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20,006 Results Found

  • Article
  • Open Access
3 Citations
3,097 Views
13 Pages

20 June 2022

Zooplankton community data from 45 dimictic lakes, representing homogenous abiotic conditions, were used to distinguish indicator taxa of near-pristine, reference lakes with low anthropopression. Reference conditions were selected based on natural la...

(This article belongs to the Special Issue Diversity and Ecology of Zooplankton in Lake Subhabitats)
  • Article
  • Open Access
2 Citations
724 Views
17 Pages

Screening and Identification of Reference Genes Under Different Conditions and Growth Stages of Lyophyllum decastes

  • Yun-Qi Hui,
  • Huan-Ling Yang,
  • Yu-Qing Zhang,
  • Chen-Zhao Zhu and ,
  • Li-Ping Xi,
  • Chun-Yan Song,
  • Zheng-Peng Li,
  • E-Xian Li,
  • Shu-Hong Li and
  • Rui-Heng Yang
  • + 6 authors+ 1 author

13 November 2025

Internal reference genes are a prerequisite for ensuring the accuracy of gene verification experiments, but few relevant studies on Lyophyllum decastes have investigated the growth cycle and different environmental conditions. In this study, the qPCR...

(This article belongs to the Section Molecular Plant Sciences)
  • Article
  • Open Access
6 Citations
4,079 Views
11 Pages

The nutrient reference conditions of lakes play a key role for lake water quality control and water resource management. The inferential models are important methods for calculating reference values; however, the dependence and “cluster” in time seri...

(This article belongs to the Special Issue Water Resources Systems Quality and Quantity Management)
  • Feature Paper
  • Article
  • Open Access
6 Citations
3,832 Views
19 Pages

20 September 2021

Under the EU Water Framework Directive, ecological assessment and management are based on type-specific reference conditions. In the EU it may be difficult to find sites in large rivers with at least near-natural conditions, though this is not the ca...

(This article belongs to the Section Biodiversity and Functionality of Aquatic Ecosystems)
  • Article
  • Open Access
2 Citations
1,384 Views
15 Pages

A Unified Generalized Extreme Value Distribution Framework for Estimating Lake Reference Nutrient Conditions with Confidence Intervals: A Case Study of Hongze Lake, China

  • Anan Wang,
  • Haomiao Cheng,
  • Wei Jiang,
  • Wei Ma and ,
  • Fukang Yang,
  • Lihua Zhang,
  • Xiaohong Jiang and
  • Liang Wang
  • + 3 authors+ 0 author

14 May 2025

The scientific determination of reference conditions for lake nutrients is fundamental for establishing ecologically sustainable nutrient criteria. This study developed a novel method for determining lake reference nutrient conditions based on genera...

(This article belongs to the Section Sustainable Water Management)
  • Article
  • Open Access
41 Citations
4,592 Views
23 Pages

Selection of Suitable Reference Genes for RT-qPCR Gene Expression Analysis in Siberian Wild Rye (Elymus sibiricus) under Different Experimental Conditions

  • Junchao Zhang,
  • Wengang Xie,
  • Xinxuan Yu,
  • Zongyu Zhang and ,
  • Yongqiang Zhao,
  • Na Wang and
  • Yanrong Wang
  • + 2 authors+ 0 author

13 June 2019

Elymus sibiricus, which is a perennial and self-pollinated grass, is the typical species of the genus Elymus, which plays an important role in forage production and ecological restoration. No reports have, so far, systematically described the selecti...

(This article belongs to the Section Plant Genetics and Genomics)
  • Article
  • Open Access
10 Citations
6,488 Views
17 Pages

Assessing the Robustness of Pan Evaporation Models for Estimating Reference Crop Evapotranspiration during Recalibration at Local Conditions

  • Konstantinos Babakos,
  • Dimitris Papamichail,
  • Panagiotis Tziachris,
  • Vassilios Pisinaras and ,
  • Kleoniki Demertzi and
  • Vassilis Aschonitis
  • + 1 author+ 0 author

1 September 2020

A classic method for assessing the reference crop evapotranspiration (ETo) is the pan evaporation (Epan) method that uses Epan measurements and pan coefficient (kp) models, which can be functions of relative humidity (RH), wind speed (u2), and temper...

