Three-dimensional (3D) printing technology holds great potential to fabricate complex constructs in the field of regenerative medicine. Researchers in the surgical fields have used 3D printing techniques and their associated biomaterials for educatio...
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological...
As micron-sized objects, mobile microrobots have shown significant potential for future biomedical applications, such as targeted drug delivery and minimally invasive surgery. However, to make these microrobots viable for clinical applications, sever...
Three-dimensional fluorescence is currently studied by methods such as parallel factor analysis (PARAFAC), fluorescence regional integration (FRI), and principal component analysis (PCA). There are also many studies combining convolutional neural net...
The use of 3D in vitro tumor models has become a common trend in cancer biology studies as well as drug screening and preclinical testing of drug candidates. The transition from 2D to 3D matrix-based cell cultures requires modification of methods for...
The aim of this work is to present a new practical approach to digital photogrammetry to obtain 3D models of polychromatic sculptures under ultraviolet fluorescence and near-infrared by starting from photographic images. This digital photogrammetry w...
As the primary peripheral relay station for vibrissal tactile information, the trigeminal ganglion (TG) features heterogeneous three-dimensional (3D) cytoarchitecture that eludes full characterization using conventional two-dimensional methodologies....
High-sensitivity fluorescence monitoring has been widely used in agriculture and environmental science. However, the active fluorescence detection information of leaf segments mainly focuses on total chlorophyll, and the fluorescence information of c...
Two new hetero-trinuclear 3d-4f complexes [Zn2(L)Ho(μ2-OAc)2(OAc)(MeOH)]·CH2Cl2 (1) and [Zn2(L)Er(μ2-OAc)2]OAc (2), derived from a bis(salamo)-based ligand H4L, were synthesized and characterized via elemental analyses, IR, UV–Vis, fluorescence spect...
We report the study of seven commercially available rosehip oils (Rosa canina L.) using GC-MS, colorimetry (CIELab), UV-VIS, FTIR, and 3D EEM fluorescence spectroscopy, including using a smartphone spectrometer. GC-MS revealed two groups of oil sampl...
The potential application of multivariate three-way data analysis techniques, namely parallel factor analysis (PARAFAC) and discriminant multi-way partial least squares regression (DN-PLSR), on three-dimensional excitation emission matrix (3D-EEM) fl...
N-terminal acetylation catalyzed by N-terminal acetyltransferases (NATs) has various biological functions in protein regulation. N-terminal acetyltransferase D (NatD) is one of the most specific NAT with only histone H4 and H2A proteins as the known...
Basidiomycete mushrooms contain complex β-D-glucans which act as immunomodulator, immune stimulants and anti-cancer agents, which can be either free or bound to proteins. The present report consists of a novel and intrinsic synchronous fluoresce...
Thermoplastic polyimides (TPIs) are promising lightweight materials for replacing metal components in aerospace, rocketry, and automotive industries. Key TPI attributes include low density, thermal stability, mechanical strength, inherent flame retar...
Chemical Oxygen Demand (COD) is one of the indicators of organic pollution in water bodies. The rapid and accurate detection of COD is of great significance to environmental protection. To address the problem of COD retrieval errors in the absorption...
The monitoring of a membrane bioreactor (MBR) requires the assessment of both biological and membrane performance. Additionally, the development of membrane fouling and the requirements for frequent membrane cleaning are still major concerns during M...
Fruit and coffee industries are responsible for huge quantities of agro-industrial wastes which is of great environmental and public health concern. Therefore, the aim of this work involves the use of such wastes for the production of β-D-glucan...
Single-domain antibodies (sdAbs) offer great features such as increased stability but are hampered by a limited serum half-life. Many strategies have been developed to improve the sdAb half-life, such as protein engineering and controlled release sys...
Lab-on-a-chip devices offer high efficiency, low volume and fast analytical measurement, but their use is still niche. A key component for these devices is the detector, and one common type of detection is fluorescence spectroscopy. However, in some...
The electrostatic MEMS scanner plays an important role in the miniaturization of the microscopic imaging system. We have developed a new two-dimensional (2D) parametrically-resonant MEMS scanner with patterned Au coating (>90% reflectivity at an N...
Quantum dots (QDs) are very attractive nanomaterials for analytical chemistry, due to high photostability, large surface area featuring numerous ways of bioconjugation with biomolecules, usually high quantum yield and long decay times. Their broad ab...
Multiplexed fluorescent immunohistochemical analysis of breast cancer (BC) markers and high-resolution 3D immunofluorescence imaging of the tumor and its microenvironment not only facilitate making the disease prognosis and selecting effective antica...
The catalytically inactive mutant of Cas9 (dCas9) endonuclease has multiple biomedical applications, with the most useful being the activation/repression of transcription. dCas9 family members are also emerging as potential experimental tools for gen...
The chemical reaction of 2-(methylsulfinyl)naphtho[2,3-d]thiazole-4,9-dione (3) using different amines, including benzylamine (4a), morpholine (4b), thiomorpholine (4c), piperidine (4d), and 4-methylpiperazine (4e), produced corresponding new tricycl...
In this paper, we present a sensitive and highly reproducible fluorescence immunosensor for detecting PSA in human serum. A unique feature of this study is that it uses creatively designed paddle screw-type devices and their custom-made rotating syst...
Comprehensive and accurate vegetation monitoring is required in forestry and agricultural applications. The optical remote sensing method could be a solution. However, the traditional light detection and ranging (LiDAR) scans a surface to create poin...
