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18 pages, 2573 KB  
Article
Hyperspectral Response and Quantitative Determination of Protein in Complex Semi-Fluid Matrices: A Case Study of Highly Viscous Royal Jelly
by Fansong Zeng, Sheng Hu, Huimin Fang, Shihao Guan, Muhammad Hassan and Chao Zhao
Sustainability 2026, 18(18), 9382; https://doi.org/10.3390/su18189382 (registering DOI) - 12 Sep 2026
Abstract
Protein content is one of the most important indicators for evaluating the nutritional value and quality grade of royal jelly. To achieve rapid and non-destructive quantification of protein content in royal jelly, this study employed near-infrared hyperspectral imaging (NIR-HSI) to acquire hyperspectral images [...] Read more.
Protein content is one of the most important indicators for evaluating the nutritional value and quality grade of royal jelly. To achieve rapid and non-destructive quantification of protein content in royal jelly, this study employed near-infrared hyperspectral imaging (NIR-HSI) to acquire hyperspectral images of royal jelly samples, while the Kjeldahl method was simultaneously used as the reference method for protein determination. During data processing, seven spectral preprocessing methods—including the first derivative (1-Der), the second derivative (2-Der), Savitzky–Golay smoothing (SG), normalization (Normalize), baseline correction (Baseline), standard normal variate (SNV), and multiplicative scatter correction (MSC)—were comparatively evaluated. After outlier samples were identified and removed using the Mahalanobis distance method, Principal Component Regression (PCR) and Partial Least Squares Regression (PLSR) models were established for quantitative prediction of protein content in royal jelly. The PLSR models consistently outperformed the PCR models in predicting protein content in royal jelly. Among all preprocessing methods, the PLSR model developed using 1-Der spectra processing exhibited the best predictive performance, with a determination coefficient of calibration set () of 0.94, a determination coefficient of cross-validation set () of 0.90, a determination coefficient of prediction set () of 0.97, and a root mean square error of prediction (RMSEP) of 0.31%. These results support the feasibility of rapid and non-destructive laboratory-scale screening of protein content in royal jelly. The proposed method circumvents the drawbacks of conventional physicochemical analyses, which are labor-intensive, time-consuming and sample-destructive, and provides a basis for rapid and non-destructive laboratory-scale screening of protein content in royal jelly. Full article
(This article belongs to the Special Issue Sustainable Agricultural Engineering Technology and Development)
27 pages, 9486 KB  
Article
Groundwater-Depth Forecasting to Support Irrigation Management in the Tagus Vulnerable Zone Using an ARX–XGBoost Framework
by Diogo Pinto, Manuel Campagnolo, Maria João Martins, João Rolim, Beatriz Vacas and Maria do Rosário Cameira
Agriculture 2026, 16(18), 1959; https://doi.org/10.3390/agriculture16181959 (registering DOI) - 12 Sep 2026
Abstract
Groundwater is an essential source of irrigation water in Mediterranean agricultural regions, where seasonal crop water requirements and recurrent drought increase pressure on shallow aquifers. Forecasting groundwater depth can help irrigators and water managers anticipate changes in pumping conditions and identify periods of [...] Read more.
Groundwater is an essential source of irrigation water in Mediterranean agricultural regions, where seasonal crop water requirements and recurrent drought increase pressure on shallow aquifers. Forecasting groundwater depth can help irrigators and water managers anticipate changes in pumping conditions and identify periods of increased abstraction risk. This study presents a data-driven framework for forecasting groundwater depth in the Tagus Nitrate Vulnerable Zone, central Portugal, an intensively cultivated region substantially dependent on shallow alluvial groundwater. The framework combines an autoregressive model with exogenous inputs and extreme gradient boosting and applies a leakage-safe rolling-origin validation strategy. Groundwater depth was modelled independently at each monitoring well using monthly observations, accounting for data gaps and uneven record lengths. Performance was assessed over forecast horizons of up to 12 months using error metrics calculated only from observed values. Feature-importance analysis showed the dominant role of groundwater persistence and seasonality, together with site-dependent contributions from precipitation, reference evapotranspiration, and river discharge. Forecast errors increased gradually with lead time but remained below 1 m for 14 of 17 wells. The framework provides forward-looking information that can complement irrigation-demand assessment and groundwater monitoring, supporting the anticipation of changing pumping conditions and the identification of areas requiring closer abstraction management. Full article
(This article belongs to the Section Agricultural Water Management)
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34 pages, 10872 KB  
Systematic Review
Time-Zero Kidney Biopsy and Deceased-Donor Transplant Outcomes: A Systematic Review and Meta-Analysis of Prognostic Value, Prediction, and Clinical Utility
by Livier Sainz-Bravo, Benjamín Gómez-Navarro, Diana Laura Figueroa-Gamiño, Jenniffer Topacio Tapia-Báez, José David González-Barajas, Manuel Luis Prieto-Magallanes, José Ángel Solís-Rojas, Edgar Bautista-Reyes, Juan Alberto Gómez-Fregoso, Judith Carolina De Arcos-Jiménez, Ramón Medina-González, Violeta Aidee Camarena-Arteaga, Manuel Arizaga-Napoles and Jaime Briseno-Ramirez
Med. Sci. 2026, 14(5), 566; https://doi.org/10.3390/medsci14050566 (registering DOI) - 12 Sep 2026
Abstract
Background/Objectives: Time-zero biopsy informs deceased-donor kidney implantation or discard, but its prognostic value is contested. We systematically reviewed pre-reperfusion morphological biopsy evidence in adult deceased-donor kidney transplantation, keeping four questions separate: prognostic association, incremental prediction, clinical utility and measurement reproducibility. Methods: We searched [...] Read more.
