Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Statement
2.2. Chemicals and Materials
2.3. Virus and Clinical Samples
2.4. DNA Extraction
2.5. LAMP Primers
2.6. Benchtop LAMP Amplification
2.7. Benchtop qPCR Amplification
2.8. Microfluidic Chip for Real-Time-LAMP
2.9. The Analytical Sensitivity
2.10. Detection of Nucleic Acids from Clinical Samples
3. Results
3.1. ILTV LAMP Performs on Par with qPCR
3.2. Detection of ILTV with Our Microfluidic Chip and Real-Time LAMP
3.3. Clinical Performance of Our Assay
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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El-Tholoth, M.; Bai, H.; Mauk, M.G.; Anis, E.; Bau, H.H. Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip. Animals 2021, 11, 3203. https://doi.org/10.3390/ani11113203
El-Tholoth M, Bai H, Mauk MG, Anis E, Bau HH. Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip. Animals. 2021; 11(11):3203. https://doi.org/10.3390/ani11113203
Chicago/Turabian StyleEl-Tholoth, Mohamed, Huiwen Bai, Michael G. Mauk, Eman Anis, and Haim H. Bau. 2021. "Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip" Animals 11, no. 11: 3203. https://doi.org/10.3390/ani11113203