Forests — Open Access Journal

Portuguese forests have always played an essential role in the socioeconomic development of national rural areas, but also in several forest-based industrial sectors, such as the cork, pulp and paper, and wood panels industries. In addition to these dominant sectors, there are also several other uses for forest timber, such as being the major raw materials to the production of furniture or devoted to the growing biomass pellets production industry. This review article presents the evolution of the forest industrial sector throughout the recent past, and its impact on the development of the rural environment, from a socioeconomic perspective, namely concerning the jobs and value-added creation, as well as the importance of the forest in national industrial development. It shows the importance of sustainable forest management for the development of the rural environment, as an essential sector for the creation of wealth and for the establishment of populations in the interior regions of the country. Full article

The aim of this study was to investigate the potential mixing effects on degradation of lignin and cellulose in mixed leaf litter from Pinus massoniana Lamb., Cupressus funebris Endl., and/or Quercus variabilis Bl., and elucidate the interactions with abiotic factors. The litter bag method was used in the field experiment, and the three predominant species in the Three Gorges Reservoir region were treated as single-, pair-, and tri-species combinations with equal proportions of litter mass. Lignin and cellulose losses in the litter treatments were measured, and the mixing effects were evaluated based on the sampling phase and decomposition period. At the end of the one-year decomposition period, mixing species increased lignin loss by 3.3% for the cypress + oak combination and cellulose loss by 3.9%, 1.8%, and 0.8% for the pine + oak, cypress + oak, and pine + cypress + oak combinations, respectively. The pine + oak and cypress + oak combinations exhibited greater lignin and cellulose loss than the tri-species mixture. Accelerated lignin degradation also apparently occurred in the pine + cypress combination as decomposition proceeded. Generalized linear models suggested that the investigated environmental factors (in terms of average temperature and cumulative precipitation) and changing litter quality (lignin, cellulose, and lignin/cellulose) had significant effects on nonadditive lignin loss, whereas only the changing litter quality factors significantly affected nonadditive cellulose loss. In summary, mixing two or three of the studied species alters cycling of recalcitrant substrates in plantations, and mixed planting with Quercus appears to strengthen both the lignin and cellulose degradation processes. Full article

The importance of trust has been widely acknowledged as a major antecedent and a constitutive element of information exchange in policy networks. The ultimate objective of the present article is to understand whether and how trust is a factor explaining patterns of limited information exchange between forestry and nature conservation actors in forest policy networks in Europe. Drawing upon analytical insights of actor-centered institutionalism (ACI) and building upon a qualitative network analysis (QNA) as a research strategy, the study focuses on the German forest policy network in order to provide deeper insights into the cooperative (i.e., generalized trust) and competitive (i.e., generalized distrust) orientations of forestry and nature conservation actors. The results reveal trust issues with respect to forest information, which can be framed according to the interests of forestry and nature conservation actors and used as a discursive weapon, softly steering them in a particular direction. The underlying causes of the trust issues are deeply rooted in conflicts of interests and power, preventing from exchanging forest information among forestry and nature conservation actors. It is therefore argued that forest information should be understood as a political asset rather than a neutral and objective form of expertise. This could explain persistent trust issues and conflicts among forestry and nature conservation actors when it comes to the exchange of forest information in the context of European forest policy networks. Full article

Wood, also designated as secondary xylem, is the major structure that gives trees and other woody plants stability for upright growth and maintains the water supply from the roots to all other plant tissues. Over recent decades, our understanding of the cellular processes of wood formation (xylogenesis) has substantially increased. Plants as sessile organisms face a multitude of abiotic stresses, e.g., heat, drought, salinity and limiting nutrient availability that require them to adjust their wood structure to maintain stability and water conductivity. Because of global climate change, more drastic and sudden changes in temperature and longer periods without precipitation are expected to impact tree productivity in the near future. Thus, it is essential to understand the process of wood formation in trees under stress. Many traits, such as vessel frequency and size, fiber thickness and density change in response to different environmental stimuli. Here, we provide an overview of our current understanding of how abiotic stress factors affect wood formation on the molecular level focussing on the genes that have been identified in these processes. Full article

