International Journal of Molecular Sciences

12 pages, 4800 KiB

Open AccessArticle

Upregulated MicroRNA-25 Mediates the Migration of Melanoma Cells by Targeting DKK3 through the WNT/β-Catenin Pathway

by Jia Huo, Yanfei Zhang, Ruilian Li, Yuan Wang, Jiawen Wu and Dingwei Zhang^*

Department of Dermatology, the Second Hospital of Xi’an Jiaotong University, Xi’an 710004, China

Int. J. Mol. Sci. 2016, 17(11), 1124; https://doi.org/10.3390/ijms17111124 - 27 Oct 2016

Cited by 40 | Viewed by 6658

Previous research indicates that microRNA-25 (miR-25) regulates carcinogenesis and the progression of various cancers, but the role of miR-25 in melanoma remains unclear. We observed that miR-25 was significantly upregulated in melanoma cell lines and tissue samples. Downregulation of miR-25 markedly suppressed invasion [...] Read more.

Previous research indicates that microRNA-25 (miR-25) regulates carcinogenesis and the progression of various cancers, but the role of miR-25 in melanoma remains unclear. We observed that miR-25 was significantly upregulated in melanoma cell lines and tissue samples. Downregulation of miR-25 markedly suppressed invasion and proliferation of melanoma cells in vitro; however, overexpression of miR-25 markedly increased melanoma cell invasion and proliferation. Moreover, we observed Dickkopf-related protein 3 (DKK3) as a direct target of miR-25 in vitro. Upregulation of DKK3 partially attenuated the oncogenic effect of miR-25 on melanoma cells. Ectopic expression of miR-25 in melanoma cells induced β-catenin accumulation in nuclear and inhibited TCF4 (T cell factor 4) activity, as well as the expression of c-Myc and Cyclin D1. In a nude xenograft model, miR-25 upregulation significantly increased A375 melanoma growth. In summary, miR-25 is upregulated in melanoma and promotes melanoma cell proliferation and invasion, partially by targeting DKK3. These results were indicated that miR-25 may serve as a potential target for the treatment of melanoma in the future. Full article

(This article belongs to the Collection Regulation by Non-coding RNAs)

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14 pages, 2368 KiB

Open AccessArticle

by Edilene S. Soares^1,†

, Leila M. Stávale^1,†, Monique C. P. Mendonça^1,2, Andressa Coope³ and Maria Alice da Cruz-Höfling^1,2,*

¹ Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas, Campinas, SP 13083-863, Brazil

² Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, SP 13083-887, Brazil

³ Laboratory of Cell Signaling, Faculty of Medical Sciences, State University of Campinas, Campinas, SP 13083-887, Brazil

^† These authors contribute equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1462; https://doi.org/10.3390/ijms17111462 - 23 Nov 2016

Cited by 3 | Viewed by 5712

Abstract

We have previously demonstrated that Phoneutria nigriventer venom (PNV) causes blood–brain barrier (BBB) breakdown, swelling of astrocytes end-feet and fluid permeation into brain interstitium in rats. Caveolae and water channels respond to BBB alterations by co-participation in shear stress response and edema formation/resolution. [...] Read more.

We have previously demonstrated that Phoneutria nigriventer venom (PNV) causes blood–brain barrier (BBB) breakdown, swelling of astrocytes end-feet and fluid permeation into brain interstitium in rats. Caveolae and water channels respond to BBB alterations by co-participation in shear stress response and edema formation/resolution. Herein, we showed post-natal developmental-related changes of two BBB-associated transporter proteins: the endothelial caveolin-1 (Cav-1), the major scaffolding protein from caveolae frame, and the astroglial aquaporin-4 (AQP4), the main water channel protein expressed in astrocytic peri-vascular end-feet processes, in the hippocampus of rats intraperitoneally-administered PNV. Western blotting protein levels; immunohistochemistry (IHC) protein distribution in CA1, CA2, and CA3 subfields; and gene expression by Real Time-Polymerase Chain Reaction (qPCR) were assessed in post-natal Day 14 (P14) and 8–10-week-old rats over critical periods of envenomation. The intensity and duration of the toxic manifestations indicate P14 neonate rats more vulnerable to PNV than adults. Histologically, the capillaries of P14 and 8–10-week-old rats treated with PNV showed perivascular edema, while controls did not. The intensity of the toxic manifestations in P14 decreases temporally (2 > 5 > 24 h), while inversely the expression of AQP4 and Cav-1 peaked at 24 h when clinically PNV-treated animals do not differ from saline controls. IHC of AQP4 revealed that hippocampal CA1 showed the least expression at 2 h when toxic manifestation was maximal. Subfield IHC quantification revealed that in P14 rats Cav-1 peaked at 24 h when toxic manifestations were absent, whereas in 8–10-week-old rats Cav-1 peaked at 2 h when toxic signs were highest, and progressively attenuated such increases until 24 h, remaining though significantly above baseline. Considering astrocyte-endothelial physical and functional interactions, we hypothesize that age-related modulations of AQP4 and Cav-1 might be linked both to changes in functional properties of astrocytes during post-natal development and in the BBB breakdown induced by the venom of P. nigriventer. Full article

(This article belongs to the Special Issue Aquaporin)

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11 pages, 1059 KiB

Open AccessReview

Biological Properties of Tocotrienols: Evidence in Human Studies

by Puvaneswari Meganathan and Ju-Yen Fu^*

Nutrition Unit, Product Development and Advisory Services Division, Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia

Int. J. Mol. Sci. 2016, 17(11), 1682; https://doi.org/10.3390/ijms17111682 - 26 Oct 2016

Cited by 63 | Viewed by 12339

Abstract

Vitamin E has been recognized as an essential vitamin since their discovery in 1922. Although the functions of tocopherols are well established, tocotrienols have been the unsung heroes of vitamin E. Due to their structural differences, tocotrienols were reported to exert distinctive properties [...] Read more.

Vitamin E has been recognized as an essential vitamin since their discovery in 1922. Although the functions of tocopherols are well established, tocotrienols have been the unsung heroes of vitamin E. Due to their structural differences, tocotrienols were reported to exert distinctive properties compared to tocopherols. While most vegetable oils contain higher amount of tocopherols, tocotrienols were found abundantly in palm oil. Nature has made palm vitamin E to contain up to 70% of total tocotrienols, among which alpha-, gamma- and delta-tocotrienols are the major constituents. Recent advancements have shown their biological properties in conferring protection against cancer, cardiovascular diseases, neurodegeneration, oxidative stress and immune regulation. Preclinical results of these physiological functions were translated into clinical trials gaining global attention. This review will discuss in detail the evidence in human studies to date in terms of efficacy, population, disease state and bioavailability. The review will serve as a platform to pave the future direction for tocotrienols in clinical settings. Full article

(This article belongs to the Special Issue Tocopherols and Tocotrienols: Metabolism and Properties)

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13 pages, 3744 KiB

Open AccessArticle

Leaf Volatile Compounds and Associated Gene Expression during Short-Term Nitrogen Deficient Treatments in Cucumis Seedlings

by Jie Deng^1,†, Hong-Jun Yu^1,†, Yun-Yun Li¹, Xiao-Meng Zhang¹, Peng Liu¹, Qiang Li^1,* and Wei-Jie Jiang^1,2,*

¹ Key Laboratory of Horticultural Crops Genetic Improvement (Ministry of Agriculture), Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

² College of Forestry and Horticulture; Xinjiang Agricultural University, Urumqi 830052, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1713; https://doi.org/10.3390/ijms17111713 - 2 Nov 2016

Cited by 48 | Viewed by 4747

Abstract

Nitrogen (N) is an important macronutrient for plant growth and development, but the regulatory mechanism of volatile compounds in response to N deficiency is not well understood, especially in cucumber, which consumes excessive N during growth. In this study, the major volatile compounds [...] Read more.

Nitrogen (N) is an important macronutrient for plant growth and development, but the regulatory mechanism of volatile compounds in response to N deficiency is not well understood, especially in cucumber, which consumes excessive N during growth. In this study, the major volatile compounds from cucumber leaves subjected to N deficiency were analyzed by GC-MS. A total of 24 volatile components were identified including 15 aldehydes, two ketones, two alkenes, and five other volatile compounds in 9930 leaves. Principal component analysis using volatile compounds from cucumber leaves provided good separation between N-sufficient and N-deficient treatments. The main volatiles in cucumber leaves were found to be C6 and C9 aldehydes, especially (E)-2-hexanal and (E,Z)-2,6-nonadienal. (E)-2-hexanal belonged to the C6 aldehyde and was the most abundant compound, whereas (E,Z)-2,6-nonadienal was the chief component of C9 aldehydes. During N-deficient treatment, short-chain volatile content was significantly improved at 5 day, other volatiles displayed significant reduction or no significantly changes in all sampling points. Improvement of short-chain volatiles was confirmed in the six other inbred lines at 5 day after N-deficient treatments. The expression analysis of 12 cucumber LOX genes and two HPL genes revealed that CsLOX19, CsLOX20, and CsLOX22 had common up-regulated expression patterns in response to N-deficient stress in most inbred lines; meanwhile, most sample points of CsHPL1 also had significant up-regulated expression patterns. This research focused on the relationship between volatiles in cucumber and different nitrogen environments to provide valuable insight into the effect of cultivation and management of the quality of cucumber and contributes to further research on volatile metabolism in cucumber. Full article

(This article belongs to the Section Molecular Plant Sciences)

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12 pages, 3055 KiB

Open AccessArticle

Pine Defensive Monoterpene α-Pinene Influences the Feeding Behavior of Dendroctonus valens and Its Gut Bacterial Community Structure

by Letian Xu^1,2,3

, Zhanghong Shi⁴, Bo Wang⁵, Min Lu^1,*

and Jianghua Sun¹

¹ State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, China

² TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China

³ Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, China

⁴ Fujian Provincial Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China

⁵ Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun 666303, China

Int. J. Mol. Sci. 2016, 17(11), 1734; https://doi.org/10.3390/ijms17111734 - 1 Nov 2016

Cited by 38 | Viewed by 6629

Abstract

The exposure to plant defense chemicals has negative effects on insect feeding activity and modifies insect gut microbial community composition. Dendroctonus valens is a very destructive forest pest in China, and harbors a large diversity and abundance of gut microorganisms. Host pine defensive [...] Read more.

The exposure to plant defense chemicals has negative effects on insect feeding activity and modifies insect gut microbial community composition. Dendroctonus valens is a very destructive forest pest in China, and harbors a large diversity and abundance of gut microorganisms. Host pine defensive chemicals can protect the pines from attack by the holobiont. In this study, boring length of D. valens feeding on 0 mg/g α-pinene and 9 mg/g α-pinene concentration in phloem media for 6 and 48 h were recorded, and their gut bacterial communities were analyzed in parallel. Nine milligram per gram α-pinene concentration significantly inhibited boring length of D. valens and altered its gut microbial community structure after 6 h. The inhibition of boring length from 9 mg/g α-pinene in diets ceased after 48 h. No significant differences of the bacterial communities were observed between the beetles in 0 and 9 mg/g α-pinene concentration in phloem media after 48 h. Our results showed that the inhibition of the feeding behavior of D. valens and the disturbance to its gut bacterial communities in 9 mg/g α-pinene concentration in phloem media after 6 h were eliminated after 48 h. The resilience of gut bacterial community of D. valens may help the beetle catabolize pine defense chemical. Full article

(This article belongs to the Special Issue Plant-Insect Interactions)

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9 pages, 1602 KiB

Open AccessArticle

Impact of SNPs on Protein Phosphorylation Status in Rice (Oryza sativa L.)

by Shoukai Lin^1,2, Lijuan Chen¹, Huan Tao¹, Jian Huang¹, Chaoqun Xu¹, Lin Li¹, Shiwei Ma¹, Tian Tian¹, Wei Liu¹, Lichun Xue^1,*, Yufang Ai^1,* and Huaqin He^1,*

¹ College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

² College of Environmental and Biological Engineering, Putian University, Putian 351100, China

Int. J. Mol. Sci. 2016, 17(11), 1738; https://doi.org/10.3390/ijms17111738 - 11 Nov 2016

Cited by 3 | Viewed by 4887

Abstract

Single nucleotide polymorphisms (SNPs) are widely used in functional genomics and genetics research work. The high-quality sequence of rice genome has provided a genome-wide SNP and proteome resource. However, the impact of SNPs on protein phosphorylation status in rice is not fully understood. [...] Read more.

Single nucleotide polymorphisms (SNPs) are widely used in functional genomics and genetics research work. The high-quality sequence of rice genome has provided a genome-wide SNP and proteome resource. However, the impact of SNPs on protein phosphorylation status in rice is not fully understood. In this paper, we firstly updated rice SNP resource based on the new rice genome Ver. 7.0, then systematically analyzed the potential impact of Non-synonymous SNPs (nsSNPs) on the protein phosphorylation status. There were 3,897,312 SNPs in Ver. 7.0 rice genome, among which 9.9% was nsSNPs. Whilst, a total 2,508,261 phosphorylated sites were predicted in rice proteome. Interestingly, we observed that 150,197 (39.1%) nsSNPs could influence protein phosphorylation status, among which 52.2% might induce changes of protein kinase (PK) types for adjacent phosphorylation sites. We constructed a database, SNP_rice, to deposit the updated rice SNP resource and phosSNPs information. It was freely available to academic researchers at http://bioinformatics.fafu.edu.cn. As a case study, we detected five nsSNPs that potentially influenced heterotrimeric G proteins phosphorylation status in rice, indicating that genetic polymorphisms showed impact on the signal transduction by influencing the phosphorylation status of heterotrimeric G proteins. The results in this work could be a useful resource for future experimental identification and provide interesting information for better rice breeding. Full article

(This article belongs to the Section Molecular Plant Sciences)

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12 pages, 6013 KiB

Open AccessArticle

Deletion of Pr130 Interrupts Cardiac Development in Zebrafish

by Jie Yang^1,†, Zuhua Li^1,†, Xuedong Gan², Gang Zhai³, Jiajia Gao¹, Chenling Xiong¹, Xueping Qiu¹, Xuebin Wang¹, Zhan Yin^3,* and Fang Zheng^1,*

¹ Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

² Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

³ Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1746; https://doi.org/10.3390/ijms17111746 - 11 Nov 2016

Cited by 14 | Viewed by 6461

Abstract

Protein phosphatase 2 regulatory subunit B, alpha (PPP2R3A), a regulatory subunit of protein phosphatase 2A (PP2A), is a major serine/threonine phosphatase that regulates crucial function in development and growth. Previous research has implied that PPP2R3A was involved in heart failure, and [...] Read more.

Protein phosphatase 2 regulatory subunit B, alpha (PPP2R3A), a regulatory subunit of protein phosphatase 2A (PP2A), is a major serine/threonine phosphatase that regulates crucial function in development and growth. Previous research has implied that PPP2R3A was involved in heart failure, and PR130, the largest transcription of PPP2R3A, functioning in the calcium release of sarcoplasmic reticulum (SR), plays an important role in the excitation-contraction (EC) coupling. To obtain a better understanding of PR130 functions in myocardium and cardiac development, two pr130-deletion zebrafish lines were generated using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) system. Pr130-knockout zebrafish exhibited cardiac looping defects and decreased cardiac function (decreased fractional area and fractional shortening). Hematoxylin and eosin (H&E) staining demonstrated reduced cardiomyocytes. Subsequent transmission electron microscopy revealed that the bright and dark bands were narrowed and blurred, the Z- and M-lines were fogged, and the gaps between longitudinal myocardial fibers were increased. Additionally, increased apoptosis was observed in cardiomyocyte in pr130-knockout zebrafish compared to wild-type (WT). Taken together, our results suggest that pr130 is required for normal myocardium formation and efficient cardiac contractile function. Full article

(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)

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31 pages, 11195 KiB

Open AccessArticle

Integrated Computational Approach for Virtual Hit Identification against Ebola Viral Proteins VP35 and VP40

by Muhammad Usman Mirza^1,2,*,†

and Nazia Ikram²

¹ Centre for Research in Molecular Medicine (CRiMM), The University of Lahore, Defense Road, Lahore 54000, Pakistan

² Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore 54000, Pakistan

^† Current address: Department of Pharmaceutical and Pharmacological Sciences, Rega Institute for Medical Research, Medicinal Chemistry, University of Leuven, Leuven B-3000, Belgium

Int. J. Mol. Sci. 2016, 17(11), 1748; https://doi.org/10.3390/ijms17111748 - 26 Oct 2016

Cited by 48 | Viewed by 9631

Abstract

The Ebola virus (EBOV) has been recognised for nearly 40 years, with the most recent EBOV outbreak being in West Africa, where it created a humanitarian crisis. Mortalities reported up to 30 March 2016 totalled 11,307. However, up until now, EBOV drugs have [...] Read more.

The Ebola virus (EBOV) has been recognised for nearly 40 years, with the most recent EBOV outbreak being in West Africa, where it created a humanitarian crisis. Mortalities reported up to 30 March 2016 totalled 11,307. However, up until now, EBOV drugs have been far from achieving regulatory (FDA) approval. It is therefore essential to identify parent compounds that have the potential to be developed into effective drugs. Studies on Ebola viral proteins have shown that some can elicit an immunological response in mice, and these are now considered essential components of a vaccine designed to protect against Ebola haemorrhagic fever. The current study focuses on chemoinformatic approaches to identify virtual hits against Ebola viral proteins (VP35 and VP40), including protein binding site prediction, drug-likeness, pharmacokinetic and pharmacodynamic properties, metabolic site prediction, and molecular docking. Retrospective validation was performed using a database of non-active compounds, and early enrichment of EBOV actives at different false positive rates was calculated. Homology modelling and subsequent superimposition of binding site residues on other strains of EBOV were carried out to check residual conformations, and hence to confirm the efficacy of potential compounds. As a mechanism for artefactual inhibition of proteins through non-specific compounds, virtual hits were assessed for their aggregator potential compared with previously reported aggregators. These systematic studies have indicated that a few compounds may be effective inhibitors of EBOV replication and therefore might have the potential to be developed as anti-EBOV drugs after subsequent testing and validation in experiments in vivo. Full article

(This article belongs to the Special Issue Proteins and Protein-Ligand Interactions)

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11 pages, 1174 KiB

Open AccessArticle

Study on Dicyandiamide-Imprinted Polymers with Computer-Aided Design

by Dadong Liang¹, Yan Wang², Songyang Li¹, Yuqing Li¹, Miliang Zhang¹, Yang Li¹, Weishuai Tian¹, Junbo Liu^1,*, Shanshan Tang^1,*, Bo Li^3,* and Ruifa Jin⁴

¹ College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China

² Department of resources engineering, Guangxi Modern Polytechnic College, Guangxi 547000, China

³ Hebei Provincial Key Laboratory of Inorganic Nonmetallic Materials, College of Materials Science and Engineering, North China University of Science and Technology, Tangshan 063009, China

⁴ College of Chemistry and Chemical Engineering, Chifeng University, Chifeng 024000, China

Int. J. Mol. Sci. 2016, 17(11), 1750; https://doi.org/10.3390/ijms17111750 - 26 Oct 2016

Cited by 24 | Viewed by 4921

Abstract

With the aid of theoretical calculations, a series of molecularly imprinted polymers (MIPs) were designed and prepared for the recognition of dicyandiamide (DCD) via precipitation polymerization using acetonitrile as the solvent at 333 K. On the basis of the long-range correction method of [...] Read more.

With the aid of theoretical calculations, a series of molecularly imprinted polymers (MIPs) were designed and prepared for the recognition of dicyandiamide (DCD) via precipitation polymerization using acetonitrile as the solvent at 333 K. On the basis of the long-range correction method of M062X/6-31G(d,p), we simulated the bonding sites, bonding situations, binding energies, imprinted molar ratios, and the mechanisms of interaction between DCD and the functional monomers. Among acrylamide (AM), N,N’-methylenebisacrylamide (MBA), itaconic acid (IA), and methacrylic acid (MAA), MAA was confirmed as the best functional monomer, because the strongest interaction (the maximum number of hydrogen bonds and the lowest binding energy) occurs between DCD and MAA, when the optimal molar ratios for DCD to the functional monomers were used, respectively. Additionally, pentaerythritol triacrylate (PETA) was confirmed to be the best cross-linker among divinylbenzene (DVB), ethylene glycol dimethacrylate (EGDMA), trimethylolpropane trimethylacrylate (TRIM), and PETA. This is due to the facts that the weakest interaction (the highest binding energy) occurs between PETA and DCD, and the strongest interaction (the lowest binding energy) occurs between PETA and MAA. Depending on the results of theoretical calculations, a series of MIPs were prepared. Among them, the ones prepared using DCD, MAA, and PETA as the template, the functional monomer, and the cross-linker, respectively, exhibited the highest adsorption capacity for DCD. The apparent maximum absorption quantity of DCD on the MIP was 17.45 mg/g. Full article

(This article belongs to the Section Molecular Recognition)

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16 pages, 4156 KiB

Open AccessArticle

Biophysical Properties and Motility of Human Mature Dendritic Cells Deteriorated by Vascular Endothelial Growth Factor through Cytoskeleton Remodeling

by Zu-Quan Hu^1,2,3,†

, Hui Xue^1,2,3,†, Jin-Hua Long^4,†, Yun Wang^1,2,3, Yi Jia^1,2,3, Wei Qiu^1,2,3, Jing Zhou^1,2,3, Zong-Yao Wen⁵, Wei-Juan Yao^5,* and Zhu Zeng^1,2,3,*

¹ Key Laboratory of Biological and Medical Engineering, Guizhou Medical University, Guiyang 550025, China

² Engineering Research Center of Medical Biotechnology, Guizhou Medical University, Guiyang 550025, China

³ School of Biology and Engineering, Guizhou Medical University, Guiyang 550025, China

⁴ Department of Head and Neck, Affiliated Cancer Hospital, Guizhou Medical University, Guiyang 550025, China

⁵ Hemorheology Center, School of Basic Medical Sciences, Health Science Center of Peking University, Beijing 100191, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1756; https://doi.org/10.3390/ijms17111756 - 31 Oct 2016

Cited by 25 | Viewed by 5247

Abstract

Dendritic cells (DCs), the most potent antigen-presenting cells, play a central role in the initiation, regulation, and maintenance of the immune responses. Vascular endothelial growth factor (VEGF) is one of the important cytokines in the tumor microenvironment (TME) and can inhibit the differentiation [...] Read more.

Dendritic cells (DCs), the most potent antigen-presenting cells, play a central role in the initiation, regulation, and maintenance of the immune responses. Vascular endothelial growth factor (VEGF) is one of the important cytokines in the tumor microenvironment (TME) and can inhibit the differentiation and functional maturation of DCs. To elucidate the potential mechanisms of DC dysfunction induced by VEGF, the effects of VEGF on the biophysical characteristics and motility of human mature DCs (mDCs) were investigated. The results showed that VEGF had a negative influence on the biophysical properties, including electrophoretic mobility, osmotic fragility, viscoelasticity, and transmigration. Further cytoskeleton structure analysis by confocal microscope and gene expression profile analyses by gene microarray and real-time PCR indicated that the abnormal remodeling of F-actin cytoskeleton may be the main reason for the deterioration of biophysical properties, motility, and stimulatory capability of VEGF-treated mDCs. This is significant for understanding the biological behavior of DCs and the immune escape mechanism of tumors. Simultaneously, the therapeutic efficacies may be improved by blocking the signaling pathway of VEGF in an appropriate manner before the deployment of DC-based vaccinations against tumors. Full article

(This article belongs to the Section Biochemistry)

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17 pages, 15640 KiB

Open AccessArticle

Exogenous Melatonin Improves Plant Iron Deficiency Tolerance via Increased Accumulation of Polyamine-Mediated Nitric Oxide

by Cheng Zhou^1,2,†, Zhi Liu^1,†, Lin Zhu¹, Zhongyou Ma², Jianfei Wang^2,* and Jian Zhu^1,*

¹ School of Life Science and Technology, Tongji University, Shanghai 200092, China

² Key Laboratory of Bio-organic Fertilizer Creation, Ministry of Agriculture, Anhui Science and Technology University, Bengbu 233100, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1777; https://doi.org/10.3390/ijms17111777 - 25 Oct 2016

Cited by 95 | Viewed by 8249

Abstract

Melatonin has recently been demonstrated to play important roles in the regulation of plant growth, development, and abiotic and biotic stress responses. However, the possible involvement of melatonin in Fe deficiency responses and the underlying mechanisms remained elusive in Arabidopsis thaliana. In [...] Read more.

Melatonin has recently been demonstrated to play important roles in the regulation of plant growth, development, and abiotic and biotic stress responses. However, the possible involvement of melatonin in Fe deficiency responses and the underlying mechanisms remained elusive in Arabidopsis thaliana. In this study, Fe deficiency quickly induced melatonin synthesis in Arabidopsis plants. Exogenous melatonin significantly increased the soluble Fe content of shoots and roots, and decreased the levels of root cell wall Fe bound to pectin and hemicellulose, thus alleviating Fe deficiency-induced chlorosis. Intriguingly, melatonin treatments induced a significant increase of nitric oxide (NO) accumulation in roots of Fe-deficient plants, but not in those of polyamine-deficient (adc2-1 and d-arginine-treated) plants. Moreover, the melatonin-alleviated leaf chlorosis was blocked in the polyamine- and NO-deficient (nia1nia2noa1 and c-PTIO-treated) plants, and the melatonin-induced Fe remobilization was largely inhibited. In addition, the expression of some Fe acquisition-related genes, including FIT1, FRO2, and IRT1 were significantly up-regulated by melatonin treatments, whereas the enhanced expression of these genes was obviously suppressed in the polyamine- and NO-deficient plants. Collectively, our results provide evidence to support the view that melatonin can increase the tolerance of plants to Fe deficiency in a process dependent on the polyamine-induced NO production under Fe-deficient conditions. Full article

(This article belongs to the Section Molecular Plant Sciences)

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13 pages, 5434 KiB

Open AccessArticle

iTRAQ-Based Quantitative Proteomic Analysis of the Potentiated and Dormant Antler Stem Cells

by Zhen Dong^1,2, Hengxing Ba^1,2, Wei Zhang^1,2

, Dawn Coates³ and Chunyi Li^1,2,*

¹ Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China

² State Key Laboratory for Molecular Biology of Special Economic Animals, Changchun 130112, China

³ Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin 9054, New Zealand

Int. J. Mol. Sci. 2016, 17(11), 1778; https://doi.org/10.3390/ijms17111778 - 25 Oct 2016

Cited by 24 | Viewed by 6326

Abstract

As the only known organ that can completely regenerate in mammals, deer antler is of real significance in the field of regenerative medicine. Recent studies have shown that the regenerative capacity of the antlers comes from the pedicle periosteum and the cells resident [...] Read more.

As the only known organ that can completely regenerate in mammals, deer antler is of real significance in the field of regenerative medicine. Recent studies have shown that the regenerative capacity of the antlers comes from the pedicle periosteum and the cells resident in the periosteum possess the attributes of stem cells. Currently, the molecular mechanism of antler regeneration remains unclear. In the present study, we compared the potentiated and dormant antler stem cells using isobaric tags for the relative and absolute quantification (iTRAQ) labeling of the peptides, coupled with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) to compare the proteome profiles. Proteins were identified by searching against the NCBI nr database and our own Cervine transcriptome database, and bioinformatics analysis was conducted to identify the differentially expressed proteins. Based on this searching strategy, we identified 169 differentially expressed proteins in total, consisting of 70 up- and 99 down-regulated in the potentiated vs. dormant antler stem cells. Reliability of the iTRAQ was confirmed via quantitative real-time polymerase chain reaction (qRT-PCR) to measure the expression of selected genes. We identified transduction pathways through the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, such as HIF-1 and PI3K-AKT signaling pathways that play important roles in regulating the regeneration of antlers. In summary, the initiation stage of antler regeneration, a process from dormant to potentiated states in antler stem cells, is regulated by multiple proteins and complicated signal networks. Full article

(This article belongs to the Special Issue Advances in Proteomic Research)

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15 pages, 3010 KiB

Open AccessArticle

Linear Response Function of Bond-Order

by Nayuta Suzuki, Yuki Mitsuta, Mitsutaka Okumura and Shusuke Yamanaka^*

Graduate School of Science, Osaka University, Machikaneyama 1-1, Toyoanaka, Osaka 560-0043, Japan

Int. J. Mol. Sci. 2016, 17(11), 1779; https://doi.org/10.3390/ijms17111779 - 25 Oct 2016

Cited by 1 | Viewed by 5204

Abstract

We present the linear response function of bond-orders (LRF-BO) based on a real space integration scheme for molecular systems. As in the case of the LRF of density, the LRF-BO is defined as the response of the bond order of the molecule for [...] Read more.

We present the linear response function of bond-orders (LRF-BO) based on a real space integration scheme for molecular systems. As in the case of the LRF of density, the LRF-BO is defined as the response of the bond order of the molecule for the virtual perturbation. Our calculations show that the LRF-BO enables us not only to detect inductive and resonating effects of conjugating systems, but also to predict pK_a values on substitution groups via linear relationships between the Hammett constants and the LRF-BO values for meta- and para-substituted benzoic acids. More importantly, the LRF-BO values for the O-H bonds strongly depend on the sites to which the virtual perturbation is applied, implying that the LRF-BO values include essential information about reaction mechanism of the acid-dissociation of substituted benzoic acids. Full article

(This article belongs to the Special Issue Chemical Bond and Bonding 2016)

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17 pages, 12599 KiB

Open AccessArticle

Icariin Promotes Tendon-Bone Healing during Repair of Rotator Cuff Tears: A Biomechanical and Histological Study

by Chenyi Ye^1,2,†, Wei Zhang^1,2,†, Shengdong Wang^1,2,†, Shuai Jiang^1,2,3, Yuanbin Yu^1,2, Erman Chen^1,2, Deting Xue^1,2, Jianzhong Chen⁴ and Rongxin He^1,2,*

¹ Department of Orthopedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, No. 88, Jiefang Road, Hangzhou 310009, China

² Orthopedics Research Institute of Zhejiang University, No. 88, Jiefang Road, Hangzhou 310009, China

³ Department of Hand Surgery, the First Affiliated Hospital, School of Medicine, Zhejiang University, No. 79 Qingchun Road, Hangzhou 310009, China

⁴ Institute of Immunology, School of Basic Medical Sciences, Zhejiang University, No. 866, Yuhangtang Road, Hangzhou 310000, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1780; https://doi.org/10.3390/ijms17111780 - 25 Oct 2016

Cited by 21 | Viewed by 7361

Abstract

To investigate whether the systematic administration of icariin (ICA) promotes tendon-bone healing after rotator cuff reconstruction in vivo, a total of 64 male Sprague Dawley rats were used in a rotator cuff injury model and underwent rotator cuff reconstruction (bone tunnel suture fixation). [...] Read more.

To investigate whether the systematic administration of icariin (ICA) promotes tendon-bone healing after rotator cuff reconstruction in vivo, a total of 64 male Sprague Dawley rats were used in a rotator cuff injury model and underwent rotator cuff reconstruction (bone tunnel suture fixation). Rats from the ICA group (n = 32) were gavage-fed daily with ICA at 0.125 mg/g, while rats in the control group (n = 32) received saline only. Micro-computed tomography, biomechanical tests, serum ELISA (calcium; Ca, alkaline phosphatase; AP, osteocalcin; OCN) and histological examinations (Safranin O and Fast Green staining, type I, II and III collagen (Col1, Col2, and Col3), CD31, and vascular endothelial growth factor (VEGF)) were analyzed two and four weeks after surgery. In the ICA group, the serum levels of AP and OCN were higher than in the control group. More Col1-, Col2-, CD31-, and VEGF-positive cells, together with a greater degree of osteogenesis, were detected in the ICA group compared with the control group. During mechanical testing, the ICA group showed a significantly higher ultimate failure load than the control group at both two and four weeks. Our results indicate that the systematic administration of ICA could promote angiogenesis and tendon-bone healing after rotator cuff reconstruction, with superior mechanical strength compared with the controls. Treatment for rotator cuff injury using systematically-administered ICA could be a promising strategy. Full article

(This article belongs to the Special Issue Translational Molecular Medicine & Molecular Drug Discovery)

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38 pages, 15009 KiB

Open AccessReview

Carotenoid Cleavage Oxygenases from Microbes and Photosynthetic Organisms: Features and Functions

by Oussama Ahrazem¹

, Lourdes Gómez-Gómez¹

, María J. Rodrigo², Javier Avalos³

and María Carmen Limón^3,*

¹ Instituto Botánico, Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Universidad de Castilla-La Mancha, Campus Universitario s/n, 02071 Albacete, Spain

² Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Departamento de Ciencia de los Alimentos, Calle Catedrático Agustín Escardino 7, 46980 Paterna, Spain

³ Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, Spain

Int. J. Mol. Sci. 2016, 17(11), 1781; https://doi.org/10.3390/ijms17111781 - 26 Oct 2016

Cited by 152 | Viewed by 10800

Abstract

Apocarotenoids are carotenoid-derived compounds widespread in all major taxonomic groups, where they play important roles in different physiological processes. In addition, apocarotenoids include compounds with high economic value in food and cosmetics industries. Apocarotenoid biosynthesis starts with the action of carotenoid cleavage dioxygenases [...] Read more.

Apocarotenoids are carotenoid-derived compounds widespread in all major taxonomic groups, where they play important roles in different physiological processes. In addition, apocarotenoids include compounds with high economic value in food and cosmetics industries. Apocarotenoid biosynthesis starts with the action of carotenoid cleavage dioxygenases (CCDs), a family of non-heme iron enzymes that catalyze the oxidative cleavage of carbon–carbon double bonds in carotenoid backbones through a similar molecular mechanism, generating aldehyde or ketone groups in the cleaving ends. From the identification of the first CCD enzyme in plants, an increasing number of CCDs have been identified in many other species, including microorganisms, proving to be a ubiquitously distributed and evolutionarily conserved enzymatic family. This review focuses on CCDs from plants, algae, fungi, and bacteria, describing recent progress in their functions and regulatory mechanisms in relation to the different roles played by the apocarotenoids in these organisms. Full article

(This article belongs to the Section Bioactives and Nutraceuticals)

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18 pages, 4001 KiB

Open AccessArticle

Non-SMC Element 2 (NSMCE2) of the SMC5/6 Complex Helps to Resolve Topological Stress

by Dideke E. Verver^1,†, Yi Zheng^1,†, Dave Speijer², Ron Hoebe³, Henk L. Dekker⁴, Sjoerd Repping¹, Jan Stap³ and Geert Hamer^1,*

¹ Center for Reproductive Medicine, Amsterdam Research Institute Reproduction and Development, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands

² Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands

³ Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands

⁴ Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, 1090 GE Amsterdam, The Netherlands

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1782; https://doi.org/10.3390/ijms17111782 - 26 Oct 2016

Cited by 24 | Viewed by 7729

Abstract

The structural maintenance of chromosomes (SMC) protein complexes shape and regulate the structure and dynamics of chromatin, thereby controlling many chromosome-based processes such as cell cycle progression, differentiation, gene transcription and DNA repair. The SMC5/6 complex is previously described to promote DNA double-strand [...] Read more.

The structural maintenance of chromosomes (SMC) protein complexes shape and regulate the structure and dynamics of chromatin, thereby controlling many chromosome-based processes such as cell cycle progression, differentiation, gene transcription and DNA repair. The SMC5/6 complex is previously described to promote DNA double-strand breaks (DSBs) repair by sister chromatid recombination, and found to be essential for resolving recombination intermediates during meiotic recombination. Moreover, in budding yeast, SMC5/6 provides structural organization and topological stress relief during replication in mitotically dividing cells. Despite the essential nature of the SMC5/6 complex, the versatile mechanisms by which SMC5/6 functions and its molecular regulation in mammalian cells remain poorly understood. By using a human osteosarcoma cell line (U2OS), we show that after the CRISPR-Cas9-mediated removal of the SMC5/6 subunit NSMCE2, treatment with the topoisomerase II inhibitor etoposide triggered an increased sensitivity in cells lacking NSMCE2. In contrast, NSMCE2 appeared not essential for a proper DNA damage response or cell survival after DSB induction by ionizing irradiation (IR). Interestingly, by way of immunoprecipitations (IPs) and mass spectrometry, we found that the SMC5/6 complex physically interacts with the DNA topoisomerase II α (TOP2A). We therefore propose that the SMC5/6 complex functions in resolving TOP2A-mediated DSB-repair intermediates generated during replication. Full article

(This article belongs to the Section Biochemistry)

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16 pages, 1663 KiB

Open AccessReview

The Control of Calcium Metabolism in Zebrafish (Danio rerio)

by Chia-Hao Lin¹ and Pung-Pung Hwang^2,*

¹ National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan

² Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 11529, Taiwan

Int. J. Mol. Sci. 2016, 17(11), 1783; https://doi.org/10.3390/ijms17111783 - 26 Oct 2016

Cited by 60 | Viewed by 8415

Abstract

Zebrafish is an emerging model for the research of body fluid ionic homeostasis. In this review, we focus on current progress on the regulation of Ca²⁺ uptake in the context of Ca²⁺ sensing and hormonal regulation in zebrafish. Na⁺-K [...] Read more.

Zebrafish is an emerging model for the research of body fluid ionic homeostasis. In this review, we focus on current progress on the regulation of Ca²⁺ uptake in the context of Ca²⁺ sensing and hormonal regulation in zebrafish. Na⁺-K⁺-ATPase-rich cells (NaRCs), the specialized ionocytes in the embryonic skin and adult gills, play a dominant role in Ca²⁺ uptake in zebrafish. Transepithelial Ca²⁺ transport in NaRC, through apical epithelial Ca²⁺ channels (ECaC), basolateral plasma membrane Ca²⁺-ATPase (PMCA), and Na⁺/Ca²⁺ exchanger (NCX), is analogous to mammalian renal and intestinal Ca²⁺-absorption cells. Several hormones were demonstrated to differentially regulate Ca²⁺ uptake through modulating the expression of Ca²⁺ transporters and/or the proliferation/differentiation of NaRC in zebrafish. In addition, the counterbalance among these hormones is associated with the maintenance of body fluid Ca²⁺ homeostasis. Calcium-sensing receptor (CaSR) is expressed in several hormone-secreting tissues in zebrafish, and activated CaSR differentially controls calciotropic hormones. The major principles of Ca²⁺ transport and the hormonal control appear to be conserved from zebrafish to other vertebrates including mammals. The new knowledge gained from zebrafish studies provides new insights into the related issues in vertebrates. Full article

(This article belongs to the Special Issue Calcium Regulation and Sensing)

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22 pages, 1971 KiB

Open AccessReview

Prostate Cancer Detection and Prognosis: From Prostate Specific Antigen (PSA) to Exosomal Biomarkers

by Xavier Filella^* and Laura Foj

Department of Biochemistry and Molecular Genetics (CDB), Hospital Clínic, IDIBAPS, C/Villarroel, 170, 08036 Barcelona, Catalonia, Spain

Int. J. Mol. Sci. 2016, 17(11), 1784; https://doi.org/10.3390/ijms17111784 - 26 Oct 2016

Cited by 106 | Viewed by 17364

Abstract

Prostate specific antigen (PSA) remains the most used biomarker in the management of early prostate cancer (PCa), in spite of the problems related to false positive results and overdiagnosis. New biomarkers have been proposed in recent years with the aim of increasing specificity [...] Read more.

Prostate specific antigen (PSA) remains the most used biomarker in the management of early prostate cancer (PCa), in spite of the problems related to false positive results and overdiagnosis. New biomarkers have been proposed in recent years with the aim of increasing specificity and distinguishing aggressive from non-aggressive PCa. The emerging role of the prostate health index and the 4Kscore is reviewed in this article. Both are blood-based tests related to the aggressiveness of the tumor, which provide the risk of suffering PCa and avoiding negative biopsies. Furthermore, the use of urine has emerged as a non-invasive way to identify new biomarkers in recent years, including the PCA3 and TMPRSS2:ERG fusion gene. Available results about the PCA3 score showed its usefulness to decide the repetition of biopsy in patients with a previous negative result, although its relationship with the aggressiveness of the tumor is controversial. More recently, aberrant microRNA expression in PCa has been reported by different authors. Preliminary results suggest the utility of circulating and urinary microRNAs in the detection and prognosis of PCa. Although several of these new biomarkers have been recommended by different guidelines, large prospective and comparative studies are necessary to establish their value in PCa detection and prognosis. Full article

(This article belongs to the Special Issue Diagnostic, Prognostic and Predictive Biomarkers in Prostate Cancer)

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18 pages, 1124 KiB

Open AccessReview

The Impact of Vitamin E and Other Fat-Soluble Vitamins on Alzheimer´s Disease

by Marcus O. W. Grimm^1,2,3,*,†

, Janine Mett^1,† and Tobias Hartmann^1,2,3

¹ Experimental Neurology, Saarland University, Kirrberger Str. 1, 66421 Homburg/Saar, Germany

² Neurodegeneration and Neurobiology, Saarland University, Kirrberger Str. 1, 66421 Homburg/Saar, Germany

³ Deutsches Institut für DemenzPrävention (DIDP), Saarland University, Kirrberger Str. 1, 66421 Homburg/Saar, Germany

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1785; https://doi.org/10.3390/ijms17111785 - 26 Oct 2016

Cited by 89 | Viewed by 12690

Abstract

Alzheimer’s disease (AD) is the most common cause of dementia in the elderly population, currently affecting 46 million people worldwide. Histopathologically, the disease is characterized by the occurrence of extracellular amyloid plaques composed of aggregated amyloid-β (Aβ) peptides and intracellular neurofibrillary tangles containing [...] Read more.

Alzheimer’s disease (AD) is the most common cause of dementia in the elderly population, currently affecting 46 million people worldwide. Histopathologically, the disease is characterized by the occurrence of extracellular amyloid plaques composed of aggregated amyloid-β (Aβ) peptides and intracellular neurofibrillary tangles containing the microtubule-associated protein tau. Aβ peptides are derived from the sequential processing of the amyloid precursor protein (APP) by enzymes called secretases, which are strongly influenced by the lipid environment. Several vitamins have been reported to be reduced in the plasma/serum of AD-affected individuals indicating they have an impact on AD pathogenesis. In this review we focus on vitamin E and the other lipophilic vitamins A, D, and K, and summarize the current knowledge about their status in AD patients, their impact on cognitive functions and AD risk, as well as their influence on the molecular mechanisms of AD. The vitamins might affect the generation and clearance of Aβ both by direct effects and indirectly by altering the cellular lipid homeostasis. Additionally, vitamins A, D, E, and K are reported to influence further mechanisms discussed to be involved in AD pathogenesis, e.g., Aβ-aggregation, Aβ-induced neurotoxicity, oxidative stress, and inflammatory processes, as summarized in this article. Full article

(This article belongs to the Special Issue Tocopherols and Tocotrienols: Metabolism and Properties)

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10 pages, 1688 KiB

Open AccessArticle

Circadian Rhythm Shapes the Gut Microbiota Affecting Host Radiosensitivity

by Ming Cui^1,*,†, Huiwen Xiao^1,†, Dan Luo¹, Xin Zhang¹, Shuyi Zhao¹, Qisheng Zheng¹, Yuan Li¹, Yu Zhao¹, Jiali Dong¹, Hang Li¹, Haichao Wang² and Saijun Fan^1,*

¹ Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China

² Department of Emergency Medicine, North Shore University Hospital; Laboratory of Emergency Medicine, the Feinstein Institute for Medical Research, 350 Community Drive, Manhasset, NY 11030, USA

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1786; https://doi.org/10.3390/ijms17111786 - 26 Oct 2016

Cited by 69 | Viewed by 11042

Abstract

Modern lifestyles, such as shift work, nocturnal social activities, and jet lag, disturb the circadian rhythm. The interaction between mammals and the co-evolved intestinal microbiota modulates host physiopathological processes. Radiotherapy is a cornerstone of modern management of malignancies; however, it was previously unknown [...] Read more.

Modern lifestyles, such as shift work, nocturnal social activities, and jet lag, disturb the circadian rhythm. The interaction between mammals and the co-evolved intestinal microbiota modulates host physiopathological processes. Radiotherapy is a cornerstone of modern management of malignancies; however, it was previously unknown whether circadian rhythm disorder impairs prognosis after radiotherapy. To investigate the effect of circadian rhythm on radiotherapy, C57BL/6 mice were housed in different dark/light cycles, and their intestinal bacterial compositions were compared using high throughput sequencing. The survival rate, body weight, and food intake of mice in diverse cohorts were measured following irradiation exposure. Finally, the enteric bacterial composition of irradiated mice that experienced different dark/light cycles was assessed using 16S RNA sequencing. Intriguingly, mice housed in aberrant light cycles harbored a reduction of observed intestinal bacterial species and shifts of gut bacterial composition compared with those of the mice kept under 12 h dark/12 h light cycles, resulting in a decrease of host radioresistance. Moreover, the alteration of enteric bacterial composition of mice in different groups was dissimilar. Our findings provide novel insights into the effects of biological clocks on the gut bacterial composition, and underpin that the circadian rhythm influences the prognosis of patients after radiotherapy in a preclinical setting. Full article

(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Circadian Rhythms)

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24 pages, 5652 KiB

Open AccessArticle

Cytotoxicity of 11-epi-Sinulariolide Acetate Isolated from Cultured Soft Corals on HA22T Cells through the Endoplasmic Reticulum Stress Pathway and Mitochondrial Dysfunction

by Jen-Jie Lin¹, Robert Y. L. Wang^2,3

, Jiing-Chuan Chen⁴, Chien-Chih Chiu⁵

, Ming-Hui Liao^1,*,† and Yu-Jen Wu^4,6,*,†

¹ Graduate Institute of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan

² Department of Biomedical Sciences and Molecular Medicine Research Center, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan

³ Division of Pediatric Infectious Disease, Department of Pediatrics, Chang Gung Memorial Hospital, Linkuo 33305, Taiwan

⁴ Department of Food Science and Nutrition, Meiho University, Pingtung 91202, Taiwan

⁵ Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

⁶ Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1787; https://doi.org/10.3390/ijms17111787 - 27 Oct 2016

Cited by 16 | Viewed by 5988

Abstract

Natural compounds from soft corals have been increasingly used for their antitumor therapeutic properties. This study examined 11-epi-sinulariolide acetate (11-epi-SA), an active compound isolated from the cultured soft coral Sinularia flexibilis, to determine its potential antitumor effect on [...] Read more.

Natural compounds from soft corals have been increasingly used for their antitumor therapeutic properties. This study examined 11-epi-sinulariolide acetate (11-epi-SA), an active compound isolated from the cultured soft coral Sinularia flexibilis, to determine its potential antitumor effect on four hepatocellular carcinoma cell lines. Cell viability was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the results demonstrated that 11-epi-SA treatment showed more cytotoxic effect toward HA22T cells. Protein profiling of the 11-epi-SA-treated HA22T cells revealed substantial protein alterations associated with stress response and protein synthesis and folding, suggesting that the mitochondria and endoplasmic reticulum (ER) play roles in 11-epi-SA-initiated apoptosis. Moreover, 11-epi-SA activated caspase-dependent apoptotic cell death, suggesting that mitochondria-related apoptosis genes were involved in programmed cell death. The unfolded protein response signaling pathway-related proteins were also activated on 11-epi-SA treatment, and these changes were accompanied by the upregulated expression of growth arrest and DNA damage-inducible protein (GADD153) and CCAAT/enhancer binding protein (C/EBP) homologous protein (CHOP), the genes encoding transcription factors associated with growth arrest and apoptosis under prolonged ER stress. Two inhibitors, namely salubrinal (Sal) and SP600125, partially abrogated 11-epi-SA-related cell death, implying that the protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK)–activating transcription factor (ATF) 6–CHOP or the inositol-requiring enzyme 1 alpha (IRE1α)–c-Jun N-terminal kinase (JNK)–cJun signal pathway was activated after 11-epi-SA treatment. In general, these results suggest that 11-epi-SA exerts cytotoxic effects on HA22T cells through mitochondrial dysfunction and ER stress cell death pathways. Full article

(This article belongs to the Special Issue Modulators of Endoplasmic Reticulum Stress 2016)

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18 pages, 513 KiB

Open AccessArticle

Predicting Protein–Protein Interaction Sites Using Sequence Descriptors and Site Propensity of Neighboring Amino Acids

by Tzu-Hao Kuo¹ and Kuo-Bin Li^1,2,*

¹ Institute of Biomedical Informatics, National Yang-Ming University, Taipei 112, Taiwan

² Office of Information Management, National Yang-Ming University Hospital, Yilan 260, Taiwan

Int. J. Mol. Sci. 2016, 17(11), 1788; https://doi.org/10.3390/ijms17111788 - 26 Oct 2016

Cited by 16 | Viewed by 5864

Abstract

Information about the interface sites of Protein–Protein Interactions (PPIs) is useful for many biological research works. However, despite the advancement of experimental techniques, the identification of PPI sites still remains as a challenging task. Using a statistical learning technique, we proposed a computational [...] Read more.

Information about the interface sites of Protein–Protein Interactions (PPIs) is useful for many biological research works. However, despite the advancement of experimental techniques, the identification of PPI sites still remains as a challenging task. Using a statistical learning technique, we proposed a computational tool for predicting PPI interaction sites. As an alternative to similar approaches requiring structural information, the proposed method takes all of the input from protein sequences. In addition to typical sequence features, our method takes into consideration that interaction sites are not randomly distributed over the protein sequence. We characterized this positional preference using protein complexes with known structures, proposed a numerical index to estimate the propensity and then incorporated the index into a learning system. The resulting predictor, without using structural information, yields an area under the ROC curve (AUC) of 0.675, recall of 0.597, precision of 0.311 and accuracy of 0.583 on a ten-fold cross-validation experiment. This performance is comparable to the previous approach in which structural information was used. Upon introducing the B-factor data to our predictor, we demonstrated that the AUC can be further improved to 0.750. The tool is accessible at http://bsaltools.ym.edu.tw/predppis. Full article

(This article belongs to the Special Issue Proteins and Protein-Ligand Interactions)

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18 pages, 4193 KiB

Open AccessArticle

Tomato Juice Consumption Modifies the Urinary Peptide Profile in Sprague-Dawley Rats with Induced Hepatic Steatosis

by Gala Martín-Pozuelo^1,2,*, Rocío González-Barrio^1,2

, Gonzalo G. Barberá³, Amaya Albalat^4,†, Javier García-Alonso^1,2, William Mullen⁴, Harald Mischak^4,5 and María Jesús Periago^1,2

¹ Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary, Regional Campus of International Excellence “Campus Mare Nostrum, University of Murcia, Murcia 30071, Spain

² Biomedical Research Institute of Murcia (IMIB-Arrixaca-UMU), University Clinical Hospital “Virgen de la Arrixaca”, University of Murcia, Murcia 30120, Spain

³ Department of Soil and Water Conservation, CSIC-CEBAS, Campus Universitario, Murcia 30100, Spain

⁴ Institute of Cardiovascular and Medical Science, University of Glasgow, Glasgow G12 8TA, UK

⁵ Mosaiques Diagnostics GmbH, Hannover 30659, Germany

^† Current Address: School of Natural Sciences, University of Stirling, Scotland FK9 4LA, UK

Int. J. Mol. Sci. 2016, 17(11), 1789; https://doi.org/10.3390/ijms17111789 - 26 Oct 2016

Cited by 10 | Viewed by 5838

Abstract

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in Western countries, with a high prevalence, and has been shown to increase the risk of type 2 diabetes, cardiovascular disease (CVD), etc. Tomato products contain several natural antioxidants, including lycopene—which has [...] Read more.

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in Western countries, with a high prevalence, and has been shown to increase the risk of type 2 diabetes, cardiovascular disease (CVD), etc. Tomato products contain several natural antioxidants, including lycopene—which has displayed a preventive effect on the development of steatosis and CVD. Accordingly, the aim of the present work was to evaluate the effect of tomato juice consumption on the urinary peptide profile in rats with NAFLD induced by an atherogenic diet and to identify potential peptide biomarkers for diagnosis. Urine samples, collected weekly for four weeks, were analyzed by capillary electrophoresis (CE) coupled to a mass spectrometer (MS). A partial least squares-discriminant analysis (PLS-DA) was carried out to explore the association between differential peptides and treatments. Among the 888 peptides initially identified, a total of 55 were obtained as potential biomarkers. Rats with steatosis after tomato juice intake showed a profile intermediate between that of healthy rats and that of rats with induced hepatic steatosis. Accordingly, tomato products could be considered as a dietary strategy for the impairment of NAFLD, although further research should be carried out to develop a specific biomarkers panel for NAFLD. Full article

(This article belongs to the Section Bioactives and Nutraceuticals)

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16 pages, 3876 KiB

Open AccessArticle

Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide)

by Solomon Mengistu Lemma¹, Frédéric Bossard¹ and Marguerite Rinaudo^2,*

¹ Laboratoire Rhéologie et Procédés (LRP), University of Grenoble Alpes-CNRS, 38000 Grenoble, France

² Biomaterials Applications, 6 Rue Lesdiguières, 38000 Grenoble, France

Int. J. Mol. Sci. 2016, 17(11), 1790; https://doi.org/10.3390/ijms17111790 - 26 Oct 2016

Cited by 120 | Viewed by 10040

Abstract

Electrospinning was employed to obtain chitosan nanofibers from blends of chitosans (CS) and poly(ethylene oxide) (PEO). Blends of chitosan (M_W (weight-average molecular weight) = 102 kg/mol) and PEO (M (molecular weight) = 1000 kg/mol) were selected to optimize the electrospinning process [...] Read more.

Electrospinning was employed to obtain chitosan nanofibers from blends of chitosans (CS) and poly(ethylene oxide) (PEO). Blends of chitosan (M_W (weight-average molecular weight) = 102 kg/mol) and PEO (M (molecular weight) = 1000 kg/mol) were selected to optimize the electrospinning process parameters. The PEO powder was solubilized into chitosan solution at different weight ratios in 0.5 M acetic acid. The physicochemical changes of the nanofibers were determined by scanning electron microscopy (SEM), swelling capacity, and nuclear magnetic resonance (NMR) spectroscopy. For stabilization, the produced nanofibers were neutralized with K₂CO₃ in water or 70% ethanol/30% water as solvent. Subsequently, repeated washings with pure water were performed to extract PEO, potassium acetate and carbonate salts formed in the course of chitosan nanofiber purification. The increase of PEO content in the blend from 20 to 40 w% exhibited bead-free fibers with average diameters 85 ± 19 and 147 ± 28 nm, respectively. Their NMR analysis proved that PEO and the salts were nearly completely removed from the nanostructure of chitosan, demonstrating that the adopted strategy is successful for producing pure chitosan nanofibers. In addition, the nanofibers obtained after neutralization in ethanol-aqueous solution has better structural stability, at least for six months in aqueous solutions (phosphate buffer (PBS) or water). Full article

(This article belongs to the Special Issue Chitins 2016)

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15 pages, 2405 KiB

Open AccessArticle

Preoperative 3D FSE T1-Weighted MR Plaque Imaging for Severely Stenotic Cervical ICA: Accuracy of Predicting Emboli during Carotid Endarterectomy

by Yasushi Ogasawara¹, Yuiko Sato¹, Shinsuke Narumi²

, Makoto Sasaki³, Shunrou Fujiwara¹, Masakazu Kobayashi¹, Kenji Yoshida¹, Yasuo Terayama² and Kuniaki Ogasawara^1,*

¹ Department of Neurosurgery, School of Medicine, Iwate Medical University, 19-1 Uchimaru, Morioka 020-8505, Japan

² Department of Neurology and Gerontology, School of Medicine, Iwate Medical University, 19-1 Uchimaru, Morioka 020-8505, Japan

³ Division of Ultra-High Field MRI and Department of Radiology, School of Medicine, Iwate Medical University, 19-1 Uchimaru, Morioka 020-8505, Japan

Int. J. Mol. Sci. 2016, 17(11), 1791; https://doi.org/10.3390/ijms17111791 - 27 Oct 2016

Cited by 3 | Viewed by 4449

Abstract

The aim of the present study was to determine whether preoperative three-dimensional (3D) fast spin-echo (FSE) T1-weighted magnetic resonance (MR) plaque imaging for severely stenotic cervical carotid arteries could accurately predict the development of artery-to-artery emboli during exposure of the carotid arteries in [...] Read more.