Background/Objectives: Time-zero biopsy informs deceased-donor kidney implantation or discard, but its prognostic value is contested. We systematically reviewed pre-reperfusion morphological biopsy evidence in adult deceased-donor kidney transplantation, keeping four questions separate: prognostic association, incremental prediction, clinical utility and measurement reproducibility. Methods: We searched PubMed/MEDLINE, Embase, Scopus, Web of Science, CENTRAL and three trial registries through 25 July 2026 without date or language restriction. One estimate per non-overlapping cohort was selected under a prespecified hierarchy; random-effects models used restricted maximum likelihood with Hartung–Knapp inference; GRADE assessed certainty. Results: Of 45,676 records, 25,746 were screened; 203 eligible reports (159 study investigations and 154 cohort families) and 22 contextual reports were included. Vascular injury was associated with delayed graft function (odds ratio: 1.85, 95% CI 1.11–3.06), glomerulosclerosis with graft failure (hazard ratio: 2.03, 1.22–3.39) and vascular injury with graft failure (hazard ratio: 1.48, 1.07–2.04); glomerulosclerosis with delayed graft function was inconclusive (odds ratio: 1.13, 0.59–2.17). All 12 pooled bodies were of very low certainty. Conclusions: Current evidence does not support time-zero histology as a stand-alone rule for accepting or discarding a deceased-donor kidney. Whether standardized histology adds predictive information beyond established clinical variables remains open and should be addressed in linked registry analyses before biopsy findings guide management. Full article
(This article belongs to the Section Nephrology and Urology)
33 pages, 1874 KB  
Article
The Role of Tourism Villages: Contextual Support for Sustainable Livelihoods Based on Destination Characteristics
by Endy Marlina, Annisa Mu’awanah Sukmawati, Ratika Tulus Wahyuhana and Hasto Andreawan
Tour. Hosp. 2026, 7(9), 297; https://doi.org/10.3390/tourhosp7090297 (registering DOI) - 12 Sep 2026
Abstract
This study introduces the concept of contextuality in sustainable livelihood support, shaped by destination characteristics. In cultural tourism, support is most pronounced for human and social capital, reflecting the importance of knowledge, skills, creativity, cultural identity, community participation, and local institutions. In nature-based [...] Read more.
This study introduces the concept of contextuality in sustainable livelihood support, shaped by destination characteristics. In cultural tourism, support is most pronounced for human and social capital, reflecting the importance of knowledge, skills, creativity, cultural identity, community participation, and local institutions. In nature-based tourism, support is most pronounced for physical, human, and natural capital, highlighting the importance of infrastructure, accessibility, safety, innovation, adaptability, and environmental conservation. Meanwhile, social capital serves as a vital social investment during times of crisis. The research evaluates five types of capital—human, social, financial, physical, and natural—using the Sustainable Livelihood Framework for Tourism (SLFT) to explore the role of tourism villages in supporting sustainable livelihoods. The study employed a sequential explanatory mixed-methods approach. It collected quantitative data from 140 local stakeholders using a 25-item Likert-scale questionnaire and gathered qualitative data through semi-structured interviews with 90 stakeholders to validate the quantitative findings. The study concludes that community-based tourism does not yield uniform livelihood patterns. To achieve sustainable livelihoods, tourism village policies must be formulated contextually, based on local assets, to strengthen community resilience, inclusivity, and sustainability. Full article
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37 pages, 7811 KB  
Article
VFR-MISE: An Asymmetric Dual-Evidence Verification–Fallback Framework for Few-Shot Stressor Extraction from Social Media
by Xiaohua Wang, Haiyang Yu and Fang Niu
Big Data Cogn. Comput. 2026, 10(9), 315; https://doi.org/10.3390/bdcc10090315 (registering DOI) - 12 Sep 2026
Abstract
Few-shot stressor extraction from temporally evolving social-media text remains challenging, primarily because of unstable span boundaries and the imperfect correspondence between structural confidence and task-semantic validity. We propose VFR-MISE, an asymmetric dual-evidence verification–fallback framework that extends the temporal few-shot knowledge-inheritance paradigm of MISE. [...] Read more.
