328 Results Found

Oval cells (OCs) is the name of hepatic progenitor cells (HPCs) in rodents. They are a small population of cells in the liver with the remarkable ability to proliferate and regenerate hepatocytes and cholangiocytes in response to acute liver damage....

Background: Nowadays, many commercial kits allow the detection of Cryptosporidium sp. in stool samples after deoxyribonucleic acid (DNA) extraction. Protocols of stool pretreatment have been proposed to optimize oocysts’ DNA extraction. Among t...

Microplastics (MPs) are persistent, globally relevant pollutants that have thus far been rigorously studied in natural waters but have not been as extensively studied in industrial wastewaters. Samples were collected from the forestry industry, waste...

Several common protein extraction protocols have been applied on seaweeds, but extraction yields have been limited. The aims of this study were to further develop and optimize existing extraction protocols and to examine the effect of enzymatic pre-t...

This report is dedicated to development of surface-enhanced Raman spectroscopy (SERS) based analysis protocol for detection of antibiotics in urine. The key step of the protocol is the pretreatment of urine before the detection to minimize background...

This systematic review aims to evaluate the different pretreatments of the zirconia surface and resin cement in order to determine a valid operative protocol for adhesive cementation. Methodologies conducted for this study followed the Prisma (Prefer...

This paper highlights the potential of Sargassum algae, recovered from raw beach seaweed wastes, as a valid source of valuable sodium alginate. Alginate is a biodegradable, highly attractive polysaccharide widely used in food, pharmaceuticals, and bi...

We present a novel integrated device for preparing metaphase chromosomes spread slides (FISHprep). The quality of cytogenetic analysis from patient samples greatly relies on the efficiency of sample pre-treatment and/or slide preparation. In cytogene...

Thoracic pair of mammary glands from steroid hormone-pretreated mice respond to hormones structurally and functionally in organ culture. A short exposure of glands for 24 h to 7,12 Dimethylbenz(a)anthracene (DMBA) during a 24-day culture period induc...

(1) Background: The objective of this in vitro study was to evaluate the impact of different etching times and ethanol pre-treatments on the immediate bond strength of a hydrophilic multi-mode universal adhesive (Clearfil Universal Bond Quick, Kurara...

We investigated four Cerrado plant species, i.e., Cheiloclinium cognatum (Miers) A.C.Sm, Guazuma ulmifolia Lam., Hancornia speciosa Gomes, and Hymenaea stigonocarpa Mart. ex Hayne, against acetaminophen toxicity using an in vitro assay with HepG2 cel...

The objective of this in vitro study was to investigate the effects of dentin pretreatment protocols and thermocycling on the shear bond strength (SBS) of a self-adhesive resin cement (Maxcem elite chroma) on dentin. A total of 168 extracted human th...

Rapid detection of plant infections is crucial for minimising crop loss and optimising management strategies, particularly in the context of climate change. While traditional diagnostic methods provide precise measurements of phytohormones such as sa...

Polymer Electrolyte Fuel Cells (PEFCs) are one of the most promising power generation systems. The main component of a PEFC is the proton exchange membrane (PEM), object of intense research to improve the efficiency of the cell. The most commonly and...

Several viewpoints have been reported regarding the effect of temporary cements, different surface pretreatment protocols before adhesive cementation, and predictive factors. This in vitro study tested if temporary cement, pretreatment of the tooth s...

Lignocellulosic biomass (LB) is recalcitrant to enzymatic hydrolysis due to its compact and complex cell wall structure. To identify the parameters behind LB recalcitrance, experimental data over hydrolysis time must be collected. Here, we describe a...

Lipids are increasingly recognized as bioactive mediators of extracellular vesicle (EV) functions. However, while EV proteins and nucleic acids are well described, EV lipids are insufficiently understood due to lack of adequate quantitative methods....

Background: The outcome for patients with high-risk neuroblastoma remains poor and novel treatment strategies are urgently needed. The RIST protocol represents a novel metronomic and multimodal treatment strategy for high-risk neuroblastoma combining...

Lignocellulosic biomass is an exciting renewable resource for producing sustainable biofuels, thanks to its abundance and low environmental impact. However, its intricate structure makes it tough for enzymes to break it down effectively. Only efficie...

Tobacco use is the leading cause of preventable death worldwide and is highly addictive. Nicotine is the main addictive compound in tobacco, but less is known about other components and additives that may contribute to tobacco addiction. The zebrafis...

Nowadays, many commercial kits allow the polymerase chain reaction (PCR) detection of Cryptosporidium deoxyribonucleic acid (DNA) in stool samples, the efficiency of which relies on the extraction method used. Mechanical pretreatment of the stools us...

This study investigates the effect of GnRHa pretreatment on pregnancy outcomes in artificial endometrial preparation for frozen–thawed embryo transfer (AC-FET) cycles. A systematic review of English language studies published before 1 September...

The retention of laser-sintered cobalt-chromium (Co-Cr)-based crowns were examined after dentin pretreatment with desensitizing paste containing 8% arginine and calcium carbonate (DP-ACC). Forty lower first molars were prepared using a standardized p...

Background: Nanomedicine approaches for cancer therapy face significant challenges, including a poor tumor accumulation, limited therapeutic efficacy, and systemic toxicity. We hypothesized that controlling the clustering of poly(acrylic acid-co-male...

