Annual Report 2022
released!
 
Article
Empagliflozin Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Rats by Modulating Sesn2/AMPK/Nrf2 Signaling and Targeting Ferroptosis and Autophagy
by , , , , , , , , , , , , , and
Int. J. Mol. Sci. 2023, 24(11), 9481; https://doi.org/10.3390/ijms24119481 (registering DOI) - 30 May 2023
Abstract
Pulmonary fibrosis (PF) is a life-threatening disorder that severely disrupts normal lung architecture and function, resulting in severe respiratory failure and death. It has no definite treatment. Empagliflozin (EMPA), a sodium-glucose cotransporter 2 (SGLT2) inhibitor, has protective potential in PF. However, the mechanisms [...] Read more.
Pulmonary fibrosis (PF) is a life-threatening disorder that severely disrupts normal lung architecture and function, resulting in severe respiratory failure and death. It has no definite treatment. Empagliflozin (EMPA), a sodium-glucose cotransporter 2 (SGLT2) inhibitor, has protective potential in PF. However, the mechanisms underlying these effects require further elucidation. Therefore, this study aimed to evaluate the ameliorative effect of EMPA against bleomycin (BLM)-induced PF and the potential mechanisms. Twenty-four male Wister rats were randomly divided into four groups: control, BLM treated, EMPA treated, and EMPA+BLM treated. EMPA significantly improved the histopathological injuries illustrated by both hematoxylin and eosin and Masson’s trichrome-stained lung tissue sections, as confirmed by electron microscopic examination. It significantly reduced the lung index, hydroxyproline content, and transforming growth factor β1 levels in the BLM rat model. It had an anti-inflammatory effect, as evidenced by a decrease in the inflammatory cytokines’ tumor necrosis factor alpha and high mobility group box 1, inflammatory cell infiltration into the bronchoalveolar lavage fluid, and the CD68 immunoreaction. Furthermore, EMPA mitigated oxidative stress, DNA fragmentation, ferroptosis, and endoplasmic reticulum stress, as evidenced by the up-regulation of nuclear factor erythroid 2-related factor expression, heme oxygenase-1 activity, glutathione peroxidase 4 levels, and a decrease in C/EBP homologous protein levels. This protective potential could be explained on the basis of autophagy induction via up-regulating lung sestrin2 expression and the LC3 II immunoreaction observed in this study. Our findings indicated that EMPA protected against BLM-induced PF-associated cellular stress by enhancing autophagy and modulating sestrin2/adenosine monophosphate-activated protein kinase/nuclear factor erythroid 2-related factor 2/heme oxygenase 1 signaling. Full article
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Communication
Dendritic Solidification and Physical Properties of Co-4.54%Sn Alloy with Broad Mushy Zone
by , and
Metals 2023, 13(6), 1046; https://doi.org/10.3390/met13061046 (registering DOI) - 30 May 2023
Abstract
The high undercooling of binary Co-4.54%Sn alloy has a significant influence on its microstructural characteristics and physical properties. Here, we report that the dendritic growth and physical properties of broad-temperature-range Co-4.54%Sn alloy remarkably depends on the undercooling during the rapid solidification. The maximum [...] Read more.
The high undercooling of binary Co-4.54%Sn alloy has a significant influence on its microstructural characteristics and physical properties. Here, we report that the dendritic growth and physical properties of broad-temperature-range Co-4.54%Sn alloy remarkably depends on the undercooling during the rapid solidification. The maximum undercooling attains 208 K at molten state, and the dendritic growth velocity is quite sluggish in highly undercooled liquid Co-4.54%Sn alloy because it has a broad solidification range of 375 K (0.21 TL); the maximum value is only 0.95 m/s at the undercooling of 175 K, which then decreases with undercooling. The microstructure refines visibly and the volume fraction of the interdendritic βCo3Sn2 phase obviously decreases with undercooling. The microhardness and electrical resistivity increase with undercooling owing to the enhancement of solute content of the primary αCo phase and refinement of the microstructure where the increased crystal boundary hinders the electronic transmission. Meanwhile, the saturation magnetization also reduces with undercooling due to the crystal particle and boundary increasing significantly, and the dendritic growth velocity and solute content increase in the primary αCo phase under rapid solidification. Full article
(This article belongs to the Topic Microstructure and Properties in Metals and Alloys)
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Article
Human Milk Oligosaccharide Profile across Lactation Stages in Israeli Women—A Prospective Observational Study
Nutrients 2023, 15(11), 2548; https://doi.org/10.3390/nu15112548 (registering DOI) - 30 May 2023
Abstract
Human milk oligosaccharides (HMOs) stimulate the growth of gut commensals, prevent the adhesion of enteropathogens and modulate host immunity. The major factors influencing variations in the HMO profile are polymorphisms in the secretor (Se) or Lewis (Le) gene, which affect the activity of [...] Read more.
Human milk oligosaccharides (HMOs) stimulate the growth of gut commensals, prevent the adhesion of enteropathogens and modulate host immunity. The major factors influencing variations in the HMO profile are polymorphisms in the secretor (Se) or Lewis (Le) gene, which affect the activity of the enzymes fucoslytransferase 2 and 3 (FUT2 and FUT3) that lead to the formation of four major fucosylated and non-fucosylated oligosaccharides (OS). This pilot study aimed to determine the HMO profile of Israeli breastfeeding mothers of 16 term and 4 preterm infants, from a single tertiary center in the Tel Aviv area. Fifty-two human milk samples were collected from 20 mothers at three-time points: colostrum, transitional milk and mature milk. The concentrations of nine HMOs were assessed using liquid chromatography coupled with mass spectra chromatograms. Fifty-five percent of the mothers were secretors and 45% were non-secretors. Infant sex affected HMO levels depending on the maternal secretor status. Secretor mothers to boys had higher levels of FUT2-dependent OS and higher levels of disialyllacto-N-tetraose in the milk of mothers to girls, whereas non-secretor mothers to girls had higher levels of 3′-sialyllactose. In addition, the season at which the human milk samples were obtained affected the levels of some HMOs, resulting in significantly lower levels in the summer. Our findings provide novel information on the irregularity in the HMO profile among Israeli lactating women and identify several factors contributing to this variability. Full article
(This article belongs to the Section Nutrition in Women)
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Article
Scientific Approaches to Solving the Problem of Joint Processes of Bubble Boiling of Refrigerant and Its Movement in a Heat Pump Heat Exchanger
by , , , , and
Energies 2023, 16(11), 4405; https://doi.org/10.3390/en16114405 (registering DOI) - 30 May 2023
Abstract
The joint processes of bubble boiling and refrigerant movement in heat pump tubes are considered. A coil located on the back side of the photovoltaic panel (PVP) is used as an additional heating surface. A mathematical model of the theoretical determination of the [...] Read more.