Few-shot stressor extraction from temporally evolving social-media text remains challenging, primarily because of unstable span boundaries and the imperfect correspondence between structural confidence and task-semantic validity. We propose VFR-MISE, an asymmetric dual-evidence verification–fallback framework that extends the temporal few-shot knowledge-inheritance paradigm of MISE. The framework employs DeBERTa-v3-base for candidate generation, uses a guideline-conditioned verifier to assess candidate-level semantic validity, and applies BIOES emission evidence only to selectively recover high-confidence candidates rejected by the verifier. In a backbone comparison involving 20 random seeds, the DeBERTa-based candidate generator achieves a higher Exact-span F1 than its RoBERTa-based counterpart (0.7215 vs. 0.6942; +2.72 percentage points). The corresponding complete A3 systems achieve F1 scores of 0.7248 and 0.6977 (+2.70 percentage points; exact sign-flip test, p = 0.0000458). With the DeBERTa backbone fixed, the complete VFR-MISE framework improves the mean cross-shot Exact-span F1 by 0.33 percentage points over A0. Although this gain is modest, the overall results still provide directional support for the verification–fallback mechanism and indicate a relatively consistent refinement of the precision–recall trade-off. Computational cost analysis further shows that the full DeBERTa verifier introduces substantial additional computational overhead. In a matched benchmark, replacing the DeBERTa verifier with MiniLM reduces end-to-end inference time by 26.28% at a batch size of 16, suggesting that MiniLM is a promising candidate for lightweight verification. Overall, within the current experimental setting, VFR-MISE provides an auditable framework for stressor extraction that integrates semantic verification with constrained structural recovery. Full article
(This article belongs to the Special Issue Natural Language Processing and Text Analysis in Social Media)
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21 pages, 975 KB  
Article
A Low-Cost Platform for Measuring Environmental and Geographic Positioning Data in Unmanned Vehicles: A Device and Computing Integration Framework
by Gerardo Aguayo Núñez, Jorge Aurelio Brizuela Mendoza, Julio C. Rosas-Caro, Jesse Yoe Rumbo Morales, Abraham Jair López Villavazo, Alan Francisco Pérez Vidal and Gerardo Ortiz Torres
Eng 2026, 7(9), 473; https://doi.org/10.3390/eng7090473 (registering DOI) - 12 Sep 2026
Abstract
Unmanned vehicles require reliable embedded systems that can acquire, process, and transmit operational data in real time to support monitoring, navigation, and decision-making. This paper presents a modular Logic Computer architecture designed for telemetry and system supervision. The proposal integrates embedded hardware to [...] Read more.
Unmanned vehicles require reliable embedded systems that can acquire, process, and transmit operational data in real time to support monitoring, navigation, and decision-making. This paper presents a modular Logic Computer architecture designed for telemetry and system supervision. The proposal integrates embedded hardware to provide sensor acquisition, wireless telemetry transmission, and fault reporting within a lightweight, scalable architecture. Experimental results demonstrate the proposed framework’s effectiveness and suitability for unmanned vehicle applications. Furthermore, the results provide insight into data transmission analysis in terms of technical aspects such as latency, jitter, throughput, and packet loss within a low-power-consumption framework. Based on these metrics, a comparison with existing solutions is presented, highlighting differences, drawbacks, and advantages. Tests conducted in the field also provide environmental and geographic positioning data results to demonstrate performance. Consequently, the presented prototype offers strong scalability and performance advantages over current systems, driven by its open-source architecture and high-bandwidth data transmission. Full article
24 pages, 1400 KB  
Article
Structure–Activity Relationship Analysis of Immunoassay Systems for (Fluoro)quinolones Detection
by Platon P. Chebotaev, Andrey A. Buglak, Nadezhda A. Byzova, Anatoly V. Zherdev and Olga D. Hendrickson
Int. J. Mol. Sci. 2026, 27(18), 8140; https://doi.org/10.3390/ijms27188140 (registering DOI) - 12 Sep 2026
Abstract
Detection systems for antibiotics of the (fluoro)quinolone (FQ) group are in high demand among food safety regulators and agricultural inspectors. At the same time, the application of developed test systems may be complicated by the significant structural diversity of related FQs (including enantiomeric [...] Read more.
