Oral — Health, Diseases, Therapies, and Technologies

Objectives: This study aimed to investigate the influence of saliva on the adhesion of C. albicans to various obturator prosthetic materials. Methods: This in vitro study investigated C. albicans adherence using clinical isolates, including one isolated from an obturator. The adherence of C. albicans cells to heat-cured acrylic, self-cured acrylic, a tissue conditioner, and silicone was measured using static and flow adhesion assays. The effect of pooled saliva from patients receiving radiotherapy or healthy volunteers on C. albicans adherence was determined. The adsorption of salivary proteins to acrylic coupons was investigated using SDS-polyacrylamide gel electrophoresis, Western blotting, and mass spectrometry. Results: It was found that C. albicans adhered to all obturator materials. Saliva was found to approximately double the adhesion of C. albicans to obturator materials, with saliva from patients who had received radiotherapy as part of their cancer treatment tending to increase adhesion more than saliva from healthy volunteers. The protein SPLUNC2 was found to be selectively concentrated by heat- and self-cured acrylic and may contribute to the adhesion of C. albicans to acrylic. Conclusions: This study found that saliva promotes the adhesion of C. albicans, and salivary proteins may play a role in facilitating this process. Adhesion was lower to acrylic-based prosthetic materials than to other materials. This suggests that interim obturators should be made from self-cured acrylic, and definitive obturators should be made from heat-cured acrylic. Full article

Objectives: The aim of this study was to present and describe a digital workflow integrating Digital Smile Design (DSD) with computer-aided design/computer-aided manufacturing (CAD/CAM) and additive manufacturing technologies for the fabrication of dental preparation guides, focusing on workflow feasibility, design reproducibility, and clinical handling. Materials and Methods: A digital workflow was implemented using intraoral scanning and Exocad DentalCAD 3.1 Elefsina software to design dental preparation guides based on digitally planned restorations. Preparation margins, insertion paths, and minimal material thickness were defined virtually. The guides were fabricated using both subtractive (PMMA milling) and additive (stereolithographic-based 3D printing) manufacturing techniques. Post-processing included chemical cleaning, support removal, additional light curing, and manual finishing. The evaluation was qualitative and descriptive, based on visual inspection, workflow performance, and guide adaptation to printed models. Results: The proposed digital workflow was associated with consistent fabrication of preparation guides and predictable transfer of the virtual design to the manufactured guides. Digital planning facilitated clear visualization of preparation margins and insertion axes, supporting controlled and minimally invasive tooth preparation. The workflow demonstrated good reproducibility and efficient communication between clinician and dental technician. No quantitative measurements or statistical analyses were performed. Conclusions: Within the limitations of this qualitative feasibility study, the integration of DSD with CAD/CAM and 3D printing technologies represents a viable digital approach for designing and fabricating dental preparation guides. The workflow shows potential for improving predictability and communication in restorative dentistry. Full article

Objectives: This cross-sectional study aimed to evaluate oral health knowledge, self-perceived oral status, hygiene behaviors, prevalence of dental injuries, and mouthguard use among Croatian basketball players and coaches. Materials and Methods: A total of 414 participants of both sexes, spanning various levels of basketball participation in Croatia, completed an anonymous online questionnaire assessing oral health knowledge, hygiene habits, trauma history, and preventive practices. Data was analyzed using descriptive statistics, Chi-square, Mann–Whitney, and Kruskal–Wallis tests. Results: Participants demonstrated generally poor oral health knowledge, with coaches scoring significantly higher than players (p < 0.05), and knowledge levels varying according to education and socioeconomic status (p ≤ 0.001). A majority reported no prior dental trauma (69.6%), and mouthguard use was notably low (20.8%). While regular toothbrushing was prevalent (87%), the use of dental floss was limited (39.9%). The most reported oral health problems included gingival bleeding (37.9%), dental calculus (35.0%), and tooth sensitivity (34.3%). Conclusions: Despite relatively good hygiene habits, Croatian basketball players demonstrated low oral health knowledge and insufficient preventive practices, particularly regarding mouthguard use. These findings indicate the need for targeted education and preventive strategies within the basketball community. Full article

