Cell quantification is widely used both in basic and applied research. A typical example of its use is drug discovery research. Presently, plenty of methods for cell quantification are available. In this review, the basic techniques used for cell qua...
Polyacrylamide gel electrophoresis is widely used for studying proteins and protein-containing objects. However, it is employed most frequently as a qualitative method rather than a quantitative one. This paper shows the feasibility of routine digita...
Lipids are key molecules in various biological processes, thus their quantification is a crucial point in a lot of studies and should be taken into account in lipidomics development. This family is complex and presents a very large diversity of struc...
Credit scoring models are usually formulated by fitting the probability of loan default as a function of individual evaluation attributes. Typically, these attributes are measured using a Likert-type scale, but are treated as interval scale explanato...
The sensitivity of real-time PCR for hepatitis E virus (HEV) RNA quantification differs greatly among techniques. Standardized tools that measure the real quantity of virus are needed. We assessed the performance of a reverse transcription droplet di...
Methane emission estimates from offshore facilities are increasingly used for regulatory reporting and climate assessment, yet it remains unclear under what atmospheric conditions such estimates are physically meaningful. This study defines three nec...
Accurate and rapid quantification of (infectious) virus titers is of paramount importance in the manufacture of viral vectors and vaccines. Reliable quantification data allow efficient process development at a laboratory scale and thorough process mo...
Cisplatin, which kills cancer cells mainly through DNA crosslinking, has been widely used as a first-line chemotherapeutic agent although it also causes severe side effects. To improve anticancer outcomes, various types of cisplatin-based nanomedicin...
Tropomyosin is the major and predominant allergen among shellfish. This study developed an ultrasensitive immuno-PCR method for the quantification of crustacean tropomyosin in foods. The method couples sandwich ELISA with the real-time PCR (rtPCR) am...
The hydrolysis of raw food sources by commercially available food-grade enzymes releases thousands of peptides. The full characterization of bioactive hydrolysates requires robust methods to identify and quantify key peptides in these food sources. F...
Microplastics are an increasing concern due to their widespread occurrence in aquatic environments worldwide. The lack of a harmonised protocol for their reliable quantification remains a major challenge in current scientific efforts. This study pres...
SDN (Software-Defined Networking) renders network management more centralized and flexible. However, its control plane is vulnerable to attacks. By introducing dynamic heterogeneous redundancy, the amimic control plane is built to effectively solve t...
Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evap...
We identify a tonal contour in Turkish that expresses universal quantification. We show that the distribution of this contour is restricted to noun phrases modified by relative clauses and that it expresses universal quantification over situations ra...
Quantitative polymerase chain reaction (qPCR) is a widespread method to quantify RNA or DNA. The results are reported as cycle of quantification (Cq), scaled to absolute numbers of copies or relative to reference genes. The reported Cq values of the...
Deep learning is widely used in many real-life applications. Despite their remarkable performance accuracies, deep learning networks are often poorly calibrated, which could be harmful in risk-sensitive scenarios. Uncertainty quantification offers a...
Quantification of the actin cytoskeleton is of prime importance to unveil the cellular force sensing and transduction mechanism. Although fluorescence imaging provides a convenient tool for observing the morphology of the actin cytoskeleton, due to t...
Cyanogenic glycosides are naturally occurring compounds found in numerous plant species, which can release toxic hydrogen cyanide upon hydrolysis. The quantification of cyanogenic glycosides is essential for assessing their potential toxicity and hea...
To move towards a sustainable food system, we cannot continue to waste substantial amounts of the food produced. This is especially true for later stages in the food supply chain, where most sub-processes consume resources in vain when food is wasted...
The uncertainty quantification is an indispensable part for the validation of the nuclear safety best-estimate codes. However, the uncertainty quantification usually requires the combination of statistical analysis software and nuclear reactor profes...
Uncertainty quantification for complex deep learning models is increasingly important as these techniques see growing use in high-stakes, real-world settings. Currently, the quality of a model’s uncertainty is evaluated using point-prediction m...
Nitrogen reduction reaction (NRR) and nitrate reduction reaction (NO3−RR) provide a potential sustainable route by which to produce ammonia, a next-generation energy carrier. Many studies have been conducted over the years, mainly emphasizing m...
Sentiment analysis has been one of the most active research areas in the past decade due to its vast applications. Sentiment quantification, a new research problem in this field, extends sentiment analysis from individual documents to an aggregated c...
This paper provides a review of the present status of the topic dealt with. The contact and non-contact methods used for rock joint roughness measurement are summarized including their salient features, advantages, and disadvantages. A critical revie...
This paper reports on-line mixture quantification with FAIMS. Ternary gas mixtures composed of acetone, ethanol, and diethyl ether were used for quantification. We succeeded in an on-line quantification of ppm-level concentration and even sub-ppm-lev...
Chlorine is a widely used disinfectant and oxidant used for an array of municipal and industrial applications, including potable water, swimming pools, and cleaning of membranes. The most popular method to verify the concentration of free chlorine is...