(This article belongs to the Section Hydrological Measurements and Instrumentation)
  • Article
  • Open Access
1,755 Views
15 Pages

Quantitative Evaluation of Endogenous Reference Genes for RT-qPCR and ddPCR Gene Expression Under Polyextreme Conditions Using Anaerobic Halophilic Alkalithermophile Natranaerobius thermophilus

  • Xinyi Tao,
  • Qinghua Xing,
  • Yingjie Zhang,
  • Belsti Atnkut and ,
  • Haozhuo Wei,
  • Silva Ramirez,
  • Xinwei Mao and
  • Baisuo Zhao
  • + 3 authors+ 0 author

Accurate gene expression quantification using reverse transcription quantitative PCR (RT-qPCR) requires stable reference genes (RGs) for reliable normalization. However, few studies have systematically identified RGs suitable for simultaneous high sa...

(This article belongs to the Section Environmental Microbiology)
  • Article
  • Open Access
23 Views
14 Pages

Reference measurements can affect battery state-of-health (SOH) estimates across operating conditions. We compare reference protocols using fixed local discharge features, regression settings, training records, and evaluation rules. XJTU Batch-3 prov...

(This article belongs to the Section Storage Systems)
  • Article
  • Open Access
10 Citations
3,738 Views
12 Pages

Determining Soil Nutrients Reference Condition in Alpine Region Grassland, China: A Case Study of Hulun Buir Grassland

  • Jiaxun Li,
  • Feifei Cao,
  • Di Wu,
  • Xiao Fu and ,
  • Ye Tian and
  • Gang Wu
  • + 1 author+ 0 author

7 December 2018

Grasslands represent a source of nutrient cycling, ecosystem stabilization, and services for human use. Grassland soil quality is commonly used as an indicator of ecosystem health and sustainability due to its strong correlation with overall grasslan...

  • Article
  • Open Access
142 Views
19 Pages

30 October 2026

Cross-country EV-battery indicators require scrutiny before they can support physical or policy conclusions. We examine the proposed demand-condition gap (DCG) through a methodological stress test and measurement admissibility assessment for 30 count...

(This article belongs to the Special Issue Towards a Sustainable Electric Vehicle Ecosystem: Challenges and Innovations)
  • Article
  • Open Access
19 Citations
1,635 Views
20 Pages

Sustainable Hydrochemical Reference Conditions in the Headwaters of Western Ukraine

  • Olha Biedunkova,
  • Pavlo Kuznietsov,
  • Oksana Tsos,
  • Mariia Boiaryn and
  • Olha Karaim

14 January 2026

Establishing reliable hydrochemical reference conditions is essential for water quality assessment and for the implementation of the European Union Water Framework Directive, particularly in regions where biological and hydromorphological data remain...

(This article belongs to the Section Environmental Sustainability and Applications)
  • Review
  • Open Access
22 Citations
4,262 Views
12 Pages

The EuRRECa Project as a Model for Data Access and Governance Policies for Rare Disease Registries That Collect Clinical Outcomes

  • Salma R. Ali,
  • Jillian Bryce,
  • Li En Tan,
  • Olaf Hiort and ,
  • Alberto M. Pereira,
  • Erica L. T. van den Akker,
  • Natasha M. Appelman-Dijkstra,
  • Jerome Bertherat,
  • Martine Cools and
  • S. Faisal Ahmed
  • + 11 authors+ 6 authors

Rare disease (RD) registries are important platforms that facilitate communication between health care professionals, patients and other members of the multidisciplinary team. RD registries enable data sharing and promotion of research and audits, of...