Detection systems for antibiotics of the (fluoro)quinolone (FQ) group are in high demand among food safety regulators and agricultural inspectors. At the same time, the application of developed test systems may be complicated by the significant structural diversity of related FQs (including enantiomeric forms), which necessitates the careful evaluation of their selectivity. In this study, five polyclonal antibodies were generated by immunizing rabbits with the S- and R-enantiomers of ofloxacin (OFL) and its racemic mixture used as haptens. The cross-reactivity (CR) of the obtained antibodies toward 26 FQs, structural analogs of OFL, was evaluated using an indirect competitive enzyme-linked immunosorbent assay. Structure–activity relationship models were developed to analyze the obtained CR data. Three machine learning (ML) methods were applied: random forest classifier (RFC), logistic regression (LR), and a support vector classifier (SVC). The SVC model demonstrated the highest predictive performance in terms of the Log Loss metric. In contrast, the LR models showed the best overall balance across six statistical metrics, including precision, recall, and F1 score. Three-dimensional topological descriptors enabled discrimination between the S- and R-isomers of OFL, whereas constitutional and two-dimensional descriptors were less effective. The obtained results contribute to the development of FQ immunoassay systems and their computational analysis using ML approaches. Full article
(This article belongs to the Special Issue Exploring Molecular Properties Through Molecular Modeling)
23 pages, 870 KB  
Article
Analytical and Clinical Performance of VIDAS® HIV DUO AG/AB Assay for Enhanced Early HIV Diagnosis
by Véronique Lemée, Mathilde Lacôte, Peggy Nomade, Yves Mérieux, Sandrine Gréaume, Isabelle Voisin, Laurence Bridon, Jean-Christophe Plantier, Elodie Alessandri Gradt and Vinca Icard
Diagnostics 2026, 16(18), 2954; https://doi.org/10.3390/diagnostics16182954 (registering DOI) - 12 Sep 2026
Abstract
Background/Objectives: Early detection of HIV infection is critical for timely clinical management and prevention of transmission. Fourth-generation antigen/antibody assays improve early diagnosis by detecting p24 antigen prior to seroconversion. This study evaluated the analytical and clinical performance of the VIDAS® HIV [...] Read more.
Background/Objectives: Early detection of HIV infection is critical for timely clinical management and prevention of transmission. Fourth-generation antigen/antibody assays improve early diagnosis by detecting p24 antigen prior to seroconversion. This study evaluated the analytical and clinical performance of the VIDAS® HIV DUO AG/AB assay. Methods: A multicenter evaluation was conducted using WHO HIV-1 p24 standards, 50 HIV-1/HIV-2 culture supernatants representing diverse genotypes, 40 seroconversion panels, 634 HIV-positive clinical specimens, 6063 HIV-negative samples from four populations, and 272 potentially cross-reactive samples. Precision was assessed according to CLSI EP05-A3 guidelines. Performance was evaluated against VIDAS® HIV DUO Ultra and contextualized with results reported for other fourth-generation assays. Results: VIDAS® HIV DUO AG/AB showed a lower p24 antigen limit of detection (0.3 IU/mL) than VIDAS® HIV DUO Ultra (0.5–0.6 IU/mL) and higher relative p24 reactivity across multiple genotypes. It enabled earlier detection in more than 30% of seroconversion panels, corresponding to a cumulative gain of 60 days versus VIDAS® HIV DUO Ultra. Based on historical datasets, gains of up to 29 days were observed relative to reported performances of the ARCHITECT HIV Ag/Ab Combo and MAGLUMI HIV Ab/Ag Combi assays. All 634 HIV-positive clinical samples were detected, demonstrating broad genotype inclusivity within the diversity of HIV strains represented in this study. Specificity reached 99.95% (95% CI: 99.90–99.99%), with no cross-reactivity observed. Precision coefficients of variation were below 3.5%. Conclusions: The VIDAS® HIV DUO AG/AB assay demonstrated high analytical sensitivity, early detection in seroconversion panels, broad genotype coverage, and excellent (>99.5%) specificity, supporting its use as a fourth-generation option for HIV screening in various clinical and epidemiological settings. Full article
(This article belongs to the Special Issue Innovations in HIV Diagnostics and Monitoring)
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21 pages, 4446 KB  
Article
AI-Enabled Smart Monitoring of Bovine Embryo Development Using Time-Lapse Imaging and Transfer Learning
by Manickavasagan Shivaani, Meenakshi P.L and Pavneesh Madan
Future Internet 2026, 18(9), 476; https://doi.org/10.3390/fi18090476 (registering DOI) - 12 Sep 2026
Abstract
Time-lapse incubation systems enable the continuous, non-invasive monitoring of embryonic development; however, identifying developmental stages still requires substantial manual assessment, making the process time-consuming, labor-intensive, and potentially subjective. This study evaluated the developmental kinetics of bovine embryos cultured in synthetic oviductal fluid (SOF) [...] Read more.
