The continuous biomonitoring of a population directly or indirectly exposed to pesticides could be an additional tool for decision makers to improve their health conditions. In this work, we performed biomonitoring on two groups of people from the Mexicali Valley who were continuously exposed to pesticides using the cytokinesis-block micronucleus cytome assay (L-CBMN) to evaluate cytotoxic and genotoxic damage in human peripheral blood lymphocytes. The study groups comprised 14 indigenous Cucapah with non-vegetarian habits (NV group) from Ejido el Mayor (32.12594°, −115.27265°) and 21 lacto-ovo vegetarian (LOV) persons from the Seventh-day Adventist Church of Ejido Vicente Guerrero (32.3961°, −115.14023°). The L-CBMN assay determines the nuclear division index (NDI), apoptosis, necrosis, micronuclei (MNs), nuclear buds (NBUDs), and nucleoplasmic bridges (NPBs). Our results show that, regardless of diet or daily habits, both the studied groups presented with cytogenotoxic damage compared with non-exposed pesticide individuals, without modifications to the nuclear division index. In the rest of the evaluated biomarkers, the NV group exhibited greater cytotoxic and genotoxic damage than the LOV group. Nevertheless, individuals practicing a lacto-ovo vegetarian diet (LOV) showed lower damage than those with non-vegetarian habits (NV), suggesting a better antioxidant response that helps decrease the genotoxic damage due to the enhanced intake of folates and antioxidants from a plant-based diet. Full article

In the core networking of smart cities, mobile network operators need solutions to reflect service function chaining (SFC) orchestration policies while ensuring efficient resource utilization and preserving quality of service (QoS) in large-scale networking congestion states. To offer this solution, we observe the standardized QoS class identifiers of smart city scenarios. Then, we reflect the service criticalities via cloning virtual network function (VNF) with reserved resources for ensuring effective scheduling of request queue management. We employ graph neural networks (GNN) with a message-passing mechanism to iteratively update hidden states of VNF nodes with the objectives of enhancing allocation of resource blocks, accurate detection of availability statuses, and duplication of heavily congested instances. The deployment properties of smart city use cases are presented along with their intelligent service functions, and we aim to activate a modular architecture with multi-purpose VNFs and chaining isolation for generalizing global instances. Experimental simulation is conducted to illustrate how the proposed scheme performs under different congestion levels of SFC request rates, while capturing the key performance metrics of average delay, acceptance ratios, and completion ratios. Full article

Control-flow statements often cause misunderstandings among novice computer science students. To better address these problems, teachers need to know the misconceptions that are typical at this stage. In this paper, we present the results of studying students’ misconceptions about control-flow statements. We compiled 181 questions, each containing an algorithm written in pseudocode and the execution trace of that algorithm. Some of the traces were correct; others contained highlighted errors. The students were asked to explain in their own words why the selected line of the trace was correct or erroneous. We collected and processed 10,799 answers from 67 CS1 students. Among the 24 misconceptions we found, 6 coincided with misconceptions from other studies, and 7 were narrower cases of known misconceptions. We did not find previous research regarding 11 of the misconceptions we identified. Full article

Crystallization of bisphenol A (DGEBA)- and bisphenol F (DGEBF)-based epoxy resins is a natural property of these oligomers. However, manufacturers of coatings and other systems based on these epoxy resins are making efforts to slow down the crystallization process as much as possible, thereby extending the shelf life and improving the competitiveness of their products. This paper focuses on the kinetics of the crystallization process of epoxy resins and the effect of the presence of a certain degree of crystallinity on selected parameters of epoxy-based materials. Furthermore, an analysis of the impact of a certain degree of crystallinity of the epoxy base on the resulting coating parameters was carried out. The highest value of crystallinity (17%) was achieved in the sample containing the highest proportion of DGEBF in the crystallization phase “c”, and the enthalpy of melting (H_t) of the crystalline DGEBF sample was 6.3 J/g. Mechanical parameters as well as chemical and thermal resistance of hydrophobic epoxy systems were investigated. The best abrasion resistance (1.5 cm³/50 cm²) was achieved with the blend containing only amorphous DGEBA. The adhesion of the epoxy samples on concrete was greater than 6.5 MPa. The chemical resistance tests performed showed that, in general, the chemical resistance of epoxy systems decreases with increasing crystallinity content. The tighter arrangement of molecules in the crystalline regions of the epoxy matrix results in an increase in density, strength and hardness. This study presents a comprehensive examination of the crystallization of DGEBA and DGEBF, which is, as yet virtually unavailable. It also contributes to knowledge by outlining the possibility of speeding up or slowing down the crystallization process of epoxy resins, including the principle of selecting nucleating agents. Full article