The joint processes of bubble boiling and refrigerant movement in heat pump tubes are considered. A coil located on the back side of the photovoltaic panel (PVP) is used as an additional heating surface. A mathematical model of the theoretical determination of the temperature on the front surface of the PVP cooled by freon in the coil from the back side has been created. A feature of the numerical simulation of bubble boiling of refrigerant in a coil was the insufficiently detailed study of the process and the lack of references to the results obtained in earlier work. The authors have clarified the boundary conditions and assumptions for numerical simulation of the bubble boiling process of refrigerant R407C. The results were compared with the theoretically found values, and later used in the design of an energy complex consisting of a heat pump and a photovoltaic panel. The mathematical model of theoretical calculation of the PVP temperature and the methodology for constructing a numerical model of bubble boiling of refrigerant as part of the methodology for designing energy technology complexes based on the authors’ plan to present a unified methodology for energy technology complexes for desalination of seawater based on other types of renewable energy sources. Full article
(This article belongs to the Topic Heat Exchanger Design and Heat Pump Efficiency)
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Review
Polymorphism of Genes and Their Impact on Beef Quality
Curr. Issues Mol. Biol. 2023, 45(6), 4749-4762; https://doi.org/10.3390/cimb45060302 (registering DOI) - 30 May 2023
Abstract
The single-nucleotide polymorphism (SNP) form of genes is a valuable source of information regarding their suitability for use as specific markers of desirable traits in beef cattle breeding. For several decades, breeding work focused on improving production efficiency through optimizing the feed conversion [...] Read more.
The single-nucleotide polymorphism (SNP) form of genes is a valuable source of information regarding their suitability for use as specific markers of desirable traits in beef cattle breeding. For several decades, breeding work focused on improving production efficiency through optimizing the feed conversion ratio and improving daily gains and meat quality. Many research teams previously undertook research work on single-nucleotide polymorphism in myostatin (MSTN), thyroglobulin (TG), calpain (CAPN), and calpastatin (CAST) proteins. The literature review focuses on the most frequently addressed issues concerning these genes in beef cattle production and points to a number of relevant studies on the genes’ polymorphic forms. The four genes presented are worth considering during breeding work as a set of genes that can positively influence productivity and production quality. Full article
(This article belongs to the Collection Feature Papers in Current Issues in Molecular Biology)
Article
Compensation-Voltage-Injection-Based Neutral-Point Voltage Fluctuation Suppression Method for NPC Converters
Energies 2023, 16(11), 4409; https://doi.org/10.3390/en16114409 (registering DOI) - 30 May 2023
Abstract
Three-level neutral-point clamped (NPC) inverters are widely used in the new energy power generation, motor drive, and many other occasions. However, the neutral-point voltage imbalance of an NPC inverter will affect the output of the inverter system, and the control of the neutral [...] Read more.
Three-level neutral-point clamped (NPC) inverters are widely used in the new energy power generation, motor drive, and many other occasions. However, the neutral-point voltage imbalance of an NPC inverter will affect the output of the inverter system, and the control of the neutral point voltage has become a popular research topic. In this paper, a compensation-voltage-injection-based neutral-point voltage balancing method is proposed. During the switching cycle, the average value model of the neutral-point current under different voltage vectors is applied to calculate the value of the compensation voltage that needs to be injected. The self-balance of the neutral-point voltage based on a switching cycle is realized, and the influence of modulation ratio and power factor on equalization ability is discussed. A MATLAB/Simulink simulation model and a small power prototype are established to verify the effectiveness of the method, and the simulation and experimental results show that the proposed method can effectively suppress the neutral-point voltage fluctuation in a wide range of modulation ratios and power factors. Full article
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Article
Understanding the Relationship between Illness Perceptions and Health Behaviour among Women with Polycystic Ovary Syndrome
Int. J. Environ. Res. Public Health 2023, 20(11), 5998; https://doi.org/10.3390/ijerph20115998 (registering DOI) - 30 May 2023
Abstract
This paper aims to delineate the cognitive, emotional, and behavioural responses of women with polycystic ovary syndrome (PCOS) to their illness by applying the Common-Sense Model of Self-Regulation (CSM) to their health behaviour. An online cross-sectional design was used to examine the relationship [...] Read more.
This paper aims to delineate the cognitive, emotional, and behavioural responses of women with polycystic ovary syndrome (PCOS) to their illness by applying the Common-Sense Model of Self-Regulation (CSM) to their health behaviour. An online cross-sectional design was used to examine the relationship between participants’ illness perceptions (illness identity, consequence, timeline, control, and cause) and emotional representations of their PCOS, and their health behaviours (diet, physical activity, and risky contraceptive behaviour). The participants were 252 women between the ages of 18 and 45 years, living in Australia, and self-reporting a diagnosis of PCOS, recruited through social media. Participants completed an online questionnaire regarding illness perceptions as well as their diet, physical activity, and risky contraceptive behaviour. Illness identity was positively associated with the number of maladaptive dietary practices (B = 0.71, 95% CI: 0.003, 0.138; p = 0.04), and perception of longer illness duration was associated with reduced physical activity (OR = 0.898, 95% CI: 0.807, 0.999; p = 0.49) and risky contraceptive behaviour (OR = 0.856, 95% CI: 0.736, 0.997; p = 0.045). The limitations of the study include all data being self-reported (including PCOS diagnosis), and the potential for analyses of physical activity and risky contraceptive use being underpowered due to reduced sample sizes. The sample was also highly educated and restricted to those who use social media. These findings suggest that illness perceptions may play a role in influencing health behaviour in women with PCOS. A better understanding of the illness perceptions of women with PCOS is needed to increase health-promoting behaviour and improve health outcomes for women with PCOS. Full article
(This article belongs to the Special Issue Polycystic Ovary Syndrome (PCOS))
Article
Multivariate Interaction Analysis of Zea mays L. Genotypes Growth Productivity in Different Environmental Conditions
Plants 2023, 12(11), 2165; https://doi.org/10.3390/plants12112165 (registering DOI) - 30 May 2023
Abstract
Evaluating maize genotypes under different conditions is important for identifying which genotypes combine stability with high yield potential. The aim of this study was to assess stability and the effect of the genotype–environment interaction (GEI) on the grain yield traits of four maize [...] Read more.
