Microplastics

Microfibers are small fiber particles that range from 1 µm to 5 mm in length, generated through the home laundering and daily wear of textile garments. Microfibers stemming from synthetic textiles are a global pollution problem marked by their slow biodegradation and steady environmental accumulation. Thus, the quantification and study of factors controlling their generation is of interest. The aim of the current study included exploring the use of a Fiber Quality Analyzer-360 (FQA) for examining fiber counts and lengths of microfibers derived from cotton, flax, ramie, hemp, acrylic, polyester, viscose, and polyamide, and to explore if additional preparation steps, such as sonication, would improve microfiber detection by the system. While probe sonication led to higher fiber counts for most microfiber types, average microfiber lengths were statistically similar for most samples, with only the hemp and ramie samples showing statistically shorter microfibers following sonication. FQA detection estimates for cotton, viscose, and ramie microfibers were high, at 99, 101, and 116% for viscose, flax, and cotton, respectively. In contrast, synthetic microfibers of acrylic, polyamide and polyester showed 77, 43, and 14% detection rates, respectively. The high detection rate for the cotton sample is partly due to the higher fineness value obtained from the gravimetric determination. A similar calculation using AFIS fineness showed 86% detection. These observations confirm the significance of properly suspending the samples to accurately quantify microfibers while using the FQA system. Furthermore, the reduced detection of the examined synthetic microfibers suggests the limitations of the FQA as a technique for the direct comparison of natural and synthetic microfiber counts. Full article

Environmental contamination from mixtures of chemical substances and materials resulting from anthropogenic activities has adverse effects on aquatic biota. Fipronil (FP) is an insecticide widely used in agriculture and public health and is frequently detected in environmental compartments. Microplastics (MPs) represent another class of contaminants that have raised concerns due to their ability to adsorb pollutants, potentially acting as a vector. This study evaluated the influence of polyethylene microplastics on the toxicity of FP in zebrafish (Danio rerio) embryos/larvae. Zebrafish embryos were exposed to aqueous suspension containing purified FP and the FP + MP mixtures for 120 h. Mortality, teratogenicity and cardiotoxicity were observed during the zebrafish development. Larvae were more sensitive to FP than the embryo. The presence of MPs reduced lethality; however, no significant changes were observed for sublethal endpoints. Our findings demonstrate the acute impact of FP on zebrafish embryos/larvae, demonstrating that MPs interfere with toxicity. Therefore, understanding the mechanisms of interaction between contaminants of different classes is essential for predicting the associated risks to biota. Full article

Microplastic pollution, particularly particles smaller than 5 mm, poses significant environmental and health risks due to their potential for inhalation and long-range transport. This study provides the first long-term assessment of airborne microplastics and fibers in Porto, Portugal, over 18 months (September 2022 to March 2024). Bi-weekly samples were collected using a Microplastic Collector NILU, which were size-fractionated into five categories (>125 μm, 125–63 μm, 63–25 μm, 25–12 μm, and 12–1.2 μm) and quantified via optical microscopy. Microplastic concentrations ranged from 26 to 1484 MPs/day/m², while fiber concentrations varied from 14 to 646 fibers/day/m². With a focus on the 12–1.2 μm size range due to their classifications as PM₁₀ and PM_2.5, the highest microplastic concentrations were 164 MPs/day/m² (12–1.2 μm) and 534 MPs/day/m² (25–12 μm). Recovery rates varied among polymers, with PP, PE-HD, and ABS showing high accuracy (75.9%) and PES significantly lower (26.5%). This study highlights the significant temporal variability in airborne microplastic and fiber pollution, correlations with meteorological parameters, and the need for ongoing monitoring and targeted mitigation strategies to address associated health risks. Full article

