Cucurbit downy mildew, caused by the oomycete Pseudoperonospora cubensis, is a devastating disease of cucurbit crops requiring rapid detection for effective management. Current molecular diagnostics often rely on complex laboratory infrastructure, li...
PoRV is a significant etiological agent of neonatal diarrhea in piglets, resulting in substantial economic losses within the global swine industry due to elevated mortality rates and reduced productivity. To address the urgent need for accessible and...
Rathayibacter toxicus is a toxigenic bacterial pathogen of several grass species and is responsible for massive livestock deaths in Australia and South Africa. Due to concern for animal health and livestock industries, it was designated a U.S. Select...
Glyphosate is the most widely used herbicide worldwide, but many current detection methods rely on lab-based chromatography, requiring costly equipment and expert users. Here, we describe a low-cost, field-deployable fluorescence biosensing platform...
A laboratory-based lateral flow (LF) test that utilizes up-converting reporter particles (UCP) for ultrasensitive quantification of Schistosoma circulating anodic antigen (CAA) in urine is a well-accepted test to identify active infection. However, t...
African Swine Fever (ASF) control is severely limited by a diagnostic gap, as laboratory-based quantitative polymerase chain reaction (qPCR) is highly sensitive but slow, whereas field-deployable immunochromatographic assays (ICAs) are rapid but unre...
For millennia humans have benefitted from application of the acute canine sense of smell to hunt, track and find targets of importance. In this report, canines were evaluated for their ability to detect the severe exotic phytobacterial arboreal patho...
Lactococcus garvieae is a major bacterial pathogen responsible for lactococcosis outbreaks in aquaculture, resulting in substantial economic losses worldwide. Accurate identification of L. garvieae remains challenging because of its genetic similarit...
Toxoplasmosis is a zoonotic disease with veterinary and public health importance worldwide. Toxoplasma gondii infection in cetaceans is an indicator of land-to-sea oocyst pollution. However, there is a critical knowledge gap within the distribution o...
Chestnut blight caused by Cryphonectria parasitica poses a major threat to chestnut plantations worldwide. Rapid, field-deployable diagnostic tools are essential for effective disease surveillance and management. Here, we developed and validated an e...
The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threa...
The pervasive contamination of aquatic environments by microplastic particles necessitates the development of rapid, cost-effective and field-deployable detection methodologies to complement established but laboratory-bound spectroscopic techniques s...
Rapid transmission and high mortality rates caused by the SARS-CoV-2 virus showed that the best way to fight against the pandemic was through rapid, accurate diagnosis in parallel with vaccination. In this context, several research groups around the...
The domestic pig (Sus scrofa domesticus) serves as an intermediate host for two Sarcocystis species: the non-zoonotic S. miescheriana and the zoonotic S. suihominis, both of which threaten animal and human health and contribute to economic losses in...
Injuries and disorders affecting the knee joint are very common in athletes and older individuals. Passive and active vibration methods, such as acoustic emissions and modal analysis, are extensively used in both industry and the medical field to dia...
Pathogenic Vibrio species, including Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus, are major foodborne and environmental pathogens that threaten public health. Rapid and accurate detection is crucial for clinical diagnostics, food...
Sweet potato (Ipomoea batatas (L.) Lam) is an important global food crop, but its production is threatened by numerous viral pathogens. More than 30 RNA and DNA viruses have been reported worldwide, making rapid and accurate detection essential for d...
Enteric viruses are a major cause of waterborne infections due to their high environmental stability and extremely low infectious dose. Current molecular diagnostic methods, while accurate, often depend on thermal cycling and centralized laboratory f...
Rapid and reliable on-site identification of body fluids is essential in forensic and field diagnostic applications. Commercial kits provide only single results and often suffer from cross-reactivity, while conventional microarrays offer multiplex ca...
African Swine Fever (ASF) control is severely hampered by the reliance on slow, laboratory-bound diagnostics. While rapid, field-deployable lateral flow assays (LFAs) are urgently needed, the comparative performance of key single-antigen targets rema...
This study presents a mobile-based sperm analysis system featuring a user-friendly, droplet-loaded microfluidic chip that enables non-specialist users to perform the rapid and accurate quantitative evaluation of boar semen directly on the farm. The i...
Nanosurface energy transfer (NSET) has emerged as a pivotal mechanism in nanobiophotonics, facilitating the development of highly sensitive biosensors with extended dynamic ranges. Unlike conventional Förster resonance energy transfer, NSET exhi...
Dengue fever remains a major mosquito–borne disease worldwide, with over 400 million infections annually and a high risk of severe complications such as dengue hemorrhagic fever. The disease is prevalent in tropical and subtropical regions, whe...
The Queensland prawn production industry is worth >$87 million annually and the 2016 White Spot Disease (WSD) outbreak caused >$25 million in losses and complete destruction of all livestock on eight farms in the Logan River region. Whilst the...
Canine monocytic ehrlichiosis (CME), caused by Ehrlichia canis, requires rapid, field-deployable diagnostics in endemic regions. This study developed and evaluated a recombinase polymerase amplification (RPA) assay for rapid isothermal detection of E...
