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Open AccessArticle

Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs

1
Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana
2
Department of Anatomy, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana
3
Department of Physiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra 00233, Ghana
4
Central Laboratory Services, Korle-Bu Teaching Hospital, Korle-Bu, Accra 00233, Ghana
5
Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra 00233, Ghana
*
Author to whom correspondence should be addressed.
Med. Sci. 2019, 7(4), 64; https://doi.org/10.3390/medsci7040064
Received: 21 March 2019 / Revised: 21 March 2019 / Accepted: 10 April 2019 / Published: 23 April 2019
(This article belongs to the Section Immunology and Infectious Diseases)
This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disease pathology, diagnosis, transmission, and drug development research, it is essential to be able to easily distinguish live eggs from dead ones. Staining is considered a way of enhancing the identification of live and dead eggs. Urine samples from school children were examined for the presence of S. haematobium eggs. Vital and fluorescent dyes were used to stain the samples that contained S. haematobium eggs, after which they were observed using light and fluorescent microscopes, respectively. The Hoechst 33258 provided a good staining outcome for differentiation between live and dead eggs, followed by 0.4% Trypan blue. Regarding the 1% neutral red stain, even though it provided some evidence of which egg was alive or dead, the distinction was not very clear; therefore, it could be useful when used in combination with other stains for egg viability determination. The benefits of this study will include assessing the effect of drugs on S. haematobium eggs in Schistosomiasis research. View Full-Text
Keywords: Hoechst 33258; schistosomiasis; Schistosoma haematobium; staining; viability; vital stains Hoechst 33258; schistosomiasis; Schistosoma haematobium; staining; viability; vital stains
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Forson, P.O.; Tetteh-Quarcoo, P.B.; Ahenkorah, J.; Aryee, R.; Okine, E.N.; Afutu, E.; Djameh, G.I.; Agyapong, J.; Anang, A.K.; Ayeh-Kumi, P.F. Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs. Med. Sci. 2019, 7, 64.

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