Isolation of Subtype 3c, 3e and 3f-Like Hepatitis E Virus Strains Stably Replicating to High Viral Loads in an Optimized Cell Culture System

The hepatitis E virus (HEV) is transmitted via the faecal–oral route in developing countries (genotypes 1 and 2) or through contaminated food and blood products worldwide (genotypes 3 and 4). In Europe, HEV subtypes 3c, 3e and 3f are predominant. HEV is the leading cause of acute hepatitis globally and immunocompromised patients are particularly at risk. Because of a lack of cell culture systems efficiently propagating wild-type viruses, research on HEV is mostly based on cell culture-adapted isolates carrying uncommon insertions in the hypervariable region (HVR). While optimizing the cell culture system using the cell culture-adapted HEV strain 47832c, we isolated three wild-type strains derived from clinical specimens representing the predominant spectrum of HEV in Europe. The novel isolates 14-16753 (3c), 14-22707 (3e) and 15-22016 (3f-like) replicate to high viral loads of 108, 109 and 106.5 HEV RNA copies/mL at 14 days post-inoculation, respectively. In addition, they could be kept as persistently infected cell cultures with constant high viral loads (~109 copies/mL) for more than a year. In contrast to the latest isolates 47832c, LBPR-0379 and Kernow-C1, the new isolates do not carry genome insertions in the HVR. Optimization of HEV cell culture identified amphotericin B, distinct salts and fetal calf serum (FCS) as important medium supplements. Overconfluent cell layers increased infectivity and virus production. PLC/PRF/5, HuH-7-Lunet BLR, A549 and HepG2/C3A supported replication with different efficiencies. The novel strains and optimized cell culture system may be useful for studies on the HEV life cycle, inactivation, specific drug and vaccine development.


Introduction
The hepatitis E virus (HEV) is a small, (+) single-stranded RNA virus belonging to the family Hepeviridae, genus Orthohepevirus, which is comprised of four species. Orthohepevirus B is found in chickens and Orthohepevirus D in bats [1]. Orthohepevirus C mainly circulates in rats but has zoonotic potential [2]. HEV of Orthohepevirus A is currently classified into seven genotypes (gt) [1,3] of which 1-4 [1] and 7 [4] infect humans. Genotypes 1 and 2 are restricted to humans and are transmitted via the faecal-oral route in developing countries. Genotypes 3 and 4 are also found in pig, wild boar and deer (amongst others) and are transmitted through contaminated food and blood products worldwide [5]. Genotype 7 is linked to consumption of contaminated camelid meat and milk [4]. Moreover, a putative genotype 8 was discovered which infects Bactrian camels [6]. The 7.2 kb long genome comprises three major open reading frames (ORF). ORF1 encodes the non-structural polyprotein and ORF2 for • BMEM: Eagle minimum essential medium (MEM) supplemented with 10% heat-inactivated fetal calf serum (FCS), 2 mM l-glutamine, 1% non-essential amino acids (NEAA), 100 U/mL penicillin G and 100 µg/mL streptomycin.

Virus Isolation and Passaging
Unless stated differently in the results section, isolation was carried out in T12.5 flasks as follows: BMEM cultured cells were seeded at a concentration of 10 5 viable cells/cm 2 in T12.5 flasks 14 days prior to inoculation and cultured at 37 • C and 5% CO 2 . Medium was switched from BMEM to MEMM and completely refreshed every 3-4 days. After 14 days, supernatant was replaced with 250 µL of inoculum and cells were incubated for 75 min at room temperature. Afterwards, 2.5 mL of MEMM were added and cells were incubated at 34.5 • C and 5% CO 2 . At 24 h later, supernatant was completely refreshed with MEMM and from then on every 3-4 days.

HEV RNA Quantification
RNA was isolated on an EZ1 ® Advanced XL workstation using the EZ1 Virus Mini Kit v2.0 (Qiagen, Hilden, Germany). Eluted nucleic acid was tested by RT-qPCR according to Wenzel et al. [31]. HEV RNA was quantified as genome copies per mL (c/mL).

Detection of ORF2 Antigen
The commercially available HEV Ag ELISA Plus kit (Wantai, Beijing, China) was used for detecting ORF2 antigen. The ELISA was performed according to the manufacturer's protocol.

