Journal of Functional Biomaterials

17 pages, 728 KiB

Open AccessSystematic Review

Accompanying Titanium Meshes and Titanium-Reinforced Membranes with Collagen Membranes in Vertical Alveolar Ridge Augmentations: A Systematic Review

by Amir-Ali Yousefi-Koma, Reza Amid, Anahita Moscowchi, Hanieh Nokhbatolfoghahaei and Mahdi Kadkhodazadeh

J. Funct. Biomater. 2025, 16(7), 246; https://doi.org/10.3390/jfb16070246 - 4 Jul 2025

Abstract

Background: Vertical ridge augmentations (VRAs), including guided bone regeneration (GBR) techniques, have been utilized in the reconstruction of deficient alveolar ridges for quite some time. GBR-based VRA procedures are technique-sensitive, operator-dependent, and often lead to complications detected during or after the treatment. The [...] Read more.

Background: Vertical ridge augmentations (VRAs), including guided bone regeneration (GBR) techniques, have been utilized in the reconstruction of deficient alveolar ridges for quite some time. GBR-based VRA procedures are technique-sensitive, operator-dependent, and often lead to complications detected during or after the treatment. The main objective of this systematic review was to include randomized and non-randomized human studies that investigated the regenerative outcome differences, as well as the incidence rates of healing and surgical complications of titanium meshes and/or titanium-reinforced membranes with and without collagen membranes utilized in GBR-based VRA. Methods: This systematic review has been prepared and organized according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) 2020 guidelines and is registered at PROSPERO (Registration ID: CRD420251002615). Medline via PubMed, Scopus, Web of Science, Embase, and the Cochrane Library were searched for eligible studies up to 5 June 2025. Randomized and non-randomized human clinical studies, except for case reports, focused on applying titanium meshes or titanium-reinforced membranes with or without collagen membranes in GBR-based VRA, were eligible. Results: A total of 119 patients from three human randomized clinical trials (RCTs) and one case series reported across nine articles were included. The addition of collagen membranes causes no significant differences in vertical bone gain or surgical/healing complication rates. Conclusions: The addition of collagen membranes on top of titanium meshes and titanium-reinforced membranes might not be necessary in GBR-based VRA. Further human RCTs are required to reach a reliable conclusion. Full article

(This article belongs to the Section Dental Biomaterials)

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13 pages, 9364 KiB

Open AccessArticle

Prevention of Tooth Discoloration Using Fluoride Varnish Immediately After Bleaching

by Ryotaro Yago, Chiharu Kawamoto, Rafiqul Islam, Hirofumi Kaneko, Monica Yamauti, Masayuki Otsuki, Hidehiko Sano and Atsushi Tomokiyo

J. Funct. Biomater. 2025, 16(7), 245; https://doi.org/10.3390/jfb16070245 - 3 Jul 2025

Abstract

Tooth bleaching is a widely used esthetic treatment; however, bleaching agents can temporarily alter the surface morphology of enamel, increasing surface roughness and porosity, which may lead to increased susceptibility to discoloration. This in vitro study investigated the effectiveness of fluoride varnish in [...] Read more.

Tooth bleaching is a widely used esthetic treatment; however, bleaching agents can temporarily alter the surface morphology of enamel, increasing surface roughness and porosity, which may lead to increased susceptibility to discoloration. This in vitro study investigated the effectiveness of fluoride varnish in preventing immediate discoloration of bovine incisors after bleaching. Specimens were bleached with 35% hydrogen peroxide and treated with either Clinpro White Varnish (CW) or Enamelast Fluoride Varnish (EN), whereas control specimens received no treatment after bleaching. The samples were immersed in coffee for 24 h, and the color difference (ΔE₀₀) was calculated using the CIEDE2000 formula. The surface morphology of enamel was examined using SEM. The fluoride varnish groups showed significantly lower color difference values than the control group (p < 0.05), with ΔE₀₀ reduced by approximately two-thirds in both the CW and EN groups. SEM observations showed that the enamel surfaces in the varnish-treated groups exhibited reduced surface irregularities compared to the untreated group, suggesting remineralization. These results suggest that the immediate application of fluoride varnish after bleaching can effectively reduce short-term discoloration by providing physical protection and promoting remineralization. Fluoride varnish may serve as a simple and effective strategy to maintain whitening outcomes and minimize early discoloration. Full article

(This article belongs to the Special Issue Active Biomedical Materials and Their Applications, 2nd Edition)

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19 pages, 523 KiB

Open AccessReview

Usage of Silver Nanoparticles in Orthodontic Bonding Reagents

by Janet Jisoo Lee, Meigan Niu, Zinah Shakir, Geelsu Hwang, Chun-Hsi Chung, Mark S. Wolff, Zhong Zheng and Chenshuang Li

J. Funct. Biomater. 2025, 16(7), 244; https://doi.org/10.3390/jfb16070244 - 3 Jul 2025

Abstract

Fixed orthodontic appliances, which are cemented to tooth surfaces, complicate the maintenance of oral hygiene and create a rough surface that is favorable for bacteria attachment. Additionally, the presence of orthodontic appliances may conceive a unique environment that interacts with cariogenic microorganisms, fostering [...] Read more.