Evaluating maize genotypes under different conditions is important for identifying which genotypes combine stability with high yield potential. The aim of this study was to assess stability and the effect of the genotype–environment interaction (GEI) on the grain yield traits of four maize genotypes grown in field trials; one control trial without nitrogen, and three applying different levels of nitrogen (0, 70, 140, and 210 kg ha−1, respectively). Across two growing seasons, both the phenotypic variability and GEI for yield traits over four maize genotypes (P0725, P9889, P9757 and P9074) grown in four different fertilization treatments were studied. The additive main effects and multiplicative interaction (AMMI) models were used to estimate the GEI. The results revealed that genotype and environmental effects, such as the GEI effect, significantly influenced yield, as well as revealing that maize genotypes responded differently to different conditions and fertilization measures. An analysis of the GEI using the IPCA (interaction principal components) analysis method showed the statistical significance of the first source of variation, IPCA1. As the main component, IPCA1 explained 74.6% of GEI variation in maize yield. Genotype G3, with a mean grain yield of 10.6 t ha−1, was found to be the most stable and adaptable to all environments in both seasons, while genotype G1 was found to be unstable, following its specific adaptation to the environments. Full article
(This article belongs to the Section Crop Physiology and Crop Production)
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Article
Using Imagery Collected by an Unmanned Aerial System to Monitor Cyanobacteria in New Hampshire, USA, Lakes
Remote Sens. 2023, 15(11), 2839; https://doi.org/10.3390/rs15112839 (registering DOI) - 30 May 2023
Abstract
With the increasing occurrence of cyanobacteria blooms, it is crucial to improve our ability to monitor impacted lakes accurately, efficiently, and safely. Cyanobacteria are naturally occurring in many waters globally. Some species can release neurotoxins which cause skin irritations, gastrointestinal illness, pet/livestock fatalities, [...] Read more.
With the increasing occurrence of cyanobacteria blooms, it is crucial to improve our ability to monitor impacted lakes accurately, efficiently, and safely. Cyanobacteria are naturally occurring in many waters globally. Some species can release neurotoxins which cause skin irritations, gastrointestinal illness, pet/livestock fatalities, and possibly additional complications after long-term exposure. Using a DJI M300 RTK Unmanned Aerial Vehicle equipped with a MicaSense 10-band dual camera system, six New Hampshire lakes were monitored from May to September 2022. Using the image spectral data coupled with in situ water quality data, a random forest classification algorithm was used to predict water quality categories. The analysis yielded very high overall classification accuracies for cyanobacteria cell (93%), chlorophyll-a (87%), and phycocyanin concentrations (92%). The 475 nm wavelength, normalized green-blue difference index—version 4 (NGBDI_4), and normalized green-red difference index—version 4 (NGRDI_4) indices were the most important features for these classifications. Logarithmic regressions illuminated relationships between single bands/indices with water quality data but did not perform as well as the classification algorithm approach. Ultimately, the UAS multispectral data collected in this study successfully classified cyanobacteria cell, chlorophyll-a, and phycocyanin concentrations in the studied NH lakes. Full article
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Editorial
Safety, Tolerability, and Immunogenicity of COVID-19 Bivalent Vaccination
Vaccines 2023, 11(6), 1040; https://doi.org/10.3390/vaccines11061040 (registering DOI) - 30 May 2023
Abstract
The COVID-19 pandemic has triggered unparalleled global disruption [...] Full article
Systematic Review
Clinical Features of COVID-19 Vaccine-Associated Autoimmune Hepatitis: A Systematic Review
by and
Diseases 2023, 11(2), 80; https://doi.org/10.3390/diseases11020080 (registering DOI) - 30 May 2023
Abstract
Autoimmune hepatitis (AIH) is an inflammatory liver disease wherein the body’s immune system instigates an attack on the liver, causing inflammation and hepatic impairment. This disease usually manifests in genetically predisposed individuals and is triggered by stimuli or environments such as viral infections, [...] Read more.
Autoimmune hepatitis (AIH) is an inflammatory liver disease wherein the body’s immune system instigates an attack on the liver, causing inflammation and hepatic impairment. This disease usually manifests in genetically predisposed individuals and is triggered by stimuli or environments such as viral infections, environmental toxins, and drugs. The causal role of COVID-19 vaccination in AIH remains uncertain. This review of 39 cases of vaccine-related AIH indicates that female patients above the age of 50 years or those with potential AIH risk factors may be susceptible to vaccine-related AIH, and the clinical features of vaccine-associated AIH are similar to those of idiopathic AIH. These features commonly manifest in patients after the first dose of vaccination, with symptom onset typically delayed by 10–14 days. The incidence of underlying liver disease in patients with potential health conditions associated to liver disease is similar to that of patients without preexisting illnesses. Steroid administration is effective in treating vaccine-related AIH-susceptible patients, with most patients experiencing improvement in their clinical symptoms. However, care should be taken to prevent bacterial infections during drug administration. Furthermore, the possible pathogenic mechanisms of vaccine-associated AIH are discussed to offer potential ideas for vaccine development and enhancement. Although the incidence of vaccine-related AIH is rare, individuals should not be deterred from receiving the COVID-19 vaccine, as the benefits of vaccination significantly outweigh the risks. Full article
(This article belongs to the Topic Inflammation: The Cause of All Diseases)
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Article
Improved Extraction of High Value-Added Polyphenols from Pomegranate Peel by Solid-State Fermentation
Fermentation 2023, 9(6), 530; https://doi.org/10.3390/fermentation9060530 (registering DOI) - 30 May 2023
Abstract
Pomegranate peel is an important source of polyphenols of remarkable interest in the food, pharmaceutical and cosmetic industry. The improved extraction of total polyphenolic compounds (TPC) from pomegranate peel by solid-state fermentation (SSF) was achieved. The Box, Hunter and Hunter (BHH) followed by [...] Read more.