Whole Genome Sequencing
Isolated RNA of strain 14-16753, 14-22707 and 15-22016 was reverse transcribed by MuLV (Applied Biosystems, Waltham, MA, USA) and amplified by a first round PCR in different overlapping parts (Tables S1-S3). The cDNA synthesis of the 5 -end was carried out with the SuperScript™ III First-Strand Synthesis System kit (Thermo Fisher Scientific). Primers used for cDNA synthesis of the 5 -and 3 -end followed by a first round PCR are based on the BD SMART™ RACE cDNA Amplification kit protocol (Table S4). All other primers are either based on Johne et al. [23] or were designed using Primer3 (http://primer3.ut.ee/) [33] and GEMI v1.5.1 [34]. PCR products were further amplified by a nested PCR. These products were separated on agarose gels and all amplification products were extracted using the QIAquick Gel Extraction kit (Qiagen). Purified amplicons were then sequenced on an ABI 3130xl automated sequencer. Electropherograms were inspected and sequences assembled with CodonCode Aligner v4.2.7 (www.codoncode.com, CodonCode Corporation, Centerville, MA, USA). All PCR reactions were performed with a Veriti™ 60-well Thermal Cycler (Thermo Fisher Scientific). Consensus sequences were deposited in the GenBank under accession numbers MK089849 (14-16753, gt 3c), MK089848 (14-22707, gt 3e) and MK089847 (15-22016, gt 3f-like).

Phylogenetic Analysis
Obtained whole genome sequences were genotyped by using the current HEV subtype reference set proposed by Smith et al. [3]. Multiple sequence alignments (msa) were carried out by using MUSCLE v3.8.31 [35]. Msa-files were further processed with RAxML v8.2.10 [36] and the best matching phylogenetic tree was calculated based on the maximum likelihood principle with a bootstrap of 1000 replicates. The tree was rooted using moose HEV [37] as an outgroup and visualized by FigTree v1.4.3 (http://tree.bio.ed.ac.uk/software/figtree/).

Effect of Medium Refreshment on Cells Infected With HEV Strain 47832c and Isolation of HEV Gt 3c Strain 14-16753
Supernatant of A549 persistently infected with genotype (gt) 3c strain 47832c was examined after different time points. A maximum of HEV RNA accumulated after 64 h whereas HEV ORF2 antigen (Ag) concentrations kept on rising even if medium was not refreshed until 168 h ( Figure 1a). These data indicate that there is no need for daily medium refreshment to reach high viral loads.
To analyse the effect of medium exchange frequency, supernatants were either half or completely refreshed in different intervals. After five weeks, higher HEV RNA concentrations were generated when medium was refreshed completely and more frequently (Figure 1b). However, refreshing medium once or twice a week still resulted in high HEV RNA concentrations.
The impact of medium refreshment on de novo infection of cell lines was also investigated by inoculating A549 and PLC/PRF/5 with isolate 47832c (A549 supernatant containing 2.1 × 10 7 c/mL). In addition, HEV-positive material of three different subjects were inoculated: a faecal suspension 14-16078 (1.1 × 10 4 c/mL, gt 3e), serum 14-16753 (2.8 × 10 6 c/mL, gt 3c) and plasma 13-14672 (4.3 × 10 3 c/mL, gt 3 subtype not assignable). For strain 47832c, no difference was detected between medium refreshment once, twice or five times a week. An exception was weekly refreshment of A549 resulting in lower HEV ORF2 Ag concentrations (Figure 1c,d). Cells inoculated with human specimens showed low antigen concentrations. However some elevated values were detected for the serum sample and A549 inoculated with the faecal suspensions and refreshed five times a week. After 7 weeks HEV RNA was only detectable in the supernatant of A549 and PLC/PRF/5 inoculated with serum (long-term cultivation shown in Figure 6a,b). This experiment represented the first successful isolation of the novel HEV gt 3c strain labelled 14-16753.
Viruses 2019, 11,483 5 of 20 refreshment once, twice or five times a week. An exception was weekly refreshment of A549 resulting in lower HEV ORF2 Ag concentrations (Figure 1c&d). Cells inoculated with human specimens showed low antigen concentrations. However some elevated values were detected for the serum sample and A549 inoculated with the faecal suspensions and refreshed five times a week. After 7 weeks HEV RNA was only detectable in the supernatant of A549 and PLC/PRF/5 inoculated with serum (long-term cultivation shown in Figure 6a&b). This experiment represented the first successful isolation of the novel HEV gt 3c strain labelled 14-16753. Taken together, complete medium refreshment once a week is appropriate for HEV-positive PLC/PRF/5 cells but not for A549. Therefore, complete medium refreshment twice a week was applied in the following experiments. Supernatant of A549 persistently infected with HEV strain 47832c was either changed half or completely once, twice or five times a week and tested weekly for HEV RNA. (c) A549 and (d) PLC/PRF/5 were inoculated in technical duplicates with isolate 47832c as well as with HEV-positive materials from three different patients, namely a faecal suspension 14-16078, serum 14-16753 and plasma 13-14672. After inoculation, medium was changed completely once, twice or five times a week and supernatants were tested for HEV ORF2 Ag 24 days post-inoculation (dpi). SCR, signal-to-cut-off ratio. After inoculation, medium was changed completely once, twice or five times a week and supernatants were tested for HEV ORF2 Ag 24 days post-inoculation (dpi). SCR, signal-to-cut-off ratio.

