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Photonics
Special Issues
Micro Fluorescence Detectors/Sensors and Their Applications
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Micro Fluorescence Detectors/Sensors and Their Applications

A special issue of Photonics (ISSN 2304-6732).

Deadline for manuscript submissions: closed (20 July 2023) | Viewed by 7994

Special Issue Editors

Prof. Dr. Xuhui Geng

E-Mail Website
Guest Editor
Dalian Institute of Chemical Physics Chinese Academy of Sciences, Dalian, China
Interests: fluorescence detector; miniaturized analytical instruments, and deep sea/deep space detection
Prof. Dr. Haofeng Hu

E-Mail Website
Guest Editor
China School of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin, China
Interests: polarimetric imaging; polarimetry; deep learning; ocean optics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Fluorescence detection is one of the most sensitive detection methods, which can not only be used to study the physicochemical behavior of molecules, but also widely used in micro and trace analysis in the fields of pharmaceutical industry, environmental monitoring, food safety, etc. With the development of the semiconductor industry, the volume and power consumption of the micro fluorescence detectors/sensors that use light-emitting diode (LED) and laser diodes (LD) as the light source and photodiode (PD) and avalanche diode (APD) as the photodetector have been reduced by more than one order of magnitude compared with desktop fluorescence detectors, and the sensitivity of the micro fluorescence detectors/sensors is still comparable to that of the desktop instruments. Furthermore, micro fluorescence detectors/sensors have been freed from the limitation of size, weight, and power consumption due to the high integration and miniaturization of the instrument, thus playing an important role in the fields of micro total analysis system (lab-on-a-chip), on-site rapid analysis, and in situ analysis in extreme environments.

We are pleased to invite you to contribute to the Special Issue of “Micro fluorescence detectors/sensors and their applications”, which focuses on covering the state-of-the-art development of micro fluorescence detectors/sensors.

This Special Issue aims to collect original research articles in micro fluorescence detectors/sensors and their applications. Review articles as well as short communications are also welcome.

Research areas may include (but are not limited to) the following:

  • Novel micro fluorescence detectors;
  • Novel fluorescence sensors;
  • Miniaturization device;
  • Applications.

I look forward to receiving your contributions.

Prof. Dr. Xuhui Geng
Prof. Dr. Haofeng Hu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Photonics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • micro fluorescence detectors
  • fluorescence sensors
  • miniaturization
  • light-emitting diode (LED)
  • laser diodes (LD)
  • photodiode (PD)
  • avalanche diode (APD)
  • environmental monitoring
  • food safety

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Published Papers (5 papers)

