We identify a tonal contour in Turkish that expresses universal quantification. We show that the distribution of this contour is restricted to noun phrases modified by relative clauses and that it expresses universal quantification over situations ra...
The α-amylase/trypsin inhibitors (ATIs) are discussed as being responsible for non-celiac wheat sensitivity (NCWS), besides being known as allergenic components for baker’s asthma. Different approaches for characterization and quantificat...
In the precision medicine era of cystic fibrosis (CF), therapeutic interventions, by the so-called modulators, target the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The levels of targetable CFTR proteins are a main variable i...
It has been shown that Relative Humidity (RH) provokes dimensional displacement detectable directly from surfaces using holographic interferometry. RH variations constitute a physical environmental load that drives organic materials to a constant equ...
Background: One of the current problems in the field of metabolomics is the difficulty in integrating data collected using different equipment at different facilities, because many metabolomic methods have been developed independently and are unique...
κ-casein (κ-CN) is one of the key components in bovine milk, playing a unique role in the structuration of casein micelles. It contains in its chemical structure up to sixteen amino acid residues (mainly serine and threonine) susceptible...
Quantitative polymerase chain reaction (qPCR) is a widespread method to quantify RNA or DNA. The results are reported as cycle of quantification (Cq), scaled to absolute numbers of copies or relative to reference genes. The reported Cq values of the...
Quantifying metabolites from various biological samples is necessary for the clinical and biomedical translation of metabolomics research. One of the ongoing challenges in biomedical metabolomics studies is the large-scale quantification of targeted...
(1) Background: Mass spectrometry-based quantitative proteome profiling is most commonly performed by label-free quantification (LFQ), stable isotopic labeling with amino acids in cell culture (SILAC), and reporter ion-based isobaric labeling methods...
Potato (Solanum tuberosum L.) is an essential crop for food and industrial production, but its cultivation in low-temperature regions is challenging. Therefore, using proteomics quantification technology based on isobaric tags for relative and absolu...
Metabolic fingerprinting by mass spectrometry aims at the comprehensive, semiquantitative analysis of metabolites. Isotope dilution, if successfully implemented, may provide a more reliable, relative quantification. Therefore, the 13C labeled yeast e...
Plant anthocyanin biosynthesis is well understood, but the regulatory mechanism in purple foliage tea remains unclear. Using isobaric tag for relative and absolute quantification (iTRAQ), 815 differential proteins were identified in the leaves of Zij...
A “two-line hybrid system” was developed, previously based on thermo-sensitive cytoplasmic male sterility in Aegilops kotschyi (K-TCMS), which can be used in wheat breeding. The K-TCMS line exhibits complete male sterility and it can be u...
Cytoplasmic male sterility (CMS) where no functional pollen is produced has important roles in wheat breeding. The anther is a unique organ for male gametogenesis and its abnormal development can cause male sterility. However, the mechanisms and regu...
A novel biodegradable food packaging material based on cassava thermoplastic starch (TPS) and polybutylene adipate terephthalate (PBAT) blends containing food preservatives was successfully developed using blown-film extrusion. This active packaging...
Adventitious root (AR) formation, which is controlled by endogenous and environmental factors, is indispensable for vegetative asexual propagation. However, comprehensive proteomic data on AR formation are still lacking. The aim of this work was to s...
The β-thalassemias are an increasing challenge to health systems worldwide, caused by absent or reduced β-globin (HBB) production. Of particular frequency in many Western countries is HBBIVSI−110(G > A) β-thalassemia (HGVS nam...
Follicle-stimulating hormone (FSH) is a glycohormone synthesized by adenohypophysis, and it stimulates ovulation in women and spermatogenesis in men by binding to its receptor (FSHR). FSHR is involved in several mechanisms to transduce intracellular...
A recent increase in the importance of Rare Earth Elements (REEs), proportional to advancements in modern technology, green energy, and defense, has urged researchers to look for more sophisticated and efficient exploration methods for their host roc...
Five potential reference genes for RT-qPCR application, namely histone H3, beta-actin, GAPDH, ubiquitin and 18S rRNA, were evaluated for normalization of gene expression in four selected tissues (liver, kidney, thyroid and abdominal fat). Tissues wer...
Coral reefs function as submerged breakwaters providing wave mitigation and flood-reduction benefits for coastal communities. Although the wave-reducing capacity of reefs has been associated with wave breaking and friction, studies quantifying the re...
Bone mineral density (BMD) varies with aging and both systemic and local diseases; however, such evidence is lacking in feline medicine. This may be due to the need for general anesthesia in cats for direct BMD measurements using dual-energy X-ray ab...
Cervical cancer is one of the top malignancies in women around the globe, which still holds its place despite being preventable at early stages. Gynecological conditions, even maladies like cervical cancer, still experience scrutiny from society owin...
Dissolved oxygen (DO) is deeply involved in preserving the life of cellular tissues and human beings due to its key role in cellular metabolism: its alterations may reflect important pathophysiological conditions. DO levels are measured to identify p...