Overconfluently Grown Cells
Taken together, complete medium refreshment once a week is appropriate for HEV-positive PLC/PRF/5 cells but not for A549. Therefore, complete medium refreshment twice a week was applied in the following experiments.

Overconfluently Grown Cells Seeded >1 Week Prior to Inoculation are More Permissive for Infection
Since an HEV strain derived from an experimentally infected pig exclusively replicated in three-dimensional cultures of PLC/PRF/5 [19], the effect of cell confluence on susceptibility to HEV was investigated. A549 and PLC/PRF/5 were seeded at different concentrations and time points prior to inoculation with strain 47832c (A549 supernatant containing 2.6 × 10 8 c/mL). At 0 days prior to cell seeding, 2.5 mL of defined cell suspensions were transferred to T12.5 flasks and 250 µl of inoculum was immediately added before cells could adhere. Supernatants were tested for HEV RNA after 14 and 28 days post inoculation (dpi). Detection of HEV RNA at 28 dpi represented a successful infection. All A549 cultures were successfully infected with HEV ( Figure 2a). Cells seeded at least 7-10 days (depending on cell concentration) prior to virus inoculation generated higher viral loads in shorter periods. This trend was clearly more pronounced in PLC/PRF/5 (Figure 2b), which could only be reproducibly infected when seeding cells at the default split ratio of 1:4 at least 14 days before inoculation. This lead time can be shortened to 10 and 7 days by seeding 1 × 10 6 to 9 × 10 6 viable cells, respectively. The fact, that viral load at 28 dpi does not exceed the inoculum's load (except for PLC/PRF/5 seeded 35 days prior to inoculation) may be due to the slow replicating nature of HEV. Since an HEV strain derived from an experimentally infected pig exclusively replicated in threedimensional cultures of PLC/PRF/5 [19], the effect of cell confluence on susceptibility to HEV was investigated. A549 and PLC/PRF/5 were seeded at different concentrations and time points prior to inoculation with strain 47832c (A549 supernatant containing 2.6 × 10 8 c/mL). At 0 days prior to cell seeding, 2.5 mL of defined cell suspensions were transferred to T12.5 flasks and 250 µl of inoculum was immediately added before cells could adhere. Supernatants were tested for HEV RNA after 14 and 28 days post inoculation (dpi). Detection of HEV RNA at 28 dpi represented a successful infection. All A549 cultures were successfully infected with HEV ( Figure 2a). Cells seeded at least 7-10 days (depending on cell concentration) prior to virus inoculation generated higher viral loads in shorter periods. This trend was clearly more pronounced in PLC/PRF/5 (Figure 2b), which could only be reproducibly infected when seeding cells at the default split ratio of 1:4 at least 14 days before inoculation. This lead time can be shortened to 10 and 7 days by seeding 1 × 10 6 to 9 × 10 6 viable cells, respectively. The fact, that viral load at 28 dpi does not exceed the inoculum's load (except for PLC/PRF/5 seeded 35 days prior to inoculation) may be due to the slow replicating nature of HEV.
Generally, we observed a tendency to more reliable infection and higher viral loads when more cells are seeded earlier prior to inoculation, generating more susceptible three-dimensional cell  Generally, we observed a tendency to more reliable infection and higher viral loads when more cells are seeded earlier prior to inoculation, generating more susceptible three-dimensional cell layers. Therefore and to standardize the approach, cell seeding was set to 1 × 10 5 viable cells per cm 2 at 14 days prior to inoculation.

