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Int. J. Mol. Sci. 2018, 19(5), 1344; https://doi.org/10.3390/ijms19051344

iTRAQ-Based Proteomics Analyses of Sterile/Fertile Anthers from a Thermo-Sensitive Cytoplasmic Male-Sterile Wheat with Aegilops kotschyi Cytoplasm

College of Agronomy, Northwest A&F University, Yangling 712100, China
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Received: 2 March 2018 / Revised: 26 April 2018 / Accepted: 27 April 2018 / Published: 2 May 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

A “two-line hybrid system” was developed, previously based on thermo-sensitive cytoplasmic male sterility in Aegilops kotschyi (K-TCMS), which can be used in wheat breeding. The K-TCMS line exhibits complete male sterility and it can be used to produce hybrid wheat seeds during the normal wheat-growing season; it propagates via self-pollination at high temperatures. Isobaric tags for relative and absolute quantification-based quantitative proteome and bioinformatics analyses of the TCMS line KTM3315A were conducted under different fertility conditions to understand the mechanisms of fertility conversion in the pollen development stages. In total, 4639 proteins were identified, the differentially abundant proteins that increased/decreased in plants with differences in fertility were mainly involved with energy metabolism, starch and sucrose metabolism, phenylpropanoid biosynthesis, protein synthesis, translation, folding, and degradation. Compared with the sterile condition, many of the proteins that related to energy and phenylpropanoid metabolism increased during the anther development stage. Thus, we suggest that energy and phenylpropanoid metabolism pathways are important for fertility conversion in K-TCMS wheat. These findings provide valuable insights into the proteins involved with anther and pollen development, thereby, helping to further understand the mechanism of TCMS in wheat. View Full-Text
Keywords: anther proteomics; hybrid wheat; isobaric tags for relative and absolute quantification (iTRAQ); thermo-sensitive cytoplasmic male sterility anther proteomics; hybrid wheat; isobaric tags for relative and absolute quantification (iTRAQ); thermo-sensitive cytoplasmic male sterility
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zhang, G.; Ye, J.; Jia, Y.; Zhang, L.; Song, X. iTRAQ-Based Proteomics Analyses of Sterile/Fertile Anthers from a Thermo-Sensitive Cytoplasmic Male-Sterile Wheat with Aegilops kotschyi Cytoplasm. Int. J. Mol. Sci. 2018, 19, 1344.

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