A Real-Time Detection Device for the Rapid Quantification of Skin Casual Sebum Using the Oil Red O Staining Method
Abstract
:1. Introduction
2. Materials and Methods
2.1. Skin Sebum Collection and Staining
- (1)
- Prepare the materials for collection of skin sebum
- (2)
- Prepare the Oil Red O staining solution and buffer solution
- (3)
- ORO staining
- (4)
- Absorbance of ORO-stained sebum
2.2. The RGB Color Analysis
- (1)
- Digitization of stained color data
2.3. Color Detection using Sensor
- (1)
- Hardware and RGB color sensor
- (2)
- Software and the application
3. Results and Discussion
3.1. Selection of Materials for Facial Casual Skin Sebum Collection
3.2. Comparison with Sebumeter®
3.3. Detection of Facial Casual Skin Sebum Using Color Sensor
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
- Li, X.; He, C.; Chen, Z.; Zhou, C.; Gan, Y.; Jia, Y. A review of the role of sebum in the mechanism of acne pathogenesis. J. Cosmet. Dermatol. 2017, 16, 168–173. [Google Scholar] [CrossRef] [PubMed]
- Jia, Y.; Gan, Y.; He, C.; Chen, Z.; Zhou, C. The mechanism of skin lipids influencing skin status. J. Dermatol. Science 2018, 1, 112–119. [Google Scholar] [CrossRef] [PubMed]
- Spick, M.; Longman, K.; Frampas, C.; Lewis, H.; Costa, C.; Walters, D.D.; Stewart, A.; Wilde, M.; Greener, D.; Evetts, G.; et al. Changes to the sebum lipidome upon COVID-19 infection observed via rapid sampling from the skin. EClinicalMedicine 2021, 33, 100786. [Google Scholar] [CrossRef] [PubMed]
- Shetage, S.S.; Traynor, M.J.; Brown, M.B.; Raji, M.; Graham-Kalio, D.; Chilcott, R.P. Effect of ethnicity, gender and age on the amount and composition of residual skin surface components derived from sebum, sweat and epidermal lipids. Skin Res. Technol. 2014, 20, 97–107. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- De Luca, C.; Valacchi, G. Surface lipids as multifunctional mediators of skin responses to environmental stimuli. Mediat. Inflamm. 2010, 2010, e321494. [Google Scholar] [CrossRef] [PubMed]
- Collison, D.W.; Burns, T.L.; Stewart, M.E.; Downing, D.T.; Strauss, J.S. Evaluation of a method for measuring the sustainable rate of sebaceous wax ester secretion. Arch. Dermatol. Res. 1987, 279, 266–269. [Google Scholar] [CrossRef] [PubMed]
- Wójcik, A.; Budzisz, E.; Rotsztejn, H. Skin surface lipids and their measurements. Post. Dermatol. Alergol. 2011, 6, 498–505. [Google Scholar]
- Crowther, J.M. Method for quantification of oils and sebum levels on skin using the Sebumeter®. Int. J. Cosmet. Sci. 2016, 38, 210–216. [Google Scholar] [CrossRef] [PubMed]
- Clarys, P.M.; Barel, A.O. Sebumetry: A comparison between lipid collection techniques. Skin Res. Technol. 1996, 2, 222. [Google Scholar]
- Blanc, D.; Agache, P. Sebum Excretion. Methods of measurement and influence of physical factors. Int. J. Cosmet. 1980, 25, 243–250. [Google Scholar] [CrossRef] [PubMed]
- French, R.W. Fat stains. Stain. Tech. 1926, 1, 7. [Google Scholar]
- Guigui, K.; Beaudoin, A. The use of oil red O in sequence with other methods of fingerprint development. J. For. Ident. 2007, 57, 550–581. [Google Scholar]
- Beaudoin, A. Oil red O: Fingerprint development on a 21-year old cold case. J. Forensic Ident. 2011; 61, 50–59. [Google Scholar]