Distinct Medium Supplements Enhance HEV Replication and Optimized Media Promote De Novo Isolation of HEV Gt 3e Strain 14-22707
The concentration of supplemented FCS is usually reduced in medium used for virus culturing. Therefore, A549 persistently infected with isolate 47832c were maintained in BMEM_G containing different concentrations of heat-inactivated FCS. The supernatant was completely refreshed and tested for HEV ORF2 Ag after different periods. Incubating HEV-positive cells with higher concentrations of FCS resulted in higher HEV ORF2 Ag production (Figure 3a).   Previous HEV isolation trials used medium supplements amphotericin B and 30 mM MgCl2 [14] or non-essential amino acids (NEAA) [23] and different antibiotics. In addition, a serial dilution experiment revealed supplementation of 10 mM CaCl 2 , KCl, K 2 SO 4 , MgCl 2 , MgSO 4 or Na 2 SO 4 to enrich HEV ORF2 Ag concentrations in the supernatant whereas KH 2 PO 4 , NaCl and Na 2 HPO 4 decreased antigen concentrations (data not shown). Therefore, A549 and PLC/PRF/5 maintained in differently supplemented BMEM_G were inoculated with isolate 47832c-containing A549 supernatant (2.6 × 10 8 c/mL). Subsequent supernatants were tested for HEV RNA after 14 dpi and ORF2 Ag after 28 dpi. Medium supplements enhancing HEV replication most distinctly are shown in Figure 3b. Surprisingly, supplementing 2.5 µg/mL amphotericin B resulted in the highest increase of HEV RNA and ORF2 Ag, at least in PLC/PRF/5 supernatant. Supplementation of salts revealed K 2 SO 4 combined with CaCl 2 to enhance HEV replication most pronouncedly. However, cell viability decreased by~50% in presence of supplemented CaCl 2 . Viral loads were also increased in PLC/PRF/5 but not in A549 by supplementing NEAA. Adding 100 U/mL penicillin G and 100 µg/mL streptomycin resulted in slightly higher HEV RNA and ORF2 Ag levels compared to 100 µg/mL gentamycin (data not shown).
Based on these results, A549, PLC/PRF/5, HepG2/C3A and HuH-7-Lunet BLR either maintained with BMEM, MECK (BMEM supplemented with 2.5 µg/mL amphotericin B, 10 mM CaCl 2 and 10 mM K 2 SO 4 ) or MEMM (adapted from Tanaka et al. [14]; BMEM supplemented with 2.5 µg/mL amphotericin B and 30 mM MgCl 2 ) were inoculated with PLC/PRF/5 supernatant containing isolate 47832c (5.7 × 10 6 c/mL), isolate 14-16753 (8.3 × 10 6 c/mL) or with an HEV gt 3e-positive serum (1.6 × 10 6 c/mL). At 49 dpi, higher viral loads were always found in the supernatant of cells cultivated with MECK or MEMM compared to BMEM (Figure 3c). HEV gt 3e was successfully isolated from serum in PLC/PRF/5 and A549 irrespective of the medium. In contrast, HEV gt 3e could only be isolated in HuH-7-Lunet BLR maintained in optimized medium and HepG2/C3A was not susceptible to HEV gt 3e at all. The novel isolate was labelled 14-22707 (long-term cultivation shown in Figure 6c). MEMM showed better results than MECK in three out of four cell lines and was therefore selected as the culture medium of choice. Many different cell lines have already been tested for their capability of supporting the replication of HEV in vitro. A549, its subclone A549/D3, PLC/PRF/5 and HepG2/C3A were found to be the most susceptible cell lines to HEV [14,21,22,30]. However, these cell lines were never systematically compared with different HEV strains. In addition, the cell clone HuH-7-Lunet BLR might also be a potential candidate for HEV since it is highly permissive to hepatitis C virus replication [29].
Therefore, PLC/PRF/5 as a three-dimensional cell layer is the most suitable host for isolating and maintaining HEV in vitro. The newly introduced cell clone HuH-7-Lunet BLR also clearly supports the replication of HEV and generates higher viral loads than A549 and HepG2/C3A.