Pomegranate peel is an important source of polyphenols of remarkable interest in the food, pharmaceutical and cosmetic industry. The improved extraction of total polyphenolic compounds (TPC) from pomegranate peel by solid-state fermentation (SSF) was achieved. The Box, Hunter and Hunter (BHH) followed by the central composite design (CCD) processes were performed to assess the effect of the process variables on TPC release. The statistical designs indicate that the best TPC extraction (234.85 mg GAE/gdm) by means of SSF occurs at 42 °C, 50% moisture, 5.0 pH, mineral solution (g/L): NaNO3 (3.83), KH2PO4 (1.52), MgSO4 (4.66) and KCl (1.52) at 36 h. Under the best fermentation conditions TPC (248.78 ± 1.24 mgGAE/gdm) increased 5.96-fold more than values previously reported and antioxidant activity (AA) increased 5.81-fold compared to the value obtained before the SSF optimization. High-value citric acid, α and β punicalin, α and β punicalagin, punigluconin, galloyl-HHDP hexoside and ellagic acid molecules were identified. The increased extraction of TPC by SSF provides a suitable alternative for the valorization of pomegranate peel through the recovery of molecules with high added value with potential use in the food, pharmacy and cosmetic industries; a diversification in the use of food agroindustry by-products is obtained as an approach to the circular economy model through biotechnological processes. Full article
(This article belongs to the Special Issue New Trends in Solid Fermentation)
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Article
Synchronizing Many Filesystems in Near Linear Time
Future Internet 2023, 15(6), 198; https://doi.org/10.3390/fi15060198 (registering DOI) - 30 May 2023
Abstract
Finding a provably correct subquadratic synchronization algorithm for many filesystem replicas is one of the main theoretical problems in operational transformation (OT) and conflict-free replicated data types (CRDT) frameworks. Based on the algebraic theory of filesystems, which incorporates non-commutative filesystem commands natively, we [...] Read more.
Finding a provably correct subquadratic synchronization algorithm for many filesystem replicas is one of the main theoretical problems in operational transformation (OT) and conflict-free replicated data types (CRDT) frameworks. Based on the algebraic theory of filesystems, which incorporates non-commutative filesystem commands natively, we developed and built a proof-of-concept implementation of an algorithm suite which synchronizes an arbitrary number of replicas. The result is provably correct, and the synchronized system is created in linear space and time after an initial sorting phase. It works by identifying conflicting command pairs and requesting one of the commands to be removed. The method can be guided to reach any of the theoretically possible synchronized states. The algorithm also allows asynchronous usage. After the client sends a synchronization request, the local replica remains available for further modifications. When the synchronization instructions arrive, they can be merged with the changes made since the synchronization request. The suite also works on filesystems with a directed acyclic graph-based path structure in place of the traditional tree-like arrangement. Consequently, our algorithms apply to filesystems with hard or soft links as long as the links create no loops. Full article
(This article belongs to the Special Issue Software Engineering and Data Science II)
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Article
The Landscape of Telomere Length and Telomerase in Human Embryos at Blastocyst Stage
Genes 2023, 14(6), 1200; https://doi.org/10.3390/genes14061200 (registering DOI) - 30 May 2023
Abstract
The telomere length of human blastocysts exceeds that of oocytes and telomerase activity increases after zygotic activation, peaking at the blastocyst stage. Yet, it is unknown whether aneuploid human embryos at the blastocyst stage exhibit a different profile of telomere length, telomerase gene [...] Read more.
The telomere length of human blastocysts exceeds that of oocytes and telomerase activity increases after zygotic activation, peaking at the blastocyst stage. Yet, it is unknown whether aneuploid human embryos at the blastocyst stage exhibit a different profile of telomere length, telomerase gene expression, and telomerase activity compared to euploid embryos. In present study, 154 cryopreserved human blastocysts, donated by consenting patients, were thawed and assayed for telomere length, telomerase gene expression, and telomerase activity using real-time PCR (qPCR) and immunofluorescence (IF) staining. Aneuploid blastocysts showed longer telomeres, higher telomerase reverse transcriptase (TERT) mRNA expression, and lower telomerase activity compared to euploid blastocysts. The TERT protein was found in all tested embryos via IF staining with anti-hTERT antibody, regardless of ploidy status. Moreover, telomere length or telomerase gene expression did not differ in aneuploid blastocysts between chromosomal gain or loss. Our data demonstrate that telomerase is activated and telomeres are maintained in all human blastocyst stage embryos. The robust telomerase gene expression and telomere maintenance, even in aneuploid human blastocysts, may explain why extended in vitro culture alone is insufficient to cull out aneuploid embryos during in vitro fertilization. Full article
(This article belongs to the Special Issue Genetics and Genomics of Female Reproduction)
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Review
A Comprehensive Guide to Artificial Intelligence in Endoscopic Ultrasound
J. Clin. Med. 2023, 12(11), 3757; https://doi.org/10.3390/jcm12113757 (registering DOI) - 30 May 2023
Abstract
Background: Endoscopic Ultrasound (EUS) is widely used for the diagnosis of bilio-pancreatic and gastrointestinal (GI) tract diseases, for the evaluation of subepithelial lesions, and for sampling of lymph nodes and solid masses located next to the GI tract. The role of Artificial Intelligence [...] Read more.