Microplastics (MPs) are pervasive environmental contaminants in aquatic systems. Due to their small size, they can be ingested by aquatic biota, and numerous negative effects have been documented. Determining the risks to aquatic organisms is reliant on characterizing the environmental presence and concentrations of MPs, and developing efficient ways to do so over wide scales by means of aerial remote sensing would be beneficial. We conducted a systematic literature review to assess the state of the science of aerial remote sensing of aquatic MPs and propose further research steps to advance the field. Based on 28 key references, we outline three main approaches that currently remain largely experimental rather than operational: remote sensing of aquatic MPs based on (1) their spectral characteristics, (2) their reduction of water surface roughness, and (3) indirect proxies, notably other suspended water constituents. The first two approaches have the most potential for wide-scale monitoring, and the spectral detection of aquatic MPs is seemingly the most direct approach, with the fewest potential confounding factors. Whereas efforts to date have focused on inherently challenging detection in coarse-resolution satellite imagery, we suggest that better progress could be made by experimenting with image acquisition at much lower altitudes and finer spatial and spectral resolutions, which can be conveniently achieved using drones equipped with high-precision hyperspectral sensors. Beyond developing drone-based aquatic MP monitoring capabilities, such experiments could help with upscaling to satellite-based monitoring for global coverage. Full article

The threat of plastic pollution has escalated to unprecedented levels, with particular concern surrounding microplastics (MPs) and artificial fibers or particles (AFs) due to their wide distribution across ecosystems and their bioavailability to wildlife. Although research on the impact of plastic on wild birds is rapidly growing, knowledge of terrestrial species remains limited, especially regarding raptors, which have been significantly understudied. Here, we investigated the prevalence of MPs and AFs in regurgitated pellets from six protected terrestrial raptor species, namely the Cinereous Vulture (Aegypius monachus), the Bonelli’s Eagle (Aquila fasciata), the Little Owl (Athene noctua), the Lesser Kestrel (Falco naumanni), the Red Kite (Milvus milvus), and the Barn Owl (Tyto alba), collected between 2022 and 2023. Our analysis revealed that 68% of the pellets contained MPs (47 out of 69), and 81% contained AFs (56 out of 69). Additionally, two macroplastics were found inside the pellets: a cable tie in a Red Kite and a bird identification ring in a Cinereous Vulture. The concentrations (mean ± standard error of the mean) were 2.39 ± 0.39 MPs/pellet and 5.16 ± 0.72 AFs/pellet. The concentration of MPs and AFs varied significantly among some of the studied species; however, no significant differences were observed among urban, rural, and protected areas. This could indicate that contamination levels are mainly related to the type of species. Fibers emerged as the predominant contaminant shape, with six different polymers identified, among which PET, PE, and acrylics were the most prevalent. These findings highlight that plastic pollution has reached protected terrestrial raptors and that the impact of plastic on their life cycles needs to be assessed. Full article

Microplastics (MPs) are omnipresent particles that receive special attention because of their persistent nature and their potential impact on human disease and on the environment. Most MPs are generated by the degradation of larger plastic items such as clothing, car tires, and discarded plastic materials. In indoor environments, where human beings spend most of their time, aerial MP levels are higher, and the majority are fibers produced from textiles. Airborne MPs indoors are a greater potential danger to humans than MPs ingested in food and drink. Fragments small enough to remain substantially suspended in the air column, the small airborne microparticles that are measured as PM₁₀ and PM_2.5, become available for assimilation by human beings through respiration, potentially producing various health problems. Larger MPs act by ingestion and skin contact. MPs can carry microorganisms and micropollutants adsorbed to their surfaces, facilitating their uptake and survival within the human body. Indoor airborne MPs thus represent emerging pollutants of fast-growing concern that are especially important as potential invaders of the human respiratory system, reaching the alveoli of the lungs and finally entering the circulatory system and other tissues. Since this direct human exposure to MP contamination via indoor air is so important, we discuss in this article the ways in which MP concentration and dispersal in indoor air can be affected by air turbulence that is induced by anthropogenic objects such as air conditioners, filters, and purifiers. Much evidence is equivocal and further research is necessary. Full article

