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First crAss-Like Phage Genome Encoding the Diversity-Generating Retroelement (DGR)

Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia
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These authors contributed equally to this work.
Viruses 2020, 12(5), 573; https://doi.org/10.3390/v12050573
Received: 10 April 2020 / Revised: 15 May 2020 / Accepted: 19 May 2020 / Published: 22 May 2020
(This article belongs to the Section Bacterial Viruses)
A new crAss-like genome encoding diversity-generating retroelement (DGR) was found in the fecal virome of a healthy volunteer. The genome of the phage referred to as the crAssphage LMMB, belonged to the candidate genus I of the AlphacrAssvirinae subfamily. The DGR-cassette of the crAssphage LMMB contained all the essential elements: the gene encoding reverse transcriptase (RT), the target gene (TG) encoding the tail-collar fiber protein, and variable and template repeats (VR and TR) with IMH (initiation of mutagenic homing) and IMH* sequences at the 3′-end of the VR and TR, respectively. Architecture of the DGR-cassette was TG-VR(IMH)-TR(IMH*)-RT and an accessory variable determinant (avd) was absent from the cassette. Analysis of 91 genomes and genome fragments from genus I of the AlphacrAssvirinae showed that 15 (16%) of the genomes had DGRs with the same architecture as the crAssphage LMMB, while 66 of the genomes contained incomplete DGR-cassettes or some elements of the DGR. View Full-Text
Keywords: crAssphage; genome; diversity-generating retroelement (DGR); variable repeat; template repeat; initiation of mutagenic homing crAssphage; genome; diversity-generating retroelement (DGR); variable repeat; template repeat; initiation of mutagenic homing
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Morozova, V.; Fofanov, M.; Tikunova, N.; Babkin, I.; Morozov, V.V.; Tikunov, A. First crAss-Like Phage Genome Encoding the Diversity-Generating Retroelement (DGR). Viruses 2020, 12, 573.

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