Selective staining of extracellular vesicles (EVs) is a major challenge for diagnostic and therapeutic applications. Herein, the EV labeling properties of a new class of tetranuclear polypyridylruthenium(II) complexes, Rubb7-TNL and Rubb7-TL, as phos...
The identification of protein aggregates as biomarkers for neurodegeneration is an area of interest for disease diagnosis and treatment development. In this work, we present novel super luminescent conjugated polyelectrolyte molecules as ex vivo sens...
Heat-tolerant species have become increasingly important because of climate change; however, a selection system for woody plants is not well established. This study was conducted to establish a selection system for heat-tolerant woody plant species a...
Due to climate change, heat-tolerant species have become increasingly important; however, the selection system for herbaceous plants has yet to be well established. This study aimed to establish a selection system for heat-tolerant plant species and...
Background and objective: The aim of this randomized split-mouth study-controlled clinical trial was to compare the 2-year clinical performance of resin composite restorations placed at non-caries cervical lesions (NCCL) with one-step self-etch, tota...
This multi-center study evaluated a novel microscope system capable of quantitative phase microscopy (QPM) for label-free sperm-cell selection for intracytoplasmic sperm injection (ICSI). Seventy-three patients were enrolled in four in vitro fertiliz...
In domestic cats, the maturation, fertilization, and development potential in vitro decreases during the non-breeding season. This study aims at evaluating the efficacy of Brilliant Cresyl Blue (BCB) staining in selecting developmentally competent oo...
Stains, as one of most common degradations of paper cultural relics, not only affect paintings’ appearance, but sometimes even cover the text, patterns, and colors contained in the relics. Virtual restorations based on common red–green&nd...
The European spruce bark beetle, Ips typographus (L.), is a major pest of Norway spruce. During outbreaks, the beetles can colonize moderately stressed trees via mass attacks mediated by aggregation pheromones, while at endemic population levels, bee...
The present study aims to discover novel derivatives as antiapoptotic agents and their protective effects against renal ischemia/reperfusion. Therefore, a series of new thiadiazole analogues 2a–g was designed and synthesized through cyclization...
Microplastics are increasingly suspected of having serious negative effects on ecological systems and living organisms. These effects are different based on the materials of the microplastics, leading to the importance of the determination of the mat...
Mediterranean pine engraver, Orthotomicus erosus was never considered as a significant pest in Croatia and did not appear in high population densities until 2017, when it reached outbreak level in Aleppo pine stands. The beetle was first detected in...
Although plastics have benefited our lives in terms of cost and convenience, the disposal of end-of-life plastics poses environmental problems, such as microplastics (MPs). Although the separation (e.g., filtration) and staining of MPs with fluoresce...
(1) Background: The aim of the paper is to determine how selected staining liquids affect color stability and reflectance of six composite and compomer dental materials. (2) Methods: Six dental materials were chosen for this study: G-aenial (GC), Fil...
Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue sections. Here, we describe an effective method f...
The complex chemical composition of certain color stains on textiles requires an optimal proportion of thermal and chemical action in the Sinner cycle of the washing process. In this study, both factors were analyzed by varying the composition of the...
Pre-wash sorting of medical textiles is essential for hospital infection control, yet accurate stain detection remains challenging because visually apparent stains often have blurred boundaries, whereas dried urine stains lack distinguishable optical...
Background: Micro- and macroabrasion represent a minimally invasive treatment approach for stained teeth in the esthetic zone. Diagnosing the type of stain is crucial for selecting the appropriate treatment approach. These clinical procedures involve...
The presence of lymphovascular invasion (LVI) in urothelial carcinoma (UC) is a poor prognostic finding. This is difficult to identify on routine hematoxylin–eosin (H&E)-stained slides, but considering the costs and time required for examin...
Existing nuclei segmentation methods have obtained limited results with multi-center and multi-organ whole-slide images (WSIs) due to the use of different stains, scanners, overlapping, clumped nuclei, and the ambiguous boundary between adjacent cell...
This article presents the operation of an automatic pine sawn timber inspection system, which was developed at the Woodinspector company and is offered commercially. The vision inspection system is used to detect various wood defects, including knots...
Background: Endoscopic ultrasound-guided puncture aspiration biopsy (EUS-FNA) of pancreatic ductal adenocarcinoma (PDAC) is highly diagnostic, but it is difficult to distinguish from benign disease. Our objective was to determine the usefulness of ma...
This study evaluated cold tolerance and selected superior individuals from 100 Zoysia japonica ‘Zenith’ plants. Following pre-cold treatment at 4 °C for 24 h, candidates were screened using electrolyte leakage (EL) with a 10% selectio...
Background: Digitally stained ex vivo confocal laser scanning microscopy (CLSM) scans are a possible alternative to formalin-fixed and paraffin-embedded (FFPE) and hematoxylin-eosin (H&E) stained slides. This study explores the diagnostic accurac...
Background and Objectives: The worldwide incidence of renal cell carcinoma (RCC) rose by 22% between 2012 and 2022. In Australia, RCC accounted for 2.8% of all cancer diagnoses and contributing to 1.8% of cancer-related deaths. Identification of RCC...
