Diversity

Millions of animals are killed on roads annually due to collisions with vehicles, particularly medium–large mammals. Studies on mammal road-kill flourished in Brazil in the last decade and an assessment of research on road-kill impacts at a country level will help define science-based conservation strategies. In this study, we used the compiled scientific literature to provide the state of knowledge on medium and large-sized mammals killed by road traffic in Brazil, their conservation status, and an approximation of the road-kill magnitude. We reviewed a total of 62 scientific papers that reported road-kill data accounting for 11.817 individuals. Of the 102 species of medium–large mammals found in the IUCN list, more than half (n = 62; 61%) were recorded as road-kill on Brazilian roads. The Carnivora order comprises over a quarter (n = 23; 37%) of the total road-killed species. A total of 9 species (14.5%) were classified as threatened, with a further 10 (16%) as Near Threatened. Over half of the road-killed species (n = 33, 53%) showed declining population trends according to their IUCN conservation status. Our extrapolation of the results for the entire Brazilian paved road network showed that the number of medium–large road-killed mammals can reach almost 9 million yearly (maximum 8.7 million; mean 1.3 million), representing a biomass of more than 10000 tons. The highest roadkill rates were recorded for common generalists and least concern species, although there were also threatened and near threatened species within the top 15 highest road-kill rates. The declining population trends found for most species reflect serious conservation concerns, since there is a lack of information on the mortality effects at population levels. Our results suggest that medium–large mammals are severally affected by road mortality in Brazil. More investigations are needed at local and abundance population levels, in a way that allows the inclusion of road network as an important threat for target species impacted by road-kill in the national territory, in order to develop adequate plans to mitigate those impacts. Full article

The protection of wetlands is a cornerstone in the conservation of pond-breeding amphibians. Because protected wetlands are rarely natural areas, but are often man-made, at least in Europe, it is important that they are well managed to fulfill their intended function. Appropriate management requires knowledge of the ecology of the species, particularly habitat requirements. Here, we combine species monitoring data and habitat mapping data in an analysis where our goal was to describe the factors that determine the occupancy of amphibian species in federally protected amphibian breeding sites. As expected, every species had its own habitat requirements, often a combination of both a terrestrial and aquatic habitat (i.e., landscape complementation). In most species, occupancy was strongly positively affected with the amount of aquatic habitat, but predicted occupancy probabilities were low because the amount of aquatic habitat was low in most sites. The area or proportion of ruderal vegetation also had positive effects on multiple species, while other types of terrestrial habitat (e.g., meadows) led to low occupancy probabilities. The total area of the protected breeding sites was never included in a final model and connectivity was important only for one species (Triturus cristatus). The latter finding implies that the quality of the landscape between breeding sizes is more important than distance per se, while the former implies that the area of some specific habitats within breeding sites is crucial for high occupancies. Thus, increasing the amount of aquatic habitats and likewise terrestrial habitats within protected areas would make them more likely to achieve their conservation objectives. Our study is an example of how the joint analysis of monitoring data and habitat data (based on mapping in the field) can lead to evidence-based suggestions on how to improve conservation practice. Full article

A survey of the macrozoobenthos communities in the Maritsa River (South Bulgaria) was carried out in the summer of 2021. Benthic samples were collected and physicochemical parameters (water temperature, pH, conductivity, dissolved oxygen and nutrients) were measured at 15 sites located on the main river and its tributary system. The studied sites belonged to different river types and characterised the diversity of the ecological conditions—from unaffected to anthropogenically influenced river stretches. In addition, data from a study conducted in the summer of 2020 were used to analyse species–factor interactions in the river ecosystems and to assess the bio-indicative potential of the aquatic invertebrates. The dynamics of the taxonomic composition and abundance of the macrozoobenthos were analysed in relation to environmental factors. The physicochemical conditions of the water environment changed during the period of high water, which led to a reduction in the composition of the macrozoobenthos. Plecoptera and Trichoptera decreased in richness and abundance downstream and under human impacts. Ephemeroptera and Chironomidae were permanently present along the whole river. Oligochaeta increased in the lower river reaches and at sites with a greater amount of organic matter. The ecological status determined by the macrozoobenthos varied from high (site 1) to good, moderate and bad (site 13) at the studied sites. Full article