The aim of the present study was to determine whether preoperative three-dimensional (3D) fast spin-echo (FSE) T1-weighted magnetic resonance (MR) plaque imaging for severely stenotic cervical carotid arteries could accurately predict the development of artery-to-artery emboli during exposure of the carotid arteries in carotid endarterectomy (CEA). Seventy-five patients underwent preoperative MR plaque imaging and CEA under transcranial Doppler ultrasonography of the ipsilateral middle cerebral artery. On reformatted axial MR image slices showing the maximum plaque occupation rate (POR) and maximum plaque intensity for each patient, the contrast ratio (CR) was calculated by dividing the internal carotid artery plaque signal intensity by the sternocleidomastoid muscle signal intensity. For all patients, the area under the receiver operating characteristic curve (AUC)—used to discriminate between the presence and absence of microembolic signals—was significantly greater for the CR on the axial image with maximum plaque intensity (CR_{max intensity}) (0.941) than for that with the maximum POR (0.885) (p < 0.05). For 32 patients in whom both the maximum POR and the maximum plaque density were identified, the AUCs for the CR were 1.000. Preoperative 3D FSE T1-weighted MR plaque imaging accurately predicts the development of artery-to-artery emboli during exposure of the carotid arteries in CEA. Full article

(This article belongs to the Special Issue Atherosclerosis and Vascular Imaging 2016)

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17 pages, 3672 KiB

Open AccessArticle

Tocotrienol Nanoemulsion Platform of Curcumin Elicit Elevated Apoptosis and Augmentation of Anticancer Efficacy against Breast and Ovarian Carcinomas

by Nelson Steuber¹, Kathy Vo², Ritambhara Wadhwa², Jordan Birch², Paulina Iacoban¹, Pedro Chavez³ and Tamer A. Elbayoumi^1,*

¹ Department of Pharmaceutical Sciences/Nanomedicine Center of Excellence in Translational Cancer Research (Nanomedicine COE-TCR), College of Pharmacy-Glendale, Midwestern University, Glendale Hall 236-14, 19555 N. 59th Ave., Glendale, AZ 85308, USA

² Arizona College of Osteopathic Medicine, Midwestern University, 19555 N. 59th Ave., Glendale, AZ 85308, USA

³ Department of Biomedical Sciences, College of Health Sciences, Midwestern University, 19555 N. 59th Ave., Glendale, AZ 85308, USA

Int. J. Mol. Sci. 2016, 17(11), 1792; https://doi.org/10.3390/ijms17111792 - 26 Oct 2016

Cited by 23 | Viewed by 6054

Abstract

Vitamin E (VE) tocotrienols (T3), recognized for their cancer-specific anti-proliferative and pro-apoptotic activities, have been previously fabricated into bio-active nanoemulsion (NE) formulations. Here, our viscosity-adapted δ-T3 NE platform was developed to additionally incorporate curcumin (CUR), which is known for its potent suppression of [...] Read more.

Vitamin E (VE) tocotrienols (T3), recognized for their cancer-specific anti-proliferative and pro-apoptotic activities, have been previously fabricated into bio-active nanoemulsion (NE) formulations. Here, our viscosity-adapted δ-T3 NE platform was developed to additionally incorporate curcumin (CUR), which is known for its potent suppression of signaling pathways involved in malignant cell growth, survival and metastasis. Thanks to efficient 70:30 wt % surfactant mix of Lutrol F-127:VE-TPGS, in conjunction with optimal CUR loading, a prototype CUR in δ-T3 NE was successfully prepared. Model CUR/δ-T3 NE demonstrated excellent nano-scale aspects (mean particle size = 261 nm, PDI = 0.27, and ζ-potential = −35 mV), pharmaceutical stability, and controlled release properties. Suitability for systemic administration was also verified via standardized in vitro biocompatibility and hemocompatibility assays. In two human cancer cells (MCF-7 and OVCAR-8), our CUR/δ-T3 NE prominently suppressed constitutive NF-κB activation, and significantly induced apoptosis. Finally, the combined CUR/δ-T3 NE produced superior cytotoxicity profiles, in concentration- and time-dependent manners (p ≤ 0.05), at least three to four folds lower IC₅₀ than in closest CUR control. The strong synergism, estimated in both cultured carcinomas, revealed the augmented therapeutic efficacy of our CUR/δ-T3 NE combined platform, supporting its strong potential towards pharmaceutical development for cancer therapy. Full article

(This article belongs to the Special Issue Tocopherols and Tocotrienols: Metabolism and Properties)

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1 pages, 146 KiB

Open AccessCorrection

Correction: Ping Meng, et al. Involvement of the Interleukin-23/Interleukin-17 Axis in Chronic Hepatitis C Virus Infection and Its Treatment Responses. Int. J. Mol. Sci. 2016, 17, 1070

by Ping Meng¹, Suxian Zhao¹, Xuemin Niu¹, Na Fu¹, Shanshan Su¹, Rongqi Wang¹, Yuguo Zhang¹, Yuemin Nan^1,* and Liang Qiao^2,*

¹ Department of Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, Shijiazhuang 050000, China

² Storr Liver Centre, Westmead Institute for Medical Research (WIMR), the University of Sydney at Westmead Hospital, Westmead, NSW 2145, Australia

Int. J. Mol. Sci. 2016, 17(11), 1793; https://doi.org/10.3390/ijms17111793 - 26 Oct 2016

Cited by 2 | Viewed by 3184

Abstract

n/a Full article

24 pages, 3849 KiB

Open AccessArticle

Comparative Proteomic and Physiological Analysis Reveals the Variation Mechanisms of Leaf Coloration and Carbon Fixation in a Xantha Mutant of Ginkgo biloba L.

by Xinliang Liu^1,†

, Wanwen Yu^1,†, Guibin Wang¹, Fuliang Cao^1,*

, Jinfeng Cai^1,2 and Huanli Wang³

¹ Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

² The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210037, China

³ Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210037, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1794; https://doi.org/10.3390/ijms17111794 - 27 Oct 2016

Cited by 35 | Viewed by 8063

Abstract

Yellow-green leaf mutants are common in higher plants, and these non-lethal chlorophyll-deficient mutants are ideal materials for research on photosynthesis and plant development. A novel xantha mutant of Ginkgo biloba displaying yellow-colour leaves (YL) and green-colour leaves (GL) was identified in this study. [...] Read more.

Yellow-green leaf mutants are common in higher plants, and these non-lethal chlorophyll-deficient mutants are ideal materials for research on photosynthesis and plant development. A novel xantha mutant of Ginkgo biloba displaying yellow-colour leaves (YL) and green-colour leaves (GL) was identified in this study. The chlorophyll content of YL was remarkably lower than that in GL. The chloroplast ultrastructure revealed that YL had less dense thylakoid lamellae, a looser structure and fewer starch grains than GL. Analysis of the photosynthetic characteristics revealed that YL had decreased photosynthetic activity with significantly high nonphotochemical quenching. To explain these phenomena, we analysed the proteomic differences in leaves and chloroplasts between YL and GL of ginkgo using two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF/TOF MS. In total, 89 differential proteins were successfully identified, 82 of which were assigned functions in nine metabolic pathways and cellular processes. Among them, proteins involved in photosynthesis, carbon fixation in photosynthetic organisms, carbohydrate/energy metabolism, amino acid metabolism, and protein metabolism were greatly enriched, indicating a good correlation between differentially accumulated proteins and physiological changes in leaves. The identifications of these differentially accumulated proteins indicates the presence of a specific different metabolic network in YL and suggests that YL possess slower chloroplast development, weaker photosynthesis, and a less abundant energy supply than GL. These studies provide insights into the mechanism of molecular regulation of leaf colour variation in YL mutants. Full article

(This article belongs to the Special Issue Plant Proteomic Research)

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24 pages, 2129 KiB

Open AccessReview

Function and Regulation of MicroRNAs and Their Potential as Biomarkers in Paediatric Liver Disease

by Diego A. Calvopina¹, Miranda A. Coleman¹, Peter J. Lewindon^1,2,3 and Grant A. Ramm^1,3,*

¹ Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 300 Herston Rd, Herston, QLD 4006, Australia

² Department of Gastroenterology and Hepatology, Lady Cilento Children′s Hospital, 501 Stanley St, South Brisbane, QLD 4101, Australia

³ Faculty of Medicine and Biomedical Sciences, The University of Queensland, Brisbane, QLD 4006, Australia

Int. J. Mol. Sci. 2016, 17(11), 1795; https://doi.org/10.3390/ijms17111795 - 27 Oct 2016

Cited by 29 | Viewed by 7794

Abstract

MicroRNAs (miRNAs) are short non-coding RNAs involved in biological and pathological processes of every cell type, including liver cells. Transcribed from specific genes, miRNA precursors are processed in the cytoplasm into mature miRNAs and as part of the RNA-induced silencing complex (RISC) complex [...] Read more.

MicroRNAs (miRNAs) are short non-coding RNAs involved in biological and pathological processes of every cell type, including liver cells. Transcribed from specific genes, miRNA precursors are processed in the cytoplasm into mature miRNAs and as part of the RNA-induced silencing complex (RISC) complex binds to messenger RNA (mRNA) by imperfect complementarity. This leads to the regulation of gene expression at a post-transcriptional level. The function of a number of different miRNAs in fibrogenesis associated with the progression of chronic liver disease has recently been elucidated. Furthermore, miRNAs have been shown to be both disease-and tissue-specific and are stable in the circulation, which has led to increasing investigation on their utility as biomarkers for the diagnosis of chronic liver diseases, including those in children. Here, we review the current knowledge on the biogenesis of microRNA, the mechanisms of translational repression and the use of miRNA as circulatory biomarkers in chronic paediatric liver diseases including cystic fibrosis associated liver disease, biliary atresia and viral hepatitis B. Full article

(This article belongs to the Special Issue microRNA Regulation 2017)

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13 pages, 5037 KiB

Open AccessArticle

Molecular Dynamic Studies of the Complex Polyethylenimine and Glucose Oxidase

by Beata Szefler^1,*

, Mircea V. Diudea², Mihai V. Putz^3,4,* and Ireneusz P. Grudzinski⁵

¹ Department of Physical Chemistry, Faculty of Pharmacy, Collegium Medicum, Nicolaus Copernicus University, Kurpinskiego 5, 85-096 Bydgoszcz, Poland

² Department of Chemistry, Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 400028 Cluj-Napoca, Romania

³ Laboratory of Structural and Computational Physical-Chemistry for Nanosciences and QSAR, Biology-Chemistry Department, West University of Timisoara, Str. Pestalozzi No. 16, 300115 Timisoara, Romania

⁴ Laboratory of Renewable Energies-Photovoltaics, R&D National Institute for Electrochemistry and Condensed Matter (INCEMC), Dr. A. Paunescu Podeanu Str. No. 144, 300569 Timisoara, Romania

⁵ Department of Pharmaceutical Nanotechnology and Nanotoxicology, Faculty of Pharmacy, Medical University of Warsaw, 02-097 Warsaw, Poland

Int. J. Mol. Sci. 2016, 17(11), 1796; https://doi.org/10.3390/ijms17111796 - 27 Oct 2016

Cited by 12 | Viewed by 7000

Abstract

Glucose oxidase (GOx) is an enzyme produced by Aspergillus, Penicillium and other fungi species. It catalyzes the oxidation of β-d-glucose (by the molecular oxygen or other molecules, like quinones, in a higher oxidation state) to form d-glucono-1,5-lactone, which hydrolyses spontaneously [...] Read more.

Glucose oxidase (GOx) is an enzyme produced by Aspergillus, Penicillium and other fungi species. It catalyzes the oxidation of β-d-glucose (by the molecular oxygen or other molecules, like quinones, in a higher oxidation state) to form d-glucono-1,5-lactone, which hydrolyses spontaneously to produce gluconic acid. A coproduct of this enzymatic reaction is hydrogen peroxide (H₂O₂). GOx has found several commercial applications in chemical and pharmaceutical industries including novel biosensors that use the immobilized enzyme on different nanomaterials and/or polymers such as polyethylenimine (PEI). The problem of GOx immobilization on PEI is retaining the enzyme native activity despite its immobilization onto the polymer surface. Therefore, the molecular dynamic (MD) study of the PEI ligand (C14N8_07_B22) and the GOx enzyme (3QVR) was performed to examine the final complex PEI-GOx stabilization and the affinity of the PEI ligand to the docking sites of the GOx enzyme. The docking procedure showed two places/regions of major interaction of the protein with the polymer PEI: (LIG1) of −5.8 kcal/mol and (LIG2) of −4.5 kcal/mol located inside the enzyme and on its surface, respectively. The values of enthalpy for the PEI-enzyme complex, located inside of the protein (LIG1) and on its surface (LIG2) were computed. Docking also discovered domains of the GOx protein that exhibit no interactions with the ligand or have even repulsive characteristics. The structural data clearly indicate some differences in the ligand PEI behavior bound at the two places/regions of glucose oxidase. Full article

(This article belongs to the Special Issue Computational Eco, Bio, and Pharmaco-Toxicity 2016)

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9 pages, 1252 KiB

Open AccessArticle

The Activating NKG2C Receptor Is Significantly Reduced in NK Cells after Allogeneic Stem Cell Transplantation in Patients with Severe Graft-versus-Host Disease

by Lambros Kordelas^1,*

, Nina-Kristin Steckel¹, Peter A. Horn², Dietrich W. Beelen¹ and Vera Rebmann²

¹ Department of Bone Marrow Transplantation, University Hospital Essen, Essen 45147, Germany

² Institute for Transfusion Medicine, University Hospital Essen, Essen 45147, Germany

Int. J. Mol. Sci. 2016, 17(11), 1797; https://doi.org/10.3390/ijms17111797 - 27 Oct 2016

Cited by 33 | Viewed by 5584

Abstract

Natural killer (NK) cells play a central role in the innate immune system. In allogeneic stem cell transplantation (alloSCT), alloreactive NK cells derived by the graft are discussed to mediate the elimination of leukemic cells and dendritic cells in the patient and thereby [...] Read more.

Natural killer (NK) cells play a central role in the innate immune system. In allogeneic stem cell transplantation (alloSCT), alloreactive NK cells derived by the graft are discussed to mediate the elimination of leukemic cells and dendritic cells in the patient and thereby to reduce the risk for leukemic relapses and graft-versus-host reactions. The alloreactivity of NK cells is determined by various receptors including the activating CD94/NKG2C and the inhibitory CD94/NKG2A receptors, which both recognize the non-classical human leukocyte antigen E (HLA-E). Here we analyze the contribution of these receptors to NK cell alloreactivity in 26 patients over the course of the first year after alloSCT due to acute myeloid leukemia, myelodysplastic syndrome and T cell Non-Hodgkin-Lymphoma. Our results show that NK cells expressing the activating CD94/NKG2C receptor are significantly reduced in patients after alloSCT with severe acute and chronic graft-versus-host disease (GvHD). Moreover, the ratio of CD94/NKG2C to CD94/NKG2A was reduced in patients with severe acute and chronic GvHD after receiving an HLA-mismatched graft. Collectively, these results provide evidence for the first time that CD94/NKG2C is involved in GvHD prevention. Full article

(This article belongs to the Special Issue Advances in Cell Transplantation)

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12 pages, 5537 KiB

Open AccessArticle

Effects of Ganodermanondiol, a New Melanogenesis Inhibitor from the Medicinal Mushroom Ganoderma lucidum

by Ji-Woong Kim^†, Hong-Il Kim^†, Jong-Hyeon Kim, O-Chul Kwon, Eun-Suk Son, Chang-Soo Lee and Young-Jin Park^*

¹ Department of Biomedical Chemistry, Konkuk University, Chungju 27478, Korea

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1798; https://doi.org/10.3390/ijms17111798 - 27 Oct 2016

Cited by 38 | Viewed by 9821

Abstract

Ganoderma lucidum, a species of the Basidiomycetes class, has been attracting international attention owing to its wide variety of biological activities and great potential as an ingredient in skin care cosmetics including “skin-whitening” products. However, there is little information available on its [...] Read more.

Ganoderma lucidum, a species of the Basidiomycetes class, has been attracting international attention owing to its wide variety of biological activities and great potential as an ingredient in skin care cosmetics including “skin-whitening” products. However, there is little information available on its inhibitory effect against tyrosinase activity. Therefore, the objectives of this study were to investigate the chemical composition of G. lucidum and its inhibitory effects on melanogenesis. We isolated the active compound from G. lucidum using ethanol extraction and ethyl acetate fractionation. In addition, we assayed its inhibitory effects on tyrosinase activity and melanin biosynthesis in B16F10 melanoma cells. In this study, we identified a bioactive compound, ganodermanondiol, which inhibits the activity and expression of cellular tyrosinase and the expression of tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF), thereby decreasing melanin production. Furthermore, ganodermanondiol also affected the mitogen-activated protein kinase (MAPK) cascade and cyclic adenosine monophosphate (cAMP)-dependent signaling pathway, which are involved in the melanogenesis of B16F10 melanoma cells. The finding that ganodermanondiol from G. lucidum exerts an inhibitory effect on tyrosinase will contribute to the use of this mushroom in the preparation of skin care products in the future. Full article

(This article belongs to the Special Issue Biochemistry and Mechanisms of Melanogenesis)

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14 pages, 5114 KiB

Open AccessArticle

miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways

by Hang Song¹, Yao Zhang¹, Na Liu¹, Sheng Zhao², Yan Kong² and Liudi Yuan^1,2,*

¹ State Education Ministry’s Key Laboratory of Developmental Genes and Human Diseases, Southeast University, No. 2 Sipailou Road, Nanjing 210096, China

² Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing 210009, China

Int. J. Mol. Sci. 2016, 17(11), 1799; https://doi.org/10.3390/ijms17111799 - 27 Oct 2016

Cited by 45 | Viewed by 6528

Abstract

MicroRNAs (miRNAs) are implicated in the regulation of tumor progression and stemness of cancer stem-like cells. Recently, miR-92a-3p was reported to be up-regulated in human glioma samples. Nevertheless, the precise role of miR-92a-3p in glioma cells and glioma stem-like cells (GSCs) has not [...] Read more.

MicroRNAs (miRNAs) are implicated in the regulation of tumor progression and stemness of cancer stem-like cells. Recently, miR-92a-3p was reported to be up-regulated in human glioma samples. Nevertheless, the precise role of miR-92a-3p in glioma cells and glioma stem-like cells (GSCs) has not been fully elucidated. It is necessary to clarify the function of miR-92a-3p in glioma and GSCs to develop novel therapeutic approaches for glioma patients. In the present study, we applied methyl-thiazolyl-tetrazolium (MTT) assay and Transwell assay to measure the proliferation rate and metastatic potential of glioma cells. Meanwhile, the self-renewal ability of GSCs was detected by tumor sphere formation assay. The results revealed that down-regulation of miR-92a-3p suppressed the glioma cell malignancy in vitro. Moreover, knockdown of miR-92a-3p led to a reduction of tumorgenesis in vivo. Interestingly, we also observed that up-regulation of miR-92a-3p could inhibit the stemness of GSCs. Subsequent mechanistic investigation indicated that cadherin 1 (CDH1)/β-catenin signaling and Notch-1/Akt signaling were the downstream pathways of miR-92a-3p in glioma cells and GSCs, respectively. These results suggest that miR-92a-3p plays different roles in glioma cells and GSCs through regulating different signaling pathways. Full article

(This article belongs to the Special Issue microRNA Regulation 2017)

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Open AccessArticle

Urinary Angiotensinogen Could Be a Prognostic Marker of the Renoprotection of Olmesartan in Metabolic Syndrome Patients

by Tomoko Mizushige¹, Hiroyuki Kobori^1,2,3,*

, Hirofumi Hitomi¹, Yoko Nishijima⁴, Fumihiro Tomoda⁵, Satoshi Morimoto⁶, Masakazu Kohno⁴ and Akira Nishiyama¹

¹ Department of Pharmacology, Kagawa University School of Medicine, Kagawa 761-0793, Japan

² Departments of Pharmacology and of Nephrology, School of Medicine, International University of Health and Welfare, Tokyo 107-0062, Japan

³ Departments of Physiology and of Medicine, Tulane University Health Sciences Center, New Orleans, LA 70112, USA

⁴ Department of Medicine, Kagawa University School of Medicine, Kagawa 761-0793, Japan

⁵ The Second Department of Internal Medicine, Toyama University School of Medicine, Toyama 930-0194, Japan

⁶ The Second Department of Internal Medicine, Kansai Medical University, Osaka 573-1010, Japan

Int. J. Mol. Sci. 2016, 17(11), 1800; https://doi.org/10.3390/ijms17111800 - 27 Oct 2016

Cited by 3 | Viewed by 4835

Abstract

This study was performed to demonstrate urinary angiotensinogen as a potential prognostic marker of the albuminuria reduction effects of olmesartan in patients with metabolic syndrome. In 24 patients (eight women, 57.88 ± 2.00 years), 5–40 mg/day of olmesartan were given. Urinary concentrations of [...] Read more.

This study was performed to demonstrate urinary angiotensinogen as a potential prognostic marker of the albuminuria reduction effects of olmesartan in patients with metabolic syndrome. In 24 patients (eight women, 57.88 ± 2.00 years), 5–40 mg/day of olmesartan were given. Urinary concentrations of albumin and angiotensinogen (normalized by urinary concentrations of creatinine) and plasma renin activity were measured before and after the 12- and 24-week marks of olmesartan treatment. Olmesartan treatment increased plasma renin activity and decreased urinary albumin and urinary angiotensinogen significantly (p < 0.05). Based on the % change in urinary albumin, patients were divided into two groups, responders (<−50%) and non-responders (≥−50%), and a logistic analysis of urinary angiotensinogen before treatment showed the area under the curve as 0.694. When the cutoff value of urinary angiotensinogen before the treatment of 13.9 µg/g Cr was used, the maximum Youden index (0.500, specificity: 11/12 = 91.7% and sensitivity: 7/12 = 58.3%) was obtained. When all patients were re-divided into two groups, those with higher values of urinary angiotensinogen before the treatment (Group H, n = 16) and those with lower values, Group H showed significantly decreased urinary albumin (p < 0.05). Therefore, urinary angiotensinogen could be a prognostic marker of the albuminuria reduction effects of olmesartan in patients with metabolic syndrome. Full article

(This article belongs to the Special Issue Advances in Chronic Kidney Disease 2017)

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16 pages, 1108 KiB

Open AccessReview

Extracellular Vesicles in Chronic Obstructive Pulmonary Disease

by Tsukasa Kadota^1,2, Yu Fujita^1,2, Yusuke Yoshioka¹, Jun Araya², Kazuyoshi Kuwano² and Takahiro Ochiya^1,*

¹ Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo 104-0045, Japan

² Division of Respiratory Disease, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan

Int. J. Mol. Sci. 2016, 17(11), 1801; https://doi.org/10.3390/ijms17111801 - 27 Oct 2016

Cited by 65 | Viewed by 10359

Abstract

Chronic obstructive pulmonary disease (COPD) is characterized by the progression of irreversible airflow limitation and is a leading cause of morbidity and mortality worldwide. Although several crucial mechanisms of COPD pathogenesis have been studied, the precise mechanism remains unknown. Extracellular vesicles (EVs), including [...] Read more.

Chronic obstructive pulmonary disease (COPD) is characterized by the progression of irreversible airflow limitation and is a leading cause of morbidity and mortality worldwide. Although several crucial mechanisms of COPD pathogenesis have been studied, the precise mechanism remains unknown. Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, are released from almost all cell types and are recognized as novel cell–cell communication tools. They have been shown to carry and transfer a wide variety of molecules, such as microRNAs, messenger RNAs, and proteins, which are involved in physiological functions and the pathology of various diseases. Recently, EVs have attracted considerable attention in pulmonary research. In this review, we summarize the recent findings of EV-mediated COPD pathogenesis. We also discuss the potential clinical usefulness of EVs as biomarkers and therapeutic agents for the treatment of COPD. Full article

(This article belongs to the Special Issue Focus on Extracellular Vesicles)

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8 pages, 493 KiB

Open AccessArticle

A Clinical Trial about a Food Supplement Containing α-Lipoic Acid on Oxidative Stress Markers in Type 2 Diabetic Patients

by Giuseppe Derosa^1,2,3,4,*

, Angela D’Angelo^1,2,4, Davide Romano¹ and Pamela Maffioli^1,3,5

¹ Centre of Diabetes and Metabolic Diseases, Department of Internal Medicine and Therapeutics, University of Pavia and Fondazione IRCCS Policlinico S. Matteo, 2-27100 Pavia, Italy

² Center for Prevention, Surveillance, Diagnosis and Treatment of Rare Diseases, Fondazione IRCCS Policlinico S. Matteo, 2-27100 Pavia, Italy

³ Center for the Study of Endocrine-Metabolic Pathophysiology and Clinical Research, University of Pavia, 2-27100 Pavia, Italy

⁴ Laboratory of Molecular Medicine, University of Pavia, 2-27100 Pavia, Italy

⁵ PhD School in Experimental Medicine, University of Pavia, 2-27100 Pavia, Italy

Int. J. Mol. Sci. 2016, 17(11), 1802; https://doi.org/10.3390/ijms17111802 - 28 Oct 2016

Cited by 64 | Viewed by 9912

Abstract

The aim of this study was to evaluate the effect of a food supplement containing α-lipoic acid and of a placebo on glyco-metabolic control and on oxidative stress markers in type 2 diabetics. We randomized 105 diabetics to either a supplementation containing 600 [...] Read more.

The aim of this study was to evaluate the effect of a food supplement containing α-lipoic acid and of a placebo on glyco-metabolic control and on oxidative stress markers in type 2 diabetics. We randomized 105 diabetics to either a supplementation containing 600 mg of α-lipoic acid, 165 mg of L-carnosin, 7.5 mg of zinc, and vitamins of group B, or a placebo, for three months. We evaluated body mass index, fasting plasma glucose (FPG), post-prandial-glucose (PPG), glycated hemoglobin (HbA_1c), fasting plasma insulin (FPI), HOMA-index (HOMA-IR), lipid profile, high sensitivity C-reactive protein (Hs-CRP), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA). There was a reduction of FPG, PPG, and HbA_1c with the food supplement containing α-lipoic acid compared with a baseline, and with the placebo. Concerning lipid profile, we observed a reduction of LDL-C, and Tg with the food supplement, compared with both the baseline, and the placebo. There was a reduction of Hs-CRP with the food supplement containing α-lipoic acid, both compared with the baseline and the placebo. An increase of SOD, and GSH-Px, and a decrease of MDA were reached by the food supplement containing α-lipoic acid, both compared with the baseline and the placebo. We can conclude that the food supplement containing α-lipoic acid, L-carnosin, zinc, and vitamins of group B improved glycemic control, lipid profile, and anti-oxidative stress markers. Full article

(This article belongs to the Special Issue Advances in Molecular Research of Functional and Nutraceutical Food 2017)

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10 pages, 1586 KiB

Open AccessArticle

The Expression and Clinical Outcome of pCHK2-Thr68 and pCDC25C-Ser216 in Breast Cancer

by Huayong Jiang^1,†, Bin Wang^2,†, Fuli Zhang¹, Yuanyu Qian³, Chia-Chen Chuang⁴, Mingzhen Ying², Yajie Wang^2,* and Li Zuo^4,*

¹ Department of Radiation Oncology, PLA Army General Hospital, Beijing 100700, China

² Department of Oncology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China

³ Department of Emergency, Chinese PLA General Hospital, Beijing 100853, China

⁴ Radiologic Sciences and Respiratory Therapy Division, School of Health and Rehabilitation Sciences, The Ohio State University College of Medicine, Columbus, OH 43210, USA

^† These authors contributed equally to the work.

Int. J. Mol. Sci. 2016, 17(11), 1803; https://doi.org/10.3390/ijms17111803 - 28 Oct 2016

Cited by 5 | Viewed by 5530

Abstract

Checkpoint kinase 2 (CHK2) and cell division cycle 25C (CDC25C) are two proteins involved in the DNA damage response pathway, playing essential roles in maintaining genome integrity. As one of the major hallmarks of abnormal cellular division, genomic instability occurs in most cancers. [...] Read more.

Checkpoint kinase 2 (CHK2) and cell division cycle 25C (CDC25C) are two proteins involved in the DNA damage response pathway, playing essential roles in maintaining genome integrity. As one of the major hallmarks of abnormal cellular division, genomic instability occurs in most cancers. In this study, we identified the functional expression of pCHK2-Thr68 and pCDC25C-Ser216 in breast cancer, as well as its association with breast cancer survival. Tissue microarray analysis using immunohistochemistry was constructed to identify the expression of pCHK2-Thr68 and pCDC25C-Ser216 in 292 female breast cancer patients. The relationship among protein expression, clinicopathological factors (e.g., human epidermal growth factor receptor 2 (HER 2), tumor size, tumor-node-metastasis (TNM) classification), and overall survival of the breast cancer tissues were analyzed using Pearson’s χ-square (χ²) test, Fisher’s exact test, multivariate logistic regression and Kaplan–Meier survival analysis. Significantly higher expressions of pCHK2-Thr68 and pCDC25C-Ser216 were observed in the nucleus of the breast cancer cells compared to the paracancerous tissue (pCHK2-Thr68, 20.38% vs. 0%; pCDC25C-Ser216, 82.26% vs. 24.24%). The expression of pCHK2-Thr68 and pCDC25C-Ser216 in breast cancer showed a positive linear correlation (p = 0.026). High expression of pCHK2-Thr68 was associated with decreased patient survival (p = 0.001), but was not an independent prognostic factor. Our results suggest that pCHK2-Thr68 and pCDC25C-Ser216 play important roles in breast cancer and may be potential treatment targets. Full article

(This article belongs to the Special Issue Advances in Molecular Oncology)

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12 pages, 2749 KiB

Open AccessArticle

AQP2 Plasma Membrane Diffusion Is Altered by the Degree of AQP2-S256 Phosphorylation

by Eva C. Arnspang^1,2,†

, Frédéric H. Login¹, Jennifer S. Koffman³, Prabuddha Sengupta^2,‡ and Lene N. Nejsum^1,*

¹ Department of Clinical Medicine, Aarhus University, DK-8000 Aarhus C, Denmark

² The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA

³ Department of Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus C, Denmark

^† Current address: Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.

^‡ Current address: Janelia Research Campus, Ashburn, VA 20147, USA.

Int. J. Mol. Sci. 2016, 17(11), 1804; https://doi.org/10.3390/ijms17111804 - 28 Oct 2016

Cited by 22 | Viewed by 5763

Abstract

Fine tuning of urine concentration occurs in the renal collecting duct in response to circulating levels of arginine vasopressin (AVP). AVP stimulates intracellular cAMP production, which mediates exocytosis of sub-apical vesicles containing the water channel aquaporin-2 (AQP2). Protein Kinase A (PKA) phosphorylates AQP2 [...] Read more.

Fine tuning of urine concentration occurs in the renal collecting duct in response to circulating levels of arginine vasopressin (AVP). AVP stimulates intracellular cAMP production, which mediates exocytosis of sub-apical vesicles containing the water channel aquaporin-2 (AQP2). Protein Kinase A (PKA) phosphorylates AQP2 on serine-256 (S256), which triggers plasma membrane accumulation of AQP2. This mediates insertion of AQP2 into the apical plasma membrane, increasing water permeability of the collecting duct. AQP2 is a homo-tetramer. When S256 on all four monomers is changed to the phosphomimic aspartic acid (S256D), AQP2-S256D localizes to the plasma membrane and internalization is decreased. In contrast, when S256 is mutated to alanine (S256A) to mimic non-phosphorylated AQP2, AQP2-S256A localizes to intracellular vesicles as well as the plasma membrane, with increased internalization from the plasma membrane. S256 phosphorylation is not necessary for exocytosis and dephosphorylation is not necessary for endocytosis, however, the degree of S256 phosphorylation is hypothesized to regulate the kinetics of AQP2 endocytosis and thus, retention time in the plasma membrane. Using k-space Image Correlation Spectroscopy (kICS), we determined how the number of phosphorylated to non-phosphorylated S256 monomers in the AQP2 tetramer affects diffusion speed of AQP2 in the plasma membrane. When all four monomers mimicked constitutive phosphorylation (AQP2-S256D), diffusion was faster than when all four were non-phosphorylated (AQP2-S256A). AQP2-WT diffused at a speed similar to that of AQP2-S256D. When an average of two or three monomers in the tetramer were constitutively phosphorylated, the average diffusion coefficients were not significantly different to that of AQP2-S256D. However, when only one monomer was phosphorylated, diffusion was slower and similar to AQP2-S256A. Thus, AQP2 with two to four phosphorylated monomers has faster plasma membrane kinetics, than the tetramer which contains just one or no phosphorylated monomers. This difference in diffusion rate may reflect behavior of AQP2 tetramers destined for either plasma membrane retention or endocytosis. Full article

(This article belongs to the Special Issue Aquaporin)

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14 pages, 4216 KiB

Open AccessArticle

Peculiarities of the Super-Folder GFP Folding in a Crowded Milieu

by Olesya V. Stepanenko¹, Olga V. Stepanenko¹

, Irina M. Kuznetsova¹, Vladimir N. Uversky^1,3,*

and Konstantin K. Turoverov^1,2,*

¹ Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., St. Petersburg 194064, Russia

² Institute of Physics, Nanotechnology and Telecommunications, Peter the Great St. Petersburg State Polytechnic University, 29 Polytechnicheskaya st., St. Petersburg 195251, Russia

³ Department of Molecular Medicine and USF Health Byrd Alzheimer’s Research Institute, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd. MDC07, Tampa, FL 33612, USA

Int. J. Mol. Sci. 2016, 17(11), 1805; https://doi.org/10.3390/ijms17111805 - 28 Oct 2016

Cited by 9 | Viewed by 6680

Abstract

The natural cellular milieu is crowded by large quantities of various biological macromolecules. This complex environment is characterized by a limited amount of unoccupied space, limited amounts of free water, and changed solvent properties. Obviously, such a tightly packed cellular environment is poorly [...] Read more.

The natural cellular milieu is crowded by large quantities of various biological macromolecules. This complex environment is characterized by a limited amount of unoccupied space, limited amounts of free water, and changed solvent properties. Obviously, such a tightly packed cellular environment is poorly mimicked by traditional physiological conditions, where low concentrations of a protein of interest are analyzed in slightly salted aqueous solutions. An alternative is given by the use of a model crowded milieu, where a protein of interest is immersed in a solution containing high concentrations of various polymers that serve as model crowding agents. An expected outcome of the presence of such macromolecular crowding agents is their ability to increase conformational stability of a globular protein due to the excluded volume effects. In line with this hypothesis, the behavior of a query protein should be affected by the hydrodynamic size and concentration of an inert crowder (i.e., an agent that does not interact with the protein), whereas the chemical nature of a macromolecular crowder should not play a role in its ability to modulate conformational properties. In this study, the effects of different crowding agents (polyethylene glycols (PEGs) of various molecular masses (PEG-600, PEG-8000, and PEG-12000), Dextran-70, and Ficoll-70) on the spectral properties and unfolding–refolding processes of the super-folder green fluorescent protein (sfGFP) were investigated. sfGFP is differently affected by different crowders, suggesting that, in addition to the expected excluded volume effects, there are some changes in the solvent properties. Full article

(This article belongs to the Special Issue Protein Folding)

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14 pages, 3034 KiB

Open AccessArticle

Different Susceptibilities between Apoe- and Ldlr-Deficient Mice to Inflammation-Associated Colorectal Carcinogenesis

by Takuji Tanaka^1,2,*

, Takeru Oyama³, Shigeyuki Sugie⁴

and Masahito Shimizu⁵

¹ Department of Diagnostic Pathology (DDP) and Research Center of Diagnostic Pathology (RC-DiP), Gifu Municipal Hospital, 7-1 Kashima-cho, Gifu City, Gifu 500-8513, Japan

² Department of Tumor Pathology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu City, Gifu 501-1194, Japan

³ Department of Molecular and Cellular Pathology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan

⁴ Department of Pathology, Murakami Memorial Hospital, Asahi University, School of Dentistry, 3-23 Hashimoto-cho, Gifu City, Gifu 500-8523, Japan

⁵ Department of Gastroenterology/Internal Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu City, Gifu 501-1194, Japan

Int. J. Mol. Sci. 2016, 17(11), 1806; https://doi.org/10.3390/ijms17111806 - 28 Oct 2016

Cited by 11 | Viewed by 5942

Abstract

Hypercholesterolemia resulting in atherosclerosis is associated with an increased risk of ischemic heart disease and colorectal cancer (CRC). However, the roles of apoliprotein (Apo) E (Apoe) and low-density lipoprotein (Ldl) receptor (Ldlr) in colorectal carcinogenesis have not [...] Read more.

Hypercholesterolemia resulting in atherosclerosis is associated with an increased risk of ischemic heart disease and colorectal cancer (CRC). However, the roles of apoliprotein (Apo) E (Apoe) and low-density lipoprotein (Ldl) receptor (Ldlr) in colorectal carcinogenesis have not yet been investigated. In this study, we examined the susceptibility of Apoe-deficient and Ldlr-deficient mice, which are genetic animal models of atherosclerosis to azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colorectal carcinogenesis. In Experiment 1, male Apoe-deficient (n = 20) and wild type (WT) mice (C57BL/6J, n = 21) were treated with a single intraperitoneal (i.p.) injection of AOM (10 mg/kg body weight) and then given 1.5% DSS in drinking water for seven days. They were maintained up to week 20 and sacrificed for the histopathological examination of colorectal tumors. The mRNA expression of cyclooxygenase (Cox)-2, inducible nitric oxide synthase (Nos2), tumor necrosis factor (Tnf)-α interleukin (Il)-1β, and Il-6 was assayed in the colorectal mucosa. In Experiment 2, male Ldlr-deficient (n = 14) and WT mice (C57BL/6J, n = 10) were given a single i.p. injection of AOM (10 mg/kg body weight) and then given 2% DSS in drinking water for seven days. They were sacrificed at week 20 to evaluate their colorectum histopathologically. In Experiment 1, the multiplicity of CRCs was significantly higher in the Apoe-deficient mice (2.75 ± 1.48) than in the WT mice (0.62 ± 0.67). The serum lipoprotein levels in the Apoe-deficient mice were also significantly higher than in the WT mice. In Experiment 2, the incidence (29%) and multiplicity (0.50 ± 0.94) of CRCs in the Ldlr mice were significantly lower than in the WT mice (80% incidence and 3.10 ± 2.38 multiplicity). The mRNA expression of two inducible enzymes and certain pro-inflammatory cytokines in the colorectum of each genotype was greater than in the respective WT mice. The values in the Apoe-deficient mice were much greater than in the Ldlr mice. These findings suggest that Apoe-deficient mice showed increased susceptibility to inflammation-associated colorectal carcinogenesis due to their high reactivity to inflammatory stimuli. Full article

(This article belongs to the Special Issue Inflammation and Cancer)

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15 pages, 814 KiB

Open AccessReview

The Gas6/TAM System and Multiple Sclerosis

by Mattia Bellan^1,2,3, Mario Pirisi^1,2,3 and Pier Paolo Sainaghi^2,3,*

¹ Department of Translational Medicine, Università del Piemonte Orientale, UPO, via Solaroli 17, 28100 Novara, Italy

² Immuno-rheumatology Unit, Internal Medicine Division, “Maggiore della Carità” Hospital, 28100 Novara, Italy

³ IRCAD, Interdisciplinary Research Center of Autoimmune Diseases, 28100 Novara, Italy

Int. J. Mol. Sci. 2016, 17(11), 1807; https://doi.org/10.3390/ijms17111807 - 28 Oct 2016

Cited by 41 | Viewed by 9069

Abstract

Growth arrest specific 6 (Gas6) is a multimodular circulating protein, the biological actions of which are mediated by the interaction with three transmembrane tyrosine kinase receptors: Tyro3, Axl, and MerTK, collectively named TAM. Over the last few decades, many progresses have been done [...] Read more.

Growth arrest specific 6 (Gas6) is a multimodular circulating protein, the biological actions of which are mediated by the interaction with three transmembrane tyrosine kinase receptors: Tyro3, Axl, and MerTK, collectively named TAM. Over the last few decades, many progresses have been done in the understanding of the biological activities of this highly pleiotropic system, which plays a role in the regulation of immune response, inflammation, coagulation, cell growth, and clearance of apoptotic bodies. Recent findings have further related Gas6 and TAM receptors to neuroinflammation in general and, specifically, to multiple sclerosis (MS). In this paper, we review the biology of the Gas6/TAM system and the current evidence supporting its potential role in the pathogenesis of MS. Full article

(This article belongs to the Special Issue Advances in Multiple Sclerosis 2016)

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13 pages, 1856 KiB

Open AccessArticle

PPIC, EMP3 and CHI3L1 Are Novel Prognostic Markers for High Grade Glioma

by Yuan-Feng Gao^1,2, Tao Zhu^1,2, Chen-Xue Mao^1,2, Zhi-Xiong Liu³, Zhi-Bin Wang^1,2, Xiao-Yuan Mao^1,2, Ling Li^1,2, Ji-Ye Yin^1,2, Hong-Hao Zhou^1,2 and Zhao-Qian Liu^1,2,*

¹ Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China

² Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, China

³ Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China

Int. J. Mol. Sci. 2016, 17(11), 1808; https://doi.org/10.3390/ijms17111808 - 28 Oct 2016

Cited by 41 | Viewed by 7030

Abstract

Current treatment methods for patients diagnosed with gliomas have shown limited success. This is partly due to the lack of prognostic genes available to accurately predict disease outcomes. The aim of this study was to investigate novel prognostic genes based on the molecular [...] Read more.

Current treatment methods for patients diagnosed with gliomas have shown limited success. This is partly due to the lack of prognostic genes available to accurately predict disease outcomes. The aim of this study was to investigate novel prognostic genes based on the molecular profile of tumor samples and their correlation with clinical parameters. In the current study, microarray data (GSE4412 and GSE7696) downloaded from Gene Expression Omnibus were used to identify differentially expressed prognostic genes (DEPGs) by significant analysis of microarray (SAM) between long-term survivors (>2 years) and short-term survivors (≤2 years). DEPGs generated from these two datasets were intersected to obtain a list of common DEPGs. The expression of a subset of common DEPGs was then independently validated by real-time reverse transcription quantitative PCR (qPCR). Survival value of the common DEPGs was validated using known survival data from the GSE4412 and TCGA dataset. After intersecting DEPGs generated from the above two datasets, three genes were identified which may potentially be used to determine glioma patient prognosis. Independent validation with glioma patients tissue (n = 70) and normal brain tissue (n = 19) found PPIC, EMP3 and CHI3L1 were up-regulated in glioma tissue. Survival value validation showed that the three genes correlated with patient survival by Kaplan-Meir analysis, including grades, age and therapy. Full article

(This article belongs to the Special Issue Advances in Molecular Oncology)

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18 pages, 1637 KiB

Open AccessArticle

Tocotrienol Affects Oxidative Stress, Cholesterol Homeostasis and the Amyloidogenic Pathway in Neuroblastoma Cells: Consequences for Alzheimer’s Disease

by Marcus O. W. Grimm^1,2,3,*,†

, Liesa Regner^1,†, Janine Mett¹, Christoph P. Stahlmann¹, Pascal Schorr^1,4, Christopher Nelke¹, Olga Streidenberger¹, Hannah Stoetzel¹, Jakob Winkler¹, Shatha R. Zaidan¹, Andrea Thiel¹

, Kristina Endres⁵

, Heike S. Grimm¹

, Dietrich A. Volmer⁴ and Tobias Hartmann^1,2,5

¹ Experimental Neurology, Saarland University, Kirrberger Straße 1, 66421 Homburg/Saar, Germany

² Neurodegeneration and Neurobiology, Saarland University, Kirrberger Straße 1, 66421 Homburg/Saar, Germany

³ Deutsches Institut für DemenzPrävention (DIDP), Saarland University, Kirrberger Straße 1, 66421 Homburg/Saar, Germany

⁴ Institute of Bioanalytical Chemistry, Saarland University, 66123 Saarbrücken, Germany

⁵ Department of Psychiatry and Psychotherapy, Clinical Research Group, University Medical Centre Johannes Gutenberg-University Mainz, Untere Zahlbacher Straße 8, 55131 Mainz, Germany

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1809; https://doi.org/10.3390/ijms17111809 - 29 Oct 2016

Cited by 44 | Viewed by 8169

Abstract

One of the characteristics of Alzheimer´s disease (AD) is an increased amyloid load and an enhanced level of reactive oxidative species (ROS). Vitamin E has known beneficial neuroprotective effects, and previously, some studies suggested that vitamin E is associated with a reduced risk [...] Read more.

One of the characteristics of Alzheimer´s disease (AD) is an increased amyloid load and an enhanced level of reactive oxidative species (ROS). Vitamin E has known beneficial neuroprotective effects, and previously, some studies suggested that vitamin E is associated with a reduced risk of AD due to its antioxidative properties. However, epidemiological studies and nutritional approaches of vitamin E treatment are controversial. Here, we investigate the effect of α-tocotrienol, which belongs to the group of vitamin E, on AD-relevant processes in neuronal cell lines. In line with the literature, α-tocotrienol reduced the ROS level in SH-SY5Y cells. In the presence of tocotrienols, cholesterol and cholesterol esters, which have been shown to be risk factors in AD, were decreased. Besides the unambiguous positive effects of tocotrienol, amyloid-β (Aβ) levels were increased accompanied by an increase in the activity of enzymes responsible for Aβ production. Proteins and gene expression of the secretases and their components remained unchanged, whereas tocotrienol accelerates enzyme activity in cell-free assays. Besides enhanced Aβ production, tocotrienols inhibited Aβ degradation in neuro 2a (N2a)-cells. Our results might help to understand the controversial findings of vitamin E studies and demonstrate that besides the known positive neuroprotective properties, tocotrienols also have negative characteristics with respect to AD. Full article

(This article belongs to the Special Issue Tocopherols and Tocotrienols: Metabolism and Properties)

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13 pages, 4113 KiB

Open AccessArticle

The Neuroprotective Properties of Hericium erinaceus in Glutamate-Damaged Differentiated PC12 Cells and an Alzheimer’s Disease Mouse Model

by Junrong Zhang¹, Shengshu An¹, Wenji Hu¹, Meiyu Teng¹, Xue Wang¹, Yidi Qu¹, Yang Liu¹, Ye Yuan² and Di Wang^1,*

¹ School of Life Sciences, Jilin University, Changchun 130012, China

² Department of Laboratory Medicine, The First Hospital of Jilin University, Changchun 130021, China

Int. J. Mol. Sci. 2016, 17(11), 1810; https://doi.org/10.3390/ijms17111810 - 1 Nov 2016

Cited by 86 | Viewed by 17184

Abstract

Hericium erinaceus, an edible and medicinal mushroom, displays various pharmacological activities in the prevention of dementia in conditions such as Parkinson’s and Alzheimer’s disease. The present study explored the neuroprotective effects of H. erinaceus mycelium polysaccharide-enriched aqueous extract (HE) on an l [...] Read more.

Hericium erinaceus, an edible and medicinal mushroom, displays various pharmacological activities in the prevention of dementia in conditions such as Parkinson’s and Alzheimer’s disease. The present study explored the neuroprotective effects of H. erinaceus mycelium polysaccharide-enriched aqueous extract (HE) on an l-glutamic acid (l-Glu)-induced differentiated PC12 (DPC12) cellular apoptosis model and an AlCl₃ combined with d-galactose-induced Alzheimer’s disease mouse model. The data revealed that HE successfully induced PC12 cell differentiation. A 3 h HE incubation at doses of 50 and 100 µg/mL before 25 mM of l-Glu effectively reversed the reduction of cell viability and the enhancement of the nuclear apoptosis rate in DPC12 cells. Compared with l-Glu-damaged cells, in PC12 cells, HE suppressed intracellular reactive oxygen species accumulation, blocked Ca²⁺ overload and prevented mitochondrial membrane potential (MMP) depolarization. In the Alzheimer’s disease mouse model, HE administration enhanced the horizontal and vertical movements in the autonomic activity test, improved the endurance time in the rotarod test, and decreased the escape latency time in the water maze test. It also improved the central cholinergic system function in the Alzheimer’s mice, demonstrated by the fact that it dose-dependently enhanced the acetylcholine (Ach) and choline acetyltransferase (ChAT) concentrations in both the serum and the hypothalamus. Our findings provide experimental evidence that HE may provide neuroprotective candidates for treating or preventing neurodegenerative diseases. Full article

(This article belongs to the Special Issue Neuroprotective Strategies 2016)

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14 pages, 10096 KiB

Open AccessArticle

Deletion of afpab1 Causes Increased Sensitivity to Oxidative Stress and Hypovirulence in Aspergillus fumigatus

by Dongyang Wang, Shunan Wang, Dan He, Song Gao, Baiji Xue and Li Wang^*

Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun 130021, China

Int. J. Mol. Sci. 2016, 17(11), 1811; https://doi.org/10.3390/ijms17111811 - 29 Oct 2016

Cited by 3 | Viewed by 4639

Abstract

Aspergillus fumigatus AFPAB1 is the ortholog of the Aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules AOPAB1 that function to depress the initiation of translation during stress. A. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research [...] Read more.

Aspergillus fumigatus AFPAB1 is the ortholog of the Aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules AOPAB1 that function to depress the initiation of translation during stress. A. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on Pab1 in A. fumigatus. The associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain Δafpab1. Phenotypic analysis showed that the Δafpab1 grew more weakly than the wild-type strain. Also the germination rate of Δafpab1 was decreased when exposed to oxidative stress. The morphology of Δafpab1 spores also showed great changes. The killing rate of Δafpab1 by RAW264.7 murine macrophage cells was increased, and the reactive oxygen species (ROS) scavenging ability of Δafpab1 was decreased. Pathogenicity testing showed that the deletion strain had decreased virulence. Therefore, we conclude that afpab1 activity is correlated with susceptibility to oxidative stress, and deletion of afpab1 from A. fumigatus possibly leads to observed hypovirulence in an immunosuppressed mouse model. Full article

(This article belongs to the Section Biochemistry)

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20 pages, 2288 KiB

Open AccessArticle

The Rheology behind Stress-Induced Solidification in Native Silk Feedstocks

by Peter R. Laity^*

and Chris Holland^*

Department of Materials Science and Engineering, The University of Sheffield, Sir Robert Hadfield Building, Mappin Street, Sheffield S1 3JD, UK

Int. J. Mol. Sci. 2016, 17(11), 1812; https://doi.org/10.3390/ijms17111812 - 29 Oct 2016

Cited by 45 | Viewed by 6855

Abstract

The mechanism by which native silk feedstocks are converted to solid fibres in nature has attracted much interest. To address this question, the present work used rheology to investigate the gelation of Bombyx mori native silk feedstock. Exceeding a critical shear stress appeared [...] Read more.

The mechanism by which native silk feedstocks are converted to solid fibres in nature has attracted much interest. To address this question, the present work used rheology to investigate the gelation of Bombyx mori native silk feedstock. Exceeding a critical shear stress appeared to be more important than shear rate, during flow-induced initiation. Compositional changes (salts, pH etc.,) were not required, although their possible role in vivo is not excluded. Moreover, after successful initiation, gel strength continued to increase over a considerable time under effectively quiescent conditions, without requiring further application of the initial stimulus. Gelation by elevated temperature or freezing was also observed. Prior to gelation, literature suggests that silk protein adopts a random coil configuration, which argued against the conventional explanation of gelation, based on hydrophilic and hydrophobic interactions. Instead, a new hypothesis is presented, based on entropically-driven loss of hydration, which appears to explain the apparently diverse methods by which silk feedstocks can be gelled. Full article

(This article belongs to the Special Issue Silk-Based Materials: From Production to Characterization)

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13 pages, 1256 KiB

Open AccessArticle

Directed Evolution of Dunaliella salina Ds-26-16 and Salt-Tolerant Response in Escherichia coli

by Yuan Guo¹, Yanping Dong¹, Xiao Hong², Xiaonan Pang², Defu Chen^2,* and Xiwen Chen^1,*

¹ Department of Biochemistry and Molecular Biology, College of Life Sciences, Nankai University, No. 94 Weijin Rd., Tianjin 300071, China

² Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, No. 94 Weijin Rd., Tianjin 300071, China

Int. J. Mol. Sci. 2016, 17(11), 1813; https://doi.org/10.3390/ijms17111813 - 29 Oct 2016

Cited by 6 | Viewed by 4957

Abstract

Identification and evolution of salt tolerant genes are crucial steps in developing salt tolerant crops or microorganisms using biotechnology. Ds-26-16, a salt tolerant gene that was isolated from Dunaliella salina, encodes a transcription factor that can confer salt tolerance to a [...] Read more.