Objectives: To assess the utility of Microsoft Copilot, a generative AI tool, in providing meaningful differential diagnosis and management strategies comparable with those generated by a board-certified radiologist using cone beam computed tomography (CBCT) studies in maxillofacial disease and thus assess its potential utility to help with the initial provisional diagnostic process. Study Design: A pilot project designed as a single-center, retrospective study using a convenient sample was conducted. De-identified data collected from patient charts in a consistent format was fed to Microsoft 365 Copilot (MCP) to generate a list of meaningful differential diagnosis (DD) and management protocols. Scores ranging of 0–3 were given for 0–3 matches in DD and management protocols, respectively. Results: Proportional analysis showed that the radiologist and Copilot agreed on the DD in 75.2% of cases and 94.6% of cases in management protocols. For biopsy recommendations, the radiologist and Copilot advised biopsy in 33 (89.2%) cases while they did not recommend biopsy in 23 (41.8%) cases. Conclusions: Generative AI platforms at this point may have value in generating DD and management protocols based on maxillofacial CBCT findings. However, the radiologist’s judgement based on clinical context, feature recognition, and critical analysis seemed to outperform MCP. Larger studies with statistical validation are warranted. Full article

Background: Cognitive aging is a physiological process that involves gradual and mild changes in mental functions. When these changes significantly affect cognitive performance, it is considered cognitive decline. Objective: This analytical cross-sectional pilot study examined the association between periodontal status, systemic conditions, and cognitive performance in middle-aged and older adults. Methods: Forty adults aged 35–59 years (n = 20) and ≥60 years (n = 20) from northeastern Mexico were evaluated. Oral assessments included the Modified Gingival Index and detection of Porphyromonas gingivalis and Fusobacterium nucleatum using qPCR. Cognitive function was evaluated with the Mini-Mental State Examination (MMSE), and frailty with the Clinical Frailty Scale (CFS) and Oral Frailty Checklist (OF-5). Systemic medical history and oral hygiene habits were determined using a questionnaire. Results: MMSE scores were lower in older adults compared with middle-aged adults, and the magnitude of the difference was small. The presence of P. gingivalis or F. nucleatum was similar between groups. Frailty indicators were more prevalent in older adults. Logistic regression identified age and frailty-related variables as the strongest predictors of lower cognitive performance, whereas microbiological findings were not significant predictors. Conclusions: Age and frailty indicators, rather than bacterial presence alone, were associated with reduced cognitive performance in this pilot sample. Although no microbiological differences were observed, the findings highlight the need for larger analytical studies incorporating quantitative bacterial load and additional confounders to better understand the oral–systemic–cognitive interactions. Full article

Objectives: This study aimed to comprehensively evaluate and compare the therapeutic effects of Right Angle Maxillary Protraction Appliance (RAMPA) therapy on nasal airway volume in pediatric patients, specifically differentiated by their baseline radiological paranasal sinus status. The objective was to quantify airway volume changes (absolute and percentage) in clear and opacified sinus groups, investigate the influence of age, sex, and treatment duration on these changes, and elucidate potential differences in the underlying mechanisms of airway expansion between groups. Study Design: A retrospective comparative cohort study design was employed. This study includes a “clear sinus group” of 26 patients (mean age: 6.6 years) with radiologically clear sinuses at baseline and an “opacified sinus group” of 20 patients (mean age: 6.8 years) diagnosed with rhinosinusitis and exhibiting significant sinus opacification on baseline CBCT scans. Upper airway volumetric measurements were performed using CBCT scans acquired pre- (T1) and post-treatment (T2), with data analyzed using Invivo 5 software. Results: RAMPA therapy significantly increased upper airway volume in both cohorts. The clear sinus group showed an approximate 18% mean increase (4886.9 mm³ absolute), while the opacified sinus group demonstrated a remarkably greater 61% mean increase (11,192.8 mm³ absolute). This difference was statistically significant. In the clear sinus group, airway volume gain positively correlated with treatment duration (p = 0.0303). Conversely, no significant correlation was found in the opacified sinus group (p = 0.288), suggesting rapid obstruction relief as a dominant mechanism. Sex did not significantly influence outcomes, and age was not a strong independent predictor of volume change magnitude. Conclusions: RAMPA therapy effectively increases upper airway volume in pediatric patients, with a substantially greater effect in those with baseline sinus opacification due to rapid obstruction resolution complementing skeletal changes. The mechanism of action differs by sinus status, with clear sinus patients showing gradual, duration-dependent skeletal adaptation and opacified sinus patients exhibiting immediate, duration-independent gains primarily from sinus clearance. These findings provide crucial insights for tailored clinical decision-making. Full article