Fixed orthodontic appliances, which are cemented to tooth surfaces, complicate the maintenance of oral hygiene and create a rough surface that is favorable for bacteria attachment. Additionally, the presence of orthodontic appliances may conceive a unique environment that interacts with cariogenic microorganisms, fostering a distinct microbial ecosystem compared to that of the patients without orthodontic appliances, thus increasing the vulnerability of tooth surfaces to demineralization and caries formation. Silver (Ag) has shown strong antimicrobial effects and has been extensively investigated in the medical field. Here, we aim to review the antibacterial properties and potential side effects of silver nanoparticles (AgNPs) when incorporated into orthodontic bonding reagents. This valuation could contribute to the development of novel bonding reagents designed to prevent the formation of white spot lesions and caries during orthodontic treatments. Full article

(This article belongs to the Special Issue Dental Biomaterials in Implantology and Orthodontics)

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31 pages, 5332 KiB

Open AccessReview

Photothermal Release by Melanin-like Nanoparticles: Biomedical Applications

by Arianna Menichetti, Silvia Vicenzi, Agata Pane, Dario Mordini, Fabrizio Mancin and Marco Montalti

J. Funct. Biomater. 2025, 16(7), 243; https://doi.org/10.3390/jfb16070243 - 2 Jul 2025

Abstract

Melanin-like nanoparticles (NPs) exhibit a remarkable ability to absorb light across a wide range of wavelengths, from the ultraviolet (UV) to the near-infrared (NIR) spectrum. This characteristic enables them to serve as effective photothermal agents (PTAs). Upon irradiation, especially within the NIR window, [...] Read more.

Melanin-like nanoparticles (NPs) exhibit a remarkable ability to absorb light across a wide range of wavelengths, from the ultraviolet (UV) to the near-infrared (NIR) spectrum. This characteristic enables them to serve as effective photothermal agents (PTAs). Upon irradiation, especially within the NIR window, a region where biological tissues are highly transparent, these NPs efficiently convert light energy into heat. This phenomenon, known as the photothermal effect, leads to localized temperature increases. The resulting heat can be strategically employed to induce selective cell death in photothermal therapy (PTT) or to enhance the release of therapeutic agents directly from the NPs. The inherent versatility of melanin-like NPs, stemming from their synthesis methods and the presence of various functional groups, allows for straightforward loading with drugs or other bioactive molecules. Consequently, they are attractive tools for photothermally activated release. This review paper thoroughly examines and critically discusses the latest applications of melanin-like NPs in photothermally controlled release. We dedicate a specific section to general mechanisms and approaches, and this paper concludes with an analysis of critical challenges and prospective future developments. Full article

(This article belongs to the Special Issue Nanomaterials for Drug Targeting and Drug Delivery (2nd Edition))

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15 pages, 1530 KiB

Open AccessArticle

One- and Two-Year Efficacy of Resin Infiltration and Remineralization for the Treatment of Initial Proximal Caries

by Veselina Todorova and Ivan Filipov

J. Funct. Biomater. 2025, 16(7), 242; https://doi.org/10.3390/jfb16070242 - 1 Jul 2025

Abstract

Proximal caries presents diagnostic and therapeutic challenges. Recent understanding of the etiology and pathology of dental caries has led to the adoption of non-invasive and/or minimally invasive approaches in the early stages of caries lesions. This clinical study aimed to compare the efficacy [...] Read more.

Proximal caries presents diagnostic and therapeutic challenges. Recent understanding of the etiology and pathology of dental caries has led to the adoption of non-invasive and/or minimally invasive approaches in the early stages of caries lesions. This clinical study aimed to compare the efficacy of resin infiltration and remineralization in the treatment of initial proximal caries lesions over a 1- and 2-year follow-up period. The study involved 47 patients aged between 18 and 38 years. Patients were clinically examined and underwent bitewing radiography to detect at least three initial proximal caries lesions. Each detected lesion (180 in total) was randomly assigned to one of three treatment groups: (1) resin infiltration with Icon Proximal Infiltrant (DMG); (2) remineralization with Clinpro White Varnish (3M); and (3) a control group receiving no treatment. One year after treatment, caries progression was found in 30 lesions (16.6%) with the following distribution across the three treatment groups: 2/60 (3%) in the infiltration group; 11/60 (18%) in the remineralization group; 17/60 (28.30%) in the no treatment control group with a significant statistical difference between the groups (p = 0.001). In terms of lesion depth, 12 (11%) out of 106 E2 lesions progressed and 18 out of 74 (24%) D1 lesions progressed, with a significant difference (p = 0.037). Two years after treatment, five new lesions were found to have progressed (one E2 and four D1), distributed as follows: 0% in the infiltration group, 3.6% in the remineralization group, and 5% in the control group. In conclusion, resin infiltration exhibited the lowest percentage of progressed lesions and could be considered a reliable, non-invasive treatment for initial proximal caries. Full article