Long-Term Cultivation of Persistently Infected Cells Leads to Virtually Unlimited Production of HEV
The novel isolated HEV strains together with isolate 47832c were followed up for a period of 30 weeks to more than 2 years, whereby no cytopathic effect was ever observed. The highest viral loads detected were 7.4 × 10 8 c/mL (208 dpi) and 1.5 × 10 9 c/mL (476 dpi) for isolation and first passage of strain 14-16753, respectively (Figure 6a,b). The maximum of isolate 14-22707 and 15-22016 was detected at 134 dpi with 4.4 × 10 9 c/mL and at 274 dpi with 5.5 × 10 9 c/mL, respectively (Figure 6c,d).
Remarkably, cells could be maintained for more than a year in one single T12.5 flask by solely refreshing the medium completely every 3-4 days. Over the whole period, infectious HEV was secreted into the medium resulting in high viral loads. All isolates were successfully passaged. Moreover, HEV positive cells were successfully expanded, frozen, thawed and re-cultured again and continued to produce infectious HEV. Surprisingly, the viral load of the first passage of isolate 14-16753 constantly decreased when cells were split regularly (Figure 6b). In contrast, this was not observed with isolate 14-22707 ( Figure 6c) and will be investigated further in future experiments.

Optimized Method for Successful HEV Isolation from Serum
According to the results of the study, an optimized protocol for HEV isolation from serum in cell culture should include: Seeding PLC/PRF/5 in T12.5 flasks at a concentration of 10 5 viable cells per cm 2 . Maintaining the cells in MEMM at 37 • C and 5% CO 2 for two weeks and refreshment of medium every 3-4 days. If necessary, diluting HEV positive serum to an adequate volume with PBS without Ca 2+ and Mg 2+ containing 0.2% BSA (w/v). Vortexing, centrifuging at 8000× g for 10 min and sterile-filtering using a 0.2 µm PES membrane. No further pretreatment is needed. Afterwards, the inoculum should at least contain 10 5 HEV RNA copies/mL. The three-dimensional cell layer was then inoculated with 250 µL per T12.5 for 75 min at room temperature. 2.5 mL MEMM was then added and incubated at 34.5 • C and 5% CO 2 . The medium was completely refreshed 24 h later and every 3-4 days afterwards.