The effect of dentin pretreatment with Desensitizing Paste containing 8% arginine and calcium carbonate on the retention of zirconium oxide (Y-TZP) crowns was tested. Forty molar teeth were mounted and prepared using a standardized protocol. Y-TZP cr...

Low temperature stress at the seedling stage is a major constraint to rice (Oryza sativa) production. Previously developed screening protocols typically germinate seed and establish seedlings under warm constant-temperature conditions that are optima...

The increasing demand for multi-ingredient dietary supplements (MIDS), driven by diverse consumer health needs, has introduced analytical challenges in product testing and quality control. These challenges stem from complex ingredient interactions, f...

This short investigation deals with a review of the tensile strength properties of six different types of nanocellulose films (carboxymethylated, carboxymethylcellulose-grafted, enzymatically pretreated, phosphorylated, sulfoethylated, and alkoxylate...

Chestnut is a highly valuable species at both the ecological and economic levels, but vegetative propagation protocols have not been optimized for mature material due to its recalcitrant behaviour, thus limiting potential biotechnological application...

Membrane fouling is one of the most important considerations in the design and operation of membrane systems as it affects pretreatment needs, cleaning requirements, operating conditions, cost and performance. Given that membrane fouling represents t...

Objectives: Resin-modified glass ionomer cements (RMGIC) are considered excellent restorative materials with unique therapeutic and anti-cariogenic activity. However, concerns exist regarding the use of polyacrylic acid as a dentine conditioner as it...

The micropropagation of hybrid hazelnut (Corylus americana × Corylus avellana) has been limited in its agricultural application due to the lack of efficient procedures for tissue culture and root organogenesis. We established an efficient steri...

Background: Colorectal cancer is a highly prevalent disease, requiring effective strategies for prevention and treatment. The present research aimed to formulate a natural fiber-rich food product (NFRFP) and to evaluate its safety, toxicogenetics, an...

Sustainable phosphorus (P) recovery from sewage sludge is crucial to reconciling the simultaneous shortage and excess of P. In this study, magnetic biochar (MBC) was synthesized and innovatively applied to enhance P recovery as vivianite. The effects...

Anaerobic acidogenic fermentation presents a promising approach for sustainable carbon emission mitigation in livestock waste management, addressing critical environmental challenges in agriculture. This study systematically investigated the synergis...

Somatic embryogenesis (SE) is considered the most effective method for vegetative propagation of Norway spruce (Picea abies L. Karst). For mass propagation, a storage method that is able to handle large quantities of embryogenic tissues (ETs) reliabl...

Malania oleifera Chun & S.K. Lee, an endemic monotypic species that belongs to the family Olacaceae, is under continuous pressure of decline owing to several ecological and physiological factors. The present study aimed to establish an efficient...

Due to epirubicin’s (EPI) narrow therapeutic index and risk of cardiotoxicity, it is critical to monitor concentrations of this drug when being used to treat cancer patients. In this study, a simple and fast magnetic solid-phase microextraction...

Background: This in vitro study evaluated the effects of two different adhesive application approaches (total-etch and self-etch) and gallic acid (GA) pretreatment on the dentin microshear bond strength (μSBS) of a universal adhesive system. Bond...

Alstroemeria spp. and carnation (Dianthus caryophyllus L.) have considerable and increasing economic importance in the floriculture market, therefore breeders carry out intense breeding programs to select new superior varieties. However, poor germina...

Human SH-SY5Y cell line has been used as an in vitro model in neuroscience research. However, many researchers emphasized that there are many differences between differentiated and undifferentiated characteristics of SH-SY5Y. Although Retinoic Acid (...

Currently, human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) are an attractive source of stem cells for cell-based therapy, owing to their ability to undergo self-renewal and differentiate into all mesodermal, some neuroectodermal...

Background. Differentiated thyroid carcinoma (DTC) is characterized by an excellent prognosis with a 10-year survival rate > 90%. However, when DTC develops in a metastatic form, it has been shown to significantly impact patient survival and quali...

Zebrafish, as an emerging model organism, are widely used in in vivo pharmacodynamic and pharmacokinetic studies. Unlike direct chemical analyses that require no sample preparation, most biological samples must undergo preprocessing steps—proce...

Nitric oxide (NO) induces vasodilation in various types of shock. The effect of pharmacological modulation of the NO pathway in anaphylactic shock (AS) remains poorly understood. Our objective was to assess, through a systematic review, whether inhib...

Global energy demand is projected to increase by approximately 28% by 2040, thereby intensifying the urgent need for sustainable alternatives to fossil fuels. This comprehensive review examines the integration of deep eutectic solvent (DES) pretreatm...

To date, some artificial intelligence (AI) methods have exploited Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) to identify finer tumor properties as potential earlier indicators of pathological Complete Response (pCR) in breast canc...

This study explored the effect of glatiramer acetate (GA, 20 mg) on lesion activity using the 1.5 T standard MRI protocol (single dose gadolinium [Gd] and 5-min delay) or optimized 3 T protocol (triple dose of Gd, 20-min delay and application of an o...

The cell finishing process is the final stage in the production of a battery cell. Almost one third of the production costs of a battery cell are related to this part of the production. It includes a series of steps and technologies aimed at optimizi...

Recreational, reclaimed and drinking source waters worldwide are under increasing anthropogenic pressure, and often contain waterborne enteric bacterial, protozoan, and viral pathogens originating from non-point source fecal contamination. Recently,...