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Research

10 pages, 2187 KiB  
Article
The Correlation between Surface V-Shaped Defects and Local Breakdown Phenomena in GaN-Based LEDs
by Seung-Hye Baek, Dae-Choul Choi, Yoon Seok Kim, Hyunseok Na and Sung-Nam Lee
Photonics 2023, 10(10), 1103; https://doi.org/10.3390/photonics10101103 - 29 Sep 2023
Viewed by 1014
Abstract
This paper investigates the intriguing impact of surface V-shaped defects on the electrical and optical characteristics of GaN-based LEDs, particularly under reverse bias conditions. These defects introduce unique luminescence phenomena, notably giant dot-like luminescence (GDL), and exert significant influence on device performance. The [...] Read more.
This paper investigates the intriguing impact of surface V-shaped defects on the electrical and optical characteristics of GaN-based LEDs, particularly under reverse bias conditions. These defects introduce unique luminescence phenomena, notably giant dot-like luminescence (GDL), and exert significant influence on device performance. The size of these V-shaped defects plays a critical role: larger defects generate more indium-rich regions at their base, resulting in elevated tensile stress. This heightened stress promotes carrier tunneling, increasing reverse leakage current and leads to GDL formation. However, even with multiple V-shaped defects present, localized failure predominantly occurs at the defect experiencing the highest tensile stress, substantially reducing the breakdown voltage. Micro-Raman analysis further reveals distinct Raman shifts and increased tensile stress in these regions. These findings underscore the complexity of V-shaped defects’ effects, highlighting their importance in GaN-based LED design and optimization. Recognizing their influence on electrical and optical properties can significantly impact device reliability and performance, particularly in reverse bias conditions. Full article
(This article belongs to the Special Issue Micro Fluorescence Detectors/Sensors and Their Applications)
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18 pages, 3712 KiB  
Article
Optical Proxies of Euxinia: Spectroscopic Studies of CDOM, Chlorophyll, and Bacteriochlorophylls in the Lagoon on Zeleny Cape (the White Sea)
by Yu. G. Sokolovskaya, E. D. Krasnova, D. A. Voronov, D. N. Matorin, A. A. Zhiltsova and S. V. Patsaeva
Photonics 2023, 10(6), 672; https://doi.org/10.3390/photonics10060672 - 9 Jun 2023
Cited by 2 | Viewed by 1280
Abstract
Along the shoreline of the White Sea, due to the post-glacial uplift of the coast, some water bodies with stable stratification have been formed. They have been classified as meromictic as they are at different stages of isolation from the Sea. As separation [...] Read more.
Along the shoreline of the White Sea, due to the post-glacial uplift of the coast, some water bodies with stable stratification have been formed. They have been classified as meromictic as they are at different stages of isolation from the Sea. As separation progresses, significant changes occur in the water column, including the composition of chromophoric dissolved organic matter (CDOM) and the structure of the aquatic microbial community. In this work, we searched for optical proxies of euxinia (anoxic conditions with accumulated hydrogen sulfide) in the water column of the meromictic lagoon on Zeleny Cape. The lagoon is separated from the White Sea basin by a shallow threshold that completely isolates the lagoon during low tide, but marine water enters the lagoon during high tide. The ecosystem in the lagoon is characterized by the marine salinity of water and a high organic matter content in the bottom water and sediments. In this study, spectral methods were used to obtain the depth distribution of CDOM, chlorophyll, and bacteriochlorophyll in the lagoon with strong water stratification and euxinic conditions in the bottom water. The measured optical CDOM characteristics were compared with hydrochemical data (water salinity, Eh, pH, dissolved oxygen), phytoplankton (oxygenic phototrophs), and green sulfur bacteria (anoxygenic phototrophs) distribution along the water column. The spectroscopic methods showed to have the advantages of not requiring water sample pre-treatment and allowing rapid sensing of CDOM and photosynthetic pigments at each horizon. Full article
(This article belongs to the Special Issue Micro Fluorescence Detectors/Sensors and Their Applications)
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11 pages, 4211 KiB  
Article
Feasibility Simulation of 3D Benchtop Multi-Pinhole X-ray Fluorescence Computed Tomography with Two Novel Geometries
by Binqiang Ye, Luzhen Deng, Shanghai Jiang, Sijun Cao, Ruge Zhao and Peng Feng
Photonics 2023, 10(4), 399; https://doi.org/10.3390/photonics10040399 - 3 Apr 2023
Viewed by 1713
Abstract
In this work, we developed and validated two novel imaging geometries of benchtop multi-pinhole X-ray fluorescence computed tomography (XFCT) systems with Geant4 Toolkit. One of the Monte Carlo (MC) models utilized a fan beam source to illuminate a single slice of the object, [...] Read more.