Prescribed fire is an important land conservation tool to meet ecological, cultural, and public safety objectives across terrestrial ecosystems. While estimates of prescribed burning in the U.S.A. exceed 4.5 million hectares annually, tracking the extent of prescribed fire is problematic for several reasons and prevents an understanding of spatial and temporal trends in landscape patterns of prescribed fires. We developed a regional prescribed fire database from 12 state forestry agencies in the southeastern U.S. using records of burn location, size, and calendar days and evaluated spatial and temporal patterns in burning from 2010 to 2020. Over half of all prescribed fires in the U.S. occur in the Southeast, with five states (Florida, Georgia, Alabama, South Carolina, and Mississippi) comprising over ninety percent of the burned area over a decade. We identified hot spots of concentrated prescribed fire activity on both public and private forestlands across the region, as well as regions of less burning, which often occurred in close proximity to hot spots. Temporally, most prescribed fires occurred in March and February across the region; the least activity was recorded between May and November. Our database reveals that burning is highly concentrated within the region, presumably reflecting local land ownership categories and associated land management objectives. This database and these analyses provide the first region-wide summary of fine-scale patterns of prescribed fire in the U.S. and demonstrate the potential for various analyses beyond this work for air quality modeling and remote sensing, as well as the potential impacts of demographic and land use changes. Full article

(1) Background: In the last few years, many randomized controlled trials (RCTs) have compared direct Macintosh laryngoscopy with McGrath videolaryngoscopy in order to assess the potential benefits of the latter; the results were sometimes controversial. (2) Methods: We conducted a comprehensive literature search to identify our articles according to inclusion and exclusion criteria: to be included, each study had to be a prospective randomized trial or comparison between the McGrath videolaryngoscope and the Macintosh laryngoscope in an adult population. We did not include manikin trials or studies involving double-lumen tubes. (3) Results: 10 studies met the inclusion criteria necessary. In total, 655 patients were intubated with the McGrath and 629 with the Macintosh. In total, 1268 of 1284 patients were successfully intubated, showing equivalent results for the two devices: 648 of 655 patients with the McGrath videolaryngoscope and 620 of 629 patients with the Macintosh laryngoscope. No differences were noted in terms of hemodynamic changes or the incidence of adverse events. (4) Conclusions: We can assert that the McGrath videolaryngoscope and Macintosh laryngoscope, even if with equivalent tracheal intubation results, supplement each other. Full article

In all organizations, many decision analysts acquire their skills through the experience of facing challenges to structure complex problems. Therefore, every day, the use of tools to integrate indicators through multi-attribute ordering, component-based separation, and clustering to reduce the criteria required for decision-making and the achievement of goals and objectives is more frequent. Thus, our proposal consists of a new hybrid-hierarchical model for the classification and prediction of country indicators such as inflation, unemployment, population growth, and labor force, among others, in a decision-making environment using unsupervised neural networks and type-3 fuzzy systems. The contribution is achieving a type-3 fuzzy aggregation method in which the hierarchy is first represented by neural networks and later a set of type-1, type-2, and type-3 systems to combine the results, which allows multiple indicators to be separated and then integrated in an appropriate fashion. We can point out as one of the advantages of utilizing the method that the user can evaluate a range of qualities in multiple variables through the classification and prediction of time series attributes and assess a range of qualities for decision-making with uncertainty, according to the results of the simulations carried out. Full article