(This article belongs to the Special Issue The Development and Future of Dental Implants)

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18 pages, 2460 KiB

Open AccessArticle

Extracellular Synthesis of Bioactive Silver Nanoparticles Using Brevibacillus sp. MAHUQ-41 and Their Potential Application Against Drug-Resistant Bacterial Pathogens Listeria monocytogenes and Yersinia enterocolitica

by Md. Amdadul Huq

J. Funct. Biomater. 2025, 16(7), 241; https://doi.org/10.3390/jfb16070241 - 30 Jun 2025

Abstract

The purpose of current study was the green synthesis of bioactive silver nanoparticles (AgNPs) using Brevibacillus sp. MAHUQ-41 and the exploration of their role in controlling drug-resistant bacterial pathogens Listeria monocytogenes and Yersinia enterocolitica. The culture supernatant of strain MAHUQ-41 was employed [...] Read more.

The purpose of current study was the green synthesis of bioactive silver nanoparticles (AgNPs) using Brevibacillus sp. MAHUQ-41 and the exploration of their role in controlling drug-resistant bacterial pathogens Listeria monocytogenes and Yersinia enterocolitica. The culture supernatant of strain MAHUQ-41 was employed for a simple and eco-friendly synthesis of biofunctional silver nanoparticles (AgNPs). The resulting nanoparticles were analyzed using several techniques, including UV–Visible spectroscopy, XRD, FE-TEM, FTIR, and DLS. The UV–Vis spectral analysis of the AgNPs synthesized via Brevibacillus sp. MAHUQ-41 revealed a prominent absorption peak at 400 nm. FE-TEM results confirmed spherical-shaped 15–60 nm sized nanoparticles. XRD results indicated that the synthesized AgNPs were crystalline in nature. The FTIR spectrum determined various functional groups on the surface of synthesized nanoparticles. Potent antibacterial properties were observed in green-synthesized AgNPs against tested pathogens. The MIC value of extracellular synthesized AgNPs for both pathogenic bacteria was 6.2 µg/mL, and the MBCs were 25.0 µg/mL and 12.5 µg/mL for L. monocytogenes and Y. enterocolitica, respectively. Treatment by synthesized AgNPs resulted in morphological alterations and structural damages in both L. monocytogenes and Y. enterocolitica. These alterations can interfere with regular cellular activities, potentially resulting in cell death. This study is the first to report the antimicrobial properties of silver nanoparticles synthesized using Brevibacillus sp. MAHUQ-41. The findings obtained in the present study supported the role of Brevibacillus sp. MAHUQ-41-mediated synthesized AgNPs in controlling drug-resistant bacterial pathogens L. monocytogenes and Y. enterocolitica. Full article

(This article belongs to the Special Issue Antimicrobial Biomaterials for Medical Applications)

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44 pages, 2343 KiB

Open AccessReview

Head and Neck 3D Bioprinting—A Review on Recent Advancements in Soft Tissue 3D Bioprinting and Medical Applications

by Iosif-Aliodor Timofticiuc, Ana Caruntu, Christiana Diana Maria Dragosloveanu, Andreea-Elena Scheau, Ioana Anca Badarau, Argyrios Periferakis, Serban Dragosloveanu, Andreea Cristiana Didilescu, Constantin Caruntu and Cristian Scheau

J. Funct. Biomater. 2025, 16(7), 240; https://doi.org/10.3390/jfb16070240 - 30 Jun 2025

Abstract

Recent developments in 3D bioprinting offer innovative alternative solutions to classical treatments for head and neck defects. Soft tissues in an anatomical area as diverse in composition as the head and neck are complex in terms of structure and function. Understanding how cellular [...] Read more.

Recent developments in 3D bioprinting offer innovative alternative solutions to classical treatments for head and neck defects. Soft tissues in an anatomical area as diverse in composition as the head and neck are complex in terms of structure and function. Understanding how cellular interaction underlies functionality has led to the development of bioinks capable of mimicking the natural morphology and roles of different human parts. Moreover, from the multitude of recently developed materials, there are now many options for building scaffolds that potentiate the activity of these cells. The fidelity and accuracy of the utilized techniques ensure maximum precision in terms of model construction. Emerging technologies will allow for improved control of the scaffold, facilitating optimal results in the treatment of various pathologies, without concerns about the availability of donors, immunological response, or any other side effects that traditional treatments withhold. This paper explores the current landscape of bioprinted scaffolds and their applications in the head and neck region, with a focus on the properties and use of natural and synthetic bioinks in the attempt to replicate the biomechanical features of native tissues. Customization capabilities that support anatomical precision and biofunctionality are also addressed. Moreover, regulatory requirements, as well as current challenges related to biocompatibility, immune response, and vascularization, are critically discussed in order to provide a comprehensive overview of the pathway to clinical application. Full article