Time-lapse incubation systems enable the continuous, non-invasive monitoring of embryonic development; however, identifying developmental stages still requires substantial manual assessment, making the process time-consuming, labor-intensive, and potentially subjective. This study evaluated the developmental kinetics of bovine embryos cultured in synthetic oviductal fluid (SOF) and Gx-TL™ media using MIRI time-lapse imaging, and investigated the effectiveness of transfer learning-based convolutional neural networks (CNNs) for automated embryo-stage classification. A total of 311 zygotes were individually cultured under standard in vitro fertilization conditions, including 152 embryos in SOF medium and 159 embryos in Gx-TL™ medium. In the SOF group, 81 embryos reached the two-cell stage, 10 developed to the morula stage, and 6 reached the blastocyst stage. In the Gx-TL™ group, 77 embryos reached the two-cell stage, 18 developed to the morula stage, and 10 reached the blastocyst stage. Time-lapse images were manually annotated according to key developmental stages, including the two-cell through eight-cell stages, morula, and blastocyst. The annotated image dataset was augmented to 5000 images and used to train three pretrained CNN architectures: ResNet18, DenseNet121, and EfficientNet-B0. All three models achieved 100% accuracy in the two-class classification task across both culture media. Classification accuracy ranged from 95% to 100% for the nine-class model and from 98% to 100% for the ten-class model. These findings provide preliminary evidence that transfer learning-based convolutional neural networks (CNNs) can support the automated classification of bovine embryonic developmental stages using time-lapse images. Full article
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16 pages, 5451 KB  
Article
Prognostic Implications of Recurrence Patterns and Time to Recurrence in Post-Hepatectomy Hepatocellular Carcinoma Recurrence: An Exploratory Analysis
by Jianwei Chen, Wenhao Teng, Mingji Zhang, Xiaolong Wang, Yuemin Zhu, Liting Wen, Juyi Wu, Dechun Zheng and Zhuting Fang
Curr. Oncol. 2026, 33(9), 555; https://doi.org/10.3390/curroncol33090555 (registering DOI) - 12 Sep 2026
Abstract
Post-recurrence survival (PRS) after hepatectomy for hepatocellular carcinoma (HCC) varies widely, yet the prognostic significance of the recurrence pattern and time to recurrence (TTR) for PRS remains incompletely defined. This single-center retrospective study included 126 patients with first HCC recurrence after curative hepatectomy [...] Read more.
