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13,460 Results Found

  • Review
  • Open Access
13 Citations
3,802 Views
11 Pages

Surgery for Port-Wine Stains: A Systematic Review

  • Giacomo Colletti,
  • Sara Negrello,
  • Linda Rozell-Shannon,
  • Gregory M. Levitin,
  • Liliana Colletti,
  • Luigi Chiarini,
  • Alexandre Anesi,
  • Mattia Di Bartolomeo,
  • Arrigo Pellacani and
  • Riccardo Nocini

28 June 2023

Background: Port-wine stains (PWS) are congenital low-flow vascular malformations of the skin. PWS tend to become thicker and darker with time. Laser therapy is the gold standard and the first-line therapy for treating PWS. However, some resistant PW...

  • Article
  • Open Access
3 Citations
4,575 Views
12 Pages

Effect of Silicon Wafer Surface Stains on Copper-Assisted Chemical Etching

  • Liang Ma,
  • Xiuhua Chen,
  • Chenggui Tang,
  • Shaoyuan Li,
  • Fengshuo Xi,
  • Huayan Lan,
  • Wenhui Ma and
  • Yuanchih Chang

11 April 2023

Silicon wafer slicing is a crucial process during solar cell fabrication, but it often stains the silicon wafer surface. Thus, this work systematically investigated the composition, source, and cleaning method of typical white spot stains on silicon...

  • Article
  • Open Access
2 Citations
2,835 Views
11 Pages

Identification of Benzodiazepine Use Based on Dried Blood Stains Analysis

  • Lucía Fernández-López,
  • Sandra Rodríguez,
  • Alberto Cánovas-Cabanes,
  • Francisco-Javier Teruel-Fernández,
  • Pilar Almela,
  • Juan-Pedro Hernández del Rincón,
  • Javier Navarro-Zaragoza and
  • María Falcón

Biological matrices are typically used in forensic toxicological or pharmacological analysis: mainly blood, vitreous humor or urine. However, there are many cases in which crimes are a consequence of drug intoxication or drug abuse and they are not c...

  • Article
  • Open Access
8 Citations
4,536 Views
16 Pages

In Vitro Evaluation of the Shading Effect of Various Zirconia Surface Stains on Porcelain Crowns

  • Shih-Chieh Lin,
  • Wei-Chun Lin,
  • Yu-Ling Lin,
  • Min Yan and
  • Cheng-Ming Tang

Human teeth display various colors under natural light. Dental restorations, such as zirconia crowns, are generally used to rehabilitate the oral function of patients with tooth loss due to trauma or natural tooth falls. However, significant improvem...

  • Article
  • Open Access
2,656 Views
12 Pages

Efficacy of Biomimetic Hydroxyapatite in the Treatment of Extrinsic Dental Stains in Smokers and Non-Smokers

  • Sarkis Sozkes,
  • Maria Chomyszyn-Gajewska,
  • Agata Dudzik and
  • Iwona Olszewska-Czyz

23 May 2025

Smoking is a major risk factor for a variety of oral diseases. In particular, smoking-induced dental stains have been shown to be more refractory than those in non-smokers. Hydroxyapatite (HAP) is a biomimetic material that has been shown to be helpf...

  • Article
  • Open Access
795 Views
17 Pages

15 October 2025

Washing performance depends on the specific interactions between textiles, stains, detergents, mechanical action, temperature, and time. Its evaluation therefore requires a fundamental and practical understanding of the effects of the washing paramet...

  • Article
  • Open Access
36 Citations
5,198 Views
13 Pages

18 September 2019

We studied the feasibility of image-based quantitative analysis of foxing stains on collections of old (16th–20th century) European books stored in the Rare Book Library of the Seoul National University in Korea. We were able to quantitatively...

  • Article
  • Open Access
13 Citations
3,828 Views
16 Pages

StainView: A Fast and Reliable Method for Mapping Stains in Facades Using Image Classification in HSV and CIELab Colour Space

  • Marta Torres-Gonzáles,
  • Jónatas Valença,
  • Bruno O. Santos,
  • Ana Silva and
  • Maria P. Mendes

1 June 2023

The new Construction 4.0 paradigm takes advantage of existing technologies. In this scope, the development and application of image-based methods for evaluating and monitoring the state of conservation of buildings has shown significant growth, inclu...