(This article belongs to the Special Issue The 15th Anniversary of JFB—Functional Biomaterials: Bioactive Properties and Medical Applications)

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1 pages, 134 KiB

Open AccessCorrection

Correction: Seifalian et al. A Novel Graphene-Based Nanomaterial for the Development of a Pelvic Implant to Treat Pelvic Organ Prolapse. J. Funct. Biomater. 2024, 15, 351

by Amelia Seifalian, Alex Digesu and Vik Khullar

J. Funct. Biomater. 2025, 16(7), 239; https://doi.org/10.3390/jfb16070239 - 30 Jun 2025

Abstract

In the original publication [...] Full article

(This article belongs to the Section Biomaterials and Devices for Healthcare Applications)

30 pages, 742 KiB

Open AccessReview

Biomimetic Three-Dimensional (3D) Scaffolds from Sustainable Biomaterials: Innovative Green Medicine Approach to Bone Regeneration

by Yashaswini Premjit, Merin Lawrence, Abhishek Goyal, Célia Ferreira, Elena A. Jones and Payal Ganguly

J. Funct. Biomater. 2025, 16(7), 238; https://doi.org/10.3390/jfb16070238 - 29 Jun 2025

Abstract

Bone repair and regeneration following an injury still present challenges worldwide. Three-dimensional (3D) scaffolds made from various materials are used for bone tissue engineering (BTE) applications. Polymers, minerals and nanotechnology are now being used in combination to achieve specific goals for BTE, including [...] Read more.

Bone repair and regeneration following an injury still present challenges worldwide. Three-dimensional (3D) scaffolds made from various materials are used for bone tissue engineering (BTE) applications. Polymers, minerals and nanotechnology are now being used in combination to achieve specific goals for BTE, including the delivery of antimicrobials through the scaffolds to prevent post-surgical infection. While several materials are utilised for BTE, natural polymers present a unique set of materials that can be manipulated to formulate scaffolds for BTE applications. They have been found to demonstrate higher biocompatibility, biodegradability and lower toxicity. Some even naturally mimic the bone microarchitecture, providing inherent structural support for BTE. Natural polymers may be simply classified as those from plant and animal sources. From both sources, there are different types of proteins, polysaccharides and other specialised materials that are already in use for research in BTE. Interestingly, these have the potential to revolutionise the field of BTE with a sustainable approach. In this review, we first discuss the different natural polymers used in BTE from plant sources, followed by animal sources. We then explore novel materials that are aimed at sustainable approaches, focusing on innovation from the last decade. In these sections, we outline studies of these materials with different types of bone cells, including bone marrow mesenchymal stromal cells (MSCs), which are the progenitors of bone. We finally outline the limitations, conclusions and future directions from our perspective in this dynamic field of polymers in BTE. With this review, we hope to bring together the updated existing knowledge and the potential future of innovation and sustainability in natural polymers for biomimetic BTE applications for fellow scientists, researchers and surgeons in the field. Full article

(This article belongs to the Special Issue Novel Biomaterials for Tissue Engineering)

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26 pages, 3738 KiB

Open AccessArticle

Morphometric, Biomechanical and Macromolecular Performances of β-TCP Macro/Micro-Porous Lattice Scaffolds Fabricated via Lithography-Based Ceramic Manufacturing for Jawbone Engineering

by Carlo Mangano, Nicole Riberti, Giulia Orilisi, Simona Tecco, Michele Furlani, Christian Giommi, Paolo Mengucci, Elisabetta Giorgini and Alessandra Giuliani

J. Funct. Biomater. 2025, 16(7), 237; https://doi.org/10.3390/jfb16070237 - 28 Jun 2025

Abstract

Effective bone tissue regeneration remains pivotal in implant dentistry, particularly for edentulous patients with compromised alveolar bone due to atrophy and sinus pneumatization. Biomaterials are essential for promoting regenerative processes by supporting cellular recruitment, vascularization, and osteogenesis. This study presents the development and [...] Read more.

Effective bone tissue regeneration remains pivotal in implant dentistry, particularly for edentulous patients with compromised alveolar bone due to atrophy and sinus pneumatization. Biomaterials are essential for promoting regenerative processes by supporting cellular recruitment, vascularization, and osteogenesis. This study presents the development and characterization of a novel lithography-printed ceramic β-TCP scaffold, with a macro/micro-porous lattice, engineered to optimize osteoconduction and mechanical stability. Morphological, structural, and biomechanical assessments confirmed a reproducible microarchitecture with suitable porosity and load-bearing capacity. The scaffold was also employed for maxillary sinus augmentation, with postoperative evaluation using micro computed tomography, synchrotron imaging, histology, and Fourier Transform Infrared Imaging analysis, demonstrating active bone regeneration, scaffold resorption, and formation of mineralized tissue. Advanced imaging supported by deep learning tools revealed a well-organized osteocyte network and high-quality bone, underscoring the scaffold’s biocompatibility and osteoconductive efficacy. These findings support the application of these 3D-printed β-TCP scaffolds in regenerative dental medicine, facilitating tissue regeneration in complex jawbone deficiencies. Full article

