Fluorescent Materials with Excellent Biocompatibility and Their Application in Bio-Sensing, Bio-Imaging (3rd Edition)

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 578

Special Issue Editors


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Guest Editor
School of Biomedical Engineering, Hainan University, Haikou, China
Interests: fluorescent material; luminescence material; fluorescent probe; fluorescent sensor; biosensor; fluorescent imaging; bioimaging; photosensitizer
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
School of Biomedical Engineering, Hainan University, Haikou, China
Interests: fluorescent material; luminescence material; fluorescent probe; fluorescent sensor; biosensor; fluorescent imaging; bioimaging; photosensitizer
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, Qingdao 266071, China
Interests: fluorescent material; luminescence material; fluorescent probe; fluorescent sensor; biosensor; fluorescent imaging; bioimaging; photosensitizer
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Qingdao Haiwan Science and Technology Industry Research Institute Co., Ltd., Qingdao, China
Interests: fluorescent material; luminescence material; fluorescent probe; fluorescent sensor; biosensor; fluorescent imaging; bioimaging; photosensitizer
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Fluorescent material is an extensively studied issue in the bio-field because it is easy to functionalize and tune fluorescence colour. In previous decades, major progress in the synthesis, characterization, and application of fluorescent materials has been accomplished, along with the recent development of nano-, biobased, sensor, imaging, and high-performance material-based technologies. These advanced technologies promote the application of fluorescent materials in a wide range of applications, for instance, bio-sensing, bio-labelling, bio-tracing, bio-imaging, diseases diagnosis, and therapy, etc. To advance the use of fluorescent materials in the bio-field, the development of biocompatible fluorescent materials (BFM) has become increasingly relevant. The intention of this research topic is to describe the biocompatible fluorescent materials (BFM), the recent breakthroughs in this field, and their application in the bio-field. Our attention will be focused on: (i) the fabrication of novel fluorescent detection devices for bio-objects; and (ii) applications in bio-field, bio-sensing, bio-tracing, bio-imaging and diseases diagnosis and therapy, etc. We hope that this research topic will attract the attention of academic and industrial researchers who are interested in the development of biocompatible fluorescent materials (BFM) and their biological applications. Our goal is to stimulate ideas, methods, and technologies related to chemistry, biology, materials science, medicine, bioscience, and electronics in this exciting area.

We welcome manuscripts from diverse aspects of fluorescent materials, including but not limited to the following:

  • Synthesis and design of novel fluorescent materials with excellent biocompatibility;
  • Preparation of fluorescent materials, including materials synthesis and purification;
  • Multi-scale techniques and morphological studies on biocompatible fluorescent materials;
  • Investigation of fluorescent materials structure-property relationships;
  • Applications in cell-labelling, tumour labelling and therapy, bio-sensing, bio-imaging, etc.;
  • The fabrication of novel fluorescent detection devices for bio-object.

Dr. Yalong Wang
Prof. Dr. Mingqiang Zhu
Dr. Deteng Zhang
Dr. Meng Zheng
Guest Editors

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Keywords

  • fluorescent material
  • luminescence material
  • fluorescent probe
  • fluorescent sensor
  • biosensor
  • fluorescent imaging
  • bioimaging photosensitizer

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Published Papers (1 paper)

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Research

14 pages, 1256 KB  
Article
A First Case of Fluorescence Polarization Biosensor-Based Assay for Rapid Monitoring of Protein API Content in Tablet Dosage Forms: Detection of Lysozyme in Tablets
by Svetlana M. Filimonova, Ksenia S. Balyklova, Dmitry O. Zherdev, Sergei A. Eremin, Liliya I. Mukhametova, Vadim B. Krylov and Nikolay E. Nifantiev
Biosensors 2025, 15(11), 724; https://doi.org/10.3390/bios15110724 - 1 Nov 2025
Viewed by 430
Abstract
Protein-based APIs represent a big group of modern therapeutics. Their characterization involves complex analytical protocols which require special methods, especially in the case when the protein drug is included into tablet dosage forms. Although the fluorescence polarization assay (FPA) is not currently regulated [...] Read more.
Protein-based APIs represent a big group of modern therapeutics. Their characterization involves complex analytical protocols which require special methods, especially in the case when the protein drug is included into tablet dosage forms. Although the fluorescence polarization assay (FPA) is not currently regulated by many national Pharmacopeias, it represents a promising approach for protein drug standardization, considering their rapid, sensitive, and automatable detection suitable for high-throughput analysis and real-time quality control. To evaluate the applicability of FPA for the analysis of protein drugs in tablets, the quantifying of lysozyme in tablet dosage forms was studied by this method with the use of a fluorescently labeled synthetic chitooligosaccharide tracer. It was shown that this approach overcomes the limitations of the conventional turbidimetric assay of lysozyme determination, which is labor-intensive and relies on unstable reagents. Measurements were performed with both portable and stationary fluorescence polarization readers. Commercial tablets from five manufacturers containing lysozyme (20 mg) and pyridoxine hydrochloride (10 mg) together with other excipients were analyzed. The FPIA method showed a linear range of 5.0–70 µg/mL, with specificity confirmed by the absence of interference from excipients. Accuracy, evaluated by standard addition (10–20 mg), yielded recoveries of 100.2–106.0%. Placebo spiked with lysozyme at 80–120% of nominal content demonstrated recoveries of 98.0–100.1%, with RSD (n = 6) not exceeding 13.7%, indicating good precision. The developed method enables reliable lysozyme quantification in tablets, offering speed, simplicity, and robustness, and shows its suitability for the routine quality control of protein-containing dosage forms including the enzyme ones. Full article
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