Identification and evolution of salt tolerant genes are crucial steps in developing salt tolerant crops or microorganisms using biotechnology. Ds-26-16, a salt tolerant gene that was isolated from Dunaliella salina, encodes a transcription factor that can confer salt tolerance to a number of organisms including Escherichia coli (E. coli), Haematococcus pluvialis and tobacco. To further improve its salt tolerance, a random mutagenesis library was constructed using deoxyinosine triphosphate-mediated error-prone PCR technology, and then screened using an E. coli expression system that is based on its broad-spectrum salt tolerance. Seven variants with enhanced salt tolerance were obtained. Variant EP-5 that contained mutation S32P showed the most improvement with the E. coli transformant enduring salt concentrations up to 1.54 M, in comparison with 1.03 M for the wild type gene. Besides, Ds-26-16 and EP-5 also conferred E. coli transformant tolerance to freezing, cold, heat, Cu²⁺ and alkaline. Homology modeling revealed that mutation S32P in EP-5 caused the conformational change of N- and C-terminal α-helixes. Expression of Ds-26-16 and EP-5 maintained normal cellular morphology, increased the intracellular antioxidant enzymatic activity, reduced malondialdehyde content, and stimulated Nitric Oxide synthesis, thus enhancing salt tolerance to E. coli transformants. Full article

(This article belongs to the Section Biochemistry)

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15 pages, 3615 KiB

Open AccessArticle

Assessing Heterogeneity of Osteolytic Lesions in Multiple Myeloma by ¹H HR-MAS NMR Metabolomics

by Laurette Tavel^1,†, Francesca Fontana^2,3,†,‡, Josè Manuel Garcia Manteiga^2,†,§, Silvia Mari^1,‖, Elisabetta Mariani⁴, Enrico Caneva⁵, Roberto Sitia^2,3, Francesco Camnasio⁶, Magda Marcatti⁷, Simone Cenci^3,4,*,¶ and Giovanna Musco^1,*,¶

¹ Biomolecular NMR Unit, Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milano, Italy

² Protein Transport and Secretion Unit, Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milano, Italy

³ Università Vita-Salute San Raffaele, 20132 Milano, Italy

⁴ Age Related Diseases Unit, Division of Genetics and Cell Biology, San Raffaele Scientific Institute, 20132 Milano, Italy

⁵ Centro Interdipartimentale Grandi Apparecchiature, Università di Milano, 20132 Milano, Italy

⁶ Department of Orthopaedics and Traumatology, Orthopaedics, San Raffaele Scientific Institute, 20132 Milano, Italy

⁷ Department of Oncohematology, Hematology and Bone Marrow Transplantation Unit, San Raffaele Scientific Institute, 20132 Milano, Italy

^† These authors contributed equally to this paper.

^‡ Present address: Department of Medicine, Division of Bone and Mineral Diseases, Musculoskeletal Research Center, Washington University School of Medicine, St. Louis, MO 63110, USA

^§ Present address: Center for Translational Genomics and Bioinformatics, San Raffaele Scientific Institute, 20132 Milano, Italy

^‖ Present address: Mestrelab Research S.L., 15706 Santiago de Compostela, Spain

^¶ These corresponding authors contributed equally to this paper.

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Int. J. Mol. Sci. 2016, 17(11), 1814; https://doi.org/10.3390/ijms17111814 - 31 Oct 2016

Cited by 5 | Viewed by 5083

Abstract

Multiple myeloma (MM) is a malignancy of plasma cells characterized by multifocal osteolytic bone lesions. Macroscopic and genetic heterogeneity has been documented within MM lesions. Understanding the bases of such heterogeneity may unveil relevant features of MM pathobiology. To this aim, we deployed [...] Read more.

Multiple myeloma (MM) is a malignancy of plasma cells characterized by multifocal osteolytic bone lesions. Macroscopic and genetic heterogeneity has been documented within MM lesions. Understanding the bases of such heterogeneity may unveil relevant features of MM pathobiology. To this aim, we deployed unbiased ¹H high-resolution magic-angle spinning (HR-MAS) nuclear magnetic resonance (NMR) metabolomics to analyze multiple biopsy specimens of osteolytic lesions from one case of pathological fracture caused by MM. Multivariate analyses on normalized metabolite peak integrals allowed clusterization of samples in accordance with a posteriori histological findings. We investigated the relationship between morphological and NMR features by merging morphological data and metabolite profiling into a single correlation matrix. Data-merging addressed tissue heterogeneity, and greatly facilitated the mapping of lesions and nearby healthy tissues. Our proof-of-principle study reveals integrated metabolomics and histomorphology as a promising approach for the targeted study of osteolytic lesions. Full article

(This article belongs to the Special Issue Cellular and Molecular Mechanisms of Bone Metastasis)

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10 pages, 3021 KiB

Open AccessArticle

In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography

by Coralie Genevois

, Hugues Loiseau and Franck Couillaud^*

Molecular Imaging and Innovative Therapy in Oncology (IMOTION), EA 7435, University of Bordeaux, Bordeaux 33076, France

Int. J. Mol. Sci. 2016, 17(11), 1815; https://doi.org/10.3390/ijms17111815 - 31 Oct 2016

Cited by 25 | Viewed by 8892

Abstract

Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging [...] Read more.

Reporter gene-based strategies are widely used in experimental oncology. Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and d-luciferin as a substrate is currently the most widely employed technique. The present paper compares the performances of BLI imaging with fluorescence imaging using the near infrared fluorescent protein (iRFP) to monitor brain tumor growth in mice. Fluorescence imaging includes fluorescence reflectance imaging (FRI), fluorescence diffuse ^optical tomography (fDOT), and fluorescence molecular Imaging (FMT^®). A U87 cell line was genetically modified for constitutive expression of both the encoding Fluc and iRFP reporter genes and assayed for cell, subcutaneous tumor and brain tumor imaging. On cultured cells, BLI was more sensitive than FRI; in vivo, tumors were first detected by BLI. Fluorescence of iRFP provided convenient tools such as flux cytometry, direct detection of the fluorescent protein on histological slices, and fluorescent tomography that allowed for 3D localization and absolute quantification of the fluorescent signal in brain tumors. Full article

(This article belongs to the Special Issue Cancer Molecular Imaging in the Era of Precision Medicine)

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14 pages, 1487 KiB

Open AccessReview

The Molecular Pathway of Argon-Mediated Neuroprotection

by Felix Ulbrich and Ulrich Goebel^*

Medical Center, Department of Anesthesiology and Critical Care, Faculty of Medicine, University of Freiburg, Hugstetter Strasse 55, 79106 Freiburg im Breisgau, Germany

Int. J. Mol. Sci. 2016, 17(11), 1816; https://doi.org/10.3390/ijms17111816 - 31 Oct 2016

Cited by 35 | Viewed by 6940

Abstract

The noble gas argon has attracted increasing attention in recent years, especially because of its neuroprotective properties. In a variety of models, ranging from oxygen-glucose deprivation in cell culture to complex models of mid-cerebral artery occlusion, subarachnoid hemorrhage or retinal ischemia-reperfusion injury in [...] Read more.

The noble gas argon has attracted increasing attention in recent years, especially because of its neuroprotective properties. In a variety of models, ranging from oxygen-glucose deprivation in cell culture to complex models of mid-cerebral artery occlusion, subarachnoid hemorrhage or retinal ischemia-reperfusion injury in animals, argon administration after individual injury demonstrated favorable effects, particularly increased cell survival and even improved neuronal function. As an inert molecule, argon did not show signs of adverse effects in the in vitro and in vivo model used, while being comparably cheap and easy to apply. However, the molecular mechanism by which argon is able to exert its protective and beneficial characteristics remains unclear. Although there are many pieces missing to complete the signaling pathway throughout the cell, it is the aim of this review to summarize the known parts of the molecular pathways and to combine them to provide a clear insight into the cellular pathway, starting with the receptors that may be involved in mediating argons effects and ending with the translational response. Full article

(This article belongs to the Special Issue Neuroprotective Strategies 2016)

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16 pages, 1353 KiB

Open AccessReview

Neuroglobin, a Factor Playing for Nerve Cell Survival

by Diego Guidolin^1,*

, Cinzia Tortorella¹, Manuela Marcoli², Guido Maura² and Luigi F. Agnati^3,4

¹ Department of Neuroscience, University of Padova, Padova 35122, Italy

² Department of Pharmacy and Center of Excellence for Biomedical Research, University of Genova, Genova 16126, Italy

³ Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena 41121, Italy

⁴ Department of Neuroscience, Karolinska Institutet, Stockholm 17177, Sweden

Int. J. Mol. Sci. 2016, 17(11), 1817; https://doi.org/10.3390/ijms17111817 - 31 Oct 2016

Cited by 26 | Viewed by 8677

Abstract

Cell death represents the final outcome of several pathological conditions of the central nervous system and available evidence suggests that in both acute injuries and neurodegenerative diseases it is often associated with mitochondrial dysfunction. Thus, the possibility to prevent mitochondrial events involved in [...] Read more.

Cell death represents the final outcome of several pathological conditions of the central nervous system and available evidence suggests that in both acute injuries and neurodegenerative diseases it is often associated with mitochondrial dysfunction. Thus, the possibility to prevent mitochondrial events involved in cell death might represent efficient tools to limit neuronal damage. In recent years, increased attention has been paid to the endogenous protein neuroglobin, since accumulating evidence showed that its high expression was associated with preserved mitochondrial function and to an increased survival of nerve cells in vitro and in vivo in a variety of experimental models of cell insult. The biological and structural features of neuroglobin and the mitochondria-related mechanisms of neuroglobin-induced neuroprotection will be here briefly discussed. In this respect, the inhibition of the intrinsic pathway of apoptosis emerges as a key neuroprotective effect induced by the protein. These findings could open the possibility to develop efficient neuroglobin-mediated therapeutic strategies aimed at minimizing the neuronal cell death occurring in impacting neurological pathologies like stroke and neurodegenerative diseases. Full article

(This article belongs to the Collection Programmed Cell Death and Apoptosis)

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17 pages, 4149 KiB

Open AccessReview

Transition Metal Intercalators as Anticancer Agents—Recent Advances

by Krishant M. Deo^1,2,†, Benjamin J. Pages^1,2,†, Dale L. Ang^1,2, Christopher P. Gordon² and Janice R. Aldrich-Wright^1,2,*

¹ Nanoscale Organisation and Dynamics Group, Western Sydney University, Campbelltown, NSW 2560, Australia

² School of Science and Health, Western Sydney University, Campbelltown, NSW 2560, Australia

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1818; https://doi.org/10.3390/ijms17111818 - 31 Oct 2016

Cited by 78 | Viewed by 10864

Abstract

The diverse anticancer utility of cisplatin has stimulated significant interest in the development of additional platinum-based therapies, resulting in several analogues receiving clinical approval worldwide. However, due to structural and mechanistic similarities, the effectiveness of platinum-based therapies is countered by severe side-effects, narrow [...] Read more.

The diverse anticancer utility of cisplatin has stimulated significant interest in the development of additional platinum-based therapies, resulting in several analogues receiving clinical approval worldwide. However, due to structural and mechanistic similarities, the effectiveness of platinum-based therapies is countered by severe side-effects, narrow spectrum of activity and the development of resistance. Nonetheless, metal complexes offer unique characteristics and exceptional versatility, with the ability to alter their pharmacology through facile modifications of geometry and coordination number. This has prompted the search for metal-based complexes with distinctly different structural motifs and non-covalent modes of binding with a primary aim of circumventing current clinical limitations. This review discusses recent advances in platinum and other transition metal-based complexes with mechanisms of action involving intercalation. This mode of DNA binding is distinct from cisplatin and its derivatives. The metals focused on in this review include Pt, Ru and Cu along with examples of Au, Ni, Zn and Fe complexes; these complexes are capable of DNA intercalation and are highly biologically active. Full article

(This article belongs to the Special Issue Recent Advances in Metal Based Drugs)

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11 pages, 2234 KiB

Open AccessArticle

Cx43 Mediates Resistance against MPP⁺-Induced Apoptosis in SH-SY5Y Neuroblastoma Cells via Modulating the Mitochondrial Apoptosis Pathway

by In-Su Kim¹

, Palanivel Ganesan² and Dong-Kug Choi^1,*

¹ Department of Biotechnology, Konkuk University, Chungju 380-701, Korea

² Nanotechnology Research Center and Department of Applied Life Science, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea

Int. J. Mol. Sci. 2016, 17(11), 1819; https://doi.org/10.3390/ijms17111819 - 1 Nov 2016

Cited by 22 | Viewed by 8342

Abstract

Neuronal apoptosis in the substantia nigra par compacta (SNpc) appears to play an essential role in the pathogenesis of Parkinson’s disease. However, the mechanisms responsible for the death of dopaminergic neurons are not fully understood yet. To explore the apoptotic mechanisms, we used [...] Read more.

Neuronal apoptosis in the substantia nigra par compacta (SNpc) appears to play an essential role in the pathogenesis of Parkinson’s disease. However, the mechanisms responsible for the death of dopaminergic neurons are not fully understood yet. To explore the apoptotic mechanisms, we used a well-known parkinsonian toxin, 1-methyl-4-phenylpyridine (MPP⁺), to induce neuronal apoptosis in the human dopaminergic SH-SY5Y cell line. The most common method of interaction between cells is gap junctional intercellular communication (GJIC) mediated by gap junctions (GJs) formed by transmembrane proteins called connexins (Cx). Modulation of GJIC affects cell viability or growth, implying that GJIC may have an important role in maintaining homeostasis in various organs. Here, we hypothesized that increasing the level of the gap junction protein Cx43 in SH-SY5Y neuroblastoma cells could provide neuroprotection. First, our experiments demonstrated that knocking down Cx43 protein by using Cx43-specific shRNA in SH-SY5Y neuroblastoma cells potentiated MPP⁺-induced neuronal apoptosis evident from decreased cell viability. In another experiment, we demonstrated that over-expression of Cx43 in the SH-SY5Y cell system decreased MPP⁺-induced apoptosis based on the MTT assay and reduced the Bax/Bcl-2 ratio and the release of cytochrome C based on Western blot analysis. Taken together, our results suggest that Cx43 could mediate resistance against MPP⁺-induced apoptosis in SH-SY5Y neuroblastoma cells via modulating the mitochondrial apoptosis pathway. Full article

(This article belongs to the Collection Programmed Cell Death and Apoptosis)

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15 pages, 4709 KiB

Open AccessArticle

Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS

by Xiaochen Liu¹

, Deyi Lu¹, Robert Bowser² and Jian Liu^1,*

¹ Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou 215123, China

² Department of Neurobiology, Barrow Neurological Institute, Phoenix, AZ 85013, USA

Int. J. Mol. Sci. 2016, 17(11), 1820; https://doi.org/10.3390/ijms17111820 - 1 Nov 2016

Cited by 19 | Viewed by 6044

Abstract

Carbonic anhydrase I (CA1) is the cytosolic isoform of mammalian α-CA family members which are responsible for maintaining pH homeostasis in the physiology and pathology of organisms. A subset of CA isoforms are known to be expressed and function in the central nervous [...] Read more.

Carbonic anhydrase I (CA1) is the cytosolic isoform of mammalian α-CA family members which are responsible for maintaining pH homeostasis in the physiology and pathology of organisms. A subset of CA isoforms are known to be expressed and function in the central nervous system (CNS). CA1 has not been extensively characterized in the CNS. In this study, we demonstrate that CA1 is expressed in the motor neurons in human spinal cord. Unexpectedly, a subpopulation of CA1 appears to be associated with endoplasmic reticulum (ER) membranes. In addition, the membrane-associated CA1s are preferentially upregulated in amyotrophic lateral sclerosis (ALS) and exhibit altered distribution in motor neurons. Furthermore, long-term expression of CA1 in mammalian cells activates apoptosis. Our results suggest a previously unknown role for CA1 function in the CNS and its potential involvement in motor neuron degeneration in ALS. Full article

(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)

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13 pages, 2588 KiB

Open AccessArticle

Assessment of Photodynamic Inactivation against Periodontal Bacteria Mediated by a Chitosan Hydrogel in a 3D Gingival Model

by Po-Chun Peng^1,†, Chien-Ming Hsieh^2,†, Chueh-Pin Chen¹, Tsuimin Tsai³ and Chin-Tin Chen^1,*

¹ Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan

² Department of Cosmetic Science, Providence University, Taichung City 43301, Taiwan

³ Graduate Institute of Biomedical Materials and Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei 11031, Taiwan

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1821; https://doi.org/10.3390/ijms17111821 - 1 Nov 2016

Cited by 30 | Viewed by 6080

Abstract

Chitosan hydrogels containing hydroxypropyl methylcellulose (HPMC) and toluidine blue O were prepared and assessed for their mucoadhesive property and antimicrobial efficacy of photodynamic inactivation (PDI). Increased HPMC content in the hydrogels resulted in increased mucoadhesiveness. Furthermore, we developed a simple In Vitro 3D [...] Read more.

Chitosan hydrogels containing hydroxypropyl methylcellulose (HPMC) and toluidine blue O were prepared and assessed for their mucoadhesive property and antimicrobial efficacy of photodynamic inactivation (PDI). Increased HPMC content in the hydrogels resulted in increased mucoadhesiveness. Furthermore, we developed a simple In Vitro 3D gingival model resembling the oral periodontal pocket to culture the biofilms of Staphylococcus aureus (S. aureus), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), and Porphyromonas gingivalis (P. gingivalis). The PDI efficacy of chitosan hydrogel was examined against periodontal biofilms cultured in this 3D gingival model. We found that the PDI effectiveness was limited due to leaving some of the innermost bacteria alive at the non-illuminated site. Using this 3D gingival model, we further optimized PDI procedures with various adjustments of light energy and irradiation sites. The PDI efficacy of the chitosan hydrogel against periodontal biofilms can significantly improve via four sides of irradiation. In conclusion, this study not only showed the clinical applicability of this chitosan hydrogel but also the importance of the light irradiation pattern in performing PDI for periodontal disease. Full article

(This article belongs to the Special Issue Antimicrobial Polymers 2016)

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26 pages, 1298 KiB

Open AccessReview

Extracellular Matrix, a Hard Player in Angiogenesis

by Maurizio Mongiat^*,†

, Eva Andreuzzi^†

, Giulia Tarticchio and Alice Paulitti

¹ Experimental Oncology Division 2, Department of Translational Research, CRO-IRCCS, Aviano 33081, Italy

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1822; https://doi.org/10.3390/ijms17111822 - 1 Nov 2016

Cited by 180 | Viewed by 10773

Abstract

The extracellular matrix (ECM) is a complex network of proteins, glycoproteins, proteoglycans, and polysaccharides. Through multiple interactions with each other and the cell surface receptors, not only the ECM determines the physical and mechanical properties of the tissues, but also profoundly influences cell [...] Read more.

The extracellular matrix (ECM) is a complex network of proteins, glycoproteins, proteoglycans, and polysaccharides. Through multiple interactions with each other and the cell surface receptors, not only the ECM determines the physical and mechanical properties of the tissues, but also profoundly influences cell behavior and many physiological and pathological processes. One of the functions that have been extensively explored is its impingement on angiogenesis. The strong impact of the ECM in this context is both direct and indirect by virtue of its ability to interact and/or store several growth factors and cytokines. The aim of this review is to provide some examples of the complex molecular mechanisms that are elicited by these molecules in promoting or weakening the angiogenic processes. The scenario is intricate, since matrix remodeling often generates fragments displaying opposite effects compared to those exerted by the whole molecules. Thus, the balance will tilt towards angiogenesis or angiostasis depending on the relative expression of pro- or anti-angiogenetic molecules/fragments composing the matrix of a given tissue. One of the vital aspects of this field of research is that, for its endogenous nature, the ECM can be viewed as a reservoir to draw from for the development of new more efficacious therapies to treat angiogenesis-dependent pathologies. Full article

(This article belongs to the Special Issue Vascular Biology and Therapeutics)

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16 pages, 590 KiB

Open AccessArticle

Pretransplant Levels of CRP and Interleukin-6 Family Cytokines; Effects on Outcome after Allogeneic Stem Cell Transplantation

by Tor Henrik Tvedt^1,*, Stein Atle Lie², Håkon Reikvam^1,3

, Kristin Paulsen Rye³, Roald Lindås¹, Tobias Gedde-Dahl⁴, Aymen Bushra Ahmed¹ and Øystein Bruserud^1,3

¹ Section for Hematology, Department of Medicine, Haukeland University Hospital, 5021 Bergen, Norway

² Department of Clinical Dentistry, University of Bergen, 5020 Bergen, Norway

³ Section for Hematology, Institute of Clinical Science, University of Bergen, 5020 Bergen, Norway

⁴ Department of Hematology, University of Oslo, 0424 Oslo, Norway

Int. J. Mol. Sci. 2016, 17(11), 1823; https://doi.org/10.3390/ijms17111823 - 1 Nov 2016

Cited by 31 | Viewed by 5857

Abstract

Several pretransplant factors, including CRP (C-reactive protein) levels, reflect the risk of complications after allogeneic stem cell transplantation. IL-6 induces CRP increase, and we therefore investigated the effects of pretransplant IL-6, soluble IL-6 receptors, IL-6 family cytokines and CRP serum levels on outcome [...] Read more.

Several pretransplant factors, including CRP (C-reactive protein) levels, reflect the risk of complications after allogeneic stem cell transplantation. IL-6 induces CRP increase, and we therefore investigated the effects of pretransplant IL-6, soluble IL-6 receptors, IL-6 family cytokines and CRP serum levels on outcome for 100 consecutive allotransplant recipients. All patients had related donors, none had active infections and 99 patients were in complete remission before conditioning. The incidence of acute graft versus host disease (aGVHD) requiring treatment was 40%, survival at Day +100 82%, and overall survival 48%. Despite a significant correlation between pretransplant CRP and IL-6 levels, only CRP levels significantly influenced transplant-related mortality (TRM). However, CRP did not influence overall survival (OS). Pretransplant IL-31 influenced late TRM. Finally, there was a significant association between pretransplant IL-6 and early postconditioning weight gain (i.e., fluid retention), and this fluid retention was a risk factor for aGVHD, TRM and OS. To conclude, pretransplant CRP, IL-31 and early posttransplant fluid retention were independent risk factors for TRM and survival after allotransplantation. Full article

(This article belongs to the Special Issue Advances in Cell Transplantation)

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13 pages, 4048 KiB

Open AccessArticle

Effect of High-Carbohydrate Diet on Plasma Metabolome in Mice with Mitochondrial Respiratory Chain Complex III Deficiency

by Jayasimman Rajendran^1,2

, Nikica Tomašić^3,4, Heike Kotarsky³, Eva Hansson³, Vidya Velagapudi⁵, Jukka Kallijärvi¹

and Vineta Fellman^1,3,6,*

¹ Folkhälsan Institute of Genetics, Folkhälsan Research Center, 00014 Helsinki, Finland

² Institute of Clinical Medicine, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland

³ Department of Clinical Sciences, Lund, Pediatrics, Lund University, 22185 Lund, Sweden

⁴ Department of Neonatology, Karolinska University Hospital, 17176 Solna, Sweden

⁵ Finnish Institute of Molecular Medicine, University of Helsinki, 00290 Helsinki, Finland

⁶ Institute of Clinical medicine, Children’s Hospital, Helsinki University Hospital and University of Helsinki, 00029 Helsinki, Finland

Int. J. Mol. Sci. 2016, 17(11), 1824; https://doi.org/10.3390/ijms17111824 - 1 Nov 2016

Cited by 12 | Viewed by 6603

Abstract

Mitochondrial disorders cause energy failure and metabolic derangements. Metabolome profiling in patients and animal models may identify affected metabolic pathways and reveal new biomarkers of disease progression. Using liver metabolomics we have shown a starvation-like condition in a knock-in (Bcs1l^c.232A>G) [...] Read more.

Mitochondrial disorders cause energy failure and metabolic derangements. Metabolome profiling in patients and animal models may identify affected metabolic pathways and reveal new biomarkers of disease progression. Using liver metabolomics we have shown a starvation-like condition in a knock-in (Bcs1l^c.232A>G) mouse model of GRACILE syndrome, a neonatal lethal respiratory chain complex III dysfunction with hepatopathy. Here, we hypothesized that a high-carbohydrate diet (HCD, 60% dextrose) will alleviate the hypoglycemia and promote survival of the sick mice. However, when fed HCD the homozygotes had shorter survival (mean ± SD, 29 ± 2.5 days, n = 21) than those on standard diet (33 ± 3.8 days, n = 30), and no improvement in hypoglycemia or liver glycogen depletion. We investigated the plasma metabolome of the HCD- and control diet-fed mice and found that several amino acids and urea cycle intermediates were increased, and arginine, carnitines, succinate, and purine catabolites decreased in the homozygotes. Despite reduced survival the increase in aromatic amino acids, an indicator of liver mitochondrial dysfunction, was normalized on HCD. Quantitative enrichment analysis revealed that glycine, serine and threonine metabolism, phenylalanine and tyrosine metabolism, and urea cycle were also partly normalized on HCD. This dietary intervention revealed an unexpected adverse effect of high-glucose diet in complex III deficiency, and suggests that plasma metabolomics is a valuable tool in evaluation of therapies in mitochondrial disorders. Full article

(This article belongs to the Special Issue Metabolomic Technologies in Medicine)

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23 pages, 6538 KiB

Open AccessArticle

MMP-3 Deficiency Alleviates Endotoxin-Induced Acute Inflammation in the Posterior Eye Segment

by Inge Van Hove^1,2,†

, Evy Lefevere^1,2,†, Lies De Groef^1,2

, Jurgen Sergeys^1,2

, Manuel Salinas-Navarro¹, Claude Libert^3,4, Roosmarijn Vandenbroucke^3,4

and Lieve Moons^1,*

¹ Neural Circuit Development and Regeneration Research Group, Department of Biology, Katholieke Universiteit Leuven (KU Leuven), B-3000 Leuven, Belgium

² Laboratory of Experimental Ophthalmology, Department of Neurosciences, KU Leuven, B-3000 Leuven, Belgium

³ Inflammation Research Center, VIB, B-9052 Ghent, Belgium

⁴ Department of Biomedical Molecular Biology, Ghent University, B-9052 Ghent, Belgium

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1825; https://doi.org/10.3390/ijms17111825 - 1 Nov 2016

Cited by 32 | Viewed by 8360

Abstract

Matrix metalloproteinase-3 (MMP-3) is known to mediate neuroinflammatory processes by activating microglia, disrupting blood–central nervous system barriers and supporting neutrophil influx into the brain. In addition, the posterior part of the eye, more specifically the retina, the retinal pigment epithelium (RPE) and the [...] Read more.

Matrix metalloproteinase-3 (MMP-3) is known to mediate neuroinflammatory processes by activating microglia, disrupting blood–central nervous system barriers and supporting neutrophil influx into the brain. In addition, the posterior part of the eye, more specifically the retina, the retinal pigment epithelium (RPE) and the blood–retinal barrier, is affected upon neuroinflammation, but a role for MMP-3 during ocular inflammation remains elusive. We investigated whether MMP-3 contributes to acute inflammation in the eye using the endotoxin-induced uveitis (EIU) model. Systemic administration of lipopolysaccharide induced an increase in MMP-3 mRNA and protein expression level in the posterior part of the eye. MMP-3 deficiency or knockdown suppressed retinal leukocyte adhesion and leukocyte infiltration into the vitreous cavity in mice subjected to EIU. Moreover, retinal and RPE mRNA levels of intercellular adhesion molecule 1 (Icam1), interleukin 6 (Il6), cytokine-inducible nitrogen oxide synthase (Nos2) and tumor necrosis factor α (Tnfα), which are key molecules involved in EIU, were clearly reduced in MMP-3 deficient mice. In addition, loss of MMP-3 repressed the upregulation of the chemokines monocyte chemoattractant protein (MCP)-1 and (C-X-C motif) ligand 1 (CXCL1). These findings suggest a contribution of MMP-3 during EIU, and its potential use as a therapeutic drug target in reducing ocular inflammation. Full article

(This article belongs to the Special Issue Metalloproteins 2017)

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10 pages, 715 KiB

Open AccessReview

Role of Oxidative Stress in Drug-Induced Kidney Injury

by Keiko Hosohata

Education and Reseearch Center for Clinical Pharmacy, Osaka University of Pharmaceutical Sciences, Osaka 569-1094, Japan

Int. J. Mol. Sci. 2016, 17(11), 1826; https://doi.org/10.3390/ijms17111826 - 1 Nov 2016

Cited by 163 | Viewed by 11037

Abstract

The kidney plays a primary role in maintaining homeostasis and detoxification of numerous hydrophilic xenobiotics as well as endogenous compounds. Because the kidney is exposed to a larger proportion and higher concentration of drugs and toxins than other organs through the secretion of [...] Read more.

The kidney plays a primary role in maintaining homeostasis and detoxification of numerous hydrophilic xenobiotics as well as endogenous compounds. Because the kidney is exposed to a larger proportion and higher concentration of drugs and toxins than other organs through the secretion of ionic drugs by tubular organic ion transporters across the luminal membranes of renal tubular epithelial cells, and through the reabsorption of filtered toxins into the lumen of the tubule, these cells are at greater risk for injury. In fact, drug-induced kidney injury is a serious problem in clinical practice and accounts for roughly 20% of cases of acute kidney injury (AKI) among hospitalized patients. Therefore, its early detection is becoming more important. Usually, drug-induced AKI consists of two patterns of renal injury: acute tubular necrosis (ATN) and acute interstitial nephritis (AIN). Whereas AIN develops from medications that incite an allergic reaction, ATN develops from direct toxicity on tubular epithelial cells. Among several cellular mechanisms underlying ATN, oxidative stress plays an important role in progression to ATN by activation of inflammatory response via proinflammatory cytokine release and inflammatory cell accumulation in tissues. This review provides an overview of drugs associated with AKI, the role of oxidative stress in drug-induced AKI, and a biomarker for drug-induced AKI focusing on oxidative stress. Full article

(This article belongs to the Special Issue Biomarkers in Drug-Induced Organ Injury)

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16 pages, 3416 KiB

Open AccessArticle

The Effect of Everolimus in an In Vitro Model of Triple Negative Breast Cancer and Osteoclasts

by Laura Mercatali^*,†, Chiara Spadazzi^†, Giacomo Miserocchi, Chiara Liverani, Alessandro De Vita

, Alberto Bongiovanni

, Federica Recine, Dino Amadori and Toni Ibrahim

¹ Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, Italy

^† The authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1827; https://doi.org/10.3390/ijms17111827 - 1 Nov 2016

Cited by 32 | Viewed by 5451

Abstract

Metastatic bone disease has a major impact on morbidity of breast cancer (BC) patients. Alterations in mTOR signaling are involved both in cancer progression and in osteoclast differentiation. The purpose of this study was to assess the role of mTOR inhibitor Everolimus (Eve) [...] Read more.

Metastatic bone disease has a major impact on morbidity of breast cancer (BC) patients. Alterations in mTOR signaling are involved both in cancer progression and in osteoclast differentiation. The purpose of this study was to assess the role of mTOR inhibitor Everolimus (Eve) on osteoclastogenesis induced by triple negative BC cells. To this aim, we developed an in vitro human model of osteoclastogenesis from peripheral blood monocytes co-cultured with the triple negative SCP2 and the hormonal receptor positive MCF7 cell lines. Osteoclastogenesis was evaluated by TRAP staining, evaluation of F actin rings and Calcitonin Receptor expression. Eve significantly reduced differentiation induced by cancer cells and resulted more effective when evaluated in combination with Denosumab and Zoledronic Acid (Zol). Combination with Zol showed a total abrogation of osteoclast differentiation induced by the triple negative cell line, not by MCF7. Finally, we observed that Eve was active in the inhibition of the crosstalk between cancer cells and osteoclasts reproduced by our model, highlighting a new therapeutic choice for the subsetting of triple negative BC patients. We observed a difference in the response to bone-targeted therapy with respect to BC subtypes. Our model may represent a valid platform for preclinical trials on bone-targeted drugs and for the study of the interplay of BC with bone stromal cells. Full article

(This article belongs to the Special Issue Cellular and Molecular Mechanisms of Bone Metastasis)

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13 pages, 1124 KiB

Open AccessArticle

The Compositional HJ-Biplot—A New Approach to Identifying the Links among Bioactive Compounds of Tomatoes

by Marcos Hernández Suárez¹, Daniel Molina Pérez², Elena M. Rodríguez-Rodríguez², Carlos Díaz Romero², Francisco Espinosa Borreguero^3,* and Purificación Galindo-Villardón^4,5

¹ Aula Dei Scientific Technological Park Foundation, Av. Montañana 930, 50059 Zaragoza, Spain

² Department of Chemical Engineering and Pharmaceutical Technology, Food Science and Nutrition Area, University of La Laguna, 38206 La Laguna, Santa Cruz de Tenerife, Spain

³ Plant Biology, Ecology and Earth Sciences Department, Extremadura University, Av. Elvas s/n, 06071 Badajoz, Spain

⁴ Statistics Department, University of Salamanca, c/Alfonso X El Sabio s/n, 37008 Salamanca, Spain

⁵ Higher Polytechnic School of the Litoral, Campus Gustavo Galindo, 09-01-5863 Guayaquil, Ecuador

Int. J. Mol. Sci. 2016, 17(11), 1828; https://doi.org/10.3390/ijms17111828 - 2 Nov 2016

Cited by 15 | Viewed by 5959

Abstract

Tomatoes have been described as a functional food because of their particular composition of different bioactive compounds. In this study, the proximate composition, minerals and trace elements, and antioxidant compounds were determined in two tomato cultivars (Mariana and Dunkan) that were grown in [...] Read more.

Tomatoes have been described as a functional food because of their particular composition of different bioactive compounds. In this study, the proximate composition, minerals and trace elements, and antioxidant compounds were determined in two tomato cultivars (Mariana and Dunkan) that were grown in Gran Canaria (Spain) either conventionally or hydroponically. Although compositional data of this type require being subjected to the specific statistical techniques of compositional analysis, this approach has not usually been considered in this context. In the present case, a compositional Mann–Whitney U test of the data showed significant differences for each factor (cultivar and cultivation system) in several of the compositional variables studied. For the differences between cultivars, these parameters were the protein, Mg, lycopene, ascorbic acid, citric acid, and fumaric acid contents. For the differences between cultivation systems, they were mainly those of the mineral and trace elements group. Although one-year data are insufficient to make clear relationship among compounds because more repetitions in several localities and years are necessary, the compositional HJ-biplot (in which the links provide estimates of the linear relationship among variables) results agreed with other scientific results about linear relationship among some compounds analyzed. Full article

(This article belongs to the Special Issue Bioactive Phytochemicals and Functional Food Ingredients in Fruits and Vegetables 2016)

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9 pages, 2089 KiB

Open AccessArticle

USP19-Mediated Deubiquitination Facilitates the Stabilization of HRD1 Ubiquitin Ligase

by Kumi Harada^†, Masako Kato^† and Nobuhiro Nakamura^*

¹ Department of Life Science and Technology, Tokyo Institute of Technology, 4259-B13 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1829; https://doi.org/10.3390/ijms17111829 - 2 Nov 2016

Cited by 23 | Viewed by 6689

Abstract

In the endoplasmic reticulum (ER), misfolded and unfolded proteins are eliminated by a process called ER-associated protein degradation (ERAD) in order to maintain cell homeostasis. In the ERAD pathway, several ER-localized E3 ubiquitin ligases target ERAD substrate proteins for ubiquitination and subsequent proteasomal [...] Read more.

In the endoplasmic reticulum (ER), misfolded and unfolded proteins are eliminated by a process called ER-associated protein degradation (ERAD) in order to maintain cell homeostasis. In the ERAD pathway, several ER-localized E3 ubiquitin ligases target ERAD substrate proteins for ubiquitination and subsequent proteasomal degradation. However, little is known about how the functions of the ERAD ubiquitin ligases are regulated. Recently, USP19, an ER-anchored deubiquitinating enzyme (DUB), has been suggested to be involved in the regulation of ERAD. In this study, HRD1, an ERAD ubiquitin ligase, is shown to be a novel substrate for USP19. We demonstrate that USP19 rescues HRD1 from proteasomal degradation by deubiquitination of K48-linked ubiquitin chains. In addition, the altered expression of USP19 affects the steady-state levels of HRD1. These results suggest that USP19 regulates the stability of HRD1 and provide insight into the regulatory mechanism of the ERAD ubiquitin ligases. Full article

(This article belongs to the Section Biochemistry)

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22 pages, 6538 KiB

Open AccessArticle

Programmed Effects in Neurobehavior and Antioxidative Physiology in Zebrafish Embryonically Exposed to Cadmium: Observations and Hypothesized Adverse Outcome Pathway Framework

by Sander Ruiter¹, Josefine Sippel¹, Manon C. Bouwmeester¹, Tobias Lommelaars¹, Piet Beekhof¹, Hennie M. Hodemaekers¹, Frank Bakker¹, Evert-Jan Van den Brandhof², Jeroen L. A. Pennings¹ and Leo T. M. Van der Ven^1,*

¹ Centre for Health Protection, National Institute for Public Health and the Environment (RIVM), Bilthoven 3720BA-1, The Netherlands

² Centre for Environmental Quality, National Institute for Public Health and the Environment (RIVM), Bilthoven 3720BA-1, The Netherlands

Int. J. Mol. Sci. 2016, 17(11), 1830; https://doi.org/10.3390/ijms17111830 - 2 Nov 2016

Cited by 25 | Viewed by 5833

Abstract

Non-communicable diseases (NCDs) are a major cause of premature mortality. Recent studies show that predispositions for NCDs may arise from early-life exposure to low concentrations of environmental contaminants. This developmental origins of health and disease (DOHaD) paradigm suggests that programming of an embryo [...] Read more.

Non-communicable diseases (NCDs) are a major cause of premature mortality. Recent studies show that predispositions for NCDs may arise from early-life exposure to low concentrations of environmental contaminants. This developmental origins of health and disease (DOHaD) paradigm suggests that programming of an embryo can be disrupted, changing the homeostatic set point of biological functions. Epigenetic alterations are a possible underlying mechanism. Here, we investigated the DOHaD paradigm by exposing zebrafish to subtoxic concentrations of the ubiquitous contaminant cadmium during embryogenesis, followed by growth under normal conditions. Prolonged behavioral responses to physical stress and altered antioxidative physiology were observed approximately ten weeks after termination of embryonal exposure, at concentrations that were 50–3200-fold below the direct embryotoxic concentration, and interpreted as altered developmental programming. Literature was explored for possible mechanistic pathways that link embryonic subtoxic cadmium to the observed apical phenotypes, more specifically, the probability of molecular mechanisms induced by cadmium exposure leading to altered DNA methylation and subsequently to the observed apical phenotypes. This was done using the adverse outcome pathway model framework, and assessing key event relationship plausibility by tailored Bradford-Hill analysis. Thus, cadmium interaction with thiols appeared to be the major contributor to late-life effects. Cadmium-thiol interactions may lead to depletion of the methyl donor S-adenosyl-methionine, resulting in methylome alterations, and may, additionally, result in oxidative stress, which may lead to DNA oxidation, and subsequently altered DNA methyltransferase activity. In this way, DNA methylation may be affected at a critical developmental stage, causing the observed apical phenotypes. Full article

(This article belongs to the Special Issue Zebrafish: A Model for Toxicological Research)

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12 pages, 3401 KiB

Open AccessArticle

by Noriko Uesugi^1,*, Yoshihito Shimazu², Kazunori Kikuchi³ and Michio Nagata¹

¹ Department of Kidney and Vascular Pathology, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8571, Japan

² Laboratory of Food and Physiological Sciences, Azabu University, Sagamihara 252-5201, Japan

³ Division of Pathology, Tsukuba Medical Center, Tsukuba 305-0005, Japan

Int. J. Mol. Sci. 2016, 17(11), 1831; https://doi.org/10.3390/ijms17111831 - 2 Nov 2016

Cited by 14 | Viewed by 5904

Abstract

The renal microvasculature is targeted during aging, sometimes producing chronic kidney disease (CKD). Overdiagnosis of CKD in older persons is concerning. To prevent it, a new concept of “healthy aging” is arising from a healthy renal donor study. We investigated the renal microcirculatory [...] Read more.

The renal microvasculature is targeted during aging, sometimes producing chronic kidney disease (CKD). Overdiagnosis of CKD in older persons is concerning. To prevent it, a new concept of “healthy aging” is arising from a healthy renal donor study. We investigated the renal microcirculatory changes of three older persons and compared them with that of one patient with nephrosclerosis using a three-dimensional (3D) reconstruction technique that we previously developed. This method uses a virtual slide system and paraffin-embedded serial sections of surgical material that was double-immunostained by anti-CD34 and anti-α smooth muscle actin (SMA) antibodies for detecting endothelial cells and medial smooth muscle cells, respectively. In all cases, the 3D images proved that arteriosclerotic changes in large proximal interlobular arteries did not directly induce distal arterial change or glomerulosclerosis. The nephrosclerotic patient showed severe hyalinosis with luminal narrowing of small arteries directly inducing glomerulosclerosis. We also visualized an atubular glomerulus and intraglomerular dilatation of an afferent arteriole during healthy aging on the 3D image and showed that microcirculatory changes were responsible for them. Thus, we successfully visualized healthy aged kidneys on 3D images and confirmed the underlying pathology. This method has the ability to investigate renal microcirculatory damage during healthy aging. Full article

(This article belongs to the Special Issue Atherosclerosis and Vascular Imaging 2016)

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13 pages, 3633 KiB

Open AccessArticle

Interferon α Induces the Apoptosis of Cervical Cancer HeLa Cells by Activating both the Intrinsic Mitochondrial Pathway and Endoplasmic Reticulum Stress-Induced Pathway

by Wei-Ye Shi¹, Cheng Cao² and Li Liu^1,*

¹ Department of Microbiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China

² Beijing Institute of Biotechnology, Academy of Military Medical Sciences, 27 Taiping Rd., Haidian District, Beijing 100850, China

Int. J. Mol. Sci. 2016, 17(11), 1832; https://doi.org/10.3390/ijms17111832 - 2 Nov 2016

Cited by 30 | Viewed by 9221

Abstract

The interferon α (IFN-α) has been often used as a sensitizing agent for the treatment of various malignancies such as hepatocellular carcinoma, malignant melanoma, and renal cell cancer by promoting the apoptosis of thesetumor cell types. However, the effect of IFN-α on cervical [...] Read more.

The interferon α (IFN-α) has been often used as a sensitizing agent for the treatment of various malignancies such as hepatocellular carcinoma, malignant melanoma, and renal cell cancer by promoting the apoptosis of thesetumor cell types. However, the effect of IFN-α on cervical cancer remains unknown. In this study, HeLa cells were used as a testing model for the treatment of IFN-α on cervical cancer. The results indicate that IFN-α markedly inhibits the proliferation and induces the apoptosis of HeLa cells. The activation of caspase 3, the up-regulation of both Bim and cleaved poly (ADP-ribose) polymerase (PARP) 1, the down-regulation of Bcl-xL, as well as the release of cytochrome c from mitochondria were significantly induced upon IFN-α treatment, indicating that the intrinsic apoptotic pathway could be activated by IFN-α treatment. In addition, caspase 4—which is involved in the endoplasmic reticulum (ER) stress-induced apoptosis—was activated in response to IFN-α treatment. Knocking down caspase 4 by small interfering RNA (siRNA) markedly reduced the IFN-α-mediated cell apoptosis. However, no significant changes in the expressions of caspases 8 and 10 were observed upon IFN-α treatment, indicating that the apoptosis caused by IFN-α might be independent of the extrinsic apoptotic pathway. These findings suggest that IFN-α may possess anti-cervical cancer capacity by activating cell apoptosis via the intrinsic mitochondrial pathway and caspase-4-related ER stress-induced pathway. Full article

(This article belongs to the Collection Programmed Cell Death and Apoptosis)

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24 pages, 2076 KiB

Open AccessArticle

Comparative Analysis of Toxic Responses of Organic Extracts from Diesel and Selected Alternative Fuels Engine Emissions in Human Lung BEAS-2B Cells

by Helena Libalova¹, Pavel Rossner,, Jr.¹, Kristyna Vrbova¹, Tana Brzicova^1,2, Jitka Sikorova^1,3, Michal Vojtisek-Lom⁴, Vit Beranek⁴, Jiri Klema⁵, Miroslav Ciganek⁶, Jiri Neca⁶, Katerina Pencikova⁶, Miroslav Machala⁶ and Jan Topinka^1,*

¹ Department of Genetic Toxicology and Nanotoxicology, Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Prague, Czech Republic

² Faculty of Safety Engineering, VSB–Technical University of Ostrava, Lumirova 13, 700 30 Ostrava, Czech Republic

³ Institute for Environmental Studies, Faculty of Science, Charles University in Prague, Benatska 2, 128 01 Prague 2, Czech Republic

⁴ Center of Vehicles for Sustainable Mobility, Faculty of Mechanical Engineering, Czech Technical University in Prague, Technicka 4, 166 07 Prague, Czech Republic

⁵ Department of Cybernetics, Faculty of Electrical Engineering, Czech Technical University in Prague, Karlovo namesti 13, 121 35 Prague, Czech Republic

⁶ Department of Chemistry and Toxicology, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic

Int. J. Mol. Sci. 2016, 17(11), 1833; https://doi.org/10.3390/ijms17111833 - 3 Nov 2016

Cited by 33 | Viewed by 7967

Abstract

This study used toxicogenomics to identify the complex biological response of human lung BEAS-2B cells treated with organic components of particulate matter in the exhaust of a diesel engine. First, we characterized particles from standard diesel (B0), biodiesel (methylesters of rapeseed oil) in [...] Read more.

This study used toxicogenomics to identify the complex biological response of human lung BEAS-2B cells treated with organic components of particulate matter in the exhaust of a diesel engine. First, we characterized particles from standard diesel (B0), biodiesel (methylesters of rapeseed oil) in its neat form (B100) and 30% by volume blend with diesel fuel (B30), and neat hydrotreated vegetable oil (NEXBTL100). The concentration of polycyclic aromatic hydrocarbons (PAHs) and their derivatives in organic extracts was the lowest for NEXBTL100 and higher for biodiesel. We further analyzed global gene expression changes in BEAS-2B cells following 4 h and 24 h treatment with extracts. The concentrations of 50 µg extract/mL induced a similar molecular response. The common processes induced after 4 h treatment included antioxidant defense, metabolism of xenobiotics and lipids, suppression of pro-apoptotic stimuli, or induction of plasminogen activating cascade; 24 h treatment affected fewer processes, particularly those involved in detoxification of xenobiotics, including PAHs. The majority of distinctively deregulated genes detected after both 4 h and 24 h treatment were induced by NEXBTL100; the deregulated genes included, e.g., those involved in antioxidant defense and cell cycle regulation and proliferation. B100 extract, with the highest PAH concentrations, additionally affected several cell cycle regulatory genes and p38 signaling. Full article

(This article belongs to the Special Issue Molecular Research on Global Climate Change and Atmospheric Pollution)

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12 pages, 948 KiB

Open AccessArticle

Factors Affecting the Baseline and Post-Treatment Scores on the Hopkins Verbal Learning Test-Revised Japanese Version before and after Whole-Brain Radiation Therapy

by Hirotake Saito^1,*, Kensuke Tanaka¹, Ayae Kanemoto², Toshimichi Nakano¹, Eisuke Abe¹

and Hidefumi Aoyama^1,*

¹ Department of Radiology and Radiation Oncology, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Chuo-ku, Niigata 951-8510, Japan

² Department of Radiation Oncology, Niigata Cancer Center Hospital, 2-15-3 Kawagishi-cho, Chuo-ku, Niigata 951-8566, Japan

Int. J. Mol. Sci. 2016, 17(11), 1834; https://doi.org/10.3390/ijms17111834 - 3 Nov 2016

Cited by 5 | Viewed by 5133

Abstract

Our objectives were to (1) investigate the feasibility of the use of the Japanese version of the Hopkins Verbal Learning Test-Revised (HVLT-R); (2) identify the clinical factors influencing the HVLT-R scores of patients undergoing whole-brain radiation therapy (WBRT); and (3) compare the neurocognitive [...] Read more.

Our objectives were to (1) investigate the feasibility of the use of the Japanese version of the Hopkins Verbal Learning Test-Revised (HVLT-R); (2) identify the clinical factors influencing the HVLT-R scores of patients undergoing whole-brain radiation therapy (WBRT); and (3) compare the neurocognitive function (NCF) after WBRT in different dose fractionation schedules. We administered the HVLT-R (Japanese version) before (baseline) and at four and eight months after WBRT in 45 patients who received either therapeutic (35Gy-in-14, n = 16; 30Gy-in-10, n = 18) or prophylactic (25Gy-in-10, n = 11) WBRT. Sixteen patients dropped out before the eight-month examination, due mostly to death from cancer. The Karnofsky Performance Status (KPS) 80–100 group had significantly higher baseline total recall (TR) scores (p = 0.0053), delayed recall (DR) scores (p = 0.012), and delayed recognition (DRecog) scores (p = 0.0078). The patients aged ≤65 years also had significantly higher TR scores (p = 0.030) and DRecog scores (p = 0.031). The patients who underwent two examinations (worse-prognosis group) had significantly decreased DR scores four months after WBRT compared to the baseline (p = 0.0073), and they were significantly more likely to have declined individual TR scores (p = 0.0017) and DR scores (p = 0.035) at four months. The eight-month HVLT-R scores did not significantly decline regardless of the WBRT dose fractionation. The baseline NCF was determined by age and KPS, and the early decline in NCF is characteristic of the worse-prognosis group. Full article

(This article belongs to the Special Issue Brain Metastasis 2016)

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15 pages, 4071 KiB

Open AccessArticle

Salvianolic Acid B (Sal B) Protects Retinal Pigment Epithelial Cells from Oxidative Stress-Induced Cell Death by Activating Glutaredoxin 1 (Grx1)

by Xiaobin Liu¹, Christy Xavier¹, Jamieson Jann¹ and Hongli Wu^1,2,*

¹ Pharmaceutical Sciences, University of North Texas System College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX 76107, USA

² North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX 76107, USA

Int. J. Mol. Sci. 2016, 17(11), 1835; https://doi.org/10.3390/ijms17111835 - 3 Nov 2016

Cited by 33 | Viewed by 7761

Abstract

Protein glutathionylation, defined as the formation of protein mixed disulfides (PSSG) between cysteine residues and glutathione (GSH), can lead to cell death. Glutaredoxin 1 (Grx1) is a thiol repair enzyme which catalyzes the reduction of PSSG. Therefore, Grx1 exerts strong anti-apoptotic effects by [...] Read more.

Protein glutathionylation, defined as the formation of protein mixed disulfides (PSSG) between cysteine residues and glutathione (GSH), can lead to cell death. Glutaredoxin 1 (Grx1) is a thiol repair enzyme which catalyzes the reduction of PSSG. Therefore, Grx1 exerts strong anti-apoptotic effects by improving the redox state, especially in times of oxidative stress. However, there is currently no compound that is identified as a Grx1 activator. In this study, we identified and characterized Salvianolic acid B (Sal B), a natural compound, as a Grx1 inducer, which potently protected retinal pigment epithelial (RPE) cells from oxidative injury. Our results showed that treatment with Sal B protected primary human RPE cells from H₂O₂-induced cell damage. Interestingly, we found Sal B pretreatment upregulated Grx1 expression in RPE cells in a time- and dose-dependent manner. Furthermore, NF-E2-related factor 2 (Nrf2), the key transcription factor that regulates the expression of Grx1, was activated in Sal B treated RPE cells. Further investigation showed that knockdown of Grx1 by small interfering RNA (siRNA) significantly reduced the protective effects of Sal B. We conclude that Sal B protects RPE cells against H₂O₂-induced cell injury through Grx1 induction by activating Nrf2 pathway, thus preventing lethal accumulation of PSSG and reversing oxidative damage. Full article

(This article belongs to the Special Issue Neuroprotective Strategies 2016)

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9 pages, 650 KiB

Open AccessArticle

The Chemical Composition of Essential Oils from Cinnamomum camphora and Their Insecticidal Activity against the Stored Product Pests

by Shanshan Guo¹, Zhufeng Geng^1,2, Wenjuan Zhang¹

, Junyu Liang¹, Chengfang Wang^1,*, Zhiwei Deng^1,2 and Shushan Du^1,*

¹ Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China

² Analytical and Testing Center, Beijing Normal University, Beijing 100875, China

Int. J. Mol. Sci. 2016, 17(11), 1836; https://doi.org/10.3390/ijms17111836 - 4 Nov 2016

Cited by 122 | Viewed by 9382

Abstract

To investigate the chemical composition and insecticidal activity of the essential oils of certain Chinese medicinal herbs and spices, the essential oils were extracted from the stem barks, leaves, and fruits of Cinnamomum camphora (L.) Presl, which were found to possess strong fumigant [...] Read more.

To investigate the chemical composition and insecticidal activity of the essential oils of certain Chinese medicinal herbs and spices, the essential oils were extracted from the stem barks, leaves, and fruits of Cinnamomum camphora (L.) Presl, which were found to possess strong fumigant toxicity against Tribolium castaneum and Lasioderma serricorne adults. The essential oils of the plants were extracted by the method of steam distillation using a Clavenger apparatus. Their composition was determined by gas chromatography/mass spectrometric (GC-MS) analyses (HP-5MS column), and their insecticidal activity was measured by seal-spaced fumigation. D-camphor (51.3%), 1,8-cineole (4.3%), and α-terpineol (3.8%), while D-camphor (28.1%), linalool (22.9%), and 1,8-cineole (5.3%) were the main constituents of its fruits. The essential oils of the C. camphora all showed fumigant and contact toxicity. Other compounds exhibited various levels of bioactivities. The results indicate that the essential oils of C. camphora and its individual compounds can be considered a natural resource for the two stored-product insect management. Full article

(This article belongs to the Special Issue Bioactive Phytochemicals and Functional Food Ingredients in Fruits and Vegetables 2016)

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19 pages, 7364 KiB

Open AccessArticle

Comparative Transcriptional Analysis of Loquat Fruit Identifies Major Signal Networks Involved in Fruit Development and Ripening Process

by Huwei Song^1,2,*

, Xiangxiang Zhao^1,2, Weicheng Hu^1,2

, Xinfeng Wang^1,2, Ting Shen^1,2 and Liming Yang^1,2

¹ Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, College of Life Science, Huaiyin Normal University, Huai’an 223300, Jiangsu, China

² Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huai’an 223300, Jiangsu, China

Int. J. Mol. Sci. 2016, 17(11), 1837; https://doi.org/10.3390/ijms17111837 - 4 Nov 2016

Cited by 16 | Viewed by 6394

Abstract

Loquat (Eriobotrya japonica Lindl.) is an important non-climacteric fruit and rich in essential nutrients such as minerals and carotenoids. During fruit development and ripening, thousands of the differentially expressed genes (DEGs) from various metabolic pathways cause a series of physiological and biochemical [...] Read more.