Background/Objectives: The purpose of this in vitro study is to evaluate the effect varying palatal vault depths have on the accuracy of complete maxillary denture bases fabricated using additive manufacturing technology. Methods: One hundred complete maxillary denture bases were manufactured on two different digital light processing (DLP) dental 3D printers at five different palatal depths. After manufacturing, the denture bases were post-cured, scanned, and then analyzed in metrology software. Statistically significant differences were determined using two-way ANOVA tests for normally distributed data and the Kruskal–Wallis test for non-normally distributed data. Color deviation maps were used to give clinical relevance to the results. Results: Significant differences were found for both printers among some groups for the different palatal depths. In relation to the negative mean deviation, the data revealed that the NextDent printers were the least accurate (0.047 ± 0.004) in the group with the deepest palate. The positive mean deviation revealed the most deviation (0.077 ± 0.009) in the group with the deepest palate, which was also mirrored in the Asiga printer (0.050 ± 0.002). The color deviation maps revealed areas of positive and negative average deviation in all groups. The effect of the printer model (p = 0.007) and palatal depth (p = 0.04) on negative average deviation was significant. The effect of the interaction of printer and palatal depth was also significant (p = 0.001). Conclusion: Deeper palatal vaults are associated with higher deviation in DLP 3D-printed complete maxillary denture bases manufactured through additive manufacturing. Full article

Objective: This is the first population-based study to separately examine whether preventive and treatment dental visits modify the association between stressful life events and toothache. Psychological stress may alter health-seeking behaviors and pain perception, potentially leading to symptom-driven rather than preventive dental visits. Methods: Cross-sectional data were obtained and analyzed from the 2019 wave of the Japan Gerontological Evaluation Study (JAGES), including 19,314 community-dwelling adults aged 65 years and older. Poisson regression analyses were conducted to examine the associations between stressful life events, dental visit history, and self-reported toothache. Results: Higher stressful life events were associated with greater toothache prevalence (PR = 1.189; 95% CI: 1.117–1.264). Preventive dental visits within the past six months were linked to the lowest risk, whereas recent treatment visits were associated with higher risk. Conclusion: Promoting regular preventive dental visits, particularly among individuals under high stress, may help reduce the likelihood of experiencing toothache among older adults. Full article

Background: The time from mastication to swallowing is used as an indicator of masticatory and swallowing functions. However, there have been no reports on reasonable eating times associated with eating satisfaction. Clarifying the reasonable time for eating and drinking to achieve eating satisfaction will indicate the level of mastication and swallowing functions that contributes to maintaining an individual’s quality of life. Objective: This study aimed to determine the time from food intake to the end of swallowing that is associated with eating satisfaction. Methods: A cross-sectional survey of 437 community-dwelling older people was conducted. Mastication and swallowing times (SST-MST) were measured using the Saku-Saku Test with a 2 g rice cracker. Food intake difficulty and eating satisfaction were evaluated using a questionnaire. The association between the SST-MST and the difficulty in eating food or eating satisfaction was assessed by sensitivity, specificity, Youden index, sensitivity–specificity ratio, positive likelihood ratio, negative likelihood ratio, odds ratio, and 95% confidence interval. Results: Most indices indicated that a cutoff point of 25 s on the SST-MST was associated with not having difficulty in eating food items. Moreover, when the SST-MST cutoff was set to 25 s, all indices showed a favorable association with eating satisfaction. Conclusions: In community-dwelling older people in their 70s and 80s, an SST-MST of about 25 s for 2 g of rice crackers was modestly associated with eating satisfaction. Full article