(This article belongs to the Special Issue Rare Diseases Epidemiology Research: The State-of-the-Art and Future Perspectives)
  • Article
  • Open Access
5 Citations
3,459 Views
15 Pages

Evaluation of Reference Genes for Quantitative PCR in Eustoma grandiflorum under Different Experimental Conditions

  • Wanjie Xue,
  • Lishan Wang,
  • Xueqi Li,
  • Mingwei Tang and ,
  • Jingyao Li,
  • Bing Ding,
  • Saneyuki Kawabata,
  • Yuhua Li and
  • Yang Zhang
  • + 4 authors+ 0 author

Eustoma grandiflorum, commonly known as prairie gentian or Texas bluebells, is among the most popular agriculturally propagated species of cut flowers. Due to its widespread appeal, there is increasing interest in understanding the molecular genetic...

  • Article
  • Open Access
21 Citations
7,962 Views
15 Pages

Identification of Reference and Biomarker Proteins in Chlamydomonas reinhardtii Cultured under Different Stress Conditions

  • Jianan Shi,
  • Teng Huang,
  • Shuaijie Chai,
  • Yalu Guo and ,
  • Jian Wei,
  • Shijuan Dou,
  • Liyun Li and
  • Guozhen Liu
  • + 3 authors+ 0 author

Reference proteins and biomarkers are important for the quantitative evaluation of protein abundance. Chlamydomonas reinhardtii was grown under five stress conditions (dark, cold, heat, salt, and glucose supplementation), and the OD750 and total prot...

(This article belongs to the Special Issue Selected Papers from the 6th National Plant Protein Research Congress)
  • Article
  • Open Access
21 Citations
4,235 Views
14 Pages

Selection and Validation of Reference Genes for qRT-PCR in Lentinula edodes under Different Experimental Conditions

  • Yi Luo,
  • Gangzheng Wang,
  • Chen Wang,
  • Yuhua Gong and ,
  • Yinbing Bian and
  • Yan Zhou
  • + 1 author+ 0 author

27 August 2019

Lentinula edodes is the most consumed mushroom in Asia due to its nutritional and medicinal values, and the optimal reference gene is crucial for normalization of its gene expression analysis. Here, the expression stability of 18 candidate reference...

(This article belongs to the Section Plant Genetics and Genomics)
  • Article
  • Open Access
1 Citations
463 Views
19 Pages

Screening of Reference Gene for RT-qPCR in Leymus chinensis During Environmental Stress Conditions

  • Jinfang Li,
  • Dongli Wan,
  • Jinhua Liu,
  • Chaoqun Zhang and
  • Yongqing Wan

Reliable reference genes are critical for ensuring the accuracy of RT-qPCR-based gene expression analysis, especially under environmental stress conditions. In this study, Leymus chinensis was used as the experimental material, and eight candidate re...

(This article belongs to the Section Molecular Biology)
  • Article
  • Open Access
3 Citations
3,666 Views
18 Pages

Long-Term Assessment of Reference Baselines for the Determination of the Crop Water Stress Index in Maize under Mediterranean Conditions

  • Alejandro Prior,
  • Orly Enrique Apolo-Apolo,
  • Pedro Castro-Valdecantos,
  • Manuel Pérez-Ruiz and
  • Gregorio Egea

5 November 2021

Canopy temperature has been proposed as a relevant variable for crop water stress monitoring. Since crop temperature is highly influenced by the prevailing climatic conditions, it is usually normalized with indices such as the crop water stress index...

(This article belongs to the Section Water, Agriculture and Aquaculture)
  • Article
  • Open Access
18 Citations
3,172 Views
19 Pages

19 November 2020

Ginkgo biloba, a deciduous tree species in the Ginkgo family, has a long history of cultivation in China and is widely used in garden landscapes, medicine, food, and health products. However, few reports have focused on the systematic selection of op...

(This article belongs to the Section Forest Ecophysiology and Biology)
  • Article
  • Open Access
181 Views
68 Pages

22 October 2026

VibroAgent represented ten-second acceleration windows from one reference and five monitored locations as 84 continuous soft tokens for Gemma 4 E2B, letting one model decide and potentially explain; only its decisions were evaluated. Tasks were globa...