(This article belongs to the Special Issue Functional Biomaterial for Bone Regeneration)

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21 pages, 2737 KiB

Open AccessArticle

Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions

by Jiale Wang, Jiawei Guan, Xingyu Ma, Dongyang Zhao, Yongqiang Han, Dongdong Guo, Jialin Bai, Zisheng Guo and Xiaojun Zhang

J. Funct. Biomater. 2025, 16(7), 236; https://doi.org/10.3390/jfb16070236 - 27 Jun 2025

Abstract

The effective healing of chronic wounds requires balancing antimicrobial activity with tissue regeneration. In this study, we developed a novel, eco-friendly synthesis method using Artemisia argyi extract to produce silver nanoparticles (AgNPs), addressing toxicity concerns associated with conventional chemical synthesis methods. Through optimization [...] Read more.

The effective healing of chronic wounds requires balancing antimicrobial activity with tissue regeneration. In this study, we developed a novel, eco-friendly synthesis method using Artemisia argyi extract to produce silver nanoparticles (AgNPs), addressing toxicity concerns associated with conventional chemical synthesis methods. Through optimization of multiple synthesis parameters, monodisperse spherical AgNPs with an average diameter of 6.76 ± 0.27 nm were successfully obtained. Plant-derived compounds from Artemisia argyi extract acted as efficient mediators for both reduction and stabilization, yielding nanoparticles with high crystallinity. The synthesized AgNPs exhibited potent antibacterial activity against both Gram-negative and Gram-positive bacteria, with minimum inhibitory concentrations of 8 μg/mL against Escherichia coli and 32 μg/mL against Staphylococcus aureus, while maintaining high biocompatibility with L929 fibroblasts at concentrations ≤ 8 μg/mL. When integrated into polylactic acid/collagen type I (PLA/Col1) nanofibrous matrices, the optimized 0.03% AgNPs/PLA/Col1 dressing significantly accelerated wound healing in a diabetic rat model, achieving 94.62 ± 2.64% wound closure by day 14 compared to 65.81 ± 1.80% observed in untreated controls. Histological analyses revealed a dual-functional mechanism wherein controlled silver ion release provided sustained antibacterial protection, while concurrently promoting tissue regeneration characterized by enhanced collagen deposition, reduced inflammation, and increased neovascularization. This innovative approach effectively addresses critical challenges in diabetic wound care by providing simultaneous antimicrobial and regenerative functions within a single biomaterial platform. Full article

(This article belongs to the Section Biomaterials for Tissue Engineering and Regenerative Medicine)

14 pages, 2179 KiB

Open AccessArticle

One-Pot Anodic Electrodeposition of Dual-Cation-Crosslinked Sodium Alginate/Carboxymethyl Chitosan Interpenetrating Hydrogel with Vessel-Mimetic Heterostructures

by Xuli Li, Yuqing Qu, Yong Zhang, Pei Chen, Siyu Ding, Miaomiao Nie, Kun Yan and Shefeng Li

J. Funct. Biomater. 2025, 16(7), 235; https://doi.org/10.3390/jfb16070235 - 26 Jun 2025

Abstract

This study develops a one-pot anodic templating electrodeposition strategy using dual-cation-crosslinking and interpenetrating networks, coupled with pulsed electrical signals, to fabricate a vessel-mimetic multilayered tubular hydrogel. Typically, the anodic electrodeposition is performed in a mixture of sodium alginate (SA) and carboxymethyl chitosan (CMC), [...] Read more.

This study develops a one-pot anodic templating electrodeposition strategy using dual-cation-crosslinking and interpenetrating networks, coupled with pulsed electrical signals, to fabricate a vessel-mimetic multilayered tubular hydrogel. Typically, the anodic electrodeposition is performed in a mixture of sodium alginate (SA) and carboxymethyl chitosan (CMC), with the ethylenediaminetetraacetic acid calcium disodium salt hydrate (EDTA·Na₂Ca) incorporated to provide a secondary ionic crosslinker (i.e., Ca²⁺) and modulate the cascade reaction diffusion process. The copper wire electrodes serve as templates for electrochemical oxidation and enable a copper ion (i.e., Cu²⁺)-induced tubular hydrogel coating formation, while pulsed electric fields regulate layer-by-layer deposition. The dual-cation-crosslinked interpenetrating hydrogels (CMC/SA-Cu/Ca) exhibit rapid growth rates and tailored mechanical strength, along with excellent antibacterial performance. By integrating the unique pulsed electro-fabrication with biomimetic self-assembly, this study addresses challenges in vessel-mimicking structural complexity and mechanical compatibility. The approach enables scalable production of customizable multilayered hydrogels for artificial vessel grafts, smart wound dressings, and bioengineered organ interfaces, demonstrating broad biomedical potential. Full article

(This article belongs to the Special Issue Advances in Multifunctional Hydrogels for Biomedical Application (2nd Edition))