Enclosure (prohibition of grazing) is an important process to restore alpine grassland on the Qinghai-Tibetan Plateau. However, few studies have quantified the extent to which the long-term enclosure may contribute to the changes in plant phylogenetic diversity and community assembly in alpine grassland under environmental change. In this study, based on an 11-year fencing experiment along an altitudinal gradient ranging from 4400 m to 5200 m in central Tibet, we conducted an observation of species composition and coverage within and outside the fences in the fifth, eighth and eleventh year, and monitored the related climate and soil factors at 7 sites. Our aim is to quantify the relative effects of environmental change and grassland management on the alpine plant community assemblage. The results were: (1) the overall phylogenetic structure (NRI) of the alpine plant communities, whether inside or outside the enclosure, was divergent at altitudes where the environment was relatively unextreme (4800–5100 m), but aggregative at altitudes with low precipitation (4400–4650 m) or with low temperature (5200 m). (2) The phylogenetic structure of the nearest taxon of species (NTI) was more aggregative along the whole gradient. (3) Precipitation was the dominant factor driving the changes in species richness, phylogenetic diversity and community α-phylogenetic structure indices (NRI and NTI), followed by enclosure duration and soil C:N ratio. (4) The phylogenetic structure of the communities was similar at higher altitudes under grazing or enclosure treatments, and was opposite at lower sites. Stochastic processes have driven the changes in the communities between inside and outside the fences at all altitudes. In addition, homogeneous dispersal occurred in communities at higher sites. In summary, the 11-year enclosure had little effect on community structure of alpine meadows where the grazing pressure is relative lower, whereas it could help restore the community of steppe meadow at lower altitudes where the grazing pressure is extensively higher. This study may provide a vital theoretical support for the formulation of differential management for alpine grassland on the Tibetan Plateau. Full article

The Guadalquivir River Basin is one of the largest in the Iberian Peninsula and has a remarkable freshwater biodiversity. Although many studies on hydrological regimes or water quality have been conducted in this basin the biodiversity of freshwater fish, as well as their distribution and conservation status, has never been globally addressed as in other Iberian basins. In this context, we synthesized information on freshwater fish using field procedures and a bibliographic search. Fish distribution patterns at different spatial scales and general environmental conditions were analyzed as well as the conservation status of the fish community. We documented the presence of 40 species (20 native and 20 exotic) in the basin during the 20th century until today. However, we only captured 18 species during the field sampling, with a prevalence for any native species of less than 23% (except Luciobarbus sclateri). The highest species richness was found in mid reaches, while the lower reaches had very low diversity values. Around 50% of species are threatened; according to the IUCN, several species are declining at an alarming rate and others are probably extinct and/or their current status is unknown. Human disturbances during the last few decades have caused serious changes in fish distribution and consequently to their conservation status. Hydrological alterations, intensive agriculture and introduced species are probably the principal reasons for Guadalquivir’s ichthyofauna imperilment. Our study indicates an urgent and real need to identify important areas for fish conservation to guarantee a minimum fish biodiversity conservation over the long term, as well as effective strategies for fish recovery where it still is possible. Full article