To assess the effects of long-term intensive management on soil carbon cycle and microbial functional diversity, we sampled soil in Chinese hickory (Carya cathayensis Sarg.) stands managed intensively for 5, 10, 15, and 20 years, and in reference Chinese hickory–broad-leaved mixed forest (NMF) stands. We analyzed soil total organic carbon (TOC), microbial biomass carbon (MBC), and water-soluble organic carbon (WSOC) contents, applied ¹³C-nuclear magnetic resonance analysis for structural analysis, and determined microbial carbon source usage. TOC, MBC, and WSOC contents and the MBC to TOC ratios were lower in the intensively managed stands than in the NMF stands. The organic carbon pool in the stands managed intensively for twenty years was more stable, indicating that the easily degraded compounds had been decomposed. Diversity and evenness in carbon source usage by the microbial communities were lower in the stands managed intensively for 15 and 20 years. Based on carbon source usage, the longer the management time, the less similar the samples from the monospecific Chinese hickory stands were with the NMF samples, indicating that the microbial community compositions became more different with increased management time. The results call for changes in the management of the hickory stands to increase the soil carbon content and restore microbial diversity. Full article

Drought is the major factor that limits vegetation recovery in rocky desertification areas. The leaf carbon isotope (δ¹³C) value is related to plant water-use efficiency (WUE) and is of great significance in revealing the WUE characteristics of species in karst areas. Measurements of the δ¹³C value in plant leaves and the nutrient and water contents of lithologic soils were obtained for six woody species (cypress, Cupressus funebris Endl.; mansur shrub, Coriaria nepalensis Wall.; camphor, Cinnamomum bodinieri Levl.; birch, Betula luminifera H. Winkl.; alder, Alnus cremastogyne Burk. and dyetree, Platycarya longipes Wu.) planted in three different lithologic soil types (dolomite, dolomite sandstone, limestone) in the karst area of Guizhou Province. The results showed that C. funebris in the dolomite sandstone soil had the highest δ¹³C value (−27.19‰), whereas C. bodinieri in the limestone soil had the lowest δ¹³C value (−31.50‰). In terms of lithology, the average leaf δ¹³C values were −28.66‰ (dolomitic sandstone), −28.83‰ (dolomite), and −29.46‰ (limestone). The δ¹³C values of C. funebris and A. cremastogyne were significantly lower in the limestone soil than in the dolomite and dolomite sandstone soil, indicating that the WUE of some tree species is affected by soil conditions under different lithological development processes. Moreover, the relationship between the δ¹³C value in the leaves and the comprehensive soil conditions varied among the species, and the δ¹³C value was negatively correlated with the soil water content in all three soil types. Our study provides basic data on the composition characteristics of the δ¹³C value of tree species, which is beneficial for the selection of tree species for vegetation restoration and afforestation in karst areas. Full article

The ber fruit fly Carpomya vesuviana Costa (Diptera: Tephritidae) is the most destructive pests of Ziziphus spp. Carpomya vesuviana infestation causes great economic losses. We re-parameterized an existing CLIMEX model, and used the updated CliMond 30′ gridded resolution datasets within CLIMEX for the periods 1987–2016 and 2071–2100, representing historical and future climates, respectively, to predict the potential global distribution of the pest. Under the historical climate scenario, C. vesuviana had a wide climatically suitable distribution worldwide, from approximately 46° S to 50° N. Future climate change expanded the upper boundary of the potential distribution northward, and predicted that the pest would distribute approximately from 50° S to 60° N. Temperature was the primary determinant of the potential distribution of the pest among all driving variables. Irrigation was associated with a slight improvement in the climate favorability for the pest in some areas, including south-western North America, northern and southern Africa, and most of Oceania. The projections clarify the impacts of climate change on the potential global distribution of C. vesuviana, and are instructive for quarantine and management agencies for reducing economic damage caused by the fly and preventing expansion of C. vesuviana due to climate change. Full article

Western spruce budworm (WSBW) is a common defoliating insect that has caused extensive damage and mortality to a number of tree species across the western United States (US). Past studies have linked outbreaks of WSBW to increased moisture stress of host trees in the Northwest and decreased moisture stress in the Southwest. Our study analyzed seasonal drought stress metrics with WSBW outbreaks within Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) forests in the western US during 1997–2015. Superposed epoch analysis and defoliation area growth rates (representing insect population growth rates) were assessed to quantify the drought conditions associated with the initiation and continuation of outbreaks, respectively. We found that multiple years of drought occurred prior to and during outbreak initiation in the Northwest, and that outbreak initiation in the Southwest was associated with only weak drought or neutral conditions. During the outbreak continuation stage, there was a weak positive correlation between May moisture availability and defoliation area growth rates in the Southwest (R² = 0.12), but no clear relationship was identified in the Northwest. Increased frequency of summer droughts such as these expected from climate change may increase WSBW outbreaks and promote tree dieoff. Improved understanding of the role of different influences of drought and moisture availability across landscapes will lead to improved predictions and management of future outbreaks of WSBW. Full article