(This article belongs to the Special Issue AI in Complex Engineering Systems)
  • Article
  • Open Access
14 Citations
3,784 Views
15 Pages

Screening Potential Reference Genes in Tuta absoluta with Real-Time Quantitative PCR Analysis under Different Experimental Conditions

  • An-Pei Yang,
  • Yu-Sheng Wang,
  • Cong Huang,
  • Zhi-Chuang Lv and ,
  • Wan-Xue Liu,
  • Si-Yan Bi,
  • Fang-Hao Wan,
  • Qiang Wu and
  • Gui-Fen Zhang
  • + 4 authors+ 0 author

17 August 2021

Tuta absoluta is one of the most significant invasive pests affecting tomato plants worldwide. RT-qPCR has emerged as one of the most sensitive and accurate methods for detecting gene expression data. The screening of stable internal reference genes...

(This article belongs to the Section Animal Genetics and Genomics)
  • Article
  • Open Access
6 Citations
3,248 Views
15 Pages

Selection and Validation of the Most Suitable Reference Genes for Quantitative Real-Time PCR Normalization in Salvia rosmarinus under In Vitro Conditions

  • Rohit Bharati,
  • Madhab Kumar Sen,
  • Ram Kumar,
  • Aayushi Gupta and ,
  • Vishma Pratap Sur,
  • Ingrid Melnikovová and
  • Eloy Fernández-Cusimamani
  • + 2 authors+ 0 author

27 October 2022

Salvia rosmarinus L. (rosemary) is known to have a wide range of pharmacological effects including antidiabetic, anticarcinogenic, and antitumorigenic properties owing to its secondary metabolites. Studies aiming to elevate these metabolites have uti...

(This article belongs to the Section Plant Molecular Biology)
  • Article
  • Open Access
269 Views
23 Pages

27 September 2026

A defensible local global navigation satellite system (GNSS) reference requires more than a precise network adjustment when small position or displacement changes are interpreted against that reference. This study evaluates a quality-audited realisat...

(This article belongs to the Special Issue Satellite Geodesy and Earth System Monitoring)
  • Article
  • Open Access
4 Citations
5,168 Views
22 Pages

Immunotherapy has emerged as a key modality in cancer treatment, yet its effectiveness varies significantly among patients, often due to the metabolic stress imposed by the tumor microenvironment. Hypoxia, a major factor in the tumor microenvironment...

(This article belongs to the Section Molecular Immunology)
  • Article
  • Open Access
19 Citations
3,864 Views
14 Pages

15 May 2022

Plagiodera versicolora (Coleoptera: Chrysomelidae) is one of the most destructive pests of the Salicaceae worldwide, which has established complex interactions with surrounding organisms. Uncovering the molecular mechanisms of some antagonistic inter...

(This article belongs to the Special Issue Biological Interactions of Pests)
  • Article
  • Open Access
11 Citations
3,763 Views
15 Pages

Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (Fragaria × ananassa) Leaves under Different Stress and Light-Quality Conditions

  • Yuntian Ye,
  • Yang Lu,
  • Guangyi Wang,
  • Yongqiang Liu and ,
  • Yunting Zhang and
  • Haoru Tang
  • + 1 author+ 0 author

Selecting an appropriate reference gene is of crucial importance for improving the accuracy of qRT-PCR analyses. In this study, strawberry (Fragaria ananassa) seedlings were subjected to different environmental conditions including heat, cold, drough...

  • Article
  • Open Access
5 Citations
3,355 Views
13 Pages

Selection and Validation of Reliable Reference Genes for qRT-PCR Normalization of Bursaphelenchus xylophilus from Different Temperature Conditions and Developmental Stages

  • Yajing Wu,
  • Jiao Zhou,
  • Yaning Liu,
  • Yutong Gu and ,
  • Hongxia Zhang,
  • Faheem Ahmad,
  • Guochang Wang and
  • Lili Ren
  • + 3 authors+ 0 author

11 March 2022

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a powerful technique for studying gene expression. The key to quantitative accuracy depends on the stability of the reference genes used for data normalization under different...

