SEPT12–NDC1 Complexes Are Required for Mammalian Spermiogenesis
Abstract
:1. Introduction
1.1. Male Infertility
1.2. SEPTINs (SEPTs) and Spermatogenesis
1.3. Nuclear Pore Complexes and NDC1
2. Results
2.1. NDC1 as a SEPT12 Interactor
2.2. Dynamic Expression of NDC1 during Murine Spermiogenesis
2.3. Effect of NDC1 on SEPT12 Localization and Filamental Structure in a Cell Model
2.4. Effect of Mutated SEPT12 on NDC1 Localization In Vivo
3. Discussion
3.1. SEPT12 Interaction with Nuclear and Nuclear-Related Proteins
3.2. Male Reproductive Roles of SEPT12 and NDC1
3.3. Effect of Mutated SEPT12 on Cellular Localization of NDC1
4. Materials and Methods
4.1. Immunofluorescence Assay
4.2. Cloning, Transfection, and Coimmunoprecipitation Assay
4.3. Separation of the Murine Testicular Germ Cell Populations and Sperm Preparation
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lai, T.-H.; Wu, Y.-Y.; Wang, Y.-Y.; Chen, M.-F.; Wang, P.; Chen, T.-M.; Wu, Y.-N.; Chiang, H.-S.; Kuo, P.-L.; Lin, Y.-H. SEPT12–NDC1 Complexes Are Required for Mammalian Spermiogenesis. Int. J. Mol. Sci. 2016, 17, 1911. https://doi.org/10.3390/ijms17111911
Lai T-H, Wu Y-Y, Wang Y-Y, Chen M-F, Wang P, Chen T-M, Wu Y-N, Chiang H-S, Kuo P-L, Lin Y-H. SEPT12–NDC1 Complexes Are Required for Mammalian Spermiogenesis. International Journal of Molecular Sciences. 2016; 17(11):1911. https://doi.org/10.3390/ijms17111911
Chicago/Turabian StyleLai, Tsung-Hsuan, Ying-Yu Wu, Ya-Yun Wang, Mei-Feng Chen, Pei Wang, Tsung-Ming Chen, Yi-No Wu, Han-Sun Chiang, Pao-Lin Kuo, and Ying-Hung Lin. 2016. "SEPT12–NDC1 Complexes Are Required for Mammalian Spermiogenesis" International Journal of Molecular Sciences 17, no. 11: 1911. https://doi.org/10.3390/ijms17111911