Research Highlights: Direct comparison of leaf litter decomposition rates between harsh soil conditions of degraded lands and adjacent “closer to natural” forest areas has not been done before. Background and Objectives: We aimed to fill this knowledge gap by determining the differences in amounts of carbon and nitrogen released by species-specific litter depending on decomposition rates in various stand and habitat conditions, which enables selection of the most ecologically and economically appropriate (for fast soil organic layer development) tree species for afforestation of reclaimed lands. Materials and Methods: The study was conducted on the external spoil heap of the “Bełchatów” lignite mine (Central Poland) and adjacent forests. In December 2013, we established a litterbag experiment beneath the canopies of birch and pine stands. We used litter of Alnusglutinosa (Gaertn.), Betulapendula (Roth), Pinussylvestris (L.), and Quercusrobur (L.) collected ex situ, which we installed (after oven-drying) beneath the canopies of eight stands. The experiment lasted for three years (with sampling of three-month intervals). Results: Harsh soil conditions of degraded lands are unfavorable for litter mineralization. It was found that 23%–74% of decomposed materials were mineralized in spoil heap stands, whereas in forest stands these amounts ranged from 35%–83%. Litter of Q. robur in birch stands on the spoil heap is predicted to take 12 years longer for total decomposition than in forest stands of the same species. This hinders organic carbon turnover and could result in elongation of the time for full biological and economic reclamation of degraded lands. On the other hand, decomposition of relatively fast decomposable litter (A. glutinosa and B. pendula) in pine stands on the spoil heap was faster than in pine stands in forest sites (17% and 13% faster, respectively). We did not observe this trend for decomposition of more recalcitrant litter types of P. sylvestris and Q. robur. Conclusions: The results show the value of selective choice of tree species for afforestation of post-mining areas to accelerate the development of technogenic soil substrates. We recommend introducing all tree species studied in the cluster form of admixtures as all of them could bring some profits in ecological and economical reclamation. Full article

Microbial communities and their associated enzyme activities play key roles in carbon cycling in ecosystems. Forest thinning is likely to change the soil properties and feedbacks on the structure and function of microbial communities, consequently affecting microbial regulation on the soil carbon process. However, few studies have focused on the mechanism of how thinning affects the quantity and stability of soil carbon. To reveal the influence of thinning on soil carbon and to explore the regulated key factors, this study was conducted in a pure Larix principis-rupprechtii Mayr plantation with different thinning intensity (light, medium, and high) in Shanxi province, China. Soil properties (soil pH, soil water content, soil organic carbon, and soil microbial biomass carbon) were measured. Meanwhile, soil microbial communities were examined with the method of phospholipid fatty acid (PLFA), and soil enzyme activities were measured as indicators of soil microbial functions. The results showed that medium and high thinning has positive effects on soil organic carbon, microbial biomass carbon, soil microbial abundance, and soil enzyme activities. Actinomycetes and gram-negative bacteria were the major factors to affect soil microbial community function relating to carbon decomposition. Soil pH contributed to actinomycetes and gram-negative bacteria through direct influences on arbuscular mycorrhizal fungi. Moreover, there were strong correlations between soil pH and microbial community to control soil carbon turnover. The increasing of soil microbial abundance and the microbial regulation on soil carbon in forest thinning need to be considered for sustainable forest management practices in northern China. Full article