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14 pages, 3988 KiB

Open AccessArticle

Evaluation of the Bioinductive Effects of a Novel Antibiotic Eluting Cardiac Implantable Electronic Device Envelope

by Sun Woo Kim, Nathan W. Fedak, Eleanor Love, Alexander Tam, Ali Fatehi Hassanabad, Jeannine Turnbull, Guoqi Teng, Darrell Belke, Justin Deniset and Paul W. M. Fedak

J. Funct. Biomater. 2025, 16(7), 234; https://doi.org/10.3390/jfb16070234 - 25 Jun 2025

Abstract

Background: Subcutaneous pocket infection is a common morbidity associated with the integration of cardiac implantable electronic devices (CIEDs). A new antibiotic-eluting CIED bioenvelope has been developed as a prophylactic measure to mitigate infection and skin erosion caused by device migration. This study investigated [...] Read more.

Background: Subcutaneous pocket infection is a common morbidity associated with the integration of cardiac implantable electronic devices (CIEDs). A new antibiotic-eluting CIED bioenvelope has been developed as a prophylactic measure to mitigate infection and skin erosion caused by device migration. This study investigated the envelope’s regulatory properties in scar formation and vascularization. Methods: Fibroblasts were seeded on either plastic (n = 6) or small intestine submucosal extracellular matrix (SIS-ECM) (n = 6) for 24 h. The culture media were analyzed for proangiogenic and proinflammatory proteins with multiplex. Sham (n = 8) or SIS-ECM (n = 8) was randomly implanted into the dorsal subcutaneous pocket of mice. The implants were excised on day 7, cultured for 24 h, and the media analyzed. Rabbit models were implanted with either synthetic polymer HDPE (n = 12) or SIS-ECM (n = 11). The treatments were excised at weeks 2, 10, and 26 and then stained for analysis. Results: SIS-ECM significantly increased the fibroblasts’ paracrine release of proangiogenic and proinflammatory factors like VEGF-A (p < 0.05) and IL-6 (p < 0.05) compared with plastic. The murine tissue interacting with SIS-ECM released significantly more angiogenic proteins like VEGF-A (p < 0.05) than the sham. The histology analysis of rabbit subcutaneous tissue revealed a decreasing level of inflammation and fibrosis over time with SIS-ECM. Conclusions: The CIED bioenvelope elicited proangiogenic paracrine signaling and reduced fibrotic response in fibroblasts and animal models. Clinical translation of the CIED bioenvelope as an adjunct to regular prophylactic practice may be warranted in the future. Full article

(This article belongs to the Section Biomaterials for Tissue Engineering and Regenerative Medicine)

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17 pages, 2746 KiB

Open AccessArticle

Histological Analysis of Sticky Tooth and Sticky Bone

by Robert Dłucik, Marcel Firlej, Katarzyna Bogus, Daniel Dłucik and Bogusława Orzechowska-Wylęgała

J. Funct. Biomater. 2025, 16(7), 233; https://doi.org/10.3390/jfb16070233 - 25 Jun 2025

Abstract

Objective: This study aimed to compare the efficacy of Sticky Tooth (ST) derived from ground teeth and Sticky Bone (SB) based on equine bone and human allograft in maxillary bone defect regeneration through histological examination. Materials and Methods: Forty patients underwent maxillary alveolar [...] Read more.

Objective: This study aimed to compare the efficacy of Sticky Tooth (ST) derived from ground teeth and Sticky Bone (SB) based on equine bone and human allograft in maxillary bone defect regeneration through histological examination. Materials and Methods: Forty patients underwent maxillary alveolar ridge regeneration using four different biomaterials: Sticky Tooth processed with the BonMaker device (n = 10), Sticky Tooth prepared with the Smart Dentin Grinder (n = 10) Sticky Bone derived from an equine xenograft (n = 10), and Sticky Bone derived from human allografts (n = 10). CBCT imaging was performed preoperatively, post-regeneration, and during follow-up. Histological and quantitative statistical evaluation was conducted on biopsy samples obtained four months post-regeneration at the time of implant placement. Results: Successful alveolar ridge regeneration was achieved in all 40 patients. Histological analyses confirmed good integration between the biomaterials and bone tissue without signs of inflammation. Conclusion: Histological comparisons demonstrated that both ST and SB are effective biomaterials for bone regeneration. However, ST exhibited a faster bone healing process compared to xenograft and allograft SB. Full article

(This article belongs to the Section Bone Biomaterials)

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18 pages, 683 KiB

Open AccessReview

Next-Generation Biomaterials for Load-Bearing Tissue Interfaces: Sensor-Integrated Scaffolds and Mechanoadaptive Constructs for Skeletal Regeneration

by Rahul Kumar, Kyle Sporn, Pranay Prabhakar, Phani Paladugu, Akshay Khanna, Alex Ngo, Chirag Gowda, Ethan Waisberg, Ram Jagadeesan, Nasif Zaman and Alireza Tavakkoli

J. Funct. Biomater. 2025, 16(7), 232; https://doi.org/10.3390/jfb16070232 - 23 Jun 2025

Abstract

Advancements in load-bearing tissue repair increasingly demand biomaterials that not only support structural integrity but also interact dynamically with the physiological environment. This review examines the latest progress in smart biomaterials designed for skeletal reconstruction, with emphasis on mechanoresponsive scaffolds, bioactive composites, and [...] Read more.

