Stably Expressed Genes (SEGs) are a set of genes with invariant expression. Identification of SEGs, especially among both healthy and diseased tissues, is of clinical relevance to enable more accurate data integration, gene expression comparison and...
Through interactions between the modes of “waves” and “vortices”, stably stratified turbulence exhibits characteristic features both in 2D and 3D. Using DNS of the Navier–Stokes equations coupled to an equation for tempe...
We consider the separation of motion related to internal gravity waves and eddy dynamics in stably stratified flows obtained by direct numerical simulations. The waves’ dispersion relation links their angle of propagation to the vertical
A coordinated action between nuclear and mitochondrial activities is essential for a proper cellular response to genotoxic stress. Several nuclear transcription factors, including STAT3, translocate to mitochondria to exert mitochondrial function reg...
RT-qPCR is the gold standard and the most commonly used method for measuring gene expression. Selection of appropriate reference gene(s) for normalization is a crucial part of RT-qPCR experimental design, which allows accurate quantification and reli...
The growth hormone secretagogue receptor-1a (GHSR1a) is the endogenous receptor for ghrelin. Activation of GHSR1a participates in many physiological processes including energy homeostasis and eating behavior. Due to its transitory half-life, the effi...
The focus of the current study is on the anisotropy of stably stratified turbulence that is not only limited to large scales and an inertial subrange but also penetrates to small-scale turbulence in the viscous/dissipation subrange on the order of th...
A theorem of Sheshadri (Proc. Nat. Acad. Sci. USA 44 (1958) 456–458) shows that, when
WRINKLED1 (WRI1), an APETALA2/ethylene-responsive-element-binding protein (AP2/EREBP) subfamily transcription factor, plays a crucial role in the transcriptional regulation of plant fatty acid biosynthesis. In this study, GmWRI1a was overexpressed in...
Alpha-synuclein (αsyn) misfolding and aggregation underlie several neurodegenerative disorders, including Parkinson’s disease. Early oligomeric intermediates are particularly toxic yet remain challenging to detect and characterize within...
The control of gene expression by cis-regulatory DNA sequences is a conserved genomic feature. The maize booster1 gene (b1) is a naturally occurring locus that serves as a mechanistic model for the control of gene expression from a distal cis element...
For quantitative microRNA analyses in formalin-fixed paraffin-embedded (FFPE) tissue, expression levels have to be normalized to endogenous controls. To investigate the most stably-expressed microRNAs in breast cancer and its surrounding tissue, we u...
Pigs are important animals for meat production but can carry several zoonotic diseases, including the Japanese encephalitis virus, Nipah virus, and influenza viruses. Several Orthomyxoviridae and Coronavirinae respiratory viruses require cleavage of...
Transcriptome–wide association studies (TWAS) have identified several genes that are associated with qualitative traits. In this work, we performed TWAS using quantitative traits and predicted gene expressions in six brain subcortical structures in 2...
Mesenchymal stem/stromal cells (MSCs) prepared from various human tissues were stably transduced with the suicide gene herpes simplex virus thymidine kinase (HSVTK) by means of retrovirus infection. HSVTK-transduced MSCs express the suicide gene and...
Composite photocatalysts are crucial for photocatalytic hydrogen evolution. In this work, ZrO2/CdSe-diethylenetriamine (ZrO2/CdSe-DETA) heterojunction nanocomposites are synthesized, and efficiently and stably catalyzed hydrogen evolution under visib...
The transition to green energy is widely discussed by economists, journalists and politicians. As often happens, the engineers are almost inaudible. Meanwhile, there are problems. If green energy is based on wind turbines, as the most important and p...
Graphene is a 2D carbon material with peculiar features such as high electrical conductivity, high thermal conductivity, mechanical stability, and a high ratio between surface and thickness. Applications are continuously growing, and the possibility...
Background/Objectives: In chronic hepatitis B infection (CHB), the hepatitis B surface antigen (HBsAg) continuously exhausts the hepatitis B surface antibody (HBsAb), which leads to the formation of immune tolerance. Accordingly, the hepatitis B viru...
The production of recombinant proteins in bioreactors requires real-time process monitoring and control to increase process efficiency and to meet the requirements for a comprehensive audit trail. The combination of optical near-infrared turbidity se...
Bovine leukemia virus (BLV), a major cause of B-cell lymphoma in cattle worldwide, has been linked to human breast cancer. Here, we performed comparative whole-transcriptome analysis of single clones of human epithelial 293T, breast cancer MCF7, and...
Cottonseed contains many bioactive molecules including plant polyphenols. Cottonseed value might be increased by providing high-value bioactive polyphenols for improving nutrition and health. However, there was a lack of molecular evidence for cotton...
Multi-epitopes of FMDV can be used to develop a novel vaccine. Determining how to screen naturally presenting epitope peptides derived from FMDV is crucial for advancing progress in this area. In this study, a transient expression plasmid named pEGFP...
Gait analysis is an important assessment tool for analyzing vital signals collected from individuals and for providing physical information of the human body, and it is emerging in a diverse range of application scenarios, such as disease diagnosis,...
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Cytochrome P450 2A13 (CYP2A13), an extrahepatic enzyme mainly expressed in the human respiratory system, has been reported to mediate the metabolism and toxicity of cigarette smoke. We previously found that nicotine inhibited 4-(methylnitrosamino)-1-...