Genetically encoded red fluorescent proteins with a large Stokes shift (LSSRFPs) can be efficiently co-excited with common green FPs both under single- and two-photon microscopy, thus enabling dual-color imaging using a single laser. Recent progress...
The use of 2D fluorescence spectra is a powerful, instantaneous, and highly accurate method to estimate the state of bioprocesses. The conventional approach for calibrating a chemometric model from raw spectra needs a large number of offline measurem...
Light-Sheet Fluorescence Microscopy has recently emerged as the technique of choice for obtaining high quality three-dimensional (3D) images of whole organisms, with low photo-damage and fast acquisition rates. Unlike conventional optical and confoca...
Non-radiative cell membrane associated Förster Resonance Energy Transfer (FRET) from an enhanced cyan fluorescent protein (ECFP) to an enhanced yellow fluorescent protein (EYFP) is used for detection of apoptosis in 3-dimensional cell cultures. FRET...
Aggregation-induced quenching (ACQ) is a hard problem in fluorescence material, leading to a poor utilization rate of fluorophores. In this work, 1,3,6,8-tetrakis(4-formylphenyl)pyrene (TFPPy) was synthesized and used as a precursor to build two kind...
Herein, a novel fluorescent method for the determination of d-panthenol (DP) level in solutions with no separate hydrolysis step has been revealed based on the utilization of citric acid (CA) as a derivatizing agent. Consequently, the essential param...
Although it is quite challenging to image and analyze the spatial distribution of bioaerosols in a confined space, a three-dimensional (3D) modeling system based on the planar laser-induced fluorescence (PLIF) technique is proposed in this paper, whi...
Near-infrared-II (NIR-II, 1000–1700 nm) fluorescence imaging boasts high spatial resolution and deep tissue penetration due to low light scattering, reduced photon absorption, and low tissue autofluorescence. NIR-II biological imaging is applie...
Organic violet-blue fluorescent materials have garnered significant interest for a broad spectrum of applications. A series of triazine-based molecules, that is, 2,4,6-tri(9H-carbazol-9-yl)-1,3,5-triazine (TCZT), 2,4,6-tri(1H-indol-1-yl)-1,3,5-triazi...
Hollow alginate microfibers (od = 1.3 mm, id = 0.9 mm, th = 400 µm, L = 3.5 cm) comprised of 2% (w/v) medium molecular weight alginate cross-linked with 0.9 M CaCl2 were fabricated to model outward diffusion capture by 2D fluorescent microscopy. A t...
Multi-wavelength (2D) fluorescence spectroscopy represents an important step towards exploiting the monitoring potential of microtiter plates (MTPs) during early-stage bioprocess development. In combination with multivariate data analysis (MVDA), imp...
Background/Objectives: Caries adjacent to restorations remain a leading cause of restoration failure and replacement. Conventional diagnostic methods are limited by subjectivity and restricted visualization. Fluorescence-enhanced three-dimensional (3...
In this manuscript, silver nanowire 3D random crossed-wire woodpile (3D-RCW) nanostructures were designed and prepared. The 3D-RCW provides rich “antenna” and “hot spot” effects that are responsive for surface-enhanced Raman s...
3D tumor spheroids have arisen in the last years as potent tools for the in vitro screening of novel anticancer therapeutics. Nevertheless, to increase the reproducibility and predictability of the data originated from the spheroids it is still neces...
Levofloxacin (LVFX), a fluoroquinolone antibacterial agent widely used in treating bacterial infections, poses significant risks when overused, necessitating the development of reliable and efficient detection methods. Herein, we introduce Eu@SUZ&min...
Long-term changes in dissolved organic matter (DOM) quality, especially in humic-rich raw waters, may lead to intensive adaptions in drinking-water processing. However, seasonal DOM quality changes in standing waters are poorly understood. To fill th...
The development of fluorescent molecular imprinting sensors for direct, rapid, and sensitive detection of small organic molecules in aqueous systems has always presented a significant challenge in the field of detection. In this study, we successfull...
Quantitative characterisation through mineral liberation analysis is required for effective minerals processing in areas such as mineral deposits, tailings and reservoirs in industries for resources, environment and materials science. Current practic...
In this work, we developed and validated two novel imaging geometries of benchtop multi-pinhole X-ray fluorescence computed tomography (XFCT) systems with Geant4 Toolkit. One of the Monte Carlo (MC) models utilized a fan beam source to illuminate a s...
Thermochromic fluorescent materials have significant application prospects in the field of temperature sensing. In this study, based on the donor–acceptor–donor (D-A-D) strategy, two fluorescent molecules, MPT-1 and MPT-2, with fluorenyl...
A molecular imaging probe to fluorescently image the β-site of the amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) and cathepsin D (CatD) enzymes associated with Alzheimer’s disease (AD) was designed and synthesized. This imaging...
A three-dimensional quantitative structure-activity relationship (3D-QSAR) model of sulfonamide analogs binding a monoclonal antibody (MAbSMR) produced against sulfamerazine was carried out by Distance Comparison (DISCOtech), comparative molecular fi...
A novel method for the labeling and rapid separation of morphine, morphine-3-beta-d-glucuronide (M3G) and morphine-6-beta-d-glucuronide (M6G) in human urine employing a new boronic acid functionalized squarylium dye (SQ-BA3) and capillary electrophor...
The fluorescence phenomenon of apatite is an important feature. In this paper, three apatites with uniform transition from green to blue were selected, and the fluorescence color characteristics of the samples were observed under UV fluorescent lamp...
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