Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography. Continuous affinity capture of adeno-associated viru...
Affinity towards diversity (ATD) is the tendency to appreciate the dynamic variety of biophysical and socio-cultural scenarios. ATD may reveal a human predisposition to conserve the diversity necessary to guarantee the preservation of human and natur...
We characterized the structural determinants of phospholamban (PLB) and sarcolipin (SLN) self-assembly, using experimental and computational assays. PLB and SLN are transmembrane peptides that regulate contractility via phosphorylation-dependent regu...
Recent advancements in high-speed communications and high-capacity computing systems have contributed to major growth in the data volume of databases. Data mining is a crucial part of information retrieval; it is often termed as database knowledge di...
Multi-drone collaborative perception aims to address single-drone viewpoint limitations. The existing matching and association methods based on visual features and spatial topology rely heavily on detection, making it challenging to associate targets...
Gold(III) porphyrin presents an attractive alternative to the use of, for example, cisplatin in chemotherapy. However, approaches that allow to selectively target cancer cells are highly sought. Many plant and mammalian lectins have been shown to bin...
The adenosine A2A receptor (A2AAR) is a class A G-protein-coupled receptor (GPCR). It is an immune checkpoint in the tumor micro-environment and has become an emerging target for cancer treatment. In this study, we aimed to explore the effects of can...
Background: Anthrax is a serious disease caused by Bacillus anthracis (B. anthracis) with a very high mortality when the spores of B. anthracis are inhaled (inhalational anthrax). Aerosolized B. anthracis spores can be used as a deadly bioweapon. Vac...
We propose a new approach to extend the notion of commutator and Lie algebra to algebras with ternary multiplication laws. Our approach is based on the ternary associativity of the first and second kinds. We propose a ternary commutator, which is a l...
The serine/threonine-protein kinase, Akt1, plays an important part in mammalian cell growth, proliferation, migration and angiogenesis, and becomes activated through phosphorylation. To monitor phosphorylation of threonine 308 in Akt1, we developed a...
The binding affinity of ligands for their receptors is determined by their kinetic and thermodynamic binding properties. Kinetic analyses of the rate constants of association and dissociation (kon and koff, respectively) of antihistamines have sugges...
This research aims to determine the similarities in groups of people to build a film recommender system for users. Users often have difficulty in finding suitable movies due to the increasing amount of movie information. The recommender system is ver...
Recombinant adeno-associated virus (rAAV) vectors have evolved as one of the most promising technologies for gene therapy due to their good safety profile, high transduction efficacy, and long-term gene expression in nondividing cells. rAAV-based gen...
Our purpose is to study a space
In this paper, we construct an affine model of a Riemann surface with a flat Riemannian metric associated to a Schwarz–Christoffel mapping of the upper half plane onto a rational triangle. We explain the relation between the geodesics on this Riemann...
Major histocompatibility complex class I-like related gene A (MICA) is the most polymorphic non-classical HLA gene. MICA proteins are expressed at low levels on the surface of normal cells but are highly expressed on the surface of tumor cells. Its m...
This paper surveys results related to well-known works of B. Plotkin and V. Remeslennikov on the edge of algebra, logic and geometry. We start from a brief review of the paper and motivations. The first sections deal with model theory. In the first p...
In this paper, the sampling and reconstruction problems in function subspaces of
Reaction of bis(2-picolyl)amine (BPA) with Ni(II) salt yielded [(BPA)NiCl2(H2O)] (NiBPA). The Ni(II) in NiBPA bound to a BPA ligand, two chloride, and one aqua ligands. Because most medications inhibit biological processes by binding to a specific pr...
Oral vaccination against bacterial pathogens that infect via the gastrointestinal tract is highly desirable for both economic reasons and the supposed benefits of local mucosal immunity. However, the majority of oral vaccine trials in humans result i...
The success of antibody therapeutics is strongly influenced by their multifunctional nature that couples antigen recognition mediated by their variable regions with effector functions and half-life extension mediated by a subset of their constant reg...
Two zinc(II) complexes, DBZ and DBZH4, that have (ZnN3S2) cores and differ in the bridging mode of the ligating backbone, effectively bind to BSA. The binding affinity varies as DBZ > DBZH4 and depends on the ligand structure. At low concentration...
Adeno-associated viruses (AAVs) have emerged as promising tools for gene therapy due to their safety and efficacy in delivering therapeutic genes or gene editing sequences to various tissues and organs. AAV serotype 9 (AAV9), among AAV serotypes, sta...
Skin health depends on the balance of the commensal microbiota. Excessive proliferation of Staphylococcus aureus contributes to inflammatory skin diseases, such as atopic dermatitis, whereas Staphylococcus epidermidis helps maintain skin homeostasis....
Generalized Modules for Membrane Antigens (GMMA) are outer membrane vesicles derived from Gram-negative bacteria that can be used to design affordable subunit vaccines. GMMA have been observed to induce a potent humoral immune response in preclinical...