Loquat (Eriobotrya japonica Lindl.) is an important non-climacteric fruit and rich in essential nutrients such as minerals and carotenoids. During fruit development and ripening, thousands of the differentially expressed genes (DEGs) from various metabolic pathways cause a series of physiological and biochemical changes. To better understand the underlying mechanism of fruit development, the Solexa/Illumina RNA-seq high-throughput sequencing was used to evaluate the global changes of gene transcription levels. More than 51,610,234 high quality reads from ten runs of fruit development were sequenced and assembled into 48,838 unigenes. Among 3256 DEGs, 2304 unigenes could be annotated to the Gene Ontology database. These DEGs were distributed into 119 pathways described in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A large number of DEGs were involved in carbohydrate metabolism, hormone signaling, and cell-wall degradation. The real-time reverse transcription (qRT)-PCR analyses revealed that several genes related to cell expansion, auxin signaling and ethylene response were differentially expressed during fruit development. Other members of transcription factor families were also identified. There were 952 DEGs considered as novel genes with no annotation in any databases. These unigenes will serve as an invaluable genetic resource for loquat molecular breeding and postharvest storage. Full article

(This article belongs to the Special Issue Ripening Control and Induction of the Defence and Antioxidant Systems)

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14 pages, 2582 KiB

Open AccessArticle

Tapirira guianensis Aubl. Extracts Inhibit Proliferation and Migration of Oral Cancer Cells Lines

by Renato José Silva-Oliveira¹, Gabriela Francine Lopes², Luiz Fernando Camargos³, Ana Maciel Ribeiro⁴, Fábio Vieira dos Santos³, Richele Priscila Severino⁵, Vanessa Gisele Pasqualotto Severino⁵, Ana Paula Terezan⁵, Ralph Gruppi Thomé⁶, Hélio Batista dos Santos⁶, Rui Manuel Reis^1,7,8 and Rosy Iara Maciel de Azambuja Ribeiro^3,*

¹ Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil

² Laboratory of Experimental Pathology, Federal University of São João del Rei—CCO/UFSJ, Divinópolis 35501-296, Brazil

³ Laboratory of Mutagenesis, Federal University of São João del Rei—CCO/UFSJ, Divinópolis 35501-296, Brazil

⁴ Medical School, Federal University of Minas Gerais—UFMG, Belo Horizonte 31270-901, Brazil

⁵ Special Academic Unit of Physics and Chemistry, Federal University of Goiás, Catalão 75704-020, Brazil

⁶ Laboratory of Tissue Processing, Federal University of São João del Rei—CCO/UFSJ, Divinópolis 35501-296, Brazil

⁷ Life and Health Sciences Research Institute (ICVS), Health Sciences School, University of Minho, Braga 4710-057, Portugal

⁸ 3ICVS/3B’s-PT Government Associate Laboratory, Braga 4710-057, Portugal

Int. J. Mol. Sci. 2016, 17(11), 1839; https://doi.org/10.3390/ijms17111839 - 8 Nov 2016

Cited by 13 | Viewed by 5871

Abstract

Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer [...] Read more.

Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of T. guianensis was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of T. guianensis extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy. Full article

(This article belongs to the Special Issue Biological Activity of Natural Secondary Metabolite Products)

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14 pages, 1026 KiB

Open AccessReview

Transcriptional and Posttranslational Regulation of Nucleotide Excision Repair: The Guardian of the Genome against Ultraviolet Radiation

by Jeong-Min Park and Tae-Hong Kang^*

Department of Biological Science, Dong-A University, Busan 49315, Korea

Int. J. Mol. Sci. 2016, 17(11), 1840; https://doi.org/10.3390/ijms17111840 - 4 Nov 2016

Cited by 34 | Viewed by 8557

Abstract

Ultraviolet (UV) radiation from sunlight represents a constant threat to genome stability by generating modified DNA bases such as cyclobutane pyrimidine dimers (CPD) and pyrimidine-pyrimidone (6-4) photoproducts (6-4PP). If unrepaired, these lesions can have deleterious effects, including skin cancer. Mammalian cells are able [...] Read more.

Ultraviolet (UV) radiation from sunlight represents a constant threat to genome stability by generating modified DNA bases such as cyclobutane pyrimidine dimers (CPD) and pyrimidine-pyrimidone (6-4) photoproducts (6-4PP). If unrepaired, these lesions can have deleterious effects, including skin cancer. Mammalian cells are able to neutralize UV-induced photolesions through nucleotide excision repair (NER). The NER pathway has multiple components including seven xeroderma pigmentosum (XP) proteins (XPA to XPG) and numerous auxiliary factors, including ataxia telangiectasia and Rad3-related (ATR) protein kinase and RCC1 like domain (RLD) and homologous to the E6-AP carboxyl terminus (HECT) domain containing E3 ubiquitin protein ligase 2 (HERC2). In this review we highlight recent data on the transcriptional and posttranslational regulation of NER activity. Full article

(This article belongs to the Collection Radiation Toxicity in Cells)

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18 pages, 2761 KiB

Open AccessReview

Lipidomics—Reshaping the Analysis and Perception of Type 2 Diabetes

by Daniel F. Markgraf^1,2, Hadi Al-Hasani^2,3 and Stefan Lehr^2,3,*

¹ Institute for Clinical Diabetology, German Diabetes Center, c/o Auf’m Hennekamp 65, D-40225 Düsseldorf, Germany

² German Center for Diabetes Research (DZD e.V.), München, D-85764 Neuherberg, Germany

³ Institute for Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, c/o Auf’m Hennekamp 65, D-40225 Düsseldorf, Germany

Int. J. Mol. Sci. 2016, 17(11), 1841; https://doi.org/10.3390/ijms17111841 - 4 Nov 2016

Cited by 49 | Viewed by 10811

Abstract

As a consequence of a sedentary lifestyle as well as changed nutritional behavior, today’s societies are challenged by the rapid propagation of metabolic disorders. A common feature of diseases, such as obesity and type 2 diabetes (T2D), is the dysregulation of lipid metabolism. [...] Read more.

As a consequence of a sedentary lifestyle as well as changed nutritional behavior, today’s societies are challenged by the rapid propagation of metabolic disorders. A common feature of diseases, such as obesity and type 2 diabetes (T2D), is the dysregulation of lipid metabolism. Our understanding of the mechanisms underlying these diseases is hampered by the complexity of lipid metabolic pathways on a cellular level. Furthermore, overall lipid homeostasis in higher eukaryotic organisms needs to be maintained by a highly regulated interplay between tissues, such as adipose tissue, liver and muscle. Unraveling pathological mechanisms underlying metabolic disorders therefore requires a diversified approach, integrating basic cellular research with clinical research, ultimately relying on the analytical power of mass spectrometry-based techniques. Here, we discuss recent progress in the development of lipidomics approaches to resolve the pathological mechanisms of metabolic diseases and to identify suitable biomarkers for clinical application. Due to its growing impact worldwide, we focus on T2D to highlight the key role of lipidomics in our current understanding of this disease, discuss remaining questions and suggest future strategies to address them. Full article

(This article belongs to the Special Issue Lipidomics and Glycomics: New Advances in Food Science and Nutrition)

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19 pages, 6934 KiB

Open AccessArticle

p-Hydroxyphenyl-pyranoanthocyanins: An Experimental and Theoretical Investigation of Their Acid—Base Properties and Molecular Interactions

by Anna Vallverdú-Queralt^1,2, Michal Biler^3,4, Emmanuelle Meudec¹, Christine Le Guernevé¹, Aude Vernhet¹, Jean-Paul Mazauric¹, Jean-Luc Legras¹, Michèle Loonis⁵, Patrick Trouillas^3,6, Véronique Cheynier^1,* and Olivier Dangles⁵

¹ Institut National de la Recherche Agronomique (INRA), UMR1083 Sciences pour l’œnologie, 2 place Pierre Viala, 34060 Montpellier CEDEX, France

² CIBER Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, 28903 Madrid, Spain

³ INSERM UMR 850, University of Limoges, School of Pharmacy, 2 rue du Dr. Marcland, F-87025 Limoges, France

⁴ Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University, Šlechtitelů 27, 78371 Olomouc, Czech Republic

⁵ INRA, UMR408 SQPOV, University of Avignon, 84000 Avignon, France

⁶ Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University Olomouc, tř. 17. listopadu 12, 77146 Olomouc, Czech Republic

Int. J. Mol. Sci. 2016, 17(11), 1842; https://doi.org/10.3390/ijms17111842 - 5 Nov 2016

Cited by 31 | Viewed by 6440

Abstract

The physicochemical properties of the wine pigments catechyl-pyranomalvidin-3-O-glucoside (PA1) and guaiacyl-pyranomalvidin-3-O-glucoside (PA2) are extensively revisited using ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and quantum chemistry density functional theory (DFT) calculations. In mildly acidic aqueous solution, each cationic pigment [...] Read more.

The physicochemical properties of the wine pigments catechyl-pyranomalvidin-3-O-glucoside (PA1) and guaiacyl-pyranomalvidin-3-O-glucoside (PA2) are extensively revisited using ultraviolet (UV)-visible spectroscopy, dynamic light scattering (DLS) and quantum chemistry density functional theory (DFT) calculations. In mildly acidic aqueous solution, each cationic pigment undergoes regioselective deprotonation to form a single neutral quinonoid base and water addition appears negligible. Above pH = 4, both PA1 and PA2 become prone to aggregation, which is manifested by the slow build-up of broad absorption bands at longer wavelengths (λ ≥ 600 nm), followed in the case of PA2 by precipitation. Some phenolic copigments are able to inhibit aggregation of pyranoanthocyanins (PAs), although at large copigment/PA molar ratios. Thus, chlorogenic acid can dissociate PA1 aggregates while catechin is inactive. With PA2, both chlorogenic acid and catechin are able to prevent precipitation but not self-association. Calculations confirmed that the noncovalent dimerization of PAs is stronger with the neutral base than with the cation and also stronger than π–π stacking of PAs to chlorogenic acid (copigmentation). For each type of complex, the most stable conformation could be obtained. Finally, PA1 can also bind hard metal ions such as Al³⁺ and Fe³⁺ and the corresponding chelates are less prone to self-association. Full article

(This article belongs to the Special Issue Anthocyanins)

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13 pages, 2031 KiB

Open AccessArticle

The Complete Mitochondrial Genome of Aleurocanthus camelliae: Insights into Gene Arrangement and Genome Organization within the Family Aleyrodidae

by Shi-Chun Chen¹

, Xiao-Qing Wang¹, Pin-Wu Li², Xiang Hu¹, Jin-Jun Wang³

and Ping Peng^1,*

¹ Tea Research Institute, Chongqing Academy of Agricultural Science, Chongqing 402160, China

² Tea Science Department, College of Horticulture, Sichuan Agricultural University, Ya’an 611130, China

³ Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China

Int. J. Mol. Sci. 2016, 17(11), 1843; https://doi.org/10.3390/ijms17111843 - 7 Nov 2016

Cited by 14 | Viewed by 4980

Abstract

There are numerous gene rearrangements and transfer RNA gene absences existing in mitochondrial (mt) genomes of Aleyrodidae species. To understand how mt genomes evolved in the family Aleyrodidae, we have sequenced the complete mt genome of Aleurocanthus camelliae and comparatively analyzed all reported [...] Read more.

There are numerous gene rearrangements and transfer RNA gene absences existing in mitochondrial (mt) genomes of Aleyrodidae species. To understand how mt genomes evolved in the family Aleyrodidae, we have sequenced the complete mt genome of Aleurocanthus camelliae and comparatively analyzed all reported whitefly mt genomes. The mt genome of A. camelliae is 15,188 bp long, and consists of 13 protein-coding genes, two rRNA genes, 21 tRNA genes and a putative control region (GenBank: KU761949). The tRNA gene, trnI, has not been observed in this genome. The mt genome has a unique gene order and shares most gene boundaries with Tetraleurodes acaciae. Nineteen of 21 tRNA genes have the conventional cloverleaf shaped secondary structure and two (trnS₁ and trnS₂) lack the dihydrouridine (DHU) arm. Using ARWEN and homologous sequence alignment, we have identified five tRNA genes and revised the annotation for three whitefly mt genomes. This result suggests that most absent genes exist in the genomes and have not been identified, due to be lack of technology and inference sequence. The phylogenetic relationships among 11 whiteflies and Drosophila melanogaster were inferred by maximum likelihood and Bayesian inference methods. Aleurocanthus camelliae and T. acaciae form a sister group, and all three Bemisia tabaci and two Bemisia afer strains gather together. These results are identical to the relationships inferred from gene order. We inferred that gene rearrangement plays an important role in the mt genome evolved from whiteflies. Full article

(This article belongs to the Section Biochemistry)

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14 pages, 1424 KiB

Open AccessArticle

Anti-Melanogenic Activities of Heracleum moellendorffii via ERK1/2-Mediated MITF Downregulation

by Md Badrul Alam^†

, Bum-Ju Seo^†, Peijun Zhao and Sang-Han Lee^*

¹ Department of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Korea

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1844; https://doi.org/10.3390/ijms17111844 - 4 Nov 2016

Cited by 28 | Viewed by 7332

Abstract

In this study, the anti-melanogenic effects of Heracleum moellendorffii Hance extract (HmHe) and the mechanisms through which it inhibits melanogenesis in melan-a cells were investigated. Mushroom tyrosinase (TYR) activity and melanin content as well as cellular tyrosinase activity were measured in the cells. [...] Read more.

In this study, the anti-melanogenic effects of Heracleum moellendorffii Hance extract (HmHe) and the mechanisms through which it inhibits melanogenesis in melan-a cells were investigated. Mushroom tyrosinase (TYR) activity and melanin content as well as cellular tyrosinase activity were measured in the cells. mRNA and protein expression of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), TYR-related protein-1 (TYRP-1) and -2 were also examined. The results demonstrate that treatment with HmHe significantly inhibits mushroom tyrosinase activity. Furthermore, HmHe also markedly inhibits melanin production and intracellular tyrosinase activity. By suppressing the expression of TYR, TYRP-1, TYRP-2, and MITF, HmHe treatment antagonized melanin production in melan-a cells. Additionally, HmHe interfered with the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, with reversal of HmHe-induced melanogenesis inhibition after treatment with specific inhibitor U0126. In summary, HmHe can be said to stimulate ERK1/2 phosphorylation and subsequent degradation of MITF, resulting in suppression of melanogenic enzymes and melanin production, possibly due to the presence of polyphenolic compounds. Full article

(This article belongs to the Special Issue Bioactive Phytochemicals and Functional Food Ingredients in Fruits and Vegetables 2016)

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15 pages, 2115 KiB

Open AccessArticle

Cell-Free RNA Content in Peripheral Blood as Potential Biomarkers for Detecting Circulating Tumor Cells in Non-Small Cell Lung Carcinoma

by Xin-Min Yu^1,2,3,†, Yi-Chen Wu^1,†, Xiang Liu¹, Xian-Cong Huang¹, Xiu-Xiu Hou¹, Jiu-Li Wang¹, Xiang-Liu Cheng¹, Wei-Min Mao^1,3 and Zhi-Qiang Ling^1,3,*

¹ Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, Hangzhou 310022, China

² Department of Thoracic Tumor Chemotherapy, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, Hangzhou 310022, China

³ Zhejiang Key Laboratory of Diagnosis & Treatment Technology on Thoracic Oncology (Lung and Esophagus), Hangzhou 310022, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1845; https://doi.org/10.3390/ijms17111845 - 5 Nov 2016

Cited by 14 | Viewed by 5817

Abstract

Circulating tumor cells (CTCs) have been implicated in tumor progression and prognosis. Techniques detecting CTCs in the peripheral blood of patients with non-small cell lung carcinoma (NSCLC) may help to identify individuals likely to benefit from early systemic treatment. However, the detection of [...] Read more.

Circulating tumor cells (CTCs) have been implicated in tumor progression and prognosis. Techniques detecting CTCs in the peripheral blood of patients with non-small cell lung carcinoma (NSCLC) may help to identify individuals likely to benefit from early systemic treatment. However, the detection of CTCs with a single marker is challenging, owing to low specificity and sensitivity and due to the heterogeneity and rareness of CTCs. Herein, the probability of cell-free RNA content in the peripheral blood as a potential biomarker for detecting CTCs in cancer patients was investigated. An immunomagnetic enrichment of real-time reverse-transcription PCR (RT-PCR) technology for analysis of CTCs in NSCLC patients was also developed. The mRNA levels of four candidate genes, cytokeratin 7 (CK7), E74-like factor 3 (ELF3), epidermal growth factor receptor (EGFR), and erythropoietin-producing hepatocellular carcinoma receptor B4 (EphB4) that were significantly elevated in tumor tissues and peripheral blood mononuclear cells (PBMCs) were determined. The expression of CK7 and ELF3 in tumor tissues and EGFR in PBMCs was associated with lymph node metastasis (all p < 0.05). The expression of CK7 in PBMCs was correlated with age and EphB4 in PBMCs correlated with histopathological type, respectively (all p < 0.05). The expression of all four genes in tumor tissues and PBMCs was significantly correlated with the clinical stage (all p < 0.01). Survival analysis showed that the patients with enhanced expression of CK7, ELF3, EGFR, and EphB4 mRNA in PBMCs had poorer disease-free survival (DFS) and overall survival (OS) than those without (all p < 0.0001). The present study showed that this alteration of cell-free RNA content in peripheral blood might have clinical ramifications in the diagnosis and treatment of NSCLC patients. Full article

(This article belongs to the Special Issue Liquid Biopsy for Clinical Application)

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14 pages, 8300 KiB

Open AccessArticle

A Novel Role of Dickkopf-Related Protein 3 in Macropinocytosis in Human Bladder Cancer T24 Cells

by Nonoka Tsujimura^1,†, Nami O. Yamada^1,2,*,†, Yuki Kuranaga^1,†

, Minami Kumazaki¹, Haruka Shinohara¹, Kohei Taniguchi¹

and Yukihiro Akao¹

¹ United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 1-1 Yanagido, Gifu-city, Gifu 501-1194, Japan

² Department of Anatomy, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu-city, Gifu 501-1194, Japan

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1846; https://doi.org/10.3390/ijms17111846 - 5 Nov 2016

Cited by 5 | Viewed by 5313

Abstract

Dickkopf-related protein 3 (Dkk-3) is a potential tumor suppressor reported in various cancer entities. However, we found that Dkk-3 was exceptionally upregulated in bladder cancer T24 cells. To validate the biological role of Dkk-3 other than a tumor suppressor, we examined the function [...] Read more.

Dickkopf-related protein 3 (Dkk-3) is a potential tumor suppressor reported in various cancer entities. However, we found that Dkk-3 was exceptionally upregulated in bladder cancer T24 cells. To validate the biological role of Dkk-3 other than a tumor suppressor, we examined the function of Dkk-3 in T24 cells. Gene silencing of Dkk-3 inhibited cell growth through inducing G₀/G₁ cell-cycle arrest. Furthermore, Dkk-3 knock-down caused macropinocytosis accompanied by autophagy, which were canceled in part by their inhibitors 5-(N-ethyl-N-isopropyl) amiloride (EIPA) and 3-methyladenine (3-MA). The macropinocytosis was induced by the Dkk-3 knock-down when there were sufficient extracellular nutrients. On the other hand, when the nutritional condition was poor, the autophagy was mainly induced by the Dkk-3 knock-down. These data indicated that Dkk-3 has a role in modulating macropinocytotic and autophagic pathways, a distinct function other than a Wnt antagonist. Full article

(This article belongs to the Special Issue Molecular Classification of Human Cancer: Diagnosis and Treatment 2016)

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15 pages, 5964 KiB

Open AccessArticle

Hydrostatic Compress Force Enhances the Viability and Decreases the Apoptosis of Condylar Chondrocytes through Integrin-FAK-ERK/PI3K Pathway

by Dandan Ma^1,2, Xiaoxing Kou³, Jing Jin¹, Taotao Xu⁴, Mengjie Wu⁵, Liquan Deng¹, Lusi Fu¹, Yi Liu², Gang Wu^2,*

and Haiping Lu^1,*

¹ School of Stomatology, Zhejiang Chinese Medical University, Hangzhou 310053, China

² Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), VU University Amsterdam and University of Amsterdam, MOVE Research Institute, Gustav Mahlerlaan 3004, Amsterdam 1081LA, Nord-Holland, The Netherlands

³ Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China

⁴ Department of Orthopaedic Surgery, the First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China

⁵ Department of Orthodontics, Stomatology Hospital Affiliated to Zhejiang University, Hangzhou 310053, China

Int. J. Mol. Sci. 2016, 17(11), 1847; https://doi.org/10.3390/ijms17111847 - 7 Nov 2016

Cited by 19 | Viewed by 6715

Abstract

Reduced mechanical stimuli in many pathological cases, such as hemimastication and limited masticatory movements, can significantly affect the metabolic activity of mandibular condylar chondrocytes and the growth of mandibles. However, the molecular mechanisms for these phenomena remain unclear. In this study, we hypothesized [...] Read more.

Reduced mechanical stimuli in many pathological cases, such as hemimastication and limited masticatory movements, can significantly affect the metabolic activity of mandibular condylar chondrocytes and the growth of mandibles. However, the molecular mechanisms for these phenomena remain unclear. In this study, we hypothesized that integrin-focal adhesion kinase (FAK)-ERK (extracellular signal–regulated kinase)/PI3K (phosphatidylinositol-3-kinase) signaling pathway mediated the cellular response of condylar chondrocytes to mechanical loading. Primary condylar chondrocytes were exposed to hydrostatic compressive forces (HCFs) of different magnitudes (0, 50, 100, 150, 200, and 250 kPa) for 2 h. We measured the viability, morphology, and apoptosis of the chondrocytes with different treatments as well as the gene, protein expression, and phosphorylation of mechanosensitivity-related molecules, such as integrin α2, integrin α5, integrin β1, FAK, ERK, and PI3K. HCFs could significantly increase the viability and surface area of condylar chondrocytes and decrease their apoptosis in a dose-dependent manner. HCF of 250 kPa resulted in a 1.51 ± 0.02-fold increase of cell viability and reduced the ratio of apoptotic cells from 18.10% ± 0.56% to 7.30% ± 1.43%. HCFs could significantly enhance the mRNA and protein expression of integrin α2, integrin α5, and integrin β1 in a dose-dependent manner, but not ERK1, ERK2, or PI3K. Instead, HCF could significantly increase phosphorylation levels of FAK, ERK1/2, and PI3K in a dose-dependent manner. Cilengitide, the potent integrin inhibitor, could dose-dependently block such effects of HCFs. HCFs enhances the viability and decreases the apoptosis of condylar chondrocytes through the integrin-FAK-ERK/PI3K pathway. Full article

(This article belongs to the Special Issue Apoptotic Chondrocytes and Osteoarthritis)

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12 pages, 7581 KiB

Open AccessArticle

IBTK Differently Modulates Gene Expression and RNA Splicing in HeLa and K562 Cells

by Giuseppe Fiume^1,*

, Annarita Scialdone^1,†, Francesca Rizzo²

, Maria Rosaria De Filippo^2,‡, Carmelo Laudanna², Francesco Albano¹

, Gaetanina Golino¹, Eleonora Vecchio¹

, Marilena Pontoriero¹, Selena Mimmi¹, Simona Ceglia¹, Antonio Pisano¹, Enrico Iaccino¹

, Camillo Palmieri¹

, Sergio Paduano³, Giuseppe Viglietto¹, Alessandro Weisz²

, Giuseppe Scala^1,* and Ileana Quinto^1,*

¹ Department of Experimental and Clinical Medicine, University ‘Magna Graecia’ of Catanzaro, Viale Europa (Località Germaneto), 88100 Catanzaro, Italy

² Laboratory of Molecular Medicine and Genomics, Department of Medicine, Surgery and Dentistry ‘Schola Medica Salernitana’, University of Salerno, via S. Allende 1, 84081 Baronissi, Italy

³ Department of Health Sciences, University “Magna Graecia”, 88100 Catanzaro, Italy

^† Present address: Department of Hematology and Transfusion Medicine, Medical Faculty, University of Lund, 221 00 Lund, Sweden.

^‡ Present address: Center for Translational Genomics and Bioinformatics, Ospedale San Raffaele, 20132 Milano, Italy.

Int. J. Mol. Sci. 2016, 17(11), 1848; https://doi.org/10.3390/ijms17111848 - 7 Nov 2016

Cited by 14 | Viewed by 6605

Abstract

The IBTK gene encodes the major protein isoform IBTKα that was recently characterized as substrate receptor of Cul3-dependent E3 ligase, regulating ubiquitination coupled to proteasomal degradation of Pdcd4, an inhibitor of translation. Due to the presence of Ankyrin-BTB-RCC1 domains that mediate several protein-protein [...] Read more.

The IBTK gene encodes the major protein isoform IBTKα that was recently characterized as substrate receptor of Cul3-dependent E3 ligase, regulating ubiquitination coupled to proteasomal degradation of Pdcd4, an inhibitor of translation. Due to the presence of Ankyrin-BTB-RCC1 domains that mediate several protein-protein interactions, IBTKα could exert expanded regulatory roles, including interaction with transcription regulators. To verify the effects of IBTKα on gene expression, we analyzed HeLa and K562 cell transcriptomes by RNA-Sequencing before and after IBTK knock-down by shRNA transduction. In HeLa cells, 1285 (2.03%) of 63,128 mapped transcripts were differentially expressed in IBTK-shRNA-transduced cells, as compared to cells treated with control-shRNA, with 587 upregulated (45.7%) and 698 downregulated (54.3%) RNAs. In K562 cells, 1959 (3.1%) of 63128 mapped RNAs were differentially expressed in IBTK-shRNA-transduced cells, including 1053 upregulated (53.7%) and 906 downregulated (46.3%). Only 137 transcripts (0.22%) were commonly deregulated by IBTK silencing in both HeLa and K562 cells, indicating that most IBTKα effects on gene expression are cell type-specific. Based on gene ontology classification, the genes responsive to IBTK are involved in different biological processes, including in particular chromatin and nucleosomal organization, gene expression regulation, and cellular traffic and migration. In addition, IBTK RNA interference affected RNA maturation in both cell lines, as shown by the evidence of alternative 3′- and 5′-splicing, mutually exclusive exons, retained introns, and skipped exons. Altogether, these results indicate that IBTK differently modulates gene expression and RNA splicing in HeLa and K562 cells, demonstrating a novel biological role of this protein. Full article

(This article belongs to the Special Issue Next-Generation Sequencing for Clinical Application)

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24 pages, 1484 KiB

Open AccessReview

The Bromodomain and Extra-Terminal Domain (BET) Family: Functional Anatomy of BET Paralogous Proteins

by Yasushi Taniguchi

Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Isehara, Kanagawa 259-1193, Japan

Int. J. Mol. Sci. 2016, 17(11), 1849; https://doi.org/10.3390/ijms17111849 - 7 Nov 2016

Cited by 256 | Viewed by 19054

Abstract

The Bromodomain and Extra-Terminal Domain (BET) family of proteins is characterized by the presence of two tandem bromodomains and an extra-terminal domain. The mammalian BET family of proteins comprises BRD2, BRD3, BRD4, and BRDT, which are encoded by paralogous genes that may have [...] Read more.

The Bromodomain and Extra-Terminal Domain (BET) family of proteins is characterized by the presence of two tandem bromodomains and an extra-terminal domain. The mammalian BET family of proteins comprises BRD2, BRD3, BRD4, and BRDT, which are encoded by paralogous genes that may have been generated by repeated duplication of an ancestral gene during evolution. Bromodomains that can specifically bind acetylated lysine residues in histones serve as chromatin-targeting modules that decipher the histone acetylation code. BET proteins play a crucial role in regulating gene transcription through epigenetic interactions between bromodomains and acetylated histones during cellular proliferation and differentiation processes. On the other hand, BET proteins have been reported to mediate latent viral infection in host cells and be involved in oncogenesis. Human BRD4 is involved in multiple processes of the DNA virus life cycle, including viral replication, genome maintenance, and gene transcription through interaction with viral proteins. Aberrant BRD4 expression contributes to carcinogenesis by mediating hyperacetylation of the chromatin containing the cell proliferation-promoting genes. BET bromodomain blockade using small-molecule inhibitors gives rise to selective repression of the transcriptional network driven by c-MYC These inhibitors are expected to be potential therapeutic drugs for a wide range of cancers. This review presents an overview of the basic roles of BET proteins and highlights the pathological functions of BET and the recent developments in cancer therapy targeting BET proteins in animal models. Full article

(This article belongs to the Special Issue Advances in Molecular Oncology)

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8 pages, 2257 KiB

Open AccessArticle

NOD Scid Gamma Mice Are Permissive to Allogeneic HSC Transplantation without Prior Conditioning

by Tom Verbiest^1,2, Rosemary Finnon¹, Natalie Brown¹, Paul Finnon¹, Simon Bouffler¹ and Christophe Badie^1,*

¹ Cancer Mechanisms and Biomarkers Group, Radiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Didcot OX11 ORQ, UK

² CRUK & MRC Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford OX3 7DQ, UK

Int. J. Mol. Sci. 2016, 17(11), 1850; https://doi.org/10.3390/ijms17111850 - 7 Nov 2016

Cited by 8 | Viewed by 6917

Abstract

Scid hematopoietic stem cells (HSCs) have an intrinsic defect in their maintenance within the bone marrow (BM) niche which facilitates HSC transplantation without the absolute requirement of prior conditioning. Nevertheless, NOD scid mice have a significantly altered life span due to early development [...] Read more.

Scid hematopoietic stem cells (HSCs) have an intrinsic defect in their maintenance within the bone marrow (BM) niche which facilitates HSC transplantation without the absolute requirement of prior conditioning. Nevertheless, NOD scid mice have a significantly altered life span due to early development of thymic lymphomas, which compromises the ability to study the long-term fate of exogenous HSCs and their progeny. Here, we present data on the transplantation of HSCs into NOD scid gamma (NSG) mice to achieve long-term engraftment without prior conditioning. We transplanted allogeneic HSCs constitutively expressing the mCherry fluorescent marker into age-matched NSG mice and assessed donor chimerism 6 months post-transplantation. All transplanted NSG mice showed long-term myeloid and lymphoid cell chimerism. Also, in vivo irradiated HSCs showed long-term engraftment, although overall white blood cell (WBC) donor chimerism was lower compared with non-irradiated HSCs. Using this novel NSG transplantation model, we will be able to study the effects of low dose in vivo X-ray exposure on the long-term fate of HSCs, without the requirement of prior radio-ablation of the recipient, and thus leaving the recipient’s BM microenvironment uncompromised. In conclusion, we demonstrated for the first time that allogeneic HSCs from a different inbred strain can compete for niches in the BM compartment of NSG mice. Full article

(This article belongs to the Collection Radiation Toxicity in Cells)

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12 pages, 2122 KiB

Open AccessArticle

Fto-Deficiency Affects the Gene and MicroRNA Expression Involved in Brown Adipogenesis and Browning of White Adipose Tissue in Mice

by Justiina Ronkainen^1,2,3,*, Eleonora Mondini⁴, Francesca Cinti⁴, Saverio Cinti⁴, Sylvain Sebért^1,5, Markku J. Savolainen^1,2,3

and Tuire Salonurmi^1,2,3

¹ Biocenter Oulu, University of Oulu, FI-90220 Oulu, Finland

² Faculty of Medicine, Department of Internal Medicine, University of Oulu, FI-90220 Oulu, Finland

³ Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FI-90220 Oulu, Finland

⁴ Department of Experimental and Clinical Medicine, Marche Polytechnic University, IT-60126 Ancona, Italy

⁵ Center for Life-Course Health Research, University of Oulu, FI-90220 Oulu, Finland

Int. J. Mol. Sci. 2016, 17(11), 1851; https://doi.org/10.3390/ijms17111851 - 7 Nov 2016

Cited by 33 | Viewed by 8048

Abstract

Genetic variants in the fat mass- and obesity-associated gene Fto are linked to the onset of obesity in humans. The causal role of the FTO protein in obesity is supported by evidence obtained from transgenic mice; however, the underlying molecular pathways pertaining to [...] Read more.

Genetic variants in the fat mass- and obesity-associated gene Fto are linked to the onset of obesity in humans. The causal role of the FTO protein in obesity is supported by evidence obtained from transgenic mice; however, the underlying molecular pathways pertaining to the role of FTO in obesity have yet to be established. In this study, we investigate the Fto gene in mouse brown adipose tissue and in the browning process of white adipose tissue. We analyze distinct structural and molecular factors in brown and white fat depots of Fto-deficient mice under normal and obesogenic conditions. We report significant alterations in the morphology of adipose tissue depots and the expression of mRNA and microRNA related to brown adipogenesis and metabolism in Fto-deficient mice. Furthermore, we show that high-fat feeding does not attenuate the browning process of Fto-deficient white adipose tissue as observed in wild-type tissue, suggesting a triggering effect of the FTO pathways by the dietary environment. Full article

(This article belongs to the Special Issue Nutrigenomics of Risk Factors for Disease)

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13 pages, 2912 KiB

Open AccessArticle

X-Linked miRNAs Associated with Gender Differences in Rheumatoid Arthritis

by Olfa Khalifa^1,2, Yves-Marie Pers^1,2,3

, Rosanna Ferreira³, Audrey Sénéchal⁴, Christian Jorgensen^1,2,3, Florence Apparailly^1,2,3 and Isabelle Duroux-Richard^1,2,*

¹ Inserm, U1183, Institute for Regenerative Medicine and Biotherapies, CHU Saint Eloi, 80 Avenue Augustin Fliche, 34295 Montpellier, France

² University of Montpellier, Boulevard Henri IV, 34090 Montpellier, France

³ Clinical Department for Osteoarticular Diseases and Biotherapy, University Hospital Lapeyronie, 34295 Montpellier, France

⁴ Inserm, U1051, Institute for Neurosciences Montpellier, CHU Saint Eloi, 80 Avenue Augustin Fliche, 34295 Montpellier, France

Int. J. Mol. Sci. 2016, 17(11), 1852; https://doi.org/10.3390/ijms17111852 - 8 Nov 2016

Cited by 67 | Viewed by 7639

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease that predominantly affects women. MicroRNAs have emerged as crucial regulators of the immune system, whose expression is deregulated in RA. We aimed at quantifying the expression level of 14 miRNAs located on the X chromosome and [...] Read more.

Rheumatoid arthritis (RA) is an autoimmune disease that predominantly affects women. MicroRNAs have emerged as crucial regulators of the immune system, whose expression is deregulated in RA. We aimed at quantifying the expression level of 14 miRNAs located on the X chromosome and at identifying whether differences are associated with disease and/or sex. A case–control study of 21 RA patients and 22 age- and sex-matched healthy controls was performed on peripheral blood mononuclear cells. The expression level of five miRNAs (miR-221, miR-222, miR-532, miR-106a, and miR-98) was significantly different between RA and controls when stratifying by sex, and the expression level of four miRNAs (miR-222, miR-532, miR-98, and miR-92a) was significantly different between RA females and males. The expression quantitative trait loci (eQTL) analysis revealed a significant gender effect of the FoxP3 promoter polymorphism rs3761548A/C on miR-221, miR-222 and miR-532 expression levels, and of the FoxP3 polymorphism rs2232365A/G on miR-221 expression levels in PBMC of RA patients. These data further support the involvement of the X chromosome in RA susceptibility. X-linked miRNAs, in the context of sex differences, might provide novel insight into new molecular mechanisms and potential therapeutic targets in RA for disease treatment and prevention. Full article

(This article belongs to the Collection Regulation by Non-coding RNAs)

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14 pages, 1143 KiB

Open AccessReview

Diabetic Microvascular Disease and Pulmonary Fibrosis: The Contribution of Platelets and Systemic Inflammation

by Rekha Jagadapillai¹, Madhavi J. Rane^2,3, Xingyu Lin^1,4, Andrew M. Roberts^1,5, Gary W. Hoyle⁶, Lu Cai^1,7,8 and Evelyne Gozal^1,5,7,*

¹ Department of Pediatrics, School of Medicine, University of Louisville, Louisville, KY 40292, USA

² Medicine/Nephrology, School of Medicine, University of Louisville, Louisville, KY 40292, USA

³ Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY 40292, USA

⁴ Department of Thoracic Surgery, the First Hospital of Jilin University, Changchun 130021, China

⁵ Physiology, School of Medicine, University of Louisville, Louisville, KY 40292, USA

⁶ Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY 40292, USA

⁷ Pharmacology & Toxicology, School of Medicine, University of Louisville, Louisville, KY 40292, USA

⁸ Radiation Oncology, School of Medicine, University of Louisville, Louisville, KY 40292, USA

Int. J. Mol. Sci. 2016, 17(11), 1853; https://doi.org/10.3390/ijms17111853 - 8 Nov 2016

Cited by 38 | Viewed by 9371

Abstract

Diabetes is strongly associated with systemic inflammation and oxidative stress, but its effect on pulmonary vascular disease and lung function has often been disregarded. Several studies identified restrictive lung disease and fibrotic changes in diabetic patients and in animal models of diabetes. While [...] Read more.

Diabetes is strongly associated with systemic inflammation and oxidative stress, but its effect on pulmonary vascular disease and lung function has often been disregarded. Several studies identified restrictive lung disease and fibrotic changes in diabetic patients and in animal models of diabetes. While microvascular dysfunction is a well-known complication of diabetes, the mechanisms leading to diabetes-induced lung injury have largely been disregarded. We described the potential involvement of diabetes-induced platelet-endothelial interactions in perpetuating vascular inflammation and oxidative injury leading to fibrotic changes in the lung. Changes in nitric oxide synthase (NOS) activation and decreased NO bioavailability in the diabetic lung increase platelet activation and vascular injury and may account for platelet hyperreactivity reported in diabetic patients. Additionally, the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway has been reported to mediate pancreatic islet damage, and is implicated in the onset of diabetes, inflammation and vascular injury. Many growth factors and diabetes-induced agonists act via the JAK/STAT pathway. Other studies reported the contribution of the JAK/STAT pathway to the regulation of the pulmonary fibrotic process but the role of this pathway in the development of diabetic lung fibrosis has not been considered. These observations may open new therapeutic perspectives for modulating multiple pathways to mitigate diabetes onset or its pulmonary consequences. Full article

(This article belongs to the Special Issue Diabetic Complications: Pathophysiology, Mechanisms, and Therapies)

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17 pages, 10917 KiB

Open AccessArticle

Use of Atomic Force Microscopy to Study the Multi-Modular Interaction of Bacterial Adhesins to Mucins

by A. Patrick Gunning^1,*, Devon Kavanaugh¹, Elizabeth Thursby¹, Sabrina Etzold^1,2, Donald A. MacKenzie¹ and Nathalie Juge^1,*

¹ The Gut Health and Food Safety Institute Strategic Programme, Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, UK

² Division of Neonatology and Division of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, School of Medicine, University of California San Diego, 9500 Gilman Drive, San Diego, CA 92093-0715, USA

Int. J. Mol. Sci. 2016, 17(11), 1854; https://doi.org/10.3390/ijms17111854 - 8 Nov 2016

Cited by 31 | Viewed by 7765

Abstract

The mucus layer covering the gastrointestinal (GI) epithelium is critical in selecting and maintaining homeostatic interactions with our gut bacteria. However, the molecular details of these interactions are not well understood. Here, we provide mechanistic insights into the adhesion properties of the canonical [...] Read more.

The mucus layer covering the gastrointestinal (GI) epithelium is critical in selecting and maintaining homeostatic interactions with our gut bacteria. However, the molecular details of these interactions are not well understood. Here, we provide mechanistic insights into the adhesion properties of the canonical mucus-binding protein (MUB), a large multi-repeat cell–surface adhesin found in Lactobacillus inhabiting the GI tract. We used atomic force microscopy to unravel the mechanism driving MUB-mediated adhesion to mucins. Using single-molecule force spectroscopy we showed that MUB displayed remarkable adhesive properties favouring a nanospring-like adhesion model between MUB and mucin mediated by unfolding of the multiple repeats constituting the adhesin. We obtained direct evidence for MUB self-interaction; MUB–MUB followed a similar binding pattern, confirming that MUB modular structure mediated such mechanism. This was in marked contrast with the mucin adhesion behaviour presented by Galectin-3 (Gal-3), a mammalian lectin characterised by a single carbohydrate binding domain (CRD). The binding mechanisms reported here perfectly match the particular structural organization of MUB, which maximizes interactions with the mucin glycan receptors through its long and linear multi-repeat structure, potentiating the retention of bacteria within the outer mucus layer. Full article

(This article belongs to the Special Issue Glycan–Receptor Interaction)

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11 pages, 1539 KiB

Open AccessArticle

Bidirectional Estrogen-Like Effects of Genistein on Murine Experimental Autoimmune Ovarian Disease

by Qiao Ding^1,†, Yuxiao Wang^1,†, Na Li¹, Kexue Zhu², Jielun Hu¹, Sunan Wang³, Fan Zhu⁴ and Shaoping Nie^1,*

¹ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

² Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, China

³ Canadian Food and Wine Institute, Niagara College, 135 Taylor Road, Niagara-on-the-Lake, ON L0S 1J0, Canada

⁴ School of Chemical Sciences, University of Auckland, Private Bag 92019, New Zealand

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1855; https://doi.org/10.3390/ijms17111855 - 8 Nov 2016

Cited by 5 | Viewed by 5168

Abstract

This study was to investigate the bidirectional estrogen-like effects of genistein on murine experimental autoimmune ovarian disease (AOD). Female BALB/c mice were induced by immunization with a peptide from murine zona pellucida. The changes of estrous cycle, ovarian histomorphology were measured, and the [...] Read more.

This study was to investigate the bidirectional estrogen-like effects of genistein on murine experimental autoimmune ovarian disease (AOD). Female BALB/c mice were induced by immunization with a peptide from murine zona pellucida. The changes of estrous cycle, ovarian histomorphology were measured, and the levels of serum sex hormone were analyzed using radioimmunoassay. Proliferative responses of the ovary were also determined by immunohistochemistry. Administration of 25 or 45 mg/kg body weight genistein enhanced ovary development with changes in serum sex hormone levels and proliferative responses. Meanwhile, the proportions of growing and mature follicles increased and the incidence of autoimmune oophoritis decreased, which exhibited normal ovarian morphology in administration of 25 or 45 mg/kg body weight genistein, while a lower dose (5 mg/kg body weight genistein) produced the opposite effect. These findings suggest that genistein exerts bidirectional estrogen-like effects on murine experimental AOD, while a high dose (45 mg/kg body weight) of genistein may suppress AOD. Full article

(This article belongs to the Special Issue Lipidomics and Glycomics: New Advances in Food Science and Nutrition)

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16 pages, 1401 KiB

Open AccessReview

The Role of CC-Chemokines in the Regulation of Angiogenesis

by Anisyah Ridiandries^1,2, Joanne T. M. Tan^1,2

and Christina A. Bursill^1,2,*

¹ Heart Research Institute, 7 Eliza Street, Newtown, Sydney, NSW 2042, Australia

² Sydney Medical School, University of Sydney, Sydney, NSW 2050, Australia

Int. J. Mol. Sci. 2016, 17(11), 1856; https://doi.org/10.3390/ijms17111856 - 8 Nov 2016

Cited by 92 | Viewed by 10265

Abstract

Angiogenesis, the formation of new blood vessels, is critical for survival and in the regenerative response to tissue injury or ischemia. However, in diseases such as cancer and atherosclerosis, inflammation can cause unregulated angiogenesis leading to excessive neovascularization, which exacerbates disease. Current anti-angiogenic [...] Read more.

Angiogenesis, the formation of new blood vessels, is critical for survival and in the regenerative response to tissue injury or ischemia. However, in diseases such as cancer and atherosclerosis, inflammation can cause unregulated angiogenesis leading to excessive neovascularization, which exacerbates disease. Current anti-angiogenic therapies cause complete inhibition of both inflammatory and ischemia driven angiogenesis causing a range of side effects in patients. Specific inhibition of inflammation-driven angiogenesis would therefore be immensely valuable. Increasing evidence suggests that the CC-chemokine class promotes inflammation-driven angiogenesis, whilst there is little evidence for a role in ischemia-mediated angiogenesis. The differential regulation of angiogenesis by CC-chemokines suggests it may provide an alternate strategy to treat angiogenesis associated pathological diseases. The focus of this review is to highlight the significant role of the CC-chemokine class in inflammation, versus ischemia driven angiogenesis, and to discuss the related pathologies including atherosclerosis, cancer, and rheumatoid arthritis. We examine the pros and cons of anti-angiogenic therapies currently in clinical trials. We also reveal novel therapeutic strategies that cause broad-spectrum inhibition of the CC-chemokine class that may have future potential for the specific inhibition of inflammatory angiogenesis. Full article

(This article belongs to the Special Issue Vascular Biology and Therapeutics)

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10 pages, 3465 KiB

Open AccessArticle

Systematic Analysis of Protein Interaction Network Associated with Azoospermia

by Soudabeh Sabetian^* and Mohd Shahir Shamsir

Department of Biological and Health Sciences, Faculty of Bioscience & Medical Engineering, Universiti Teknologi Malaysia, 81310 Johor, Malaysia

Int. J. Mol. Sci. 2016, 17(11), 1857; https://doi.org/10.3390/ijms17111857 - 10 Nov 2016

Cited by 14 | Viewed by 5631

Abstract

Non-obstructive azoospermia is a severe infertility factor. Currently, the etiology of this condition remains elusive with several possible molecular pathway disruptions identified in the post-meiotic spermatozoa. In the presented study, in order to identify all possible candidate genes associated with azoospermia and to [...] Read more.

Non-obstructive azoospermia is a severe infertility factor. Currently, the etiology of this condition remains elusive with several possible molecular pathway disruptions identified in the post-meiotic spermatozoa. In the presented study, in order to identify all possible candidate genes associated with azoospermia and to map their relationship, we present the first protein-protein interaction network related to azoospermia and analyze the complex effects of the related genes systematically. Using Online Mendelian Inheritance in Man, the Human Protein Reference Database and Cytoscape, we created a novel network consisting of 209 protein nodes and 737 interactions. Mathematical analysis identified three proteins, ar, dazap2, and esr1, as hub nodes and a bottleneck protein within the network. We also identified new candidate genes, CREBBP and BCAR1, which may play a role in azoospermia. The gene ontology analysis suggests a genetic link between azoospermia and liver disease. The KEGG analysis also showed 45 statistically important pathways with 31 proteins associated with colorectal, pancreatic, chronic myeloid leukemia and prostate cancer. Two new genes and associated diseases are promising for further experimental validation. Full article

(This article belongs to the Special Issue Proteins and Protein-Ligand Interactions)

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9 pages, 882 KiB

Open AccessReview

The Multifaceted Roles of PI3Kγ in Hypertension, Vascular Biology, and Inflammation

by Marialuisa Perrotta¹, Giuseppe Lembo^1,2 and Daniela Carnevale^1,2,*

¹ Department of Angiocardioneurology and Translational Medicine, IRCCS Neuromed, 86077 Pozzilli, Italy

² Department of Molecular Medicine, Sapienza University of Rome, 00161 Rome, Italy

Int. J. Mol. Sci. 2016, 17(11), 1858; https://doi.org/10.3390/ijms17111858 - 8 Nov 2016

Cited by 15 | Viewed by 6522

Abstract

PI3Kγ is a multifaceted protein, crucially involved in cardiovascular and immune systems. Several studies described the biological and physiological functions of this enzyme in the regulation of cardiovascular system, while others stressed its role in the modulation of immunity. Although PI3Kγ has been [...] Read more.

PI3Kγ is a multifaceted protein, crucially involved in cardiovascular and immune systems. Several studies described the biological and physiological functions of this enzyme in the regulation of cardiovascular system, while others stressed its role in the modulation of immunity. Although PI3Kγ has been historically investigated for its role in leukocytes, the last decade of research also dedicated efforts to explore its functions in the cardiovascular system. In this review, we report an overview recapitulating how PI3Kγ signaling participates in the regulation of vascular functions involved in blood pressure regulation. Moreover, we also summarize the main functions of PI3Kγ in immune responses that could be potentially important in the interaction with the cardiovascular system. Considering that vascular and immune mechanisms are increasingly emerging as intertwining players in hypertension, PI3Kγ could be an intriguing pathway acting on both sides. The availability of specific inhibitors introduces a perspective of further translational research and clinical approaches that could be exploited in hypertension. Full article

(This article belongs to the Special Issue Vascular Biology and Therapeutics)

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11 pages, 5680 KiB

Open AccessArticle

Low Expression of CAPON in Glioma Contributes to Cell Proliferation via the Akt Signaling Pathway

by Shangfeng Gao^1,2,†, Jie Wang^2,†, Tong Zhang², Guangping Liu³, Lei Jin², Daofei Ji², Peng Wang², Qingming Meng^1,2, Yufu Zhu^1,2,* and Rutong Yu^1,2,*

¹ Institute of Nervous System Diseases, Xuzhou Medical University, 84 West Huai-Hai Road, Xuzhou 221002, Jiangsu, China

² Brain Hospital, Affiliated Hospital of Xuzhou Medical University, 99 West Huai-Hai Road, Xuzhou 221002, Jiangsu, China

³ People’s Hospital of Juxian, 100 Fulaizhong Road in Juxian, Rizhao 276500, Shandong, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1859; https://doi.org/10.3390/ijms17111859 - 18 Nov 2016

Cited by 12 | Viewed by 5688

Abstract

CAPON is an adapter protein for nitric oxide synthase 1 (NOS1). CAPON has two isoforms in the human brain: CAPON-L (long form of CAPON) and CAPON-S (short form of CAPON). Recent studies have indicated the involvement of CAPON in tumorigenesis beyond its classical [...] Read more.

CAPON is an adapter protein for nitric oxide synthase 1 (NOS1). CAPON has two isoforms in the human brain: CAPON-L (long form of CAPON) and CAPON-S (short form of CAPON). Recent studies have indicated the involvement of CAPON in tumorigenesis beyond its classical role in NOS1 activity regulation. In this study, we found that the protein levels of CAPON-S, but not than CAPON-L, were significantly decreased in glioma tissues. Therefore, we established lentivirus-mediated stable cell lines with CAPON-S overexpression or down-regulation, and investigated the role of CAPON-S in the proliferation of glioma cells by using CCK8, EdU, and flow cytometry assays. Overexpression of CAPON-S reduced the cell variability and the percentage of EdU-positive cells, and arrested the cells in the G1 phase in glioma cells. Silencing of CAPON by short-hairpin RNA showed the opposite effects. Furthermore, an intracellular signaling array revealed that overexpression of CAPON-S resulted in a remarkable reduction in the phosphorylation of Akt and S6 ribosomal protein in glioma cells, which was further confirmed by Western blot. These findings suggest that CAPON may function as a tumor suppressor in human brain glioma and that the inactivation of the Akt signaling pathway caused by CAPON-S overexpression may provide insight into the underlying mechanism of CAPON in glioma cell proliferation. Full article

(This article belongs to the Special Issue Advances in Molecular Oncology)

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10 pages, 572 KiB

Open AccessReview

Focal Adhesion Kinase-Dependent Role of the Soluble Form of Neurotensin Receptor-3/Sortilin in Colorectal Cancer Cell Dissociation

by Sophie Béraud-Dufour, Christelle Devader

, Fabienne Massa, Morgane Roulot, Thierry Coppola and Jean Mazella^*

Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR 7275, Université Côte d’Azur, 660 route des Lucioles, 06560 Valbonne, France

Int. J. Mol. Sci. 2016, 17(11), 1860; https://doi.org/10.3390/ijms17111860 - 8 Nov 2016

Cited by 20 | Viewed by 6414

Abstract

The aim of the present review is to unravel the mechanisms of action of the soluble form of the neurotensin (NT) receptor-3 (NTSR3), also called Sortilin, in numerous physiopathological processes including cancer development, cardiovascular diseases and depression. Sortilin/NTSR3 is a transmembrane protein thought [...] Read more.

The aim of the present review is to unravel the mechanisms of action of the soluble form of the neurotensin (NT) receptor-3 (NTSR3), also called Sortilin, in numerous physiopathological processes including cancer development, cardiovascular diseases and depression. Sortilin/NTSR3 is a transmembrane protein thought to exert multiple functions both intracellularly and at the level of the plasma membrane. The Sortilin/NTSR3 extracellular domain is released by shedding from all the cells expressing the protein. Although the existence of the soluble form of Sortilin/NTSR3 (sSortilin/NTSR3) has been evidenced for more than 10 years, the studies focusing on the role of this soluble protein at the mechanistic level remain rare. Numerous cancer cells, including colonic cancer cells, express the receptor family of neurotensin (NT), and particularly Sortilin/NTSR3. This review aims to summarize the functional role of sSortilin/NTSR3 characterized in the colonic cancer cell line HT29. This includes mechanisms involving signaling cascades through focal adhesion kinase (FAK), a key pathway leading to the weakening of cell–cell and cell–extracellular matrix adhesions, a series of events which could be responsible for cancer metastasis. Finally, some future approaches targeting the release of sNTSR3 through the inhibition of matrix metalloproteases (MMPs) are suggested. Full article

(This article belongs to the Special Issue Kinase Signal Transduction)

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10 pages, 482 KiB

Open AccessArticle

Association between Interleukin-6 Promoter Polymorphism (-174 G/C), Serum Interleukin-6 Levels and Mortality in Severe Septic Patients

by Leonardo Lorente^1,*

, María M. Martín², Antonia Pérez-Cejas³, Ysamar Barrios⁴, Jordi Solé-Violán⁵

, José Ferreres⁶, Lorenzo Labarta⁷, César Díaz⁸ and Alejandro Jiménez⁹

¹ Intensive Care Unit, Hospital Universitario de Canarias, Ofra, s/n, La Laguna, 38320 Santa Cruz de Tenerife, Spain

² Intensive Care Unit, Hospital Universitario Nuestra Señora Candelaria, Crta Rosario s/n, 38010 Santa Cruz de Tenerife, Spain

³ Laboratory Deparment, Hospital Universitario de Canarias, Ofra, s/n, La Laguna, 38320 Santa Cruz de Tenerife, Spain

⁴ Research Unit, Hospital Universitario de Canarias, Ofra, s/n, La Laguna, 38320 Santa Cruz de Tenerife, Spain

⁵ Intensive Care Unit, Hospital Universitario Dr. Negrín, Barranco de la Ballena s/n, 35010 Las Palmas de Gran Canaria, Spain

⁶ Intensive Care Unit, Hospital Clínico Universitario de Valencia, Avda, Blasco Ibáñez nº17, 46004 Valencia, Spain

⁷ Intensive Care Unit, Hospital San Jorge de Huesca, Avenida Martínez de Velasco nº36, 22004 Huesca, Spain

⁸ Intensive Care Unit, Hospital Insular, Plaza Dr. Pasteur s/n, 35016 Las Palmas de Gran Canaria, Spain

⁹ Research Unit, Hospital Universitario de Canarias, Ofra, s/n, La Laguna-38320, 38320 Santa Cruz de Tenerife, Spain

Int. J. Mol. Sci. 2016, 17(11), 1861; https://doi.org/10.3390/ijms17111861 - 8 Nov 2016

Cited by 20 | Viewed by 5081

Abstract

The association between interleukin (IL)-6 promoter polymorphism (-174 G/C), circulating IL-6 levels and mortality in septic patients has scarcely been addressed, and then only in studies of small sample size, and a direct association among them has not been previously reported. Therefore, the [...] Read more.