Background/Objectives: Despite hematoxylin and eosin (H&E) staining remaining the cornerstone of histopathological diagnosis, substantial intra- and inter-laboratory variability persists. This issue is increasingly relevant in Digital Pathology,...
In this work, the creation of a dataset labeled in a pixel-wise manner for the uncommon domain of stain detection on patterned laundry is described. The unique properties of images in this dataset—stains are small and sometimes occur in large amounts...
The withdrawal of the iView detection system (iV) forced many cytopathology laboratories, including ours, to substitute immunocytochemical (ICC) staining protocols for routine practice with other detection systems. Our objective was to optimize, vali...
Wood staining is a crucial technique for enhancing the decorative effect of wood. Different mordants and mordant processes can influence the staining effect of wood. In this study, three types of mordants and mordant methods were selected to improve...
Background/Objectives: Current international guidelines recommend choosing an empirical antibiotic regimen for treating diabetic foot infections (DFI) based largely on clinical severity of the infection and local microbiological epidemiology. This ma...
Removing head louse eggshells and nits after a successful pediculicide treatment is often made more difficult because it is not easy to see them all amongst the hairs. Therefore, a treatment that makes louse eggs more visible potentially makes the ta...
Background: Corneal fluorescein staining is a key biomarker in evaluating dry eye disease. However, subjective scales of corneal fluorescein staining are lacking in consistency and increase the difficulties of an accurate diagnosis for clinicians. Th...
This study focused on identifying the effect of the laser wavelengths used in cleaning some manifestation damage appearing in pigments used on archaeological cartonnage preserved in the Egyptian Museum, Egypt. The manifestations of damage appear as m...
Stem cuttings are often exploited for asexual propagation of cycad plants, and impaired health of the source plants may reduce success. A reliable procedure that screens potential source plants for predicted success would benefit conservationists. Th...
Significance: The development of an imaging technique to accurately identify biofilm regions on tissues and in wounds is crucial for the implementation of precise surface-based treatments, leading to better patient outcomes and reduced chances of inf...
In recent years, geological disasters have frequently occurred on basarlt highway slopes. Studying the stability of highway slopes in this type of area is of great significance for traffic safety. However, due to the high cost and low efficiency of t...
Histopathological image analysis is an examination of tissue under a light microscope for cancerous disease diagnosis. Computer-assisted diagnosis (CAD) systems work well by diagnosing cancer from histopathology images. However, stain variability in...
Background: Epithelial cell adhesion molecule (epcam) is a multifunctional transmembrane glycoprotein expressed on both normal epithelium and epithelial neoplasms such as gastric, breast, and renal carcinomas. Recent studies have proposed that the pr...
Accurate assessment of sperm morphology is essential for artificial insemination using liquid-preserved boar semen. This study compared nine commonly used staining techniques, eosin, eosin–nigrosin, Diff-Quick®, Hemacolor®, Sangodiff-G&...
The human skin sebum suggests that it (along with other epidermal surface lipids) plays a role in skin barrier formation, the moderation of cutaneous inflammation, and antimicrobial defense. Various methods have been developed for collecting and meas...
We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2@Cu2O-Au nanop...
Washing performance depends on the specific interactions between textiles, stains, detergents, mechanical action, temperature, and time. Its evaluation therefore requires a fundamental and practical understanding of the effects of the washing paramet...
Glycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA are employed in immunohistochemical staining during post-mortem examination: Through this method, it is possible to point out the RB...
This prospective, experimental, randomised, assessor-blinded cadaveric study was undertaken to describe the sono-anatomical features of the radial, ulnar, median and musculocutaneous (RUMM) nerves in rabbits and to develop and evaluate an ultrasound...
Live imaging of neuromuscular junctions (NMJs) in situ has been constrained by the suitability of ligands for inert vital staining of motor nerve terminals. Here, we constructed several truncated derivatives of the tetanus toxin C-fragment (TetC) fus...
High-quality three-dimensional (3D) microscopy allows detailed, unrestricted and non-destructive imaging of entire volumetric tissue specimens and can therefore increase the diagnostic accuracy of histopathological tissue analysis. However, commonly...
The existing research deals with the process of modifying polyester knitted fabrics and polyester/cotton knitted fabrics with chitosan and the stability of functionalized surface with chitosan in the washing process according to a standard and an inn...
The Myrteacae family is known as a rich source of phloroglucinols, a group of secondary metabolites with notable biological activities. Leaves of Psidium cattleianum were extracted with chloroform: methanol 8:2 to target the isolation of phloroglucin...
The aim of the study was to assess the morphometry of sperm during storage of liquid boar semen at 17 °C. An attempt was also made to evaluate the suitability of three staining methods for assessment of boar sperm morphometry. The study was carri...
Background: Folate receptor alpha (FRα) is a glycosylphosphatidylinositol-anchored membrane protein encoded by the FOLR1 gene. Its overexpression in various cancers, including ovarian carcinoma, makes it a promising target for antibody-drug con...
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