Background: Endoscopic Ultrasound (EUS) is widely used for the diagnosis of bilio-pancreatic and gastrointestinal (GI) tract diseases, for the evaluation of subepithelial lesions, and for sampling of lymph nodes and solid masses located next to the GI tract. The role of Artificial Intelligence in healthcare in growing. This review aimed to provide an overview of the current state of AI in EUS from imaging to pathological diagnosis and training. Methods: AI algorithms can assist in lesion detection and characterization in EUS by analyzing EUS images and identifying suspicious areas that may require further clinical evaluation or biopsy sampling. Deep learning techniques, such as convolutional neural networks (CNNs), have shown great potential for tumor identification and subepithelial lesion (SEL) evaluation by extracting important features from EUS images and using them to classify or segment the images. Results: AI models with new features can increase the accuracy of diagnoses, provide faster diagnoses, identify subtle differences in disease presentation that may be missed by human eyes, and provide more information and insights into disease pathology. Conclusions: The integration of AI in EUS images and biopsies has the potential to improve the diagnostic accuracy, leading to better patient outcomes and to a reduction in repeated procedures in case of non-diagnostic biopsies. Full article
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Article
Prediction of Primary Tumor Sites in Spinal Metastases Using a ResNet-50 Convolutional Neural Network Based on MRI
Cancers 2023, 15(11), 2974; https://doi.org/10.3390/cancers15112974 (registering DOI) - 30 May 2023
Abstract
We aim to investigate the feasibility and evaluate the performance of a ResNet-50 convolutional neural network (CNN) based on magnetic resonance imaging (MRI) in predicting primary tumor sites in spinal metastases. Conventional sequences (T1-weighted, T2-weighted, and fat-suppressed T2-weighted sequences) MRIs of spinal metastases [...] Read more.
We aim to investigate the feasibility and evaluate the performance of a ResNet-50 convolutional neural network (CNN) based on magnetic resonance imaging (MRI) in predicting primary tumor sites in spinal metastases. Conventional sequences (T1-weighted, T2-weighted, and fat-suppressed T2-weighted sequences) MRIs of spinal metastases patients confirmed by pathology from August 2006 to August 2019 were retrospectively analyzed. Patients were partitioned into non-overlapping sets of 90% for training and 10% for testing. A deep learning model using ResNet-50 CNN was trained to classify primary tumor sites. Top-1 accuracy, precision, sensitivity, area under the curve for the receiver-operating characteristic (AUC-ROC), and F1 score were considered as the evaluation metrics. A total of 295 spinal metastases patients (mean age ± standard deviation, 59.9 years ± 10.9; 154 men) were evaluated. Included metastases originated from lung cancer (n = 142), kidney cancer (n = 50), mammary cancer (n = 41), thyroid cancer (n = 34), and prostate cancer (n = 28). For 5-class classification, AUC-ROC and top-1 accuracy were 0.77 and 52.97%, respectively. Additionally, AUC-ROC for different sequence subsets ranged between 0.70 (for T2-weighted) and 0.74 (for fat-suppressed T2-weighted). Our developed ResNet-50 CNN model for predicting primary tumor sites in spinal metastases at MRI has the potential to help prioritize the examinations and treatments in case of unknown primary for radiologists and oncologists. Full article
(This article belongs to the Special Issue Advances in Bone Metastatic Cancer Research)
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Article
IIR Cascaded-Resonator-Based Complex Filter Banks
Acoustics 2023, 5(2), 535-552; https://doi.org/10.3390/acoustics5020032 (registering DOI) - 30 May 2023
Abstract
The use of a filter bank of IIR filters for the spectral decomposition and analysis of signals has been popular for many years. As such, a new filter-bank resonator-based structure, representing an extremely hardware-efficient structure, has received a good deal of attention. Recently, [...] Read more.
The use of a filter bank of IIR filters for the spectral decomposition and analysis of signals has been popular for many years. As such, a new filter-bank resonator-based structure, representing an extremely hardware-efficient structure, has received a good deal of attention. Recently, multiple-resonator (MR)-based and general cascaded-resonator (CR)-based filters have been proposed. In comparison to single-resonator-based analyzers, analyzers with a higher multiplicity of resonators in the cascade provide lower side lobes and a higher attenuation in stopbands. In previous works, it was shown that the CR-based filter bank with infinite impulse response (IIR) filters, which is numerically more efficient than one with finite impulse response (FIR) filters, is suitable for dynamic harmonic analysis. This paper uses the same approach to design complex digital filter banks. In the previous case, the optimization task referred to the frequency responses of harmonic filters. In this work, the harmonic filters of the mother filter bank are reshaped so that the frequency response of the sum (or difference, depending on the parity of the number of resonators in the cascade) of two adjacent harmonic filters is optimized. This way, an online adaptive filter base can be obtained. The bandwidth of the filters in the designed filter bank can be simply changed online by adding or omitting the output signals of the corresponding harmonics of the mother filter. Full article
(This article belongs to the Special Issue Resonators in Acoustics (Volume II))
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Review
History of Mammography: Analysis of Breast Imaging Diagnostic Achievements over the Last Century
Healthcare 2023, 11(11), 1596; https://doi.org/10.3390/healthcare11111596 (registering DOI) - 30 May 2023
Abstract
Breast cancer is the most common forms of cancer and a leading cause of mortality in women. Early and correct diagnosis is, therefore, essential to save lives. The development of diagnostic imaging applied to the breast has been impressive in recent years and [...] Read more.