Many aquatic species have restricted dispersal capabilities, making them the most vulnerable organisms to climate change and land use change patterns. These factors deplete Nymphaea species’ suitable habitats, threatening their populations and survival. In addition, the species are poorly documented, which may indicate how scarce they are or will become. Members of Nymphaea are ecologically important as well as having cultural and economic value, making them of conservation interest. Therefore, using the maximum entropy (MaxEnt) approach, climatic variables, land use, and presence points were modeled for seven Nymphaea species in South America, using three general circulation models (CCSM4, HADGEM2-AO, and MIROC5) and in two representative concentration pathways (RCPs 4.5 and 8.5) and two scenarios (2050 and 2070). Our results indicated that mean diurnal range (bio2), precipitation of the wettest month (bio13), temperature seasonality (bio15), and land use (dom_lu) were the main influencing factors. For all species, suitable areas were concentrated east of Brazil, and they were variable in northern parts of the continent. Besides, inconsistent expansion and contraction of suitable habitats were noticed among the species. For example, N. amazonum, N. rudgeana, and N. lasiophylla future habitat expansions declined and habitat contraction increased, while for N. ampla and N. jamesoniana, both future habitat expansion and contraction increased, and for N. pulchella and N. rudgeana it varied in the RCPs. Moreover, the largest projected suitable habitats were projected outside protected areas, characterized by high human impacts, despite our analysis indicating no significant change between protected and unprotected areas in suitable habitat change. Finally, understanding how climate change and land use affect species distribution is critical to developing conservation measures for aquatic species. Full article

Since 1996, the Adriatic sturgeon (Acipenser naccarii) has been inscribed on the IUCN Red List as “Critically Endangered and possibly extinct in the wild”. Nowadays, its survival totally depends on restocking programs conducted by releasing juveniles generated from adult breeders reared in aquaculture. Conducting accurate genetic characterizations of all individuals potentially involved in reproduction activities is therefore of primary importance to avoid inbreeding and to maximize the genetic diversity transmitted to following generations. Since all animals reared in captivity descend from a single stock of wild origin, this offers the ideal condition for carrying out relatedness analysis based on parentage allocations. In this study, we provided the most complete characterization of about 500 individuals representing the most diverse extant stock of Adriatic sturgeon. Through the analyses of mitochondrial d-loop and 15 microsatellite loci selected from 24 genotyped loci, we identified about 30 different familiar groups, updating data on breeding stocks, increasing the genetic information already available, and extending the analyses to animals never genotyped before. Given its completeness, it will represent a reference database for any future parental allocation of recaptured animals for the inclusion of all other stocks present, as well as for the development of a long-term breeding plan. The approach used has also been proven useful on individuals of unknown genealogy, allowing for the identification of family groups and thus being proven to be promising for the analysis of stocks of other tetraploid sturgeon species. Full article

Evolutionary mechanisms have shaped the genomic architecture of Colombian Creole cattle breeds. The mating and selection processes have impacted several traits, promoting differences within and between populations. Studies of population structure and selection signatures in Colombian Creole breeds are scarce, and need more attention to better understand genetic differentiation, gene flow, and genetic distance. This study aimed to analyze the population structure and identify selection imprints in the Criollo Caqueteño (CAQ) population. It used 127 CAQ animals genotyped with Chip HD 777,000 SNPs. The population structure analyses used discriminant principal component analysis (DAPC), integrated haplotype scoring (iHS), and index-fixing (Fst) methodologies to detect selection signals. We can highlight SNP regions on the genes TMPRSS15, PGAM2, and EGFR, identified by the Fst method. Additionally, the iHS regions for cluster 1 identified candidate genes on BTA 3 (CMPK1 and FOXD2), BTA 11 (RCAN1), and BTA 22 (ARPP21). In group 2, we can highlight the genes on BTA 4 (SLC13A4, BRAF), BTA 9 (ULBP), BTA 14 (CSMD3) and BTA 19 (KRTAP9-2). These candidate genes have been associated with fertility traits, precocity, growth, and environmental and disease resistance, indicating a genetic potential in CAQ animals. All this promotes a better understanding of the diversity and genetic structure in the CAQ population. Based on that, our study can significantly assist the sustainable development and conservation of the breed in the Colombian Amazon. Full article