The association between interleukin (IL)-6 promoter polymorphism (-174 G/C), circulating IL-6 levels and mortality in septic patients has scarcely been addressed, and then only in studies of small sample size, and a direct association among them has not been previously reported. Therefore, the purpose of our study was to determine whether this association exists. An observational, prospective and multicenter study including severe septic patients was undertaken and serum IL-6 levels at severe sepsis diagnosis and IL-6 promoter polymorphism (-174 G/C) were determined. The end-point of the study was 30-day mortality. The study included 263 patients with the following genotypes of IL-6 promoter polymorphism (-174 G/C): 123 (46.8%) GG, 110 (41.8%) GC and 30 (11.4%) CC. CC homozygous patients showed lower sepsis-related organ failure assessment (SOFA) score, serum IL-6 levels and mortality at 30 days compared to those with other genotypes (GC or GG). On regression analysis, CC homozygous patients showed lower 30-day mortality than those with genotype GG (odds ratio = 0.21; 95% CI = 0.053−0.838; p = 0.03) or GC (hazard ratio = 0.28; 95% CI = 0.074−1.037; p = 0.06). The most important results of our study were that CC might be a favorable genotype in septic patients showing lower serum IL-6 levels and lower risk of death within 30 days. Full article

(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)

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8 pages, 1733 KiB

Open AccessArticle

Classification of 27 Tumor-Associated Antigens by Histochemical Analysis of 36 Freshly Resected Lung Cancer Tissues

by Gene Kurosawa¹

, Mototaka Sugiura², Yoshinobu Hattori^3,5, Hiroyuki Tsuda⁴ and Yoshikazu Kurosawa^1,*

¹ Division of Antibody Project, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, Japan

² Internal Medicine, School of Medicine, Fujita Health University, Toyoake 470-1192, Japan

³ Division of Thoracic and Cardiovascular Surgery, School of Medicine, Fujita Health University, Toyoake 470-1192, Japan

⁴ Department of Toxicology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8603, Japan

⁵ Department of Surgery, Social Medical Corporation Kojunkai Daido Hospital, Nagoya 457-8511, Japan

Int. J. Mol. Sci. 2016, 17(11), 1862; https://doi.org/10.3390/ijms17111862 - 8 Nov 2016

Cited by 6 | Viewed by 4901

Abstract

In previous studies, we identified 29 tumor-associated antigens (TAAs) and isolated 488 human monoclonal antibodies (mAbs) that specifically bind to one of the 29 TAAs. In the present study, we performed histochemical analysis of 36 freshly resected lung cancer tissues by using 60 [...] Read more.

In previous studies, we identified 29 tumor-associated antigens (TAAs) and isolated 488 human monoclonal antibodies (mAbs) that specifically bind to one of the 29 TAAs. In the present study, we performed histochemical analysis of 36 freshly resected lung cancer tissues by using 60 mAbs against 27 TAAs. Comparison of the staining patterns of tumor cells, bronchial epithelial cells, and normal pulmonary alveolus cells and interalveolar septum allowed us to determine the type and location of cells that express target molecules, as well as the degree of expression. The patterns were classified into 7 categories. While multiple Abs were used against certain TAAs, the differences observed among them should be derived from differences in the binding activity and/or the epitope. Thus, such data indicate the versatility of respective clones as anti-cancer drugs. Although the information obtained was limited to the lung and bronchial tube, bronchial epithelial cells represent normal growing cells, and therefore, the data are informative. The results indicate that 9 of the 27 TAAs are suitable targets for therapeutic Abs. These 9 Ags include EGFR, HER2, TfR, and integrin α6β4. Based on our findings, a pharmaceutical company has started to develop anti-cancer drugs by using Abs to TfR and integrin α6β4. HGFR, PTP-LAR, CD147, CDCP1, and integrin αvβ3 are also appropriate targets for therapeutic purposes. Full article

(This article belongs to the Special Issue Molecular Classification of Human Cancer: Diagnosis and Treatment 2016)

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14 pages, 1903 KiB

Open AccessArticle

Negative Glucocorticoid Response-Like Element from the First Intron of the Chicken Growth Hormone Gene Represses Gene Expression in the Rat Pituitary Tumor Cell Line

by Jing-E. Ma^1,2,3

, Qian-Qian Lang^1,2,3, Feng-Fang Qiu^1,2,3, Li Zhang^1,2,3, Xiang-Guang Li^1,2, Wen Luo^1,2,3, Juan Wang^1,2,3, Xing Wang^1,2,3, Xi-Ran Lin^1,2,3, Wen-Sheng Liu^1,2,3, Qing-Hua Nie^1,2,3

and Xi-Quan Zhang^1,2,3,*

¹ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

² Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China

³ Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China

Int. J. Mol. Sci. 2016, 17(11), 1863; https://doi.org/10.3390/ijms17111863 - 9 Nov 2016

Cited by 2 | Viewed by 5272

Abstract

The effects of introns, especially the first intron, on the regulation of gene expression remains unclear. Therefore, the objective of the present study was to investigate the transcriptional regulatory function of intron 1 on the chicken growth hormone (cGH) gene in [...] Read more.

The effects of introns, especially the first intron, on the regulation of gene expression remains unclear. Therefore, the objective of the present study was to investigate the transcriptional regulatory function of intron 1 on the chicken growth hormone (cGH) gene in the rat pituitary tumor cell line (GH4-C1). Transient transfection using first-intron-inserted cGH complete coding sequences (CDSs) and non-intron-inserted cGH CDS plasmids, quantitative RT-PCR (qRT-PCR) and western blot assays were used to detect the expression of cGH. The reporter gene assay was also used to investigate the effect of a series of fragments in the first intron of cGH on gene expression in GH4-C1. All of the results revealed that a 200-bp fragment located in the +485/+684 region of intron 1 was essential for repressing the expression of cGH. Further informatics analysis showed that there was a cluster of 13 transcriptional factor binding sites (TFBSs) in the +485/+684 region of the cGH intron 1. Disruption of a glucocorticoid response-like element (the 19-nucleotide sequence 5′-AGGCTTGACAGTGACCTCC-3′) containing a T-box motif (TGACCT) located within this DNA fragment increased the expression of the reporter gene in GH4-C1. In addition, an electrophoretic mobility shift assay (EMSA) revealed a glucocorticoid receptor (GR) protein of rat binding to the glucocorticoid response-like element. Together, these results indicate that there is a negative glucocorticoid response-like element (nGRE) located in the +591/+609 region within the first intron of cGH, which is essential for the down-regulation of cGH expression. Full article

(This article belongs to the Section Biochemistry)

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11 pages, 229 KiB

Open AccessReview

Impact of Microbes on the Pathogenesis of Primary Biliary Cirrhosis (PBC) and Primary Sclerosing Cholangitis (PSC)

by Jochen Mattner

Mikrobiologisches Institut—Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen and Friedrich-Alexander Universität (FAU) Erlangen-Nürnberg, Wasserturmstr. 3/5, D-91054 Erlangen, Germany

Int. J. Mol. Sci. 2016, 17(11), 1864; https://doi.org/10.3390/ijms17111864 - 9 Nov 2016

Cited by 46 | Viewed by 11319

Abstract

Primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC) represent the major clinical entities of chronic cholestatic liver diseases. Both disorders are characterized by portal inflammation and slowly progress to obliterative fibrosis and eventually liver cirrhosis. Although immune-pathogenic mechanisms have been implicated in [...] Read more.

Primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC) represent the major clinical entities of chronic cholestatic liver diseases. Both disorders are characterized by portal inflammation and slowly progress to obliterative fibrosis and eventually liver cirrhosis. Although immune-pathogenic mechanisms have been implicated in the pathogenesis of PBC and PSC, neither disorder is considered to be a classical autoimmune disease, as PSC and PBC patients do not respond to immune-suppressants. Furthermore, the decreased bile flow resulting from the immune-mediated tissue assault and the subsequent accumulation of toxic bile products in PBC and PSC not only perpetuates biliary epithelial damage, but also alters the composition of the intestinal and biliary microbiota and its mutual interactions with the host. Consistent with the close association of PSC and inflammatory bowel disease (IBD), the polyclonal hyper IgM response in PBC and (auto-)antibodies which cross-react to microbial antigens in both diseases, an expansion of individual microbes leads to shifts in the composition of the intestinal or biliary microbiota and a subsequent altered integrity of epithelial layers, promoting microbial translocation. These changes have been implicated in the pathogenesis of both devastating disorders. Thus, we will discuss here these recent findings in the context of novel and alternative therapeutic options. Full article

(This article belongs to the Special Issue Molecular Mechanisms of Human Liver Diseases)

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12 pages, 9421 KiB

Open AccessArticle

Berberine Alleviates Olanzapine-Induced Adipogenesis via the AMPKα–SREBP Pathway in 3T3-L1 Cells

by Yanjie Li^1,2, Xiaomin Zhao^1,2, Xiyu Feng^1,2, Xuemei Liu^1,2, Chao Deng³

and Chang-Hua Hu^1,2,*

¹ School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

² Engineer Research Center of Chongqing Pharmaceutical Process and Quality Control, Chongqing 400715, China

³ Antipsychotic Research Laboratory, School of Medicine, and Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong 2522, NSW, Australia

Int. J. Mol. Sci. 2016, 17(11), 1865; https://doi.org/10.3390/ijms17111865 - 9 Nov 2016

Cited by 51 | Viewed by 10693

Abstract

The aim of this study was to investigate the mechanisms underlying the inhibitory effects of berberine (BBR) on olanzapine (OLZ)-induced adipogenesis in a well-replicated 3T3-L1 cell model. Oil-Red-O (ORO) staining showed that BBR significantly decreased OLZ-induced adipogenesis. Co-treatment with OLZ and BBR decreased [...] Read more.

The aim of this study was to investigate the mechanisms underlying the inhibitory effects of berberine (BBR) on olanzapine (OLZ)-induced adipogenesis in a well-replicated 3T3-L1 cell model. Oil-Red-O (ORO) staining showed that BBR significantly decreased OLZ-induced adipogenesis. Co-treatment with OLZ and BBR decreased the accumulation of triglyceride (TG) and total cholesterol (TC) by 55.58% ± 3.65% and 49.84% ± 8.31%, respectively, in 3T3-L1 adipocytes accompanied by reduced expression of Sterol regulatory element binding proteins 1 (SREBP1), fatty acid synthase (FAS), peroxisome proliferator activated receptor-γ (PPARγ), SREBP2, low-density lipoprotein receptor (LDLR), and hydroxymethylglutaryl-coenzyme A reductase (HMGR) genes compared with OLZ alone. Consistently, the co-treatment downregulated protein levels of SREBP1, SREBP2, and LDLR by 57.71% ± 9.42%, 73.05% ± 11.82%, and 59.46% ± 9.91%, respectively. In addition, co-treatment reversed the phosphorylation level of AMP-activated protein kinase-α (AMPKα), which was reduced by OLZ, determined via the ratio of pAMPKα:AMPKα (94.1%) compared with OLZ alone. The results showed that BBR may prevent lipid metabolism disorders caused by OLZ by reversing the degree of SREBP pathway upregulated and the phosphorylation of AMPKα downregulated. Collectively, these results indicated that BBR could be used as a potential adjuvant to prevent dyslipidemia and obesity caused by the use of second-generation antipsychotic medication. Full article

(This article belongs to the Section Bioactives and Nutraceuticals)

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17 pages, 2244 KiB

Open AccessArticle

Pharmacologic Treatment Assigned for Niemann Pick Type C1 Disease Partly Changes Behavioral Traits in Wild-Type Mice

by Victoria Schlegel^1,†, Markus Thieme^1,†, Carsten Holzmann²

, Martin Witt¹

, Ulrike Grittner³

, Arndt Rolfs⁴ and Andreas Wree^1,*

¹ Institute of Anatomy, University of Rostock, 18055 Rostock, Germany

² Institute of Medical Genetics, Rostock University Medical Center, 18057 Rostock, Germany

³ Department for Biostatistics and Clinical Epidemiology, Charité—Universitätsmedizin Berlin, 10117 Berlin, Germany

⁴ Albrecht-Kossel Institute for Neuroregeneration, Rostock University Medical Center, 18147 Rostock, Germany

^† Authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1866; https://doi.org/10.3390/ijms17111866 - 9 Nov 2016

Cited by 16 | Viewed by 6418

Abstract

Niemann-Pick Type C1 (NPC1) is an autosomal recessive inherited disorder characterized by accumulation of cholesterol and glycosphingolipids. Previously, we demonstrated that BALB/c-npc1^nihNpc1^−/− mice treated with miglustat, cyclodextrin and allopregnanolone generally performed better than untreated Npc1^−/− animals. Unexpectedly, they also [...] Read more.

Niemann-Pick Type C1 (NPC1) is an autosomal recessive inherited disorder characterized by accumulation of cholesterol and glycosphingolipids. Previously, we demonstrated that BALB/c-npc1^nihNpc1^−/− mice treated with miglustat, cyclodextrin and allopregnanolone generally performed better than untreated Npc1^−/− animals. Unexpectedly, they also seemed to accomplish motor tests better than their sham-treated wild-type littermates. However, combination-treated mutant mice displayed worse cognition performance compared to sham-treated ones. To evaluate effects of these drugs in healthy BALB/c mice, we here analyzed pharmacologic effects on motor and cognitive behavior of wild-type mice. For combination treatment mice were injected with allopregnanolone/cyclodextrin weekly, starting at P7. Miglustat injections were performed daily from P10 till P23. Starting at P23, miglustat was embedded in the chow. Other mice were treated with miglustat only, or sham-treated. The battery of behavioral tests consisted of accelerod, Morris water maze, elevated plus maze, open field and hot-plate tests. Motor capabilities and spontaneous motor behavior were unaltered in both drug-treated groups. Miglustat-treated wild-type mice displayed impaired spatial learning compared to sham- and combination-treated mice. Both combination- and miglustat-treated mice showed enhanced anxiety in the elevated plus maze compared to sham-treated mice. Additionally, combination treatment as well as miglustat alone significantly reduced brain weight, whereas only combination treatment reduced body weight significantly. Our results suggest that allopregnanolone/cyclodextrin ameliorate most side effects of miglustat in wild-type mice. Full article

(This article belongs to the Special Issue Lysosomal Storage Disorders: Novel Concepts, Therapeutic Aspects and Beyond)

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19 pages, 6907 KiB

Open AccessArticle

Tonsil-Derived Mesenchymal Stem Cells Differentiate into a Schwann Cell Phenotype and Promote Peripheral Nerve Regeneration

by Namhee Jung¹

, Saeyoung Park¹, Yoonyoung Choi¹, Joo-Won Park¹, Young Bin Hong², Hyun Ho Choi Park³, Yeonsil Yu⁴, Geon Kwak⁵, Han Su Kim⁶, Kyung-Ha Ryu⁷, Jae Kwang Kim⁸, Inho Jo⁴

, Byung-Ok Choi⁹ and Sung-Chul Jung^1,*

¹ Department of Biochemistry, School of Medicine, Ewha Womans University, Seoul 07985, Korea

² Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul 06351, Korea

³ Department of Neuroscience and Behavioral Biology, Emory University, Atlanta, GA 30322, USA

⁴ Department of Molecular Medicine, School of Medicine, Ewha Womans University, Seoul 07985, Korea

⁵ Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Korea

⁶ Department of Otorhinolaryngology-Head & Neck Surgery, School of Medicine, Ewha Womans University, Seoul 07985, Korea

⁷ Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul 07985, Korea

⁸ Department of Orthopedic Surgery, School of Medicine, Ewha Womans University, Seoul 07985, Korea

⁹ Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea

Int. J. Mol. Sci. 2016, 17(11), 1867; https://doi.org/10.3390/ijms17111867 - 9 Nov 2016

Cited by 56 | Viewed by 9602

Abstract

Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been [...] Read more.

Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been performed and has shown promising clinical results for treating nerve injuries and donor site morbidity, and insufficient production of the cells have been considered as a major issue. Here, we performed differentiation of tonsil-derived mesenchymal stem cells (T-MSCs) into SC-like cells (T-MSC-SCs), to evaluate T-MSC-SCs as an alternative to SCs. Using SC markers such as CAD19, GFAP, MBP, NGFR, S100B, and KROX20 during quantitative real-time PCR we detected the upregulation of NGFR, S100B, and KROX20 and the downregulation of CAD19 and MBP at the fully differentiated stage. Furthermore, we found myelination of axons when differentiated SCs were cocultured with mouse dorsal root ganglion neurons. The application of T-MSC-SCs to a mouse model of sciatic nerve injury produced marked improvements in gait and promoted regeneration of damaged nerves. Thus, the transplantation of human T-MSCs might be suitable for assisting in peripheral nerve regeneration. Full article

(This article belongs to the Special Issue Peripheral Nerve Regeneration: From Bench to Bedside)

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13 pages, 5255 KiB

Open AccessArticle

Stearoyl-CoA Desaturase-1 Protects Cells against Lipotoxicity-Mediated Apoptosis in Proximal Tubular Cells

by Tamaki Iwai¹, Shinji Kume^1,*

, Masami Chin-Kanasaki¹, Shogo Kuwagata¹, Hisazumi Araki¹, Naoko Takeda¹, Takeshi Sugaya², Takashi Uzu¹, Hiroshi Maegawa¹ and Shin-ichi Araki^1,*

¹ Department of Medicine, Shiga University of Medical Science, Tsukinowa-cho, Seta, Otsu, Shiga 520-2192, Japan

² Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan

Int. J. Mol. Sci. 2016, 17(11), 1868; https://doi.org/10.3390/ijms17111868 - 9 Nov 2016

Cited by 46 | Viewed by 7695

Abstract

Saturated fatty acid (SFA)-related lipotoxicity is a pathogenesis of diabetes-related renal proximal tubular epithelial cell (PTEC) damage, closely associated with a progressive decline in renal function. This study was designed to identify a free fatty acid (FFA) metabolism-related enzyme that can protect PTECs [...] Read more.

Saturated fatty acid (SFA)-related lipotoxicity is a pathogenesis of diabetes-related renal proximal tubular epithelial cell (PTEC) damage, closely associated with a progressive decline in renal function. This study was designed to identify a free fatty acid (FFA) metabolism-related enzyme that can protect PTECs from SFA-related lipotoxicity. Among several enzymes involved in FFA metabolism, we identified stearoyl-CoA desaturase-1 (SCD1), whose expression level significantly decreased in the kidneys of high-fat diet (HFD)-induced diabetic mice, compared with non-diabetic mice. SCD1 is an enzyme that desaturates SFAs, converting them to monounsaturated fatty acids (MUFAs), leading to the formation of neutral lipid droplets. In culture, retrovirus-mediated overexpression of SCD1 or MUFA treatment significantly ameliorated SFA-induced apoptosis in PTECs by enhancing intracellular lipid droplet formation. In contrast, siRNA against SCD1 exacerbated the apoptosis. Both overexpression of SCD1 and MUFA treatment reduced SFA-induced apoptosis via reducing endoplasmic reticulum stress in cultured PTECs. Thus, HFD-induced decrease in renal SCD1 expression may play a pathogenic role in lipotoxicity-induced renal injury, and enhancing SCD1-mediated desaturation of SFA and subsequent formation of neutral lipid droplets may become a promising therapeutic target to reduce SFA-induced lipotoxicity. The present study provides a novel insight into lipotoxicity in the pathogenesis of diabetic nephropathy. Full article

(This article belongs to the Special Issue Diabetic Complications: Pathophysiology, Mechanisms, and Therapies)

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15 pages, 4502 KiB

Open AccessArticle

Brefeldin A-Inhibited Guanine Nucleotide-Exchange Factor 1 (BIG1) Governs the Recruitment of Tumor Necrosis Factor Receptor-Associated Factor 2 (TRAF2) to Tumor Necrosis Factor Receptor 1 (TNFR1) Signaling Complexes

by Takuya Noguchi^1,2,*,†

, Mei Tsuchida^2,†, Yosuke Kogue^2,†, Christian Spadini¹, Yusuke Hirata² and Atsushi Matsuzawa^2,*

¹ Department of Biochemistry, University of Lausanne, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland

² Laboratory of Health Chemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, 980-8578 Sendai, Japan

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1869; https://doi.org/10.3390/ijms17111869 - 9 Nov 2016

Cited by 17 | Viewed by 7474

Abstract

Tumor necrosis factor receptor-associated factor 2 (TRAF2) is a critical mediator of tumor necrosis factor-α (TNF-α) signaling. However, the regulatory mechanisms of TRAF2 are not fully understood. Here we show evidence that TRAF2 requires brefeldin A-inhibited guanine nucleotide-exchange factor 1 (BIG1) to be [...] Read more.

Tumor necrosis factor receptor-associated factor 2 (TRAF2) is a critical mediator of tumor necrosis factor-α (TNF-α) signaling. However, the regulatory mechanisms of TRAF2 are not fully understood. Here we show evidence that TRAF2 requires brefeldin A-inhibited guanine nucleotide-exchange factor 1 (BIG1) to be recruited into TNF receptor 1 (TNFR1) signaling complexes. In BIG1 knockdown cells, TNF-α-induced c-Jun N-terminal kinase (JNK) activation was attenuated and the sensitivity to TNF-α-induced apoptosis was increased. Since these trends correlated well with those of TRAF2 deficient cells as previously demonstrated, we tested whether BIG1 functions as an upstream regulator of TRAF2 in TNFR1 signaling. As expected, we found that knockdown of BIG1 suppressed TNF-α-dependent ubiquitination of TRAF2 that is required for JNK activation, and impaired the recruitment of TRAF2 to the TNFR1 signaling complex (complex I). Moreover, we found that the recruitment of TRAF2 to the death-inducing signaling complex termed complex II was also impaired in BIG1 knockdown cells. These results suggest that BIG1 is a key component of the machinery that drives TRAF2 to the signaling complexes formed after TNFR1 activation. Thus, our data demonstrate a novel and unexpected function of BIG1 that regulates TNFR1 signaling by targeting TRAF2. Full article

(This article belongs to the Special Issue Kinase Signal Transduction)

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12 pages, 2701 KiB

Open AccessArticle

A Precision Microbiome Approach Using Sucrose for Selective Augmentation of Staphylococcus epidermidis Fermentation against Propionibacterium acnes

by Yanhan Wang¹

, Ming-Shan Kao², Jinghua Yu³, Stephen Huang⁴, Shinta Marito², Richard L. Gallo¹ and Chun-Ming Huang^1,5,*

¹ Department of Dermatology, School of Medicine, University of California, San Diego, CA 92093, USA

² Department of Biomedical Sciences and Engineering, National Central University, Taoyuan 320009, Taiwan

³ NMR and Crystallography Facilities, Sanford-Burnham Institute for Medical Research, La Jolla, CA 92037, USA

⁴ Surface Bioadvances Inc., San Diego, CA 92121, USA

⁵ Moores Cancer Center, University of California, San Diego, CA 92103, USA

Int. J. Mol. Sci. 2016, 17(11), 1870; https://doi.org/10.3390/ijms17111870 - 9 Nov 2016

Cited by 59 | Viewed by 16877

Abstract

Acne dysbiosis happens when there is a microbial imbalance of the over-growth of Propionibacterium acnes (P. acnes) in the acne microbiome. In our previous study, we demonstrated that Staphylococcus epidermidis (S. epidermidis, a probiotic skin bacterium) can exploit [...] Read more.

Acne dysbiosis happens when there is a microbial imbalance of the over-growth of Propionibacterium acnes (P. acnes) in the acne microbiome. In our previous study, we demonstrated that Staphylococcus epidermidis (S. epidermidis, a probiotic skin bacterium) can exploit glycerol fermentation to produce short-chain fatty acids (SCFAs) which have antimicrobial activities to suppress the growth of P. acnes. Unlike glycerol, sucrose is chosen here as a selective fermentation initiator (SFI) that can specifically intensify the fermentation activity of S. epidermidis, but not P. acnes. A co-culture of P. acnes and fermenting S. epidermidis in the presence of sucrose significantly led to a reduction in the growth of P. acnes. The reduction was abolished when P. acnes was co-cultured with non-fermenting S. epidermidis. Results from nuclear magnetic resonance (NMR) analysis revealed four SCFAs (acetic acid, butyric acid, lactic acid, and succinic acid) were detectable in the media of S. epidermidis sucrose fermentation. To validate the interference of S. epidermidis sucrose fermentation with P. acnes, mouse ears were injected with both P. acnes and S. epidermidis plus sucrose or phosphate buffered saline (PBS). The level of macrophage-inflammatory protein-2 (MIP-2) and the number of P. acnes in ears injected with two bacteria plus sucrose were considerably lower than those in ears injected with two bacteria plus PBS. Our results demonstrate a precision microbiome approach by using sucrose as a SFI for S. epidermidis, holding future potential as a novel modality to equilibrate dysbiotic acne. Full article

(This article belongs to the Special Issue Inflammatory Skin Conditions)

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15 pages, 699 KiB

Open AccessReview

Metabolomics, a Powerful Tool for Agricultural Research

by He Tian, Sin Man Lam and Guanghou Shui^*

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Int. J. Mol. Sci. 2016, 17(11), 1871; https://doi.org/10.3390/ijms17111871 - 17 Nov 2016

Cited by 74 | Viewed by 8422

Abstract

Metabolomics, which is based mainly on nuclear magnetic resonance (NMR), gas-chromatography (GC) or liquid-chromatography (LC) coupled to mass spectrometry (MS) analytical technologies to systematically acquire the qualitative and quantitative information of low-molecular-mass endogenous metabolites, provides a direct snapshot of the physiological condition in [...] Read more.

Metabolomics, which is based mainly on nuclear magnetic resonance (NMR), gas-chromatography (GC) or liquid-chromatography (LC) coupled to mass spectrometry (MS) analytical technologies to systematically acquire the qualitative and quantitative information of low-molecular-mass endogenous metabolites, provides a direct snapshot of the physiological condition in biological samples. As complements to transcriptomics and proteomics, it has played pivotal roles in agricultural and food science research. In this review, we discuss the capacities of NMR, GC/LC-MS in the acquisition of plant metabolome, and address the potential promise and diverse applications of metabolomics, particularly lipidomics, to investigate the responses of Arabidopsis thaliana, a primary plant model for agricultural research, to environmental stressors including heat, freezing, drought, and salinity. Full article

(This article belongs to the Special Issue Lipidomics and Glycomics: New Advances in Food Science and Nutrition)

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23 pages, 1992 KiB

Open AccessArticle

Response Surface Methodology Modelling of an Aqueous Two-Phase System for Purification of Protease from Penicillium candidum (PCA 1/TT031) under Solid State Fermentation and Its Biochemical Characterization

by Amaal M. Alhelli^1,2, Mohd Yazid Abdul Manap^1,3, Abdulkarim Sabo Mohammed¹, Hamed Mirhosseini¹, Eilaf Suliman¹

, Zahra Shad¹, Nameer Khairulla Mohammed¹ and Anis Shobirin Meor Hussin^1,3,*

¹ Faculty of Food Science and Technology, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

² Institute of technology, Middle technical University, 29008 Alzafaranya, Baghdad, Iraq

³ Halal Products Research Institute, University Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

Int. J. Mol. Sci. 2016, 17(11), 1872; https://doi.org/10.3390/ijms17111872 - 11 Nov 2016

Cited by 21 | Viewed by 6045

Abstract

Penicillium candidum (PCA 1/TT031) synthesizes different types of extracellular proteases. The objective of this study is to optimize polyethylene glycol (PEG)/citrate based on an aqueous two-phase system (ATPS) and Response Surface Methodology (RSM) to purify protease from Penicillium candidum (PCA 1/TT031). The effects [...] Read more.

Penicillium candidum (PCA 1/TT031) synthesizes different types of extracellular proteases. The objective of this study is to optimize polyethylene glycol (PEG)/citrate based on an aqueous two-phase system (ATPS) and Response Surface Methodology (RSM) to purify protease from Penicillium candidum (PCA 1/TT031). The effects of different PEG molecular weights (1500–10,000 g/mol), PEG concentration (9%–20%), concentrations of NaCl (0%–10%) and the citrate buffer (8%–16%) on protease were also studied. The best protease purification could be achieved under the conditions of 9.0% (w/w) PEG 8000, 5.2% NaCl, and 15.9% sodium citrate concentration, which resulted in a one-sided protease partitioning for the bottom phase with a partition coefficient of 0.2, a 6.8-fold protease purification factor, and a yield of 93%. The response surface models displayed a significant (p ≤ 0.05) response which was fit for the variables that were studied as well as a high coefficient of determination (R²). Similarly, the predicted and observed values displayed no significant (p > 0.05) differences. In addition, our enzyme characterization study revealed that Penicillium candidum (PCA 1/TT031) produced a slight neutral protease with a molecular weight between 100 and 140 kDa. The optimal activity of the purified enzyme occurred at a pH of 6.0 and at a temperature of 50 °C. The stability between different pH and temperature ranges along with the effect of chemical metal ions and inhibitors were also studied. Our results reveal that the purified enzyme could be used in the dairy industry such as in accelerated cheese ripening. Full article

(This article belongs to the Section Physical Chemistry, Theoretical and Computational Chemistry)

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11 pages, 1303 KiB

Open AccessArticle

MicroRNAs 10a and 10b Regulate the Expression of Human Platelet Glycoprotein Ibα for Normal Megakaryopoiesis

by Zuping Zhang^1,2, Yali Ran², Tanner S. Shaw² and Yuandong Peng^2,*

¹ School of Basic Medicine, Central South University, Changsha 410013, China

² Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA

Int. J. Mol. Sci. 2016, 17(11), 1873; https://doi.org/10.3390/ijms17111873 - 9 Nov 2016

Cited by 17 | Viewed by 4920

Abstract

MicroRNAs are a class of small non-coding RNAs that bind to the three prime untranslated region (3′-UTR) of target mRNAs. They cause a cleavage or an inhibition of the translation of target mRNAs, thus regulating gene expression. Here, we employed three prediction tools [...] Read more.

MicroRNAs are a class of small non-coding RNAs that bind to the three prime untranslated region (3′-UTR) of target mRNAs. They cause a cleavage or an inhibition of the translation of target mRNAs, thus regulating gene expression. Here, we employed three prediction tools to search for potential miRNA target sites in the 3′-UTR of the human platelet glycoprotein (GP) 1BA gene. A luciferase reporter assay shows that miR-10a and -10b sites are functional. When miR-10a or -10b mimics were transfected into the GP Ibβ/GP IX-expressing cells, along with a DNA construct harboring both the coding and 3′-UTR sequences of the human GP1BA gene, we found that they inhibit the transient expression of GP Ibα on the cell surface. When the miR-10a or -10b mimics were introduced into murine progenitor cells, upon megakaryocyte differentiation, we found that GP Ibα mRNA expression was markedly reduced, suggesting that a miRNA-induced mRNA degradation is at work. Thus, our study identifies GP Ibα as a novel target of miR-10a and -10b, suggesting that a drastic reduction in the levels of miR-10a and -10b in the late stage of megakaryopoiesis is required to allow the expression of human GP Ibα and the formation of the GP Ib-IX-V complex. Full article

(This article belongs to the Collection Regulation by Non-coding RNAs)

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37 pages, 8023 KiB

Open AccessReview

p53 Proteoforms and Intrinsic Disorder: An Illustration of the Protein Structure–Function Continuum Concept

by Vladimir N. Uversky^1,2

¹ Department of Molecular Medicine and USF Health Byrd Alzheimer’s Research Institute, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd. MDC07, Tampa, FL 33612, USA

² Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave., 194064 St. Petersburg, Russia

Int. J. Mol. Sci. 2016, 17(11), 1874; https://doi.org/10.3390/ijms17111874 - 10 Nov 2016

Cited by 162 | Viewed by 13046

Abstract

Although it is one of the most studied proteins, p53 continues to be an enigma. This protein has numerous biological functions, possesses intrinsically disordered regions crucial for its functionality, can form both homo-tetramers and isoform-based hetero-tetramers, and is able to interact with many [...] Read more.

Although it is one of the most studied proteins, p53 continues to be an enigma. This protein has numerous biological functions, possesses intrinsically disordered regions crucial for its functionality, can form both homo-tetramers and isoform-based hetero-tetramers, and is able to interact with many binding partners. It contains numerous posttranslational modifications, has several isoforms generated by alternative splicing, alternative promoter usage or alternative initiation of translation, and is commonly mutated in different cancers. Therefore, p53 serves as an important illustration of the protein structure–function continuum concept, where the generation of multiple proteoforms by various mechanisms defines the ability of this protein to have a multitude of structurally and functionally different states. Considering p53 in the light of a proteoform-based structure–function continuum represents a non-canonical and conceptually new contemplation of structure, regulation, and functionality of this important protein. Full article

(This article belongs to the Special Issue Emerging Non-Canonical Functions and Regulation of p53)

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20 pages, 720 KiB

Open AccessReview

Functional Components of Carob Fruit: Linking the Chemical and Biological Space

by Vlasios Goulas¹

, Evgenios Stylos^2,3, Maria V. Chatziathanasiadou², Thomas Mavromoustakos⁴

and Andreas G. Tzakos^2,*

¹ Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, Lemesos 3603, Cyprus

² Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece

³ Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece

⁴ Laboratory of Organic Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 11571 Athens, Greece

Int. J. Mol. Sci. 2016, 17(11), 1875; https://doi.org/10.3390/ijms17111875 - 10 Nov 2016

Cited by 137 | Viewed by 14920

Abstract

The contribution of natural products to the drug-discovery pipeline has been remarkable since they have served as a rich source for drug development and discovery. Natural products have adapted, during the course of evolution, optimum chemical scaffolds against a wide variety of diseases, [...] Read more.

The contribution of natural products to the drug-discovery pipeline has been remarkable since they have served as a rich source for drug development and discovery. Natural products have adapted, during the course of evolution, optimum chemical scaffolds against a wide variety of diseases, including cancer and diabetes. Advances in high-throughput screening assays, assisted by the continuous development on the instrumentation’s capabilities and omics, have resulted in charting a large chemical and biological space of drug-like compounds, originating from natural sources. Herein, we attempt to integrate the information on the chemical composition and the associated biological impact of carob fruit in regards to human health. The beneficial and health-promoting effects of carob along with the clinical trials and the drug formulations derived from carob’s natural components are presented in this review. Full article

(This article belongs to the Special Issue The Mechanism of Action of Food Components in Disease Prevention)

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7 pages, 453 KiB

Open AccessArticle

Potential Role of Methylation Marker in Glioma Supporting Clinical Decisions

by Krzysztof Roszkowski¹

, Jacek Furtak², Bogdan Zurawski³, Tadeusz Szylberg⁴ and Marzena A. Lewandowska^5,6,*

¹ Department of Oncology, Radiotherapy and Ginecologic Oncology, Faculty of Health Sciences, Nicolaus Copernicus University, Collegium Medicum, 85-796 Bydgoszcz, Poland

² Department of Neurosurgery, Military Clinical Hospital, 85-681 Bydgoszcz, Poland

³ Outpatient Chemiotherapy The F. Lukaszczyk Oncology Center, 85-796 Bydgoszcz, Poland

⁴ Department of Pathomorphology, Military Clinical Hospital, 85-681 Bydgoszcz, Poland

⁵ Molecular Oncology and Genetics Department, The F. Lukaszczyk Oncology Center, 85-796 Bydgoszcz, Poland

⁶ Department of Thoracic Surgery and Tumors, Faculty of Medicine, Nicolaus Copernicus University, Collegium Medicum, 85-796 Bydgoszcz, Poland

Int. J. Mol. Sci. 2016, 17(11), 1876; https://doi.org/10.3390/ijms17111876 - 10 Nov 2016

Cited by 11 | Viewed by 5061

Abstract

The IDH1/2 gene mutations, ATRX loss/mutation, 1p/19q status, and MGMT promoter methylation are increasingly used as prognostic or predictive biomarkers of gliomas. However, the effect of their combination on radiation therapy outcome is discussable. Previously, we demonstrated that the IDH1 c.G395A; p.R132H [...] Read more.

The IDH1/2 gene mutations, ATRX loss/mutation, 1p/19q status, and MGMT promoter methylation are increasingly used as prognostic or predictive biomarkers of gliomas. However, the effect of their combination on radiation therapy outcome is discussable. Previously, we demonstrated that the IDH1 c.G395A; p.R132H mutation was associated with longer survival in grade II astrocytoma and GBM (Glioblastoma). Here we analyzed the MGMT promoter methylation status in patients with a known mutation status in codon 132 of IDH1, followed by clinical and genetic data analysis based on the two statuses. After a subtotal tumor resection, the patients were treated using IMRT (Intensity-Modulated Radiation Therapy) with 6 MeV photons. The total dose was: 54 Gy for astrocytoma II, 60 Gy for astrocytoma III, 60 Gy for glioblastoma, 2 Gy per day, with 24 h intervals, five days per week. The patients with MGMT promoter methylation and IDH1 somatic mutation (OS = 40 months) had a better prognosis than those with MGMT methylation alone (OS = 18 months). In patients with astrocytoma anaplasticum (n = 7) with the IDH1 p.R132H mutation and hypermethylated MGMT, the prognosis was particularly favorable (median OS = 47 months). In patients with astrocytoma II meeting the above criteria, the prognosis was also better than in those not meeting those criteria. The IDH1 mutation appears more relevant for the prognosis than MGMT methylation. The IDH1 p.R132H mutation combined with MGMT hypermethylation seems to be the most advantageous for treatment success. Patients not meeting those criteria may require more aggressive treatments. Full article

(This article belongs to the Section Biochemistry)

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21 pages, 489 KiB

Open AccessReview

Dietary Strategies Implicated in the Prevention and Treatment of Metabolic Syndrome

by Rocio De la Iglesia^1,*, Viviana Loria-Kohen¹, Maria Angeles Zulet^2,3

, Jose Alfredo Martinez^2,3

, Guillermo Reglero¹ and Ana Ramirez de Molina^1,*

¹ GENYAL Platform on Nutrition and Health, IMDEA Food Institute, CEI UAM + CSIC, 28049 Madrid, Spain

² Department of Nutrition, Food Science and Physiology, University of Navarra, 31008 Pamplona, Spain

³ CIBER Fisiopatología de la Obesidad y la Nutrición (CIBERobn), 28029 Madrid, Spain

Int. J. Mol. Sci. 2016, 17(11), 1877; https://doi.org/10.3390/ijms17111877 - 10 Nov 2016

Cited by 144 | Viewed by 20181

Abstract

Metabolic syndrome (MetS) is established as the combination of central obesity and different metabolic disturbances, such as insulin resistance, hypertension and dyslipidemia. This cluster of factors affects approximately 10%–50% of adults worldwide and the prevalence has been increasing in epidemic proportions over the [...] Read more.

Metabolic syndrome (MetS) is established as the combination of central obesity and different metabolic disturbances, such as insulin resistance, hypertension and dyslipidemia. This cluster of factors affects approximately 10%–50% of adults worldwide and the prevalence has been increasing in epidemic proportions over the last years. Thus, dietary strategies to treat this heterogenic disease are under continuous study. In this sense, diets based on negative-energy-balance, the Mediterranean dietary pattern, n-3 fatty acids, total antioxidant capacity and meal frequency have been suggested as effective approaches to treat MetS. Furthermore, the type and percentage of carbohydrates, the glycemic index or glycemic load, and dietary fiber content are some of the most relevant aspects related to insulin resistance and impaired glucose tolerance, which are important co-morbidities of MetS. Finally, new studies focused on the molecular action of specific nutritional bioactive compounds with positive effects on the MetS are currently an objective of scientific research worldwide. The present review summarizes some of the most relevant dietary approaches and bioactive compounds employed in the treatment of the MetS to date. Full article

(This article belongs to the Special Issue Advances in Nutritional Epidemiology)

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11 pages, 1252 KiB

Open AccessArticle

Increased Expression Profile and Functionality of TLR6 in Peripheral Blood Mononuclear Cells and Hepatocytes of Morbidly Obese Patients with Non-Alcoholic Fatty Liver Disease

by María Teresa Arias-Loste^1,2,*, Paula Iruzubieta^1,2, Ángela Puente^1,2, David Ramos², Carolina Santa Cruz³, Ángel Estébanez², Susana Llerena^1,2, Carmen Alonso-Martín^1,2, David San Segundo^3,4, Lorena Álvarez³, Antonio López Useros⁵, Emilio Fábrega^1,2, Marcos López-Hoyos^3,4,†

and Javier Crespo^1,2,†

¹ Gastroenterology and Hepatology Department, Marqués de Valdecilla University Hospital, 39008 Santander, Spain

² Infection, Immunity and Digestive Pathology Group, Research Institute Marqués de Valdecilla (IDIVAL), 390008 Santander, Spain

³ Transplant and Autoimmunity Group, Research Institute Marqués de Valdecilla (IDIVAL), 39008 Santander, Spain

⁴ Immunology Department, Marqués de Valdecilla University Hospital, 39008 Santander, Spain

⁵ General Surgery Department, Marqués de Valdecilla University Hospital, 39008 Santander, Spain

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1878; https://doi.org/10.3390/ijms17111878 - 10 Nov 2016

Cited by 29 | Viewed by 6065

Abstract

Current evidence suggests that gut dysbiosis drives obesity and non-alcoholic fatty liver disease (NAFLD) pathogenesis. Toll-like receptor 2 (TLR2) and TLR6 specifically recognize components of Gram-positive bacteria. Despite the potential implications of TLR2 in NAFLD pathogenesis, the role of TLR6 has not been [...] Read more.

Current evidence suggests that gut dysbiosis drives obesity and non-alcoholic fatty liver disease (NAFLD) pathogenesis. Toll-like receptor 2 (TLR2) and TLR6 specifically recognize components of Gram-positive bacteria. Despite the potential implications of TLR2 in NAFLD pathogenesis, the role of TLR6 has not been addressed. Our aim is to study a potential role of TLR6 in obesity-related NAFLD. Forty morbidly obese patients undergoing bariatric surgery were prospectively studied. Cell surface expression of TLR2 and TLR6 was assessed on peripheral blood mononuclear cells (PBMCs) by flow cytometry. Freshly isolated monocytes were cultured with specific TLR2/TLR6 agonists and intracellular production of cytokines was determined by flow-cytometry. In liver biopsies, the expression of TLR2 and TLR6 was analyzed by immunohistochemistry and cytokine gene expression using RT-qPCR. TLR6 expression in PBMCs from non-alcoholic steatohepatitis (NASH) patients was significantly higher when compared to those from simple steatosis. The production of pro-inflammatory cytokines in response to TLR2/TLR6 stimulation was also significantly higher in patients with lobular inflammation. Hepatocyte expression of TLR6 but not that of TLR2 was increased in NAFLD patients compared to normal liver histology. Deregulated expression and activity of peripheral TLR6 in morbidly obese patients can mirror the liver inflammatory events that are well known drivers of obesity-related NASH pathogenesis. Moreover, TLR6 is also significantly overexpressed in the hepatocytes of NAFLD patients compared to their normal counterparts. Thus, deregulated TLR6 expression may potentiate TLR2-mediated liver inflammation in NAFLD pathogenesis, and also serve as a potential peripheral biomarker of obesity-related NASH. Full article

(This article belongs to the Special Issue Molecular Mechanisms of Human Liver Diseases)

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10 pages, 815 KiB

Open AccessArticle

Prostate Specific Antigen (PSA) as Predicting Marker for Clinical Outcome and Evaluation of Early Toxicity Rate after High-Dose Rate Brachytherapy (HDR-BT) in Combination with Additional External Beam Radiation Therapy (EBRT) for High Risk Prostate Cancer

by Thorsten H. Ecke^1,*

, Hui-Juan Huang-Tiel², Klaus Golka³, Silvia Selinski³, Berit Christine Geis³, Stephan Koswig⁴, Katrin Bathe⁴, Steffen Hallmann¹ and Holger Gerullis⁵

¹ Department of Urology, HELIOS Hospital, D-15526 Bad Saarow, Germany

² Department of Neurology/Emergency Unit, Vivantes Hospital Spandau, D-13585 Berlin, Germany

³ Leibniz Research Centre for Working Environment and Human Factors IfADo, D-44139 Dortmund, Germany

⁴ Department of Radio-Oncology, HELIOS Hospital, D-15525 Bad Saarow, Germany

⁵ School of Medicine and Health Sciences Carl von Ossietzky, University Oldenburg, D-26133 Oldenburg, Germany

Int. J. Mol. Sci. 2016, 17(11), 1879; https://doi.org/10.3390/ijms17111879 - 10 Nov 2016

Cited by 5 | Viewed by 5656

Abstract

High-dose-rate brachytherapy (HDR-BT) with external beam radiation therapy (EBRT) is a common treatment option for locally advanced prostate cancer (PCa). Seventy-nine male patients (median age 71 years, range 50 to 79) with high-risk PCa underwent HDR-BT following EBRT between December 2009 and January [...] Read more.

High-dose-rate brachytherapy (HDR-BT) with external beam radiation therapy (EBRT) is a common treatment option for locally advanced prostate cancer (PCa). Seventy-nine male patients (median age 71 years, range 50 to 79) with high-risk PCa underwent HDR-BT following EBRT between December 2009 and January 2016 with a median follow-up of 21 months. HDR-BT was administered in two treatment sessions (one week interval) with 9 Gy per fraction using a planning system and the Ir192 treatment unit GammaMed Plus iX. EBRT was performed with CT-based 3D-conformal treatment planning with a total dose administration of 50.4 Gy with 1.8 Gy per fraction and five fractions per week. Follow-up for all patients was organized one, three, and five years after radiation therapy to evaluate early and late toxicity side effects, metastases, local recurrence, and prostate-specific antigen (PSA) value measured in ng/mL. The evaluated data included age, PSA at time of diagnosis, PSA density, BMI (body mass index), Gleason score, D’Amico risk classification for PCa, digital rectal examination (DRE), PSA value after one/three/five year(s) follow-up (FU), time of follow-up, TNM classification, prostate volume, and early toxicity rates. Early toxicity rates were 8.86% for gastrointestinal, and 6.33% for genitourinary side effects. Of all treated patients, 84.81% had no side effects. All reported complications in early toxicity were grade 1. PSA density at time of diagnosis (p = 0.009), PSA on date of first HDR-BT (p = 0.033), and PSA on date of first follow-up after one year (p = 0.025) have statistical significance on a higher risk to get a local recurrence during follow-up. HDR-BT in combination with additional EBRT in the presented design for high-risk PCa results in high biochemical control rates with minimal side-effects. PSA is a negative predictive biomarker for local recurrence during follow-up. A longer follow-up is needed to assess long-term outcome and toxicities. Full article

(This article belongs to the Special Issue Diagnostic, Prognostic and Predictive Biomarkers in Prostate Cancer)

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11 pages, 1694 KiB

Open AccessArticle

A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses

by Zhenzhou Wan^1,2,†, Ya’nan Zhang^1,†, Zhixiang He¹, Jia Liu¹, Ke Lan¹, Yihong Hu^1,* and Chiyu Zhang^1,*

¹ Pathogen Diagnostic Center, CAS Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, China

² Medical Laboratory of Taizhou Fourth People’s Hospital, Taizhou 225300, China

^† These authors contributed equally to this study.

Int. J. Mol. Sci. 2016, 17(11), 1880; https://doi.org/10.3390/ijms17111880 - 23 Nov 2016

Cited by 70 | Viewed by 15620

Abstract

Human coronaviruses HCoV-OC43, HCoV-229E, HCoV-NL63 and HCoV-HKU1 are common respiratory viruses associated with acute respiratory infection. They have a global distribution. Rapid and accurate diagnosis of HCoV infection is important for the management and treatment of hospitalized patients with HCoV infection. Here, we [...] Read more.

Human coronaviruses HCoV-OC43, HCoV-229E, HCoV-NL63 and HCoV-HKU1 are common respiratory viruses associated with acute respiratory infection. They have a global distribution. Rapid and accurate diagnosis of HCoV infection is important for the management and treatment of hospitalized patients with HCoV infection. Here, we developed a melting curve-based multiplex RT-qPCR assay for simultaneous detection of the four HCoVs. In the assay, SYTO 9 was used to replace SYBR Green I as the fluorescent dye, and GC-modified primers were designed to improve the melting temperature (Tm) of the specific amplicon. The four HCoVs were clearly distinguished by characteristic melting peaks in melting curve analysis. The detection sensitivity of the assay was 3 × 10² copies for HCoV-OC43, and 3 × 10¹ copies for HCoV-NL63, HCoV-229E and HCoV-HKU1 per 30 μL reaction. Clinical evaluation and sequencing confirmation demonstrated that the assay was specific and reliable. The assay represents a sensitive and reliable method for diagnosis of HCoV infection in clinical samples. Full article

(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)

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15 pages, 5189 KiB

Open AccessArticle

Transcriptome Analysis Identifies Key Candidate Genes Mediating Purple Ovary Coloration in Asiatic Hybrid Lilies

by Leifeng Xu¹

, Panpan Yang^1,2, Suxia Yuan¹, Yayan Feng¹, Hua Xu¹, Yuwei Cao¹ and Jun Ming^1,*

¹ Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

² College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China

Int. J. Mol. Sci. 2016, 17(11), 1881; https://doi.org/10.3390/ijms17111881 - 20 Nov 2016

Cited by 17 | Viewed by 8729

Abstract

Lily tepals have a short lifespan. Once the tepals senesce, the ornamental value of the flower is lost. Some cultivars have attractive purple ovaries and fruits which greatly enhance the ornamental value of Asiatic hybrid lilies. However, little is known about the molecular [...] Read more.

Lily tepals have a short lifespan. Once the tepals senesce, the ornamental value of the flower is lost. Some cultivars have attractive purple ovaries and fruits which greatly enhance the ornamental value of Asiatic hybrid lilies. However, little is known about the molecular mechanisms of anthocyanin biosynthesis in Asiatic hybrid lily ovaries. To investigate the transcriptional network that governs purple ovary coloration in Asiatic hybrid lilies, we obtained transcriptome data from green ovaries (S1) and purple ovaries (S2) of Asiatic “Tiny Padhye”. Comparative transcriptome analysis revealed 4228 differentially expressed genes. Differential expression analysis revealed that ten unigenes including four CHS genes, one CHI gene, one F3H gene, one F3′H gene, one DFR gene, one UFGT gene, and one 3RT gene were significantly up-regulated in purple ovaries. One MYB gene, LhMYB12-Lat, was identified as a key transcription factor determining the distribution of anthocyanins in Asiatic hybrid lily ovaries. Further qPCR results showed unigenes related to anthocyanin biosynthesis were highly expressed in purple ovaries of three purple-ovaried Asiatic hybrid lilies at stages 2 and 3, while they showed an extremely low level of expression in ovaries of three green-ovaried Asiatic hybrid lilies during all developmental stages. In addition, shading treatment significantly decreased pigment accumulation by suppressing the expression of several unigenes related to anthocyanin biosynthesis in ovaries of Asiatic “Tiny Padhye”. Lastly, a total of 15,048 Simple Sequence Repeats (SSRs) were identified in 13,710 sequences, and primer pairs for SSRs were designed. The results could further our understanding of the molecular mechanisms of anthocyanin biosynthesis in Asiatic hybrid lily ovaries. Full article

(This article belongs to the Special Issue Anthocyanins)

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14 pages, 4476 KiB

Open AccessArticle

Dominant Suppression of β1 Integrin by Ectopic CD98-ICD Inhibits Hepatocellular Carcinoma Progression

by Bo Wu^1,†, Yang Zhou^1,†, Yu Wang^2,3,†, Xiang-Min Yang¹, Zhen-Yu Liu¹, Jiang-Hua Li¹, Fei Feng¹, Zhi-Nan Chen^1,* and Jian-Li Jiang^1,*

¹ National Translational Science Center for Molecular Medicine, Cell Engineering Research Centre and Department of Cell Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi’an 710032, China

² Department of Oncology, State Key Discipline of Cell Biology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

³ Institute of Stomatology, Chinese People’s Liberation Army General Hospital, Beijing 100853, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1882; https://doi.org/10.3390/ijms17111882 - 10 Nov 2016

Cited by 7 | Viewed by 5486

Abstract

Hepatocellular carcinoma (HCC) is currently the third most common cause of cancer-related death in the Asia-Pacific region. Our previous work showed that knockdown of CD98 significantly inhibits malignant HCC cell phenotypes in vitro and in vivo. The level of CD98 in the membrane [...] Read more.

Hepatocellular carcinoma (HCC) is currently the third most common cause of cancer-related death in the Asia-Pacific region. Our previous work showed that knockdown of CD98 significantly inhibits malignant HCC cell phenotypes in vitro and in vivo. The level of CD98 in the membrane is tightly regulated to mediate complex processes associated with cell–cell communication and intracellular signaling. In addition, the intracellular domain of CD98 (CD98-ICD) seems to be of vital importance for recycling CD98 to the membrane after it is endocytosed. The intracellular and transmembrane domains of CD98 associate with β-integrins (primarily β1 but also β3), and this association is essential for CD98 mediation of integrin-like signaling and complements dominant suppression of β1-integrin. We speculated that isolated CD98-ICD would similarly suppress β1-integrin activation and inhibit the malignant behaviors of cancer cells. In particular, the exact role of CD98-ICD has not been studied independently in HCC. In this study, we found that ectopic expression of CD98-ICD inhibited the malignant phenotypes of HCC cells, and the mechanism possibly involves β1-integrin suppression. Moreover, the expression levels of CD98, β1-integrin-A (the activated form of β1-integrin) and Ki-67 were significantly increased in HCC tissues relative to those of normal liver tissues. Therefore, our preliminary study indicates that ectopic CD98-ICD has an inhibitory role in the malignant development of HCC, and shows that CD98-ICD acts as a dominant negative mutant of CD98 that attenuates β1-integrin activation. CD98-ICD may emerge as a promising candidate for antitumor treatment. Full article

(This article belongs to the Special Issue Molecular Mechanisms of Human Liver Diseases)

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13 pages, 8493 KiB

Open AccessArticle

Functional Studies and In Silico Analyses to Evaluate Non-Coding Variants in Inherited Cardiomyopathies

by Giulia Frisso^1,2,†

, Nicola Detta^1,†, Pamela Coppola^1,2, Cristina Mazzaccara^1,2

, Maria Rosaria Pricolo², Antonio D’Onofrio³, Giuseppe Limongelli³

, Raffaele Calabrò³ and Francesco Salvatore^1,4,*

¹ CEINGE-Biotecnologie Avanzate s.c.a r.l., 80145 Napoli, Italy

² Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, 80131 Napoli, Italy

³ Dipartimento di Cardiologia, Seconda Università degli Studi di Napoli, A.O. Monaldi, Azienda dei Colli, 80131 Napoli, Italy

⁴ IRCCS-Fondazione SDN, 80143 Napoli, Italy

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1883; https://doi.org/10.3390/ijms17111883 - 10 Nov 2016

Cited by 28 | Viewed by 5442

Abstract

Point mutations are the most common cause of inherited diseases. Bioinformatics tools can help to predict the pathogenicity of mutations found during genetic screening, but they may work less well in determining the effect of point mutations in non-coding regions. In silico analysis [...] Read more.