Advancements in load-bearing tissue repair increasingly demand biomaterials that not only support structural integrity but also interact dynamically with the physiological environment. This review examines the latest progress in smart biomaterials designed for skeletal reconstruction, with emphasis on mechanoresponsive scaffolds, bioactive composites, and integrated microsensors for real-time monitoring. We explore material formulations that enhance osseointegration, resist micromotion-induced loosening, and modulate inflammatory responses at the bone–implant interface. Additionally, we assess novel fabrication methods—such as additive manufacturing and gradient-based material deposition—for tailoring stiffness, porosity, and degradation profiles to match host biomechanics. Special attention is given to sensor-augmented platforms capable of detecting mechanical strain, biofilm formation, and early-stage implant failure. Together, these technologies promise a new class of bioresponsive, diagnostic-capable constructs that extend beyond static support to become active agents in regenerative healing and post-operative monitoring. This multidisciplinary review integrates insights from materials science, mechanobiology, and device engineering to inform the future of implantable systems in skeletal tissue repair. Full article

(This article belongs to the Special Issue Materials and Techniques for Bone Tissue Engineering: From Scaffolds to Complex Matrices)

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25 pages, 3895 KiB

Open AccessReview

The Biomedical Limitations of Magnetic Nanoparticles and a Biocompatible Alternative in the Form of Magnetotactic Bacteria

by Natalia L. Paul, Rahela Carpa, Rodica Elena Ionescu and Catalin Ovidiu Popa

J. Funct. Biomater. 2025, 16(7), 231; https://doi.org/10.3390/jfb16070231 - 23 Jun 2025

Abstract

Nanotechnology has an increasing impact and a great potential in various biological and medical applications. Magnetic nanoparticles (MNPs) stand out for their unique properties, a reason why they have a varied spectrum of applicability in different sectors of activity; in this paper we [...] Read more.

Nanotechnology has an increasing impact and a great potential in various biological and medical applications. Magnetic nanoparticles (MNPs) stand out for their unique properties, a reason why they have a varied spectrum of applicability in different sectors of activity; in this paper we focus on the medical field. Magnetotactic bacteria (MTB) are a group of Gram-negative prokaryotes that migrate in one direction or another under the influence of an external magnetic field and are a category of microorganisms that constitutively perform the biomineralization of magnetic nanoparticles in the cytoplasm. This review focuses on the general and particular characteristics of magnetotactic bacteria in close correlation with their utility in the medical field, starting with the medical applications of magnetic nanoparticles and arriving at the potential role in nanomedicine of MNPs extracted from MTB. Full article

(This article belongs to the Special Issue Biomaterials: Functionalization, Diagnostics, and Modeling for Advanced Therapeutic and Regenerative Applications)

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17 pages, 2527 KiB

Open AccessArticle

The Impact of As-Built Surface Characteristics of Selective-Laser-Melted Ti-6Al-4V on Early Osteoblastic Response for Potential Dental Applications

by Muhammad Hassan Razzaq, Olugbenga Ayeni, Selin Köklü, Kagan Berk, Muhammad Usama Zaheer, Tim Tjardts, Franz Faupel, Salih Veziroglu, Yogendra Kumar Mishra, Mehmet Fatih Aycan, O. Cenk Aktas, Tayebeh Ameri and Sinan Sen

J. Funct. Biomater. 2025, 16(7), 230; https://doi.org/10.3390/jfb16070230 - 23 Jun 2025

Abstract

This study investigates the potential of Selective Laser Melting (SLM) to tailor the surface characteristics of Ti6Al4V directly during fabrication, eliminating the need for post-processing treatments potentially for dental implants. By adjusting the Volumetric Energy Density (VED) through controlled variations in the laser [...] Read more.

This study investigates the potential of Selective Laser Melting (SLM) to tailor the surface characteristics of Ti6Al4V directly during fabrication, eliminating the need for post-processing treatments potentially for dental implants. By adjusting the Volumetric Energy Density (VED) through controlled variations in the laser scanning speed, we achieved customized surface textures at both the micro- and nanoscale levels. SLM samples fabricated at moderate VED levels (50–100 W·mm³/s) exhibited optimized dual-scale surface roughness—a macro-roughness of up to 25.5–27.6 µm and micro-roughness of as low as 58.8–64.2 nm—resulting in significantly enhanced hydrophilicity, with water contact angles (WCAs) decreasing to ~62°, compared to ~80° on a standard grade 5 machined Ti6Al4V plate. The XPS analysis revealed that the surface oxygen content remains relatively stable at low VED values, with no significant increase. The surface topography plays a significant role in influencing the WCA, particularly when the VED values are low (below 200 W·mm³/s) during SLM, indicating the dominant effect of surface morphology over chemistry in these conditions. Biological assays using osteoblast-like MG-63 cells demonstrated that these as-built SLM surfaces supported a 1.5-fold-higher proliferation and improved cytoskeletal organization relative to the control, confirming the enhanced early cellular responses. These results highlight the capability of SLM to engineer bioactive implant surfaces through process-controlled morphology and chemistry, presenting a promising strategy for the next generation of dental implants suitable for immediate placement and osseointegration. Full article