Background/Objectives: Dental malocclusions are often treated with appliances made of metal alloys. These alloys biodegrade in oral cavity and release toxic metals such as nickel and chromium. This study aimed to assess nickel and chromium content in the saliva of patients with and without fixed metallic orthodontic appliances. Methods: This was a descriptive cross-sectional study aiming to assess nickel and chromium content in saliva. A survey was conducted to record socio-demographic characteristics and clinical signs due to the wearing of fixed metallic orthodontic appliances. A 10 mL saliva sample was used to measure salivary pH and assess nickel and chromium concentrations using atomic emission spectrophotometry. A Student’s t-test compared saliva metal levels in non-wearers and wearers of metal orthodontic appliances. A Chi-square test was used to assess the influence of pH on metal release in patients. Results: A total of 92 participants, divided in two groups; 46 without appliance and 46 wearing appliance were received during the study period. Their mean age was 17.05 ± 6.46 years. Patients’ mean saliva pH was 6.97 ± 0.44. The mean nickel concentration was 4.39 ± 4.01 µg/L in the saliva of non-appliance wearers and 20.41 ± 18.56 µg/L in the saliva of appliance wearers, respectively. The chromium mean concentration was 1.3 ± 1.33 µg/L for non-appliance wearers and 9.38 ± 19.49 µg/L and for appliance wearers. Metal release is influenced by the pH of foods. Conclusions: Metal orthodontic appliances increase the release of nickel and chromium in saliva. It is necessary to monitor the risk of intolerance and optimize treatment duration. Full article

Background/Objectives: Medication-related osteonecrosis of the jaw (MRONJ) is a serious complication of antiresorptive and antiangiogenic drugs with no consensus on optimal treatment. This study aimed to evaluate the clinical outcomes of MRONJ patients managed at a tertiary referral center over a 15-year period. Methods: We conducted a retrospective cohort study of 130 patients diagnosed with MRONJ (per AAOMS criteria) at a single tertiary hospital between 2006 and 2021. Data on demographics, underlying disease, drug history, MRONJ stage, triggering events, and treatment (conservative vs. surgical) were collected from medical records. Treatment outcomes were categorized as complete resolution, stable disease, or progression. Univariate and multivariate logistic regression analyses were performed to identify predictors of resolution. Results: Most patients (84.6%) had an underlying malignancy. The primary causative agents were zoledronic acid (47.7%) and denosumab (30.0%), the most frequent site was the mandible (66.2%), and the main trigger was dental extraction (59.2%). Crude resolution rates were 20.3% for conservative management versus 83.6% for surgical management. Treatment was stage-driven, with surgery common in advanced stages. At 12 months, 43.1% of all patients achieved complete resolution, and 52.3% remained stable. Multivariate analysis confirmed surgical treatment as the only independent predictor of complete resolution (OR = 20.1, 95% CI: 8.1–50). Conclusions: In this cohort, conservative care was generally sufficient for Stage I disease, while surgical intervention was the strongest predictor of healing and provided reliable outcomes for advanced MRONJ. These findings support an individualized, stage-appropriate treatment strategy. Full article

Introduction: This study aimed to evaluate the educational value of YouTube videos presenting information on pediatric orthodontic treatments, specifically clear aligners, fixed braces, and lingual braces. Methods: A cross-sectional analysis was conducted on 150 of the most-viewed English-language YouTube videos (50 per treatment type). Videos were assessed for accuracy, depth of explanation, source type (expert, commercial, or general user), and viewer engagement metrics. Statistical comparisons were made using the Kruskal–Wallis test and Dunn’s post hoc tests with Bonferroni correction. Results: Fixed brace videos demonstrated more accuracy (average score 4.2/5), than both clear aligners (p = 0.03) and lingual braces (p < 0.001), with 60% originating from expert sources. Clear aligner and lingual brace videos had lower accuracy scores (3.8 and 3.5, respectively), reflecting higher proportions of commercial influence (50% and 55%). The findings highlighted the predominance of promotional content in clear aligner and lingual brace videos, raising concerns about potential misinformation. Conclusions: YouTube provides accessible but variable-quality information on pediatric orthodontics. Fixed brace videos offer more reliable educational content, while clear aligner and lingual brace videos are more susceptible to commercial bias. Efforts to promote expert-driven content and implement content verification systems are needed to improve the quality of online orthodontic information for parents and caregivers. Full article