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is a fast-growing evergreen conifer with high-quality timber and is an important reforestation and commercial tree species in southern China. Planting density affects the productivity of Chinese fir plantations. To study the effect of five different planting densities and soil depth on soil nutrient contents of a mature C. lanceolata plantation, the soil nutrient contents (soil depths 0–100 cm) of 36-year-old mature Chinese fir plantations under five different planting densities denoted A (1667 trees·ha⁻¹), B (3333 trees·ha⁻¹), C (5000 trees·ha⁻¹), D (6667 trees·ha⁻¹), and E (10,000 trees·ha⁻¹) were measured in Pingxiang county, Guangxi province, China. Samples were collected from the soil surface down to a one meter depth from each of 45 soil profiles, and soil samples were obtained at 10 different soil depths of 0–10, 10–20, 20–30, 30–40, 40–50, 50–60, 60–70, 70–80, 80–90, and 90–100 cm. Twelve soil physical and chemical indicators were analyzed. The results showed that: (1) as planting density increased, the organic matter, organic carbon, total N and P, available N, effective Fe, and bulk density decreased. Soil pH, total K, and effective K increased with increasing planting density. Planting density did not significantly influence the exchangeable Ca and Mg. (2) Soil organic matter; organic carbon; total N and P; effective N, P, and K; exchangeable Ca and Mg; effective Fe content; and bulk density decreased with increasing soil depth. This pattern was particularly evident in the top 30 cm of the soil. (3) Excessively high planting density is not beneficial to the long-term maintenance of soil fertility in Chinese fir plantations, and the planting density of Chinese fir plantations should be maintained below 3333 stems·ha⁻¹ (density A or B) to maintain soil fertility while ensuring high yields. Full article

Decades of fire exclusion in the Southern Appalachian Mountains led to fuel accumulation and conversion from open oak-pine woodlands to closed-canopy mesic forests dominated by shade-tolerant hardwoods and shrubs that often do not support a diverse understory. Southern Appalachian forest managers and scientists recognize this and are implementing silvicultural treatments such as prescribed burning, mechanical treatments or a combination of these to restore forest structure. In this study, conducted at the Southern Appalachian Fire and Fire Surrogate Study site in Green River Game Land, North Carolina, we assessed the effects of four fuel reduction methods: burned 4x (B), mechanical treatment 2x (M), mechanical treatment 2x + burned 4x (MB), and control (C) on the changes in understory vegetation guilds from pretreatment to post-treatment years (2001–2016). The MB treatment was most effective at meeting the restoration objectives, as it resulted in increases in oak (Δ_MB = 23,400 stems/ha) and pine (Δ_MB = 900 stems/ha) stem density, importance value—calculated as the sum of relative cover and frequency—for graminoids (Δ_MB = 26.0), and density of oak stems >50 cm in height (Δ_MB = 7133 stems/ha). The B and M treatments were generally less effective, but nonetheless met a subset of the restoration objectives. The B treatment reduced ericaceous shrub cover (Δ_B = −1.2%) and increased oak stems 10–50 cm in height (Δ_B = 10,017 stems/ha), while the M treatment resulted in only modest increases of mesic hardwoods, specifically for yellow-poplar (Δ_M = 200 stems/ha) and blackgum (Δ_M = 200 stems/ha) as compared with other treatments, but significantly increased mountain laurel and rhododendron cover (Δ_M = 10.0%). Overall, these fire and fire surrogate treatments had some success in restoring understory structure, but our findings suggest a slow response in understory herbaceous vegetation. Full article

Street tree inventories are a critical component of urban forest management. However, inventories conducted in the field by trained professionals are expensive and time-consuming. Inventories relying on citizen scientists or virtual surveys conducted remotely using street-level photographs may greatly reduce the costs of street tree inventories, but there are fundamental uncertainties regarding the level of data quality that can be expected from these emerging approaches to data collection. We asked 16 volunteers to inventory street trees in suburban Chicago using Google Street View^TM imagery, and we assessed data quality by comparing their virtual survey data to field data from the same locations. We also compared virtual survey data quality according to self-rated expertise by measuring agreement within expert, intermediate, and novice analyst groups. Analyst agreement was very good for the number of trees on each street segment, and agreement was markedly lower for tree diameter class and tree identification at the genus and species levels, respectively. Interrater agreement varied by expertise, such that experts agreed with one another more often than novices for all four variables assessed. Compared to the field data, we observed substantial variability in analyst performance for diameter class estimation and tree identification, and some intermediate analysts performed as well as experts. Our findings suggest that virtual surveys may be useful for documenting the locations of street trees within a city more efficiently than field crews and with a high level of accuracy. However, tree diameter and species identification data were less reliable across all expertise groups, and especially novice analysts. Based on this analysis, virtual street tree inventories are best suited to collecting very basic information such as tree locations, or updating existing inventories to determine where trees have been planted or removed. We conclude with evidence-based recommendations for effective implementation of this type of approach. Full article

