Sensors for Detection of Virus and Bacteria

A special issue of Biosensors (ISSN 2079-6374).

Deadline for manuscript submissions: 20 December 2025 | Viewed by 746

Special Issue Editor

Institute for Future Sciences & Hengyang Medical College, University of South China, Hengyang 421001, China
Interests: biosensing and molecular detection; medical artificial intelligence; microfluidic

Special Issue Information

Dear Colleagues,

Rapid and accurate detection of viruses and bacteria is crucial for infectious disease prevention, food safety, and environmental monitoring. However, current detection technologies face challenges such as insufficient sensitivity and specificity, sample complexity, high costs, operational convenience, and limitations in on-site applications. So, this special issue “Sensors for Detection of Virus and Bacteria” aims to explore the latest advancements in biosensing technology for virus and bacteria detection. The scope of submissions includes, but is not limited to:

  • Biosensors based on enzymes, antibodies, nucleic acid aptamers, CRISPR, etc.;
  • Electrochemical, biophotonic, raman spectroscopy, mass spectrometry and other biosensing technologies;
  • Applications of microfluidic chips, bio-MEMS, and nanomaterials in biosensing;
  • Biosensors integrating isothermal amplification, PCR and derived molecular detection methods;
  • Wearable, portable, and label-free biosensing technologies for rapid on-site detection;
  • Biosensor technologies combined with AI, big data, and the Internet of Things.

Dr. Zhu Chen
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bacteria and viruses
  • nanomaterials
  • molecular diagnostics
  • microfluidics and microsystem
  • artificial intelligence
  • electrochemical biosensors
  • optical chemical biosensor

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Published Papers (1 paper)

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Research

12 pages, 1256 KB  
Article
Rapid On-Site Detection of Pseudomonas aeruginosa via ecfX-Targeted Loop-Mediated Isothermal Amplification
by Xuliang He, Meimei Zeng, Wentao Bai, Ziyan Tang, Jianhua Ding and Zhu Chen
Biosensors 2025, 15(11), 750; https://doi.org/10.3390/bios15110750 - 7 Nov 2025
Viewed by 406
Abstract
Pseudomonas aeruginosa (PA) is a significant pathogen of clinical concern that is frequently associated with multidrug resistance, leading to respiratory tract, wound, and hospital-acquired infections. To enable rapid and accurate detection, we developed a fluorescence-based loop-mediated isothermal amplification (LAMP) method, targeting the PA-specific [...] Read more.
Pseudomonas aeruginosa (PA) is a significant pathogen of clinical concern that is frequently associated with multidrug resistance, leading to respiratory tract, wound, and hospital-acquired infections. To enable rapid and accurate detection, we developed a fluorescence-based loop-mediated isothermal amplification (LAMP) method, targeting the PA-specific ecfX gene. Among ten primer sets designed, the optimal set (EC2) was identified, and reaction conditions were optimized (Bst polymerase 320 U/mL, Mg2+ 8 mM, dNTP 1.4 mM, inner/outer primer ratio 1:8, 64 °C, 20 min). The assay demonstrated a detection limit that was comparable to a real-time polymerase chain reaction and immunochromatographic assays, but with a markedly reduced turnaround time. No cross-reactivity was observed with non-PA pathogens, and reproducibility tests confirmed high stability. In addition, the reliability of the results was further verified using 60 standard bacterial strains, and the feasibility of the assay was validated with 2 real soil samples and 1 water sample. This LAMP method offers a simple, rapid, and sensitive tool for on-site detection of PA, with potential applications in clinical diagnostics and public health surveillance. Full article
(This article belongs to the Special Issue Sensors for Detection of Virus and Bacteria)
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