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Int. J. Mol. Sci. 2016, 17(11), 1873;

MicroRNAs 10a and 10b Regulate the Expression of Human Platelet Glycoprotein Ibα for Normal Megakaryopoiesis

School of Basic Medicine, Central South University, Changsha 410013, China
Cardiovascular Research Section, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
Author to whom correspondence should be addressed.
Academic Editor: Martin Pichler
Received: 17 August 2016 / Revised: 3 October 2016 / Accepted: 3 November 2016 / Published: 9 November 2016
(This article belongs to the Collection Regulation by Non-Coding RNAs)
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MicroRNAs are a class of small non-coding RNAs that bind to the three prime untranslated region (3′-UTR) of target mRNAs. They cause a cleavage or an inhibition of the translation of target mRNAs, thus regulating gene expression. Here, we employed three prediction tools to search for potential miRNA target sites in the 3′-UTR of the human platelet glycoprotein (GP) 1BA gene. A luciferase reporter assay shows that miR-10a and -10b sites are functional. When miR-10a or -10b mimics were transfected into the GP Ibβ/GP IX-expressing cells, along with a DNA construct harboring both the coding and 3′-UTR sequences of the human GP1BA gene, we found that they inhibit the transient expression of GP Ibα on the cell surface. When the miR-10a or -10b mimics were introduced into murine progenitor cells, upon megakaryocyte differentiation, we found that GP Ibα mRNA expression was markedly reduced, suggesting that a miRNA-induced mRNA degradation is at work. Thus, our study identifies GP Ibα as a novel target of miR-10a and -10b, suggesting that a drastic reduction in the levels of miR-10a and -10b in the late stage of megakaryopoiesis is required to allow the expression of human GP Ibα and the formation of the GP Ib-IX-V complex. View Full-Text
Keywords: microRNA; gene regulation; platelet glycoprotein Ibα microRNA; gene regulation; platelet glycoprotein Ibα

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Zhang, Z.; Ran, Y.; Shaw, T.S.; Peng, Y. MicroRNAs 10a and 10b Regulate the Expression of Human Platelet Glycoprotein Ibα for Normal Megakaryopoiesis. Int. J. Mol. Sci. 2016, 17, 1873.

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