Post-recurrence survival (PRS) after hepatectomy for hepatocellular carcinoma (HCC) varies widely, yet the prognostic significance of the recurrence pattern and time to recurrence (TTR) for PRS remains incompletely defined. This single-center retrospective study included 126 patients with first HCC recurrence after curative hepatectomy (2018–2024) and aimed to explore the prognostic role of recurrence pattern, classified as low-risk (oligo-recurrence: ≤3 intrahepatic nodules, each ≤3 cm) or high-risk (multiple/large intrahepatic, tumor in vein, or extrahepatic), for PRS. In the overall cohort, the recurrence pattern independently predicted PRS (hazard ratio 2.13, p = 0.015); the preoperative Barcelona Clinic Liver Cancer (BCLC) stage, gamma-glutamyl transferase, and total bilirubin were also independent predictors. A sensitivity analysis, including post-recurrence treatment, attenuated the hazard ratio for high-risk pattern to 1.82 (95% CI 0.93–3.55, p = 0.079), consistent with partial mediation. Kaplan–Meier analyses suggested an association between shorter TTR and worse overall survival (OS), but this finding is descriptive because OS measured from surgery is mathematically coupled with TTR. In an exploratory subgroup analysis of patients with early-stage primary HCC (BCLC 0/A, n = 98), the recurrence pattern showed a borderline association with PRS (p = 0.053). Late detection was associated with high-risk recurrence but did not independently affect survival. These findings highlight the prognostic value of the recurrence pattern for PRS and support the consideration of TTR as a descriptive prognostic indicator, which together may inform risk-adapted follow-up and treatment strategies. Full article
(This article belongs to the Section Gastrointestinal Oncology)
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14 pages, 1110 KB  
Article
Temporal Changes in Diagnostic Composition and Treatment Activity Among Mechanically Ventilated ICU Patients Receiving Hyperbaric Oxygen Therapy: An 11-Year Single-Centre Retrospective Study
by Aneta Miszewska, Olga Sobczak, Piotr Góralczyk and Jacek Kot
J. Clin. Med. 2026, 15(18), 7089; https://doi.org/10.3390/jcm15187089 (registering DOI) - 12 Sep 2026
Abstract
Background: Mechanically ventilated intensive care patients receiving hyperbaric oxygen therapy (HBOT) are uncommon and resource-intensive. We assessed temporal changes over 11 years in diagnostic composition, HBOT treatment activity, organ-support requirements, and in-hospital mortality at an academic centre. Methods: This single-centre retrospective [...] Read more.
Background: Mechanically ventilated intensive care patients receiving hyperbaric oxygen therapy (HBOT) are uncommon and resource-intensive. We assessed temporal changes over 11 years in diagnostic composition, HBOT treatment activity, organ-support requirements, and in-hospital mortality at an academic centre. Methods: This single-centre retrospective cohort included mechanically ventilated ICU patients receiving HBOT during the period 2013–2023. Diagnoses were grouped as carbon monoxide (CO) poisoning, clostridial myonecrosis/gas gangrene, non-clostridial necrotising soft-tissue infections (NSTIs), or other indications. Annual patient and HBOT patient-session volumes and the annual number and proportion of nocturnal sessions were summarised descriptively. Calendar-time associations were estimated using exploratory univariable logistic regression with calendar year entered as a continuous predictor and are reported as odds ratios (ORs) per one-year increase with 95% confidence intervals (CIs). Results: Of 6410 HBOT-treated patients, 176 mechanically ventilated ICU patients (2.7%) underwent 1381 sessions; 781 (56.6%) were nocturnal. Annual volumes ranged from 10 to 22 patients and from 66 to 190 sessions, without a monotonic increase. The estimated OR per one-year increase was 0.85 (95% CI: 0.76–0.95) for CO poisoning, 0.85 (95% CI: 0.76–0.96) for clostridial myonecrosis/gas gangrene, 1.23 (95% CI: 1.11–1.36) for non-clostridial NSTIs, and 1.17 (95% CI: 1.04–1.31) for catecholamine infusion. Conclusions: Diagnostic composition shifted towards non-clostridial NSTIs. Catecholamine infusion was more frequent in the later calendar years, whereas the CVVHDF estimate was small and imprecise and the mortality estimate was near null. Persistent nocturnal activity supports ICU-capable infrastructure and flexible staffing for emergency HBOT. Full article
(This article belongs to the Section Intensive Care)
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28 pages, 1686 KB  
Article
Predicting Negative Day-Ahead Electricity Prices Across 12 European Bidding Zones: A Gate-Closure-Audited Explainable Machine Learning Framework
by Tomasz Rokicki, Piotr Bórawski, Aneta Bełdycka-Bórawska and Bogdan Klepacki
Appl. Sci. 2026, 16(18), 9063; https://doi.org/10.3390/app16189063 (registering DOI) - 12 Sep 2026
Abstract
The growing penetration of variable renewable energy (VRE) is increasing the frequency of very low and negative prices, although these events also depend on demand, transmission capacity, price-regime persistence and flexibility resources. This study examines which pre-auction and diagnostic variables are associated with [...] Read more.