Point mutations are the most common cause of inherited diseases. Bioinformatics tools can help to predict the pathogenicity of mutations found during genetic screening, but they may work less well in determining the effect of point mutations in non-coding regions. In silico analysis of intronic variants can reveal their impact on the splicing process, but the consequence of a given substitution is generally not predictable. The aim of this study was to functionally test five intronic variants (MYBPC3-c.506-2A>C, MYBPC3-c.906-7G>T, MYBPC3-c.2308+3G>C, SCN5A-c.393-5C>A, and ACTC1-c.617-7T>C) found in five patients affected by inherited cardiomyopathies in the attempt to verify their pathogenic role. Analysis of the MYBPC3-c.506-2A>C mutation in mRNA from the peripheral blood of one of the patients affected by hypertrophic cardiac myopathy revealed the loss of the canonical splice site and the use of an alternative splicing site, which caused the loss of the first seven nucleotides of exon 5 (MYBPC3-G169AfsX14). In the other four patients, we generated minigene constructs and transfected them in HEK-293 cells. This minigene approach showed that MYBPC3-c.2308+3G>C and SCN5A-c.393-5C>A altered pre-mRNA processing, thus resulting in the skipping of one exon. No alterations were found in either MYBPC3-c.906-7G>T or ACTC1-c.617-7T>C. In conclusion, functional in vitro analysis of the effects of potential splicing mutations can confirm or otherwise the putative pathogenicity of non-coding mutations, and thus help to guide the patient's clinical management and improve genetic counseling in affected families. Full article

(This article belongs to the Special Issue Pre-mRNA Splicing 2016)

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15 pages, 869 KiB

Open AccessReview

Role of Osteogenic Growth Peptide (OGP) and OGP(10–14) in Bone Regeneration: A Review

by Suzane C. Pigossi^1,2

, Marcell C. Medeiros¹

, Sybele Saska³, Joni A. Cirelli¹

and Raquel M. Scarel-Caminaga^1,2,*

¹ Department of Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP–São Paulo State University, Humaita St, 1680, CEP 14801-903 Araraquara, São Paulo, Brazil

² Department of Morphology, School of Dentistry, UNESP– São Paulo State University, Humaita St, 1680, CEP 14801-903 Araraquara, São Paulo, Brazil

³ Department of General and Inorganic Chemistry, Institute of Chemistry, UNESP–São Paulo State University, Professor Francisco Degni St, 55, CEP 14800-900 Araraquara, São Paulo, Brazil

Int. J. Mol. Sci. 2016, 17(11), 1885; https://doi.org/10.3390/ijms17111885 - 22 Nov 2016

Cited by 61 | Viewed by 10275

Abstract

Bone regeneration is a process that involves several molecular mediators, such as growth factors, which directly affect the proliferation, migration and differentiation of bone-related cells. The osteogenic growth peptide (OGP) and its C-terminal pentapeptide OGP(10–14) have been shown to stimulate the proliferation, differentiation, [...] Read more.

Bone regeneration is a process that involves several molecular mediators, such as growth factors, which directly affect the proliferation, migration and differentiation of bone-related cells. The osteogenic growth peptide (OGP) and its C-terminal pentapeptide OGP(10–14) have been shown to stimulate the proliferation, differentiation, alkaline phosphatase activity and matrix mineralization of osteoblastic lineage cells. However, the exact molecular mechanisms that promote osteoblastic proliferation and differentiation are not completely understood. This review presents the main chemical characteristics of OGP and/or OGP(10–14), and also discusses the potential molecular pathways induced by these growth factors to promote proliferation and differentiation of osteoblasts. Furthermore, since these peptides have been extensively investigated for bone tissue engineering, the clinical applications of these peptides for bone regeneration are discussed. Full article

(This article belongs to the Special Issue Advances in Bone and Cartilage Research)

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12 pages, 424 KiB

Open AccessArticle

Altered Episodic Memory in Introverted Young Adults Carrying the BDNF_Met Allele

by Andreanne Bombardier¹, Maude Beauchemin², Nadia Gosselin^2,3, Judes Poirier⁴ and Louis De Beaumont^1,3,*

¹ Department of Psychology, University of Quebec at Trois-Rivieres, 3351 Boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada

² Department of Psychology, University of Montreal, 2900 Boulevard Edouard-Montpetit, Montréal, QC H3T 1J4, Canada

³ Montreal Sacred Heart Hospital Research Centre, Montreal, QC H4J 1C5, Canada

⁴ Douglas Mental Health University Institute, 6875 Boulevard LaSalle, Verdun, QC H4H 1R3, Canada

Int. J. Mol. Sci. 2016, 17(11), 1886; https://doi.org/10.3390/ijms17111886 - 12 Nov 2016

Cited by 3 | Viewed by 5014

Abstract

While most studies have been interested in the distinct, predisposing roles of the common BDNF Val66Met variant and extraversion personality traits on episodic memory, very few studies have looked at the synergistic effects of genetic and personality factors to account for cognitive variance. [...] Read more.

While most studies have been interested in the distinct, predisposing roles of the common BDNF Val66Met variant and extraversion personality traits on episodic memory, very few studies have looked at the synergistic effects of genetic and personality factors to account for cognitive variance. This is surprising considering recent reports challenging the long-held belief that the BDNF_Met variant negatively impacts cognitive function. A total of 75 young healthy adults (26 of them carried at least one copy of the BDNF_Met allele) took part in this study consisting of genetic profiling from saliva, personality assessment using the Revised NEO Personality Inventory (NEO PI-R) and a short battery of neuropsychological tests. An ANOVA revealed that BDNF_Met carriers were significantly less extraverted than BDNF_Val carriers (F_1,73 = 9.54; p < 0.01; η_p² = 0.126). Moreover, extraversion was found to significantly moderate the relationship between the BDNF genotype and episodic memory performance (p = 0.03). Subsequent correlational analyses yielded a strong and significant correlation (r = 0.542; p < 0.005) between introversion and delayed episodic memory specific to BDNF_Met individuals. The present study suggests that introversion and the BDNF_Met variant synergistically interact to reduce episodic memory performance in healthy, young adults. These findings reaffirm that a more accurate explanation of cognitive variance can be achieved by looking at the synergistic effects of genotype and phenotype factors. Full article

(This article belongs to the Special Issue Neurotrophic Factors—Historical Perspective and New Directions)

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19 pages, 2026 KiB

Open AccessArticle

Generation and Characterisation of a Reference Transcriptome for Lentil (Lens culinaris Medik.)

by Shimna Sudheesh¹, Preeti Verma¹, John W. Forster^1,2, Noel O. I. Cogan^1,2 and Sukhjiwan Kaur^1,*

¹ Biosciences Research, Agriculture Victoria, AgriBio, 5 Ring Road, La Trobe University, Bundoora, VIC 3083, Australia

² School of Applied Systems Biology, La Trobe University, Bundoora, VIC 3086, Australia

Int. J. Mol. Sci. 2016, 17(11), 1887; https://doi.org/10.3390/ijms17111887 - 12 Nov 2016

Cited by 52 | Viewed by 7210

Abstract

RNA-Seq using second-generation sequencing technologies permits generation of a reference unigene set for a given species, in the absence of a well-annotated genome sequence, supporting functional genomics studies, gene characterisation and detailed expression analysis for specific morphophysiological or environmental stress response traits. A [...] Read more.

RNA-Seq using second-generation sequencing technologies permits generation of a reference unigene set for a given species, in the absence of a well-annotated genome sequence, supporting functional genomics studies, gene characterisation and detailed expression analysis for specific morphophysiological or environmental stress response traits. A reference unigene set for lentil has been developed, consisting of 58,986 contigs and scaffolds with an N50 length of 1719 bp. Comparison to gene complements from related species, reference protein databases, previously published lentil transcriptomes and a draft genome sequence validated the current dataset in terms of degree of completeness and utility. A large proportion (98%) of unigenes were expressed in more than one tissue, at varying levels. Candidate genes associated with mechanisms of tolerance to both boron toxicity and time of flowering were identified, which can eventually be used for the development of gene-based markers. This study has provided a comprehensive, assembled and annotated reference gene set for lentil that can be used for multiple applications, permitting identification of genes for pathway-specific expression analysis, genetic modification approaches, development of resources for genotypic analysis, and assistance in the annotation of a future lentil genome sequence. Full article

(This article belongs to the Special Issue Pulses)

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13 pages, 1793 KiB

Open AccessArticle

Procyanidin A2 Modulates IL-4-Induced CCL26 Production in Human Alveolar Epithelial Cells

by Sara L. Coleman^1,2,*, Marlena C. Kruger²

, Gregory M. Sawyer¹

and Roger D. Hurst¹

¹ Food and Wellness Group, The New Zealand Institute for Plant & Food Research Ltd., Palmerston North 4474, New Zealand

² School of Food and Nutrition, Massey Institute of Food Science and Technology, Massey University, Palmerston North 4442, New Zealand

Int. J. Mol. Sci. 2016, 17(11), 1888; https://doi.org/10.3390/ijms17111888 - 12 Nov 2016

Cited by 20 | Viewed by 7935

Abstract

Allergic asthma is an inflammatory lung disease that is partly sustained by the chemokine eotaxin-3 (CCL26), which extends eosinophil migration into tissues long after allergen exposure. Modulation of CCL26 could represent a means to mitigate airway inflammation. Here we evaluated procyanidin A2 as [...] Read more.

Allergic asthma is an inflammatory lung disease that is partly sustained by the chemokine eotaxin-3 (CCL26), which extends eosinophil migration into tissues long after allergen exposure. Modulation of CCL26 could represent a means to mitigate airway inflammation. Here we evaluated procyanidin A2 as a means of modulating CCL26 production and investigated interactions with the known inflammation modulator, Interferon γ (IFNγ). We used the human lung epithelial cell line A549 and optimized the conditions for inducing CCL26. Cells were exposed to a range of procyanidin A2 or IFNγ concentrations for varied lengths of time prior to an inflammatory insult of interleukin-4 (IL-4) for 24 h. An enzyme-linked immunosorbent assay was used to measure CCL26 production. Exposing cells to 5 μM procyanidin A2 (prior to IL-4) reduced CCL26 production by 35% compared with control. Greatest inhibition by procyanidin A2 was seen with a 2 h exposure prior to IL-4, whereas IFNγ inhibition was greatest at 24 h. Concomitant incubation of procyanidin A2 and IFNγ did not extend the inhibitory efficacy of procyanidin A2. These data provide evidence that procyanidin A2 can modulate IL-4-induced CCL26 production by A549 lung epithelial cells and that it does so in a manner that is different from IFNγ. Full article

(This article belongs to the Special Issue Natural Anti-Inflammatory Agents)

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13 pages, 2242 KiB

Open AccessReview

Unsaturated Lipids Change in Olive Tree Drupe and Seed during Fruit Development and in Response to Cold-Stress and Acclimation

by Simone D’Angeli

and Maria Maddalena Altamura^*

Dipartimento di Biologia Ambientale, Sapienza University of Rome, 00185 Roma, Italy

Int. J. Mol. Sci. 2016, 17(11), 1889; https://doi.org/10.3390/ijms17111889 - 12 Nov 2016

Cited by 35 | Viewed by 6982

Abstract

The olive tree is a plant of economic value for the oil of its drupe. It is a cultigen complex composed of genotypes with differences in cold-hardiness. About 90% of the oil is stored in oil bodies (OBs) in the drupe during the [...] Read more.

The olive tree is a plant of economic value for the oil of its drupe. It is a cultigen complex composed of genotypes with differences in cold-hardiness. About 90% of the oil is stored in oil bodies (OBs) in the drupe during the oleogenic phase. Phenols and lipids contribute to oil quality, but the unsaturated fatty acid (FA) fraction is emerging as the most important for quality, because of the very high content in oleic acid, the presence of ω6-linoleic acid and ω3-linolenic acid, and the very low saturated FA content. Another 10% of oil is produced by the seed. Differences in unsaturated FA-enriched lipids exist among seed coat, endosperm, and embryo. Olive oil quality is also affected by the environmental conditions during fruit growth and genotype peculiarities. Production of linoleic and α-linolenic acids, fruit growth, fruit and leaf responses to low temperatures, including cuticle formation, and cold-acclimation are related processes. The levels of unsaturated FAs are changed by FA-desaturase (FAD) activities, involving the functioning of chloroplasts and endoplasmic reticulum. Cold induces lipid changes during drupe and seed development, affecting FADs, but its effect is related to the genotype capability to acclimate to the cold. Full article

(This article belongs to the Special Issue Lipidomics and Glycomics: New Advances in Food Science and Nutrition)

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10 pages, 522 KiB

Open AccessReview

Severe Cutaneous Adverse Reactions: The Pharmacogenomics from Research to Clinical Implementation

by Shih-Chi Su^1,2, Shuen-Iu Hung³

, Wen-Lang Fan¹, Ro-Lan Dao³ and Wen-Hung Chung^1,3,4,*

¹ Whole-Genome Research Core Laboratory of Human Diseases, Chang Gung Memorial Hospital, Keelung 204, Taiwan

² Department of Dermatology, Drug Hypersensitivity Clinical and Research Center, Chang Gung Memorial Hospital, Taipei, Linkou and Keelung 105, Taiwan

³ Institute of Pharmacology, School of Medicine, Infection and Immunity Research Center, National Yang-Ming University, Taipei 112, Taiwan

⁴ College of Medicine, Chang Gung University, Taoyuan 333, Taiwan

Int. J. Mol. Sci. 2016, 17(11), 1890; https://doi.org/10.3390/ijms17111890 - 15 Nov 2016

Cited by 39 | Viewed by 8379

Abstract

Severe cutaneous adverse reactions (SCARs), previously thought to be idiosyncratic or unpredictable, are a deadly form of adverse drug reactions with skin manifestations. Current pharmacogenomic studies of SCARs have made important strides, as the prevention of SCARs, to some extent, appears attainable with [...] Read more.

Severe cutaneous adverse reactions (SCARs), previously thought to be idiosyncratic or unpredictable, are a deadly form of adverse drug reactions with skin manifestations. Current pharmacogenomic studies of SCARs have made important strides, as the prevention of SCARs, to some extent, appears attainable with the identification of genetic variants for genes encoding drug-metabolizing enzymes and human leukocyte antigens (HLAs). Despite the improvement of incidence, a treatment guideline for this devastating condition is still unavailable, highlighting the inadequacy of contemporary accepted therapeutic interventions. As such, prompt withdrawal of causative drugs is believed to be a priority of patient management. In this review, we discuss recent cutting-edge findings concerning the discovery of biomarkers for SCARs and their clinical utilities in the better prediction and early diagnosis of this disease. The knowledge compiled herein provides clues for future investigations on deciphering additional genetic markers for SCARs and the design of clinical trials for the prospective identification of subjects at genetic risk for this condition, ultimately personalizing the medicine. Full article

(This article belongs to the Special Issue Precision Medicine—From Bench to Bedside)

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14 pages, 666 KiB

Open AccessReview

Insect Gallers and Their Plant Hosts: From Omics Data to Systems Biology

by Caryn N. Oates¹

, Katherine J. Denby²

, Alexander A. Myburg¹, Bernard Slippers¹ and Sanushka Naidoo^1,*

¹ Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), Genomics Research Institute (GRI), University of Pretoria, Private Bag x20, Pretoria 0028, South Africa

² Department of Biology, University of York, Wentworth Way, York YO10 5DD, UK

Int. J. Mol. Sci. 2016, 17(11), 1891; https://doi.org/10.3390/ijms17111891 - 18 Nov 2016

Cited by 35 | Viewed by 9303

Abstract

Gall-inducing insects are capable of exerting a high level of control over their hosts’ cellular machinery to the extent that the plant’s development, metabolism, chemistry, and physiology are all altered in favour of the insect. Many gallers are devastating pests in global agriculture [...] Read more.

Gall-inducing insects are capable of exerting a high level of control over their hosts’ cellular machinery to the extent that the plant’s development, metabolism, chemistry, and physiology are all altered in favour of the insect. Many gallers are devastating pests in global agriculture and the limited understanding of their relationship with their hosts prevents the development of robust management strategies. Omics technologies are proving to be important tools in elucidating the mechanisms involved in the interaction as they facilitate analysis of plant hosts and insect effectors for which little or no prior knowledge exists. In this review, we examine the mechanisms behind insect gall development using evidence from omics-level approaches. The secretion of effector proteins and induced phytohormonal imbalances are highlighted as likely mechanisms involved in gall development. However, understanding how these components function within the system is far from complete and a number of questions need to be answered before this information can be used in the development of strategies to engineer or breed plants with enhanced resistance. Full article

(This article belongs to the Special Issue Plant-Insect Interactions)

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16 pages, 1664 KiB

Open AccessReview

CPP-Assisted Intracellular Drug Delivery, What Is Next?

by Junxiao Ye^1,2,†, Ergang Liu^1,2,†, Zhili Yu², Xing Pei², Sunhui Chen², Pengwei Zhang^1,2, Meong-Cheol Shin³, Junbo Gong¹, Huining He^2,* and Victor C. Yang^2,4,*

¹ Collaborative Innovation Center of Chemical Science and Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

² Tianjin Key Laboratory on Technologies Enabling, Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China

³ College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinjudaero, Jinju, Gyeongnam 660-751, Korea

⁴ Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109-1065, USA

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1892; https://doi.org/10.3390/ijms17111892 - 14 Nov 2016

Cited by 83 | Viewed by 10403

Abstract

For the past 20 years, we have witnessed an unprecedented and, indeed, rather miraculous event of how cell-penetrating peptides (CPPs), the naturally originated penetrating enhancers, help overcome the membrane barrier that has hindered the access of bio-macromolecular compounds such as genes and proteins [...] Read more.

For the past 20 years, we have witnessed an unprecedented and, indeed, rather miraculous event of how cell-penetrating peptides (CPPs), the naturally originated penetrating enhancers, help overcome the membrane barrier that has hindered the access of bio-macromolecular compounds such as genes and proteins into cells, thereby denying their clinical potential to become potent anti-cancer drugs. By taking the advantage of the unique cell-translocation property of these short peptides, various payloads of proteins, nucleic acids, or even nanoparticle-based carriers were delivered into all cell types with unparalleled efficiency. However, non-specific CPP-mediated cell penetration into normal tissues can lead to widespread organ distribution of the payloads, thereby reducing the therapeutic efficacy of the drug and at the same time increasing the drug-induced toxic effects. In view of these challenges, we present herein a review of the new designs of CPP-linked vehicles and strategies to achieve highly effective yet less toxic chemotherapy in combating tumor oncology. Full article

(This article belongs to the Special Issue Cell-Penetrating Peptides 2016)

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17 pages, 1641 KiB

Open AccessArticle

The Thermodynamic and Kinetic Properties of 2-Hydroxypyridine/2-Pyridone Tautomerization: A Theoretical and Computational Revisit

by Safiyah A. Hejazi¹, Osman I. Osman^1,2

, Abdulrahman O. Alyoubi¹, Saadullah G. Aziz¹ and Rifaat H. Hilal^1,3,*

¹ Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia

² Chemistry Department, Faculty of Science, University of Khartoum, P.O. Box 321, Khartoum 11111, Sudan

³ Chemistry Department, Faculty of Science, Cairo University, Cairo 11258, Egypt

Int. J. Mol. Sci. 2016, 17(11), 1893; https://doi.org/10.3390/ijms17111893 - 14 Nov 2016

Cited by 32 | Viewed by 7041

Abstract

The gas-phase thermal tautomerization reaction between 2-hydroxypyridine (2-HPY) and 2-pyridone (2-PY) was investigated by applying 6-311++G** and aug-cc-pvdz basis sets incorporated into some density functional theory (DFT) and coupled cluster with singles and doubles (CCSD) methods. The geometrical structures, dipole moments, HOMO-LUMO energy [...] Read more.

The gas-phase thermal tautomerization reaction between 2-hydroxypyridine (2-HPY) and 2-pyridone (2-PY) was investigated by applying 6-311++G** and aug-cc-pvdz basis sets incorporated into some density functional theory (DFT) and coupled cluster with singles and doubles (CCSD) methods. The geometrical structures, dipole moments, HOMO-LUMO energy gaps, total hyperpolarizability, kinetics and thermodynamics functions were monitored against the effects of the corrections imposed on these functionals. The small experimental energy difference between the two tautomers of 3.23 kJ/mol; was a real test of the accuracy of the applied levels of theory. M062X and CCSD methods predicted the preference of 2-HPY over 2-PY by 5–9 kJ/mol; while B3LYP functional favoured 2-PY by 1–3 kJ/mol. The CAM-B3LYP and ωB97XD functionals yielded mixed results depending on the basis set used. The source of preference of 2-HPY is the minimal steric hindrance and electrostatic repulsion that subdued the huge hyperconjugation in 2-PY. A 1,3-proton shift intramolecular gas-phase tautomerization yielded a high average activation of 137.152 kJ/mol; while the intermolecular mixed dimer interconversion gave an average barrier height of 30.844 kJ/mol. These findings are boosted by a natural bond orbital (NBO) technique. The low total hyperpolarizabilities of both tautomers mark out their poor nonlinear optical (NLO) behaviour. The enhancement of the total hyperpolarizability of 2-HPY over that of 2-PY is interpreted by the bond length alternation. Full article

(This article belongs to the Special Issue Chemical Bond and Bonding 2016)

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13 pages, 1457 KiB

Open AccessReview

Optical Coherence Tomography and Magnetic Resonance Imaging in Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorder

by Praveena Manogaran¹, James V. M. Hanson^1,2, Elisabeth D. Olbert¹, Christine Egger¹, Carla Wicki¹, Christina Gerth-Kahlert², Klara Landau² and Sven Schippling^1,*

¹ Neuroimmunology and Multiple Sclerosis Research, Department of Neurology, University Hospital Zürich and University of Zurich, Frauenklinikstrasse 26, 8091 Zurich, Switzerland

² Department of Ophthalmology, University Hospital Zurich and University of Zurich, Frauenklinikstrasse 24, 8091 Zurich, Switzerland

Int. J. Mol. Sci. 2016, 17(11), 1894; https://doi.org/10.3390/ijms17111894 - 15 Nov 2016

Cited by 30 | Viewed by 8760

Abstract

Irreversible disability in multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD) is largely attributed to neuronal and axonal degeneration, which, along with inflammation, is one of the major pathological hallmarks of these diseases. Optical coherence tomography (OCT) is a non-invasive imaging tool [...] Read more.

Irreversible disability in multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD) is largely attributed to neuronal and axonal degeneration, which, along with inflammation, is one of the major pathological hallmarks of these diseases. Optical coherence tomography (OCT) is a non-invasive imaging tool that has been used in MS, NMOSD, and other diseases to quantify damage to the retina, including the ganglion cells and their axons. The fact that these are the only unmyelinated axons within the central nervous system (CNS) renders the afferent visual pathway an ideal model for studying axonal and neuronal degeneration in neurodegenerative diseases. Structural magnetic resonance imaging (MRI) can be used to obtain anatomical information about the CNS and to quantify evolving pathology in MS and NMOSD, both globally and in specific regions of the visual pathway including the optic nerve, optic radiations and visual cortex. Therefore, correlations between brain or optic nerve abnormalities on MRI, and retinal pathology using OCT, may shed light on how damage to one part of the CNS can affect others. In addition, these imaging techniques can help identify important differences between MS and NMOSD such as disease-specific damage to the visual pathway, trans-synaptic degeneration, or pathological changes independent of the underlying disease process. This review focuses on the current knowledge of the role of the visual pathway using OCT and MRI in patients with MS and NMOSD. Emphasis is placed on studies that employ both MRI and OCT to investigate damage to the visual system in these diseases. Full article

(This article belongs to the Special Issue Advances in Multiple Sclerosis 2016)

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18 pages, 515 KiB

Open AccessReview

Taking Advantage of Nature’s Gift: Can Endogenous Neural Stem Cells Improve Myelin Regeneration?

by Rainer Akkermann¹

, Janusz Joachim Jadasz¹, Kasum Azim² and Patrick Küry^1,*

¹ Department of Neurology, Medical Faculty, Heinrich-Heine-University, 40225 Düsseldorf, Germany

² Focus Translational Neuroscience, Institute of Physiological Chemistry, University of Mainz, 55122 Mainz, Germany

Int. J. Mol. Sci. 2016, 17(11), 1895; https://doi.org/10.3390/ijms17111895 - 14 Nov 2016

Cited by 9 | Viewed by 6842

Abstract

Irreversible functional deficits in multiple sclerosis (MS) are directly correlated to axonal damage and loss. Neurodegeneration results from immune-mediated destruction of myelin sheaths and subsequent axonal demyelination. Importantly, oligodendrocytes, the myelinating glial cells of the central nervous system, can be replaced to some [...] Read more.

Irreversible functional deficits in multiple sclerosis (MS) are directly correlated to axonal damage and loss. Neurodegeneration results from immune-mediated destruction of myelin sheaths and subsequent axonal demyelination. Importantly, oligodendrocytes, the myelinating glial cells of the central nervous system, can be replaced to some extent to generate new myelin sheaths. This endogenous regeneration capacity has so far mainly been attributed to the activation and recruitment of resident oligodendroglial precursor cells. As this self-repair process is limited and increasingly fails while MS progresses, much interest has evolved regarding the development of remyelination-promoting strategies and the presence of alternative cell types, which can also contribute to the restoration of myelin sheaths. The adult brain comprises at least two neurogenic niches harboring life-long adult neural stem cells (NSCs). An increasing number of investigations are beginning to shed light on these cells under pathological conditions and revealed a significant potential of NSCs to contribute to myelin repair activities. In this review, these emerging investigations are discussed with respect to the importance of stimulating endogenous repair mechanisms from germinal sources. Moreover, we present key findings of NSC-derived oligodendroglial progeny, including a comprehensive overview of factors and mechanisms involved in this process. Full article

(This article belongs to the Special Issue Advances in Multiple Sclerosis 2016)

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13 pages, 533 KiB

Open AccessArticle

Effect of Post-Translational Amidation on Islet Amyloid Polypeptide Conformational Ensemble: Implications for Its Aggregation Early Steps

by Linh Tran

and Tâp Ha-Duong^*

BioCIS, Université Paris-Sud, CNRS, Université Paris-Saclay, 92290 Châtenay-Malabry, France

Int. J. Mol. Sci. 2016, 17(11), 1896; https://doi.org/10.3390/ijms17111896 - 14 Nov 2016

Cited by 10 | Viewed by 4387

Abstract

The human islet amyloid polypeptide (hIAPP) is an intrinsically disordered protein that can self-assemble into fibrillar aggregates that play a key role in the pathogenesis of the type II diabetes mellitus. hIAPP can transiently adopt

α

-helix and

β

-strand conformations that could [...] Read more.

The human islet amyloid polypeptide (hIAPP) is an intrinsically disordered protein that can self-assemble into fibrillar aggregates that play a key role in the pathogenesis of the type II diabetes mellitus. hIAPP can transiently adopt

α

-helix and

β

-strand conformations that could be important intermediate species on the fibrillization pathway. However, experimental studies of the monomeric peptide conformations are limited due to its high aggregation propensity, and the early steps of the hIAPP association are not clearly characterized. In particular, the question of whether the aggregation-prone conformation is

α

-helical or

β

-strand-rich is still debated. In this study, combining extensive all-atom molecular dynamics (MD) and replica exchange molecular dynamics (REMD) simulations in explicit water, we shed some light on the differences between the amidated and non-amidated hIAPP conformational ensembles. Our study shows that, when compared to the amidated monomer, the non-amidation of hIAPP induces a significantly lower propensity to form

β

-strands, especially aggregation-prone

β

-hairpins. Since the fibrillization of the non-amidated hIAPP is significantly slower than that of the amidated peptide, this indicates that the early steps of the peptide oligomerization involve the association of

β

-hairpins or

β

-strands structures. Full article

(This article belongs to the Section Biochemistry)

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14 pages, 2884 KiB

Open AccessArticle

Fabrication and Optimization of Stable, Optically Transparent, and Reusable pH-Responsive Silk Membranes

by Andreas Toytziaridis and Cedric Dicko^*

Pure and Applied Biochemistry, Chemical Center, Lund University, 22241 Lund, Sweden

Int. J. Mol. Sci. 2016, 17(11), 1897; https://doi.org/10.3390/ijms17111897 - 15 Nov 2016

Viewed by 5258

Abstract

The fabrication of silk-based membranes that are stable, optically transparent and reusable is yet to be achieved. To address this bottleneck we have developed a method to produce transparent chromogenic silk patches that are optically responsive to pH. The patches were produced by [...] Read more.

The fabrication of silk-based membranes that are stable, optically transparent and reusable is yet to be achieved. To address this bottleneck we have developed a method to produce transparent chromogenic silk patches that are optically responsive to pH. The patches were produced by blending regenerated silk fibroin (RSF), Laponite^® RD (nano clay) and the organic dyes neutral red and Thionine acetate. The Laponite^® RD played a central role in the patch mechanical integrity and prevention of dye leaching. The process was optimized using a factorial design to maximize the patch response to pH by UV absorbance and fluorescence emission. New patches of the optimized protocol, made from solutions containing 125 μM neutral red or 250 μM of Thionine and 15 mg/mL silk, were further tested for operational stability over several cycles of pH altering. Stability, performance, and reusability were achieved over the tested cycles. The approach could be extended to other reporting molecules or enzymes able to bind to Laponite^®. Full article

(This article belongs to the Special Issue Silk-Based Materials: From Production to Characterization)

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13 pages, 255 KiB

Open AccessArticle

Does Herd Immunity Exist in Aquatic Animals?

by Isaac F. Standish¹, Travis O. Brenden² and Mohamed Faisal^1,2,*

¹ Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA

² Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI 48824, USA

Int. J. Mol. Sci. 2016, 17(11), 1898; https://doi.org/10.3390/ijms17111898 - 15 Nov 2016

Cited by 6 | Viewed by 4035

Abstract

Viral hemorrhagic septicemia virus genotype IVb (VHSV-IVb) is presently found throughout the Laurentian Great Lakes region of North America. We recently developed a DNA vaccine preparation containing the VHSV-IVb glycoprotein (G) gene with a cytomegalovirus (CMV) promoter that proved highly efficacious [...] Read more.

Viral hemorrhagic septicemia virus genotype IVb (VHSV-IVb) is presently found throughout the Laurentian Great Lakes region of North America. We recently developed a DNA vaccine preparation containing the VHSV-IVb glycoprotein (G) gene with a cytomegalovirus (CMV) promoter that proved highly efficacious in protecting muskellunge (Esox masquinongy) and three salmonid species. This study was conducted to determine whether cohabitation of VHSV-IVb immunized fishes could confer protection to non-vaccinated (i.e., naïve) fishes upon challenge. The experimental layout consisted of multiple flow-through tanks where viral exposure was achieved via shedding from VHSV-IVb experimentally infected muskellunge housed in a tank supplying water to other tanks. The mean cumulative mortality of naïve muskellunge averaged across eight trials (i.e., replicates) was significantly lower when co-occurring with immunized muskellunge than when naïve muskellunge were housed alone (36.5% when co-occurring with vaccinated muskellunge versus 80.2% when housed alone), indicating a possible protective effect based on cohabitation with vaccinated individuals. Additionally, vaccinated muskellunge when co-occurring with naïve muskellunge had significantly greater anti-VHSV antibody levels compared to vaccinated muskellunge housed alone suggesting that heightened anti-VHSV antibodies are a result of cohabitation with susceptible individuals. This finding could contribute to the considerably lower viable VHSV-IVb concentrations we detected in surviving naive muskellunge when housed with vaccinated muskellunge. Our research provides initial evidence of the occurrence of herd immunity against fish pathogens. Full article

(This article belongs to the Section Biochemistry)

13 pages, 3873 KiB

Open AccessArticle

Berberine Suppresses Cyclin D1 Expression through Proteasomal Degradation in Human Hepatoma Cells

by Ning Wang¹, Xuanbin Wang^2,3, Hor-Yue Tan¹, Sha Li¹, Chi Man Tsang⁴, Sai-Wah Tsao⁴ and Yibin Feng^1,2,3,*

¹ School of Chinese Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong, China

² Laboratory of Chinese Herbal Pharmacology, Oncology Center, Renmin Hospital, Hubei University of Medicine, Shiyan 442000, China

³ Hubei Key Laboratory of Wudang Local Chinese Medicine Research and School of Pharmacy, Hubei University of Medicine, Shiyan 442000, China

⁴ Department of Anatomy, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China

Int. J. Mol. Sci. 2016, 17(11), 1899; https://doi.org/10.3390/ijms17111899 - 15 Nov 2016

Cited by 53 | Viewed by 7797

Abstract

The aim of this study is to explore the underlying mechanism on berberine-induced Cyclin D1 degradation in human hepatic carcinoma. We observed that berberine could suppress both in vitro and in vivo expression of Cyclin D1 in hepatoma cells. Berberine exhibits dose- and [...] Read more.

The aim of this study is to explore the underlying mechanism on berberine-induced Cyclin D1 degradation in human hepatic carcinoma. We observed that berberine could suppress both in vitro and in vivo expression of Cyclin D1 in hepatoma cells. Berberine exhibits dose- and time-dependent inhibition on Cyclin D1 expression in human hepatoma cell HepG2. Berberine increases the phosphorylation of Cyclin D1 at Thr286 site and potentiates Cyclin D1 nuclear export to cytoplasm for proteasomal degradation. In addition, berberine recruits the Skp, Cullin, F-box containing complex-β-Transducin Repeat Containing Protein (SCF^β-TrCP) complex to facilitate Cyclin D1 ubiquitin-proteasome dependent proteolysis. Knockdown of β-TrCP blocks Cyclin D1 turnover induced by berberine; blocking the protein degradation induced by berberine in HepG2 cells increases tumor cell resistance to berberine. Our results shed light on berberine′s potential as an anti-tumor agent for clinical cancer therapy. Full article

(This article belongs to the Special Issue Biological Activity of Natural Secondary Metabolite Products)

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11 pages, 1164 KiB

Open AccessArticle

A Novel Inflammation-Based Stage (I Stage) Predicts Overall Survival of Patients with Nasopharyngeal Carcinoma

by Jian-Pei Li^†, Shu-Lin Chen^†, Xiao-Min Liu, Xia He, Shan Xing, Yi-Jun Liu, Yue-Hao Lin^* and Wan-Li Liu^*

¹ State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Department of Clinical Laboratory Medicine, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1900; https://doi.org/10.3390/ijms17111900 - 15 Nov 2016

Cited by 30 | Viewed by 4730

Abstract

Recent studies have indicated that inflammation-based prognostic scores, such as the Glasgow Prognostic Score (GPS), modified GPS (mGPS) and C-reactive protein/Albumin (CRP/Alb) ratio, platelet–lymphocyte ratio (PLR), and neutrophil–lymphocyte ratio (NLR), have been reported to have prognostic value in patients with many types of [...] Read more.

Recent studies have indicated that inflammation-based prognostic scores, such as the Glasgow Prognostic Score (GPS), modified GPS (mGPS) and C-reactive protein/Albumin (CRP/Alb) ratio, platelet–lymphocyte ratio (PLR), and neutrophil–lymphocyte ratio (NLR), have been reported to have prognostic value in patients with many types of cancer, including nasopharyngeal carcinoma (NPC). In this study, we proposed a novel inflammation-based stage, named I stage, for patients with NPC. A retrospective study of 409 newly-diagnosed cases of NPC was conducted. The prognostic factors (GPS, mGPS, CRP/Alb ratios, PLR, and NLR) were evaluated using univariate and multivariate analyses. Then, according to the results of the multivariate analyses, we proposed a I stage combination of independent risk factors (CRP/Alb ratio and PLR). The I stage was calculated as follows: patients with high levels of CRP/Alb ratio (>0.03) and PLR (>146.2) were defined as I2; patients with one or no abnormal values were defined as I1 or I0, respectively. The relationships between the I stage and clinicopathological variables and overall survival (OS) were evaluated. In addition, the discriminatory ability of the I stage with other inflammation-based prognostic scores was assessed using the AUCs (areas under the curves) analyzed by receiver operating characteristics (ROC) curves. The p value of <0.05 was considered to be significant. A total of 409 patients with NPC were enrolled in this study. Multivariate analyses revealed that only the CRP/Alb ratio (Hazard ratio (HR) = 2.093; 95% Confidence interval (CI): 1.222–3.587; p = 0.007) and PLR (HR: 2.003; 95% CI: 1.177–3.410; p = 0.010) were independent prognostic factors in patients with NPC. The five-year overall survival rates for patients with I0, I1, and I2 were 92.1% ± 2.9%, 83.3% ± 2.6%, and 63.1% ± 4.6%, respectively (p < 0.001). The I stage had a higher area under the curve value (0.670) compared with other systemic inflammation-based prognostic scores (p < 0.001). The I stage is a novel and useful predictive factor for OS in patients with NPC. Full article

(This article belongs to the Special Issue Molecular Classification of Human Cancer: Diagnosis and Treatment 2016)

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12 pages, 709 KiB

Open AccessReview

Antiviral Therapy by HIV-1 Broadly Neutralizing and Inhibitory Antibodies

by Zhiqing Zhang¹, Shaowei Li^1,2, Ying Gu^1,2,* and Ningshao Xia^1,2

¹ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China

² National Institute of Diagnostics and Vaccine Development in Infectious Disease, School of Life Sciences, Xiamen University, Xiamen 361102, China

Int. J. Mol. Sci. 2016, 17(11), 1901; https://doi.org/10.3390/ijms17111901 - 18 Nov 2016

Cited by 14 | Viewed by 10698

Abstract

Human immunodeficiency virus type 1 (HIV-1) infection causes acquired immune deficiency syndrome (AIDS), a global epidemic for more than three decades. HIV-1 replication is primarily controlled through antiretroviral therapy (ART) but this treatment does not cure HIV-1 infection. Furthermore, there is increasing viral [...] Read more.

Human immunodeficiency virus type 1 (HIV-1) infection causes acquired immune deficiency syndrome (AIDS), a global epidemic for more than three decades. HIV-1 replication is primarily controlled through antiretroviral therapy (ART) but this treatment does not cure HIV-1 infection. Furthermore, there is increasing viral resistance to ART, and side effects associated with long-term therapy. Consequently, there is a need of alternative candidates for HIV-1 prevention and therapy. Recent advances have discovered multiple broadly neutralizing antibodies against HIV-1. In this review, we describe the key epitopes on the HIV-1 Env protein and the reciprocal broadly neutralizing antibodies, and discuss the ongoing clinical trials of broadly neutralizing and inhibitory antibody therapy as well as antibody combinations, bispecific antibodies, and methods that improve therapeutic efficacy by combining broadly neutralizing antibodies (bNAbs) with latency reversing agents. Compared with ART, HIV-1 therapeutics that incorporate these broadly neutralizing and inhibitory antibodies offer the advantage of decreasing virus load and clearing infected cells, which is a promising prospect in HIV-1 prevention and treatment. Full article

(This article belongs to the Special Issue Immuno- and Neuropathogenesis of HIV Disease: Mechanisms, Prevention, Treatment, and Cure)

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12 pages, 3616 KiB

Open AccessArticle

Ziyuglycoside I Inhibits the Proliferation of MDA-MB-231 Breast Carcinoma Cells through Inducing p53-Mediated G2/M Cell Cycle Arrest and Intrinsic/Extrinsic Apoptosis

by Xue Zhu^1,†, Ke Wang^1,†, Kai Zhang¹, Ting Zhang^2,3, Yongxiang Yin⁴ and Fei Xu^5,*

¹ Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China

² The Affiliated Maternity and Children Health Hospital of Nanjing Medical University, Wuxi 214002, China

³ Department of Molecular Cell Biology and Toxicology, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment Cancer Center, School of Public Health, Nanjing Medical University, Nanjing 210029, China

⁴ Department of Pathology, the Affiliated Maternity and Children Health Hospital of Nanjing Medical University, Wuxi 214002, China

⁵ Department of Laboratory Medicine, the Affiliated Maternity and Children Health Hospital of Nanjing Medical University, Wuxi 214002, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1903; https://doi.org/10.3390/ijms17111903 - 22 Nov 2016

Cited by 31 | Viewed by 6607

Abstract

Background: Due to the aggressive clinical behavior, poor outcome, and lack of effective specific targeted therapies, triple-negative breast cancer (TNBC) has currently been recognized as one of the most malignant types of tumors. In the present study, we investigated the cytotoxic effect of [...] Read more.

Background: Due to the aggressive clinical behavior, poor outcome, and lack of effective specific targeted therapies, triple-negative breast cancer (TNBC) has currently been recognized as one of the most malignant types of tumors. In the present study, we investigated the cytotoxic effect of ziyuglycoside I, one of the major components extracted from Chinese anti-tumor herbal Radix Sanguisorbae, on the TNBC cell line MDA-MB-231. Methods: The underlying molecular mechanism of the cytotoxic effect ziyuglycoside I on MDA-MB-231 cells was investigated with cell viability assay, flow cytometric analysis and Western blot. Results: Compared to normal mammary gland Hs 578Bst cells, treatment of ziyuglycoside I resulted in a significant growth inhibitory effect on MDA-MB-231 cells. Ziyuglycoside I induced the G2/M phase arrest and apoptosis of MDA-MB-231 cells in a dose-dependent manner. These effects were found to be partially mediated through the up-regulation of p53 and p21^WAF1, elevated Bax/Bcl-2 ratio, and the activation of both intrinsic (mitochondrial-initiated) and extrinsic (Fas/FasL-initiated) apoptotic pathways. Furthermore, the p53 specific siRNA attenuated these effects. Conclusion: Our study suggested that ziyuglycoside I-triggered MDA-MB-231 cell cycle arrest and apoptosis were probably mediated by p53. This suggests that ziyuglycoside I might be a potential drug candidate for treating TNBC. Full article

(This article belongs to the Section Molecular Toxicology)

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15 pages, 1851 KiB

Open AccessArticle

Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium Hydroxide-Induced Vibrio parahaemolyticus Ghosts (VPGs)

by Hyun Jung Park^1,†, Sung Oh^1,†, Nagarajan Vinod¹, Seongmi Ji¹, Han Byul Noh¹, Jung Mo Koo¹, Su Hyeong Lee², Sei Chang Kim¹, Ki-Sung Lee¹ and Chang Won Choi^1,*

¹ Department of Biology & Medicinal Science, Pai Chai University, Daejeon 35345, Korea

² Cheongshim International Academy, Gapyeong-gun, Gyeonggi-do 12461, Korea

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1904; https://doi.org/10.3390/ijms17111904 - 15 Nov 2016

Cited by 46 | Viewed by 8764

Abstract

Acellular bacterial ghosts (BGs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene E of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were generated by chemically-induced lysis and the method is based on minimum [...] Read more.

Acellular bacterial ghosts (BGs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene E of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were generated by chemically-induced lysis and the method is based on minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH), acetic acid, boric acid, citric acid, maleic acid, hydrochloric acid, and sulfuric acid. The MIC values of the respective chemicals were 3.125, 6.25, <50.0, 25.0, 6.25, 1.56, and 0.781 mg/mL. Except for boric acid, the lysis efficiency reached more than 99.99% at 5 min after treatment of all chemicals. Among those chemicals, NaOH-induced VPGs appeared completely DNA-free, which was confirmed by quantitative real-time PCR. Besides, lipopolysaccharides (LPS) extracted from the NaOH-induced VPGs showed no distinctive band on SDS-PAGE gel after silver staining. On the other hand, LPS extracted from wild-type bacterial cells, as well as the organic acids-induced VPGs showed triple major bands and LPS extracted from the inorganic acids-induced VPGs showed double bands. It suggests that some surface structures in LPS of the NaOH-induced VPGs may be lost, weakened, or modified by the MIC of NaOH. Nevertheless, Limulus amoebocyte lysate assay revealed that there is no significant difference in endotoxic activity between the NaOH-induced VPGs and wild-type bacterial cells. Macrophages exposed to the NaOH-induced VPGs at 0.5 × 10⁶ CFU/mL showed cell viability of 97.9%, however, the MIC of NaOH did not reduce the cytotoxic effect of wild-type bacterial cells. Like Escherichia coli LPS, the NaOH-induced VPGs are an excellent activator of pro-inflammatory cytokines (IL-1β and iNOS), anti-inflammatory cytokine (IL-10), and dual activities (IL-6) in the stimulated macrophage cells. On the other hand, the induction of TNF-α mRNA was remarkable in the macrophages exposed with wild-type cells. Scanning electron microscopy showed the formation of trans-membrane lysis tunnel structures in the NaOH-induced VPGs. SDS-PAGE and agarose gel electrophoresis also confirmed that cytoplasmic proteins and genomic DNA released from the VPGs to culture medium through the lysis tunnel structures. Taken together, all these data indicate that the NaOH-induced VPGs show the potency of a safe, economical, and effective inactivated bacterial vaccine candidate. Full article

(This article belongs to the Special Issue Advances in Cheap Vaccines for Public Goods)

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11 pages, 13003 KiB

Open AccessCommunication

Mast Cells Density Positive to Tryptase Correlate with Microvascular Density in both Primary Gastric Cancer Tissue and Loco-Regional Lymph Node Metastases from Patients That Have Undergone Radical Surgery

by Michele Ammendola^1,2,*

, Rosario Sacco¹, Valeria Zuccalà³, Maria Luposella⁴, Rosa Patruno⁵, Pietro Gadaleta⁶, Nicola Zizzo⁵

, Cosmo Damiano Gadaleta⁶, Giovambattista De Sarro⁷, Giuseppe Sammarco¹, Mihai Oltean⁸

and Girolamo Ranieri⁶

¹ Department of Medical and Surgical Sciences, Clinical Surgery Unit, University “Magna Graecia” Medical School, Viale Europa, Germaneto, 88100 Catanzaro, Italy

² Surgery Unit, National Cancer Research Centre, Istituto Tumori “Giovanni Paolo II”, Viale Orazio Flacco 65, 70124 Bari, Italy

³ Pathology Unit, “Pugliese-Ciaccio” Hospital, Viale Pio X, 88100 Catanzaro, Italy

⁴ Cardiovascular Disease Unit, “San Giovanni di Dio” Hospital, 88900 Crotone, Italy

⁵ Chair of Pathology, Veterinary Medical School, University “Aldo Moro” of Bari, Via Casamassima, 70010 Bari, Italy

⁶ Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology, National Cancer Research Centre, Istituto Tumori “Giovanni Paolo II”, viale Orazio Flacco 65, 70124 Bari, Italy

⁷ Department of Health Science, Clinical Pharmacology and Pharmacovigilance Unit and Pharmacovigilance’s Centre Calabria Region, University of Catanzaro “Magna Graecia” Medical School, Viale Europa, Germaneto, 88100 Catanzaro, Italy

⁸ The Institute for Clinical Sciences, Department of Transplantation, University Hospital, Sahlgrenska Academy at the University of Gothenburg, 41345 Gothenburg, Sweden

Int. J. Mol. Sci. 2016, 17(11), 1905; https://doi.org/10.3390/ijms17111905 - 15 Nov 2016

Cited by 28 | Viewed by 6330

Abstract

Mast Cells (MCs) play a role in immune responses and more recently MCs have been involved in tumoral angiogenesis. In particular MCs can release tryptase, a potent in vivo and in vitro pro-angiogenic factor via proteinase-activated receptor-2 (PAR-2) activation and mitogen-activated protein kinase [...] Read more.

Mast Cells (MCs) play a role in immune responses and more recently MCs have been involved in tumoral angiogenesis. In particular MCs can release tryptase, a potent in vivo and in vitro pro-angiogenic factor via proteinase-activated receptor-2 (PAR-2) activation and mitogen-activated protein kinase (MAPK) phosphorylation. MCs can release tryptase following c-Kit receptor activation. Nevertheless, no data are available concerning the relationship among MCs Density Positive to Tryptase (MCDPT) and Microvascular Density (MVD) in both primary gastric cancer tissue and loco-regional lymph node metastases. A series of 75 GC patients with stage T_2–3N_2–3M₀ (by AJCC for Gastric Cancer Seventh Edition) undergone to radical surgery were selected for the study. MCDPT and MVD were evaluated by immunohistochemistry and by image analysis system and results were correlated each to other in primary tumor tissue and in metastatic lymph nodes harvested. Furthermore, tissue parameters were correlated with important clinico-pathological features. A significant correlation between MCDPT and MVD was found in primary gastric cancer tissue and lymph node metastases. Pearson t-test analysis (r ranged from 0.74 to 0.79; p-value ranged from 0.001 to 0.003). These preliminary data suggest that MCDPT play a role in angiogenesis in both primary tumor and in lymph node metastases from GC. We suggest that MCs and tryptase could be further evaluated as novel targets for anti-angiogenic therapies. Full article

(This article belongs to the Special Issue Vascular Biology and Therapeutics)

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19 pages, 6866 KiB

Open AccessReview

An Overview of Hardware for Protein Crystallization in a Magnetic Field

by Er-Kai Yan

, Chen-Yan Zhang, Jin He and Da-Chuan Yin^*

Institute for Special Environmental Biophysics, Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China

Int. J. Mol. Sci. 2016, 17(11), 1906; https://doi.org/10.3390/ijms17111906 - 16 Nov 2016

Cited by 23 | Viewed by 6172

Abstract

Protein crystallization under a magnetic field is an interesting research topic because a magnetic field may provide a special environment to acquire improved quality protein crystals. Because high-quality protein crystals are very useful in high-resolution structure determination using diffraction techniques (X-ray, neutron, and [...] Read more.

Protein crystallization under a magnetic field is an interesting research topic because a magnetic field may provide a special environment to acquire improved quality protein crystals. Because high-quality protein crystals are very useful in high-resolution structure determination using diffraction techniques (X-ray, neutron, and electron diffraction), research using magnetic fields in protein crystallization has attracted substantial interest; some studies have been performed in the past two decades. In this research field, the hardware is especially essential for successful studies because the environment is special and the design and utilization of the research apparatus in such an environment requires special considerations related to the magnetic field. This paper reviews the hardware for protein crystallization (including the magnet systems and the apparatus designed for use in a magnetic field) and progress in this area. Future prospects in this field will also be discussed. Full article

(This article belongs to the Section Biochemistry)

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11 pages, 12652 KiB

Open AccessArticle

Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice

by Kota Kataoka¹, Daisuke Ekuni^1,*

, Takaaki Tomofuji^1,2, Koichiro Irie¹, Muneyoshi Kunitomo¹, Yoko Uchida¹, Daiki Fukuhara¹ and Manabu Morita¹

¹ Departments of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan

² Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan

Int. J. Mol. Sci. 2016, 17(11), 1907; https://doi.org/10.3390/ijms17111907 - 16 Nov 2016

Cited by 27 | Viewed by 5118

Abstract

The aim of this study was to investigate whether a Keap1-dependent oxidative stress detector-luciferase (OKD-LUC) mouse model would be useful for the visualization of oxidative stress induced by experimental periodontitis. A ligature was placed around the mandibular first molars for seven days to [...] Read more.

The aim of this study was to investigate whether a Keap1-dependent oxidative stress detector-luciferase (OKD-LUC) mouse model would be useful for the visualization of oxidative stress induced by experimental periodontitis. A ligature was placed around the mandibular first molars for seven days to induce periodontitis. Luciferase activity was measured with an intraperitoneal injection of d-luciferin on days 0, 1, and 7. The luciferase activity in the periodontitis group was significantly greater than that in the control group at seven days. The expressions of heme oxygenase-1 (HO-1) and malondialdehyde in periodontal tissue were significantly higher in the periodontitis group than in the control group. Immunofluorescent analysis confirmed that the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) occurred more frequently in the periodontitis group than in the control group. This study found that under oxidative stress induced by experimental periodontitis, the Nrf2/antioxidant defense pathway was activated and could be visualized from the luciferase activity in the OKD-LUC model. Thus, the OKD-LUC mouse model may be useful for exploring the mechanism underlying the relationship between the Nrf2/antioxidant defense pathway and periodontitis by enabling the visualization of oxidative stress over time. Full article

(This article belongs to the Section Biochemistry)

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17 pages, 2886 KiB

Open AccessArticle

miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1

by Zhi Chen^1,†, Huiling Qiu^2,†, Liuan Ma^2,†, Jun Luo^1,*, Shuang Sun¹, Kang Kang², Deming Gou^2,* and Juan J. Loor³

¹ Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China

² College of Life Sciences, Shenzhen University, Shenzhen 518060, China

³ Mammalian NutriPhysioGenomics, Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1909; https://doi.org/10.3390/ijms17111909 - 16 Nov 2016

Cited by 47 | Viewed by 5909

Abstract

MicroRNA (miRNA) regulates the expression of genes and influences a series of biological processes, including fatty acid metabolism. We screened the expression of miRNA in goat mammary glands during peak-lactation and non-lactating (“dry”) periods, and performed an in vitro study with goat mammary [...] Read more.

MicroRNA (miRNA) regulates the expression of genes and influences a series of biological processes, including fatty acid metabolism. We screened the expression of miRNA in goat mammary glands during peak-lactation and non-lactating (“dry”) periods, and performed an in vitro study with goat mammary epithelial cells (GMEC) prior to sequencing analysis. Results illustrated that miR-30e-5p and miR-15a were highly expressed. Utilizing a luciferase reporter assay and Western blot, low-density lipoprotein receptor-related protein 6 (LRP6) and Yes associated protein 1 (YAP1) genes were demonstrated to be a target of miR-30e-5p and miR-15a in GMEC. Moreover, we demonstrated that the overexpression of miR-30e-5p and miR-15a in GMEC promoted fat metabolism while their knockdown impaired fat metabolism. These findings extend the discovery of a key role of miR-30e-5p and miR-15a in mediating adipocyte differentiation by suggesting a role in promoting milk fat synthesis. In conclusion, our findings indicate that miR-30e-5p, together with miR-15a, represses expression of LRP6 and promotes fat metabolism in GMEC. The data expanded our knowledge on the function of miRNAs in milk fat metabolism and synthesis in ruminant mammary cells. Full article

(This article belongs to the Special Issue microRNA Regulation 2017)

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14 pages, 1012 KiB

Open AccessReview

Tension in Cancer

by Stefanie Löffek^1,2,*, Claus-Werner Franzke³ and Iris Helfrich^1,2

¹ Skin Cancer Unit of the Dermatology Department, Medical Faculty, West German Cancer Center, University Duisburg-Essen, 45147 Essen, Germany

² German Cancer Consortium (DKTK), University Duisburg-Essen, 45147 Essen, Germany

³ Department of Dermatology and Venerology, Medical Center, University of Freiburg, Hauptstraße 7, 79104 Freiburg, Germany

Int. J. Mol. Sci. 2016, 17(11), 1910; https://doi.org/10.3390/ijms17111910 - 16 Nov 2016

Cited by 15 | Viewed by 7472

Abstract

Integrins represent a large family of cell receptors that mediate adhesion to the extracellular matrix (ECM), thereby modulating a variety of cellular functions that are required for proliferation, migration, malignant conversion and invasiveness. During tumorigenesis the conversion of a tumor cell from sessile, [...] Read more.

