A new crAss-like genome encoding diversity-generating retroelement (DGR) was found in the fecal virome of a healthy volunteer. The genome of the phage referred to as the crAssphage LMMB, belonged to the candidate genus I of the AlphacrAssvirinae subf...
Obtaining a full short tandem repeat (STR) profile from a low template DNA (LT-DNA) still presents a challenge for conventional methods due to significant stochastic effects and polymerase slippage. A novel amplification method with a lower cost and...
The Corinth Rift, in Central Greece, is one of the most seismically active areas in Europe. In the eastern part of the Gulf of Corinth, which has been the site of numerous large and destructive earthquakes in both historic and modern times, a pronoun...
When analyzing challenging samples, such as low-template DNA, analysts aim to maximize information while minimizing noise, often by adjusting the analytical threshold (AT) for optimal results. A potential approach involves calculating the AT based on...
The effective detection of repeated patterns from inputs of unknown fronto-parallel images is an important computer vision task that supports many real-world applications, such as image retrieval, synthesis, and texture analysis. A repeated pattern i...
This work describes an intriguing strategy for the creation of arbitrarily shaped hydrogels utilizing a self-healing template (SHT). A SHT was loaded with a photo-crosslinkable monomer, PEG diacrylate (PEGDA), and then ultraviolet light (UV) crosslin...
This paper presents a novel method to extract local features, which instead of calculating local extrema computes global maxima in a discretized scale-space representation. To avoid interpolating scales on few data points and to achieve perfect rotat...
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas gene editing systems have enabled molecular geneticists to manipulate prokaryotic and eukaryotic genomes with greater efficiency and precision. CRISPR/Cas provides adaptive immuni...
By using ginkgo leaves (GL) as template and Zn(CH3COO)2∙2H2O as Zn source, a series of ZnO samples with special morphology were prepared via a template-assisted two-steps method without adding any other additives. The degradation of the dye MB was us...
A pilot study employing a template-matching approach on the detection of repeating earthquakes (or repeaters) in a small volume of the Irpinia fault (Southern Italy) is presented here. A catalog of repeaters nucleating over about a decade with local...
A hexanucleotide repeat expansion in C9ORF72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Bidirectional transcription of the repeat expansion generates sense and antisense RNAs that are tr...
Due to differences in synthetic aperture radar (SAR) and optical imaging modes, there is a considerable degree of nonlinear intensity difference (NID) and geometric difference between the two images. The SAR image is also accompanied by strong multip...
A composite based on amino-containing humic acid with the immobilization of multi-walled carbon nanotubes preliminarily tuned to a copper ion has been obtained. The synthesis of a composite pre-tuned for sorption by the local arrangement of macromole...
Multichannel graphite electrodes (MGrEs) have been designed and fabricated in this study. A template was cut from an adhesive plastic sheet using a desktop cutting device. The template was placed on a polypropylene substrate, and carbon graphite ink...
Protecting target regions from reconnaissance is a critical task in synthetic aperture radar (SAR) countermeasures. For large facilities such as airports and key infrastructure, deceptive scene jamming requires more than inserting isolated false targ...
Telomerases are moderately processive reverse transcriptases that use an integral RNA template to extend the 3′ end of linear chromosomes. Processivity values, defined as the probability of extension rather than dissociation, range from about 0...
Tuberculosis (TB), a disease caused by Mycobacterium tuberculosis (MTB) infection, remains a major threat to global public health. To facilitate early TB diagnosis, an IS6110 gene-based recombinase aided amplification (RAA) assay was coupled to a clu...
The estimation of the Instantaneous Angular Speed (IAS) has in recent years attracted a growing interest in the diagnostics of rotating machines. Measurement of the IAS can be used as a source of information of the machine condition per se, or for pe...
Humidity-sensitive poly(ethylene glycol) (PEG) inverse opals with micropatterns of 2 μm wide anti-swell-broken grooves were prepared using polystyrene (PS) colloidal crystals as templates and colloidal lithography. Monodisperse PS colloids were depos...
In this work, a simple and label-free fluorescence “off” to “on” platform was designed for the sensitive and selective detection of microRNA (miRNA) in cancer cells. This method utilized a padlock DNA-based rolling circle ampl...
A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is...
Recent advances in nanoscience have opened ways of recycling substrates for nanomaterial growth. Novel materials, such as atomically thin materials, are highly desirable for the recycling substrates. In this work, we report recycling of monolayer gra...
Surface-enhanced Raman spectroscopy (SERS) enables ultra-sensitive molecular detection and has broad analytical and biomedical applications; recent advances focus on high-performance substrates and innovative detection strategies. However, achieving...
In the existing cancellable finger vein template protection schemes, the original biometric features cannot be well protected, which results in poor security. In addition, the performance of matching recognition performances after generating a cancel...
Validated assessment protocols have been developed to quantify welfare states for intensively managed sport, pleasure, and working horses. There are few protocols for extensively managed or free-roaming populations. Here, we trialed welfare indicator...
