625 Results Found

The development of new cell separation technologies has continued as the demand for sorted cell populations for molecular testing increases. The goal is to increase through-put potential and reduce the manual handling of samples required whilst ensur...

The development of biotransformation must integrate upstream and downstream processes. Upstream bioprocessing will influence downstream bioprocessing. It is essential to consider this because downstream processes can constitute the highest cost in bi...

Oleaginous algae are nowadays of significance for industrial biotechnology applications and for the welfare of society. Tremendous efforts have been put into the development of economically feasible and effective downstream processing techniques in a...

Therapeutic monoclonal antibodies (mAb) are used for the treatment of numerous serious diseases, which have led to an increasing demand over the last decades. Increased cell density and mAb titer of the cultivation broth lead to great challenges for...

Ionic liquids (ILs) are considered a green viable organic solvent substitute for use in the extraction and purification of biosynthetic products (derived from biomass—solid/liquid extraction, or obtained through fermentation—liquid/liquid...

This study reports the separation of methyl ethyl ketone (MEK), a relevant compound in the biorefinery context, from aqueous solutions using activated carbons derived from avocado seed biomass. Two synthesis routes were explored via chemical and ther...

Due to high consumption and non-renewable nature of fossil fuels, rapid development of potential renewable energies such as biofuel derived from microalgae is necessary for achieving the goals of sustainable growth and carbon neutrality. However, the...

The focus of this work was to perform a preliminary study on the suitability of commercially available nanofiltration (NF) and reverse osmosis (RO) membranes for the separation of 1,3-propanediol (1,3-PD) post-fermentation solutions. The experiments...

A novel stationary phase for affinity separations is presented. This material is based on sintered borosilicate glass readily available as semi-finished filter plates with defined porosity and surface area. The material shows fast binding kinetics an...

Developing efficient, clean, and sustainable lignocellulose pretreatment technologies is essential for second-generation biofuel production. In this study, we attempted to use downstream-separated binary acetone-water, n-butanol-water, and ethanol-wa...

In multi-lingual, multi-speaker environments (e.g., international conference scenarios), speech, language, and background sounds can overlap. In real-world scenarios, source separation techniques are needed to separate target sounds. Downstream tasks...

This study investigates the effect of Reynolds number on unsteady buffet characteristics of the OAT15A supercritical airfoil under transonic conditions (Ma = 0.73, AOA = 3.5°) using DDES based on the SST k-ω turbulence model coupled with th...

The process of separating stages is crucial for multistage rockets, directly influencing the success of the launch plan. Different separation timing methods alter the flow field structure within the interlevel zone at separation, influencing the sepa...

Given the complex separation mechanisms of the particulate mixture in a hydrocyclone and the uncertain effects of particle size and shape on separation, this study explored the influence of the maximum projected area of particles on the separation ef...

In this paper, a novel flow perturbation model meant to investigate the effects of incoming wind shear on a hypersonic inlet/isolator is presented. This research focuses on the transient shock/boundary layer interaction and shock train flow evolution...

In a compressor, the periodic wake is an inherently unsteady phenomenon that affects the downstream flow conditions and loading distribution. Thus, understanding the physical mechanisms of these unsteady effects is important for eliminating flow loss...

Knowing the droplet-deformation conditions, the droplet-breakup conditions, and the drag force in the interaction between two droplets with a high Reynolds number is of importance for tracking droplet movement in the annular flow field of a gas well....

Crossflow membrane separation was used as a scalable downstream process for the up concentrate of low-molecular-weight peptides and for the removal of salt (NaCl) from Calanus finmarchicus hydrolysate. Membrane processes are increasingly used for var...

To elucidate how rising temperatures influence native amphipods, and how the expansion of wastewater treatment plants (WWTPs) with a fourth purification stage can alter temperature-related effects, studies were conducted in anthropogenically influenc...

We studied how multiple-stresssors in tributaries affect function, diversity, and physical habitat of recipient downstream ecosystems. Using a mesocosm model of a stream network, we manipulated sediment and nutrients individually and in combination i...

Shock wave boundary layer interactions are common to both supersonic and hypersonic inlet flows. Wall-resolved implicit large-eddy simulations of a canonical Mach 2.5 axisymmetric shock wave boundary layer interaction experiment at Glenn Research Cen...

Active flow control of canonical laminar separation bubbles by steady and harmonic vortex generator jets (VGJs) was investigated using direct numerical simulations. Both control strategies were found to be effective in controlling the laminar boundar...

Suction is an important control method in the shock wave and boundary layer interaction (SWBLI). Aimed at the problem of separation bubbles induced at the expansion corners, this study investigates the influence of suction on both the dimensions of b...

Recently, the bioconversion of biomass into biofuels and biocommodities has received significant attention. Although green technologies for biofuel and biocommodity production are advancing, the productivity and yield from these techniques are low. O...

Sweet sorghum juice (SSJ) was evaluated as fermentation substrate for the production of l-lactic acid. A thermophilic Bacillus coagulans isolate was selected for batch fermentations without the use of additional nutrients. The first batch of SSJ (Bat...