(This article belongs to the Section Agricultural Science and Technology)
  • Article
  • Open Access
3 Citations
2,979 Views
14 Pages

Stability and Chemical Conversion of the Purified Reference Material of Gymnodimine-A under Different Temperature and pH Conditions

  • Guixiang Wang,
  • Jiangbing Qiu,
  • Aifeng Li,
  • Ying Ji and ,
  • Zhixuan Tang and
  • Philipp Hess
  • + 1 author+ 0 author

29 October 2022

Gymnodimines (GYMs) are a group of fast-acting phycotoxins and their toxicological effects on human beings are still unclear due to the lack of sufficiently well-characterized large quantities of purified toxins for toxicology studies. In this study,...

(This article belongs to the Section Marine and Freshwater Toxins)
  • Article
  • Open Access
1,073 Views
19 Pages

Screening and Stability Validation of RT-qPCR Reference Genes in Portulaca oleracea L. in Diverse Tissues and Under Abiotic Stress Conditions

  • Jiahui Fang,
  • Chenxin Fan,
  • Jieshan Wang,
  • Ming Yi and ,
  • Ping Li,
  • Mengyun Xu and
  • Jian Yan
  • + 2 authors+ 0 author

28 February 2026

Purslane (Portulaca oleracea L.) is an important plant species that has been increasingly used in functional gene studies and molecular analyses. However, reference genes that exhibit stable expression across multiple tissues and stress conditions ha...

(This article belongs to the Section Molecular Biology)
  • Article
  • Open Access
26 Citations
7,423 Views
16 Pages

Changing Land Use and Population Density Are Degrading Water Quality in the Lower Mekong Basin

  • Flavia Tromboni,
  • Thomas E. Dilts,
  • Sarah E. Null,
  • Sapana Lohani and ,
  • Peng Bun Ngor,
  • Savoeurn Soum,
  • Zeb Hogan and
  • Sudeep Chandra
  • + 3 authors+ 0 author

15 July 2021

Establishing reference conditions in rivers is important to understand environmental change and protect ecosystem integrity. Ranked third globally for fish biodiversity, the Mekong River has the world’s largest inland fishery providing livelihoods, f...

(This article belongs to the Special Issue Channels for Change: Integrating Multiple Disciplines for New Frontiers in Managing the Mekong River Basin)
  • Article
  • Open Access
13 Citations
5,119 Views
22 Pages

31 May 2023

Lindera megaphylla, a broad-leaved evergreen that is used as a landscape ornamental plant and medicinal plant, is an ecologically important and dominant tree species. However, little is known about the molecular mechanisms of its growth, development,...

(This article belongs to the Special Issue Molecular Biology of Ornamental Plants)
  • Article
  • Open Access
3 Citations
2,709 Views
22 Pages

Selection and Validation of Reference Genes for RT-qPCR in Protonemal Tissue of the Desiccation-Tolerant Moss Pseudocrossidium replicatum Under Multiple Abiotic Stress Conditions

  • Rosa María Nava-Nolazco,
  • Selma Ríos-Melendez,
  • Santiago Valentín Galván-Gordillo,
  • Angélica C. Martínez-Navarro and ,
  • Mishael Sánchez-Pérez,
  • Rocio Alejandra Chavez-Santoscoy,
  • Martha Bibbins-Martínez,
  • Ignacio Eduardo Maldonado-Mendoza,
  • Analilia Arroyo-Becerra and
  • Miguel Angel Villalobos-López
  • + 5 authors+ 0 author

7 June 2025

Plant abiotic stresses are the main cause of significant crop losses worldwide. The moss Pseudocrossidium replicatum is highly tolerant to different types of abiotic stress, such as desiccation. Our group is interested in identifying and characterisi...

(This article belongs to the Special Issue Plant Stress Physiology and Molecular Biology—2nd Edition)
  • Opinion
  • Open Access
7 Citations
4,695 Views
6 Pages

The intense human pressures in the Anthropocene epoch are causing an alarming decline in marine coastal ecosystems and an unprecedented loss of biodiversity. This situation underscores the urgency of making ecological restoration a global priority to...