The growing penetration of variable renewable energy (VRE) is increasing the frequency of very low and negative prices, although these events also depend on demand, transmission capacity, price-regime persistence and flexibility resources. This study examines which pre-auction and diagnostic variables are associated with negative day-ahead prices across European bidding zones, and whether these relationships remain stable over time and transferable across markets. More than 736,000 observations from 12 bidding zones in 2019–2025 were analysed, with sample coverage varying by data completeness. A gate-closure-audited Extreme Gradient Boosting (XGBoost) model achieved moderate risk-ranking performance and positive probabilistic skill in 2024–2025. The strongest pre-auction signals were completed-auction price history, calendar features and structural load relationships. Leave-one-market-out validation showed partial and heterogeneous transferability, supporting local calibration. A separate diagnostic layer revealed market-specific, non-linear associations involving VRE forecasts, residual load and cross-border exchange. Negative prices are therefore interpreted as screening signals for market configurations potentially associated with limited surplus absorption, rather than direct evidence of a flexibility shortfall. The framework separates operational pre-auction prediction from later market diagnosis and provides a reproducible basis for local early-warning applications. All SHAP, ALE and scenario results are interpreted as predictive associations and model diagnostics, not as causal effects or direct measures of physical flexibility. Full article
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21 pages, 2641 KB  
Article
Bio-Inspired Hyperparameter Optimization of LSTM Networks for Long-Horizon Stock Price Forecasting
by Manan Bhasin, Rajesh Mahadeva, Amit Kumar Goyal and Varun Sarda
Forecasting 2026, 8(5), 84; https://doi.org/10.3390/forecast8050084 (registering DOI) - 12 Sep 2026
Abstract
Accurate stock price forecasting remains a challenging problem because financial markets are highly dynamic, nonlinear, and influenced by changing economic conditions. Deep learning models, particularly Long Short-Term Memory (LSTM) networks, have shown strong capability in capturing temporal dependencies in financial time series. However, [...] Read more.
Accurate stock price forecasting remains a challenging problem because financial markets are highly dynamic, nonlinear, and influenced by changing economic conditions. Deep learning models, particularly Long Short-Term Memory (LSTM) networks, have shown strong capability in capturing temporal dependencies in financial time series. However, their performance is often sensitive to hyperparameter selection, which can affect convergence, stability, and generalization. This research presents a comparative forecasting framework using three bio-inspired hyperparameter optimization algorithms: Sand Cat Swarm Optimization (SCSO), Particle Swarm Optimization (PSO), and Grey Wolf Optimization (GWO). Each of the optimization algorithms works on a common hyperparameter search space comprising the learning rate, dropout rate, batch size and number of units in the LSTM layers, while the input sequence length is fixed at 60 trading days. The models are compared with a fixed-hyperparameter baseline LSTM to validate whether hyperparameter optimization improves price forecasting. The models are evaluated using daily data from five DAX-index equities over two sample periods: 2018–2023 and 1996–2024, respectively, with 2023 and 2019–2024 being their respective held-out test sets. The LSTM predicts the next-trading-day log return, which is transformed into a forecast of the next adjusted closing price. Historical prices and technical indicators representing trend, momentum, and volatility are incorporated into the modeling process. Chronological data splitting, training-only scaler fitting, validation-based hyperparameter selection, and a held-out test set used exclusively for final evaluation are employed to limit look-ahead bias, with every model evaluated across three random seeds. Model performance is assessed using Mean Absolute Error (MAE), Root Mean Squared Error (RMSE), Mean Absolute Percentage Error (MAPE), coefficient of determination (R2) and directional accuracy, along with support from Diebold–Mariano tests and Ljung–Box and ARCH residual diagnostics. LSTM–SCSO achieves the strongest aggregate error performance during 2018–2023, reducing mean RMSE by approximately 2.5% relative to the baseline. During 1996–2024, PSO–LSTM ranks first on RMSE, MAE, and MAPE, reducing mean RMSE by approximately 3.9%, while GWO–LSTM produces closely comparable results. Optimized models record the lowest equity-level mean RMSE for four of five equities in the short-term experiment and three of five equities in the long-term experiment. However, Diebold–Mariano significance varies across model–equity comparisons, and aggregate directional accuracy remains below 50% for all models in both periods. Long-term residual diagnostics also identify substantial autocorrelation and ARCH effects. The findings demonstrate that bio-inspired metaheuristic optimization can improve one-step-ahead LSTM forecasts, but its benefits depend on the optimizer, equity, and historical evaluation period. Full article
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21 pages, 2499 KB  
Article
Effect of Thermal Aging on the Self-Healing Properties of High-Content Crumb Rubber–SBS Composite Modified Asphalt
by Xiang Ma, Zitong Min, Chaolin Zhang, Yiwan Luo, Muneer K. Saeed and Ahmed D. Almutairi
Coatings 2026, 16(9), 1086; https://doi.org/10.3390/coatings16091086 (registering DOI) - 12 Sep 2026
Abstract
High-content crumb rubber composite modified asphalt (HRCMA), which combines crumb rubber with a styrene–butadiene–styrene (SBS) modifier, offers excellent mechanical performance and environmental benefits, yet its high viscosity requires elevated production and construction temperatures that may accelerate thermo-oxidative aging and impair its durability and [...] Read more.