In this study, the effect of additional positions of rigid splitter plates on the response characteristics of tandem cylinders at a Reynolds number of 150 and a fixed distance ratio of 5.0 was numerically investigated via the computational fluid dyna...

Lipid extraction from microbial and microalgae biomass requires the separation of oil-rich cells from the production media. This downstream procedure represents a major bottleneck in biodiesel production, increasing the cost of the final product. Flo...

In this study, a biomimetic microfluidic plasma separation device is discussed. The design of the device drew inspiration from in vivo observations of enhanced cell-free layer (CFL) formation downstream of vascular bifurcations. The working principle...

A convex corner models the upper surface of a deflected flap and shock-induced boundary layer separation occurs at transonic speeds. This study uses micro-vortex generators (MVGs) for flow control. An array of MVGs (counter-rotating vane type, ramp t...

The successful development of a lactobionic acid (LBA) bioconversion process on an industrial scale demands the selection of appropriate downstream methodological approaches to achieve product purification once the bioconversion of LBA is completed....

The large meridional expansion turbine stator leads to complex secondary flows and heat transfer characteristics in the blade endwall region, while the upstream tip clearance leakage flow of the rotor makes it more complex in flow and heat transfer....

High centrifugal acceleration and throughput rates of tubular centrifuges enable the solid–liquid size separation and fractionation of nanoparticles on a bench scale. Nowadays, advantageous product properties are defined by precise specifications reg...

Plasma separation is an essential step in blood-based diagnostics. While traditional centrifugation is effective, it is costly and usually restricted to centralized laboratories because it requires relatively expensive equipment, a supply of consumab...

Deflected control surfaces can be used as variable camber control in different flight conditions, and a convex corner resembles a simplified configuration for the upper surface. This experimental study determines the presence of passive vortex genera...

In the present work, direct numerical simulation is employed to investigate the unsteady flow characteristics and energy performance of low-pressure turbines (LPT) by considering the blades aeroelastic vibrations and inflow wakes. The effects of infl...

Vortex generators (VGs) are a passive method by which to alleviate boundary layer separation (BLS). The device-induced streamwise vortices propagate downstream. There is then lift-off from the surface and the vortex decays. The effectiveness of VGs d...

The development of monoclonal antibodies (mAbs) has provided vast opportunities to treat a wide range of diseases from cancer to viral infections. While plant-based production of mAbs has effectively lowered the upstream cost of mAb production compar...

The present study focuses on the impact of the underlying RANS turbulence model in the Zonal Detached Eddy Simulation (ZDES) method when used for secondary flow prediction. This is carried out in light of three issues commonly investigated for hybrid...

The influence of 3 MW Hywind-II wind turbine wakes from an upstream offshore floating wind turbine on a downstream turbine with a separation distance of seven rotor diameters was studied for a site in the Gulf of Maine. The turbines and the platforms...

Magnetic cell separation has become a key methodology for the isolation of target cell populations from biological suspensions, covering a wide spectrum of applications from diagnosis and therapy in biomedicine to environmental applications or fundam...

A dry, low-NO_x (DLN) combustor for a heavy-duty gas turbine using lean premixed technology was studied. A high-fidelity test model was built for the experimental study using particle image velocimetry (PIV). The non-reacting flow in the DLN combustio...

In this article, the optimization of a realistic oil and gas separation plant has been studied. Using Latin Hypercube Sampling (LHS) and rigorous process simulations, surrogate models using Kriging have been established for selected model responses....

Rare earth elements (REE), originally found in various low-grade deposits in the form of different minerals, are associated with gangues that have similar physicochemical properties. However, the production of REE is attractive due to their numerous...

The hydrodynamic performance and wake evaluation results of a cantilever flexible cylindrical triangular array at Re = 9000 and with L/D spacing ratios ranging from 3 to 7 are presented in this paper. The improved delayed separation eddy current simu...

In order to investigate the transport law of an unsteady wake in a downstream cascade channel in a turbine stage environment, this study was based on a self-designed unsteady wake generator, and a low-pressure turbine cascade was the research object....

Background: The Fujiang River, a first-order branch of Jialing River, has for years been separated into six segments by six cascading hydropower stations in its downstream. However, the impact of cascading hydropower stations on its aquatic biota com...

Optically induced dielectrophoresis (ODEP)-based microparticle sorting and separation is regarded as promising. However, current methods normally lack the downstream process for the transportation and collection of separated microparticles, which cou...

Channel reconfiguration is a common but debated method used to restore streams, often causing disturbance and producing subsequent negative impacts on biota. Here, we report results from short-term assessment (i.e., one and three years’ post-restorat...

The downstream processing of natural active molecules remains the most significant cost in the production pipeline. This considerable cost is largely attributed to rigorous chromatographic purification protocols. In an ongoing effort to abate the dep...

Reporter genes have contributed to advancements in molecular biology. Binding of an upstream regulatory protein to a downstream reporter promoter allows quantification of the activity of the upstream protein produced from the corresponding gene. In s...