Background/objectives: Zirconia dental implants are increasingly recognised as an alternative to titanium implants due to their biocompatibility and aesthetics. Initially developed as one-piece systems, zirconia implants have evolved into two-piece designs with different platform levels; however, comparative data on their primary and secondary stability– particularly as assessed by resonance frequency analysis (RFA)—and marginal bone dynamics remain limited. This case series aimed to evaluate the implant stability and marginal bone changes of two-piece zirconia implants with bone-level (BL) and tissue-level (TL) platforms in patients missing maxillary premolars. Methods: Thirteen zirconia implants (n = 13; 7 BL, 6 TL; Z5-TL/Z5-BL, Z-Systems, Switzerland) were placed in 11 patients with healed ridges. The implant stability quotient (ISQ) was measured immediately after insertion and before prosthetic loading. Lithium disilicate crowns were cemented after four months, and follow-ups were conducted for an average of 35 months (SD = 12). Results: Initial ISQ values ranged from 73 to 79, increasing to 76–84 at 3–4 months, indicating high implant stability for both BL and TL implants. The extent of marginal bone loss (MBL) after two years was greater around BL implants (mean 0.46 mm) compared to TL implants (mean 0.2 mm), although probing depths and bleeding on probing remained minimal in both groups, with only one TL implant showing gingival recession. Conclusions: Over a short observation period, two-piece zirconia implants with tissue-level platforms appeared to demonstrate superior marginal tissue stability. Further, larger-scale controlled studies are required to confirm these preliminary observations. Full article

Molar incisor hypomineralisation (MIH) is a developmental defect affecting permanent first molars and often the incisors too. Hypomineralised second primary molars (HSPM) have been proposed as potential early indicators of MIH. Aim: The aim was to identify potential aetiological factors associated with MIH and assess their relationship with HSPM in a pilot study. Methods: A cross-sectional case–control study was conducted with 120 patients (60 cases and 60 controls), aged 7–15 years, from the Paediatric Dentistry Postgraduate Programme. MIH was diagnosed following European Academy of Paediatric Dentistry (EAPD) guidelines. Parents completed a structured questionnaire on potential aetiological factors. Results: MIH was significantly associated with maternal smoking during pregnancy (p = 0.013), birth hypoxia (p = 0.013) and the use of amoxicillin and inhalation therapy during infancy (p < 0.001). It was also associated with tonsillitis (p = 0.022), bronchiolitis (p = 0.005) and other respiratory disorders (p = 0.049). HSPM was associated with anaemia and hypotension during pregnancy (p = 0.001), bottle-feeding (p = 0.044) and urinary tract infections (p = 0.003). No statistically significant association was found between MIH and HSPM. Conclusions: This pilot study has identified specific prenatal, perinatal, and postnatal factors associated with MIH and HSPM. The findings emphasise the clinical relevance for early diagnosis and management and highlight the need for studies with larger sample sizes to validate these associations. Full article

Introduction: Infantile Refsum disease (IRD) is considered one of the milder phenotypes within the Zellweger Spectrum Disorders (ZSDs), a group of peroxisomal biogenesis disorders characterized by a generalized impairment of peroxisomal function. Pathognomonic features of IRD are growth retardation, hearing and cognitive impairment, neuromuscular problems, and craniofacial anomalies. Due to the relatively short lifespan, severe dental anomalies have not been previously reported in association with this disorder. This case report describes a rare manifestation of multiple impacted teeth and near complete edentulism in an adult patient diagnosed with Infantile Refsum disease (IRD). Material and Methods: The patient, a 24-year-old female, presented with a skeletal Class III malocclusion, severe maxillary hypoplasia, and complete impaction of the permanent dentition. The diagnosis of Infantile Refsum disease (IRD) was genetically confirmed and was associated with both neurological and dermatological manifestations. Conclusions: This case underscores that severe disturbances in tooth eruption and impaction may represent underrecognized manifestations of metabolic and genetic disorders such as Infantile Refsum disease (IRD). Dental professionals should remain vigilant to the potential association between systemic conditions and delayed or failed tooth eruption, emphasizing the need for interdisciplinary management and further investigation. Full article