Phosphorus (P) is a necessary nutrient for plant growth and plays an important role in plant metabolisms; however, the majority of P in soil is in insoluble forms. Phosphate solubilizing bacteria (PSB) can convert the insoluble phosphates into plant-available forms and may have the potential for use in sustainable agricultural practices. This study examined the effects of two native PSB, namely Bacillus aryabhattai (JX285) and Pseudomonas auricularis (HN038), and a mixture of both strains (1:1) on the growth of Camellia oleifera Abel. seedlings. The results showed a significant promotion of the growth of C. oleifera plants by three inoculation treatments. All the PSB inoculation treatments could improve the leaf nitrogen (N) and P content and had positive effects on the available N, P, and potassium (K) content of rhizosphere soil. A co-inoculation of the two native PSB strains caused a synergistic effect and achieved the best benefit. In conclusion, B. aryabhattai and P. auricularis could be used as biological agents instead of chemical fertilizers for agricultural production to reduce environmental pollution and increase the yield of tea oil. Full article

Research Highlights: Cyclocarya paliurus, native to the subtropical region of China, is a monoecious species with a heterodichogamous mating system. Its flowering phenology and low seed success characteristics differ from other typical heterodichogamous Juglandaceae species. This could be caused by the existence of polyploidy in the population. Background and Objectives: C. paliurus has been attracting more attention as a result of its medicinal value. To meet the needs for leaf harvest, cultivation expansion is required, but this is limited by a shortage of seeds. This study aims to profile the flowering phenology and the efficacy of pollen dispersal as well as elucidate on the mechanism of low seed success in the population. Materials and Methods: The flowering phenology pattern of C. paliurus was observed in a juvenile plantation containing 835 individuals of 53 families from 8 provenances at the individual (protandry, PA and protogyny, PG) and population levels for 5 consecutive years (2014–2018). Slides with a culture medium of 10% sucrose and 0.01% boric acid were used to estimate pollen density and viability in the population, and seeds were collected from 20 randomly selected PA and PG individuals to assess seed success during 2017–2018. Results: Four flowering phenotypes and strongly skewed ratios of PA/PG and male/female occurred in the juvenile population. Sexual type and ratio changed significantly with the growth of the population over the years, showing an increasing monoecious group (11.1% to 57.2%) and a decreasing unisexual group (33.6% to 16.3%), as well as a tendency for the sexual ratio to move towards equilibrium (5.42:1 to 1.39:1 for PG:PA). Two flowering phases and bimodality in gender were displayed, as in other heterodichogamous species. However, the high overlap of inter-phases and within individuals was quite different from many previous reports. Owing to the low pollen viability of C. paliurus (~30%), low seed success was monitored in the plantation, as well as in the investigated natural populations. Conclusions: Female-bias (PG and F) and a skewed ratio of mating types corresponded to nutrient accumulation in the juvenile population. Heterodichogamy in C. paliurus was verified, but was shown to be different from other documented species in Juglandaceae. The latest finding of major tetraploidy in a natural population could explain the characteristics of the flowering phenology and seed set of C. paliurus and also give rise to more questions to be answered. Full article

The basic helix–loop–helix (bHLH) family is an important transcription factor for eukaryotes and is involved in a wide range of biological activities. Among these, bHLH can interaction with WD repeat (WD40 or WDR) and V-myb avian myeloblastosis viral oncogene homolog (MYB) form a ternary complex to promote the efficient synthesis of anthocyanins. In this study, a total of 138 jujube bHLH (ZjbHLH) family members were screened from the transcriptome of the two jujube cultivars, ‘Junzao’ (JZ) and ‘Tailihong’ (TLH). Of these, 95 ZjbHLH genes were mapped to 12 chromosomes. A phylogenetic tree was constructed using 27 arabidopsis bHLH (AtbHLH) protein sequences of Arabidopsis thaliana (L.) Heynh. and 138 ZjbHLH protein sequences of jujube. The results show that the ZjbHLH family of jujube can be divided into 12 subfamilies. The three candidate genes, ZjGL3a, ZjGL3b and ZjTT8, related to anthocyanin synthesis, were classified into subgroup III. Meanwhile, ZjGL3a, ZjGL3b and ZjTT8 have high homology with the bHLH transcription factors involved in anthocyanin synthesis in other plants. In addition, it was found that the jujube ZjbHLH transcript family showed changing patterns of expression during fruit development. The relative expression levels of ZjGL3a, ZjGL3 and ZjTT8 were consistent with the changes of the anthocyanin contents in the two jujube cultivars examined. To better understand the anthocyanin synthesis pathway involved in ZjbHLH, a regulatory pathway model for anthocyanin synthesis was constructed. This model involves the processes of anthocyanin signal transduction, synthesis and transport. Full article

