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Int. J. Mol. Sci. 2016, 17(11), 1799;

miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways

State Education Ministry’s Key Laboratory of Developmental Genes and Human Diseases, Southeast University, No. 2 Sipailou Road, Nanjing 210096, China
Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing 210009, China
Author to whom correspondence should be addressed.
Academic Editor: Andreas Keller
Received: 5 September 2016 / Revised: 18 October 2016 / Accepted: 21 October 2016 / Published: 27 October 2016
(This article belongs to the Special Issue microRNA Regulation 2017)
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MicroRNAs (miRNAs) are implicated in the regulation of tumor progression and stemness of cancer stem-like cells. Recently, miR-92a-3p was reported to be up-regulated in human glioma samples. Nevertheless, the precise role of miR-92a-3p in glioma cells and glioma stem-like cells (GSCs) has not been fully elucidated. It is necessary to clarify the function of miR-92a-3p in glioma and GSCs to develop novel therapeutic approaches for glioma patients. In the present study, we applied methyl-thiazolyl-tetrazolium (MTT) assay and Transwell assay to measure the proliferation rate and metastatic potential of glioma cells. Meanwhile, the self-renewal ability of GSCs was detected by tumor sphere formation assay. The results revealed that down-regulation of miR-92a-3p suppressed the glioma cell malignancy in vitro. Moreover, knockdown of miR-92a-3p led to a reduction of tumorgenesis in vivo. Interestingly, we also observed that up-regulation of miR-92a-3p could inhibit the stemness of GSCs. Subsequent mechanistic investigation indicated that cadherin 1 (CDH1)/β-catenin signaling and Notch-1/Akt signaling were the downstream pathways of miR-92a-3p in glioma cells and GSCs, respectively. These results suggest that miR-92a-3p plays different roles in glioma cells and GSCs through regulating different signaling pathways. View Full-Text
Keywords: glioma; glioma stem-like cells; miR-92a-3p; CDH1; Notch-1 glioma; glioma stem-like cells; miR-92a-3p; CDH1; Notch-1

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Song, H.; Zhang, Y.; Liu, N.; Zhao, S.; Kong, Y.; Yuan, L. miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways. Int. J. Mol. Sci. 2016, 17, 1799.

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