Silicon/carbon (Si/C) composites combine the high theoretical specific capacity of silicon with the electronic conductivity, structural stability, and volume-buffering capability of carbon, making them promising anode candidates for next-generation h...
Guanine-rich DNA can fold into highly stable four-stranded DNA structures called G-quadruplexes (G4). Originally identified in sequences from telomeres and oncogene promoters, they can alter DNA metabolism. Indeed, G4-forming sequences represent obst...
The human telomere region is known to contain guanine-rich repeats and form a guanine-quadruplex (G4) structure. As telomeres play a role in the regulation of cancer progression, ligands that specifically bind and stabilize G4 have potential therapeu...
A new method for the recognition of meaningful changes in social state based on transformations of the linguistic content in Arabic newspapers is suggested. The detected alterations of the linguistic material in Arabic newspapers play an indicator ro...
We developed a novel electrochemical sensor for the detection of alfuzosin (AFZ), a drug used to treat benign prostatic hyperplasia, using a double-shelled Co3O4/NiCo2O4 nanocomposite-modified electrode. The nanocomposites were synthesized using a te...
Alumina supports presenting a bimodal porosity are generally advantageous for the conversion of bulky molecules such as found in biomass, refining, and petrochemistry. However, shaping of such materials, while controlling pores size and orientation,...
Achieving stable local focus-height measurement across different material surfaces is important for I-line-lithography-related inspection, where sub-micrometer height deviations can affect imaging quality, exposure uniformity, and subsequent autofocu...
Micropatterning technology offers powerful methods for biological analyses at the molecular level, enabling the investigation of cell heterogeneities, as well as high throughput detection. We herein propose an approach for single-cell patterning. The...
We present here a method for recognising dance moves in sequences using 3D accelerometer and gyroscope signals, acquired by a single wearable device, attached to the dancer’s leg. The recognition entails dance tempo estimation, temporal scaling...
Security design for large-scale infrastructure systems requires substantial effort and often causes development delays. In line with NIST guidance, such systems should consider security design throughout a system development lifecycle. Nevertheless,...
This study proposes a microfluidic spinning method to form alginate microfibers with branched and chained structures by controlling two streams of a sodium alginate solution extruded from a theta-glass capillary (a double-compartmented glass capillar...
String edit distances have been used for decades in applications ranging from spelling correction and web search suggestions to DNA analysis. Most string edit distances are variations of the Levenshtein distance and consider only single-character edi...
The application of next generation sequencing (NGS) for the analysis of mitochondrial (mt) DNA, short tandem repeats (STRs), and single nucleotide polymorphism (SNPs) has demonstrated great promise for challenging forensic specimens, such as degraded...
Pd-functionalized one-dimensional (1D) SnO2 nanostructures were synthesized via a facile hydrothermal method and shaddock peels were used as bio-templates to induce a 1D-fiber-like morphology into the gas sensing materials. The gas-sensing performanc...
Inverted repeat (IR) DNA sequences compose cruciform structures. Some genetic disorders are the result of genome inversion or translocation by cruciform DNA structures. The present study examined whether exogenous DNA integration into the chromosomes...
Certain G-rich DNA repeats can form quadruplex in bacterial chromatin that can present blocks to DNA replication and, if not properly resolved, may lead to mutations. To understand the participation of quadruplex DNA in genomic instability in Escheri...
Achieving high-precision overlay target center localization is critical for image-based overlay (IBO) metrology in advanced semiconductor manufacturing. This paper proposes a novel IBO target localization algorithm based on symmetry center matching....
In this paper, an automatic approach for zebra crossing extraction and reconstruction from high-resolution aerial images is proposed. In the extraction procedure, zebra crossings are extracted by the JointBoost classifier based on GLCM (Gray Level Co...
The increasing penetration of renewable generation and power-electronic-interfaced devices changes the sources of inertia, frequency-control resources, and structural sensitivity of power-system frequency responses. This paper develops a traceable au...
Pipecolic acid (PA) is an important biomarker associated with peroxisomal and neurological disorders, necessitating the development of rapid, selective, and cost-effective detection methods beyond conventional chromatographic techniques. In this stud...
Droplet digital polymerase chain reaction (ddPCR) is a method used to detect and quantify nucleic acids even when present in exceptionally low numbers. While it has proven to be valuable for clinical studies, it has failed to be widely adopted for en...
Functional, comfortable prosthetic limbs depend on personalised sockets, currently designed using an iterative, expert-led process, which can be expensive and inconvenient. Computer-aided design and manufacturing (CAD/CAM) offers enhanced repeatabili...
Sample entropy, an approximation of the Kolmogorov entropy, was proposed to characterize complexity of a time series, which is essentially defined as
DNA and RNA nucleotide sequences are ubiquitous in all biological cells, serving as both a comprehensive library of capabilities for the cells and as an impressive regulatory system to control cellular function. The multi-alignment framework (MAF) pr...
Microscopic visual measurement is one of the main methods used for precision measurements. The observation morphology and image registration algorithm used in the measurement directly affect the accuracy and speed of the measurement. This paper analy...
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