This study aimed to assess the impact of bacterial species and fermentation time on wheat bread quality, FODMAP (fermentable oligosaccharides, disaccharides, monosaccharides, and polyols) content, and antioxidant activity of wheat bread, utilizing boosted native sourdough as a novel approach to enhance bread production. The incorporation of lactic acid bacteria strains, i.e., Lacticaseibacillus casei and Lactiplantibacillus plantarum, during 72 h fermentation significantly reduced FODMAP content to less than 0.1 g/100 g of wheat bread. Extending the fermentation time to 72 h notably increased the polyphenol content to 145.35 mg gallic acid (GA) per 100 g in the case of spontaneous fermentation and to 151.11 and 198.73 mg GA/100 g in the case of sourdoughs inoculated with L. casei and L. plantarum, respectively. While the treatment yielded positive effects on FODMAP modulation and antioxidant activity, it is crucial to acknowledge its impact on some organoleptic properties, such as aroma and flavor, which, despite good overall bread quality, have changed as a result of prolonged fermentation time. The study results indicate that choosing specific bacterial species and controlling fermentation time can effectively reduce FODMAPs and boost antioxidants. These findings contribute to the understanding of sourdough-based interventions in bread production, offering insights for the development of healthier and nutritionally improved wheat bread products. Full article

This study aims to predict vital soil physical properties, including clay, sand, and silt, which are essential for agricultural management and environmental protection. Precision distribution of soil texture is crucial for effective land resource management and precision agriculture. To achieve this, we propose an innovative approach that combines Geospatial Artificial Intelligence (GeoAI) with the fusion of satellite imagery to predict soil physical properties. We collected 317 soil samples from Iran’s Golestan province for dependent data. The independent dataset encompasses 14 parameters from Landsat-8 satellite images, seven topographic parameters from the Shuttle Radar Topography Mission (SRTM) DEM, and two meteorological parameters. Using the Random Forest (RF) algorithm, we conducted feature importance analysis. We employed a Convolutional Neural Network (CNN), RF, and our hybrid CNN-RF model to predict soil properties, comparing their performance with various metrics. This hybrid CNN-RF network combines the strengths of CNN networks and the RF algorithm for improved soil texture prediction. The hybrid CNN-RF model demonstrated superior performance across metrics, excelling in predicting sand (MSE: 0.00003%, RMSE: 0.006%), silt (MSE: 0.00004%, RMSE: 0.006%), and clay (MSE: 0.00005%, RMSE: 0.007%). Moreover, the hybrid model exhibited improved precision in predicting clay (R²: 0.995), sand (R²: 0.992), and silt (R²: 0.987), as indicated by the R² index. The RF algorithm identified MRVBF, LST, and B7 as the most influential parameters for clay, sand, and silt prediction, respectively, underscoring the significance of remote sensing, topography, and climate. Our integrated GeoAI-satellite imagery approach provides valuable tools for monitoring soil degradation, optimizing agricultural irrigation, and assessing soil quality. This methodology has significant potential to advance precision agriculture and land resource management practices. Full article