The purpose of the present study was to perform bioinformatic analysis of crystallin diversity in aquatic molluscs based on the sequences in the NCBI Protein database. The objectives were as follows: (1) analysis of some physical and chemical properties of mollusc crystallins, (2) comparison of mollusc crystallins with zebrafish and cubomedusa Tripedalia cystophora crystallins, and (3) determination of the most probable candidates for the role of gastropod eye crystallins. The calculated average GRAVY values revealed that the majority of the seven crystallin groups, except for μ- and ζ-crystallins, were hydrophilic proteins. The predominant predicted secondary structures of the crystallins in most cases were α-helices and coils. The highest values of refractive index increment (dn/dc) were typical for crystallins of aquatic organisms with known lens protein composition (zebrafish, cubomedusa, and octopuses) and for S-crystallin of Pomacea canaliculata. The evolutionary relationships between the studied crystallins, obtained from multiple sequence alignments using Clustal Omega and MUSCLE, and the normalized conservation index, calculated by Mirny, showed that the most conservative proteins were Ω-crystallins but the most diverse were S-crystallins. The phylogenetic analysis of crystallin was generally consistent with modern mollusc taxonomy. Thus, α- and S-, and, possibly, J1A-crystallins, can be assumed to be the most likely candidates for the role of gastropod lens crystallins. Full article

The diversity of red algae is significantly increasing; in the recent interest of climate changes, taxonomic and biogeographic studies are needed in untouched regions such as ecological important islands. We examined a collection of Gelidium specimens gathered during the 2010 Atimo Vatae expedition based on the morphology and DNA sequencing of mitochondrial cox1 and plastid rbcL. Both morphological and molecular datasets demonstrated the presence of three species in Madagascar; G. leptum G.H.Boo, L.Le Gall and H.S.Yoon, sp. nov., described here, and G. sclerophyllum W.R.Taylor and G. usmanghanii Afaq-Husain and Shameel. Gelidium leptum is distinguished by thin, slender, flattened thalli with irregular branches, compactly arranged thick-walled cells in medulla, sparse rhizoidal filaments at both sides of branches, and elongate sori of tetrasporangia. Gelidium sclerophyllum, previously considered as endemic to the Tropical Eastern Pacific, is first reported in the Indian Ocean and Hawai’i; G. usmanghanii, previously reported to occur in Pakistan and Oman, also presents a first record in the Southern Hemisphere. Phylogenies inferred from cox1 and rbcL sequences suggests that each of the three species likely diverged from different ancestors. The Madagascan Gelidium comprises two geographical elements; Madagascan endemic (G. leptum) and widespread species (G. sclerophyllum and G. usmanghanii). Full article

(1) Background: Coleoptera is one of the most diverse insect lineages. Coleoptera species live in many ecosystems around the globe and their role in ecosystems is very diverse. To study the number and distribution of species, lists of species were compiled and then added into the database generation platforms. The aim of the work was to describe the modern fauna of Coleoptera based on a recently published dataset. (2) Methods: Studies were conducted from 1971 to 2022. Insects were collected by different means (searching under the bark of trees and stumps, sifting litter and wood dust, soil samples, caught in the light, in soil traps, window traps, etc.). For each observation, the coordinates of the place of discovery, the number of individuals, dates were noted. (3) Results: The dataset presents data on 2416 species and subspecies of Coleoptera from 89 families found in the Lipetsk region. In total, the number of studied specimens in the dataset was 16,184, the number of occurrences was 6192. The largest families in terms of species diversity were Staphylinidae (541 species), Curculionidae (416), Chrysomelidae (315) and Carabidae (285). (4) Conclusions: In addition, based on the analysis of additional references, 452 more species and 2 families are indicated. Thus, the biodiversity of Coleoptera of the Lipetsk region is 2868 species from 89 families. Full article