In Lithuania, free-ranging European bison live sedentarily in the fragmented landscape of the central part of the country. The aim of this study was to analyze the distribution and habitat selection of European bison in a semi-isolated territory. Free-ranging European bison living sedentarily have formed six groups in the mosaic landscape of central Lithuania. A herd of 34 individuals of European bison entered into a new semi-isolated territory in 2016. During the snow-free season, we tracked the movements of the herd by fitting a global positioning system (GPS) collar to the leading cow. To evaluate the home range and habitat selection, we used ArcGIS software, Jacobs’ index, and chi-square testing to verify significant differences between proportions. The home range of European bison was largest in spring, decreased in summer, and increased again in autumn; this was associated with feeding and the food supply in agricultural lands. European bison spent more time in the forest, especially during the daytime, due to disturbance, but in summer time and at night, the bison did not avoid agricultural land. In the forests, European bison preferred clear-cut, small-leaved deciduous (aspen, grey alder) forests of middle and mature age, but they avoided broad-leaved deciduous (ash), coniferous, and young forests. The increased number of European bison caused damage to agricultural crops and increased conflict with farmers. Full article

Over the past decade, countries have strived to develop a global governance structure to halt deforestation and forest degradation, by achieving the readiness requirements for Reducing Emissions from Deforestation and forest Degradation (REDD+). Nonetheless, deforestation continues, and seemingly intact forest areas are being degraded. Furthermore, REDD+ may fail to consider the crucial ecosystem functions of forest fauna including seed dispersal and pollination. Throughout the tropics, forest animal populations are depleted by unsustainable hunting to the extent that many forests are increasingly devoid of larger mammals—a condition referred to as empty forests. Large mammals and birds, who often disperse seeds of larger more carbon-rich tree species, are preferentially targeted by hunters and the first to be depleted. Such defaunation has cascading ecosystem effects, changing forest structure and composition with implications for carbon storage capacity. Failure to address defaunation would therefore be a major oversight in REDD+, compromising its long-term viability. We carried out a desktop study reviewing REDD+ documents and national implementation efforts in Colombia, Ecuador, Nigeria, Tanzania, and Indonesia to assess the extent to which they address hunting and acknowledged the ecosystem functions of fauna. We also assessed sub-national REDD+ projects to determine whether they recognized hunting and if and how they incorporated hunting management and wildlife monitoring at the project level. Moreover, we assessed to what extent sub-national REDD+ projects addressed the long-term impacts of the sustainability of hunting on forest ecosystem function including carbon storage. We found that hunting, the risk of defaunation, and its effects have been ignored in the REDD+ policy process at both the international and national levels. At the project level, we found some reference to hunting and the risks posed by the loss of forest fauna, albeit only addressed superficially. Our results underline the fact that forest ecosystems are being reduced to their carbon content and that, despite the rhetoric of biodiversity co-benefits, fauna is not treated as a functional component of forests. This neglect threatens to undermine forest ecosystem function and service delivery as well as long-term forest carbon assimilation capacity and hence, ultimately, to compromise REDD+ objectives. Full article

The TEOSINTE BRANCHED1, CYCLOIDEA, and PROLIFERATION CELL FACTOR (TCP) transcription factor is a plant-specific gene family and acts on multiple functional genes in controlling growth, development, stress response, and the circadian clock. In this study, a class I member of the TCP family from Fraxinusmandshurica Rupr. was isolated and named FmTCP15, which encoded a protein of 362 amino acids. Protein structures were analyzed and five ligand binding sites were predicted. The phylogenetic relationship showed that FmTCP15 was most closely related to Solanaceae and Plantaginaceae. FmTCP15 was localized in the nuclei of F.mandshurica protoplast cells and highly expressed in cotyledons. The expression pattern revealed the FmTCP15 response to multiple abiotic stresses and hormone signals. Downstream genes for transient overexpression of FmTCP15 in seedlings were also investigated. A yeast two-hybrid assay confirmed that FmTCP15 could interact with DELLA proteins. FmTCP15 participated in the GA-signaling pathway, responded to abiotic stresses and hormone signals, and regulated multiple genes in these biological processes. Our study revealed the potential value of FmTCP15 for understanding the molecular mechanisms of stress and hormone signal responses. Full article