  • Feature Paper
  • Article
  • Open Access
1,638 Views
18 Pages

19 September 2025

Accurate assessment of sperm morphology is essential for artificial insemination using liquid-preserved boar semen. This study compared nine commonly used staining techniques, eosin, eosin–nigrosin, Diff-Quick®, Hemacolor®, Sangodiff-G&...

  • Systematic Review
  • Open Access
2 Citations
3,006 Views
20 Pages

Port-Wine Stains’ Orodental Manifestations and Complications: A Systematic Review

  • Andreea Kui,
  • Marius Negucioiu,
  • Smaranda Buduru,
  • Ana-Maria Condor,
  • Daria Chira,
  • Daniela Cornelia Condor and
  • Ondine Patricia Lucaciu

7 March 2025

Background/Objectives: Port-wine stains (PWSs), also known as naevus flammeus or capillary malformations, are congenital cutaneous lesions, typically located in the head/neck area. The manifestations of PWSs include aesthetic and functional impairmen...

  • Review
  • Open Access
35 Citations
7,850 Views
16 Pages

Pathogenesis of Port-Wine Stains: Directions for Future Therapies

  • Lian Liu,
  • Xiaoxue Li,
  • Qian Zhao,
  • Lihua Yang and
  • Xian Jiang

12 October 2022

Port-wine stains (PWSs) are congenital vascular malformations that involve the skin and mucosa. To date, the mechanisms underlying the pathogenesis and progression of PWSs are yet to be clearly elucidated. The potential reasons for dilated vessels ar...

  • Article
  • Open Access
9 Citations
2,675 Views
16 Pages

Analytical Investigation of Iron-Based Stains on Carbonate Stones: Rust Formation, Diffusion Mechanisms, and Speciation

  • Rita Reale,
  • Giovanni Battista Andreozzi,
  • Maria Pia Sammartino and
  • Anna Maria Salvi

7 February 2023

In cultural heritage, unaesthetic stains on carbonate stones due to their close contacts with metals are of concern for the preservation of sculptures, monumental facades and archeological finds of various origin and antiquities. Rust stains made up...

  • Article
  • Open Access
2 Citations
2,675 Views
16 Pages

5 October 2021

In this work, the creation of a dataset labeled in a pixel-wise manner for the uncommon domain of stain detection on patterned laundry is described. The unique properties of images in this dataset—stains are small and sometimes occur in large amounts...

  • Review
  • Open Access
11 Citations
5,125 Views
23 Pages

5 October 2022

Fungi are the main contaminants of books and archival documents. In addition to their degrading power, offered by various types of lignolytic and cellulolytic enzymes, they can also hue the surface of the paper through the production of pigments. The...

  • Article
  • Open Access
10 Citations
3,512 Views
9 Pages

Blue Laser (450 nm) in the Treatment of Port Wine Stains and Telangiectasia

  • Jacek Szymańczyk,
  • Witold Trzeciakowski,
  • Yurij Ivonyak,
  • Piotr Tuchowski and
  • Janusz Szymańczyk

18 March 2021

This study aimed to test a blue light source for the treatment of port wine stains (PWS) and telangiectasia and to compare this with the application of green and yellow lasers based on data in the literature. A total of 22 patients with PWS were trea...

  • Article
  • Open Access
11 Citations
3,350 Views
14 Pages

Bacterial Nanocellulose Hydrogel for the Green Cleaning of Copper Stains from Marble

  • Erica Sonaglia,
  • Emily Schifano,
  • Mohammad Sharbaf,
  • Daniela Uccelletti,
  • Anna Candida Felici and
  • Maria Laura Santarelli

18 February 2024

Cultural heritage stone materials frequently experience significant discoloration induced by copper corrosion products, especially calcareous stones associated with bronze or copper statues and architectural elements. This alteration originates from...