Discussion
Several approaches to HEV cell culture have already been described. However, these approaches could either not fully be reproduced [18][19][20], could only isolate strains carrying an uncommon insertion in ORF1 [21][22][23] or need a sophisticated setup to generate three-dimensional cultures [19]. This work aimed to improve the published approaches to HEV cell culture, make it an easy-care system which can be continuously maintained for virtually unlimited periods of time and isolate novel wild-type HEV strains.
Optimization experiments led to the isolation of three novel strains labelled 14-16753 (genotype (gt) 3c), 14-22707 (gt 3e) and 15-22016 (gt 3f-like). These isolates replicate to high viral loads of >10 9 c/mL and differ in replication kinetics. Moreover, this is the first time that whole genome sequences of two stably cell culture propagated isolates of clade 3efg were determined. In addition, the three new isolates represent the predominant subtypes 3c, 3e and 3f currently circulating in Europe.
Our results show that (i) maintenance effort can be decreased substantially by reducing medium refreshment from daily to twice a week, (ii) overconfluent (three-dimensional) cell layers grown in flasks are more susceptible to HEV compared to usual monolayers, (iii) medium supplements, especially amphotericin B, MgCl 2 , CaCl 2 and K 2 SO 4 increase HEV replication in vitro, (iv) PLC/PRF/5 as a three-dimensional cell layer is the most permissive cell line to HEV and HuH-7-Lunet BLR also substantially supports HEV replication, (v) HEV-producing cells can be kept in culture for >1 year with only medium refreshment every 3-4 days and still generate very high viral loads of~10 9 c/mL. Surprisingly, the susceptibility of overconfluently grown cells to HEV infection depends on the cell line. For instance, A549 can be seeded at virtually any time at almost any concentration and still support HEV replication. However, higher viral loads are achieved faster with mature A549 cell layers. This effect is more pronounced in PLC/PRF/5 which must be seeded ≥14 days prior to inoculation at their default split ratio to guarantee HEV infection. The lead time of 14 days can be reduced to as low as 0 if the concentration of seeded cells is increased. For a reliable infection, however, 7 days of cell growing are still needed to generate a susceptible overconfluent cell layer forming three-dimensional structures. This stands in line with Berto et al. who observed that solely differentiated PLC/PRF/5 grown as three-dimensional cultures support HEV replication but not common monolayers [19]. Cell differentiation and closer contacts between cells in the three-dimensional cell layers may result in a higher susceptibility to HEV. In addition, autophagy processes may be considered since overconfluent cell layers consisting of much more cells compared to monolayers, were maintained in the same amount of medium and may therefore run low on nutrients.
Medium supplements are also important for HEV isolation and maintenance. In 1999, Huang et al. reported that 30 mM MgCl 2 supplemented to the maintenance medium increased HEV loads and preserved infectivity [41]. We found that not only MgCl 2 but also 10 mM CaCl 2 , KCl, K 2 SO 4 , MgSO 4 and Na 2 SO 4 benefit to HEV replication whereas KH 2 PO 4 , NaCl and Na 2 HPO 4 adversely affect the replication. The FCS concentration also influences the potential of HEV replication in vitro. Commonly, 2% FCS are added to a virus maintaining medium. However, for HEV, we found that 10% FCS was more appropriate. The medium supplement with the highest impact on viral loads was found to be amphotericin B. Noteworthy, amphotericin B also promotes influenza virus replication in cell culture [42]. The authors hypothesized that the antimycotic drug contributes to the acidification of internal cell compartments which in turn promote fast pH decrease within endosomes and therefore virus infectivity. Since HEV enters liver cells via clathrin-mediated endocytosis [43] and HEV infectivity depends on acidification of endosomes [44] these facts would be in line with the observed influence of amphotericin B on HEV replication in vitro.
HEV was described to replicate in several cell lines and A549, PLC/PRF/5 and HepG2/C3A were the most promising ones [14,21]. Therefore, these cell lines were investigated for HEV replication competence together with cell clones A549/D3 (supports HEV strain 47832c replication more efficiently [30]) and HuH-7-Lunet BLR (highly permissive for hepatitis C virus replication [29]). Until 14 dpi it is strain-dependent which cell line generates the highest viral loads. But as of week four post inoculation PLC/PRF/5 always generate the highest viral loads independent of isolation or passaging of a strain. High viral loads were also generated with HuH-7-Lunet BLR which were generally higher compared to A549 and HepG2/C3A.
The latest propagated HEV strains 47832 [23], LBPR-0379 [22] and Kernow-C1 [21] were isolated from chronically infected patients and harbour an insertion in the ORF1 either acquired by recombination [21,22] or derived from its own ORF1 [23]. HEV genomes of Kernow-C1 harbouring an insertion in ORF1 were classified as a minor species [21] and became the predominant species in cell culture [22]. Moreover, the insertion confers a growth advantage in vitro [40]. Our isolates do not harbour insertions in ORF1. This indicates that our optimized cell culture system is not only permissive to adapted minor species but also to wild-type dominant species.
Our isolates have been kept in culture continuously for 1 to >2.5 years and persistently infected cells still produced viral loads of~10 9 c/mL. Presumably, HEV-positive cells can be continuously maintained for a virtually unlimited period of time. This is an important aspect since immunocompromised patients chronically infected with HEV rely on an effective antiviral treatment. However, only off-label drugs such as ribavirin and sofosbuvir are currently available. While treatment with ribavirin leads to a sustained virologic response in three-quarters of patients [45] there are already numerous ribavirin-associated mutations described [46] which lead to treatment failure [47]. Sofosbuvir was shown to inhibit HEV Kernow-C1 replication in vitro and the combination with ribavirin even resulted in an additive effect [48]. However, there is not enough clinical data yet to estimate the effectivity in vivo due to few contradictory reports [49][50][51]. Recent reports suggest silvestrol as a drug candidate to treat HEV [52,53]. Our long-term HEV producing cell culture model may step in here and serve as a more lifelike setting to test drug candidates in vitro.
There are several uncertainties and possible limitations. First, HEV replicates very slowly to adequate viral concentrations after 1-10 weeks. This may hinder fast application of down-stream processes. Second, due to the absence of a cytopathic effect, replication has to be specifically confirmed by consecutive PCR testing or viral antigen detection. Third, it needs to be examined, if HEV of genotypes other than gt 3 can be propagated with comparable efficiency in the optimized cell culture system. Fourth, our cell culture system is based on a continuous cell line that may not reflect cells in vivo. Fifth, the question arises if and how the genome of the isolated strains changes after long-term cultivation or repeated passaging. Finally, our cell culture system does not include animal-derived cells which may be more useful for studying zoonotic aspects of HEV.
In summary, we isolated three novel HEV strains of the predominant subtypes in Europe stably replicating to high viral loads while optimizing and simplifying the cell culture system. This system may be useful for studies on the HEV life cycle, inactivation, drug and vaccine development.