Many stakeholders, from governments to civil society to businesses, lack the data they need to make informed decisions on biodiversity, jeopardising efforts to conserve, restore and sustainably manage nature. Here we review the importance of enhancing biodiversity monitoring, assess the challenges involved and identify potential solutions. Capacity for biodiversity monitoring needs to be enhanced urgently, especially in poorer, high-biodiversity countries where data gaps are disproportionately high. Modern tools and technologies, including remote sensing, bioacoustics and environmental DNA, should be used at larger scales to fill taxonomic and geographic data gaps, especially in the tropics, in marine and freshwater biomes, and for plants, fungi and invertebrates. Stakeholders need to follow best monitoring practices, adopting appropriate indicators and using counterfactual approaches to measure and attribute outcomes and impacts. Data should be made openly and freely available. Companies need to invest in collecting the data required to enhance sustainability in their operations and supply chains. With governments soon to commit to the post-2020 global biodiversity framework, the time is right to make a concerted push on monitoring. However, action at scale is needed now if we are to enhance results-based management adequately to conserve the biodiversity and ecosystem services we all depend on. Full article

The ecology of functional features highlights the importance of the leaf economic spectrum (LES) in understanding plant trade-offs between conservative and commercial resource use. However, it is still unclear whether changes in the plant attributes of various vegetative organs can be altered and whether the plant economic spectrum (PES) is categorized by multiple vegetative organs. We investigated a total of 12 functional features of 174 woody tree species, with leaf and stem attributes, on Hainan Island. We used principal component analysis (PCA) to analyze the changes in attributes and connections to understand how the plant trade-offs differ. We detected that stem organic matter (SOM) and stem organic carbon (SOC) contributed most to the first principal component, followed by leaf organic matter (LOM) and leaf organic carbon (LOC). Using Spearman correlation analysis, we determined that leaf total nitrogen (LTN) and specific leaf area (SLA), LTN and leaf total phosphorus (LTP), and finally stem total nitrogen (STN) and stem total phosphorus (STP) were positively significantly correlated. These significant variations in the traits of nutrients are regulated, while the morphological traits of aboveground vegetative organs are diverse. The coexistence of species and community assembly can increase our knowledge on the tropical coastal secondary forests. Furthermore, our outcomes can help us to better understand the restoration of habitats and green infrastructure design, suggesting that selecting different species across multiple trait axes can help ensure functionality at the maximum level. Full article

The family Lecithoceridae (Lepidoptera, Gelechioidea) is one of the more poorly known groups of microlepidoptera, even though it is a highly diverse group containing more than 1430 known species so far. During the last two decades since the end of the 20th century, the author has intensively studied the family Lecithoceridae, especially for the less explored areas including southern Asia and the Oceanian and Afrotropical regions. Throughout these studies, the author (often with his co-authors) described the new subfamily Crocanthinae Park, 2015, 32 new genera, and over 540 new species of the family. In this article, a historical review for the taxonomic works of the family since the comprehensive study by Gozmány is briefly summarized, with a comparison of the number of genera/species of each subfamily, and the number of the species by the subfamily in each region of the world. All available information on the phylogeny, biology, morphological characters of subfamilies, and zoogeographical remarks for the distribution of family are listed and discussed. Full article

Resource utilization strategies of avian migrants are a major concern for conservation and management. Understanding seasonal habitat selection by migratory birds helps us explain the ongoing continental declines of migratory bird populations. Our objective was to compare the second-order and third-order habitat selection by the American White Pelican (Pelecanus erythrorhynchos; hereafter pelican) between the breeding and non-breeding grounds. We tested the Lack hypothesis that habitat selection by migratory birds is stronger on the breeding grounds than on the non-breeding grounds. We used random-effect Dirichlet-multinomial models to estimate the second-order habitat selection between the seasons with the GPS locations of 32 tracked pelicans. We used Gaussian Markov random field models to estimate the third-order habitat selection by pelicans at the breeding and non-breeding grounds, accounting for spatial autocorrelation. Pelicans strongly selected waterbodies and wetlands at both non-breeding and breeding grounds, tracking their foraging habitats between the seasons at the home range level. However, pelicans exhibited seasonal differences in the strength of the third-order selection of wetlands and waterbodies with foraging habitat selection being stronger at the breeding grounds than at the non-breeding grounds, supporting the Lack hypothesis. Full article