Microplastics (MPs) are persistent, globally relevant pollutants that have thus far been rigorously studied in natural waters but have not been as extensively studied in industrial wastewaters. Samples were collected from the forestry industry, wastewater treatment plants and the biogas industry. An enzymatic treatment protocol for MPs’ detection was applied to an assortment of industrial samples ranging from wastewaters, effluents and condensates to sludges and digestates. The effects of selected enzymes were studied systematically to develop a basis for digestion protocols on industrial samples. Further, different methods of detection (micro FTIR and Raman) were compared to each other, and the samples were visually examined using SEM. The developed protocols in this study were then compared with blank samples, contamination controls and samples spiked with artificial microplastics. This research aimed to fill some of the gap in the knowledge regarding the analysis methods and especially in the type of samples screened for microplastics thus far and presents a systematic approach to MPs’ detection in industrial wastewaters. It highlights the issues with the used analytical methods (such as misidentification) and validates the analysis results with milled, random shape and wide-size-range reference MPs that represent real samples better than standardized, ideal round beads. This study provides the first-ever suggestion for an enzymatic digestion protocol for industrial sample analysis. Full article

Plastic and microplastic contamination continue to be growing problems across the globe for both ecosystems and human health. Canada has banned single-use plastic products such as bags, cutlery, and foodservice ware (containers) to address and mitigate plastics and microplastic contamination. This study evaluates the life cycle of banned plastic products and their alternatives to determine whether environmental impacts can be mitigated. The environmental impacts of bags (plastic, paper, cotton), cutlery (plastic, wooden, biodegradable), and containers (plastic, styrofoam, biodegradable) were determined considering both domestic and imported products. The bag study saw paper bags having the highest environmental impacts and cotton bags with the lowest due to their reusability. For the cutlery study, plastic cutlery was the most impactful across all categories except for eutrophication and ozone depletion, where biodegradable cutlery was the most impactful by 25% and 35%, respectively. In the case of foodservice ware (containers), styrofoam was found to be the least impactful. Similar to cutlery, the plastic containers had the greatest impact except where the biodegradable container contributed more to ozone depletion and eutrophication by 25% and 45%, respectively. Local production reduced impacts across all categories. Furthermore, on a local scale, biodegradable cutlery had a greater impact on the smog and respiratory effects categories than plastic by 10% and 30%, respectively. The results of this study indicate that future regulations should focus on promoting and educating consumers on the use of reusable products over single-use products, funding research to mitigate challenges associated with waste management, and consider an informed ban on all single-use products and not just those made of plastic material to mitigate environmental impacts. Full article

Synthetic fibers are widely used in daily life due to their durability, elasticity, low cost, and ease of use. The textile industry is the primary source of synthetic microfibers, as these materials are mostly used in production processes. Globally, plastic pollution has been identified as a major environmental threat in this era, since plastics are not degradable but break down into smaller particles such as mesoplastics, microplastics, and microfibers. Synthetic microfiber pollution is a significant issue in aquatic ecosystems, including oceans and rivers, with laundry wastewater being a major source. This problem is particularly pressing in cities like Galle, Sri Lanka, where numerous tourist hotels are located. Despite the urgency, there has been a lack of scientific and systematic analysis to fully understand the extent of the issue. This study addresses this gap by analyzing the generation of microfibers from laundry activities at a selected hotel and evaluating the efficiency of a laundry wastewater filtration system. This study focused on a fully automatic front-loading washing machine (23 kg capacity) with a load of 12 kg of polyester–cotton blend serviettes (black and red). Samples (1 L each) were taken from both treated and untreated wastewater during four wash cycles, with a total of 100 L of water used for the process. The samples were filtered through a 100 μm sieve and catalytic wet oxidation along with density separation were employed to extract the microfibers, which were then collected on a membrane filter paper (0.45 μm). Microfibers were observed and analyzed for shapes, colors and sizes under a stereo microscope. Results revealed that untreated laundry wastewater contained 10,028.7 ± 1420.8 microfibers per liter (n = 4), while treated wastewater samples recorded 191.5 ± 109.4 microfibers per liter (n = 4). Most of the microfibers observed were black and white/transparent colors. Further analysis revealed that 1 kg of polyester–cotton blend fabric can generate 336,833 microfibers per wash, which was reduced to 6367 microfibers after treatment. The filtration unit recorded an impressive efficiency of 98.09%, indicating a remarkably high capacity for removing microfibers from wastewater. These findings highlight the potential of such filtration techniques to significantly reduce microfiber emissions from laundry wastewater, presenting a promising approach to mitigating environmental pollution from microfibers. Full article