Breast cancer is the most common forms of cancer and a leading cause of mortality in women. Early and correct diagnosis is, therefore, essential to save lives. The development of diagnostic imaging applied to the breast has been impressive in recent years and the most used diagnostic test in the world is mammography, a low-dose X-ray technique used for imaging the breast. In the first half of the 20th century, the diagnosis was in practice only clinical, with consequent diagnostic delay and an unfavorable prognosis in the short term. The rise of organized mammography screening has led to a remarkable reduction in mortality through the early detection of breast malignancies. This historical review aims to offer a complete panorama of the development of mammography and breast imaging during the last century. Through this study, we want to understand the foundations of the pillar of radiology applied to the breast through to the most modern applications such as contrast-enhanced mammography (CEM), artificial intelligence, and radiomics. Understanding the history of the development of diagnostic imaging applied to the breast can help us understand how to better direct our efforts toward an increasingly personalized and effective diagnostic approach. The ultimate goal of imaging applied to the detection of breast malignancies should be to reduce mortality from this type of disease as much as possible. With this paper, we want to provide detailed documentation of the main steps in the evolution of breast imaging for the diagnosis of breast neoplasms; we also want to open up new scenarios where the possible current and future applications of imaging are aimed at being more precise and personalized. Full article
(This article belongs to the Special Issue Breast Cancer Prevention in Healthcare: A Comprehensive Overview)
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Review
A Review of Theoretical Knowledge and Practical Applications of Iron-Based Adsorbents for Removing Arsenic from Water
Minerals 2023, 13(6), 741; https://doi.org/10.3390/min13060741 (registering DOI) - 30 May 2023
Abstract
Groundwater contamination by arsenic (As) is a serious issue in many countries, particularly in middle- and low-income nations. High concentrations of As in drinking water and groundwater cause serious health problems. Numerous technologies, such as ion exchange, oxidation, coagulation–flocculation, phytoremediation, membrane filtration, and [...] Read more.
Groundwater contamination by arsenic (As) is a serious issue in many countries, particularly in middle- and low-income nations. High concentrations of As in drinking water and groundwater cause serious health problems. Numerous technologies, such as ion exchange, oxidation, coagulation–flocculation, phytoremediation, membrane filtration, and adsorption, have been applied to remove As from the water environment. Among these technologies, adsorption is the preferred method in low- and middle-income countries due to its affordability, high efficiency, and ease of design, operation, and maintenance. Along with this technology, a variety of materials have been developed and investigated for their ability to remove As from water environments, though iron-based adsorbents have been reported to be the most effective. Thus, the aim of this paper is to briefly review the sources of As and the prevalence of As species in water bodies, as well as the currently available technologies for As removal. A detailed analysis of recent studies on iron-based adsorbents used to remove As from aqueous solutions in both laboratory conditions and field conditions is presented. Full article
Article
Chemical Characterization, Sensory Definition and Prediction Model of the Cider Dryness from New York State Apples
Foods 2023, 12(11), 2191; https://doi.org/10.3390/foods12112191 (registering DOI) - 30 May 2023
Abstract
Cider is a fermented drink obtained from apple juice. As a function of the used apple cultivar, cider can be classified in four different categories (dry, semi-dry, semi-sweet, sweet), distinguished by the attribute of “dryness,” which reflects the sweetness and softness perceived. The [...] Read more.
Cider is a fermented drink obtained from apple juice. As a function of the used apple cultivar, cider can be classified in four different categories (dry, semi-dry, semi-sweet, sweet), distinguished by the attribute of “dryness,” which reflects the sweetness and softness perceived. The dryness level is defined by scales (IRF, NYCA scales) based on the residual sugar, titratable acidity and tannin contents. Despite some adjustments, these scales show limitations in the prediction of actual perceived dryness, as they cannot consider the complicated interrelation between combined chemical compounds and sensory perception. After defining the perceived sensory dryness and its sensory description by using the quantitative descriptive analysis (QDA) method, a multivariate approach (PLS) was applied to define a predictive model for the dryness and to identify the chemical compounds with which it was correlated. Three models were developed, based on three different sets of chemical parameters, to provide a method that is easily applicable in the ordinary production process of cider. The comparison between the predicted rating and the relative scales scores showed that the models were able to predict the dryness rating in a more effective way. The multivariate approach was found to be the most suitable to study the relation between chemical and sensory data. Full article
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Article
Predictive Role of Serum Thyroglobulin after Surgery and before Radioactive Iodine Therapy in Patients with Thyroid Carcinoma
Cancers 2023, 15(11), 2976; https://doi.org/10.3390/cancers15112976 (registering DOI) - 30 May 2023
Abstract
Introduction: Thyroidectomy followed by radioactive iodine therapy (RAI) is the treatment of choice for differentiated thyroid carcinoma (DTC). Serum thyroglobulin (Tg) measurement has proved to be useful for predicting persistent and/or recurrent disease during follow-up of DTC patients. In our study, we evaluated [...] Read more.
Introduction: Thyroidectomy followed by radioactive iodine therapy (RAI) is the treatment of choice for differentiated thyroid carcinoma (DTC). Serum thyroglobulin (Tg) measurement has proved to be useful for predicting persistent and/or recurrent disease during follow-up of DTC patients. In our study, we evaluated the risk of disease recurrence in patients with papillary thyroid carcinoma (PTC), who were treated with thyroidectomy and RAI, by measuring serum Tg at different time-points: at least 40 days after surgery, in euthyroidism with TSH < 1.5 and usually 30 days before RAI (Tg−30), on the day of RAI (Tg0), and seven days after RAI (Tg+7). Methods: One hundred and twenty-nine patients with PTC were enrolled in this retrospective study. All patients were treated with 131I for thyroid remnant ablation. Disease relapse (nodal disease or distant disease) during at least 36 months follow-up was evaluated by serum measurements of Tg, TSH, AbTg at different time points and by imaging techniques (neck ultrasonography, 131I-whole body scan (WBS) after Thyrogen® stimulation). Typically, patients were assessed at 3, 6, 12, 18, 24, and 36 months after RAI. We classified patients in five groups: (i) those who developed nodal disease (ND), (ii) those who developed distant disease (DD), (iii) those with biochemical indeterminate response and minimal residual thyroid tissue (R), (iv) those with no evidence of structural or biochemical disease + intermediate ATA risk (NED-I), and (v) those with no evidence of structural or biochemical disease + low ATA risk (NED-L). ROC curves for Tg were generated to find potential discriminating cutoffs of Tg values in all patients’ groups. Results: A total of 15 out of 129 patients (11.63%) developed nodal disease and 5 (3.88%) distant metastases, during the follow-up. We found that Tg−30 (with suppressed TSH) has the same sensitivity and specificity than Tg0 (with stimulated TSH), and it is slightly better than Tg+7, which can be influenced by the size of the residual thyroid tissue. Conclusion: Serum Tg−30 value, measured in euthyroidism 30 days before RAI, is a reliable prognostic factor to predict future nodal or distant disease, thus allowing to plan the most appropriate therapy and follow-up. Full article
(This article belongs to the Special Issue Thyroid Cancer: Translational and Clinical Studies)
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Article
Transscleral Delivery of Dexamethasone-Loaded Microparticles Using a Dissolving Microneedle Array
Pharmaceutics 2023, 15(6), 1622; https://doi.org/10.3390/pharmaceutics15061622 (registering DOI) - 30 May 2023
Abstract
Microneedles (MNs) have attracted considerable interest as a means of ocular drug delivery, a challenging delivery route due to the limitations imposed by the various biological barriers associated with this organ. In this study, a novel ocular drug delivery system was developed by [...] Read more.