The era of self-quantification in green consumption has dawned, encompassing everything from monitoring electricity usage to tracking carbon emissions. By leveraging technological tools to track self-related data pertaining to green behavioral activi...
Turboelectric systems can be considered complex systems that may comprise errors and uncertainty. Uncertainty quantification and error estimation processes can, therefore, be useful in achieving accurate system parameters. Uncertainty quantification...
Protein glycosylation governs key physiological and pathological processes in human cells. Aberrant glycosylation is thus closely associated with disease progression. Mass spectrometry (MS)-based glycoproteomics has emerged as an indispensable tool f...
Accurate DNA quantification is a highly important method within molecular biology. Methods widely used to quantify DNA are UV spectrometry and fluorometry. In this research, seven different DNA samples and one blank (MilliQ ultrapure water) were quan...
Ammonia (NH3), the most prevalent alkaline gas in the atmosphere, plays a significant role in PM2.5 formation, atmospheric chemistry, and new particle formation. This paper reviews quantification of [NH3] through measurements, satellite-remote-sensin...
Background: As essential biomolecules composed of proteins and carbohydrate moieties, glycoproteins play pivotal roles in numerous biological processes. The glycosylation level plays a crucial role in determining the functionality of glycoproteins. T...
Robust and selective quantification methods are required to better analyze feed supplementation effectiveness with specific amino acids. In this work, a reversed-phase high-performance liquid chromatography method with fluorescence detection is propo...
Since pre-industrial times, anthropogenic methane emissions have increased and are partly responsible for a changing global climate. Natural gas and oil extraction activities are one significant source of anthropogenic methane. While methods have bee...
The construction industry is known for poor performance, low productivity, high waste generation, and for lagging in the adoption of new technology. A high rate of material wastage in construction projects has economic and environmental implications...
Glutathione (GSH) is the most abundant low-molecular-weight biological thiol in vivo and has been linked to several diseases. The accurate quantification of GSH is therefore crucial for disease diagnosis and monitoring. In this study, we prepared sel...
The paper presents the novel method of damage identification and quantification in beams using the Wigner-Ville distribution (WVD). The presented non-parametric method is characterized by high sensitivity to a local stiffness decrease due to the pres...
Weather radar plays an important role in monitoring aerial animal migration, providing a stable data source for biological studies with large-scale coverage and consecutive-time samples. The accurate estimation of bird density from weather radar echo...
Due to the complexity and non-stationarity of the voice generation system, the nonlinearity of speech signals cannot be accurately quantified. Recently, the recurrence quantification analysis method has been used for voice disorder detection. In this...
Aortic stenosis (AS) is associated with the development of replacement myocardial fibrosis/scar. Given the dose-dependent relationship between scar and clinical outcomes after aortic valve replacement (AVR) surgery, scar quantity may serve as an impo...
Uncertainty quantification has proven to be an indispensable study for enhancing reliability and robustness of engineering systems in the early design phase. Single and multi-fidelity surrogate modelling methods have been used to replace the expensiv...
Given the widespread application of carbon fiber reinforced polymer (CFRP) structures in aerospace and automotive manufacturing, the critical role of structural health monitoring (SHM) is increasingly evident. Traditional damage quantification method...
Ultrasound-based strain imaging is available in several ultrasound (US) scanners. Strain ratio (SR) can be used to quantify the strain recorded simultaneously in two different user-selected areas, ideally exposed to the same amount of stress. The aim...
The credibility of Computational Fluid Dynamics (CFD) has been a topic of debate due to the significant uncertainties inherent in its modeling processes and numerical implementations. Uncertainty Quantification (UQ) offers a scientific framework for...
The mounting increase in the technological complexity of modern engineering systems requires compound uncertainty quantification, from a quantitative and qualitative perspective. This paper presents a Compound Uncertainty Quantification and Aggregati...
The numerical modeling of cardiac electrophysiology has reached a mature and advanced state that allows for quantitative modeling of many clinically relevant processes. As a result, complex computational tasks such as the creation of a variety of ele...
Hepatitis E virus (HEV) represents an emerging risk in industrialized countries where the consumption of contaminated food plays a pivotal role. Quantitative real-time RT-PCR (RT-qPCR) is one of the most suitable methods for the detection and quantif...
Traditional tone burst excitation cannot attain a high output resolution, due to the time duration. The received signal is much longer than that of excitation during the propagation, which can increase the difficulty of signal processing, and reduce...
High-throughput metabolomics can be used to optimize cell growth for enhanced production or for monitoring cell health in bioreactors. It has applications in cell and gene therapies, vaccines, biologics, and bioprocessing. NMR metabolomics is a metho...
The following study was conducted to generate a transgenic Sf9 cell line for rapid and easy virus quantification in the baculovirus expression system (BES). The hr3 (homologous region 3) and 39K and p10 promoters were used as the expression structure...
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