  • Article
  • Open Access
1 Citations
1,320 Views
32 Pages

11 April 2025

Around the world, a large number of stone artifacts have been exposed to air for long periods of time, showing multiple types of deterioration that have attracted widespread attention. Among them, there is an often overlooked deterioration of stone a...

  • Feature Paper
  • Article
  • Open Access
288 Views
14 Pages

17 December 2025

Stone relics exposed to outdoor environments frequently experience surface deterioration, with red stains being a common and persistent issue. The stains often observed on marble and limestone surfaces arise from complex interactions involving chemic...

  • Review
  • Open Access
9 Citations
12,486 Views
10 Pages

We suggest a novel method that uses artificial blood cells (hemoglobin vesicles, Hb-Vs) as photosensitizers in dye laser treatment (at 595-nm wavelength) for port-wine stains (i.e., capillary malformations presenting as red birthmarks) based on the r...

  • Article
  • Open Access
12 Citations
2,471 Views
19 Pages

Deep learning for the analysis of H&E stains requires a large annotated training set. This may form a labor-intensive task involving highly skilled pathologists. We aimed to optimize and evaluate computer-assisted annotation based on digital dual...

  • Article
  • Open Access
1,863 Views
14 Pages

Tetranuclear Polypyridylruthenium(II) Complexes as Selective Nucleic Acid Stains for Flow Cytometric Analysis of Monocytic and Epithelial Lung Carcinoma Large Extracellular Vesicles

  • Kartika Wardhani,
  • Aviva Levina,
  • Biyun Sun,
  • Haipei Zou,
  • Georges E. R. Grau,
  • F. Richard Keene,
  • J. Grant Collins and
  • Peter A. Lay

Selective staining of extracellular vesicles (EVs) is a major challenge for diagnostic and therapeutic applications. Herein, the EV labeling properties of a new class of tetranuclear polypyridylruthenium(II) complexes, Rubb7-TNL and Rubb7-TL, as phos...

  • Article
  • Open Access
231 Views
19 Pages

9 December 2025

Wood stains are developed to enhance the aesthetic appeal and durability of wood by improving water repellency (WR) and protecting surfaces from biological decay and UV degradation. However, limited information is available on whether these performan...

  • Article
  • Open Access
13 Citations
5,531 Views
25 Pages

StainCUT: Stain Normalization with Contrastive Learning

  • José Carlos Gutiérrez Pérez,
  • Daniel Otero Baguer and
  • Peter Maass

In recent years, numerous deep-learning approaches have been developed for the analysis of histopathology Whole Slide Images (WSI). A recurrent issue is the lack of generalization ability of a model that has been trained with images of one laboratory...

  • Article
  • Open Access
3 Citations
4,425 Views
27 Pages

In Vivo Assessment of Thermosensitive Liposomes for the Treatment of Port Wine Stains by Antifibrinolytic Site-Specific Pharmaco-Laser Therapy

  • Mingjuan Li,
  • M. Ingmar van Raath,
  • Shervin Khakpour,
  • Ahmet Seçilir,
  • Bart C. Sliggers,
  • Xuan Huang,
  • Baoyue Ding,
  • Gert Storm,
  • René R. van der Hulst and
  • Anton I.P.M. de Kroon
  • + 1 author

Antifibrinolytic site-specific pharmaco-laser therapy (SSPLT) is an experimental treatment modality for refractory port wine stains (PWS). Conceptually, antifibrinolytic drugs encapsulated in thermosensitive liposomes are delivered to thrombi that fo...

  • Viewpoint
  • Open Access
1 Citations
1,909 Views
15 Pages

Many current reports in the scientific literature describe novel fluorescent probes intended to provide information on various structures or properties of live cells by using microscopic imaging. Unfortunately, many such reports fail to provide key i...

  • Article
  • Open Access
3 Citations
6,853 Views
21 Pages

13 December 2019

Port wine stains (PWSs) are congenital dermal vascular lesions composed of a hyperdilated vasculature. Purpura represented by local hemorrhage from water vaporization in blood during laser therapy of PWS is typically considered a clinical feedback, b...

