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Recent Developments of Useful MALDI Matrices for the Mass Spectrometric Characterization of Lipids

Quantification of Lipids: Model, Reality, and Compromise

Centre des Sciences du Goût et de l’Alimentation, AgroSup Dijon, CNRS, INRA, Université Bourgogne Franche-Comté, 9E Boulevard Jeanne d’Arc, F-21000 Dijon, France
French LipidomYstes Network, 31000 Toulouse, France
Toxalim, Research Centre in Food Toxicology, Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, F-31027 Toulouse, France
Axiom Platform, MetaToul-MetaboHUB, National Infrastructure for Metabolomics and Fluxomics, F-31027 Toulouse, France
INTHERES, Université de Toulouse, INRA, ENVT, 31432 Toulouse, France
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, CNRS, INRA, CEA, 38000 Grenoble, France
MetaToul-Lipidomic Core Facility, MetaboHUB, I2MC U1048, Inserm, 31432 Toulouse, France
Author to whom correspondence should be addressed.
Biomolecules 2018, 8(4), 174;
Received: 27 October 2018 / Revised: 30 November 2018 / Accepted: 4 December 2018 / Published: 14 December 2018
(This article belongs to the Special Issue Lipidomics)
Lipids are key molecules in various biological processes, thus their quantification is a crucial point in a lot of studies and should be taken into account in lipidomics development. This family is complex and presents a very large diversity of structures, so analyzing and quantifying all this diversity is a real challenge. In this review, the different techniques to analyze lipids will be presented: from nuclear magnetic resonance (NMR) to mass spectrometry (with and without chromatography) including universal detectors. First of all, the state of the art of quantification, with the definitions of terms and protocol standardization, will be presented with quantitative lipidomics in mind, and then technical considerations and limitations of analytical chemistry’s tools, such as NMR, mass spectrometry and universal detectors, will be discussed, particularly in terms of absolute quantification. View Full-Text
Keywords: lipidomic; mass spectrometry; nuclear magnetic resonance; quantification; universal detector lipidomic; mass spectrometry; nuclear magnetic resonance; quantification; universal detector
MDPI and ACS Style

Khoury, S.; Canlet, C.; Lacroix, M.Z.; Berdeaux, O.; Jouhet, J.; Bertrand-Michel, J. Quantification of Lipids: Model, Reality, and Compromise. Biomolecules 2018, 8, 174.

AMA Style

Khoury S, Canlet C, Lacroix MZ, Berdeaux O, Jouhet J, Bertrand-Michel J. Quantification of Lipids: Model, Reality, and Compromise. Biomolecules. 2018; 8(4):174.

Chicago/Turabian Style

Khoury, Spiro, Cécile Canlet, Marlène Z. Lacroix, Olivier Berdeaux, Juliette Jouhet, and Justine Bertrand-Michel. 2018. "Quantification of Lipids: Model, Reality, and Compromise" Biomolecules 8, no. 4: 174.

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