Postpartum hemorrhage (PPH) remains a significant obstetric emergency worldwide and a leading cause of maternal death. However, it is commonly underreported, which can represent a major concern for maternal morbidity and mortality. This retrospective case series study analyzed patients with red blood cell transfusion (RBCt) in the postpartum period over a four-year interval at a specific center. A total of 18,674 patients delivered between January 2018 and December 2021. Patients with postpartum RBCt were classified into two groups: those with identified PPH (i-PPH) and those without (non-i-PPH). Clinical variables, delivery details, blood loss data, and treatment information were collected. Statistical analysis involved a comparison of variables between the i-PPH and non-i-PPH groups. Univariate and multivariate analyses were performed, aiming to identify significant associations between the clinical variables and a lack of PPH identification. The incidence of RBCt was 1.26% (236 cases). Patients receiving RBCt had higher rates of cesarean delivery, twin pregnancy, labor induction, and previous cesarean section. Among patients with postpartum RBCt, 34.3% lacked an identified PPH. The rarity of postpartum RBCt contrasts with the increasing rates of PPH, highlighting the importance of diagnosing PPH and postpartum anemia. A strategy of systematic quantification of blood loss during delivery could help detect PPH and anemia before adverse consequences occur. Full article

In the ongoing search for practical uses of rare-earth metal nanoparticles, cerium dioxide nanoparticles (nanoceria) have received special attention. The purpose of this research was to study the biomedical effects of nanocrystalline forms of cerium oxide obtained by different synthesis schemes and to evaluate the effect of different concentrations of nanoceria (from 10⁻² to 10⁻⁶ M) on cells involved in the regeneration of skin cell structures such as fibroblasts, mesenchymal stem cells, and keratinocytes. Two different methods of nanoceria preparation were investigated: (1) CeO-NPs-1 by precipitation from aqueous solutions of cerium (III) nitrate hexahydrate and citric acid and (2) CeO-NPs-2 by hydrolysis of ammonium hexanitratocerate (IV) under conditions of thermal autoclaving. According to the X-ray diffraction, transmission electron microscopy, and dynamic light scattering data, CeO₂-1 consists of individual particles of cerium dioxide (3–5 nm) and their aggregates with diameters of 60–130 nm. CeO₂-2 comprises small aggregates of 8–20 nm in diameter, which consist of particles of 2–3 nm in size. Cell cultures of human fibroblasts, human mesenchymal stem cells, and human keratinocytes were cocultured with different concentrations of nanoceria sols (10⁻², 10⁻³, 10⁻⁴, 10⁻⁵, and 10⁻⁶ mol/L). The metabolic activity of all cell types was investigated by MTT test after 48 and 72 h, whereas proliferative activity and cytotoxicity were determined by quantitative cell culture counting and live/dead test. A dependence of biological effects on the method of nanoceria preparation and concentration was revealed. Data were obtained with respect to the optimal concentration of sol to achieve the highest metabolic effect in the used cell cultures. Hypotheses about the mechanisms of the obtained effects and the structure of a fundamentally new medical device for accelerated healing of skin wounds were formulated. The method of nanoceria synthesis and concentration fundamentally and significantly change the biological activity of cell cultures of different types—from suppression to pronounced stimulation. The best biological activity of cell cultures was determined through cocultivation with sols of citrate nanoceria (CeO-NPs-1) at a concentration of 10⁻³–10⁻⁴ M. Full article

With the development of sharing economy, a joint distribution mode has been increasingly adopted as the preferred cooperation mode of third-party logistics enterprises to achieve the efficient, resource-saving, and profit-optimal business goals of enterprises. In the joint distribution mode, the distribution price is one of key factors that influences the operation of the joint distribution. Thus, to acquire the optimal pricing for the logistics enterprises, we establish a pricing model based on dynamic game theory for a joint distribution system including one joint distribution company and two express enterprises. In the proposed model, two dimensions of games exist simultaneously, including the game between express competitors and the game between express and distribution enterprises. The multidimensional game leads to more complex system characteristics. Through the stability analysis, we find the Nash equilibrium point and its stability conditions. Numerical simulations are conducted to investigate the complex dynamical behaviors of the game model, such as the system stability region, the bifurcation diagram, the largest Lyapunov exponent, strange attractors, etc. The simulation results indicate that different price adjustment speeds and ranges have a significant impact on the system stability and the profits of all participants in the game. The parameter adjustment control can well dominate the chaotic behaviors of the system. Enterprises should make pricing decisions based on their market positions to promote the continuous and stable development of the operation mode of the multi-agent joint sharing distribution center. Full article