  • Article
  • Open Access
20 Citations
11,237 Views
14 Pages

Stain Resistance of Cotton Fabrics before and after Finishing with Admicellar Polymerization

  • Srinivas Hanumansetty,
  • Jayanta Maity,
  • Rod Foster and
  • Edgar A. O’Rear

5 March 2012

Environmental concerns related to perfluoroctanoic acid (PFOA) led to a re-examination of the methods for imparting stain resistance and stain repellency to textiles. Non-PFOA fluoropolymer finishes have been formed on cotton knits by admicellar poly...

  • Article
  • Open Access
2 Citations
5,682 Views
12 Pages

A Simple Procedure for the Evaluation of Bone Vitality by Staining with a Tetrazolium Salt

  • René Schiffner,
  • Juliane Reiche,
  • Steffen Brodt,
  • Olaf Brinkmann,
  • Matthias Bungartz,
  • Georg Matziolis and
  • Martin Schmidt

Presently, no intra-operative method for a direct assessment of bone vitality exists. Therefore, we set out to test the applicability of tetrazolium-based staining on bone samples. The explanted femoral heads of 37 patients were used to obtain either...

  • Article
  • Open Access
1 Citations
2,031 Views
8 Pages

Evaluation of Optimized Toluidine Blue Stain as an Alternative Stain for Rapid On-Site Evaluation (ROSE)

  • Alejandro S. Mendoza,
  • Alaa Afify,
  • Lydia Howell,
  • John Bishop,
  • Aurelia Lauderdale,
  • Stan Seko,
  • Ronelson Hermosilla,
  • Donald York and
  • Kurt B. Schaberg

Background: Rapid on-site evaluation (ROSE) is crucial for improving the diagnostic yield of fine-needle aspiration (FNA) biopsies. Despite recent advances in ROSE, such as telecytology, the rapid stains used in this process have not seen significant...

  • Article
  • Open Access
5 Citations
5,934 Views
14 Pages

Simple Staining of Cells on a Chip

  • Fatma Betul Kosker,
  • Omer Aydin and
  • Kutay Icoz

13 November 2022

Simple staining of cells is a widely used method in basic medical diagnostics, education, and research laboratories. The stains are low-cost, but the extensive consumption results in excessive toxic waste generation. Thus, to decrease the amount of t...

  • Article
  • Open Access
292 Citations
4 Pages

The ability to use chemical staining to discriminate aborted from non-aborted pollen grains has well-known practical applications in agriculture. A commonly used technique for assessing pollen vitality, Alexander’s stain, uses chloral hydrate, phenol...

  • Article
  • Open Access
5 Citations
11,234 Views
8 Pages

Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs

  • Peter O. Forson,
  • Patience B. Tetteh-Quarcoo,
  • John Ahenkorah,
  • Robert Aryee,
  • Esther N. Okine,
  • Emmanuel Afutu,
  • Georgina I. Djameh,
  • Jeffrey Agyapong,
  • Abraham K. Anang and
  • Patrick F. Ayeh-Kumi

23 April 2019

This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine sampl...

  • Article
  • Open Access
2,494 Views
15 Pages

Serous effusion cytology is a pivotal diagnostic and staging tool in clinical pathology, valued for its simplicity and cost-effectiveness. Staining techniques such as Giemsa and Papanicolaou are foundational, yet the search for rapid and efficient al...

  • Protocol
  • Open Access
30 Citations
13,716 Views
15 Pages

A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis

  • James P. Hinton,
  • Katerina Dvorak,
  • Esteban Roberts,
  • Wendy J. French,
  • Jon C. Grubbs,
  • Anne E. Cress,
  • Hina A. Tiwari and
  • Raymond B. Nagle

15 November 2019

Archived Hematoxylin and Eosin (H&E) stained pathology slides are routinely stored to index formalin-fixed paraffin-embedded (FFPE) sample tissue blocks. FFPE blocks are clinically annotated human tumor specimens that can be valuable in studies d...