  • Article
  • Open Access
4 Citations
7,477 Views
20 Pages

2 February 2023

The Craig–Bampton method is frequently applied in most commercial multibody dynamic software. Nevertheless, the Craig–Bampton modes only represent the free-free modes in flexible multibody systems. However, the free-free modes are incapab...

(This article belongs to the Special Issue Modeling, Dynamics, Intelligent Control, and Fault Diagnosis of Vehicle Systems)
  • Article
  • Open Access
7 Citations
3,683 Views
27 Pages

Reference Values of the Quality of Life after Brain Injury (QOLIBRI) from a General Population Sample in Italy

  • Ugne Krenz,
  • Sven Greving,
  • Marina Zeldovich,
  • Juanita Haagsma and ,
  • Suzanne Polinder,
  • Nicole von Steinbüchel and
  • on behalf of the CENTER-TBI Participants and Investigators
  • + 2 authors+ 0 author

6 January 2023

Traumatic brain injury (TBI) may affect the lives of the individuals concerned and their relatives negatively in many dimensions. Health-related quality of life (HRQoL) is a comprehensive and complex concept that can assess one’s satisfaction w...

(This article belongs to the Special Issue Traumatic Brain Injury (TBI): Recent Trends and Future Perspectives)
  • Article
  • Open Access
539 Views
19 Pages

Identification and Validation of Reference Genes for Reliable RT-qPCR Normalization in Schisandra chinensis Across Different Tissues and Abiotic Stress Conditions

  • Longjun Liang,
  • Xin Song,
  • Xuanhe Zhang,
  • Yingchun Liu and ,
  • Guangli Shi,
  • Zhenxing Wang,
  • Cong Zhang,
  • Chengzhan Li,
  • Xiyu Zhang and
  • Jun Ai
  • + 6 authors+ 1 author

24 June 2026

Reverse transcription quantitative real-time PCR (RT-qPCR) is a highly efficient and sensitive technique for quantifying gene transcript levels. The accuracy of gene expression analysis depends critically on the selection of appropriate reference gen...

(This article belongs to the Section Plant Molecular Biology)
  • Article
  • Open Access
4 Citations
1,983 Views
13 Pages

Comprehensive Screening and Validation of Stable Internal Reference Genes for Accurate qRT-PCR Analysis in Holotrichia parallela under Diverse Biological Conditions and Environmental Stresses

  • Zhongjun Gong,
  • Jing Zhang,
  • Qi Chen,
  • Huiling Li and ,
  • Ziqi Zhang,
  • Yun Duan,
  • Yueli Jiang,
  • Tong Li,
  • Jin Miao and
  • Yuqing Wu
  • + 5 authors+ 0 author

30 August 2024

Holotrichia parallela is among the world’s most destructive pests. For accurate qPCR and gene expression studies, the selection of stable and appropriate reference genes is crucial. However, a thorough evaluation of potential reference genes fo...

(This article belongs to the Section Insect Molecular Biology and Genomics)
  • Article
  • Open Access
7 Citations
2,607 Views
20 Pages

Comprehensive Assessment of Reference Gene Expression within the Whitefly Dialeurodes citri Using RT-qPCR

  • Weizhen Kong,
  • Xiaolu Lv,
  • Xiaotong Ran,
  • Marguerite Mukangango and ,
  • Bugenimana Eric Derrick,
  • Baoli Qiu and
  • Changfei Guo
  • + 2 authors+ 0 author

28 February 2024

The citrus whitefly, Dialeurodes citri, is a destructive pest that infests citrus plants. It is a major vector in transmitting plant viruses such as citrus yellow vein clearing virus (CYVCV), which has caused severe economic losses worldwide, and the...