Taichung is the center of the Taiwanese precision optical industry. Optics companies are modernized and automated, with most running 24 h production lines. With machines running around the clock, production lines must be assigned engineers to handle unexpected situations. The optical lens industry depends on precision technology. For fully automated production lines, each production process requires an engineer to be on call to troubleshoot production problems in real-time. However, shifts are currently scheduled manually, and the staff of each unit are responsible for scheduling the various production processes for each month. Administrative staff for each engineering department must take half a day to one day to complete the shift for a month, with results that usually do not ensure the best average workload, often leading engineers to question its fairness. Considering the manpower requirements for the actual production line shift and the fairness of balancing shifts, the scope of this study is the shift scheduling of engineering staff in the assembly line to perform different duties during a fixed cycle. The research aims to provide a solution for Glorytek to increase the efficiency of engineering shift scheduling and optimize the allocation of engineering staff. We will compare the duty allocation and efficiency of the current manual shift scheduling system with a new automated one. The results show that the efficiency of shift scheduling arrangements increased by more than 96%, and the maximum number of days of staff attendance (5 days) is less than that for manual assignment (6 days) while still satisfying the shift limits stipulated by the company. Two factors remain when implementing the proposed system. First, due to technical concerns, the internal process of the scheduling arrangement would be shifted from administrative staff to the IT department. Another concern is the inevitable investment in off-the-shelf optimization software. Full article

Explosion chambers are crucial to the technology used to prevent coal mine gas explosions. Investigating the shock wave propagation law at various coal mine tunnel cross-sections helps ensure mine safety. A self-built, highly explosive experimental setup was used to conduct empirical research on straight tubes, eight sizes of single-stage explosion chambers, and multi-stage tandem explosion chambers. Ansys Fluent numerical simulation software constructed five different tandem explosion chamber models. The wave dissipation efficiency of various types of explosion chambers was calculated, the propagation law and process of shock waves across multiple explosion chambers were examined, and the best size and type of explosion chambers were summarized to increase the wave dissipation efficiency of single-stage explosion chambers. Gun silencers inspired these models. The findings indicate that the three-stage tandem explosion chamber is the best diffusion tandem combination form, the 60° silencer-type explosion chamber is the best single-stage explosion chamber modification program, and the 500 mm × 500 mm × 200 mm explosion chamber is the best single-stage explosion chamber. Full article

Motivation for food choices is one of the most important determinant of eating behavior, because it comes from within the person. The aim of this study was to observe food choice motivations and estimate differences in demographic and health characteristics towards food choice motives in the adult population (n = 675; 54% women, ≥18 years) from urban setting. Food choice motivations were assessed using an online questionnaire validated by the EATMOT project. Using K-Means cluster analysis, participants were divided into two clusters of six motivational categories for food choices. Regarding the most and least important motivations, participants in cluster 1 chose food based on emotional motivations, and in cluster 2, they chose based on environmental and political motivations. In addition, younger and obese individuals had more pronounced emotional motivations. In conclusion, this study emphasizes the need to address emotional motivations for healthier food choices among overweight and young people. In addition, the prevalence of health motivations and growing awareness of sustainability indicate a willingness to take actions that benefit personal health and the environment. Apart from providing education, it is society’s responsibility to create an environment that promotes the implementation of acquired knowledge and changes in dietary habits. Full article