Microplastics, plastic fragments smaller than 5 mm present in the environment due to the decomposition of larger plastics, can cause damage to various ecosystems and species of pollinating insects, such as Apis mellifera bees. These bees play a crucial role in the ecology and production of honey and pollen, also serving as bioindicators of environmental quality as they are sensitive to contaminants such as microplastics. In this study, we evaluated the presence of microplastics in these insects and their products—pollen, and honey—collected in August 2021 and August 2023 in rural areas (Tabio and Guasca) of Cundinamarca, Colombia, and urban areas (Universidad Nacional de Colombia and Pontificia Universidad Javeriana) of Bogotá, Colombia. Each year, 24 bees, 10 g of honey, and 5 g of pollen were collected per sampling point. Microplastics in bees and their products were identified and quantified by stereomicroscopy, with or without hydrogen peroxide digestion pretreatment. Microplastics were found in bees, pollen, and honey in both periods, with an increase in their quantity observed over time due to increasing environmental pollution. Blue fibers were the most common microplastics, with a greater amount recorded in 2023 compared to 2021. Full article

Environmental pollution from plastics has become one of the biggest concerns globally. Microplastics (MPs) are plastic materials less than 5 mm in size. They remain in the environment for hundreds to thousands of years without degrading, only breaking down further to nanoplastics (NPs). Micro- and nanoplastics can be the origin of many diseases and can carry various pathogenic substances on their surface and spread them throughout the biosphere, starting with contained additives and ending with adsorbed toxins from the environment and potentially pathogenic microorganisms. Exposure routes for humans and animals are through air, water and food/feed. Due to the placement of livestock—including ruminants, fish and poultry—and humans at the top of the food web, any pollution in water, air or soil can eventually be transferred to livestock and from livestock to humans. The presence of microplastics in the intestines of aquaculture species, ruminants and poultry, for instance, was found to cause a change in the intestinal microbial population and, as a result, the occurrence of diseases. These particles have also been observed in other organs such as liver, kidneys, lung, spleen, heart, ovaries, and testicles of animals, which causes biochemical changes, structural destruction, and malfunction. While the complete extent of the negative health impacts of microplastics remains still largely unknown, their ubiquitous presence and the transmission of chemicals from microplastics to organisms is a notable issue, underscoring the importance of gaining a more comprehensive understanding of the potential threats posed by microplastics to animal and ultimately human health, coupled with a need for drastic reduction of the plastic freight into the environment. This review article summarizes recent findings on the effect of micro- and nanoplastics on farmed animals and, ultimately, on humans. Action is needed to reduce the number of microplastics to which farmed animals, and thereby humans, are exposed. Full article

Plastic is a growing global environmental problem. While much of the focus of anthropogenic microparticles has focused on microplastics and their occurrence in marine systems, anthropogenic microparticles are found in freshwater systems. The Charles River is a highly impacted and historically important river within Massachusetts and runs for 80 miles within the state of MA through a variety of land uses. Microparticle concentrations were found to vary along the length of the river and ranged in concentrations from 1–19 pieces/L, with generally higher concentrations downstream. Microfibers were the dominant (72%) type of microparticles found, and the majority (avg 76%) of microparticles were synthetic. The highest estimated flux of microparticles occurred in May, with an estimated flux of 2 billion microparticles per day via the Charles River into the Boston Harbor. The average annual concentration of microparticles was correlated with land use, with higher concentrations occurring in regions with higher impervious coverage and in areas designated as industrial or high-density residential. Polyester, polypropylene, and polyamides were the dominant plastic polymers. However, seasonal changes in the relative importance of each polymer, along with changes in the abundance and flux rates, indicate that there would be seasonal variability in the type of microparticles exported. Changes in composition occurred between stations and between the head and mouth of the river, suggesting particle retention due to either deposition, degradation, or biological consumption. Full article