Xanthomonas gardneri is one of the causal agents of bacterial spot (BS), an economically important bacterial disease of tomato and pepper. Field-deployable and portable loop-mediated isothermal amplification (LAMP)-based instruments provide rapid and...
Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper control measure...
The COVID-19 pandemic exposed profound vulnerabilities in global diagnostic infrastructures, highlighting the urgent need for rapid, sensitive, and field-deployable biosensors to manage future outbreaks (Disease X). Ionic liquids (ILs), characterized...
Ascochyta blight (AB), caused by the necrotrophic fungus [Ascochyta rabiei (Pass.) Labr.], is one of the most destructive diseases of chickpea (Cicer arietinum L.), causing yield losses of up to 100% under favorable conditions. The pathogen possesses...
The COVID-19 pandemic has shown that understanding the genomics of a virus, diagnostics and breaking virus transmission is essential in managing viral pandemics. The same lessons can apply for plant viruses. There are plant viruses that have severely...
Bovine Viral Diarrhoea (BVD) is an infectious disease caused by the Bovine Viral Diarrhoea Virus (BVDV), a member of the genus Orthopestivirus. The disease remains endemic across Australian beef and dairy production systems, imposing a multi-million-...
Bovine respiratory disease (BRD) is a multifactorial syndrome and a leading cause of morbidity and economic loss in global cattle production. Next-generation sequencing (NGS) platforms, including Illumina and Oxford Nanopore Technologies (ONT), have...
Antibody-based lateral flow assay (LFA) is a quick and inexpensive tool used to detect pathogens in field samples, especially in hard-to-reach remote areas that may have limited access to central laboratories during an outbreak or surveillance. In th...
The threat of plant diseases in economically significant crops of the Solanaceae family, especially tomatoes and potatoes, is a significant challenge to global food security, highlighting the necessity of fast and convenient diagnostic methods. This...
Point-of-care testing (POCT) offers a transformative approach to diagnostics by enabling rapid and accurate results at or near the site of patient care. This is especially valuable in critical care, emergency settings, and resource-limited areas. How...
Background: Monkeypox (MPX), caused by the Monkeypox virus (MPOX) of the Orthopoxvirus genus, has re-emerged as a significant global health threat. Once confined to Central and West Africa, the 2022–2025 multi-country outbreaks, predominantly c...
After three years of the SARS-CoV-2 pandemic, the demand for developing field-deployable point-of-care (PoC) molecular diagnostic tests has increased. Although RT-qPCR is the molecular diagnostic gold standard and is accurate, it is not readily appli...
The co-circulation of respiratory viruses, including SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV), represents a significant global health challenge that requires rapid, accurate, and differential diagnosis to support infection con...
Fascioliasis, a globally prevalent zoonosis, severely threatens public health and livestock security. Current diagnostic approaches, hindered by the need for sophisticated instrumentation and specialized expertise, are inadequate for on-site surveill...
West Nile virus (WNV) is a mosquito-borne flavivirus of growing importance for both human and equine health in Europe. Horses are highly susceptible to neurological disease and, because they share ecological exposure with humans, they represent valua...
Avian influenza viruses (AIV) cause severe economic losses in poultry production and pose zoonotic threats, necessitating rapid, field-deployable diagnostics. While real-time PCR is the gold standard, its use is limited in resource-constrained settin...
Foodborne pathogens remain a major global concern, demanding rapid, accessible, and determination technologies. Conventional methods, such as culture assays and polymerase chain reaction, offer high accuracy but are time-consuming for on-site testing...
Seoul virus (SEOV), an etiological agent for hemorrhagic fever with renal syndrome, poses a significant public health threat worldwide. This study evaluated the feasibility of a mobile Biomeme platform for facilitating rapid decision making of SEOV i...
Plant diseases destroy 20–40% of global food production annually, posing a critical threat to food security for a projected population of 9.7 billion by 2050. Conventional diagnostic approaches relying on expert visual assessment are slow, cost...
Newcastle disease (ND) is a highly contagious and economically significant viral infection that affects poultry globally, with recurrent outbreaks occurring even among vaccinated flocks in Egypt. Caused by the Newcastle disease virus (NDV), the disea...
The global swine industry suffers persistent economic losses and health challenges due to major viral pathogens such as African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV),...
Background: In 2023, tuberculosis (TB) caused 1.25 million deaths globally, remaining a leading infectious killer. Central Asia and Southern Caucasus face high TB burdens, particularly Mongolia. This review synthesizes TB prevalence data and diagnost...
COVID-19, caused by SARS-CoV-2, has created unprecedented global health challenges, necessitating rapid and reliable diagnostic strategies. The spike (S) protein, particularly its S1 subunit, plays a critical role in viral entry, making it a prime bi...
The widespread and persistent occurrence of micropollutants—such as pesticides, pharmaceuticals, heavy metals, personal care products, microplastics, and per- and polyfluoroalkyl substances (PFAS)—has emerged as a critical environmental a...
The current COVID-19 pandemic has highlighted the power, speed, and simplicity of point-of-care (POC) diagnostics. POC diagnostics are available for a wide range of targets, including both drugs of abuse as well as performance-enhancing drugs. For ph...
of 5