The current status of microbiological testing methods for the determination of viable bacteria in complex sample matrices, such as food samples, is the focus of this review. Established methods for the enumeration of microorganisms, particularly, the...
Compartmentalization of high-energy phosphate carriers between intracellular micro-compartments is a phenomenon that ensures efficient energy use. To connect these sites, creatine kinase (CK) and adenylate kinase (AK) energy-transfer networks, which...
MPP+ is the active metabolite of MPTP, a molecule structurally similar to the herbicide Paraquat, known to injure the dopaminergic neurons of the nigrostriatal system in Parkinson’s disease models. Within the cells, MPP+ accumulates in mitochon...
Submersion in the anaesthetic MS-222 is a well-established and effective method used during the euthanasia of fish, but the consequences of treatment with this anaesthetic for mitochondrial respiration are yet to be established. This is important to...
Composting is considered an option for the disposal of organic waste; however, the development of portable and low-cost systems for its monitoring is of high interest. Therefore, in this study, respirometric microsystems were designed and tested incl...
Inefficient management of dissolved oxygen (DO) in intensive aquaculture systems limits fish welfare and productivity by creating oxygen-deficient zones and promoting hydrodynamic conditions that hinder their dispersion. Because water movement direct...
The popular method of micro-respirometry (μR) for measuring total viable (aerobic) count (TVC) utilises luminescence-based O2 sensors that are difficult to fabricate and therefore expensive. A simple method is described for making inexpensive, ink...
Amyotrophic lateral sclerosis is a severe neurodegenerative disease whose exact cause is still unclear. Currently, research attention is turning to the mitochondrion as a critical organelle of energy metabolism. Current knowledge is sufficient to con...
The determination of the number of microorganisms is crucial in the biotechnology, pharmacy, and food industries. Monitoring the quality of pharmaceuticals and food products requires rapid, sensitive, and selective methods to detect minute numbers of...
The allometric relationship between metabolic rate (
Whereas mitochondria are well established as the source of ATP in oxidative phosphorylation (OXPHOS), it is debated if they are also the major cellular sources of reactive oxygen species (ROS). Here we describe the novel approach of combining high-re...
Zebrafish are a preferred vertebrate model for evaluating metabolism during development, and for toxicity studies. However, commercially available intermittent-flow respirometry systems (IFRS) do not provide a suitable zebrafish-scaled swimming tunne...
The textile industry generates significant volumes of waste, making the development of reliable methods to evaluate biodegradability a pressing need. While standardised protocols exist for plastics, no specific methodologies have been established for...
Training is instrumental in identifying and selecting cattle that exhibit greater cooperation with experimental conditions required in flow respirometry assays, like restraint and the use of a valved facial mask. In our study, a tailored training pro...
The measurement of oxygen consumption is an important element in the understanding of an organism’s metabolic state. Oxygen is also a phosphorescence quencher, which allows the evaluation of phosphorescence emitted by oxygen sensors. Two Ru(II)...
Materials used to replace peat in growing media include wood fibre (WF), often used in combination with composted bark (BC), coir (CR), green compost (GC), and anaerobic digestate fibre (AD). The physical and chemical properties of these materials ar...
Indirect calorimetry (IC) is a widely used method to study animal energy metabolism by measuring gas exchange. The accuracy of IC depends on detecting variations in signals reflecting the metabolic response, which can be challenging due to measuremen...
During the day, hummingbirds quickly metabolize floral nectar to fuel high metabolic demands, but are unable to feed at night. Though stored fat is the primary nocturnal metabolic fuel, it has been suggested that hummingbirds store nectar in their cr...
Soil health is directly related to sustainable development goals (SDGs) and can be affected by inadequate management practices. In this work, soil edaphic respiration and changes in microbial biomass promoted by cover crops in an integrated crop&ndas...
Antibiotics are indispensable in medical patient care, yet they may elicit off-target effects, particularly by affecting mitochondrial function. This study investigates three commonly used antibiotics, gentamicin, ciprofloxacin, and amoxicillin, for...
This study examined the metabolic rate (MO2, oxygen consumption) of goldfish (Carassius auratus) under normal management conditions in aquaculture. Using an intermittent respirometry system, we assessed daily variations and the effects of feeding, ha...
α-synuclein (αSyn) is a small neuronal protein whose accumulation correlates with Parkinson’s disease. αSyn A53T mutant impairs mitochondrial functions by affecting substrate import within the organelle, activity of complex I...
tris-[(4,7-diphenyl-1,10-phenanthroline)ruthenium(II)] dichloride (Ru(DPP)3Cl2), a fluorescent sensor which is sensitive to the amount of oxygen in the sample, was applied using the fluorescent optical respirometry (FOR) technique. The oxygen in the...
A variety of techniques are available for monitoring metal corrosion in electrolytes. However, only some of them can be applied in the atmosphere, in which case a thin discontinuous electrolyte film forms on a surface. In this review, we describe, ev...
Mitochondrial dysfunction has emerged as a central pathomechanism in the setting of obesity and diabetes mellitus, linking these intertwined pathologies that share insulin resistance as a common denominator. High-resolution respirometry (HRR) is a st...