Concern over microplastics (MPs) in the environment is rising. Microplastics are generally known to exist in aquatic settings, but less is known about their occurrence in soil ecosystems. When plastic waste builds up in agricultural areas, it can have a negative impact on the environment and food sources, as well as have an indirect effect on all trophic levels of the food chain. This paper addresses the relationship between microplastics and the management of plastic waste, which contributes to their accumulation, and it describes the sources and the movement processes of microplastics in agricultural soils as a result of natural events and disasters. Evaluating the impact of weather on coastal microplastic contamination is critical, as extreme weather events have become more frequent in recent years. This study sheds light on how weather patterns affect the dispersion of plastic waste in terrestrial habitats, including the impacts of seasonality and extreme weather. According to the results of this review, typhoons, monsoons, rainfall, and floods contribute significantly more microplastics to the surface sediment through surface runoff and wind transport, particle redistribution caused by agitated waves, and fragmentation under intense abrasion forces. Severe weather conditions have the potential to disperse larger and more varied kinds of microplastics. Full article

An experimental study was conducted to identify the behaviour of neutrally buoyant microplastics (specific density, 0.94) in different hydrodynamic conditions while focusing on combined wave–current conditions and the mixing across the hyporheic zone. For in-water-column microplastics, it was observed that the streamwise dispersion of neutrally buoyant microplastics is comparable to solute dye in both slow open-channel flow conditions and combined wave–current conditions. However, for in-bed microplastics, when compared to soluble tracers, the longer timespans associated with the hyporheic exchange process allowed the density effects to enhance the vertical exchange when compared to solutes. Full article

Wastewater treatment plants (WWTPs) have been identified as important point sources for microplastics (MPs) in the environment; monitoring MP emissions in the WWTP effluent is therefore essential for contamination control. The aim of this study is to acquire a large number of samples (320) over a period of two years and three months to determine the temporal variations in microplastic contamination in the outlet of the municipal WWTP Landau-Mörlheim. The effluent of the third cleaning stage is sampled with a 10 µm filter cartridge, processed in the laboratory using a hydrogen peroxide treatment, and MPs are then detected by fluorescence staining. The results show high temporal variations in the microplastic concentrations in the effluent of the WWTP. This indicates that high numbers of samples are necessary to obtain a representative assessment of the microplastic emissions; single samples are not representative. The average microplastic concentration in the effluent was 27.8 ± 29.8 MP/L, ranging from 0.6 MP/L to 194.0 MP/L. This leads to a yearly emission of 1.5 × 10¹¹ MP for the WWTP Landau-Mörlheim, corresponding to an emission of 2.8 × 10⁶ MP/inhabitant and year. Statistically significant seasonal variations could not be observed, although there is a trend towards lower MP concentrations in summer. Further, no correlations with other wastewater or weather parameters could be found. Full article

Plastic pollution has become a growing concern for environmental and human health in recent years. Currently, research suggests that soil compartments might be highly contaminated, with compost being one of the major sources for plastic contamination, especially at reduced sizes. In Geneva, microplastic contamination has already been evaluated in industrial compost but not yet in field-side compost. Therefore, this work focuses on the evaluation of concentrations and the characterization of microplastic particles (MPs) in field-side compost in Geneva. Four different field-side composts were sampled in Geneva and sieved with sizes ranging from >5 mm to 0.5 mm. The MPs were visually sorted when possible, and the smaller ones were treated by digestion and density separation processes. All of the MPs were analyzed with infrared spectroscopy. The results (sizes > 5 mm to 1.25 mm) reveal concentrations between 195 ± 64 and 1315 ± 375 plastic particles/kg of compost and the presence of conventional plastics in every compost. MPs were present in almost every size fraction for the four composts, and the origin of waste seemed to influence their concentrations. Indeed, composts free from organic household waste had lower concentrations than the ones accepting that kind of waste. Littering and waste still wrapped in plastic were also noticed for the composts with higher concentrations of MPs. The characterization results highlight the variety of plastic types among the composts, mostly represented by PE (polyethylene), PVA (polyvinyl alcohol), PP (polypropylene), and DAIP (polydiallyl iso-phthalate) in general but also specific contamination by PEMA (poly ethyl methacrylate) for only one of the composts. Further work is needed to evaluate the distribution of MPs according to their size or the types of waste used in composting, especially the potential degrading conditions of plastics, to mitigate their presence in composts. Full article

