the terminal phase of ASF infection. The relative abundances of short-chain-fatty-acids-producing bacteria, such as Ruminococcaceae, Roseburia, and Blautia, were decreased during ASFV infection. On the other hand, the abundance of Proteobacteria and Spirochaetes increased. Furthermore, predicted functional analysis using PICRUSt resulted in a significantly reduced abundance of 15 immune-related pathways in the ASFV-infected pigs. This study provides evidence for further understanding the ASFV–pig interaction and suggests that changes in gut microbiome composition during ASFV infection may be associated with the status of immunosuppression.
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(This article belongs to the Special Issue Emerging and Re-emerging Swine Viral Diseases)
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Comparison of Imaging Methods and Population Pattern in Dogs with Spinal Diseases in Three Periods between 2005 and 2022: A Retrospective Study
Vet. Sci. 2023, 10(5), 359; https://doi.org/10.3390/vetsci10050359 - 18 May 2023
Abstract
The aim of this study was the long-term comparison of the imaging methods used in dogs with neurologic diseases related to the spine and spinal cord. We also compared the occurrence of neurological diseases according to the localization, gender, age, and breed. As
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The aim of this study was the long-term comparison of the imaging methods used in dogs with neurologic diseases related to the spine and spinal cord. We also compared the occurrence of neurological diseases according to the localization, gender, age, and breed. As the availability of magnetic resonance imaging (MRI) has increased over the years, resulting in increased diagnostic and therapeutic success rates, the study was divided into three time periods (2005–2014, 2015–2018, and 2019–2022). Our results suggest changes in the population structure of the dogs studied and changes in the use of diagnostic methods that directly or indirectly influence the choice and success rate of therapy. Our results may be of interest to owners, breeders, practicing veterinarians, and insurance companies.
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(This article belongs to the Section Veterinary Surgery)
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