  • Article
  • Open Access
23 Citations
4,752 Views
23 Pages

SA-GAN: Stain Acclimation Generative Adversarial Network for Histopathology Image Analysis

  • Tasleem Kausar,
  • Adeeba Kausar,
  • Muhammad Adnan Ashraf,
  • Muhammad Farhan Siddique,
  • Mingjiang Wang,
  • Muhammad Sajid,
  • Muhammad Zeeshan Siddique,
  • Anwar Ul Haq and
  • Imran Riaz

29 December 2021

Histopathological image analysis is an examination of tissue under a light microscope for cancerous disease diagnosis. Computer-assisted diagnosis (CAD) systems work well by diagnosing cancer from histopathology images. However, stain variability in...

  • Protocol
  • Open Access
9 Citations
7,752 Views
7 Pages

An Inexpensive Staining Alternative for Gelatin Zymography Gels

  • Christian Wechselberger,
  • Christian Doppler and
  • David Bernhard

Zymography is a widely used electrophoretic method to determine proteolytic activities in samples from various sources. The method is based on copolymerizing a suitable protein substrate within a sodium dodecyl sulfate-polyacrylamide gel. Following e...

  • Article
  • Open Access
5 Citations
3,659 Views
18 Pages

(1) Background: The wet mount staining technique is a simple, economical, and rapid method for detecting parasitic stages. The objective of the current study was to evaluate wet mount diagnostic stains for Cryptosporidium infection in human faecal sa...

  • Article
  • Open Access
7 Citations
3,989 Views
11 Pages

Mold and Stain Resistance of Bamboo Treated with Pyraclostrobin Fungicide

  • Jingpeng Zhang,
  • Mingliang Jiang,
  • Bin Zhang,
  • Yuzhang Wu and
  • Xingxia Ma

17 December 2022

Bamboo is rich in starch and sugars and can be infected by mold and stain fungi, degrading its performance, shortening its service life, and reducing its utilization value. It is crucial to investigate how to protect bamboo against mold and stain fun...

  • Article
  • Open Access
2 Citations
2,775 Views
14 Pages

Underneath the Purple Stain

  • Catarina Pinheiro,
  • Ana Zélia Miller,
  • Patrícia Vaz,
  • Ana Teresa Caldeira and
  • Conceição Casanova

11 December 2022

The Alcobacenses are one of the most important Portuguese collections of Monastic codices. During a campaign study to further advance our knowledge on this important legacy and its state of conservation, a heavily deteriorated codex (ALC.338) stood o...

  • Review
  • Open Access
8 Citations
4,190 Views
17 Pages

The Usefulness of Elastin Staining to Detect Vascular Invasion in Cancer

  • Jeffrey Gonzalez,
  • Hisham F. Bahmad,
  • Stephanie Ocejo,
  • Alvaro Abreu,
  • Meagan Popp,
  • Samantha Gogola,
  • Vielka Fernandez,
  • Monica Recine and
  • Robert Poppiti

17 October 2023

Tumor prognosis hinges on accurate cancer staging, a pivotal process influenced by the identification of lymphovascular invasion (LVI), i.e., blood vessel and lymphatic vessel invasion. Protocols by the College of American Pathologists (CAP) and the...

  • Article
  • Open Access
1,576 Views
15 Pages

12 January 2025

Wood blue staining is one of the most common wood defects, which commonly occurs in rubberwood and Masson pine. It not only affects the appearance of the wood, but also its properties. In this study, rubberwood from Xishuangbanna was examined. During...

  • Communication
  • Open Access
17 Citations
12,058 Views
10 Pages

DNA Comet Giemsa Staining for Conventional Bright-Field Microscopy

  • Andreyan Osipov,
  • Ekaterina Arkhangelskaya,
  • Alexei Vinokurov,
  • Nadezhda Smetaninа,
  • Alex Zhavoronkov and
  • Dmitry Klokov

10 April 2014

This study was undertaken to evaluate the compatibility of Giemsa staining protocol with the comet assay. We showed, for the first time, that DNA comets can be visualized and analyzed using Giemsa staining. We generated DNA damage dose response curve...