- Gurvinder, S.B.; Gurvindr, S.S.; Jasjeet, K. Oil Red O (ORO) reagent for detection of latent fingermarks: A review. Egypt J. Forensic Sci. 2019, 9, 3. [Google Scholar]
- Frick, A.; Fritz, P.; Lewis, S.; Van Bronswijk, W. Sequencing of a modified oil red O development technique for the detection of latent fingermarks on paper surfaces. J. Forensic Ident. 2013, 63, 369–385. [Google Scholar]
- Frick, A.A.; Fritz, P.; Lewis, S.W.; van Bronswijk, W. A modified Oil Red O (ORO) formulation for the detection of latent fingermarks on porous substrates. J. Forensic Ident. 2012, 62, 623–641. [Google Scholar]
- Honig, M.; Yoak, J. Oil red O: A comparative performance study. J. Forensic Ident. 2016, 66, 118–133. [Google Scholar]
- Beaudoin, A. Fingerprint staining technique on dark and wetted porous surfaces: Oil red O and rhodamine 6G. J. Forensic Ident. 2012, 62, 315–329. [Google Scholar]
- Cressey, D. Age of the Arduino. Nature 2017, 544, 125–126. [Google Scholar] [CrossRef] [PubMed]
- Rode, B.; Ivens, U.; Serup, J. Degreasing method for the seborrheic areas with respect to regaining sebum excretion rate to casual level. Skin Res. Technol. 2000, 6, 92–99. [Google Scholar] [CrossRef] [PubMed]
- Cunliffe, W.J.; Shuster, S. The rate of sebum excretion in man. Br. J. Dermatol. 1969, 81, 697–704. [Google Scholar] [CrossRef] [PubMed]
Sample | Sebum Replace Time | Color Value Using Image J | R² |
---|---|---|---|
PM1 | After washing 0 min | 11.6995 | 0.9654 |
30 min | 19.791 | ||
60 min | 35.778 | ||
PM2 | After washing 0 min | 22.6905 | 0.9523 |
30 min | 26.699 | ||
60 min | 35.7805 | ||
PF1 | After washing 0 min | 29.9695 | 0.4083 |
30 min | 29.2205 | ||
60 min | 31.35 | ||
PF2 | After washing 0 min | 26.005 | 0.9789 |
30 min | 39.1375 | ||
60 min | 46.9415 |
Sample | Sebum Replace Time | Color Value Using Image J | Sebumeter | Correlation |
---|---|---|---|---|
PM3 | After washing 0 min | 8.7 | 8 | 0.946 |
30 min | 47.2945 | 40 | ||
60 min | 53.224 | 65 | ||
PM4 | After washing 0 min | 10.8 | 68 | 0.996 |
30 min | 62.6 | 87 | ||
60 min | 56.1 | 83 | ||
PF3 | After washing 0 min | 10.8 | 31 | 0.994 |
30 min | 30.48 | 74 | ||
60 min | 68.5 | 131 |
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Ahn, K.; Han, S.; Yun, K.; Lee, W.; Lee, D.-G.; Kang, S.M.; Choi, Y.-B.; Han, K.; Ahn, Y.J. A Real-Time Detection Device for the Rapid Quantification of Skin Casual Sebum Using the Oil Red O Staining Method. Sensors 2022, 22, 3016. https://doi.org/10.3390/s22083016
Ahn K, Han S, Yun K, Lee W, Lee D-G, Kang SM, Choi Y-B, Han K, Ahn YJ. A Real-Time Detection Device for the Rapid Quantification of Skin Casual Sebum Using the Oil Red O Staining Method. Sensors. 2022; 22(8):3016. https://doi.org/10.3390/s22083016
Chicago/Turabian StyleAhn, Kung, Sangjin Han, Kyeongeui Yun, Wooseok Lee, Dong-Geol Lee, So Min Kang, Young-Bong Choi, Kyudong Han, and Yong Ju Ahn. 2022. "A Real-Time Detection Device for the Rapid Quantification of Skin Casual Sebum Using the Oil Red O Staining Method" Sensors 22, no. 8: 3016. https://doi.org/10.3390/s22083016
APA StyleAhn, K., Han, S., Yun, K., Lee, W., Lee, D.-G., Kang, S. M., Choi, Y.-B., Han, K., & Ahn, Y. J. (2022). A Real-Time Detection Device for the Rapid Quantification of Skin Casual Sebum Using the Oil Red O Staining Method. Sensors, 22(8), 3016. https://doi.org/10.3390/s22083016