Background/Objectives: Despite decades of technological progress, the diagnosis of dental caries still depends largely on subjective, operator-dependent assessment, leading to inconsistent detection of early lesions and delayed intervention. Artificial intelligence (AI) has emerged as a transformative approach capable of standardizing diagnostic performance and, in some cases, surpassing human accuracy. This scoping review critically synthesizes the current evidence on AI for caries detection and examines its true translational readiness for clinical practice. Methods: A comprehensive literature search was conducted in PubMed, Scopus, and Web of Science (WoS), covering studies published from January 2019 to June 2024, in accordance with PRISMA-ScR guidelines. Eligible studies included original research evaluating the use of AI for dental caries detection, published in English or Spanish. Review articles, editorials, opinion papers, and studies unrelated to caries detection were excluded. Two reviewers independently screened, extracted, and charted data on imaging modality, sample characteristics, AI architecture, validation approach, and diagnostic performance metrics. Extracted data were summarized narratively and comparatively across studies using tabulated and graphical formats. Results: Thirty studies were included from an initial pool of 617 records. Most studies employed convolutional neural network (CNN)-based architectures and reported strong diagnostic performance, although these results come mainly from experimental settings and should be interpreted with caution. Bitewing radiography dominated the evidence base, reflecting technological maturity and greater reproducibility compared with other imaging modalities. Conclusions: Although the reported metrics are technically robust, the current evidence remains insufficient for real-world clinical adoption. Most models were trained on small, single-source datasets that do not reflect clinical diversity, and only a few underwent external or multicenter validation. Until these translational and methodological gaps are addressed, AI for caries detection should be regarded as promising yet not fully clinically reliable. By outlining these gaps and emerging opportunities, this review offers readers a concise overview of the current landscape and the key steps needed to advance AI toward meaningful clinical implementation. Full article

The interplay between the oral and gut microbiota and systemic health has garnered significant attention in recent years, particularly concerning hematological malignancies. Multiple myeloma and other hematological cancers are characterized by immune dysfunction, creating a bidirectional relationship with microbial communities. Dysbiosis, defined as an imbalance in microbial composition, may influence disease progression, treatment response, and overall prognosis. This narrative review is based on a non-systematic search of PubMed and Scopus (2010–2024) using terms related to oral microbiota, gut microbiota, dysbiosis, hematological malignancies, multiple myeloma, immune modulation, and treatment-related complications. Studies were selected for relevance to pathogenesis, immune regulation, clinical implications, and therapeutic interactions. As this is a narrative review, no quantitative synthesis or formal grading of evidence strength was performed; findings are therefore interpreted qualitatively based on the available literature. The role of microbial-derived metabolites, their effects on immune modulation, and their potential as biomarkers for disease and treatment outcomes have been explored. Specific attention is given to the implications of dysbiosis in chemotherapy-induced complications, such as mucositis and infections, and emerging therapeutic strategies, including probiotics, prebiotics, and fecal microbiota transplantation. Additionally, the influence of anticancer therapies on microbial ecosystems has been highlighted and the bidirectional impact of host–microbe interactions in shaping disease trajectory has been discussed. Understanding these complex interactions could lead to novel diagnostic and therapeutic approaches, ultimately improving patient outcomes. This review aims to provide clinicians and researchers with a comprehensive overview of current knowledge and future perspectives on the role of oral and gut microbiota in the context of hematological malignancies. Full article