In chemical engineering applications, the operation of condensers and evaporators can be made more efficient by exploiting the transport properties of interfacial waves excited on the interface between a hot vapor overlying a colder liquid. Linear th...
The transcription factor nuclear factor kappa B (NF-κB) is a central regulator of inflammatory and genotoxic stress responses and is involved in immune function and disease pathogenesis. Reporter assays are widely used to quantify NF-κB a...
Due to suppression jamming by jammers, the signal-to-interference-plus-noise ratio (SINR) during tracking tasks is significantly reduced, thereby decreasing the target detection probability of radar systems. This may result in the interruption of the...
In the surgical treatment of the most common fracture of the mandible, which is a fracture of the condylar base, a great choice of different plate shapes is observed. The aim of this study was to determine which shape gives the greatest fixation stif...
Vaccination with Salmonella enterica serovar Typhimurium lacking DNA adenine methyltransferase confers cross-protective immunity against multiple Salmonella serotypes. The mechanistic basis is thought to be associated with the de-repression of genes...
Turbulence structure in the wake behind a full-scale horizontal-axis wind turbine under the influence of real-time atmospheric inflow conditions has been investigated using actuator-line-model based large-eddy-simulations. Precursor atmospheric bound...
Based on a two-step polymerization method, two sulfobetaine-based zwitterionic monomers, including 3-(methacryloylamino) propyl-dimethyl-(3-sulfopropyl) ammonium hydroxide (MPDSAH) and 2-(methacryloyloxyethyl) ethyl-dimethyl-(3-sulfopropyl) ammonium...
Regular cocoa consumption has been shown to improve blood pressure (BP), insulin sensitivity, and lipid levels in patients with type 2 diabetes (T2D), using up to 100 g of chocolate or 54 g of cocoa. These effects, attributed to cocoa flavanols, woul...
Influenza viruses infect millions of people each year, resulting in significant morbidity and mortality in the human population. Therefore, generation of a universal influenza virus vaccine is an urgent need and would greatly benefit public health. R...
To investigate the transcriptional regulation of gene expression, chimeric fusions, between the beta-glucuronidase reporter gene (GUS) and the isolated promoter regions of the pvPDF gene (pvPDF-PRO: GUS), were constructed and introduced into Nicotian...
We study a class of nonlinear operators that can be written as the composition of a linear operator and a nonlinear map. We obtain results on fixed point index based on parameters that are related to the definitions of nonlinear spectra. As a particu...
Silica spicules provide a natural transdermal conduit but require a linker that binds strongly under physiological conditions and releases payloads selectively in response to biological cues. Existing silane chemistries or polydopamine coatings lack...
MicroRNAs (miRNAs) regulate approximately one-third of all human genes. The dysregulation of miRNAs has been implicated in the development of numerous human diseases, including cancers. In our investigation focusing on altering specific miRNA express...
In an overall approach aiming at the development and qualification of various tools designed to diagnose and/or forecast the flows at the local scale in complex terrain, we qualified a numerical model based on the WRF platform and operated in a two-w...
A hydrophilic, hydrostable porous metal organic framework (MOF) material-MIL-101 (Cr) was successfully doped into the dense selective polyamide (PA) layer on the polysulfone (PS) ultrafiltration (UF) support to prepare a new thin film nanocomposite (...
Insecticide resistance threatens effective pest management. In this study on Rhopalosiphum padi, a predominant wheat pest, a field-derived beta-cypermethrin-resistant strain (Beta-R strain) showed a 4623.50-fold resistance increase after 22 generatio...
HBx plays a significant role in the cccDNA epigenetic modification regulating the hepatitis B virus (HBV) life cycle and in hepatocyte proliferation and carcinogenesis. By using the sleeping-beauty transposon system, we constructed a tetracycline-ind...
Persistent and efficient therapeutic protein expression in the specific target cell is a significant concern in gene therapy. The controllable integration site, suitable promoter, and proper codon usage influence the effectiveness of the therapeutic...
This study designed three sgRNAs (sgRNA139, sgRNA128, and sgRNA109) targeting the prolactin gene receptor (PRLR) in fetal cattle, utilized Cas9 to cleave endogenous DNA, and screened stable cell lines for somatic cell nuclear transfer experiments to...
Neovascular age-related macular degeneration (nvAMD) is characterized by choroidal neovascularization (CNV), which leads to retinal pigment epithelial (RPE) cell and photoreceptor degeneration and blindness if untreated. Since blood vessel growth is...
NiFe-layered double hydroxides (NiFe-LDH) have been reported to possess exceptional oxygen evolution reaction (OER) activity. However, maintaining the stability of high activity over a long time remains a critical challenge that needs to be addressed...
Pictilisib (GDC-0941) is a well-known dual inhibitor of class I PI3K and mTOR and is presently undergoing phase 2 clinical trials for cancer treatment. The present work investigated the dynamic behaviors and interaction mechanism between GDC-0941 and...
The hepatitis E virus (HEV) is transmitted via the faecal–oral route in developing countries (genotypes 1 and 2) or through contaminated food and blood products worldwide (genotypes 3 and 4). In Europe, HEV subtypes 3c, 3e and 3f are predominan...
In the original publication [...]
A critical aspect of real-time PCR is the presence of housekeeping genes (HKGs) as an internal control for the normalization of expression data for genes of interest. It is necessary to select correct HKGs in the investigation of various pathologies....
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