High-content crumb rubber composite modified asphalt (HRCMA), which combines crumb rubber with a styrene–butadiene–styrene (SBS) modifier, offers excellent mechanical performance and environmental benefits, yet its high viscosity requires elevated production and construction temperatures that may accelerate thermo-oxidative aging and impair its durability and self-healing capability. In this study, the self-healing behavior of HRCMA under virgin, Thin Film Oven Test (TFOT)-aged, and Pressure Aging Vessel (PAV)-aged conditions was systematically investigated and compared with that of conventional SBS modified asphalt (SBSMA) using fatigue–healing–fatigue tests. The effects of damage degree, healing interval time, and healing temperature were evaluated, and a comprehensive self-healing index (HI) integrating the recovery of initial mechanical properties and the recovery of damage evolution characteristics was proposed. The results show that increasing the damage degree significantly reduces the self-healing capability of both binders, and thermal aging further intensifies this deterioration; two-way analysis of variance (ANOVA) confirmed that these effects are statistically significant. Extending the healing interval time and raising the healing temperature improve the self-healing performance, although the enhancement weakens after severe aging owing to the reduction in molecular mobility. Compared with SBSMA, HRCMA exhibits higher self-healing capability under all aging conditions, indicating stronger resistance to aging-induced damage. The proposed HI provides a more comprehensive evaluation of asphalt self-healing behavior, and the findings provide theoretical support for the durability assessment and engineering application of highly modified asphalt materials. Full article
(This article belongs to the Section Architectural and Infrastructure Coatings)
27 pages, 15905 KB  
Article
Multi-Season Multi-Model GWAS Prioritizes Stable Genomic Loci and Candidate Genes for Six Agronomic Traits in Soybean Under Conditions in Southeastern Kazakhstan
by Alibek Zatybekov, Yuliya Genievskaya, Chao Fang, Zixuan Wang, Svetlana Didorenko, Saule Abugalieva and Yerlan Turuspekov
Plants 2026, 15(18), 2800; https://doi.org/10.3390/plants15182800 (registering DOI) - 12 Sep 2026
Abstract
Reliable identification of genomic regions controlling complex agronomic traits across variable growing seasons remains a major challenge in soybean genetics and breeding. Here, a diverse panel of 252 soybean accessions was evaluated over six consecutive growing seasons (2018–2023) for flowering time, maturity, plant [...] Read more.
Reliable identification of genomic regions controlling complex agronomic traits across variable growing seasons remains a major challenge in soybean genetics and breeding. Here, a diverse panel of 252 soybean accessions was evaluated over six consecutive growing seasons (2018–2023) for flowering time, maturity, plant height, number of seeds per plant, seed yield per plant, and thousand-seed weight. Whole-genome resequencing and variant filtering yielded 2,019,772 high-quality SNPs, and association signals were evaluated using Inclusive Integrative Input Multiple-locus Random-SNP-effect Mixed Linear Model (IIIVmrMLM), Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK), and Multi-Locus Mixed Model (MLMM) together with linkage disequilibrium (LD)-based locus consolidation. Cross-model prioritization retained 21 high-confidence loci supported by all three GWAS models and distributed across 10 chromosomes. Among the identified loci, 18 overlapped or co-localized with previously reported SoyBase genes and QTLs, whereas three (q.VER2.13-1, q.YP.01-1, and q.TSW.15-1) showed no positional overlap with known genes and QTLs and were therefore considered presumably novel. These three loci were associated with flowering time, yield per plant, and thousand-seed weight, accounting for 1.60%, 2.13%, and 5.53% of phenotypic variation, respectively. Ten loci co-localized with genomic regions containing established soybean regulators, including E2, E3, GmDt2, and POWR1, support the biological plausibility of the association results. Integration of genomic position, functional annotation, and tissue-expression evidence prioritized 112 candidate genes across 18 loci. These findings provide a focused set of genomic loci and candidate genes for independent validation and further investigation of the genetic basis of soybean adaptation and yield formation under variable continental growing conditions. Full article
(This article belongs to the Special Issue Genetic Mapping of Agronomic Traits in Crops)
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