Background/Objectives: Dentin hypersensitivity (DH) is associated with gingival recession and dentin exposure. Biomimetic hydroxyapatite (HAp) reduces DH by occluding dentinal tubules, with conventional toothpaste formulations showing benefits. High-density HAp mouthwashes may enhance bioavailability, but comparative evidence is scarce. This trial assessed the immediate desensitizing efficacy of a conventional HAp toothpaste and a high-density HAp mouthwash after professional oral hygiene. Methods: One hundred participants were randomized 1:1 to Biorepair^® (Coswell S.p.A., Funo, BO, Italy) Total Protection Toothpaste (Control) or Biorepair^® (Coswell S.p.A., Funo, BO, Italy) High-Density Mouthwash (Test). Assessments were performed at baseline (T0), post-debridement (T1), and after product use (T2). The primary endpoint was patient-level Schiff Air Index (SAI). Secondary endpoints included tooth-level SAI, Visual Analog Scale (VAS) scores, and gingival recession (GR). The trial was registered at ClinicalTrials.gov (NCT07057141) and followed CONSORT 2025 guidelines. Friedman and Dunn’s tests and regression models were applied. Results: Both groups showed significant reductions in hypersensitivity. Patient-level mean SAI decreased from 1.47 to 0.66 in the Control and from 1.48 to 0.45 in the Test group, while VAS declined from 3.66 to 1.57 (Control) and from 4.15 to 1.37 (Test). Post hoc analyses showed significant intragroup reductions between T0/T1 and T2 in both groups, with no significant differences between groups at any timepoint. GR remained stable across the study. Regression analyses identified follow-up time and GR as significant predictors, whereas treatment allocation was not, indicating that the acute advantage of the mouthwash at T2 did not persist once longitudinal trends were considered. Conclusions: Both HAp formulations effectively reduced dentin hypersensitivity 30 s after application. The high-density mouthwash exhibited slightly lower mean values at T2, although these differences were not statistically significant. Full article

Background/Purpose: The study aimed to analyze the dimensions and width-to-length ratios of the maxillary anterior teeth in young native Cambodian adults and to assess their relationship with the golden proportion, symmetry, and sexual dimorphism. Materials and Methods: Maxillary study casts of 193 eligible Cambodian subjects, aged 18 to 25 years, were retrospectively evaluated. The width and length of their maxillary anterior teeth were measured using a digital caliper. Descriptive statistics, independent-samples t-test at 95% confidence intervals, Kolmogorov-Smirnov, Shapiro-Wilk, and Kruskal-Wallis tests were performed to analyze the data. Results: There was a high level of similarity between first and second quadrant measurements. Females showed slightly higher standard deviations for central incisors and lateral incisors than males across most ratios, indicating more variability in the width-in-length ratios for females. Males exhibited significantly greater tooth dimensions than females. The following results showed statistical significance with p < 0.05 and 95% confidence intervals. The mean crown width of the central incisors was 8.16 mm in males (CI: 8.03–8.29) and 7.87 mm in females (CI: 7.78–7.96). For the lateral incisors, the mean crown width was 6.69 mm in males (CI: 6.53–6.85) and 7.64 mm in females (CI: 7.43–7.85). The width-to-length ratio of the central incisors was higher in females (mean = 0.88; CI: 0.86–0.91) compared with males (mean = 0.87; CI: 0.84–0.89). Overall, proportional relationships remained consistent across genders. The golden proportion guideline was not applicable, as observed ratios ranged from 0.90 to 1.67 (all below 1.618), and RED values exceeded 80%. The null hypothesis was rejected due to the significant gender differences found in tooth dimensions and width-to-length ratios. Conclusions: There was no significant difference in maxillary anterior tooth dimensions for the right and left sides among the Cambodian population. Males had statistically larger teeth than females. Width-to-length ratios were greater in females for central incisors; however, the proportional relationships between the genders remained relatively consistent. The golden proportion and RED proportions did not exist within this population. A smaller size characterizes Cambodian dentition compared to that of other ethnic groups. Finally, these results can serve as an indicator for planning customized esthetic treatment in Cambodians. Future studies with larger sample sizes are needed to ensure the representation of the whole Cambodian population. Full article