Microneedles (MNs) have attracted considerable interest as a means of ocular drug delivery, a challenging delivery route due to the limitations imposed by the various biological barriers associated with this organ. In this study, a novel ocular drug delivery system was developed by formulating a dissolvable MN array containing dexamethasone-loaded PLGA microparticles for scleral drug deposition. The microparticles serve as a drug reservoir for controlled transscleral delivery. The MNs displayed sufficient mechanical strength to penetrate the porcine sclera. Dexamethasone (Dex) scleral permeation was significantly higher than in topically instilled dosage forms. The MN system was able to distribute the drug through the ocular globe, with 19.2% of the administered Dex detected in the vitreous humour. Additionally, images of the sectioned sclera confirmed the diffusion of fluorescent-labelled microparticles within the scleral matrix. The system therefore represents a potential approach for minimally invasive Dex delivery to the posterior of the eye, which lends itself to self-administration and hence high patient convenience. Full article
(This article belongs to the Special Issue 3D Printing Technology for Pharmaceutical and Biomedical Application)
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Article
β-Hydroxy-β-methyl Butyrate Regulates the Lipid Metabolism, Mitochondrial Function, and Fat Browning of Adipocytes
Nutrients 2023, 15(11), 2550; https://doi.org/10.3390/nu15112550 (registering DOI) - 30 May 2023
Abstract
A growing number of in vivo studies demonstrated that β-hydroxy-β-methyl butyrate (HMB) can serve as a lipid-lowering nutrient. Despite this interesting observation, the use of adipocytes as a model for research is yet to be explored. To ascertain the effects of HMB on [...] Read more.
A growing number of in vivo studies demonstrated that β-hydroxy-β-methyl butyrate (HMB) can serve as a lipid-lowering nutrient. Despite this interesting observation, the use of adipocytes as a model for research is yet to be explored. To ascertain the effects of HMB on the lipid metabolism of adipocytes and elucidate the underlying mechanisms, the 3T3-L1 cell line was employed. Firstly, serial doses of HMB were added to 3T3-L1 preadipocytes to evaluate the effects of HMB on cell proliferation. HMB (50 µM) significantly promoted the proliferation of preadipocytes. Next, we investigated whether HMB could attenuate fat accumulation in adipocytes. The results show that HMB treatment (50 µM) reduced the triglyceride (TG) content. Furthermore, HMB was found to inhibit lipid accumulation by suppressing the expression of lipogenic proteins (C/EBPα and PPARγ) and increasing the expression of lipolysis-related proteins (p-AMPK, p-Sirt1, HSL, and UCP3). We also determined the concentrations of several lipid metabolism-related enzymes and fatty acid composition in adipocytes. The HMB-treated cells showed reduced G6PD, LPL, and ATGL concentrations. Moreover, HMB improved the fatty acid composition in adipocytes, manifested by increases in the contents of n6 and n3 PUFAs. The enhancement of the mitochondrial respiratory function of 3T3-L1 adipocytes was confirmed via Seahorse metabolic assay, which showed that HMB treatment elevated basal mitochondrial respiration, ATP production, H+ leak, maximal respiration, and non-mitochondrial respiration. In addition, HMB enhanced fat browning of adipocytes, and this effect might be associated with the activation of the PRDM16/PGC-1α/UCP1 pathway. Taken together, HMB-induced changes in the lipid metabolism and mitochondrial function may contribute to preventing fat deposition and improving insulin sensitivity. Full article
(This article belongs to the Special Issue Nutritional Regulation of Insulin Resistance and Lipid Metabolism)
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Systematic Review
Prognosis Communication in Pediatric Oncology: A Systematic Review
Children 2023, 10(6), 972; https://doi.org/10.3390/children10060972 (registering DOI) - 30 May 2023
Abstract
Background: While communication plays an important role in medicine, it also often represents a challenge when the topic at hand is the prognosis of a high-risk condition. When it comes to pediatric oncology, the challenge becomes even greater for physicians who have to [...] Read more.
Background: While communication plays an important role in medicine, it also often represents a challenge when the topic at hand is the prognosis of a high-risk condition. When it comes to pediatric oncology, the challenge becomes even greater for physicians who have to adapt their discourse to both the child and their family. Methods: Following the PRISMA guidelines, an advanced search on PubMed, Scopus and the Cochrane Library was performed, from 1 January 2017 to 31 October 2022. Demographic data for caregivers, pediatric patients and physicians were extracted, as well as diagnosis, prognosis, presence at discussion, emotional states and impact on life, trust, decision roles, communication quality and other outcomes. Results: A total of 21 articles were analyzed. Most studies (17) focused on caregivers, while only seven and five studies were focused on children and physicians, respectively. Most parents reported high trust in their physicians (73.01%), taking the leading role in decision making (48%), moderate distress levels (46.68%), a strong desire for more information (78.64%), receiving high-quality information (56.71%) and communication (52.73%). Most children were not present at discussions (63.98%); however, their desire to know more was expressed in three studies. Moreover, only two studies observed children being involved in decision making. Most physicians had less than 20 years of experience (55.02%) and reported the use of both words and statistics (47.3%) as a communication method. Conclusions: Communication research is focused more on caregivers, yet children may understand more than they seem capable of and want to be included in the conversation. More studies should focus on and quantify the opinions of children and their physicians. In order to improve the quality of communication, healthcare workers should receive professional training. Full article
(This article belongs to the Special Issue Emerging Topic in Childhood Death Education)
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Review
Using the Microbiome as a Regenerative Medicine Strategy for Autoimmune Diseases
Biomedicines 2023, 11(6), 1582; https://doi.org/10.3390/biomedicines11061582 (registering DOI) - 30 May 2023
Abstract
Autoimmune (AI) diseases, which present in a multitude of systemic manifestations, have been connected to many underlying factors. These factors include the environment, genetics, individual microbiomes, and diet. An individual’s gut microbiota is an integral aspect of human functioning, as it is intimately [...] Read more.