In this work, we developed and validated two novel imaging geometries of benchtop multi-pinhole X-ray fluorescence computed tomography (XFCT) systems with Geant4 Toolkit. One of the Monte Carlo (MC) models utilized a fan beam source to illuminate a single slice of the object, a detector and a multi-pinhole collimator to image each slice’s X-ray fluorescence (XRF). The other model consisted of a cone-beam X-ray source (designed as a 5 mm wide fan beam to reduce simulation time) to scan the whole object, two detectors and two multi-pinhole collimators to image the emissions. The phantom used in the simulations included four sections, each with three cone-shaped gold nanoparticle (GNP) inserts (5 mm in height, 3 mm in diameter across the top) with center-to-center distances of 4 mm, 4.5 mm and 4.86 mm. The GNPs concentration was 0.1 wt. %, 0.3 wt. %, 0.5 wt. % and 0.7 wt. %, respectively. The diameter of the multi-pinhole collimator was 1 mm. Performance was evaluated for pinhole-detector-distance (PDD) of 5 cm, 3.5 cm and 2.5 cm, and the results for different object layers and for single pinhole and multi-pinhole (9 pinholes) imaging were compared. The data showed that results worsened with decreasing GNPs insert diameters and with decreasing PDD (object-pinhole-distance was fixed). The multi-pinhole configurations performed better than a single pinhole. The detection limit for the first multi-pinhole operation was 0.21 wt. %; the second was 0.24 wt. %. Detection limits for the single pinhole were 0.32 wt. % and 0.35 wt. %, respectively. The first MC model could acquire 2D slice images of the object without rotation and the second MC model could image the 3D object efficiently. These two novel multi-pinhole systems could potentially provide a bioimaging modality for nanomedical applications. Full article
(This article belongs to the Special Issue Micro Fluorescence Detectors/Sensors and Their Applications)
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11 pages, 4057 KiB  
Communication
Extensible LED-Induced Integrated Fluorescence Detection Module for Quantitative Analysis of Lucigenin Concentration
by Xiaoguang Qi, Xianglong Hao, Muzi Zhang, Lili Jiang, Wenyue Gao and Chi Wu
Photonics 2023, 10(4), 392; https://doi.org/10.3390/photonics10040392 - 1 Apr 2023
Cited by 1 | Viewed by 1461
Abstract
We developed an extensible LED-induced fluorescence detection module with a highly integrated and ultra-compact structure. A target-oriented design methodology was used to demonstrate the module’s optimal design. Lucigenin solution was used as a test sample in evaluation trials to demonstrate the module’s quantitative [...] Read more.
We developed an extensible LED-induced fluorescence detection module with a highly integrated and ultra-compact structure. A target-oriented design methodology was used to demonstrate the module’s optimal design. Lucigenin solution was used as a test sample in evaluation trials to demonstrate the module’s quantitative fluorescence detection capability. Results showed that the integrated module has an outstanding linear response in the range of 0–1 μmol·L−1, with sensitivity and limit of detection (LOD) of 0.1692 V/μmol·L−1 and 0.03 μmol·L−1, respectively. Statistical analyses showed that our integrated module has extremely high repeatability and accuracy, i.e., the values of Pearson’s correlation coefficient and root-mean-square error exceeded 0.9995 and 1.8‰, respectively. More importantly, the integrated module possesses favorable extensibility and can realize on-demand rapid fluorescence-signal detection of other targets using appropriate parameter combinations. This module offers new opportunities for reliable, cost-effective and easy-to-use fluorescence-signal detection, especially in resource-constrained fluorescence detection applications. Full article
(This article belongs to the Special Issue Micro Fluorescence Detectors/Sensors and Their Applications)
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12 pages, 2010 KiB  
Article
Real-Time Chlorophyll-a Pigment Monitoring of Chlamydomonas reinhardtii in a Controlled Environment Using Pulsed LED Fluorescence LiDAR System
by Jumar G. Cadondon, Edgar A. Vallar, Tatsuo Shiina and Maria Cecilia D. Galvez
Photonics 2023, 10(2), 144; https://doi.org/10.3390/photonics10020144 - 30 Jan 2023
Cited by 3 | Viewed by 1821
Abstract
The real-time chlorophyll-a pigment monitoring of C. reinhardtii is studied using our developed LED fluorescence light detection and ranging (LiDAR) system. It features a portable set-up that uses a pulsed LED module with an excitation wavelength of 385 nm. We were able to [...] Read more.
The real-time chlorophyll-a pigment monitoring of C. reinhardtii is studied using our developed LED fluorescence light detection and ranging (LiDAR) system. It features a portable set-up that uses a pulsed LED module with an excitation wavelength of 385 nm. We were able to monitor the different growth phases of C. reinhardtii with specific cultivation parameters. The developed fluorescence LiDAR system showed the linear correlation of its chlorophyll-a signal with the optical density and EEM fluorescence measurements at 680 nm emission wavelength. Water quality and weather parameters were also measured, which explains the variation in the growth dynamics of C. reinhardtii during the sampling period. The results from the monitoring demonstrated a different technique that can be used in estimating algal biomass in the environment. Full article
(This article belongs to the Special Issue Micro Fluorescence Detectors/Sensors and Their Applications)
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