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Article

Suicide Gene Therapy Mediated with Exosomes Produced by Mesenchymal Stem/Stromal Cells Stably Transduced with HSV Thymidine Kinase

1
Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, 811 08 Bratislava, Slovakia
2
Stem Cell Preparation Laboratory, St. Elisabeth Cancer Institute, Heydukova 10, 812 50 Bratislava, Slovakia
3
Biomedical Center, Cancer Research Institute, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(5), 1096; https://doi.org/10.3390/cancers12051096
Received: 17 March 2020 / Revised: 8 April 2020 / Accepted: 24 April 2020 / Published: 28 April 2020
(This article belongs to the Special Issue The Role of Aptamers in Cancer Diagnostics and Therapy)
Mesenchymal stem/stromal cells (MSCs) prepared from various human tissues were stably transduced with the suicide gene herpes simplex virus thymidine kinase (HSVTK) by means of retrovirus infection. HSVTK-transduced MSCs express the suicide gene and in prodrug ganciclovir (GCV) presence induced cell death by intracellular conversion of GCV to GCV-triphosphate. The homogenous population of HSVTK-MSCs were found to release exosomes having mRNA of the suicide gene in their cargo. The exosomes were easily internalized by the tumor cells and the presence of ganciclovir caused their death in a dose-dependent manner. Efficient tumor cell killing of glioma cell lines and primary human glioblastoma cells mediated by HSVTK-MSC exosomes is reported. Exosomes produced by suicide gene transduced MSCs represent a new class of highly selective tumor cell targeted drug acting intracellular with curative potential. View Full-Text
Keywords: HSVTK; GCV; MSCs; suicide gene HSVTK exosomes HSVTK; GCV; MSCs; suicide gene HSVTK exosomes
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MDPI and ACS Style

Pastorakova, A.; Jakubechova, J.; Altanerova, U.; Altaner, C. Suicide Gene Therapy Mediated with Exosomes Produced by Mesenchymal Stem/Stromal Cells Stably Transduced with HSV Thymidine Kinase. Cancers 2020, 12, 1096. https://doi.org/10.3390/cancers12051096

AMA Style

Pastorakova A, Jakubechova J, Altanerova U, Altaner C. Suicide Gene Therapy Mediated with Exosomes Produced by Mesenchymal Stem/Stromal Cells Stably Transduced with HSV Thymidine Kinase. Cancers. 2020; 12(5):1096. https://doi.org/10.3390/cancers12051096

Chicago/Turabian Style

Pastorakova, Andrea, Jana Jakubechova, Ursula Altanerova, and Cestmir Altaner. 2020. "Suicide Gene Therapy Mediated with Exosomes Produced by Mesenchymal Stem/Stromal Cells Stably Transduced with HSV Thymidine Kinase" Cancers 12, no. 5: 1096. https://doi.org/10.3390/cancers12051096

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