The flexibility of insect wings should be considered in the design of bionic micro flapping-wing aircraft. The honeybee is an ideal biomimetic object because its wings are small and possess a concise vein pattern. In this paper, we focus on resilin, an important flexible factor in honeybees’ forewings. Both resilin joints and resilin stripes are considered in the finite element model, and their mechanical behaviors are studied comprehensively. Resilin was found to increase the static deflections in chordwise and spanwise directions by 1.4 times and 1.9 times, respectively. In modal analysis, natural frequencies of the first bending and first torsional modes were found to be decreased significantly—especially the latter, which was reduced from 500 Hz to 217 Hz—in terms of resilin joints and stripes, closely approaching flapping frequency. As a result, the rotational angle amplitude in dynamic responses is remarkable, with an amplification ratio of about six. It was also found that resilin joints and stripes together lead to well-cambered sections and improve the stress concentrations in dynamic deformation. As resilin is widespread in insect wings, the study could help our understanding of the flexible mechanism of wing structure and inspire the development of flexible airfoils. Full article

The integration of mesoscale modeling and macroscale experimentation has emerged as a promising approach for understanding and predicting the mechanical behavior and fatigue performance of fiber-reinforced polymer composites. In this work, the mean field homogenization technique is implemented to predict the fatigue performance of the carbon-fiber-reinforced polymer composites under cyclic loading conditions. To predict the number of fatigue cycles, Modified Gerber criteria are used with the stress-based Tsai–Hill failure indicator. Fatigue strength factor (α) and creep rupture strength factor (β) are experimentally evaluated and further implemented in a computational approach to predict fatigue life cycles of the composite. The effect of composite constituents, stress ratio, and loading direction are investigated in detail against the fatigue performance of the composite. Fatigue cycles are predicted at individual matrix and fiber levels at various stress ratios of 0.2, 0.4, 0.6, and 0.8 at different loading inclinations. The experimental results are compared with the mesoscale S–N curves. Full article

Marigold (Tagetes spp.) flower petals are the most vital sources of carotenoids, especially lutein esters, for the production of natural lutein to use for food, feed, and pharmaceutical industries. Several marigold cultivars are cultivated globally; however, their lutein ester composition and contents have not been widely investigated. Considering this, this study aimed to identify and quantify prominent carotenoid esters from the flower petals of ten marigold cultivars by liquid chromatography (LC)–diode-array detection (DAD)–mass spectrometry (MS). In addition, tocopherols, phytosterols, and fatty acids were analyzed by gas chromatography (GC)–flame ionization detection (FID) and GC–MS. Furthermore, the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS^•+) and 2,2-diphenyl-1-picrylhydrazyl (DPPH^•) radical scavenging abilities of lipophilic extracts were determined. The total carotenoid contents varied significantly (p < 0. 05, Tukey HSD) among cultivars, ranging from 25.62 (cv. Alaska)–2723.11 µg/g fresh weight (cv. Superboy Orange). Among the five major lutein-diesters, (all-E)-lutein-3-O-myristate-3′-O-palmitate and lutein dipalmitate were predominant. Among the studied cultivars, α-tocopherol was recorded, ranging from 167.91 (cv. Superboy Yellow) to 338.50 µg/g FW (cv. Taishan Orange). Among phytosterols, β-sitosterol was the most prevalent phytosterol, ranging between 127.08 (cv. Superboy Yellow) and 191.99 µg/g FW (cv. Taishan Yellow). Palmitic acid (C16:0; 33.36–47.43%) was the most dominant among the fatty acids. In this study, the highest contents of lutein were recorded from cv. Superboy Orange; however, due to the substantially higher flower petal yield, the cv. Durango Red can produce the highest lutein yield of 94.45 kg/ha. These observations suggest that cv. Durango Red and cv. Superboy Orange are the ideal candidates for lutein fortification in foods and also for commercial lutein extraction. Full article