(This article belongs to the Section Animal Genetics and Genomics)
  • Article
  • Open Access
11 Citations
4,626 Views
16 Pages

LRP10, PGK1 and RPLP0: Best Reference Genes in Periprostatic Adipose Tissue under Obesity and Prostate Cancer Conditions

  • Jesús M. Pérez-Gómez,
  • Francisco Porcel-Pastrana,
  • Marina De La Luz-Borrero,
  • Antonio J. Montero-Hidalgo and ,
  • Enrique Gómez-Gómez,
  • Aura D. Herrera-Martínez,
  • Rocío Guzmán-Ruiz,
  • María M. Malagón,
  • Manuel D. Gahete and
  • Raúl M. Luque
  • + 5 authors+ 0 author

13 October 2023

Obesity (OB) is a metabolic disorder characterized by adipose tissue dysfunction that has emerged as a health problem of epidemic proportions in recent decades. OB is associated with multiple comorbidities, including some types of cancers. Specifical...

(This article belongs to the Special Issue Adipose Tissue and Gene Expression)
  • Article
  • Open Access
3 Citations
3,975 Views
20 Pages

Using a Reference Color Plate to Correct Smartphone-Derived Soil Color Measurements with Different Smartphones Under Different Lighting Conditions

  • Sheng Li,
  • Fangzhou Zheng,
  • Alexander J. Koiter,
  • Yulia Kupriyanovich and ,
  • David A. Lobb and
  • Masoud Goharrokhi
  • + 1 author+ 0 author

Soil color has long been used as an indicator for soil properties such as soil organic carbon and soil moisture. Recent developments in citizen science have seen the increased use of smartphone cameras for soil color measurements. However, there are...

  • Article
  • Open Access
213 Views
20 Pages

Systematic Screening of Reference Genes for qPCR in Procambarus clarkii: RPL13a Shows Stable Expression Under Non-Ionic Ammonia Stress and Multiple Experimental Conditions

  • Xiudan Yuan,
  • Xing Tian,
  • Yuanyuan Liu,
  • Zhigang He and ,
  • Jinlong Li,
  • Yunpeng Fan,
  • Jiarong Guo,
  • Yong He,
  • Shiming Deng and
  • Dongwu Wang
  • + 5 authors+ 0 author

13 October 2026

Real-time quantitative PCR (qPCR) is a key technique for elucidating the mechanisms underlying environmental stress responses in aquatic animals, and its accuracy relies heavily on stable reference genes for data normalization. In this study, histopa...

(This article belongs to the Section Marine and Freshwater Biology)
  • Article
  • Open Access
24 Citations
6,435 Views
19 Pages

Changes of Reference Evapotranspiration and Its Relationship to Dry/Wet Conditions Based on the Aridity Index in the Songnen Grassland, Northeast China

  • Qiyun Ma,
  • Jiquan Zhang,
  • Caiyun Sun,
  • Enliang Guo and ,
  • Feng Zhang and
  • Mengmeng Wang
  • + 1 author+ 0 author

29 April 2017

Reference evapotranspiration (ET0) plays an important role in regional dry/wet conditions. Based on the Food and Agriculture Organization of the United (FAO) Penman-Monteith method and daily climate variables, ET0 was calculated for 21 stations in an...

(This article belongs to the Special Issue Adaptation Strategies to Climate Change Impacts on Water Resources)
  • Feature Paper
  • Article
  • Open Access
11 Citations
4,260 Views
20 Pages

4 December 2020

The key role of paralic environments as providers of ecosystem services, associated with their increasingly threatened state, led to the definition of international water management policies aimed to improve ecological quality status (EcoQs). Restora...

(This article belongs to the Special Issue Climate Change and Anthropogenic Impact on Coastal Environments)
  • Article
  • Open Access
312 Views
27 Pages

17 September 2026

Compressed-air leaks create persistent parasitic demand, but machine-level condition monitoring is difficult because air consumption changes strongly with operating mode. LEADI (Leak Energy Anomaly Detection Index) was developed as an operating-mode-...