(This article belongs to the Section Dental Biomaterials)

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15 pages, 2478 KiB

Open AccessArticle

Fe(III)-Based Nanomicelles for Magnetic Resonance Imaging of Colorectal Cancer with Hepatic Metastasis

by Tianlun Shen, Kaiwei Lv, Zhenyan Chen, Songyi Xu, Guangyao Li, Guocan Han, Yì Xiáng J. Wáng, Jun Ling and Jihong Sun

J. Funct. Biomater. 2025, 16(7), 229; https://doi.org/10.3390/jfb16070229 - 20 Jun 2025

Abstract

Colorectal cancer (CRC) is a leading global malignancy with a poor prognosis in advanced stages. Early and accurate diagnosis remains challenging due to the overlapping of clinical manifestations between early-stage CRC and inflammatory bowel diseases. Although dynamic contrast-enhanced MRI (DCE-MRI) is a critical [...] Read more.

Colorectal cancer (CRC) is a leading global malignancy with a poor prognosis in advanced stages. Early and accurate diagnosis remains challenging due to the overlapping of clinical manifestations between early-stage CRC and inflammatory bowel diseases. Although dynamic contrast-enhanced MRI (DCE-MRI) is a critical imaging modality for the diagnosis of CRC and colorectal cancer liver metastasis (CRLM), conventional gadolinium-based contrast agents (GBCAs) have the limitations of rapid clearance and potential toxicity risks. In this study, we report a gadolinium-free T1-weighted nanocontrast agent based on Fe(III)-coordinated poly(α-amino acid)s (Fe@POS) nanomicelles. Fe@POS nanomicelles exhibit a high longitudinal relaxivity (r₁ = 5.56 mM⁻¹s⁻¹) and prolonged blood circulation time with selective CRC tumor accumulation via enhanced permeability and retention (EPR) effect. In vivo MRI studies revealed long-period MRI of CRC. In CRLM lesions, normal hepatic tissue demonstrates greater Fe@POS uptake compared to tumor tissue, which enables clear delineation of tumor margins on MRI. Histological and biochemical analysis confirmed the biocompatibility of Fe@POS nanomicelles, with no acute toxicity observed, highlighting their potential as alternatives to GBCAs for clinical diagnostic applications. Full article

(This article belongs to the Section Biomaterials and Devices for Healthcare Applications)

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16 pages, 1024 KiB

Open AccessSystematic Review

Potential Applications of PRP-Enhanced Polybutylene Succinate Graft as Vascular Access for Chemotherapy in Oncological Patients: A Systematic Review

by Andrea Gottardo, Giulia Bonventre, Tancredi Didier Bazan Russo, Pietro Zanatta, Giulia Lo Monte, Valerio Gristina, Antonio Galvano, Antonio Russo and Attilio Ignazio Lo Monte

J. Funct. Biomater. 2025, 16(6), 228; https://doi.org/10.3390/jfb16060228 - 19 Jun 2025

Abstract

This systematic review aimed to evaluate the potential of combining platelet-rich plasma (PRP) and polybutylene succinate (PBS) for the development of vascular grafts in patients undergoing chemotherapy. Relevant articles published in English or Italian were selected through a comprehensive search of MEDLINE (via [...] Read more.

This systematic review aimed to evaluate the potential of combining platelet-rich plasma (PRP) and polybutylene succinate (PBS) for the development of vascular grafts in patients undergoing chemotherapy. Relevant articles published in English or Italian were selected through a comprehensive search of MEDLINE (via PubMed) and the Cochrane Library. A total of ten screened articles and two additional relevant studies were included. The synthesis of results was conducted using digital tools, thoroughly reviewed by the authors. The quality assessment of the included studies revealed a medium-to-high risk of bias, with frequent limitations such as small sample sizes, experimental designs, and overall moderate to low methodological quality. Despite the heterogeneity of the findings, the available evidence suggests that radiocephalic graft placement and the use of PBS as a scaffold material, in combination with the growth factors contained in PRP, may improve clinical outcomes and reduce complications related to arteriovenous graft implantation. While promising, the current literature on this topic remains scarce and fragmented, underscoring the need for additional preclinical and clinical research. The proposed approach appears to hold potential for improving vascular access in oncology, but further in vivo validation is essential. This study received no external funding. Registration: PROSPERO ID CRD42025646724. Full article

(This article belongs to the Section Biomaterials for Cancer Therapies)

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Journal of Functional Biomaterials

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