This study evaluates the nutritional value of a baked pork meat product containing lyophilized dragon fruit pulp. The selected nutritional properties of a baked pork meat product fortified with lyophilized Hylocereus undatus pulp in doses of 0.5%, 1.5%, 2.5%, and 4% were evaluated. For this assessment, changes in the basic chemical composition of the products, the content of calcium, magnesium, potassium, iron, and phosphorus, and the profile of fatty acids were considered. Additionally, characteristics typical for meat products, such as pH, water activity, oxidation-reduction potential or thiobarbituric acid reactive substances, and antioxidant properties of the product during 21 days of refrigerated storage, were assessed. The findings indicate that the use of higher doses of lyophilizate, i.e., in the amounts of 2.5% and 4%, significantly (p < 0.05) increases the nutritional value of meat products, leading to an increase in the concentration of essential minerals important for the proper functioning of the human body (calcium, magnesium, potassium, and iron). These changes occurred without affecting the basic chemical composition (except for an increase in the content of fat and carbohydrates in the sample with the addition of 4% lyophilizate). The introduction of the fortification treatment improved the fatty acid profile, resulting in an increase in the content of C14:0, C16:0, C20:0, and C20:5n3. In addition, in the variant with a 4% dosage, there was an increased content of C8:0, C10:0, C16:1n7, C18:0, C18:1n9C + C18:1n9t, and C18:2n6C + C18:2n6t, C18:3n3 (alpha), C20:1n15, and C20:1n9. In this particular variant, an increase in saturated-, monounsaturated-, and polyunsaturated fatty acids was also observed, which was associated with an increased level of TBARS in meat products. However, the increase in the dose of lyophilizate caused an increase in the antiradical effect of meat extracts. Based on the results obtained, it seems reasonable to use a plant additive in the form of lyophilized dragon fruit pulp in the amount of 4.0% in the production of pork meat products. Full article

Grass-finished beef (GFB) can provide beneficial bioactive compounds to healthy diets, including omega-3 polyunsaturated fatty acids (n-3 PUFAs), conjugated linoleic acid (CLA), and secondary bioactive compounds, such as phytochemicals. The objective of this study was to compare fatty acids (FAs), micronutrients, and phytochemicals of beef fed a biodiverse pasture (GRASS), a total mixed ration (GRAIN), or a total mixed ration with 5% grapeseed extract (GRAPE). This was a two-year study involving fifty-four Red Angus steers (n = 54). GFB contained higher levels of n-3 PUFAs, vitamin E, iron, zinc, stachydrine, hippuric acid, citric acid, and succinic acid than beef from GRAIN and GRAPE (p < 0.001 for all). No differences were observed in quantified phytochemicals between beef from GRAIN and GRAPE (p > 0.05). Random forest analysis indicated that phytochemical and FA composition of meat can predict cattle diets with a degree of certainty, especially for GFB (5.6% class error). In conclusion, these results indicate that GFB contains higher levels of potentially beneficial bioactive compounds, such as n-3 PUFAs, micronutrients, and phytochemicals, compared to grain-finished beef. Additionally, the n-6:n-3 ratio was the most crucial factor capable of separating beef based on finishing diets. Full article

The milk’s natural flora, or the starter, can preserve cheesemaking and allow for microbial competition. This investigation aimed to improve cheese safety and assess its characteristics using probiotic cell pellets (LCP) or cell-free extracts (CFS). Cheese samples were collected from different areas to investigate the current contamination situation. Six CFSs of probiotics were assessed as antifungal against toxigenic fungi using liquid and solid media and their aflatoxin reduction impact. The most effective CFS was chosen for cheese coating in nanoemulsion. Coated cheese with CFS, LCP, and LCP-CFS was assessed against control for changes in chemical composition, ripening indications, rheological properties, and microbiology. Results showed significant contamination levels in the collected samples, and toxic fungi were present. Lactobacillus rhamnosus CFS has aflatoxins reducibility in liquid media. During cheese ripening, uncoated cheese showed higher fat, protein, salt content, soluble nitrogen, total volatile fatty acids, tyrosine, and tryptophan contents than coated samples, except for LCP-coating treatment. Cheese rheology indicated that coating treatments had the lowest hardness, cohesiveness, gumminess, chewiness, and springiness compared to uncoated cheese. Uncoated cheese had the highest yeast and mold counts compared to the treated ones. The LCP-CFS-coated cheese showed no Aspergillus cells for up to 40 days. Uncoated Ras cheese recorded slightly lower flavor, body, texture, and appearance scores than coated cheeses. In conclusion, coating cheese with L. rhamnosus nanoemulsion has antifungal and antiaflatoxigenic properties, even for LCP, CFS, and CFS-LCP, which could extend cheese shelf life. Full article