(This article belongs to the Special Issue Condition Monitoring and Fault Diagnosis)
  • Article
  • Open Access
4 Citations
1,114 Views
18 Pages

Model Reference Adaptive Sensorless Control of Variable-Speed Pumped Storage Doubly Fed Induction Machine Under Reversible Operations

  • Zhi Zheng,
  • Ziqiang Man,
  • Shuxin Tan,
  • Wei Yan and ,
  • Yu Lu,
  • Jie Tian,
  • Weiqun Liu and
  • Xu Wang
  • + 3 authors+ 0 author

5 June 2025

The sensorless control of doubly fed induction machine (DFIM) rotor magnetic flux based on a model reference adaptive system (MRAS) is proposed to improve the reliability of a large-scale variable-speed pumped storage (VSPS) system and reduce operati...

(This article belongs to the Special Issue Advanced Control Strategies for Multiphase Induction Generators: Design, Optimization, and Application 2024)
  • Article
  • Open Access
20 Citations
4,189 Views
29 Pages

Reference Values of the QOLIBRI from General Population Samples in the United Kingdom and The Netherlands

  • Anastasia Gorbunova,
  • Marina Zeldovich,
  • Daphne C. Voormolen,
  • Ugne Krenz and ,
  • Suzanne Polinder,
  • Juanita A. Haagsma,
  • York Hagmayer,
  • Amra Covic,
  • Ruben G. L. Real and
  • on behalf of the CENTER-TBI Participants and Investigators
  • + 7 authors+ 2 authors

The Quality of Life after Traumatic Brain Injury (QOLIBRI) instrument is an internationally validated patient-reported outcome measure for assessing disease-specific health-related quality of life (HRQoL) in individuals after traumatic brain injury (...

(This article belongs to the Section Epidemiology & Public Health)
  • Article
  • Open Access
17 Citations
3,884 Views
17 Pages

15 January 2022

In the present work, we analysed time series data on the introduction of new non-indigenous species (NIS) in the Mediterranean between 1970 and 2017, aiming to arrive at recommendations concerning the reference period and provisional threshold values...

(This article belongs to the Special Issue Biodiversity Conservation in Mediterranean Sea)
  • Article
  • Open Access
2 Citations
3,466 Views
24 Pages

Neural Architecture Comparison for Bibliographic Reference Segmentation: An Empirical Study

  • Rodrigo Cuéllar Hidalgo,
  • Raúl Pinto Elías,
  • Juan-Manuel Torres-Moreno,
  • Osslan Osiris Vergara Villegas and ,
  • Gerardo Reyes Salgado and
  • Andrea Magadán Salazar
  • + 1 author+ 0 author

18 May 2024

In the realm of digital libraries, efficiently managing and accessing scientific publications necessitates automated bibliographic reference segmentation. This study addresses the challenge of accurately segmenting bibliographic references, a task co...

  • Article
  • Open Access
2 Citations
2,057 Views
16 Pages

EF1α and αTUB Are Stable Reference Gene Pairs for RT-qPCR-Based Gene Expression Studies in Salix suchowensis Under Nitrogen Treatment Conditions

  • Lei Huang,
  • Yuyi Zhang,
  • Fei Gao,
  • Yu Fu and ,
  • Jing Sun,
  • Jie Zhou,
  • Jun Tao,
  • Xudong He and
  • Nan Guo
  • + 4 authors+ 0 author

8 October 2025

Salix suchowensis is an ideal model organism for investigating nitrogen (N) transport mechanisms due to its low N-input requirements. Accurate quantification of gene expression is essential for elucidating these processes, with quantitative real-time...

(This article belongs to the Section Plant Molecular Biology)
  • Article
  • Open Access

An Integrated Assessment of Life-History Traits and Biological Reference Points of Oreochromis niloticus in Lake Nubia, Sudan

  • Alaa M. Younis,
  • Khaled Y. AbouelFadl,
  • Ebtesam A. A. A. AbouDeaf,
  • Mahmoud A. H. Kassem and ,
  • Manar Abdellatif,
  • Kassem A. S. Mohammed and
  • Alaa G. M. Osman
  • + 2 authors+ 0 author

10 November 2026

Contemporary biological information on Nile tilapia in Lake Nubia, Sudan, remains limited, which constrains evidence-based fishery management. This study provides an integrated assessment of life-history traits and biological reference points for Ore...

(This article belongs to the Section Marine and Freshwater Biology)

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