Biosensors

Rapid and reliable detection of biomarkers in complex fluids such as whole blood is essential for effective disease diagnosis and monitoring, particularly in point-of-care settings. Accordingly, this study developed a handheld lab-on-a-chip (LOC) platform that integrates on-chip plasma separation with label-free optical biosensing for real-time, dual-plex detection of biomarkers. The LOC platform includes a two-stage filtration unit that enables efficient separation of plasma from whole blood. This platform also includes a novel gradient grating period guided-mode resonance sensor array that is capable of simultaneously detecting multiple biomarkers with high sensitivity. A compact handheld reader was developed to acquire and analyze optical signals. By using creatinine and albumin as model biomarkers, we demonstrated that the developed platform could achieve sensitive, specific, and reproducible biomarker detection in both plasma and whole-blood samples. The platform can detect albumin and creatinine at concentrations as low as 0.8 and 1.44 μg/mL, respectively, and it exhibits minimal nonspecific binding. These results highlight the potential of the proposed system as a robust and accessible tool for decentralized diagnostics.

The ring-assisted Mach–Zehnder interferometer (RA-MZI) has high sensitivity and fast optical response time, and it has been used as a label-free nanophotonic biosensor. Most RA-MZI-based biosensors, however, require chemical modification of the ring surface to immobilize biomolecules that can interact with target molecules for sensing. Here, we report a novel microfluidic-integrated RA-MZI (μFRA-MZI) where a microfluidic channel was fabricated right above the photonic ring resonator. μFRA-MZI allows for direct sample delivery to the RA-MZI without chemical modification of the ring surface and measures shifts in the resonance wavelength induced by the presence of target molecules, enabling label-free detection. In order to optimize the sensitivity of μFRA-MZI, seven devices were fabricated with varied design parameters, including the gap distance between the ring and the bus waveguide (G_ring), the length of the multi-mode interferometer (L_MMI), and the length of the directional coupler (L_DC). Photonic characterization showed that the device with G_ring = 1.2 μm, L_MMI = 15.5 μm, and L_DC = 13.5 μm exhibited the highest extinction ratio (ER) compared to the other six devices, consistent with the simulation-optimized design. Testing with NaCl solutions of varying concentrations yielded a bulk sensitivity of 11.48 nm/refractive index unit (RIU) and an ER of 0.41. With the potential to further improve the device’s sensitivity and the ability to detect samples directly in flow without chemical modifications of the ring resonator, μFRA-MZI will provide a robust and effective approach for label-free biosensing.

Rapid, sensitive, and specific detection of pathogenic Escherichia coli serotypes is crucial for food safety and public health. Here, we present a surface-enhanced Raman scattering (SERS) platform utilizing highly ordered silver nanorod (AgNR) arrays functionalized with vancomycin for efficient and selective bacterial capture. The system enables multiplexed, high-throughput analysis using a portable Raman spectrometer, achieving direct molecular fingerprinting of seven clinically relevant E. coli serotypes. Systematic optimization of AgNR length and vancomycin coating maximized SERS enhancement and capture efficiency. Advanced data analysis with linear discriminant analysis (LDA) provided robust discrimination among all serotypes and concentrations, achieving up to 100% classification accuracy in single-concentration models and an overall accuracy of 98.41% when all concentrations and serotypes were evaluated jointly. This integrated SERS approach demonstrates significant promise for rapid, on-site bacterial diagnostics and quantitative pathogen monitoring, paving the way for practical applications in food safety and clinical microbiology.