An on-package colorimetric label was fabricated using Hibiscus sabdariffa L. anthocyanin as a freshness indicator because its color depends on pH. The anthocyanins were embedded within a chitosan matrix. The colorimetric labels were applied to estimate the spoilage of fish food during storage at 25 °C for 3 days. According to scanning electron microscopy results, the inclusion of the anthocyanins in chitosan matrix resulted in formation dense and uniform film. The chitosan colorimetric labels had acceptable thicknesses (78–85 µm), moisture contents (14–16%), swelling indices (84–102%), water vapor permeabilities (3.0–3.2 × 10⁻¹¹ g m/m² s Pa), tensile strengths (11.3–12.3 MPa), and elongation at breaks (14–39%). It is noteworthy that the label can distinguish fish spoilage by color turn from light brown (fresh) to grayish (spoiled) by the naked-eye, due to alterations in the pH content and formation of volatile basic nitrogen during storage. Our results indicate that all-natural color labels can be an effective method to monitor the fish spoilage during storage, which may improve food quality and sustainability. Full article

The inclusion of rubber in concrete has been suggested and used in recent research. However, the reason for the inclusion of rubber into concrete is typically the need to offset the carbon footprint of concrete and other environmental concerns. The research presented here indicates that the inclusion of rubber into concrete allows for the concrete to accept fasteners and withstand withdrawal, or pullout, of the fasteners, similar to the function of wood. We refer to this as making the concrete “nailable”, in that the concrete can be nailed together either by hand or with tools designed to be used with wood. While other methods have been used to make concrete nailable, this method is novel as no known research exists indicating that there exists a rubber concrete mix that provides similar withdrawal strength as wood. Testing indicates that the concrete can be produced at a low cost due to the inclusion of the low-cost rubber infill with reinforcement wire. The result is a reinforced concrete with an allowable load that is 13% greater than in spruce and a withdrawal force up to 25% greater than the maximum in spruce. The intended function of this material is replacement of treated lumber. The proposed rubber concrete, which is a reinforced concrete, is anticipated to have a service life of 50–100 years, while treated lumber decks in the Southeastern United States have been surveyed to have an average life of only 10 years due to environmental degradation. This leads us to conclude that if a deck were to be constructed of this nailable rubber concrete, it would last approximately five times longer in a temperate environment, such as the Southeastern United States. This improvement can be provided at a relatively low cost while providing an alternative that both prevents the use of arsenic- and copper-containing compounds used in treated lumber and provides an additional recycling method for tires. Full article

The kombucha market is diverse, and competitors constantly test new components and flavours to satisfy customers’ expectations. Replacing the original brewing base, adding flavours, or using “backslopping” influence the composition of the symbiotic starter culture of bacteria and yeast (SCOBY). Yet, deep characterisation of microbial and chemical changes in kombucha consortia in coffee and orange juice during backslopping has not been implemented. This study aimed to develop new kombucha beverages in less-conventional matrices and characterise their microbiota. We studied the chemical properties and microbial growth dynamics of lactic-acid-bacteria-tailored (LAB-tailored) kombucha culture by 16S rRNA next-generation sequencing in coffee and orange juice during a backslopping process that spanned five cycles, each lasting two to four days. The backslopping changed the culture composition and accelerated the fermentation. This study gives an overview of the pros and cons of backslopping technology for the production of kombucha-based beverages. Based on research conducted using two different media, this work provides valuable information regarding the aspects to consider when using the backslopping method to produce novel kombucha drinks, as well as identifying the main drawbacks that need to be addressed. Full article