Under varying flow rate conditions, the transport and retention of polydisperse microplastics (MPls), with an average particle size of 16 ± 6 µm, were investigated in saturated porous media. First-order reversible and irreversible kinetic sorption models were used to describe the sorption kinetics. Sensitivity analyses provided insight into the effects of each sorption parameter. Both numerical modeling and experimental measurements were utilized to evaluate the retention rates of sand filters. The influence of flow rate on sorption was reflected in variations in the distribution coefficient (K_d), the mass transfer coefficient (β), and the irreversible sorption rate (K₁). Lower flow rates were associated with higher K_d and β values, indicating increased sorption and reduced mass transfer rates. An increase in K_d resulted in a more gradual sorption process, with a decrease in peak concentration, whereas changes in β had a comparatively smaller impact on sorption rate and peak concentration. Lower K₁ values were linked to higher peak concentrations and decreased retention efficiency. Numerical modeling revealed retention rates of 28 ± 1% at a flow rate of 31 mL min⁻¹ and 17 ± 1% at 65 mL min⁻¹. The introduction of MPls into saturated sand environments modifies the transport dynamics within the medium. Consequently, these alterations affect the hydrological characteristics of porous media, impacting groundwater quality and agricultural output. The mean absolute error (MAE) of 6% between the modeled and observed retention rates indicated a high level of accuracy. This study underscores the importance of examining retention efficiency and the accuracy of numerical models in understanding MPl transport in porous media. Full article

Microplastics have emerged as ubiquitous contaminants, attracting increasing global attention. Recent evidence confirms the presence of microplastics in human blood, suggesting their potential to interact with cells and induce adverse physiological reactions in various organs as blood circulates. To quantify the distribution of microplastics and assess their potential effects on human health, the effective separation of microplastics from blood is crucial. However, current methods for separating microplastics from blood are limited in effectiveness and simplicity. This study proposes a microfluidic device that utilizes traveling surface acoustic waves to separate microplastics from blood. While traveling surface acoustic waves have been employed to separate various particles, a systematic study on the separation of microplastics from blood samples has not been previously reported. Specifically, the theoretical values of the acoustic radiation factor for various types of microplastics and blood cells were investigated. The significant differences in resonant frequencies indicated the feasibility of separating microplastics of different sizes and types from blood cells. Experimental validation was performed using a polydimethylsiloxane microfluidic device on a piezoelectric lithium niobate substrate. The device successfully separated 5- and 10-micrometer polystyrene microplastics from blood samples. The effects of power and flow rate on separation efficiency were also systematically investigated. This study provides a novel approach for the effective separation of microplastics from blood, contributing to the assessment of their distribution and potential health impacts. Full article

While there are a number of available reference and testing materials for micro- and nanoplastic (MNP) studies in toxicology, they are not well-characterized and do not cover all major polymer types that may potentially pollute the environment. This review article will address the question of why we need properly designed synthesized micro- and nanoplastics with a core–shell structure (with organic–inorganic units) and provide researchers with a scientific basis for the design of synthesized MNP particles. It will include a list of commercially available MNPs, an overview of the theoretical background to polymer particle synthesis, and an analysis of the advantages and disadvantages of MNP preparation methods, namely, fragmentation and synthesis, along with examples of synthesized MNP particles. The current study will demonstrate that polystyrene is one of the most prevalent MNP particle types among reference materials from certification bodies and among testing particles synthesized by chemical scientists. Nevertheless, the global industrial production of polystyrene represents approximately 5% of the total, and it is not a dominant plastic type in the textile or packaging industries. In contrast to mechanically fragmented MNP particles, the synthesis approach offers the potential to control the physico-chemical properties, enabling the more selective detection and quantification, as well as a greater comparability of the results amongst toxicological studies. Full article