Calmodulin (CaM) is a small, multifunctional calcium (Ca2+)-binding sensor that binds and regulates the open probability of cardiac ryanodine receptor 2 (RyR2) at both low and high cytosolic Ca2+ concentrations. Recent isothermal titration calorimetr...
Aim: to clarify the association between political party affinity and fear of conventional and nuclear war in Germany. Methods: data were used from a nationally representative online survey (in terms of age bracket, sex and state; n = 3091 individuals...
In order to deepen the understanding of the role and regulation mechanisms of prokaryotic global transcription regulators in complex processes, including virulence, the associations between the affinity and binding sequences of Mycobacterium tubercul...
Nutrition during pregnancy influences perinatal outcomes and predispositions to chronic diseases. A prospective cohort study was carried out with the objectives of describing the dietary patterns in the pregnant population in the city of Quito, Ecuad...
The nucleolus organizer regions (NORs) demonstrate differences in genomic dispersion and transcriptional activity among all organisms. I postulate that such differences stem from distinct genomic structures and their interactions from chromosome obse...
T cells are critical for a functioning adaptive immune response and a strong correlation exists between T cell responses and T cell receptor (TCR): peptide-loaded MHC (pMHC) binding. Studies that utilize pMHC tetramer, multimers, and assays of three-...
The fundamental aim of drug design in research and development is to invent molecules with selective affinity towards desired disease-associated targets. At the atomic loci of binding surfaces, systematic structural variations can define affinities b...
Affinity electrophoresis is an important technique that is widely used to separate and analyze biomolecules in the fields of biology and medicine. Both quantitative and qualitative information can be gained through affinity electrophoresis. Affinity...
The identification of weak-affinity ligands targeting membrane proteins is of great interest in Fragment-Based Drug Design (FBDD). Recently, miniaturized weak affinity chromatography (WAC) has been proposed as a valuable tool to study interactions be...
CCCTC-binding factor (CTCF) is an architectural protein that organizes 3D genome architecture by binding to tens of thousands of genomic loci in a typical human cell type. Although DNA-binding affinity contributes to CTCF occupancy in vivo, the princ...
Macrocyclic imine phycotoxins are an emerging class of chemical compounds associated with harmful algal blooms and shellfish toxicity. Earlier binding and electrophysiology experiments on nAChR subtypes and their soluble AChBP surrogates evidenced co...
The goal of gene therapy is to treat diseases through gene editing and modification of gene expression. Viral vectors, antisense oligonucleotides (ASO) and RNA interference (RNAi) are some of the gene therapies under development. DNAzymes, or deoxyri...
Finding affine parameters for null geodesics is often of considerable physical importance, especially when studying null geodesics or dealing with conservation laws and/or averaged energy conditions. But explicitly finding null affine parameters is a...
For a self-affine iterated function system (IFS)
Immunological synapse (IS) formation is a key event during antigen recognition by T cells. Recent experimental evidence suggests that the affinity between T cell receptors (TCRs) and antigen is actively modulated during the early steps of TCR signali...
We investigated the short sequences involving Omicron 21K and Omicron 21L variants to reveal any possible molecular mimicry-associated autoimmunity risks and changes in those. We first identified common 6mers of the viral and human protein sequences...
Dengue virus (DENV) has four serotypes that complicate vaccine development. Envelope protein domain III (EDIII) of DENV is a promising target for therapeutic antibody development. One EDIII-specific antibody, dubbed 1A1D-2, cross-reacts with DENV 1,...
Ligand-protein interactions are usually studied in complex media that also contain lipids. This is particularly relevant for membrane proteins that are always associated with lipid bilayers, but also for water-soluble proteins studied in in vivo cond...
Background/Objectives: Sustainability discussions are increasingly highlighting the environmental and health impacts of meat production and consumption. The study aimed to analyze how meat attachment influences the intention to reduce meat consumptio...
Background: Metastatic-site frequencies reflect both how extensively a cancer spreads and how commonly an organ is involved across cancers. We assessed whether cancer–organ affinity beyond these overall effects is reproducible between autopsy a...
(1) Background: The folate receptor (FR) is a target for cancer treatment and detection. Expression of the FR is restricted in normal cells but overexpressed in many types of tumors. Folate was conjugated with peptides for enhancing binding affinity...
In this paper, we classify three-dimensional Lorentzian Lie groups on which Ricci tensors associated with Bott connections, canonical connections and Kobayashi–Nomizu connections are Codazzi tensors associated with these connections. We also classify...
The effects of temperature on the operation of two ion-coupled cotransporters of the SLC6A family, namely rat GAT1 (SLC6A1) and KAAT1 (SLC6A19) from Manduca sexta, have been studied by electrophysiological means in Xenopus laevis oocytes expressing t...
Melittin is an anti-microbial peptide (AMP) and one of the most studied membrane-disrupting peptides. There is, however, a lack of accurate measurements of the concentration-dependent kinetics and affinity of binding of melittin to phospholipid membr...
Adenosine receptors are largely distributed in our organism and are promising therapeutic targets for the treatment of many pathologies. In this perspective, investigating the structural features of the ligands leading to affinity and/or selectivity...
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