Integrins represent a large family of cell receptors that mediate adhesion to the extracellular matrix (ECM), thereby modulating a variety of cellular functions that are required for proliferation, migration, malignant conversion and invasiveness. During tumorigenesis the conversion of a tumor cell from sessile, stationary phenotype to an invasive phenotype requires the ability of tumor cells to interact with their environment in order to transduce signals from the ECM into the cells. Hence, there is increasing evidence that changes in the composition, topography and tension of tumor matrix can be sensed by integrin receptors, leading to the regulation of intracellular signalling events which subsequently help to fuel cancer progression. The fact that intracellular signals perceived from integrin ligand binding impact on almost all steps of tumor progression, including tumor cell proliferation, survival, metastatic dissemination and colonization of a metastatic niche, renders integrins as ideal candidates for the development of therapeutic agents. In this review we summarize the role of integrins in cancer with the special focus on cancer therapies and the recent progress that has been made in the understanding of “integrin-induced tension in cancer”. Finally, we conclude with clinical evidence for the role of integrin-mediated mechanotransduction in the development of therapy-resistant tumors. Full article

(This article belongs to the Special Issue Integrins in Cancer)

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10 pages, 1837 KiB

Open AccessArticle

SEPT12–NDC1 Complexes Are Required for Mammalian Spermiogenesis

by Tsung-Hsuan Lai^1,2,3, Ying-Yu Wu⁴, Ya-Yun Wang⁵, Mei-Feng Chen⁶, Pei Wang², Tsung-Ming Chen⁷, Yi-No Wu⁴, Han-Sun Chiang⁴, Pao-Lin Kuo⁸ and Ying-Hung Lin^4,*

¹ Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei 106, Taiwan

² School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan

³ Institute of Systems Biology and Bioinformatics, National Central University, Jhongli City, Taoyuan Country 320, Taiwan

⁴ Graduate Institute of Biomedical and Pharmaceutical Science, Fu Jen Catholic University, New Taipei City 242, Taiwan

⁵ Department of Chemistry, Fu Jen Catholic University, New Taipei City 242, Taiwan

⁶ Bone and Joint Research Center, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan

⁷ Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung 811, Taiwan

⁸ Department of Obstetrics & Gynecology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan

Int. J. Mol. Sci. 2016, 17(11), 1911; https://doi.org/10.3390/ijms17111911 - 16 Nov 2016

Cited by 20 | Viewed by 5935

Abstract

Male factor infertility accounts for approximately 50 percent of infertile couples. The male factor-related causes of intracytoplasmic sperm injection failure include the absence of sperm, immotile sperm, immature sperm, abnormally structured sperm, and sperm with nuclear damage. Our knockout and knock-in mice models [...] Read more.

Male factor infertility accounts for approximately 50 percent of infertile couples. The male factor-related causes of intracytoplasmic sperm injection failure include the absence of sperm, immotile sperm, immature sperm, abnormally structured sperm, and sperm with nuclear damage. Our knockout and knock-in mice models demonstrated that SEPTIN12 (SEPT12) is vital for the formation of sperm morphological characteristics during spermiogenesis. In the clinical aspect, mutated SEPT12 in men results in oligozoospermia or teratozoospermia or both. Sperm with mutated SEPT12 revealed abnormal head and tail structures, decreased chromosomal condensation, and nuclear damage. Furthermore, several nuclear or nuclear membrane-related proteins have been identified as SEPT12 interactors through the yeast 2-hybrid system, including NDC1 transmembrane nucleoporin (NDC1). NDC1 is a major nuclear pore protein, and is critical for nuclear pore complex assembly and nuclear morphology maintenance in mammalian cells. Mutated NDC1 cause gametogenesis defects and skeletal malformations in mice, which were detected spontaneously in the A/J strain. In this study, we characterized the functional effects of SEPT12–NDC1 complexes during mammalian spermiogenesis. In mature human spermatozoa, SEPT12 and NDC1 are majorly colocalized in the centrosome regions; however, NDC1 is only slightly co-expressed with SEPT12 at the annulus of the sperm tail. In addition, SEPT12 interacts with NDC1 in the male germ cell line through coimmunoprecipitation. During murine spermiogenesis, we observed that NDC1 was located at the nuclear membrane of spermatids and at the necks of mature spermatozoa. In male germ cell lines, NDC1 overexpression restricted the localization of SEPT12 to the nucleus and repressed the filament formation of SEPT12. In mice sperm with mutated SEPT12, NDC1 dispersed around the manchette region of the sperm head and annulus, compared with concentrating at the sperm neck of wild-type sperm. These results indicate that SEPT12–NDC1 complexes are involved in mammalian spermiogenesis. Full article

(This article belongs to the Section Biochemistry)

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16 pages, 3916 KiB

Open AccessArticle

Enhanced Production of Anthraquinones and Phenolic Compounds and Biological Activities in the Cell Suspension Cultures of Polygonum multiflorum

by Muthu Thiruvengadam

, Kaliyaperumal Rekha, Govindasamy Rajakumar, Taek-Jun Lee, Seung-Hyun Kim and Ill-Min Chung^*

Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 143 701, Korea

Int. J. Mol. Sci. 2016, 17(11), 1912; https://doi.org/10.3390/ijms17111912 - 16 Nov 2016

Cited by 41 | Viewed by 5952

Abstract

Anthraquinones (AQs) and phenolic compounds are important phytochemicals that are biosynthesized in cell suspension cultures of Polygonum multiflorum. We wanted to optimize the effects of plant growth regulators (PGRs), media, sucrose, l-glutamine, jasmonic acid (JA), and salicylic acid (SA) for the [...] Read more.

Anthraquinones (AQs) and phenolic compounds are important phytochemicals that are biosynthesized in cell suspension cultures of Polygonum multiflorum. We wanted to optimize the effects of plant growth regulators (PGRs), media, sucrose, l-glutamine, jasmonic acid (JA), and salicylic acid (SA) for the production of phytochemicals and biomass accumulation in a cell suspension culture of P. multiflorum. The medium containing Murashige and Skoog (MS) salts and 4% sucrose supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid, 0.5 mg/L thidiazuron, and 100 µM l-glutamine at 28 days of cell suspension culture was suitable for biomass accumulation and AQ production. Maximum biomass accumulation (12.5 and 12.35 g fresh mass (FM); 3 and 2.93 g dry mass (DM)) and AQ production (emodin 295.20 and 282 mg/g DM; physcion 421.55 and 410.25 mg/g DM) were observed using 100 µM JA and SA, respectively. JA- and SA-elicited cell cultures showed several-fold higher biomass accumulation and AQ production than the control cell cultures. Furthermore, the cell suspension cultures effectively produced 23 phenolic compounds, such as flavonols and hydroxycinnamic and hydroxybenzoic acid derivatives. PGR-, JA-, and SA-elicited cell cultures produced a higher amount of AQs and phenolic compounds. Because of these metabolic changes, the antioxidant, antimicrobial, and anticancer activities were high in the PGR-, JA-, and SA-elicited cell cultures. The results showed that the elicitors (JA and SA) induced the enhancement of biomass accumulation and phytochemical (AQs and phenolic compounds) production as well as biological activities in the cell suspension cultures of P. multiflorum. This optimized protocol can be developed for large-scale biomass accumulation and production of phytochemicals (AQs and phenolic compounds) from cell suspension cultures, and the phytochemicals can be used for various biological activities. Full article

(This article belongs to the Section Bioactives and Nutraceuticals)

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12 pages, 543 KiB

Open AccessArticle

Characterizing Non-Tuberculous Mycobacteria Infection in Bronchiectasis

by Paola Faverio¹, Anna Stainer¹, Giulia Bonaiti¹, Stefano C. Zucchetti¹, Edoardo Simonetta¹, Giuseppe Lapadula², Almerico Marruchella¹

, Andrea Gori²

, Francesco Blasi³

, Luigi Codecasa⁴, Alberto Pesci¹, James D. Chalmers⁵, Michael R. Loebinger⁶ and Stefano Aliberti^3,*

¹ Dipartimento Cardio-Toraco-Vascolare, University of Milan Bicocca, Respiratory Unit, San Gerardo Hospital, ASST di Monza, Via Pergolesi 33, 20900 Monza, Italy

² Department of Internal Medicine, Division of Infectious Diseases, San Gerardo Hospital, ASST di Monza, Via Pergolesi 33, 20900 Monza, Italy

³ Department of Pathophysiology and Transplantation, University of Milan, Cardio-Thoracic Unit and Cystic Fibrosis Adult Center, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, Italy

⁴ Villa Marelli Institute, Niguarda Ca’ Granda Hospital, 20122 Milan, Italy

⁵ Scottish Centre for Respiratory Research, University of Dundee, Dundee DD1 9SY, UK

⁶ Host Defence Unit, Royal Brompton and Harefield NHS Foundation Trust, London, UK Imperial College London, London SW3 6NP, UK

Int. J. Mol. Sci. 2016, 17(11), 1913; https://doi.org/10.3390/ijms17111913 - 16 Nov 2016

Cited by 76 | Viewed by 7127

Abstract

Chronic airway infection is a key aspect of the pathogenesis of bronchiectasis. A growing interest has been raised on non-tuberculous mycobacteria (NTM) infection. We aimed at describing the clinical characteristics, diagnostic process, therapeutic options and outcomes of bronchiectasis patients with pulmonary NTM (pNTM) [...] Read more.

Chronic airway infection is a key aspect of the pathogenesis of bronchiectasis. A growing interest has been raised on non-tuberculous mycobacteria (NTM) infection. We aimed at describing the clinical characteristics, diagnostic process, therapeutic options and outcomes of bronchiectasis patients with pulmonary NTM (pNTM) disease. This was a prospective, observational study enrolling 261 adult bronchiectasis patients during the stable state at the San Gerardo Hospital, Monza, Italy, from 2012 to 2015. Three groups were identified: pNTM disease; chronic P. aeruginosa infection; chronic infection due to bacteria other than P. aeruginosa. NTM were isolated in 32 (12%) patients, and among them, a diagnosis of pNTM disease was reached in 23 cases. When compared to chronic P. aeruginosa infection, patients with pNTM were more likely to have cylindrical bronchiectasis and a “tree-in-bud” pattern, a history of weight loss, a lower disease severity and a lower number of pulmonary exacerbations. Among pNTM patients who started treatment, 68% showed a radiological improvement, and 37% achieved culture conversion without recurrence, while 21% showed NTM isolation recurrence. NTM isolation seems to be a frequent event in bronchiectasis patients, and few parameters might help to suspect NTM infection. Treatment indications and monitoring still remain an important area for future research. Full article

(This article belongs to the Special Issue Lung Diseases: Chronic Respiratory Infections)

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2 pages, 151 KiB

Open AccessEditorial

Editorial: Analysis of the Interaction of Dp44mT with Human Serum Albumin and Calf Thymus DNA Using Molecular Docking and Spectroscopic Techniques

by Gregor P. C. Drummen^1,*,† and Christo Z. Christov^2,*,†

¹ Cellular Stress and Ageing Program, Hepato and Renal Pathobiology Program, Bio&Nano Solutions, D-33647 Bielefeld, Germany

² Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne NE1 8ST, UK

^† Editorial Board of the International Journal of the Molecular Sciences, MDPI AG, Basel, Switzerland.

Int. J. Mol. Sci. 2016, 17(11), 1914; https://doi.org/10.3390/ijms17111914 - 16 Nov 2016

Viewed by 2906

Abstract

This Editorial refers to: Full article

4 pages, 1613 KiB

Open AccessCorrection

Correction: Zhongjie Xu, et al. Analysis of the Interaction of Dp44mT with Human Serum Albumin and Calf Thymus DNA using Molecular Docking and Spectroscopic Techniques. Int. J. Mol. Sci. 2016, 17, 1042

by Zhongjie Xu^1,†, Youxun Liu^2,†, Sufeng Zhou², Yun Fu² and Changzheng Li^2,3,*

¹ College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China

² Department of Molecular Biology & Biochemistry, Xinxiang Medical University, Xinxiang 453003, China

³ Henan Collaborative Innovation Center of Molecular Diagnostics and Laboratory Medicine, Xinxiang 453003, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1915; https://doi.org/10.3390/ijms17111915 - 16 Nov 2016

Cited by 4 | Viewed by 3469

Abstract

n/a Full article

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2 pages, 167 KiB

Open AccessLetter

Letter to the Editor: “Analysis of the Interaction of Dp44mT with Human Serum Albumin and Calf Thymus DNA Using Molecular Docking and Spectroscopic Techniques”

by Angelica M. Merlot, Sumit Sahni, Darius J. R. Lane, Vera Richardson, Michael L. H. Huang, Danuta S. Kalinowski and Des R. Richardson^*

Department of Pathology and Bosch Institute, University of Sydney, Sydney 2006, Australia

Int. J. Mol. Sci. 2016, 17(11), 1916; https://doi.org/10.3390/ijms17111916 - 16 Nov 2016

Cited by 4 | Viewed by 3326

Abstract

In reading the article by Xu, Z., et al. entitled “Analysis of the Interaction of Dp44mT with Human Serum Albumin and Calf Thymus DNA Using Molecular Docking and Spectroscopic Techniques” [1], there is evidence that some of the key methods and comparisons utilized [...] Read more.

In reading the article by Xu, Z., et al. entitled “Analysis of the Interaction of Dp44mT with Human Serum Albumin and Calf Thymus DNA Using Molecular Docking and Spectroscopic Techniques” [1], there is evidence that some of the key methods and comparisons utilized in this study are non-optimal and problematic[...] Full article

5 pages, 190 KiB

Open AccessReply

Response to the Letter to the Editor by D. Richardson: Analysis of the Interaction of Dp44mT with Human Serum Albumin and Calf Thymus DNA Using Molecular Docking and Spectroscopic Techniques

by Zhongjie Xu¹, Youxun Liu², Sufeng Zhou², Yun Fu² and Changzheng Li^2,3,*

¹ College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China

² Department of Molecular Biology & Biochemistry, Xinxiang Medical University, Xinxiang 453003, China

³ Henan Collaborative Innovation Center of Molecular Diagnostics and Laboratory Medicine, Xinxiang 453003, China

Int. J. Mol. Sci. 2016, 17(11), 1917; https://doi.org/10.3390/ijms17111917 - 16 Nov 2016

Cited by 2 | Viewed by 2969

Abstract

This response refers to: Full article

2 pages, 143 KiB

Open AccessEditorial

Translational Medicine: Creating the Crucial Bidirectional Bridge between Bench and Bedside

by Xiaofeng Jia^1,2,3,4,5

¹ Department of Neurosurgery, University of Maryland School of Medicine, Baltimore, MD 21201, USA

² Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21201, USA

³ Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA

⁴ Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

⁵ Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

Int. J. Mol. Sci. 2016, 17(11), 1918; https://doi.org/10.3390/ijms17111918 - 16 Nov 2016

Cited by 7 | Viewed by 3418

Abstract

The life sciences are now entering a revolutionary era that calls for acceleration in understanding the complexity of biological systems and expands the need for more effective strategies for pursuing translational medicine [1].[...] Full article

(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)

16 pages, 2330 KiB

Open AccessArticle

A Mixture of Extracts of Kochia scoparia and Rosa multiflora with PPAR α/γ Dual Agonistic Effects Prevents Photoaging in Hairless Mice

by Hyerin Jeon¹, Dong Hye Kim¹, Youn-Hwa Nho²

, Ji-Eun Park², Su-Nam Kim³ and Eung Ho Choi^1,*

¹ Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju 26426, Korea

² Skin Research Team, Cosmax R&I Center, Seongnam 13486, Korea

³ Natural Skinomics Team, KIST Gangneung Institute of Natural Products, Gangneung 25451, Korea

Int. J. Mol. Sci. 2016, 17(11), 1919; https://doi.org/10.3390/ijms17111919 - 16 Nov 2016

Cited by 12 | Viewed by 6628

Abstract

Activation of peroxisome proliferator-activated receptors (PPAR) α/γ is known to inhibit the increases in matrix metalloproteinase (MMP) and reactive oxygen species (ROS) induced by ultraviolet light (UV). Extracts of natural herbs, such as Kochia scoparia and Rosa multiflora, have a PPAR α/γ [...] Read more.

Activation of peroxisome proliferator-activated receptors (PPAR) α/γ is known to inhibit the increases in matrix metalloproteinase (MMP) and reactive oxygen species (ROS) induced by ultraviolet light (UV). Extracts of natural herbs, such as Kochia scoparia and Rosa multiflora, have a PPAR α/γ dual agonistic effect. Therefore, we investigated whether and how they have an antiaging effect on photoaging skin. Eighteen-week-old hairless mice were irradiated with UVA 14 J/cm² and UVB 40 mJ/cm² three times a week for 8 weeks. A mixture of extracts of Kochia scoparia and Rosa multiflora (KR) was topically applied on the dorsal skin of photoaging mice twice a day for 8 weeks. Tesaglitazar, a known PPAR α/γ agonist, and vehicle (propylene glycol:ethanol = 7:3, v/v) were applied as positive and negative controls, respectively. Dermal effects (including dermal thickness, collagen density, dermal expression of procollagen 1 and collagenase 13) and epidermal effects (including skin barrier function, epidermal proliferation, epidermal differentiation, and epidermal cytokines) were measured and compared. In photoaging murine skin, KR resulted in a significant recovery of dermal thickness as well as dermal fibroblasts, although it did not change dermal collagen density. KR increased the expression of dermal transforming growth factor (TGF)-β. The dermal effects of KR were explained by an increase in procollagen 1 expression, induced by TGF-β, and a decrease in MMP-13 expression. KR did not affect basal transepidermal water loss (TEWL) or stratum corneum (SC) integrity, but did decrease SC hydration. It also did not affect epidermal proliferation or epidermal differentiation. KR decreased the expression of epidermal interleukin (IL)-1α. Collectively, KR showed possible utility as a therapeutic agent for photoaging skin, with few epidermal side effects such as epidermal hyperplasia or poor differentiation. Full article

(This article belongs to the Special Issue Inflammatory Skin Conditions)

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11 pages, 7842 KiB

Open AccessArticle

Conjugation with RGD Peptides and Incorporation of Vascular Endothelial Growth Factor Are Equally Efficient for Biofunctionalization of Tissue-Engineered Vascular Grafts

by Larisa V. Antonova¹, Alexander M. Seifalian^2,3, Anton G. Kutikhin^1,*, Victoria V. Sevostyanova¹, Vera G. Matveeva¹, Elena A. Velikanova¹, Andrey V. Mironov¹, Amin R. Shabaev⁴, Tatiana V. Glushkova¹, Evgeniya A. Senokosova¹, Georgiy Yu. Vasyukov¹, Evgeniya O. Krivkina¹, Andrey Yu. Burago¹, Yuliya A. Kudryavtseva¹, Olga L. Barbarash¹ and Leonid S. Barbarash¹

¹ Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, Kemerovo 650002, Russia

² Centre for Nanotechnology and Regenerative Medicine, UCL Division of Surgery and Interventional Science, University College London, UCL Medical School Building, 21 University Street, London WC1E 6AU, UK

³ NanoRegMed Ltd., 20-22 Wenlock Road, London N1 7GU, UK

⁴ Kemerovo Cardiology Dispensary, Sosnovy Boulevard 6, Kemerovo 650002, Russia

Int. J. Mol. Sci. 2016, 17(11), 1920; https://doi.org/10.3390/ijms17111920 - 16 Nov 2016

Cited by 31 | Viewed by 6001

Abstract

The blend of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) has recently been considered promising for vascular tissue engineering. However, it was shown that PHBV/PCL grafts require biofunctionalization to achieve high primary patency rate. Here we compared immobilization of arginine–glycine–aspartic acid (RGD)-containing peptides [...] Read more.

The blend of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) has recently been considered promising for vascular tissue engineering. However, it was shown that PHBV/PCL grafts require biofunctionalization to achieve high primary patency rate. Here we compared immobilization of arginine–glycine–aspartic acid (RGD)-containing peptides and the incorporation of vascular endothelial growth factor (VEGF) as two widely established biofunctionalization approaches. Electrospun PHBV/PCL small-diameter grafts with either RGD peptides or VEGF, as well as unmodified grafts were implanted into rat abdominal aortas for 1, 3, 6, and 12 months following histological and immunofluorescence assessment. We detected CD31⁺/CD34⁺/vWF⁺ cells 1 and 3 months postimplantation at the luminal surface of PHBV/PCL/RGD and PHBV/PCL/VEGF, but not in unmodified grafts, with the further observation of CD31⁺CD34⁻vWF⁺ phenotype. These cells were considered as endothelial and produced a collagen-positive layer resembling a basement membrane. Detection of CD31⁺/CD34⁺ cells at the early stages with subsequent loss of CD34 indicated cell adhesion from the bloodstream. Therefore, either conjugation with RGD peptides or the incorporation of VEGF promoted the formation of a functional endothelial cell layer. Furthermore, both modifications increased primary patency rate three-fold. In conclusion, both of these biofunctionalization approaches can be considered as equally efficient for the modification of tissue-engineered vascular grafts. Full article

(This article belongs to the Special Issue Vascular Biology and Therapeutics)

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15 pages, 3294 KiB

Open AccessArticle

Transcriptome-Based Discovery of Fusarium graminearum Stress Responses to FgHV1 Infection

by Shuangchao Wang^1,2,†, Jingze Zhang^1,†, Pengfei Li¹, Dewen Qiu¹ and Lihua Guo^1,*

¹ State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100081, China

² Walloon Centre of Industrial Biology, Gembloux Agro-Bio Tech, University of Liège, Passage des Déportés, 2, Gembloux 5030, Belgium

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1922; https://doi.org/10.3390/ijms17111922 - 17 Nov 2016

Cited by 15 | Viewed by 5567

Abstract

Fusarium graminearum hypovirus 1 (FgHV1), which is phylogenetically related to Cryphonectria hypovirus 1 (CHV1), is a virus in the family Hypoviridae that infects the plant pathogenic fungus F. graminearum. Although hypovirus FgHV1 infection does not attenuate the virulence of the host (hypovirulence), [...] Read more.

Fusarium graminearum hypovirus 1 (FgHV1), which is phylogenetically related to Cryphonectria hypovirus 1 (CHV1), is a virus in the family Hypoviridae that infects the plant pathogenic fungus F. graminearum. Although hypovirus FgHV1 infection does not attenuate the virulence of the host (hypovirulence), it results in defects in mycelial growth and spore production. We now report that the vertical transmission rate of FgHV1 through asexual spores reached 100%. Using RNA deep sequencing, we performed genome-wide expression analysis to reveal phenotype-related genes with expression changes in response to FgHV1 infection. A total of 378 genes were differentially expressed, suggesting that hypovirus infection causes a significant alteration of fungal gene expression. Nearly two times as many genes were up-regulated as were down-regulated. A differentially expressed gene enrichment analysis identified a number of important pathways. Metabolic processes, the ubiquitination system, and especially cellular redox regulation were the most affected categories in F. graminearum challenged with FgHV1. The p20, encoded by FgHV1 could induce H₂O₂ accumulation and hypersensitive response in Nicotiana benthamiana leaves. Moreover, hypovirus FgHV1 may regulate transcription factors and trigger the RNA silencing pathway in F. graminearum. Full article

(This article belongs to the Special Issue Host-Microbe Interaction)

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11 pages, 729 KiB

Open AccessArticle

The Evolution of Total Phenolic Compounds and Antioxidant Activities during Ripening of Grapes (Vitis vinifera L., cv. Tempranillo) Grown in Semiarid Region: Effects of Cluster Thinning and Water Deficit

by Inmaculada Garrido¹, David Uriarte², Marcos Hernández³, José Luis Llerena^1,4

, María Esperanza Valdés⁵ and Francisco Espinosa^1,*

¹ Department of Plant Biology, Ecology and Earth Sciences, University of Extremadura, 06006 Badajoz, Spain

² CICYTEX-Institute of Agricultural Research Finca La Orden-Valdesequera, Ctra. A-V, Km 372, 06187 Badajoz, Spain

³ Aula Dei Scientific Technological Park Foundation, Av. Montañana 930, 50059 Zaragoza, Spain

⁴ Agri-Food Technological Center of Extremadura-CTAEX, 06195 Badajoz, Spain

⁵ CICYTEX-Technological Institute of Food and Agriculture-INTAEX, Av. Adolfo Suárez s/n, 06071 Badajoz, Spain

Int. J. Mol. Sci. 2016, 17(11), 1923; https://doi.org/10.3390/ijms17111923 - 17 Nov 2016

Cited by 25 | Viewed by 5072

Abstract

A study was made of how water status (rainfed vs. irrigated) and crop load (no cluster thinning vs. cluster thinning) can together affect the grapes of Vitis vinifera cv. Tempranillo vines growing in a semiarid zone of Extremadura (Spain). The grapes were monitored [...] Read more.

A study was made of how water status (rainfed vs. irrigated) and crop load (no cluster thinning vs. cluster thinning) can together affect the grapes of Vitis vinifera cv. Tempranillo vines growing in a semiarid zone of Extremadura (Spain). The grapes were monitored at different stages of ripening, measuring the peroxidase (POX) and superoxide dismutase (SOD) antioxidant activities and the phenolic content (flavonoids and phenylpropanoids), together with other parameters. The irrigation regime was adjusted to provide 100% of crop evapotranspiration (ETc). The findings confirmed previous results that both thinning and water deficit advance ripening, while irrigation and high crop load (no thinning) lengthen the growth cycle. The SOD activity remained practically constant throughout ripening in the thinned treatments and was always lower than in the unthinned treatments, an aspect which could have been the cause of the observed greater level of lipid peroxidation in the water deficit, thinned treatment. The nonspecific peroxidase activity was very low, especially in the thinned treatments. The effect of thinning was enhanced when combined with water deficit, inducing increases in phenylpropanoids and, above all, flavonoids at the harvest stage of ripening, while leaving the polyphenol oxidase activity (PPO) unaffected. Full article

(This article belongs to the Special Issue Bioactive Phytochemicals and Functional Food Ingredients in Fruits and Vegetables 2016)

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14 pages, 1957 KiB

Open AccessArticle

Varietal Dependence of GLVs Accumulation and LOX-HPL Pathway Gene Expression in Four Vitis vinifera Wine Grapes

by Xu Qian^1,†

, Xiao-Qing Xu^1,†, Ke-Ji Yu¹, Bao-Qing Zhu², Yi-Bin Lan¹, Chang-Qing Duan¹ and Qiu-Hong Pan^1,*

¹ Center for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

² Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China

^† These authors equally contributed to this work.

Int. J. Mol. Sci. 2016, 17(11), 1924; https://doi.org/10.3390/ijms17111924 - 23 Nov 2016

Cited by 39 | Viewed by 6740

Abstract

Variety is one of the major factors influencing grape and wine aromatic characteristics. Green leaf volatiles (GLVs), derived from lipoxygenase-hydroperoxides lyase (LOX-HPL) pathway, are important components for the aromatic quality of grapes and wines. However, the varietal difference regarding GLVs accumulation and related [...] Read more.

Variety is one of the major factors influencing grape and wine aromatic characteristics. Green leaf volatiles (GLVs), derived from lipoxygenase-hydroperoxides lyase (LOX-HPL) pathway, are important components for the aromatic quality of grapes and wines. However, the varietal difference regarding GLVs accumulation and related gene expression are poorly studied. This work exhibited that the accumulation of various GLVs and the expression of LOX-HPL pathway genes in four Vitis vinifera wine grape cultivars: Syrah, Muscat Tchervine, Gewürztraminer and Chardonnay. The results showed a variety dependence of GLVs profile. Muscat Tchervine harvested grapes contained less C6 aldehydes and the most abundant esters, which corresponded to very low VvLOXA and VvHPL1 expression abundance as well as high VvAAT transcript in this variety. High expression level of both VvLOXA and VvHPL1 paralleled with higher level of C6 aldehydes together with higher alcohols in Syrah grape. Gewürztraminer and Chardonnay grapes had high aldehydes and alcohols as well as low esters, which were resulted from their higher expression level of VvLOXA or VvHPL1 and lower VvAAT. From these above corresponding relations, it is concluded that VvLOXA, VvHPL1 and VvAAT in the LOX-HPL pathway are targets for altering GLVs composition in the grape varieties. Full article

(This article belongs to the Section Molecular Plant Sciences)

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14 pages, 7002 KiB

Open AccessArticle

3D Visualization of Developmental Toxicity of 2,4,6-Trinitrotoluene in Zebrafish Embryogenesis Using Light-Sheet Microscopy

by Juneyong Eum^1,†, Jina Kwak^1,†, Hee Joung Kim^2,†, Seoyoung Ki¹, Kooyeon Lee¹, Ahmed A. Raslan¹, Ok Kyu Park³, Md Ashraf Uddin Chowdhury¹, Song Her³, Yun Kee¹, Seung-Hae Kwon^3,* and Byung Joon Hwang^4,*

¹ Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea

² Division of Environmental Research Center, Kangwon National University, Chuncheon 24341, Korea

³ Korea Basic Science Institute, Chuncheon Center, Chuncheon 24341, Korea

⁴ Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Korea

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1925; https://doi.org/10.3390/ijms17111925 - 17 Nov 2016

Cited by 17 | Viewed by 8230

Abstract

Environmental contamination by trinitrotoluene is of global concern due to its widespread use in military ordnance and commercial explosives. Despite known long-term persistence in groundwater and soil, the toxicological profile of trinitrotoluene and other explosive wastes have not been systematically measured using in [...] Read more.

Environmental contamination by trinitrotoluene is of global concern due to its widespread use in military ordnance and commercial explosives. Despite known long-term persistence in groundwater and soil, the toxicological profile of trinitrotoluene and other explosive wastes have not been systematically measured using in vivo biological assays. Zebrafish embryos are ideal model vertebrates for high-throughput toxicity screening and live in vivo imaging due to their small size and transparency during embryogenesis. Here, we used Single Plane Illumination Microscopy (SPIM)/light sheet microscopy to assess the developmental toxicity of explosive-contaminated water in zebrafish embryos and report 2,4,6-trinitrotoluene-associated developmental abnormalities, including defects in heart formation and circulation, in 3D. Levels of apoptotic cell death were higher in the actively developing tissues of trinitrotoluene-treated embryos than controls. Live 3D imaging of heart tube development at cellular resolution by light-sheet microscopy revealed trinitrotoluene-associated cardiac toxicity, including hypoplastic heart chamber formation and cardiac looping defects, while the real time PCR (polymerase chain reaction) quantitatively measured the molecular changes in the heart and blood development supporting the developmental defects at the molecular level. Identification of cellular toxicity in zebrafish using the state-of-the-art 3D imaging system could form the basis of a sensitive biosensor for environmental contaminants and be further valued by combining it with molecular analysis. Full article

(This article belongs to the Special Issue Zebrafish: A Model for Toxicological Research)

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14 pages, 3002 KiB

Open AccessArticle

Nitrogen Removal from Landfill Leachate by Microalgae

by Sérgio F. L. Pereira^1,2, Ana L. Gonçalves¹, Francisca C. Moreira², Tânia F. C. V. Silva²

, Vítor J. P. Vilar² and José C. M. Pires^1,*

¹ Laboratório de Engenharia de Processos, Ambiente e Energia (LEPABE), Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal

² Laboratory of Separation and Reaction Engineering—Laboratory of Catalysis and Materials (LSRE-LCM), Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal

Int. J. Mol. Sci. 2016, 17(11), 1926; https://doi.org/10.3390/ijms17111926 - 17 Nov 2016

Cited by 48 | Viewed by 6849

Abstract

Landfill leachates result from the degradation of solid residues in sanitary landfills, thus presenting a high variability in terms of composition. Normally, these effluents are characterized by high ammoniacal-nitrogen (N–NH₄⁺) concentrations, high chemical oxygen demands and low phosphorus concentrations. The [...] Read more.

Landfill leachates result from the degradation of solid residues in sanitary landfills, thus presenting a high variability in terms of composition. Normally, these effluents are characterized by high ammoniacal-nitrogen (N–NH₄⁺) concentrations, high chemical oxygen demands and low phosphorus concentrations. The development of effective treatment strategies becomes difficult, posing a serious problem to the environment. Phycoremediation appears to be a suitable alternative for the treatment of landfill leachates. In this study, the potential of Chlorella vulgaris for biomass production and nutrients (mainly nitrogen and phosphorus) removal from different compositions of a landfill leachate was evaluated. Since microalgae also require phosphorus for their growth, different loads of this nutrient were evaluated, giving the following N:P ratios: 12:1, 23:1 and 35:1. The results have shown that C. vulgaris was able to grow in the different leachate compositions assessed. However, microalgal growth was higher in the cultures presenting the lowest N–NH₄⁺ concentration. In terms of nutrients uptake, an effective removal of N–NH₄⁺ and phosphorus was observed in all the experiments, especially in those supplied with phosphorus. Nevertheless, N–NO₃⁻ removal was considered almost negligible. These promising results constitute important findings in the development of a bioremediation technology for the treatment of landfill leachates. Full article

(This article belongs to the Special Issue Algae Based Bio-Renewable Energy for Sustainability)

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13 pages, 5802 KiB

Open AccessArticle

Pterostilbene Inhibits Human Multiple Myeloma Cells via ERK1/2 and JNK Pathway In Vitro and In Vivo

by Bingqian Xie^1,†, Zhijian Xu^2,†, Liangning Hu^1,†, Gege Chen¹, Rong Wei¹, Guang Yang¹, Bo Li², Gaomei Chang¹, Xi Sun¹, Huiqun Wu¹, Yong Zhang², Bojie Dai³, Yi Tao^1,*, Jumei Shi^1,* and Weiliang Zhu^2,*

¹ Department of Hematology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China

² CAS Key Laboratory of Receptor Research, Drug Discovery and Design Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

³ College of Life Science and Technology, Tongji University, Shanghai 200092, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1927; https://doi.org/10.3390/ijms17111927 - 17 Nov 2016

Cited by 41 | Viewed by 8128

Abstract

Multiple myeloma (MM) is the second most common malignancy in the hematologic system, which is characterized by accumulation of plasma cells in bone marrow. Pterostilbene (PTE) is a natural dimethylated analog of resveratrol, which has anti-oxidant, anti-inflammatory and anti-tumor properties. In the present [...] Read more.

Multiple myeloma (MM) is the second most common malignancy in the hematologic system, which is characterized by accumulation of plasma cells in bone marrow. Pterostilbene (PTE) is a natural dimethylated analog of resveratrol, which has anti-oxidant, anti-inflammatory and anti-tumor properties. In the present study, we examined the anti-tumor effect of PTE on MM cell lines both in vitro and in vivo using the cell counting kit (CCK)-8, apoptosis assays, cell cycle analysis, reactive oxygen species (ROS) generation, JC-1 mitochondrial membrane potential assay, Western blotting and tumor xenograft models. The results demonstrated that PTE induces apoptosis in the H929 cell line and causes cell cycle arrest at G0/G1 phase by enhancing ROS generation and reducing mitochondrial membrane potential. The anti-tumor effect of PTE may be caused by the activation of the extracellular regulated protein kinases (ERK) 1/2 and c-Jun N-terminal kinase (JNK) signaling pathways. Additionally, mice treated with PTE by intraperitoneal injection demonstrated reduced tumor volume. Taken together, the results of this study indicate that the anti-tumor effect of PTE on MM cells may provide a new therapeutic option for MM patients. Full article

(This article belongs to the Section Biochemistry)

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15 pages, 3499 KiB

Open AccessArticle

Toosendanin Exerts an Anti-Cancer Effect in Glioblastoma by Inducing Estrogen Receptor β- and p53-Mediated Apoptosis

by Liang Cao^1,†, Dingding Qu^2,†, Huan Wang^2,†, Sha Zhang¹, Chenming Jia¹, Zixuan Shi³, Zongren Wang¹, Jian Zhang^2,* and Jing Ma^1,*

¹ Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

² Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi’an 710032, China

³ Department of Acupuncture, Shaanxi Hospital of Traditional Chinese Medicine, Xi’an 710032, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1928; https://doi.org/10.3390/ijms17111928 - 18 Nov 2016

Cited by 44 | Viewed by 6255

Abstract

Glioblastoma (GBM) is the most common primary brain tumor with median survival of approximately one year. This dismal poor prognosis is due to resistance to currently available chemotherapeutics; therefore, new cytotoxic agents are urgently needed. In the present study, we reported the cytotoxicity [...] Read more.

Glioblastoma (GBM) is the most common primary brain tumor with median survival of approximately one year. This dismal poor prognosis is due to resistance to currently available chemotherapeutics; therefore, new cytotoxic agents are urgently needed. In the present study, we reported the cytotoxicity of toosendanin (TSN) in the GBM U87 and C6 cell lines in vitro and in vivo. By using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay, flow cytometry analysis, and Western blot, we found that TSN inhibited U87 and C6 cell proliferation and induced apoptosis at a concentration as low as 10 nM. Administration of TSN also reduced tumor burden in a xenograft model of athymic nude mice. Pharmacological and molecular studies suggested that estrogen receptor β (ERβ) and p53 were prominent targets for TSN. GBM cell apoptosis induced by TSN was a stepwise biological event involving the upregulation of ERβ and contextual activation of functional p53. Collectively, our study indicates, for the first time, that TSN is a candidate of novel anti-cancer drugs for GBM. Furthermore, ERβ and p53 could act as predictive biomarkers for the sensitivity of cancer to TSN. Full article

(This article belongs to the Special Issue Translational Molecular Medicine & Molecular Drug Discovery)

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15 pages, 2827 KiB

Open AccessReview

Functional Role of the C-Terminal Amphipathic Helix 8 of Olfactory Receptors and Other G Protein-Coupled Receptors

by Takaaki Sato^1,*, Takashi Kawasaki¹, Shouhei Mine¹ and Hiroyoshi Matsumura²

¹ Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorioka, Ikeda, Osaka 563-8577, Japan

² College of Life Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan

Int. J. Mol. Sci. 2016, 17(11), 1930; https://doi.org/10.3390/ijms17111930 - 18 Nov 2016

Cited by 30 | Viewed by 8379

Abstract

G protein-coupled receptors (GPCRs) transduce various extracellular signals, such as neurotransmitters, hormones, light, and odorous chemicals, into intracellular signals via G protein activation during neurological, cardiovascular, sensory and reproductive signaling. Common and unique features of interactions between GPCRs and specific G proteins are [...] Read more.

G protein-coupled receptors (GPCRs) transduce various extracellular signals, such as neurotransmitters, hormones, light, and odorous chemicals, into intracellular signals via G protein activation during neurological, cardiovascular, sensory and reproductive signaling. Common and unique features of interactions between GPCRs and specific G proteins are important for structure-based design of drugs in order to treat GPCR-related diseases. Atomic resolution structures of GPCR complexes with G proteins have revealed shared and extensive interactions between the conserved DRY motif and other residues in transmembrane domains 3 (TM3), 5 and 6, and the target G protein C-terminal region. However, the initial interactions formed between GPCRs and their specific G proteins remain unclear. Alanine scanning mutagenesis of the murine olfactory receptor S6 (mOR-S6) indicated that the N-terminal acidic residue of helix 8 of mOR-S6 is responsible for initial transient and specific interactions with chimeric Gα_{15_olf}, resulting in a response that is 2.2-fold more rapid and 1.7-fold more robust than the interaction with Gα₁₅. Our mutagenesis analysis indicates that the hydrophobic core buried between helix 8 and TM1–2 of mOR-S6 is important for the activation of both Gα_{15_olf} and Gα₁₅. This review focuses on the functional role of the C-terminal amphipathic helix 8 based on several recent GPCR studies. Full article

(This article belongs to the Collection G Protein-Coupled Receptor Signaling and Regulation)

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13 pages, 1751 KiB

Open AccessArticle

Protective Effect of Tempol against Cisplatin-Induced Ototoxicity

by Cha Kyung Youn^1,2,†, Jun Kim^1,†, Eu-Ri Jo¹, Jeonghyun Oh¹, Nam Yong Do¹ and Sung Il Cho^1,*

¹ Department of Otolaryngology-Head and Neck Surgery, Chosun University School of Medicine, Gwangju 61453, Korea

² Division of Natural Medical Science, Chosun University School of Medicine, Gwangju 61452, Korea

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1931; https://doi.org/10.3390/ijms17111931 - 18 Nov 2016

Cited by 20 | Viewed by 6676

Abstract

One of the major adverse effects of cisplatin chemotherapy is hearing loss. Cisplatin-induced ototoxicity hampers treatment because it often necessitates dose reduction, which decreases cisplatin efficacy. This study was performed to investigate the effect of Tempol on cisplatin-induced ototoxicity in an auditory cell [...] Read more.

One of the major adverse effects of cisplatin chemotherapy is hearing loss. Cisplatin-induced ototoxicity hampers treatment because it often necessitates dose reduction, which decreases cisplatin efficacy. This study was performed to investigate the effect of Tempol on cisplatin-induced ototoxicity in an auditory cell line, House Ear Institute-Organ of Corti 1 (HEI-OC1). Cultured HEI-OC1 cells were exposed to 30 μM cisplatin for 24 h with or without a 2 h pre-treatment with Tempol. Cell viability was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and apoptotic cells were identified using terminal deoxynucleotidyl transferase dUTP nick end labeling of nuclei (TUNEL) assay and flow cytometry. The effects of Tempol on cisplatin-induced cleaved poly(ADP-ribose) polymerase, cleaved caspase, and mitochondrial inducible nitric oxide synthase expression were evaluated using western blot analysis. Levels of intracellular reactive oxygen species (ROS) were measured to assess the effects of Tempol on cisplatin-induced ROS accumulation. Mitochondria were evaluated by confocal microscopy, and the mitochondrial membrane potential was measured to investigate whether Tempol protected against cisplatin-induced mitochondrial dysfunction. Cisplatin treatment decreased cell viability, and increased apoptotic features and markers, ROS accumulation, and mitochondrial dysfunction. Tempol pre-treatment before cisplatin exposure significantly inhibited all these cisplatin-induced effects. These results demonstrate that Tempol inhibits cisplatin-induced cytotoxicity in HEI-OC1, and could play a preventive role against cisplatin-induced ototoxicity. Full article

(This article belongs to the Special Issue Neuroprotective Strategies 2016)

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14 pages, 4262 KiB

Open AccessArticle

Isoliquiritigenin Attenuates Atherogenesis in Apolipoprotein E-Deficient Mice

by Fen Du^1,†, Quzhen Gesang^1,2,†, Jia Cao¹, Mei Qian¹, Li Ma¹, Dongfang Wu³ and Hong Yu^1,*

¹ Department of Biochemistry and Molecular Biology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University School of Basic Medical Sciences, 185 Donghu Road, Bldg. 2, 2-209, Wuhan 430071, China

² Medical College, Tibet University, Lhasa 850000, China

³ Department of Pharmacy, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1932; https://doi.org/10.3390/ijms17111932 - 18 Nov 2016

Cited by 29 | Viewed by 6798

Abstract

Isoliquiritigenin (ISL) exhibits antioxidation and anti-inflammation activity. We sought to investigate the effects and mechanism of ISL on the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE^−/−) mice. Firstly, we determined that ISL reduced the mRNA levels of inflammatory factors interleukin [...] Read more.

Isoliquiritigenin (ISL) exhibits antioxidation and anti-inflammation activity. We sought to investigate the effects and mechanism of ISL on the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE^−/−) mice. Firstly, we determined that ISL reduced the mRNA levels of inflammatory factors interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and monocyte chemotactic protein-1 (MCP-1), while it increased the expression of several lipoprotein-related genes in peritoneal macrophages treated with lipopolysaccharide (LPS). ISL also enhanced peroxisome proliferator-activated receptor gamma (PPARγ) protein levels and reversed the changes of ATP-binding cassette transporter A (ABCA1) and cluster of differentiation 36 (CD36) in macrophages treated with oxidative low-density lipoprotein (ox-LDL). Then, in an in vivo study, female apoE^−/− mice were fed a Western diet with ISL (0, 20, 100 mg/kg/day) added for 12 weeks. We found that ISL decreased the plasma cholesterol levels of very low-density lipoprotein (VLDL)/LDL, promoted plasma superoxide dismutase (SOD) and paraoxonase-1 (PON1) activities, and decreased plasma IL-6, TNF-α, and MCP-1 levels. Moreover, ISL significantly reduced the atherosclerotic lesions and hepatic steatosis in apoE^−/− mice. In the liver, ISL altered the expression of several key genes (such as SRBI, ABCA1, ABCG8, PPARγ, and FASN) involving cholesterol-selective uptake and excretion into bile, triglyceride (TG) biosynthesis, and inflammation. These results suggest that the atheroprotective effects of ISL are due to the improvement of lipid metabolism, antioxidation, and anti-inflammation, which involve PPARγ-dependent signaling. Full article

(This article belongs to the Special Issue Vascular Biology and Therapeutics)

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17 pages, 18641 KiB

Open AccessArticle

Identification and Expression Analysis of Polygalacturonase Family Members during Peach Fruit Softening

by Ming Qian

, Yike Zhang, Xiangyan Yan, Mingyu Han, Jinjin Li, Fang Li, Furui Li, Dong Zhang and Caiping Zhao^*

College of Horticulture, Northwest A&F University, Yangling 712100, China

Int. J. Mol. Sci. 2016, 17(11), 1933; https://doi.org/10.3390/ijms17111933 - 18 Nov 2016

Cited by 59 | Viewed by 6979

Abstract

Polygalacturonase (PG) is an important hydrolytic enzyme involved in pectin degradation during fruit softening. However, the roles of PG family members in fruit softening remain unclear. We identified 45 PpPG genes in the peach genome which are clustered into six subclasses. PpPGs consist [...] Read more.

Polygalacturonase (PG) is an important hydrolytic enzyme involved in pectin degradation during fruit softening. However, the roles of PG family members in fruit softening remain unclear. We identified 45 PpPG genes in the peach genome which are clustered into six subclasses. PpPGs consist of four to nine exons and three to eight introns, and the exon/intron structure is basically conserved in all but subclass E. Only 16 PpPG genes were expressed in ripening fruit, and their expression profiles were analyzed during storage in two peach cultivars with different softening characteristics. Eight PGs (PpPG1, -10, -12, -13, -15, -23, -21, and -22) in fast-softening “Qian Jian Bai” (QJB) fruit and three PGs (PpPG15, -21, and -22) in slow-softening “Qin Wang” (QW) fruit exhibited softening-associated patterns; which also were affected by ethylene treatment. Our results suggest that the different softening characters in QW and QJB fruit is related to the amount of PG members. While keeping relatively lower levels during QW fruit softening, the expression of six PGs (PpPG1, -10, -12, -11, -14, and -35) rapidly induced by ethylene. PpPG24, -25 and -38 may not be involved in softening of peach fruit. Full article

(This article belongs to the Special Issue Ripening Control and Induction of the Defence and Antioxidant Systems)

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20 pages, 3654 KiB

Open AccessArticle

Design of New Antibacterial Enhancers Based on AcrB’s Structure and the Evaluation of Their Antibacterial Enhancement Activity

by Yi Song^1,†, Rongxin Qin^1,†, Xichun Pan¹, Qin Ouyang²

, Tianyu Liu², Zhaoxia Zhai¹, Yingchun Chen², Bin Li¹ and Hong Zhou^1,*

¹ Department of Pharmacology, College of Pharmacy, The Third Military Medical University, Chongqing 400038, China

² Department of Medicinal Chemistry, College of Pharmacy, The Third Military Medical University, Chongqing 400038, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1934; https://doi.org/10.3390/ijms17111934 - 18 Nov 2016

Cited by 14 | Viewed by 5069

Abstract

Previously, artesunate (AS) and dihydroartemisinine 7 (DHA7) were found to have antibacterial enhancement activity against Escherichia coli via inhibition of the efflux pump AcrB. However, they were only effective against E. coli standard strains. This study aimed to develop effective antibacterial enhancers based [...] Read more.

Previously, artesunate (AS) and dihydroartemisinine 7 (DHA7) were found to have antibacterial enhancement activity against Escherichia coli via inhibition of the efflux pump AcrB. However, they were only effective against E. coli standard strains. This study aimed to develop effective antibacterial enhancers based on the previous work. Our results demonstrate that 86 new antibacterial enhancers were designed via 3D-SAR and molecular docking. Among them, DHA27 had the best antibacterial enhancement activity. It could potentiate the antibacterial effects of ampicillin against not only E. coli standard strain but also clinical strains, and of β-lactam antibiotics, not non-β-lactamantibiotics. DHA27 could increase the accumulation of daunomycin and nile red within E. coli ATCC 35218, but did not increase the bacterial membrane permeability. DHA27 reduced acrB’s mRNA expression of E. coli ATCC 35218 in a dose-dependent manner, and its antibacterial enhancement activity is related to the degree of acrB mRNA expression in E. coli clinical strains. The polypeptides from AcrB were obtained via molecular docking assay; the pre-incubated polypeptides could inhibit the activity of DHA27. Importantly, DHA27 had no cytotoxicity on cell proliferation. In conclusion, among newly designed antibacterial enhancers, DHA27 had favorable physical and pharmacological properties with no significant cytotoxicity at effective concentrations, and might serve as a potential efflux pump inhibitor in the future. Full article

(This article belongs to the Section Bioactives and Nutraceuticals)

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8 pages, 354 KiB

Open AccessArticle

Clinical Decision-Making in the Treatment of Schizophrenia: Focus on Long-Acting Injectable Antipsychotics

by Ludovic Samalin^1,2,3, Marion Garnier^1,3, Candy Auclair⁴ and Pierre-Michel Llorca^1,2,3,*

¹ Centre Hospitalier et Universitaire, Department of Psychiatry, University of Auvergne, EA 7280, 63000 Clermont-Ferrand, France

² French Society for Biological Psychiatry and Neuropsychopharmacology, CH Sainte Anne, 75674 Paris, France

³ Fondation FondaMental, Hôpital Albert Chenevier, Depatment of Psychiatry, 94000 Créteil, France

⁴ Centre Hospitalier et Universitaire, Department of Public Health, University of Auvergne, EA 4681 PEPRADE, 63000 Clermont-Ferrand, France

Int. J. Mol. Sci. 2016, 17(11), 1935; https://doi.org/10.3390/ijms17111935 - 19 Nov 2016

Cited by 19 | Viewed by 7937

Abstract

The purpose of this study was to identify clinician characteristics associated with higher prescription rates of long-acting injectable (LAI) antipsychotics, as well as the sources that influence medical decision-making regarding the treatment of schizophrenia. We surveyed 202 psychiatrists during six regional French conferences [...] Read more.

The purpose of this study was to identify clinician characteristics associated with higher prescription rates of long-acting injectable (LAI) antipsychotics, as well as the sources that influence medical decision-making regarding the treatment of schizophrenia. We surveyed 202 psychiatrists during six regional French conferences (Bordeaux, Lyon, Marseille, Nice, Paris, and Strasbourg). Data on the characteristics of practice, prescription rates of antipsychotic, and information sources about their clinical decisions were collected. Most psychiatrists used second-generation antipsychotics (SGAs), and preferentially an oral formulation, in the treatment of schizophrenia. LAI SGAs were prescribed to 30.4% of schizophrenic patients. The duration and type of practice did not influence the class or formulation of antipsychotics used. The clinicians following the higher percentage of schizophrenic patients were associated with a higher use of LAI antipsychotics and a lower use of oral SGAs. Personal experience, government regulatory approval, and guidelines for the treatment of schizophrenia were the three main contributing factors guiding clinicians’ decision-making regarding the treatment of schizophrenia. The more clinicians follow schizophrenic patients, the more they use LAI antipsychotics. The development of specialized programs with top specialists should lead to better use of LAI antipsychotics in the treatment of schizophrenia. Full article

(This article belongs to the Special Issue Antipsychotics)

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12 pages, 5396 KiB

Open AccessArticle

Synthesis of Non-Toxic Silica Particles Stabilized by Molecular Complex Oleic-Acid/Sodium Oleate

by Catalin Ilie Spataru, Raluca Ianchis, Cristian Petcu, Cristina Lavinia Nistor^*

, Violeta Purcar, Bogdan Trica

, Sabina Georgiana Nitu, Raluca Somoghi, Elvira Alexandrescu, Florin Oancea

and Dan Donescu

National Research & Development Institute for Chemistry and Petrochemistry—ICECHIM, Spl. Independentei 202, 6th District, Bucharest 060021, Romania

Int. J. Mol. Sci. 2016, 17(11), 1936; https://doi.org/10.3390/ijms17111936 - 19 Nov 2016

Cited by 11 | Viewed by 7731

Abstract

The present work is focused on the preparation of biocompatible silica particles from sodium silicate, stabilized by a vesicular system containing oleic acid (OLA) and its alkaline salt (OLANa). Silica nanoparticles were generated by the partial neutralization of oleic acid (OLA), with the [...] Read more.

The present work is focused on the preparation of biocompatible silica particles from sodium silicate, stabilized by a vesicular system containing oleic acid (OLA) and its alkaline salt (OLANa). Silica nanoparticles were generated by the partial neutralization of oleic acid (OLA), with the sodium cation present in the aqueous solutions of sodium silicate. At the molar ratio OLA/Na⁺ = 2:1, the molar ratio (OLA/OLANa = 1:1) required to form vesicles, in which the carboxyl and carboxylate groups have equal concentrations, was achieved. In order to obtain hydrophobically modified silica particles, octadecyltriethoxysilane (ODTES) was added in a sodium silicate sol–gel mixture at different molar ratios. The interactions between the octadecyl groups from the modified silica and the oleyl chains from the OLA/OLANa stabilizing system were investigated via simultaneous thermogravimetry (TG) and differential scanning calorimetry (DSC) (TG-DSC) analyses.A significant decrease in vaporization enthalpy and an increase in amount of ODTES were observed. Additionally, that the hydrophobic interaction between OLA and ODTES has a strong impact on the hybrids’ final morphology and on their textural characteristics was revealed. The highest hydrodynamic average diameter and the most negative ζ potential were recorded for the hybrid in which the ODTES/sodium silicate molar ratio was 1:5. The obtained mesoporous silica particles, stabilized by the OLA/OLANa vesicular system, may find application as carriers for hydrophobic bioactive molecules. Full article

(This article belongs to the Special Issue Bioactive Nanoparticles)

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10 pages, 1471 KiB

Open AccessArticle

The Vitamin E Analog Gamma-Tocotrienol (GT3) and Statins Synergistically Up-Regulate Endothelial Thrombomodulin (TM)

by Rupak Pathak^1,*

, Sanchita P. Ghosh², Daohong Zhou¹ and Martin Hauer-Jensen^1,3

¹ Division of Radiation Health, College of Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA

² Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20889, USA

³ Surgical Service, Central Arkansas Veterans Healthcare System, Little Rock, AR 72205, USA

Int. J. Mol. Sci. 2016, 17(11), 1937; https://doi.org/10.3390/ijms17111937 - 18 Nov 2016

Cited by 13 | Viewed by 5418

Abstract

Statins; a class of routinely prescribed cholesterol-lowering drugs; inhibit 3-hydroxy-3-methylglutaryl-coenzymeA reductase (HMGCR) and strongly induce endothelial thrombomodulin (TM); which is known to have anti-inflammatory; anti-coagulation; anti-oxidant; and radioprotective properties. However; high-dose toxicity limits the clinical use of statins. The vitamin E family member [...] Read more.

