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Advances in Food Analytical Methods
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Advances in Food Analytical Methods

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Food Chemistry".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 2262

Special Issue Editor

Dr. Yahui Guo

E-Mail Website
Guest Editor
School of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi 214122, China
Interests: food safety and quality control; foodborne hazards detection; development of natural preservatives and colorants
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The continuous evolution of the global food industry demands innovative and precise analytical methods to ensure food safety, authenticity, nutritional quality, and traceability. This Special Issue of Molecules, titled “Advances in Food Analytical Methods,” aims to showcase recent progress in the development and application of novel analytical techniques for food components, contaminants, additives, and functional compounds. We welcome original research and review articles on topics including but not limited to chromatography, mass spectrometry, spectroscopy, biosensors, chemometrics, and emerging AI-based approaches. Studies focusing on rapid, on-site, and high-throughput analysis are particularly encouraged. This Special Issue offers a valuable platform for researchers and practitioners to share breakthroughs that contribute to improved food quality monitoring and regulatory compliance.

Dr. Yahui Guo
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food analysis
  • food component
  • food contaminant
  • food additive
  • functional compound
  • chromatography
  • mass spectrometry
  • spectroscopy
  • biosensor
  • chemometrics
  • AI-based approach
  • food safety
  • food authenticity
  • nutritional quality
  • food traceability

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Published Papers (3 papers)

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Research

23 pages, 9524 KB  
Article
Fluorimetric Determination of Eosin Y in Water Samples and Drinks Using Deep Eutectic Solvent-Based Liquid-Phase Microextraction
by Sofia Kakalejčíková, Yaroslav Bazeľ, Mária Drábiková and Maksym Fizer
Molecules 2025, 30(16), 3334; https://doi.org/10.3390/molecules30163334 - 10 Aug 2025
Viewed by 624
Abstract
An environmentally friendly and highly sensitive analytical method for the determination of the dye Eosin Y (EY) was developed utilizing vortex-assisted liquid–liquid microextraction based on deep eutectic solvents (DESs), combined with fluorescence detection (LPME-FLD). The extraction efficiencies of conventional solvents and various DES [...] Read more.
An environmentally friendly and highly sensitive analytical method for the determination of the dye Eosin Y (EY) was developed utilizing vortex-assisted liquid–liquid microextraction based on deep eutectic solvents (DESs), combined with fluorescence detection (LPME-FLD). The extraction efficiencies of conventional solvents and various DES systems, composed of tetrabutylammonium bromide (TBAB) and alcohols (hexanol, octanol, and decanol) in different ratios, were systematically compared. DFT calculations provided insights into the most stable forms of EY in solvents of varying polarity. Theoretical Hansen solubility parameters and the COSMO-RS solvation model were applied to assess extraction efficiency. Hansen parameters were obtained via semiempirical PM7 calculations, while BP86/def2-TZVPD DFT computations were employed within the openCOSMO-RS framework. The developed method exhibited a linear calibration range between 0.1 and 130 µg·L−1, with a high correlation coefficient (R2 = 0.9982). The limit of detection (LOD) was established at 0.028 µg·L−1. Method precision and repeatability were confirmed over two days, with relative standard deviations (RSDs) ranging from 1.1% to 2.7% and with recoveries between 99.0% and 106.2%. The proposed analytical approach was successfully applied to the determination of EY in real water samples, demonstrating both its practical applicability and alignment with green chemistry principles. Full article
(This article belongs to the Special Issue Advances in Food Analytical Methods)
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14 pages, 2090 KB  
Article
A Carbon Nanofiber Electrochemical Sensor Made of FeMn@C for the Rapid Detection of Tert-Butyl Hydroquinone in Edible Oil
by Yan Xiao, Yi Zhang, Zhigui He, Liwen Zhang, Tongfei Wang, Tingfan Tang, Jiaxing Chen and Hao Cheng
Molecules 2025, 30(13), 2725; https://doi.org/10.3390/molecules30132725 - 25 Jun 2025
Cited by 1 | Viewed by 494
Abstract
Overuse of tert-butylhydroquinone (TBHQ) as a food antioxidant has the potential to pollute the environment and threaten human health. Therefore, it is imperative to develop precise and rapid methods to detect TBHQ in food products. In this study, Fe- and Mn-doped Prussian blue [...] Read more.
Overuse of tert-butylhydroquinone (TBHQ) as a food antioxidant has the potential to pollute the environment and threaten human health. Therefore, it is imperative to develop precise and rapid methods to detect TBHQ in food products. In this study, Fe- and Mn-doped Prussian blue analogs (FeMn-PBAs) were prepared by co-precipitation, FeMn-PBAs/PAN was prepared by electrostatic spinning, and a novel FeMn@C/CNFs composite was prepared by carbonization in nitrogen. Bimetallic FeMn doping has been shown to reduce vacancy defects and enhance the structural stability of PBA. Furthermore, electrostatic spinning has been demonstrated to reduce the agglomeration of PBA nanoparticles, which are electrode-modifying materials with high stability and good electrical conductivity. The morphological and structural characteristics of the FeMn@C/CNF composites were examined using scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The electrochemical behavior of tert-butyl hydroquinone in FeMn@C/CNFs was studied by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and chronocoulometry (CC). The results demonstrate that the sensor exhibits excellent repeatability, reproducibility, and anti-interference capabilities. The prepared electrochemical sensor can be effectively utilized for the detection of TBHQ in food samples such as soybean and peanut oil samples, proving its strong potential for practical applications. Full article
(This article belongs to the Special Issue Advances in Food Analytical Methods)
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14 pages, 3394 KB  
Article
Single-Port Fluorescence Immunoassay for Concurrent Quantification of Live and Dead Bacteria: A Strategy Based on Extracellular Nucleases and DNase I
by Yuhan Wang, Han Dong, Hang Yu, Shaofeng Yuan, Hideya Kawasaki, Yahui Guo and Weirong Yao
Molecules 2025, 30(6), 1374; https://doi.org/10.3390/molecules30061374 - 19 Mar 2025
Viewed by 625
Abstract
Bacteria are the primary culprits of global foodborne diseases, making bacterial detection one of the most critical aspects of food safety. The quantification of viable and dead bacteria is typically achieved through distinct methodologies, such as culture-based methods and molecular biological techniques. These [...] Read more.
Bacteria are the primary culprits of global foodborne diseases, making bacterial detection one of the most critical aspects of food safety. The quantification of viable and dead bacteria is typically achieved through distinct methodologies, such as culture-based methods and molecular biological techniques. These approaches often have non-overlapping requirements in terms of sample pre-treatment and detection equipment. However, in this presented work, bacterial extracellular nucleases and DNase I were utilized to achieve the simultaneous quantification of both live and dead bacteria in a single well of a microplate. The detection limits of the method for live and dead bacteria are estimated to be 7.13 × 105 CFU/mL and 3.54 × 105 CFU/mL, respectively. In the application of detecting bacteria in pickled pork stewed bamboo shoot soup, the detection limit for live bacteria can be reduced to as low as 102 CFU/mL within 24 h after enrichment cultivation. Full article
(This article belongs to the Special Issue Advances in Food Analytical Methods)
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