Grapevine leafroll-associated virus 3 (GLRaV-3) is a graft-transmissible virus present in every viticultural region of the world and poses a large threat to grapevine production. Frequent coinfections with other viruses, the large number of grapevine...
Identifying relatively weak areas is of great significance for improving the seismic reliability of structures. In this paper, a modal strain energy decomposition method is proposed, which can realize the decoupling of the comprehensive modal strain...
In mass spectrometry-based metabolomics, the differences in the analytical results from different laboratories/machines are an issue to be considered because various types of machines are used in each laboratory. Moreover, the analytical methods are...
The development of systematic coarse-grained mesoscopic models for complex molecular systems is an intense research area. Here we first give an overview of different methods for obtaining optimal parametrized coarse-grained models, starting from deta...
Detection of the Janus Kinase-2 (JAK2) V617F mutation is a diagnostic criterion for myeloproliferative neoplasms, and high levels of mutant alleles are associated with worse outcomes. This mutation is usually tested on blood DNA by allele-specific qP...
Maternal obesity is associated with impaired maternal metabolism and affects the developmental programming of the fetus. The placenta is dysfunctional when exposed to an obese intrauterine environment and can transduce and mediate detrimental materna...
Cultural heritage objects, including traditional Chinese polychrome paintings on architectures (Caihua) and wooden architectural components, frequently exhibit surface defects that are highly sensitive to environmental factors, resulting in progressi...
The mechanical properties of cellular materials are critical to their performance and must be accurately determined through both analytical and numerical methods. These approaches are essential not only for understanding material behavior but also fo...
Plant–pathogen interactions are complex biological processes characterized by dynamic changes in genes expression. In molecular plant pathology research, RT-qPCR has proven to be a valuable tool for investigating plant–pathogen interactio...
Accurate interpretation of qPCR data continues to present significant challenges, particularly at low target concentrations where technical variability, stochastic amplification, and efficiency fluctuations confound quantification. The widespread ass...
Staphylococcus aureus is a pathogen known to cause a wide range of infections. To find new targets for identification and to understand host–pathogen interactions, many studies have focused on surface proteins. We performed bacterial-cell surfa...
Peptide pools consist of short amino acid sequences and have proven to be versatile tools in various research areas in immunology and clinical applications. They are commercially available in many different compositions and variants. However, unlike...
Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective and sensitive techniques in gene expression assay, for which selection of reference genes is a prerequisite. In teleost species, such as Chinese perch, the expres...
Salinity is one of the major abiotic stress factors that limit plant growth and crop yield worldwide. To understand the molecular mechanisms and screen the key proteins in response of sugar beet (Beta vulgaris L.) to salt, in the present study, the p...
This study aimed to quantify the absolute and relative data across a typical microcycle (MC) in female professional soccer players utilizing a match day minus (MD-) approach. Ten players (24.7 ± 2.6 years) from an elite female Portuguese team...
Carbonic anhydrase inhibitors (CAIs) are prescription drugs also used in doping to dilute urine samples and tamper with urinalyses. Dorzolamide, brinzolamide, and acetazolamide are prohibited by the World Anti-Doping Agency. Detecting CAIs and their...
High throughput sequencing has emerged as one of the most important techniques for characterizing microbial dynamics and revealing bacteria and host interactions. However, data interpretation using this technique is mainly based on relative abundance...
When iron sulfides are used as aggregate in concrete production, it easily oxidizes to form harmful substances such as sulfates. This results in acid corrosion and internal sulfate attack (ISA), significantly reducing concrete durability. To date, th...
The article defines and explains methods for detecting and locating damage and quantifying its extent on an example of a finite element model of a simple beam. The defined methods are based on a comparison of the decreases in the bending natural freq...
Detection and quantification of viruses in laboratory and clinical samples are standard assays in dengue virus (DENV) studies. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) is considered to be the standard for DENV detect...
Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical approach that enables both the structural determination and precise quantification of small molecules, such as metabolites. However, achieving precise quantification with NMR invo...
Mass spectrometry (MS) is a widely used analytical technique including medical diagnostics, forensic toxicology, food and water analysis. The gold standard for quantifying compounds involves using stable isotope-labeled internal standards (SIL-IS). H...
The purpose of this study is to understand the effect of ground conditions on microbial cementation in cohesionless soils. Since the method of microbial cementation is still at the experimental stage, for its practical use in the field, a number of l...
Resonance enhanced multiphoton ionization (REMPI) is a powerful method for the sensitive determination of polycyclic aromatic hydrocarbons (PAHs) in gaseous mixtures via mass spectrometry (MS). In REMPI, ions are produced by the absorption of at leas...
Scopoletin has previously been reported as a biomarker for the standardization of Paederia foetida twigs. This study is the first report on the determination and quantification of scopoletin using quantitative nuclear magnetic resonance (qNMR) in the...
Game meat products are particularly prone to be adulterated by replacing game meat with cheaper meat species. Recently, we have presented a real-time polymerase chain reaction (PCR) assay for the identification and quantification of roe deer in food....
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