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Transcriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments

1
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
2
Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA
3
Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, VA 24061, USA
*
Authors to whom correspondence should be addressed.
Pathogens 2014, 3(2), 417-436; https://doi.org/10.3390/pathogens3020417
Received: 1 March 2014 / Revised: 30 April 2014 / Accepted: 4 May 2014 / Published: 9 May 2014
(This article belongs to the Special Issue Bacterial Pathogenomics: From Technology to Application)
Vaccination with Salmonella enterica serovar Typhimurium lacking DNA adenine methyltransferase confers cross-protective immunity against multiple Salmonella serotypes. The mechanistic basis is thought to be associated with the de-repression of genes that are tightly regulated when transiting from one microenvironment to another. This de-repression provides a potential means for the production of a more highly expressed and stable antigenic repertoire capable of inducing cross-protective immune responses. To identify genes encoding proteins that may contribute to cross-protective immunity, we used a Salmonella Typhimurium DNA adenine methyltransferase mutant strain (UK-1 dam mutant) derived from the parental UK-1 strain, and assessed the transcriptional profile of the UK-1 dam mutant and UK-1 strain grown under conditions that simulate the intestinal or endosomal microenvironments encountered during the infective process. As expected, the transcriptional profile of the UK-1 dam mutant identified a set of genes more transcriptionally active when compared directly to UK-1, and stably transcribed in biologically relevant culture conditions. Further, 22% of these genes were more highly transcribed in comparison to two other clinically-relevant Salmonella serovars. The strategy employed here helps to identify potentially conserved proteins produced by the UK-1 dam mutant that stimulate and/or modulate the development of cross-protective immune responses toward multiple Salmonella serotypes. View Full-Text
Keywords: Salmonella Typhimurium; DNA adenine methyltransferase; transcriptome; immunity; bacteriophage; fimbriae Salmonella Typhimurium; DNA adenine methyltransferase; transcriptome; immunity; bacteriophage; fimbriae
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MDPI and ACS Style

Miller, C.B.; Pierlé, S.A.; Brayton, K.A.; Ochoa, J.N.; Shah, D.H.; Lahmers, K.K. Transcriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments. Pathogens 2014, 3, 417-436. https://doi.org/10.3390/pathogens3020417

AMA Style

Miller CB, Pierlé SA, Brayton KA, Ochoa JN, Shah DH, Lahmers KK. Transcriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments. Pathogens. 2014; 3(2):417-436. https://doi.org/10.3390/pathogens3020417

Chicago/Turabian Style

Miller, Claire B.; Pierlé, Sebastian A.; Brayton, Kelly A.; Ochoa, Jennine N.; Shah, Devendra H.; Lahmers, Kevin K. 2014. "Transcriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments" Pathogens 3, no. 2: 417-436. https://doi.org/10.3390/pathogens3020417

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