This study presents a microbial sensing device that employs a poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) matrix to immobilize viable and metabolically Escherichia coli cells. This device enables the monitoring of microorganism...
Equine atypical myopathy is a seasonal intoxication of grazing equids. In Europe, this poisoning is associated with the ingestion of toxins contained in the seeds and seedlings of the sycamore maple (Acer pseudoplatanus). The toxins involved in atypi...
The present study investigated the chronic response of activated sludge to the emerging pollutant diclofenac as well as its aerobic biodegradation potential at different sludge retention times (SRTs). The impact of prolonged exposure to diclofenac on...
In the present study, oxygen consumption of two sturgeon species, beluga (Huso huso), sterlet (Acipenser ruthenus), and their hybrid reared in a recirculating aquaculture system were compared over body intervals from 54–107 g to determine the intersp...
Tortoises are famed for their slow locomotion, which is in part related to their herbivorous diet and the constraints imposed by their protective shells. For most animals, the metabolic cost of transport (CoT) is close to the value predicted for thei...
The resistance of 16 Rhodococcus strains to diesel fuel was studied. The minimal inhibitory concentrations of diesel fuel against Rhodococcus were 4.0–64.0 vol. % and 0.5–16.0 vol. % after 7 days of incubation in Luria–Bertani broth...
The importance of the IFN-induced oligoadenylate synthetase (OAS) proteins and the OAS/RNase L pathway in the innate response against viral pathogens is well-established, however the observed differences in anti-viral activity between the human OAS1...
The average global temperature is predicted to increase by 3 °C by the end of this century due to human-induced climate change. The overall metabolism of the aquatic biota will be directly affected by rising temperatures and associated changes. Since...
Industrial wastewaters may contain toxic or highly inhibitive compounds, which makes the measurement of biological oxygen demand (BOD) challenging. Due to the high concentration of organic compounds within them, industrial wastewater samples must be...
Maladaptation of mitochondrial oxidative flux seems to be a considerable feature of nonalcoholic fatty liver disease (NAFLD). The aim of this work was to induce NAFLD in mice fed a Western-style diet (WD) and to evaluate liver mitochondrial functions...
The majority of organs used for liver transplantation come from brain-dead donors (DBD). In order to overcome the organ shortage, increasingly donation after circulatory death (DCD) organs are also considered. Since normothermic machine perfusion (NM...
Mitochondrial bioenergetic function is a central component of cellular metabolism in health and disease. Mitochondrial oxidative phosphorylation is critical for maintaining energetic homeostasis, and impairment of mitochondrial function underlies the...
Cancer, in all its types and manifestations, remains one of the most frequent causes of death worldwide; an important number of anticancer drugs have been developed from plants, fungi and animals, starting with natural compounds that were later deriv...
Characterization of mitochondrial respiration in peripheral blood cells has recently emerged as a potential biomarker for the assessment of the severity of hematological malignancies (HM) in adults. Whether changes in platelet respiratory function oc...
Mebendazole and flubendazole are antihelmintic drugs that have re-entered the research spotlight due to their exhibited anticancer effects, thus making them strong candidates as repurposed drugs. However, these benzimidazole derivatives exhibit poor...
Fishes contribute to the biological carbon pump, yet their overall role remains poorly constrained due to the difficulty of obtaining direct metabolic measurements and, consequently, is poorly understood. Electron transport system (ETS) activity is c...
Human blood cells may offer a minimally invasive strategy to study systemic alterations of mitochondrial function. Here we tested the reliability of a protocol designed to study mitochondrial respiratory control in human platelets (PLTs) in field stu...
Understanding how rising temperatures, ocean acidification, and hypoxia affect the performance of coastal fishes is essential to predicting species-specific responses to climate change. Although a population’s habitat influences physiological perform...
Dihydrolipoamide dehydrogenase (DLD) deficiency (MIM #246900) is a rare autosomal recessive mitochondrial disorder caused by pathogenic variants in the DLD gene, which encodes the E3 subunit common to multiple mitochondrial enzyme complexes, includin...
Among the sustainable agricultural approaches, biological control agents are a promising new alternative to agrochemicals. However, expensive production methods, formulation, poor storage stability and short shelf life are limiting their adoption. On...
Mitochondria play an essential role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Previously, we found that succinate-activated respiration was the most affected mitochondrial parameter in mice with mild NAFLD. In this study, we fo...
Ageing is a major risk factor for many of the most prevalent diseases, including neurodegenerative diseases, cancer, and heart disease. As the global population continues to age, behavioural interventions that can promote healthy ageing will improve...
The effects of cold storage using Custodiol® (Histidine-Tryptophan-Ketoglutarate, HTK) or isotonic saline solution on mitochondrial function in hearts (left and rights ventricles) and various blood vessels of pigs were investigated. Hearts, saphenous...
16 December 2021
Autism spectrum disorder (ASD) is a neurological disorder triggered by various factors through complex mechanisms. Research has been done to elucidate the potential etiologic mechanisms in ASD, but no single cause has been confirmed. The involvement...
Multiple non-aggregatory functions of human platelets (PLT) are widely acknowledged, yet their functional examination is limited mainly due to a lack of standardized isolation and analytic methods. Platelet apheresis (PA) is an established clinical m...
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