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Fluorescence Assay for the Determination of d-Panthenol Based on Novel Ring-Fused 2-Pyridone Derivative

by 1,*, 2 and 1
1
Department of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Kraków, Poland
2
Department of Analytical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Kraków, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8386; https://doi.org/10.3390/ijms21218386
Received: 25 October 2020 / Revised: 2 November 2020 / Accepted: 6 November 2020 / Published: 9 November 2020
(This article belongs to the Section Molecular Pharmacology)
Herein, a novel fluorescent method for the determination of d-panthenol (DP) level in solutions with no separate hydrolysis step has been revealed based on the utilization of citric acid (CA) as a derivatizing agent. Consequently, the essential parameters of the derivatization process were established, resulting in the development of sensitive, repeatable, and accurate determination of panthenol. The method was approved, and its usefulness in characterizing the concentration of DP in pharmaceutical formulations and selectivity in the determination of DP were validated. The chemical structure of the new fluorophore formulating in the reaction in DP with CA, i.e., 6-oxo-3,4-dihydro-2H,6H-pyrido[2,1-b][1,3]oxazine-8-carboxylic acid (ODPC), was elucidated using detailed NMR experiments: one-dimensional (1H, 13C) as well as two-dimensional NMR spectra (1H-1H COSY, 1H-13C HSQC, 1H-13C HMBC, 1H-15N HSQC, 1H-15N HMBC). View Full-Text
Keywords: d-panthenol; citric acid; fluorescence; derivatization; ring-fused 2-pyridones d-panthenol; citric acid; fluorescence; derivatization; ring-fused 2-pyridones
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MDPI and ACS Style

Kasprzyk, W.; Świergosz, T.; Koper, F. Fluorescence Assay for the Determination of d-Panthenol Based on Novel Ring-Fused 2-Pyridone Derivative. Int. J. Mol. Sci. 2020, 21, 8386. https://doi.org/10.3390/ijms21218386

AMA Style

Kasprzyk W, Świergosz T, Koper F. Fluorescence Assay for the Determination of d-Panthenol Based on Novel Ring-Fused 2-Pyridone Derivative. International Journal of Molecular Sciences. 2020; 21(21):8386. https://doi.org/10.3390/ijms21218386

Chicago/Turabian Style

Kasprzyk, Wiktor, Tomasz Świergosz, and Filip Koper. 2020. "Fluorescence Assay for the Determination of d-Panthenol Based on Novel Ring-Fused 2-Pyridone Derivative" International Journal of Molecular Sciences 21, no. 21: 8386. https://doi.org/10.3390/ijms21218386

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