Special Issue "Current Status and Advances in Semen Preservation"
A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".
Deadline for manuscript submissions: 31 May 2022.
Special Issue Editors
Interests: animal reproduction; sperm quality; liquid storage; cryopreservation; membrane integrity; antioxidants; energy metabolism; proteomic
Interests: animal reproduction; andrology; sperm quality assessment; flow cytometry; CASA system; semen cryopreservation; antioxidants
Special Issue Information
Dear Colleagues,
The scope of this Special Issue concerns one of the assisted reproductive techniques (ARTs), which have been developing very dynamically in recent years and are becoming more and more effective. ARTs include, among others, the storage of gametes and embryos, in vitro fertilization (IVF), artificial insemination (AI) and embryo transfer (ET). The storage of gametes, especially sperm, is a commonly used method in the reproduction of farm, companion and working animals, as well as wild-living ones. The choice of the preferred sperm preservation method (liquid or frozen state) is species-determined. Therefore, the improvement of sperm preservation methods requires taking into account species differences and the influence of various factors, e.g., the temperature and time of storage or type of diluent used. Sperm cryopreservation (ejaculated and epididymal) allows for the long-term preservation of valuable genetic material for reproductive purposes. The evaluation of the quality of preserved sperm requires the use of a wide spectrum of laboratory analyses (including computer-assisted, fluorescent or proteomic analyses). The use of an appropriate preservation procedure allows sperm to maintain fertilizing ability, which is predicted by the results of laboratory analyses and confirmed by studies with their use in vitro and in AI procedures.
We particularly welcome manuscripts that present new technological solutions for the improvement of sperm preservation methods and show new possibilities for using various laboratory analyses to predict the fertilizing potential of preserved sperm. We hope this issue will provide new insights into the progress made in the preservation of various animal species’ semen.
Dr. Anna Dziekońska
Dr. Agnieszka Partyka
Guest Editors
Manuscript Submission Information
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Keywords
- semen preservation
- liquid state
- cryopreservation
- semen quality
- fertility
- assisted reproductive technologies
- energy metabolism
- antioxidants
- proteomic
Planned Papers
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Article Type Review
Title Antimicrobial pe ptides in boar semen extender the alternative use for standard antibiotics
Abstract Artificial insemination (AI) is standard method in a ommercial swine production. For insemination there is used liquid preserved semen which can be stored, but it depends on the extenders at 15 17°C at least several days. Due to the rich composition of the extender and storage temperature, the extender is a good enviroment for the growth of microorganisms. At present, the addition of antibiotics in semen extenders is the only way to prevent bacteriospermia. Commonly used antibiotics increase the antibio tic resistance, and additionally have a negative effect on sperm quality. Therefore, it is justified to search new biopreparations with an antimicrobial activity. It is imperative that such an antibiotic substitute achieves the maximum antibacterial effect and does not have a negative effect on sperm quality High expectations are associated with AMP (antimicrobial peptides) showing an antibacterial, antiviral, antifungal and an antiparasitic activity. The big benefit of using AMP as opposed to antibiotics is the absence of microbial resistance to these peptides. The article presents current reaserch in using AMP as an alternative to standard antiobiotics in a boar semen extender.
Authors Monika Trzcińska, Magdalena Bryła
Affiliation Department of Reproductive Bio technology and Cryoconservation, National
Research Institute of Animal Production, Krakowska Str. 1, 32 083 Balice, Poland
II. Article Type: Original
Title: : High hydrostatic pressure: the way to improve High hydrostatic pressure: the way to improve the quality of cryopreserved poor boar the quality of cryopreserved poor boar ejaculatesejaculates
Authors Monika Trzcińska, Magdalena Bryła
Affiliation Department of Reproductive Bio technology and Cryoconservation, National
Research Institute of Animal Production, Krakowska Str. 1, 32 083 Balice, Poland
3.ORIGINAL PAPER
Spermatozoa survival at room and chilling temperature in domestic turkeys as model of endangered peacocks.
Alkali I.M.1,2, Asuku S.O.1, Colombo M.2, Bukar M.M.1, Waziri M.A.1 and Luvoni G.C.2
1 Department of Theriogenology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria
2 Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Università degli Studi di Milano, Italy
Populations of many galliform species have declined mainly due to habitat loss and over-hunting notably, the Congo peacock (Afropavo congensis) which has been classified as a vulnerable species by the International Union for Conservation of Nature (IUCN). The domestic turkey (Meleagris gallopavo) being a species of least concern could serve as a model for optimizing assisted reproductive technologies for the Congo peacock. This study is aimed at developing turkey semen extender suitable for assisted reproductive technologies in field conditions. Semen was collected using the dorso-abdominal massage technique from seven turkey toms (8-15 kg) and analysed. Ejaculates with > 70% motility and ≥ 80% live spermatozoa were pooled and divided into 5 aliquots (4 treatments and 1 control). Each of the four treatments was extended in soybean based extender (SBR), egg yolk based extender (EYR), and L ascorbic acid supplemented SBR and EYR respectively. Two liquid preservation protocols [room temperature (25 °C) and chilled (4 °C)] were employed and quality parameters including motility, viability and morphology were evaluated. We conclude that, the two extenders (egg yolk based and soybean based) were similar with regard to in vitro semen quality parameters and could be used to preserve turkey semen and L ascorbic acid supplementation of the turkey semen extenders improved semen quality during in vitro liquid storage.
Cryopreservation of giraffe sperm
Hermes, Robert