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Oral

Oral — Health, Diseases, Therapies, and Technologies is an international, peer-reviewed, open access journal on oral health published quarterly online by MDPI.

Quartile Ranking JCR - Q3 (Dentistry, Oral Surgery and Medicine)

All Articles (246)

Background: Antimicrobial resistance in oral pathogens drives interest in natural alternatives such as essential oils (EOs). Methods: The chemical composition and in vitro antimicrobial activity of Origanum vulgare, Thymus vulgaris, and Lavandula angustifolia EOs were investigated. Oils were profiled by gas chromatography–mass spectrometry (GC-MS) and tested against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 using the disc diffusion method (triplicate, 1 µL/disc, ~850–950 µg). Results: O. vulgare oil produced the strongest inhibition against C. albicans (18.4 ± 0.5 mm), T. vulgaris was most active E. coli (13.0 ± 0.5 mm), while L. angustifolia showed negligible activity (6–7 mm). All EO inhibition zones were smaller than those of antibiotics. Conclusions: At clinically relevant doses, Oregano and Thyme oils showed modest antimicrobial effects, whereas Lavender was inactive. However, these findings are limited by the use of ATCC strains, small sample size, and reliance on the disc diffusion method, which provides only qualitative data and does not capture biofilm or host interactions. Future studies should include minimum inhibitory concentrations (MIC)/minimum bactericidal concentrations (MBC) assays, biofilm models, and cytotoxicity testing. AI-assisted GC-MS analysis and automated inhibition zone measurement should be considered as future perspectives to improve reproducibility and translational potential.

4 November 2025

Schematic representation of the standardized agar disc diffusion method (Kirby-Bauer) used in this study (Created with AI-assisted design software—ChatGPT version 5.0).

Links Between Dental Anxiety and Preventive Dental Care: A Cross-Sectional Study in an Oral Surgery Practice

  • Rezart Qorri,
  • Nertsa Cunoti and
  • Laura Agnes Ingrid Magerfleisch
  • + 6 authors

Background/Objectives: Dental anxiety has been demonstrated to exert a significant influence on the frequency of treatment, the degree of patient compliance, and the long-term implications for oral health. The objective of this study was to evaluate the extent of dental anxiety experienced by patients attending an oral surgery practice in Germany. In addition, the study sought to assess their preventive dental behaviour and to explore any potential associations between anxiety levels and the utilisation of preventive care services. Methods: The present study comprised 102 consecutive adult outpatients from a private oral surgery practice. The assessment of dental anxiety was conducted using the Corah’s Dental Anxiety Scale (DAS). The behaviour of the subjects in relation to preventative dental care was measured using a series of six standardised items, including the frequency of tooth brushing, tartar removal, and professional tooth cleaning. The data analysis comprised descriptive statistics, chi-square (χ2) tests, multiple linear regression, and one-way analysis of variance (ANOVA). Results: The mean DAS score was 12.69 (SD = 3.36). According to the DAS classification system, 28.4% of patients reported low anxiety, 50.0% moderate anxiety, and 21.6% high dental anxiety. Regression analysis explained 10% of the variance in DAS scores (R2 = 0.128, adj. R2 = 0.100). The analysis revealed that age (β = −0.218, p = 0.035) and lower educational level (β = −0.357, p < 0.001) were associated with higher anxiety scores, whereas sex was not a significant predictor. Preventive dental behaviour, encompassing practices such as tooth brushing, professional tooth cleaning, and tartar removal, did not exhibit significant disparities across anxiety groups (all p > 0.05). Conclusions: While dental anxiety was prevalent among the patient sample, it did not exert a significant influence on preventive dental behaviours. However, patients with lower levels of education and younger age exhibited higher dental anxiety scores. These findings underscore the necessity for targeted educational and preventive strategies in patients with elevated dental anxiety.

3 November 2025

Effect of Adding Chitosan Nanoparticles to Acrylic Resin on Mechanical and Antimicrobial Properties of Removable Dentures: Scoping Review

  • Daniele Morais Dias,
  • Letícia Pena Botelho and
  • Larissa Doalla de Almeida e Silva
  • + 2 authors

Acrylic resin is widely used in removable dental prostheses due to its biocompatibility, low cost, and ease of handling; however, it presents mechanical limitations and a high susceptibility to microbial colonization, particularly by Candida albicans. The incorporation of nanoparticles into polymethyl methacrylate (PMMA) has been investigated as a strategy to mitigate these drawbacks. This scoping review evaluated the impacts of incorporating chitosan (CTS) nanoparticles into PMMA on antimicrobial activity and mechanical properties. A comprehensive search of the PubMed, Scopus, Web of Science, and BVS databases resulted in the retrieval of 1912 records. After removing 557 duplicates and applying the eligibility criteria, 9 in vitro studies were included. Despite methodological heterogeneity, most studies reported enhanced antifungal activity against C. albicans and improvements in mechanical properties, such as microhardness and overall strength, when CTS was incorporated. Thus, CTS appears to be a promising additive for denture base resins, with the potential to reduce denture-associated infections and increase the longevity of prostheses. Nevertheless, standardized methodologies and well-designed in vivo and clinical investigations remain essential to determine optimal concentrations, incorporation techniques, and long-term clinical performance before implementation. A scoping review design was selected due to the exploratory nature of the study and the heterogeneity of available evidence, which precludes direct comparisons and quantitative synthesis.

3 November 2025

Laser Doppler Flowmetry and Continuous Tissue Oxygenation Monitoring: Best of Vitality Tests?

  • Herman J. J. Roeykens,
  • Rani D’haese and
  • Wolfgang Jacquet
  • + 2 authors

This study aimed to explore the added value and usage of laser Doppler flowmetry (LDF) in conjunction with continuous tissue (arterial) oxygen saturation (SO2) monitoring, electrical pulp testing (EPT), cold stimulation (CS) testing, and apical X-rays (RX). LDF data were evaluated in relation to three different scenarios. LDF records of all four upper incisors from 30 randomly selected patients aged 21–40 were analysed in relation to the following scenarios: (a) simultaneous SO2 measurements using a pre-manufactured splint handled by an experienced LDF dentist, (b) EPT, and (c) CS. A total of 120 teeth were analysed, of which 11 were non-vital (7 denervated and 4 traumatised). Data assessment showed the following mean LDF values: vital teeth: 23.6 Perfusion Units (PU), SD 6.3 and SaO2 of 88.7%, SD 17.1. For non-vital teeth, the mean LDF value was 16.1 PU (SD 11.8) and the mean SO2 value was 70.8% (SD 31.9). The standard deviation was found to be twice as high for non-vital teeth as for vital teeth. No direct relationship was found between LDF and SO2 values at low SO2. For vitality discrimination, the ROC curves showed an area under the curve of 0.799 for LDF and 0.643 for SO2. EPT data assessment showed a mean value of 18.1 (SD 19.7) out of a possible score of 0–80. This was distributed as follows: seven non-vital teeth (80/80); 109 vital teeth; and four undecided teeth. This was compared to the LDF and SO2 results. The data assessment showed nine non-vital teeth, 108 vital teeth, and three undecided teeth in comparison to LDF and SO2 results. Conclusion: LDF and SO2 do not complement each other sufficiently in detecting non-vital teeth when the selection criteria are applied. While LDF clearly contributes, the vital or non-vital classification still depends on a combination of X-ray, sensitivity, and vitality tests.

20 October 2025

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Oral - ISSN 2673-6373Creative Common CC BY license