  • Guidelines
  • Open Access
3 Citations
5,643 Views
10 Pages

Perls’ Stain Guidelines from the French-Speaking Cellular Hematology Group (GFHC)

  • Camille Lours,
  • Laurane Cottin,
  • Margaux Wiber,
  • Valérie Andrieu,
  • Véronique Baccini,
  • Lucile Baseggio,
  • Chantal Brouzes,
  • Bernard Chatelain,
  • Sylvie Daliphard and
  • Odile Fenneteau
  • + 12 authors

In order to standardize cellular hematology practices, the French-speaking Cellular Hematology Group (Groupe Francophone d’Hématologie Cellulaire, GFHC) focused on Perls’ stain. A national survey was carried out, leading to the pro...

  • Article
  • Open Access
1,016 Views
13 Pages

Evaluation of Ozonized Gel Application for Stain Removal on Dental Composite Resins

  • Paolo Zampetti,
  • Marco Colombo,
  • Maurizio Pascadopoli,
  • Simone Gallo,
  • Claudio Poggio,
  • Jamil Tayybia and
  • Andrea Scribante

Staining removal is an issue of interest in dentistry. Current treatments deal with staining removal on enamel, while few studies concentrate on resin composites. The aim of the current study is to evaluate the efficacy in staining removal of an ozon...

  • Communication
  • Open Access
3 Citations
3,709 Views
10 Pages

Improvements for Tissue-Chopping-Based Immunofluorescence Staining Method of Chloroplast Proteins

  • Lulu Wang,
  • Yajuan Chen,
  • Di Niu,
  • Mingdong Tang,
  • Jinjie An,
  • Shanshan Xue,
  • Xiaomin Liu and
  • Hongbo Gao

13 February 2023

Immunofluorescence staining is a very common method for the subcellular localization study of proteins. A tissue-chopping-based immunofluorescence staining method for chloroplast proteins overcomes the restriction of plant cell wall, makes the operat...

  • Article
  • Open Access
6 Citations
7,963 Views
16 Pages

Obtaining fine neuron morphology and connections data is extraordinarily useful in understanding the brain’s functionality. Golgi staining is a widely used method for revealing neuronal morphology. However, Golgi-Cox-stained tissue is difficult...

  • Article
  • Open Access
10 Citations
5,568 Views
25 Pages

H&E Multi-Laboratory Staining Variance Exploration with Machine Learning

  • Fabi Prezja,
  • Ilkka Pölönen,
  • Sami Äyrämö,
  • Pekka Ruusuvuori and
  • Teijo Kuopio

26 July 2022

In diagnostic histopathology, hematoxylin and eosin (H&E) staining is a critical process that highlights salient histological features. Staining results vary between laboratories regardless of the histopathological task, although the method does...

  • Article
  • Open Access
3 Citations
7,830 Views
16 Pages

Whitening Efficiency of Papain and Bromelain Gels in Removing Dental Stains

  • Stanca Cuc,
  • Codruta Sarosi,
  • Ioan Petean,
  • Amalia Moldovan,
  • Cecilia Bacali and
  • Sorin Claudiu Man

1 February 2025

This study aimed to evaluate the micro-nanostructure and color changes of dental enamel after treatment with new gel formulations containing papain or bromelain. Eighty caries-free, extracted human teeth were randomly divided into two groups (n = 40)...

  • Article
  • Open Access
11 Citations
1,776 Views
20 Pages

Study on the Process Optimization of Peanut Coat Pigment Staining of Poplar Wood

  • Yiqing Qi,
  • Ziqiang Zhang,
  • Yue Sun,
  • Liming Shen and
  • Jianlin Han

8 March 2024

Wood staining is a crucial technique for enhancing the decorative effect of wood. Different mordants and mordant processes can influence the staining effect of wood. In this study, three types of mordants and mordant methods were selected to improve...

  • Article
  • Open Access
4 Citations
2,303 Views
11 Pages

P16/Ki67 Dual Staining in Glandular Cell Abnormalities of the Uterine Cervix

  • Saša Jeromel,
  • Alenka Repše Fokter and
  • Andraž Dovnik

23 April 2024

Very limited information exists about the role of p16/Ki67 dual staining on glandular cells in detecting glandular precancerous lesions and cervical adenocarcinoma. In this study, we investigated the diagnostic accuracy of p16/Ki67 dual staining for...

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