The phylogeography of the filed vole (Microtus agrestis) in Eurasia was thoroughly examined using mitochondrial DNA (mtDNA) of the cytochrome b (cytb) gene. However, the former conclusions about genetic variability and the contact zone of eastern and western genetic lineages in Lithuania were based on the analysis of a very limited number of individuals. In the present study, we examined 74 M. agrestis individuals trapped in four sites in the eastern, northern, and western parts of the country using sequence analysis of cytb and D-loop. Totals of 25 new cytb haplotypes and 19 new D-loop haplotypes were identified for this species. Higher nucleotide diversity was observed for D-loop (π = 0.01147 ± 0.00070) as compared to cytb (π = 0.00694 ± 0.00039). The phylogenetic analysis based on both loci revealed the presence of two genetic lineages, i.e., the eastern and western ones, which were mixed in Lithuanian samples, with the exception of the Rusnė site in the west of the country. Only the western lineage was observed in this island population of M. agrestis; the sample differed in low genetic variability and genetic differentiation from other investigated samples. We found D-loop to be an appropriate locus for the evaluation of the genetic variability of M. agrestis. Full article

In response to the present ecological crisis, new approaches to environmental conservation and management are being developed in Europe. One of the axes considered by nature protection stakeholders since the 2000s is to encourage the return of the wilderness. This has led to the deployment of a variety of initiatives, mainly led by civil and non-profit organisations. The objective here, through the analysis of the discourses of stakeholders—from semi-directive interviews—and the initiatives developed in Western Europe, is to understand how the multitude of projects carried out throughout Europe constitutes a proposal for new environmental management practices. Indeed, the studied initiatives introduce a paradigm shift by reflecting a will to go beyond the mere preservation of nature in order to contribute to a global territorial transition. To this end, the studied projects propose to create both a social and interspecies link around their sites, but also to ensure the development of virtuous economic forms in the territories. Full article

The frequency and intensity of hurricanes have increased with climate change, and their effects on most taxa are not known. We analyzed a species diversity of bats in three locations with different regimes of anthropic disturbance. We assessed the effect of the season and post-hurricane time on the abundance of trophic guilds in coastal Jalisco, México, during the two years following Hurricane Patricia (category 4). During a sampling effort of 15,629.76 m² of netting, we captured 790 bats of 21 species. The species diversity was higher in the site with the highest proportion of primary tropical deciduous forest and was higher in 2016 than in 2017; the species composition did not differ greatly between the two years. The abundance of bats in various trophic guilds varied relative to the four climatic seasons. The general abundance of bats, frugivores-omnivores, and insectivores showed a significant increasing trend over time after the hurricane, which may indicate a recovery of the ecosystem or an abundance of early-successional fruiting plants. The results also confirm that species diversity recovers faster in a conserved forest. Thus, it is important to conserve natural areas to mitigate the effects of major disturbances. Full article

The fatty acid profile of seven different organs (brain, gills, heart, muscles, gonads, liver, and gall bladder) of the lipid-rich Antarctic silverfish, Pleuragramma antarcticum, from the Antarctic Sound was studied. Qualitative and quantitative analyses of fatty acids were performed using gas chromatography with mass-selective and flame ionization detectors. It was seen that the muscles and gills differed from the other organs with the prevalence of saturated fatty acids, mainly due to 16:0 and 14:0 that may reveal their additional metabolic role in these organs for adaptation of the fish to the cold Antarctic waters. Monounsaturated fatty acids 18:1(n−-9) and 16:1(n−7) were prevalent in the other organs of silverfish, especially their high content in the liver, reflecting the carnivorous feeding type (mainly adult ice krill) of the species and their dietary preferences in the austral summer. The high content of essential fatty acids, 22:6(n−3) (14% of total fatty acids) found in the brain and 20:5(n−3) (12% of total fatty acids) found in the muscles, highlights the functional significance of these lipid components in the studied tissues of fish. The results will improve the understanding of the functional value of individual fatty acids in terms of their organ specificity and the adaptation of the Antarctic silverfish to life in the frigid ice waters of the Antarctic pelagic ecosystem. Full article