Next Article in Journal
Study of New Therapeutic Strategies to Combat Breast Cancer Using Drug Combinations
Next Article in Special Issue
Total Fatty Acid Analysis of Human Blood Samples in One Minute by High-Resolution Mass Spectrometry
Previous Article in Journal / Special Issue
Recent Developments of Useful MALDI Matrices for the Mass Spectrometric Characterization of Lipids
Article Menu
Issue 4 (December) cover image

Export Article

Open AccessReview
Biomolecules 2018, 8(4), 174;

Quantification of Lipids: Model, Reality, and Compromise

Centre des Sciences du Goût et de l’Alimentation, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, 9E Boulevard Jeanne d’Arc, F-21000 Dijon, France
French LipidomYstes Network, 31000 Toulouse, France
Toxalim, Research Centre in Food Toxicology, Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, F-31027 Toulouse, France
Axiom Platform, MetaToul-MetaboHUB, National Infrastructure for Metabolomics and Fluxomics, F-31027 Toulouse, France
INTHERES, Université de Toulouse, INRA, ENVT, 31432 Toulouse, France
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS, INRA, CEA, 38000 Grenoble, France
MetaToul-Lipidomic Core Facility, MetaboHUB, I2MC U1048, Inserm, 31432 Toulouse, France
Author to whom correspondence should be addressed.
Received: 27 October 2018 / Revised: 30 November 2018 / Accepted: 4 December 2018 / Published: 14 December 2018
(This article belongs to the Special Issue Lipidomics)
Full-Text   |   PDF [298 KB, uploaded 14 December 2018]   |   Review Reports


Lipids are key molecules in various biological processes, thus their quantification is a crucial point in a lot of studies and should be taken into account in lipidomics development. This family is complex and presents a very large diversity of structures, so analyzing and quantifying all this diversity is a real challenge. In this review, the different techniques to analyze lipids will be presented: from nuclear magnetic resonance (NMR) to mass spectrometry (with and without chromatography) including universal detectors. First of all, the state of the art of quantification, with the definitions of terms and protocol standardization, will be presented with quantitative lipidomics in mind, and then technical considerations and limitations of analytical chemistry’s tools, such as NMR, mass spectrometry and universal detectors, will be discussed, particularly in terms of absolute quantification. View Full-Text
Keywords: lipidomic; mass spectrometry; nuclear magnetic resonance; quantification; universal detector lipidomic; mass spectrometry; nuclear magnetic resonance; quantification; universal detector
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Khoury, S.; Canlet, C.; Lacroix, M.Z.; Berdeaux, O.; Jouhet, J.; Bertrand-Michel, J. Quantification of Lipids: Model, Reality, and Compromise. Biomolecules 2018, 8, 174.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biomolecules EISSN 2218-273X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top