Statins; a class of routinely prescribed cholesterol-lowering drugs; inhibit 3-hydroxy-3-methylglutaryl-coenzymeA reductase (HMGCR) and strongly induce endothelial thrombomodulin (TM); which is known to have anti-inflammatory; anti-coagulation; anti-oxidant; and radioprotective properties. However; high-dose toxicity limits the clinical use of statins. The vitamin E family member gamma-tocotrienol (GT3) also suppresses HMGCR activity and induces TM expression without causing significant adverse side effects; even at high concentrations. To investigate the synergistic effect of statins and GT3 on TM; a low dose of atorvastatin and GT3 was used to treat human primary endothelial cells. Protein-level TM expression was measured by flow cytometry. TM functional activity was determined by activated protein C (APC) generation assay. Expression of Kruppel-like factor 2 (KLF2), one of the key transcription factors of TM, was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). TM expression increased in a dose-dependent manner after both atorvastatin and GT3 treatment. A combined treatment of a low-dose of atorvastatin and GT3 synergistically up-regulated TM expression and functional activity. Finally; atorvastatin and GT3 synergistically increased KLF2 expression. These findings suggest that combined treatment of statins with GT3 may provide significant health benefits in treating a number of pathophysiological conditions; including inflammatory and cardiovascular diseases. Full article

(This article belongs to the Special Issue Tocopherols and Tocotrienols: Metabolism and Properties)

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14 pages, 1495 KiB

Open AccessArticle

Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells

by Xueqin Li^1,†, Jun Shen^2,†, Yunyao Jiang^3,4,†, Ting Shen^5,8, Long You^5,8, Xiaobo Sun⁶, Xudong Xu⁶, Weicheng Hu^5,8,*

, Haifeng Wu^6,* and Gongcheng Wang^7,*

¹ Department of Gerontology, Huai’an First People’s Hospital, Nanjing Medical University, Huaian 223300, China

² Department of Neurology, Huai’an Hospital Affiliated of Xuzhou Medical College and Huai’an Second People’s Hospital, Huaian 223300, China

³ Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China

⁴ Shineway Pharmaceutical Group Co., Ltd., Shijiazhuang 051430, China

⁵ Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huaian 223300, China

⁶ Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China

⁷ Department of Urology, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing West Road, Huaian 223300, China

⁸ Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1938; https://doi.org/10.3390/ijms17111938 - 22 Nov 2016

Cited by 35 | Viewed by 7912

Abstract

Chloranthalactone B (CTB), a lindenane-type sesquiterpenoid, was obtained from the Chinese medicinal herb Sarcandra glabra, which is frequently used as a remedy for inflammatory diseases. However, the anti-inflammatory mechanisms of CTB have not been fully elucidated. In this study, we investigated the [...] Read more.

Chloranthalactone B (CTB), a lindenane-type sesquiterpenoid, was obtained from the Chinese medicinal herb Sarcandra glabra, which is frequently used as a remedy for inflammatory diseases. However, the anti-inflammatory mechanisms of CTB have not been fully elucidated. In this study, we investigated the molecular mechanisms underlying these effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. CTB strongly inhibited the production of nitric oxide and pro-inflammatory mediators such as prostaglandin E₂, tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 in RAW264.7 cells stimulated with LPS. A reverse-transcription polymerase chain reaction assay and Western blot further confirmed that CTB inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, and IL-1β at the transcriptional level, and decreased the luciferase activities of activator protein (AP)-1 reporter promoters. These data suggest that inhibition occurred at the transcriptional level. In addition, CTB blocked the activation of p38 mitogen-activated protein kinase (MAPK) but not c-Jun N-terminal kinase or extracellular signal-regulated kinase 1/2. Furthermore, CTB suppressed the phosphorylation of MKK3/6 by targeting the binding sites via formation of hydrogen bonds. Our findings clearly show that CTB inhibits the production of inflammatory mediators by inhibiting the AP-1 and p38 MAPK pathways. Therefore, CTB could potentially be used as an anti-inflammatory agent. Full article

(This article belongs to the Section Bioactives and Nutraceuticals)

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16 pages, 3317 KiB

Open AccessArticle

The Role of Deoxycytidine Kinase (dCK) in Radiation-Induced Cell Death

by Rui Zhong¹, Rui Xin^1,2, Zongyan Chen^1,3, Nan Liang¹, Yang Liu¹, Shumei Ma^1,* and Xiaodong Liu^1,4,*

¹ Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun 130021, China

² Department Radiology, the 2st Hospitals Affiliated to Jilin University, Changchun 130021, China

³ Department Diagnostic Imaging, Weihai Chest Hospital, Weihai 264220, China

⁴ Department Radiation Oncology, the 2nd Hospitals Affiliated to Jilin University, Changchun 130021, China

Int. J. Mol. Sci. 2016, 17(11), 1939; https://doi.org/10.3390/ijms17111939 - 21 Nov 2016

Cited by 11 | Viewed by 7024

Abstract

Deoxycytidine kinase (dCK) is a key enzyme in deoxyribonucleoside salvage and the anti-tumor activity for many nucleoside analogs. dCK is activated in response to ionizing radiation (IR)-induced DNA damage and it is phosphorylated on Serine 74 by the Ataxia-Telangiectasia Mutated (ATM) kinase in [...] Read more.

Deoxycytidine kinase (dCK) is a key enzyme in deoxyribonucleoside salvage and the anti-tumor activity for many nucleoside analogs. dCK is activated in response to ionizing radiation (IR)-induced DNA damage and it is phosphorylated on Serine 74 by the Ataxia-Telangiectasia Mutated (ATM) kinase in order to activate the cell cycle G2/M checkpoint. However, whether dCK plays a role in radiation-induced cell death is less clear. In this study, we genetically modified dCK expression by knocking down or expressing a WT (wild-type), S74A (abrogates phosphorylation) and S74E (mimics phosphorylation) of dCK. We found that dCK could decrease IR-induced total cell death and apoptosis. Moreover, dCK increased IR-induced autophagy and dCK-S74 is required for it. Western blotting showed that the ratio of phospho-Akt/Akt, phospho-mTOR/mTOR, phospho-P70S6K/P70S6K significantly decreased in dCK-WT and dCK-S74E cells than that in dCK-S74A cells following IR treatment. Reciprocal experiment by co-immunoprecipitation showed that mTOR can interact with wild-type dCK. IR increased polyploidy and decreased G2/M arrest in dCK knock-down cells as compared with control cells. Taken together, phosphorylated and activated dCK can inhibit IR-induced cell death including apoptosis and mitotic catastrophe, and promote IR-induced autophagy through PI3K/Akt/mTOR pathway. Full article

(This article belongs to the Collection Programmed Cell Death and Apoptosis)

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15 pages, 4814 KiB

Open AccessArticle

Hydrostatin-TL1, an Anti-Inflammatory Active Peptide from the Venom Gland of Hydrophis cyanocinctus in the South China Sea

by Ningyuan Wang^1,†, Yan Huang^2,†

, An Li^2,†, Hailong Jiang², Jie Wang², Jianzhong Li², Lei Qiu², Ka Li^3,* and Yiming Lu^2,*

¹ School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China

² Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

³ Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai 200032, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1940; https://doi.org/10.3390/ijms17111940 - 22 Nov 2016

Cited by 24 | Viewed by 6025

Abstract

Tumor necrosis factor (TNF)-α is a pleiotropic cytokine with intense pro-inflammatory and immunomodulatory properties, and anti-TNF-α biologics are effective therapies for various inflammatory diseases such as inflammatory bowel disease (IBD) and sepsis. Snake venom, as a traditional Chinese medicine, has been used in [...] Read more.

Tumor necrosis factor (TNF)-α is a pleiotropic cytokine with intense pro-inflammatory and immunomodulatory properties, and anti-TNF-α biologics are effective therapies for various inflammatory diseases such as inflammatory bowel disease (IBD) and sepsis. Snake venom, as a traditional Chinese medicine, has been used in the treatment of inflammatory diseases in China for centuries. In this research, we constructed a venom gland T7 phage display library of the sea snake Hydrophis cyanocinctus to screen bioactive compounds that antagonize TNF-α and identified a novel nine-amino-acid peptide, termed hydrostatin-TL1 (H-TL1). In enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) analyses, H-TL1 inhibited the interaction between TNF-α and TNF receptor 1 (TNFR1). Further, H-TL1 attenuated the cytotoxicity of TNF-α in L929 cells as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. H-TL1 also decreased the mRNA expression of TNF-α/TNFR1 downstream targets and suppressed the phosphorylation of well-characterized proteins of downstream signal transduction pathways in HEK-293 cells. In vivo data demonstrated that H-TL1 protects animals against dextran sodium sulfate (DSS)-induced acute colitis and lipopolysaccharide (LPS)-induced acute shock. Given its significant anti-inflammatory activity in vitro and in vivo, H-TL1 is a potential peptide for the development of new agents to treat TNF-α-associated inflammatory diseases. Full article

(This article belongs to the Section Biochemistry)

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22 pages, 1102 KiB

Open AccessReview

Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function

by Magda Dubińska-Magiera¹

, Małgorzata Daczewska^1,*, Anna Lewicka¹, Marta Migocka-Patrzałek¹

, Joanna Niedbalska-Tarnowska¹ and Krzysztof Jagla^2,*

¹ Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, 21 Sienkiewicza Street, 50-335 Wroclaw, Poland

² GReD—Genetics, Reproduction and Development Laboratory, INSERM U1103, CNRS UMR6293, University of Clermont-Auvergne, 28 Place Henri-Dunant, 63000 Clermont-Ferrand, France

Int. J. Mol. Sci. 2016, 17(11), 1941; https://doi.org/10.3390/ijms17111941 - 19 Nov 2016

Cited by 66 | Viewed by 13991

Abstract

The rapid progress in medicine, agriculture, and allied sciences has enabled the development of a large amount of potentially useful bioactive compounds, such as drugs and pesticides. However, there is another side of this phenomenon, which includes side effects and environmental pollution. To [...] Read more.

The rapid progress in medicine, agriculture, and allied sciences has enabled the development of a large amount of potentially useful bioactive compounds, such as drugs and pesticides. However, there is another side of this phenomenon, which includes side effects and environmental pollution. To avoid or minimize the uncontrollable consequences of using the newly developed compounds, researchers seek a quick and effective means of their evaluation. In achieving this goal, the zebrafish (Danio rerio) has proven to be a highly useful tool, mostly because of its fast growth and development, as well as the ability to absorb the molecules diluted in water through its skin and gills. In this review, we focus on the reports concerning the application of zebrafish as a model for assessing the impact of toxicants on skeletal muscles, which share many structural and functional similarities among vertebrates, including zebrafish and humans. Full article

(This article belongs to the Special Issue Zebrafish: A Model for Toxicological Research)

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16 pages, 1115 KiB

Open AccessReview

Immunomodulatory Function of the Tumor Suppressor p53 in Host Immune Response and the Tumor Microenvironment

by Yan Cui^* and Gang Guo

Department of Biochemistry and Molecular Biology, Cancer Immunology, Inflammation & Tolerance Program, Georgia Cancer Center, Augusta University, Augusta, GA 30912, USA

Int. J. Mol. Sci. 2016, 17(11), 1942; https://doi.org/10.3390/ijms17111942 - 19 Nov 2016

Cited by 109 | Viewed by 21495

Abstract

The tumor suppressor p53 is the most frequently mutated gene in human cancers. Most of the mutations are missense leading to loss of p53 function in inducing apoptosis and senescence. In addition to these autonomous effects of p53 inactivation/dysfunction on tumorigenesis, compelling evidence [...] Read more.

The tumor suppressor p53 is the most frequently mutated gene in human cancers. Most of the mutations are missense leading to loss of p53 function in inducing apoptosis and senescence. In addition to these autonomous effects of p53 inactivation/dysfunction on tumorigenesis, compelling evidence suggests that p53 mutation/inactivation also leads to gain-of-function or activation of non-autonomous pathways, which either directly or indirectly promote tumorigenesis. Experimental and clinical results suggest that p53 dysfunction fuels pro-tumor inflammation and serves as an immunological gain-of-function driver of tumorigenesis via skewing immune landscape of the tumor microenvironment (TME). It is now increasingly appreciated that p53 dysfunction in various cellular compartments of the TME leads to immunosuppression and immune evasion. Although our understanding of the cellular and molecular processes that link p53 activity to host immune regulation is still incomplete, it is clear that activating/reactivating the p53 pathway in the TME also represents a compelling immunological strategy to reverse immunosuppression and enhance antitumor immunity. Here, we review our current understanding of the potential cellular and molecular mechanisms by which p53 participates in immune regulation and discuss how targeting the p53 pathway can be exploited to alter the immunological landscape of tumors for maximizing therapeutic outcome. Full article

(This article belongs to the Special Issue Emerging Non-Canonical Functions and Regulation of p53)

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20 pages, 4989 KiB

Open AccessArticle

Early Antipsychotic Treatment in Juvenile Rats Elicits Long-Term Alterations to the Dopamine Neurotransmitter System

by Michael De Santis^1,2

, Jiamei Lian^1,2, Xu-Feng Huang² and Chao Deng^1,2,*

¹ Antipsychotic Research Laboratory, Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia

² School of Medicine, University of Wollongong, Wollongong, NSW 2522, Australia

Int. J. Mol. Sci. 2016, 17(11), 1944; https://doi.org/10.3390/ijms17111944 - 22 Nov 2016

Cited by 13 | Viewed by 6300

Abstract

Prescription of antipsychotic drugs (APDs) to children has substantially increased in recent years. Whilst current investigations into potential long-term effects have uncovered some alterations to adult behaviours, further investigations into potential changes to neurotransmitter systems are required. The current study investigated potential long-term [...] Read more.

Prescription of antipsychotic drugs (APDs) to children has substantially increased in recent years. Whilst current investigations into potential long-term effects have uncovered some alterations to adult behaviours, further investigations into potential changes to neurotransmitter systems are required. The current study investigated potential long-term changes to the adult dopamine (DA) system following aripiprazole, olanzapine and risperidone treatment in female and male juvenile rats. Levels of tyrosine hydroxylase (TH), phosphorylated-TH (p-TH), dopamine active transporter (DAT), and D₁ and D₂ receptors were measured via Western blot and/or receptor autoradiography. Aripiprazole decreased TH and D₁ receptor levels in the ventral tegmental area (VTA) and p-TH levels in the prefrontal cortex (PFC) of females, whilst TH levels decreased in the PFC of males. Olanzapine decreased PFC p-TH levels and increased D₂ receptor expression in the PFC and nucleus accumbens (NAc) in females only. Additionally, risperidone treatment increased D₁ receptor levels in the hippocampus of females, whilst, in males, p-TH levels increased in the PFC and hippocampus, D₁ receptor expression decreased in the NAc, and DAT levels decreased in the caudate putamen (CPu), and elevated in the VTA. These results suggest that early treatment with various APDs can cause different long-term alterations in the adult brain, across both treatment groups and genders. Full article

(This article belongs to the Special Issue Antipsychotics)

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16 pages, 374 KiB

Open AccessReview

Neuroprotective Strategies during Cardiac Surgery with Cardiopulmonary Bypass

by Aida Salameh^1,*, Stefan Dhein², Ingo Dähnert¹ and Norbert Klein³

¹ Clinic for Paediatric Cardiology Heart Centre, University of Leipzig, 04289 Leipzig, Germany

² Rudolf-Boehm-Institute for Pharmacology and Toxicology, University of Leipzig, 04107 Leipzig, Germany

³ Department of Cardiology, Angiology and Internal Intensive Care Medicine, St. Georg Hospital, Academic Medical Centre, University of Leipzig, 04129 Leipzig, Germany

Int. J. Mol. Sci. 2016, 17(11), 1945; https://doi.org/10.3390/ijms17111945 - 21 Nov 2016

Cited by 48 | Viewed by 12224

Abstract

Aortocoronary bypass or valve surgery usually require cardiac arrest using cardioplegic solutions. Although, in principle, in a number of cases beating heart surgery (so-called off-pump technique) is possible, aortic or valve surgery or correction of congenital heart diseases mostly require cardiopulmonary arrest. During [...] Read more.

Aortocoronary bypass or valve surgery usually require cardiac arrest using cardioplegic solutions. Although, in principle, in a number of cases beating heart surgery (so-called off-pump technique) is possible, aortic or valve surgery or correction of congenital heart diseases mostly require cardiopulmonary arrest. During this condition, the heart-lung machine also named cardiopulmonary bypass (CPB) has to take over the circulation. It is noteworthy that the invention of a machine bypassing the heart and lungs enabled complex cardiac operations, but possible negative effects of the CPB on other organs, especially the brain, cannot be neglected. Thus, neuroprotection during CPB is still a matter of great interest. In this review, we will describe the impact of CPB on the brain and focus on pharmacological and non-pharmacological strategies to protect the brain. Full article

(This article belongs to the Special Issue Neuroprotective Strategies 2016)

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18 pages, 3095 KiB

Open AccessReview

Prediction of Protein–Protein Interactions by Evidence Combining Methods

by Ji-Wei Chang^1,2, Yan-Qing Zhou^1,2, Muhammad Tahir Ul Qamar^1,2

, Ling-Ling Chen^1,2 and Yu-Duan Ding^1,2,*

¹ National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China

² College of Informatics, Huazhong Agricultural University, Wuhan 430070, China

Int. J. Mol. Sci. 2016, 17(11), 1946; https://doi.org/10.3390/ijms17111946 - 22 Nov 2016

Cited by 28 | Viewed by 14381

Abstract

Most cellular functions involve proteins’ features based on their physical interactions with other partner proteins. Sketching a map of protein–protein interactions (PPIs) is therefore an important inception step towards understanding the basics of cell functions. Several experimental techniques operating in vivo or in [...] Read more.

Most cellular functions involve proteins’ features based on their physical interactions with other partner proteins. Sketching a map of protein–protein interactions (PPIs) is therefore an important inception step towards understanding the basics of cell functions. Several experimental techniques operating in vivo or in vitro have made significant contributions to screening a large number of protein interaction partners, especially high-throughput experimental methods. However, computational approaches for PPI predication supported by rapid accumulation of data generated from experimental techniques, 3D structure definitions, and genome sequencing have boosted the map sketching of PPIs. In this review, we shed light on in silico PPI prediction methods that integrate evidence from multiple sources, including evolutionary relationship, function annotation, sequence/structure features, network topology and text mining. These methods are developed for integration of multi-dimensional evidence, for designing the strategies to predict novel interactions, and for making the results consistent with the increase of prediction coverage and accuracy. Full article

(This article belongs to the Special Issue Proteins and Protein-Ligand Interactions)

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16 pages, 1379 KiB

Open AccessArticle

Effects of Luteolin and Quercetin in Combination with Some Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus

by Muhammad Usman Amin^1,7, Muhammad Khurram^2,*, Taj Ali Khan¹, Hani S. Faidah³, Zia Ullah Shah⁴, Shafiq Ur Rahman², Abdul Haseeb⁵

, Muhammad Ilyas⁴, Naseem Ullah⁶, Sahibzada Muhammad Umar Khayam⁶ and Marcello Iriti^8,*

¹ Department of Microbiology, Kohat University of Science and Technology, Kohat 26000, Pakistan

² Department of Pharmacy, Shaheed Benazir Bhutto University, Sheringal 18000, Pakistan

³ Department of Microbiology, Faculty of Medicine, Umm Al Qura University, Makkah 21955, Saudi Arabia

⁴ Department of Microbiology & Biotechnology, Sarhad University of Science and Information Technology, Peshawar 25000, Pakistan

⁵ Department of Clinical Pharmacy, College of Pharmacy, Umm Al Qura University, Makkah 21955, Saudi Arabia

⁶ Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar 25000, Pakistan

⁷ Department of Pharmacology, Khyber Medical University Institute of Medical Sciences, Kohat 26000, Pakistan

⁸ Department of Agricultural and Environmental Sciences, Milan State University, 20133 Milan, Italy

Int. J. Mol. Sci. 2016, 17(11), 1947; https://doi.org/10.3390/ijms17111947 - 22 Nov 2016

Cited by 69 | Viewed by 10748

Abstract

The present study was designed to evaluate the effects of flavonoids luteolin (L) and quercetin + luteolin (Q + L) in combination with commonly used antibacterial agents against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates and S. aureus (ATCC 43300). Minimum inhibitory concentrations (MICs) [...] Read more.

The present study was designed to evaluate the effects of flavonoids luteolin (L) and quercetin + luteolin (Q + L) in combination with commonly used antibacterial agents against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates and S. aureus (ATCC 43300). Minimum inhibitory concentrations (MICs) of L and Q + L, as well as the MICs of flavonoids in combination with antibiotics were determined and results showed an increased activity of flavonoids with antibiotics. The synergistic, additive, or antagonistic relationships between flavonoids (L and Q + L) and antibiotics were also evaluated, and additive and synergistic effects were observed for some antibiotic + flavonoid combinations. In addition, some combinations were also found to damage the bacterial cytoplasmic membrane, as assessed through potassium leakage assay. The effects of flavonoids and flavonoids + antibiotics on mecA gene mutations were also tested, and no functional variation was detected in the coding region. Full article

(This article belongs to the Special Issue Bioactive Phytochemicals and Functional Food Ingredients in Fruits and Vegetables 2016)

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12 pages, 2951 KiB

Open AccessArticle

Electric Signals Regulate the Directional Migration of Oligodendrocyte Progenitor Cells (OPCs) via β1 Integrin

by Bangfu Zhu^1,2, Matthew Nicholls¹, Yu Gu¹, Gaofeng Zhang¹, Chao Zhao³, Robin J. M. Franklin³ and Bing Song^1,4,*

¹ Cardiff Institute of Tissue Engineering and Repair, School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff CF14 4XY, UK

² School of Biochemistry, University of Bristol, Bristol BS8 1TD, UK

³ Wellcome Trust—Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1TA, UK

⁴ Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang 110001, China

Int. J. Mol. Sci. 2016, 17(11), 1948; https://doi.org/10.3390/ijms17111948 - 22 Nov 2016

Cited by 21 | Viewed by 5969

Abstract

The guided migration of neural cells is essential for repair in the central nervous system (CNS). Oligodendrocyte progenitor cells (OPCs) will normally migrate towards an injury site to re-sheath demyelinated axons; however the mechanisms underlying this process are not well understood. Endogenous electric [...] Read more.

The guided migration of neural cells is essential for repair in the central nervous system (CNS). Oligodendrocyte progenitor cells (OPCs) will normally migrate towards an injury site to re-sheath demyelinated axons; however the mechanisms underlying this process are not well understood. Endogenous electric fields (EFs) are known to influence cell migration in vivo, and have been utilised in this study to direct the migration of OPCs isolated from neonatal Sprague-Dawley rats. The OPCs were exposed to physiological levels of electrical stimulation, and displayed a marked electrotactic response that was dependent on β1 integrin, one of the key subunits of integrin receptors. We also observed that F-actin, an important component of the cytoskeleton, was re-distributed towards the leading edge of the migrating cells, and that this asymmetric rearrangement was associated with β1 integrin function. Full article

(This article belongs to the Special Issue Pluripotent Stem Cell Technology for Disease Modeling, Drug Discovery and Regenerative Medicine)

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14 pages, 3108 KiB

Open AccessReview

Structure-Functional Basis of Ion Transport in Sodium–Calcium Exchanger (NCX) Proteins

by Moshe Giladi^1,2,*

, Reut Shor¹, Michal Lisnyansky¹ and Daniel Khananshvili^1,*

¹ Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 699780, Israel

² Tel-Aviv Sourasky Medical Center, Tel-Aviv 6423906, Israel

Int. J. Mol. Sci. 2016, 17(11), 1949; https://doi.org/10.3390/ijms17111949 - 22 Nov 2016

Cited by 41 | Viewed by 9170

Abstract

The membrane-bound sodium–calcium exchanger (NCX) proteins shape Ca²⁺ homeostasis in many cell types, thus participating in a wide range of physiological and pathological processes. Determination of the crystal structure of an archaeal NCX (NCX_Mj) paved the way for a thorough and systematic [...] Read more.

The membrane-bound sodium–calcium exchanger (NCX) proteins shape Ca²⁺ homeostasis in many cell types, thus participating in a wide range of physiological and pathological processes. Determination of the crystal structure of an archaeal NCX (NCX_Mj) paved the way for a thorough and systematic investigation of ion transport mechanisms in NCX proteins. Here, we review the data gathered from the X-ray crystallography, molecular dynamics simulations, hydrogen–deuterium exchange mass-spectrometry (HDX-MS), and ion-flux analyses of mutants. Strikingly, the apo NCX_Mj protein exhibits characteristic patterns in the local backbone dynamics at particular helix segments, thereby possessing characteristic HDX profiles, suggesting structure-dynamic preorganization (geometric arrangements of catalytic residues before the transition state) of conserved α₁ and α₂ repeats at ion-coordinating residues involved in transport activities. Moreover, dynamic preorganization of local structural entities in the apo protein predefines the status of ion-occlusion and transition states, even though Na⁺ or Ca²⁺ binding modifies the preceding backbone dynamics nearby functionally important residues. Future challenges include resolving the structural-dynamic determinants governing the ion selectivity, functional asymmetry and ion-induced alternating access. Taking into account the structural similarities of NCX_Mj with the other proteins belonging to the Ca²⁺/cation exchanger superfamily, the recent findings can significantly improve our understanding of ion transport mechanisms in NCX and similar proteins. Full article

(This article belongs to the Special Issue Metalloproteins 2017)

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13 pages, 7527 KiB

Open AccessArticle

Regulation of Intrinsic and Extrinsic Apoptotic Pathways in Osteosarcoma Cells Following Oleandrin Treatment

by Yunlong Ma^1,†, Bin Zhu^2,†, Lei Yong^1,†, Chunyu Song³, Xiao Liu¹, Huilei Yu¹, Peng Wang¹, Zhongjun Liu¹ and Xiaoguang Liu^1,*

¹ Department of Orthopedics, Peking University Third Hospital, No. 49, North Garden Street, Haidian District, Beijing 100191, China

² The Center for Pain Medicine, Peking University Third Hospital, No. 49, North Garden Street, Haidian District, Beijing 100191, China

³ Department of Anesthesiology, Peking University Third Hospital, No. 49, North Garden Street, Haidian District, Beijing 100191, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1950; https://doi.org/10.3390/ijms17111950 - 23 Nov 2016

Cited by 34 | Viewed by 8210

Abstract

Our previous study has reported the anti-tumor effect of oleandrin on osteosarcoma (OS) cells. In the current study, we mainly explored its potential regulation on intrinsic and extrinsic apoptotic pathway in OS cells. Cells apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential [...] Read more.

Our previous study has reported the anti-tumor effect of oleandrin on osteosarcoma (OS) cells. In the current study, we mainly explored its potential regulation on intrinsic and extrinsic apoptotic pathway in OS cells. Cells apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were detected using fluorescence staining and flow cytometry. Caspase-3 activity was detected using a commercial kit. The levels of cytoplasmic cytochrome c, mitochondrial cytochrome c, bcl-2, bax, caspase-9, Fas, FasL, caspase-8 and caspase-3 were detected by Western blotting. z-VAD-fmk was applied to block both intrinsic and extrinsic apoptosis pathways, and cells apoptosis was also tested. Furthermore, we used z-LEHD-fmk and Fas blocking antibody to inhibit intrinsic and extrinsic pathways, separately, and the selectivity of oleandrin on these pathways was explored. Results showed that oleandrin induced the apoptosis of OS cells, which was accompanied by an increase in ROS and a decrease in MMP. Furthermore, cytochrome c level was reduced in mitochondria but elevated in the cytoplasm. Caspase-3 activity was enhanced by oleandrin in a concentration- and time-dependent manner. Oleandrin also down-regulated the expression of bcl-2, but up-regulated bax, caspase-9, Fas, FasL, caspase-8 and caspase-3. In addition, the suppression of both apoptotic pathways by z-VAD-fmk greatly reverted the oleandrin-induced apoptosis. Moreover, the suppression of one pathway by a corresponding inhibitor did not affect the regulation of oleandrin on another pathway. Taken together, we concluded that oleandrin induced apoptosis of OS cells via activating both intrinsic and extrinsic apoptotic pathways. Full article

(This article belongs to the Section Bioactives and Nutraceuticals)

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14 pages, 7202 KiB

Open AccessCommunication

Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment

by Yali Liu¹

, Suying Han², Xiangming Ding³, Xinmin Li^1,3, Lifeng Zhang¹, Wanfeng Li¹

, Haiyan Xu¹, Zhexin Li¹ and Liwang Qi^1,*

¹ State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

² Key Laboratory of Research Institute of Forest Ecology and Protection, Chinese Academy of Forestry, Beijing 100091, China

³ Department of Pathology and Laboratory Medicine, University of California at Los Angeles, 1000 Veteran Ave., Los Angeles, CA 90095, USA

Int. J. Mol. Sci. 2016, 17(11), 1951; https://doi.org/10.3390/ijms17111951 - 22 Nov 2016

Cited by 16 | Viewed by 6066

Abstract

Hydrogen is a therapeutic antioxidant that has been used extensively in clinical trials. It also acts as a bioactive molecule that can alleviate abiotic stress in plants. However, the biological effects of hydrogen in somatic embryos and the underlying molecular basis remain largely [...] Read more.

Hydrogen is a therapeutic antioxidant that has been used extensively in clinical trials. It also acts as a bioactive molecule that can alleviate abiotic stress in plants. However, the biological effects of hydrogen in somatic embryos and the underlying molecular basis remain largely unknown. In this study, the morphological and physiological influence of exogenous H₂ treatment during somatic embryogenesis was characterized in Larix leptolepis Gordon. The results showed that exposure to hydrogen increased the proportions of active pro-embryogenic cells and normal somatic embryos. We sequenced mRNA and microRNA (miRNA) libraries to identify global transcriptome changes at different time points during H₂ treatment of larch pro-embryogenic masses (PEMs). A total of 45,393 mRNAs and 315 miRNAs were obtained. Among them, 4253 genes and 96 miRNAs were differentially expressed in the hydrogen-treated libraries compared with the control. Further, a large number of the differentially expressed mRNAs and miRNAs were related to reactive oxygen species (ROS) homeostasis and cell cycle regulation. We also identified 4399 potential target genes for 285 of the miRNAs. The differential expression data and the mRNA-miRNA interaction network described here provide new insights into the molecular mechanisms that determine the performance of PEMs exposed to H₂ during somatic embryogenesis. Full article

(This article belongs to the Section Molecular Plant Sciences)

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15 pages, 1774 KiB

Open AccessReview

Calcium Dyshomeostasis in Tubular Aggregate Myopathy

by Jong-Mok Lee^1,2

and Satoru Noguchi^1,2,*

¹ Department of Genome Medicine Development, Medical Genome Center, National Center of Neurology and Neuropsychiatry, Kodaira, Tokyo 187-8551, Japan

² Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Neuropsychiatry, Kodaira, Tokyo 187-8502, Japan

Int. J. Mol. Sci. 2016, 17(11), 1952; https://doi.org/10.3390/ijms17111952 - 22 Nov 2016

Cited by 14 | Viewed by 8941

Abstract

Calcium is a crucial mediator of cell signaling in skeletal muscles for basic cellular functions and specific functions, including contraction, fiber-type differentiation and energy production. The sarcoplasmic reticulum (SR) is an organelle that provides a large supply of intracellular Ca²⁺ in myofibers. [...] Read more.

Calcium is a crucial mediator of cell signaling in skeletal muscles for basic cellular functions and specific functions, including contraction, fiber-type differentiation and energy production. The sarcoplasmic reticulum (SR) is an organelle that provides a large supply of intracellular Ca²⁺ in myofibers. Upon excitation, it releases Ca²⁺ into the cytosol, inducing contraction of myofibrils. During relaxation, it takes up cytosolic Ca²⁺ to terminate the contraction. During exercise, Ca²⁺ is cycled between the cytosol and the SR through a system by which the Ca²⁺ pool in the SR is restored by uptake of extracellular Ca²⁺ via a specific channel on the plasma membrane. This channel is called the store-operated Ca²⁺ channel or the Ca²⁺ release-activated Ca²⁺ channel. It is activated by depletion of the Ca²⁺ store in the SR by coordination of two main molecules: stromal interaction molecule 1 (STIM1) and calcium release-activated calcium channel protein 1 (ORAI1). Recently, myopathies with a dominant mutation in these genes have been reported and the pathogenic mechanism of such diseases have been proposed. This review overviews the calcium signaling in skeletal muscles and role of store-operated Ca²⁺ entry in calcium homeostasis. Finally, we discuss the phenotypes and the pathomechanism of myopathies caused by mutations in the STIM1 and ORAI1 genes. Full article

(This article belongs to the Special Issue Calcium Regulation and Sensing)

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20 pages, 1663 KiB

Open AccessReview

d-Lysergic Acid Diethylamide (LSD) as a Model of Psychosis: Mechanism of Action and Pharmacology

by Danilo De Gregorio¹, Stefano Comai², Luca Posa¹

and Gabriella Gobbi^1,*

¹ Neurobiological Psychiatry Unit, McGill University, Montreal, QC H3A 1A1, Canada

² Division of Neuroscience, San Raffaele Scientific Institute and Vita-Salute University, 20132 Milan, Italy

Int. J. Mol. Sci. 2016, 17(11), 1953; https://doi.org/10.3390/ijms17111953 - 23 Nov 2016

Cited by 102 | Viewed by 46479

Abstract

d-Lysergic Acid Diethylamide (LSD) is known for its hallucinogenic properties and psychotic-like symptoms, especially at high doses. It is indeed used as a pharmacological model of psychosis in preclinical research. The goal of this review was to understand the mechanism of action [...] Read more.

d-Lysergic Acid Diethylamide (LSD) is known for its hallucinogenic properties and psychotic-like symptoms, especially at high doses. It is indeed used as a pharmacological model of psychosis in preclinical research. The goal of this review was to understand the mechanism of action of psychotic-like effects of LSD. We searched Pubmed, Web of Science, Scopus, Google Scholar and articles’ reference lists for preclinical studies regarding the mechanism of action involved in the psychotic-like effects induced by LSD. LSD’s mechanism of action is pleiotropic, primarily mediated by the serotonergic system in the Dorsal Raphe, binding the 5-HT_2A receptor as a partial agonist and 5-HT_1A as an agonist. LSD also modulates the Ventral Tegmental Area, at higher doses, by stimulating dopamine D₂, Trace Amine Associate receptor 1 (TAAR₁) and 5-HT_2A. More studies clarifying the mechanism of action of the psychotic-like symptoms or psychosis induced by LSD in humans are needed. LSD’s effects are mediated by a pleiotropic mechanism involving serotonergic, dopaminergic, and glutamatergic neurotransmission. Thus, the LSD-induced psychosis is a useful model to test the therapeutic efficacy of potential novel antipsychotic drugs, particularly drugs with dual serotonergic and dopaminergic (DA) mechanism or acting on TAAR₁ receptors. Full article

(This article belongs to the Special Issue Antipsychotics)

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11 pages, 853 KiB

Open AccessArticle

Pulmonary Function and Incidence of Selected Respiratory Diseases Depending on the Exposure to Ambient PM₁₀

by Artur Badyda^1,5,*

, Anna Gayer¹, Piotr Oskar Czechowski², Grzegorz Majewski³

and Piotr Dąbrowiecki^4,5

¹ Faculty of Building Services, Hydro- and Environmental Engineering, Warsaw University of Technology, 20 Nowowiejska St., PL00-653 Warsaw, Poland

² Department of Information Systems, Gdynia Maritime University, 83 Morska St., PL81-225 Gdynia, Poland

³ Faculty of Civil and Environmental Engineering, Warsaw University of Life Sciences, 159 Nowoursynowska St., PL02-776 Warsaw, Poland

⁴ Military Institute of Medicine, Central Clinical Hospital of the Ministry of National Defense, 128 Szaserów St., PL04-141 Warsaw, Poland

⁵ Polish Federation of Asthma, Allergy and COPD Patients’ Associations, 23/102 Łabiszyńska St., PL03-204 Warsaw, Poland

Int. J. Mol. Sci. 2016, 17(11), 1954; https://doi.org/10.3390/ijms17111954 - 22 Nov 2016

Cited by 36 | Viewed by 5780

Abstract

It is essential in pulmonary disease research to take into account traffic-related air pollutant exposure among urban inhabitants. In our study, 4985 people were examined for spirometric parameters in the presented research which was conducted in the years 2008–2012. The research group was [...] Read more.

It is essential in pulmonary disease research to take into account traffic-related air pollutant exposure among urban inhabitants. In our study, 4985 people were examined for spirometric parameters in the presented research which was conducted in the years 2008–2012. The research group was divided into urban and rural residents. Traffic density, traffic structure and velocity, as well as concentrations of selected air pollutants (CO, NO₂ and PM₁₀) were measured at selected areas. Among people who live in the city, lower percentages of predicted values of spirometric parameters were noticed in comparison to residents of rural areas. Taking into account that the difference in the five-year mean concentration of PM₁₀ in the considered city and rural areas was over 17 μg/m³, each increase of PM₁₀ by 10 μg/m³ is associated with the decline in FEV₁ (forced expiratory volume during the first second of expiration) by 1.68%. These findings demonstrate that traffic-related air pollutants may have a significant influence on the decline of pulmonary function and the growing rate of respiratory diseases. Full article

(This article belongs to the Special Issue Molecular Research on Global Climate Change and Atmospheric Pollution)

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10 pages, 1876 KiB

Open AccessCommunication

Importance of Heat and Pressure for Solubilization of Recombinant Spider Silk Proteins in Aqueous Solution

by Justin A. Jones¹, Thomas I. Harris²

, Paula F. Oliveira¹, Brianne E. Bell¹, Abdulrahman Alhabib¹ and Randolph V. Lewis^1,*

¹ Department of Biology, Utah State University, Logan, UT 84341, USA

² Department of Biological Engineering, Utah State University, Logan, UT 84341, USA

Int. J. Mol. Sci. 2016, 17(11), 1955; https://doi.org/10.3390/ijms17111955 - 23 Nov 2016

Cited by 8 | Viewed by 6383

Abstract

The production of recombinant spider silk proteins continues to be a key area of interest for a number of research groups. Several key obstacles exist in their production as well as in their formulation into useable products. The original reported method to solubilize [...] Read more.

The production of recombinant spider silk proteins continues to be a key area of interest for a number of research groups. Several key obstacles exist in their production as well as in their formulation into useable products. The original reported method to solubilize recombinant spider silk proteins (rSSp) in an aqueous solution involved using microwaves to quickly generate heat and pressure inside of a sealed vial containing rSSp and water. Fibers produced from this system are remarkable in their mechanical ability and demonstrate the ability to be stretched and recover 100 times. The microwave method dissolves the rSSPs with dissolution time increasing with higher molecular weight constructs, increasing concentration of rSSPs, protein type, and salt concentration. It has proven successful in solvating a number of different rSSPs including native-like sequences (MaSp1, MaSp2, piriform, and aggregate) as well as chimeric sequences (FlAS) in varied concentrations that have been spun into fibers and formed into films, foams, sponges, gels, coatings, macro and micro spheres and adhesives. The system is effective but inherently unpredictable and difficult to control. Provided that the materials that can be generated from this method of dissolution are impressive, an alternative means of applying heat and pressure that is controllable and predictable has been developed. Results indicate that there are combinations of heat and pressure (135 °C and 140 psi) that result in maximal dissolution without degrading the recombinant MaSp2 protein tested, and that heat and pressure are the key elements to the method of dissolution. Full article

(This article belongs to the Special Issue Silk-Based Materials: From Production to Characterization)

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14 pages, 3397 KiB

Open AccessArticle

Microarray Expression Profiling of Long Non-Coding RNAs Involved in Nasopharyngeal Carcinoma Metastasis

by Xin Wen^†, Xinran Tang^†, Yingqin Li, Xianyue Ren, Qingmei He, Xiaojing Yang, Jian Zhang, Yaqin Wang, Jun Ma^* and Na Liu^*

¹ Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou 510060, China

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1956; https://doi.org/10.3390/ijms17111956 - 23 Nov 2016

Cited by 31 | Viewed by 6971

Abstract

Increasing evidence has demonstrated a significant role for long non-coding RNAs (lncRNAs) in tumorigenesis. However, their functions in nasopharyngeal carcinoma (NPC) metastasis remain largely unknown. In this study, a model comparing high and low metastatic NPC cell lines (5-8F vs. 6-10B and S18 [...] Read more.

Increasing evidence has demonstrated a significant role for long non-coding RNAs (lncRNAs) in tumorigenesis. However, their functions in nasopharyngeal carcinoma (NPC) metastasis remain largely unknown. In this study, a model comparing high and low metastatic NPC cell lines (5-8F vs. 6-10B and S18 vs. S26) was constructed to determine the expression profile of lncRNAs using the microarray analysis, and we found 167 lncRNAs and 209 mRNAs were differentially expressed. Bioinformatic analysis indicated that the dysregulated mRNAs participated in important biological regulatory functions in NPC. Validation of 26 significantly dysregulated lncRNAs by qRT-PCR showed the expression patterns of 22 lncRNAs were in accordance with the microarray data. Furthermore, the expression level of ENST00000470135, which was the most upregulated lncRNA in high metastatic cell lines, was significantly higher in NPC cell lines and tissues with lymph node metastasis (LNM) and knocking down ENST00000470135 suppressed the migration, invasion and proliferation of NPC cells in vitro. In conclusion, our study revealed expression patterns of lncRNAs in NPC metastasis. The dysregulated lncRNAs may act as novel biomarkers and therapeutic targets for NPC. Full article

(This article belongs to the Collection Regulation by Non-coding RNAs)

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22 pages, 8560 KiB

Open AccessArticle

Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering

by Chih-Hao Chen^1,2,†

, Chang-Yi Kuo^1,†, Yan-Jie Wang¹ and Jyh-Ping Chen^1,2,3,4,*

¹ Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan 33302, Taiwan

² Department of Plastic and Reconstructive Surgery and Craniofacial Research Center, Chang Gung Memorial Hospital, Kwei-San, Taoyuan 33305, Taiwan

³ Graduate Institute of Health Industry and Technology, Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Kwei-San, Taoyuan 33302, Taiwan

⁴ Department of Materials Engineering, Ming Chi University of Technology, Tai-Shan, New Taipei City 24301, Taiwan

^† These authors contribute equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1957; https://doi.org/10.3390/ijms17111957 - 23 Nov 2016

Cited by 56 | Viewed by 8191

Abstract

Glucosamine (GlcN) fulfills many of the requirements as an ideal component in scaffolds used in cartilage tissue engineering. The incorporation of GlcN in a gelatin/hyaluronic acid (GH) cryogel scaffold could provide biological cues in maintaining the phenotype of chondrocytes. Nonetheless, substituting gelatin with [...] Read more.

Glucosamine (GlcN) fulfills many of the requirements as an ideal component in scaffolds used in cartilage tissue engineering. The incorporation of GlcN in a gelatin/hyaluronic acid (GH) cryogel scaffold could provide biological cues in maintaining the phenotype of chondrocytes. Nonetheless, substituting gelatin with GlcN may also decrease the crosslinking density and modulate the mechanical properties of the cryogel scaffold, which may be beneficial as physical cues for chondrocytes in the scaffold. Thus, we prepared cryogel scaffolds containing 9% GlcN (GH-GlcN9) and 16% GlcN (GH-GlcN16) by carbodiimide-mediated crosslinking reactions at −16 °C. The crosslinking density and the mechanical properties of the cryogel matrix could be tuned by adjusting the content of GlcN used during cryogel preparation. In general, incorporation of GlcN did not influence scaffold pore size and ultimate compressive strain but increased porosity. The GH-GlcN16 cryogel showed the highest swelling ratio and degradation rate in hyaluronidase and collagenase solutions. On the contrary, the Young’s modulus, storage modulus, ultimate compressive stress, energy dissipation level, and rate of stress relaxation decreased by increasing the GlcN content in the cryogel. The release of GlcN from the scaffolds in the culture medium of chondrocytes could be sustained for 21 days for GH-GlcN16 in contrast to only 7 days for GH-GlcN9. In vitro cell culture experiments using rabbit articular chondrocytes revealed that GlcN incorporation affected cell proliferation, morphology, and maintenance of chondrogenic phenotype. Overall, GH-GlcN16 showed the best performance in maintaining chondrogenic phenotype with reduced cell proliferation rate but enhanced glycosaminoglycans (GAGs) and type II collagen (COL II) secretion. Quantitative real-time polymerase chain reaction also showed time-dependent up-regulation of cartilage-specific marker genes (COL II, aggrecan and Sox9) for GH-GlcN16. Implantation of chondrocytes/GH-GlcN16 constructs into full-thickness articular cartilage defects of rabbits could regenerate neocartilage with positive staining for GAGs and COL II. The GH-GlcN16 cryogel will be suitable as a scaffold for the treatment of articular cartilage defects. Full article

(This article belongs to the Section Materials Science)

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28 pages, 6646 KiB

Open AccessReview

Pro-Tumoral Inflammatory Myeloid Cells as Emerging Therapeutic Targets

by Gabor J. Szebeni^1,2,*

, Csaba Vizler³, Lajos I. Nagy¹, Klara Kitajka⁴ and Laszlo G. Puskas^1,4

¹ Avidin Ltd., Also kikoto sor 11/D., H-6726 Szeged, Hungary

² Synaptogenex Ltd., Őzsuta utca 20995/1, H-1037 Budapest, Hungary

³ Department of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Temesvari krt. 62., H-6726 Szeged, Hungary

⁴ Department of Genetics, Biological Research Center, Hungarian Academy of Sciences, Temesvari krt. 62., H-6726 Szeged, Hungary

Int. J. Mol. Sci. 2016, 17(11), 1958; https://doi.org/10.3390/ijms17111958 - 23 Nov 2016

Cited by 45 | Viewed by 8570

Abstract

Since the observation of Virchow, it has long been known that the tumor microenvironment constitutes the soil for the infiltration of inflammatory cells and for the release of inflammatory mediators. Under certain circumstances, inflammation remains unresolved and promotes cancer development. Here, we review [...] Read more.

Since the observation of Virchow, it has long been known that the tumor microenvironment constitutes the soil for the infiltration of inflammatory cells and for the release of inflammatory mediators. Under certain circumstances, inflammation remains unresolved and promotes cancer development. Here, we review some of these indisputable experimental and clinical evidences of cancer related smouldering inflammation. The most common myeloid infiltrate in solid tumors is composed of myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs). These cells promote tumor growth by several mechanisms, including their inherent immunosuppressive activity, promotion of neoangiogenesis, mediation of epithelial-mesenchymal transition and alteration of cellular metabolism. The pro-tumoral functions of TAMs and MDSCs are further enhanced by their cross-talk offering a myriad of potential anti-cancer therapeutic targets. We highlight these main pro-tumoral mechanisms of myeloid cells and give a general overview of their phenotypical and functional diversity, offering examples of possible therapeutic targets. Pharmacological targeting of inflammatory cells and molecular mediators may result in therapies improving patient condition and prognosis. Here, we review experimental and clinical findings on cancer-related inflammation with a major focus on creating an inventory of current small molecule-based therapeutic interventions targeting cancer-related inflammatory cells: TAMs and MDSCs. Full article

(This article belongs to the Special Issue Inflammation and Cancer)

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12 pages, 3758 KiB

Open AccessArticle

Intracranial Thrombus Morphology and Composition Undergoes Time-Dependent Changes in Acute Ischemic Stroke: A CT Densitometry Study

by Slaven Pikija¹, Jozef Magdic², Vladimir Trkulja³, Peter Unterkreuter⁴, Johannes Sebastian Mutzenbach¹, Helmut F. Novak¹, Friedrich Weymayr⁵, Larissa Hauer^6,† and Johann Sellner^1,7,8,*,†

¹ Department of Neurology, Christian Doppler Medical Center, Paracelsus Medical University, 5020 Salzburg, Austria

² Department of Neurology, Univerzitetni Klinični Center, 2000 Maribor, Slovenia

³ Department for Pharmacology, School of Medicine, University of Zagreb, 10000 Zagreb, Croatia

⁴ Department of Neurology, Bezirkskrankenhaus Lienz, 9900 Lienz, Austria

⁵ Division of Neuroradiology, Christian Doppler Medical Center, Paracelsus Medical University, 5020 Salzburg, Austria

⁶ Department of Psychiatry, Christian Doppler Medical Center, Paracelsus Medical University, 5020 Salzburg, Austria

⁷ Department of Neurology, Klinikum rechts der Isar, Technische Universität, 81675 München, Germany

⁸ Institute of Linguistics, University of Salzburg, 5020 Salzburg, Austria

^† These authors contributed equally to this work.

Int. J. Mol. Sci. 2016, 17(11), 1959; https://doi.org/10.3390/ijms17111959 - 23 Nov 2016

Cited by 27 | Viewed by 5707

Abstract

The aim of our study was to assess whether cerebral artery clots undergo time-dependent morphological and compositional changes in acute ischemic stroke. We performed a retrospective chart review of patients admitted within 5 h from symptom onset to three European stroke centers and [...] Read more.

The aim of our study was to assess whether cerebral artery clots undergo time-dependent morphological and compositional changes in acute ischemic stroke. We performed a retrospective chart review of patients admitted within 5 h from symptom onset to three European stroke centers and evaluated non-contrast-enhanced CT (NECT) for hyperdense artery signs (HAS) in 2565 scans. The occlusion site, density of HAS expressed in Hounsfield units (HU), area of HAS, and relative density (rHU) (HU clot/HU non-affected artery) were studied and related to time from symptom onset, clinical severity, stroke etiology, and laboratory parameters. A HAS was present in the middle cerebral artery (MCA) in 185 (7.2%) and further explored. The mean time from symptom onset to CT was 100 min (range 17–300). We found a time-dependent loss of density in the occluded M1 segment within the first 5 h (N = 118, 95% CI [−15, −2], p = 0.01). Further, the thrombus area in the M2 segment decreased with time (cubic trend N = 67, 95% CI [−63, −8], p = 0.02). Overall, and especially in the M2 segment, a lower clot area was associated with higher fibrinogen (−21.7%, 95% CI [−34.8, −5.8], p = 0.009). In conclusion, our results disclosed time-dependent changes of intracranial thrombi with regard to occlusion site, density and area. Full article

(This article belongs to the Special Issue Atherosclerosis and Vascular Imaging 2016)

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20 pages, 796 KiB

Open AccessArticle

Fatty Acid and Phenolic Compound Concentrations in Eight Different Monovarietal Virgin Olive Oils from Extremadura and the Relationship with Oxidative Stability

by Alfonso Montaño¹, Marcos Hernández², Inmaculada Garrido³, José Luís Llerena^1,3 and Francisco Espinosa^3,*

¹ Agri-Food Technological Center of Extremadura-CTAEX, 06195 Badajoz, Spain

² Aula Dei Scientific Technological Park Foundation, Av. Montañana 930, 50059 Zaragoza, Spain

³ Department of Plant Biology, Ecology and Earth Sciences, University of Extremadura, 06006 Badajoz, Spain

Int. J. Mol. Sci. 2016, 17(11), 1960; https://doi.org/10.3390/ijms17111960 - 23 Nov 2016

Cited by 52 | Viewed by 6947

Abstract

Olive oils have been shown to be more resistant to oxidation than other vegetable fats, mainly due to their fatty acid (FA) profile which is rich in oleic acid and to their high content of antioxidants, principally phenols and tocopherols. This has situated [...] Read more.

Olive oils have been shown to be more resistant to oxidation than other vegetable fats, mainly due to their fatty acid (FA) profile which is rich in oleic acid and to their high content of antioxidants, principally phenols and tocopherols. This has situated virgin olive oils (VOOs) among the fats of high nutritional quality. However, it is important to stress that the oil’s commercial category (olive oil, virgin olive oil, extra-virgin olive oil), the variety of the source plant, and the extraction-conservation systems all decisively influence the concentration of these antioxidants and the oil’s shelf-life. The present work studied the fatty acid (FA) and phenolic composition and the oxidative stability (OS) of eight olive varieties grown in Extremadura (Arbequina, Cornicabra, Manzanilla Cacereña, Manzanilla de Sevilla, Morisca, Pico Limón, Picual, and Verdial de Badajoz), with the olives being harvested at different locations and dates. The Cornicabra, Picual, and Manzanilla Cacereña VOOs were found to have high oleic acid contents (>77.0%), while the VOOs of Morisca and Verdial de Badajoz had high linoleic acid contents (>14.5%). Regarding the phenol content, high values were found in the Cornicabra (633 mg·kg⁻¹) and Morisca (550 mg·kg⁻¹) VOOs, and low values in Arbequina (200 mg·kg⁻¹). The OS was found to depend upon both the variety and the date of harvesting. It was higher in the Cornicabra and Picual oils (>55 h), and lower in those of Verdial de Badajoz (26.3 h), Arbequina (29.8 h), and Morisca (31.5 h). In relating phenols and FAs with the OS, it was observed that, while the latter, particularly the linoleic content (R = −0.710, p < 0.001, n = 135), constitute the most influential factors, the phenolic compounds, especially o-diphenols, are equally influential when the oils’ linoleic content is ≥12.5% (R = 0.674, p < 0.001, n = 47). The results show that VOOs’ resistance to oxidation depends not only on the FA or phenolic profile, but also on the interaction of these compounds within the same matrix. Full article

(This article belongs to the Special Issue Bioactive Phytochemicals and Functional Food Ingredients in Fruits and Vegetables 2016)

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17 pages, 6367 KiB

Open AccessArticle

HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms

by Salunya Tancharoen¹, Satoshi Gando², Shrestha Binita³, Tomoka Nagasato³, Kiyoshi Kikuchi^1,4, Yuko Nawa⁵, Pornpen Dararat¹, Mika Yamamoto³, Somphong Narkpinit⁶ and Ikuro Maruyama^3,*

¹ Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand

² Department of Emergency and Critical Care, Hokkaido University, Kita-ku, Sapporo 0608648, Japan

³ Department of Systems Biology in Thromboregulation, Kagoshima University Graduate School of Medical and Dental Science, Kagoshima 8908544, Japan

⁴ Division of Brain Science, Department of Physiology, Kurume University School of Medicine, Asahi-machi, Kurume 8300011, Japan

⁵ Department of Anesthesiology and Intensive Care, Hokkaido Medical for Child Health and Rehabilitation, Sapporo 0060041, Japan

⁶ Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

Int. J. Mol. Sci. 2016, 17(11), 1961; https://doi.org/10.3390/ijms17111961 - 23 Nov 2016

Cited by 26 | Viewed by 7850

Abstract

High mobility group box 1 (HMGB1) is tightly connected to the process of tissue organization upon tissue injury. Here we show that HMGB1 controls epithelium and connective tissue regeneration both in vivo and in vitro during palatal wound healing. Heterozygous HMGB1 (Hmgb1 [...] Read more.

High mobility group box 1 (HMGB1) is tightly connected to the process of tissue organization upon tissue injury. Here we show that HMGB1 controls epithelium and connective tissue regeneration both in vivo and in vitro during palatal wound healing. Heterozygous HMGB1 (Hmgb1^+/−) mice and Wild-type (WT) mice were subjected to palatal injury. Maxillary tissues were stained with Mallory Azan or immunostained with anti-HMGB1, anti-proliferating cell nuclear antigen (PCNA), anti-nuclear factor-κB (NF-κB) p50 and anti-vascular endothelial growth factor (VEGF) antibodies. Palatal gingival explants were cultured with recombinant HMGB1 (rHMGB1) co-treated with siRNA targeting receptor for advanced glycation end products (RAGEs) for cell migration and PCNA expression analysis. Measurement of the wound area showed differences between Hmgb1^+/− and WT mice on Day 3 after wounding. Mallory Azan staining showed densely packed of collagen fibers in WT mice, whereas in Hmgb1^+/− mice weave-like pattern of low density collagen bundles were present. At three and seven days post-surgery, PCNA, NF-κB p50 and VEGF positive keratinocytes of WT mice were greater than that of Hmgb1^+/− mice. Knockdown of RAGE prevents the effect of rHMGB1-induced cell migration and PCNA expression in gingival cell cultures. The data suggest that HMGB1/RAGE axis has crucial roles in palatal wound healing. Full article

(This article belongs to the Special Issue Wound Repair and Regeneration)

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