Autoimmune (AI) diseases, which present in a multitude of systemic manifestations, have been connected to many underlying factors. These factors include the environment, genetics, individual microbiomes, and diet. An individual’s gut microbiota is an integral aspect of human functioning, as it is intimately integrated into the metabolic, mechanical, immunological, and neurologic pathways of the body. The microbiota dynamically changes throughout our lifetimes and is individually unique. While the gut microbiome is ever-adaptive, gut dysbiosis can exert a significant influence on physical and mental health. Gut dysbiosis is a common factor in various AI, and diets with elevated fat and sugar content have been linked to gut microbiome alterations, contributing to increased systemic inflammation. Additionally, multiple AI’s have increased levels of certain inflammatory markers such as TNF-a, IL-6, and IL-17 that have been shown to contribute to arthropathy and are also linked to increased levels of gut dysbiosis. While chronic inflammation has been shown to affect many physiologic systems, this review explores the connection between gut microbiota, bone metabolism, and the skeletal and joint destruction associated with various AI, including psoriatic arthritis, systemic lupus erythematosus, irritable bowel disease, and rheumatoid arthritis. This review aims to define the mechanisms of microbiome crosstalk between the cells of bone and cartilage, as well as to investigate the potential bidirectional connections between AI, bony and cartilaginous tissue, and the gut microbiome. By doing this, the review also introduces the concept of altering an individual’s specific gut microbiota as a form of regenerative medicine and potential tailored therapy for joint destruction seen in AI. We hope to show multiple, specific ways to target the microbiome through diet changes, rebalancing microbial diversity, or decreasing specific microbes associated with increased gut permeability, leading to reduced systemic inflammation contributing to joint pathology. Additionally, we plan to show that diet alterations can promote beneficial changes in the gut microbiota, supporting the body’s own endogenous processes to decrease inflammation and increase healing. This concept of microbial alteration falls under the definition of regenerative medicine and should be included accordingly. By implementing microbial alterations in regenerative medicine, this current study could lend increasing support to the current research on the associations of the gut microbiota, bone metabolism, and AI-related musculoskeletal pathology. Full article
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Article
Nb Phase Position Marking for Clarifying the Formation Process of Cu-Al Composite Interfacial Phases in Continuous Composite Casting
Metals 2023, 13(6), 1045; https://doi.org/10.3390/met13061045 (registering DOI) - 30 May 2023
Abstract
Cu-Al composites are widely applied materials exhibiting advanced properties of both matrix metals. Controlling the brittle interfacial phases is a key factor in improving the interfacial strength of Cu-Al composites. This paper studied the interfacial formation process of Cu-Al composites fabricated by continuous [...] Read more.
Cu-Al composites are widely applied materials exhibiting advanced properties of both matrix metals. Controlling the brittle interfacial phases is a key factor in improving the interfacial strength of Cu-Al composites. This paper studied the interfacial formation process of Cu-Al composites fabricated by continuous composite casting. The phase formation sequence, growth direction and formation mechanism were clarified via element marking and thermodynamic calculations. The spatial distribution of the interfacial phases from the aluminum side to the copper side is as follows: the α + θ layer (α-Al + CuAl2), the θ layer (CuAl2) and the γ layer (Cu9Al4). Moreover, insular η phases (CuAl) and δ phases (Cu3Al2) exist in the γ phase sublayer. The formation sequence of interfacial phases is as follows: the θ phase, the η phase, the δ phase and the γ phase. The θ layer and α + θ layer are transformed from a liquid diffusion layer formed by scouring the surface of copper with liquid aluminum, the η and δ phases grow towards the θ layer and the γ phase simultaneously grows towards both the copper matrix and the θ layer. Full article
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Article
High Performance On-Chip Energy Storage Capacitors with Plasma-Enhanced Atomic Layer-Deposited Hf0.5Zr0.5O2/Al-Doped Hf0.25Zr0.75O2 Nanofilms as Dielectrics
Nanomaterials 2023, 13(11), 1765; https://doi.org/10.3390/nano13111765 (registering DOI) - 30 May 2023
Abstract
Concurrently achieving high energy storage density (ESD) and efficiency has always been a big challenge for electrostatic energy storage capacitors. In this study, we successfully fabricate high-performance energy storage capacitors by using antiferroelectric (AFE) Al-doped Hf0.25Zr0.75O2 (HfZrO:Al) dielectrics [...] Read more.
Concurrently achieving high energy storage density (ESD) and efficiency has always been a big challenge for electrostatic energy storage capacitors. In this study, we successfully fabricate high-performance energy storage capacitors by using antiferroelectric (AFE) Al-doped Hf0.25Zr0.75O2 (HfZrO:Al) dielectrics together with an ultrathin (1 nm) Hf0.5Zr0.5O2 underlying layer. By optimizing the Al concentration in the AFE layer with the help of accurate controllability of the atomic layer deposition technique, an ultrahigh ESD of 81.4 J cm−3 and a perfect energy storage efficiency (ESE) of 82.9% are simultaneously achieved for the first time in the case of the Al/(Hf + Zr) ratio of 1/16. Meanwhile, both the ESD and ESE exhibit excellent electric field cycling endurance within 109 cycles under 5~5.5 MV cm−1, and robust thermal stability up to 200 °C. Thus, the fabricated capacitor is very promising for on-chip energy storage applications due to favorable integratability with the standard complementary metal–oxide–semiconductor (CMOS) process. Full article
(This article belongs to the Section Nanoelectronics, Nanosensors and Devices)
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