Medical Sciences Forum

Microalgae (µAlgae) biomass was employed as a sustainable source for the synthesis of fluorescent carbon dots (μAlgae-CNDs) using a hydrothermal carbonization method and ethylenediamine (ED) as a nitrogen additive. The μAlgae-CNDs synthesized with a ratio of 0.64 of ED did not show cytotoxicity against non-tumor NIH 3T3 cells and sarcoma S180 cells, revealing some potential attractive properties for bioimaging. Full article

Antimicrobial resistance (AMR) is a silent pandemic that presents an urgent threat to human health. Recently, polymyxins have been revived as a last-line therapeutic option, despite their toxicity. As such, there is a need for fast and reliable approaches to devise novel polymyxin analogues. In this work, machine learning was employed to devise a semi-quantitative model of the activity of polymyxin-like molecules. Four learning algorithms and ten families of molecular descriptors were explored. Top performance was observed for an AdaBoost model using the Kier and Hall topological indexes, allowing for the exploration of the systematic changes in the structure of polymyxin B. Full article

Stable analogues of the adipokine apelin-13 have shown promising therapeutic potential via APJ receptor activation in isolated β-cells and in animal models of obesity-related diabetes. Incretin mimetics such as exenatide that bind to GLP-1 receptors are well-established Type 2 diabetes treatment options. We developed novel hybrid co-agonist peptide analogues incorporating both exendin-4(1-30) covalently linked to apelin (ELA). The dose-dependent (10⁻¹² to 10⁻⁶ M) actions of ELA and component peptides were tested on acute (20 min) insulin secretion from cultured pancreatic BRIN-BD11 β-cells at 5.6 mmol/L glucose. In addition, separate tests were performed in the presence or absence of specific APJ and GLP-1 receptor antagonists. The co-agonist ELA peptide showed markedly greater insulinotropic actions (1.6 to 3.3-fold) than equimolar concentrations of either component peptide alone or in combination (p < 0.001). ELA and related acylated analogues (25 nmol/kg i.p. injection) were also tested on cumulative food intake in trained 21 h-fasted adult mice (n = 8), with food intake measured at 30 min intervals up to 180 min. The ELA co-agonist peptides significantly reduced food intake (3.1-fold by 180 min) in mice (p < 0.001) versus saline-treated controls. ELA peptides showed marked improvements in both insulin secretion and appetite control, raising interest in their therapeutic potential. Full article

Health is a key aspect of our modern society that requires a multidisciplinary approach and that needs to bring together professionals, academics and decision makers in order to bridge the gap between current scientific knowledge and policies. The International One Health Conference: A systemic approach to manage urban and natural resources (One-Health 22) was held on 27–28 September 2022 in Catania (Italy) and aimed to share and discuss systemic approaches to One Health to efficiently manage urban and natural resources. The One health conceptual framework and its applications in the urban resilience domain and health care sector have been at the core of the congress’ dialogues. The Conference activated synergic dialogues among disciplinary research fields and action domains among researchers, experts and students. Full article

An active break is an alternative short “lesson” (lasting about 15 min) aimed at improving body awareness, physiological activation, and emotional feelings. Their purpose is to reactivate attentional capability, activate musculoskeletal system, reduce anxiety states, increase self-esteem, and improve individual resilience and classroom climate. The Food Hygiene and Nutrition Service (SIAN) (Prevention Department of Provincial Healthcare Company—ASP Catania) has experimented the “Active Breaks and healthy snack” project in the province’s schools to show how it is possible and pleasant to make small lasting changes to daily habits in order to counteract sedentary lifestyle and unhealthy nutrition; “class well-being” can be increased through classroom active games using “Active Breaks”. In order to monitor and evaluate the progress of the project, specific pre- and post-questionnaires was submitted to the teachers to verify the new knowledge acquired among teaching staff. At the end of the school day, the sixteen-item “Physical Activity Enjoyment Scale” (PACES) questionnaire was given to students and a satisfaction questionnaire was given to the teachers. The preliminary results confirm that it is easier to achieve lifestyle changes in the age groups 3–10, rather than in adolescents (11–14); therefore, it is believed that active breaks in association with the promotion of healthy snacks should preferably be started from kindergarten and primary school. Considering that the project involves children and parents, once the methodology is acquired, it is important to be implemented at home in order to promote the empowerment of the whole family and healthy lifestyles over long periods of time. Full article

Incisor Molar Hypomineralisation (IMH) is a qualitative alteration affecting one or more first permanent molars (FMPs) with the possible involvement of the permanent incisors, and is associated with several dental complications. The aim of this study was to assess the impact of IMH on the quality of life (QoL) of children/adolescents. A total of 56 children attending the Egas MonizDental Clinic (EMDC) were enrolled, 27 females and 29 males, and the most common age was 11 years. Most of them had a mild or moderate degree of IMH. The impact of IMH on the children’s quality of life was not significant. Full article

The in vitro human embryo is a liminal being towards which the beneficiaries of assisted reproductive technology (ART) can ascribe different moral statuses. Moreover, this ontological manipulation can be combined with a temporal dimension. Namely, a set of narratives of hope can be identified, in terms of how patients project the future considering the accomplishment of the parental project. In turn, these narratives of hope will have repercussions at the ontological level, i.e., on the meaning-making processes around the human embryos created during ART treatments. A Portuguese research project provides empirical data (from semi-directive interviews) to map these plural conceptualizations of human embryos. Full article

Childhood experiences have a significant impact on the development of resilience, shaping an individual’s capacity to cope with stress and adversity throughout their lifespan. This study aimed to analyze the relationship between positive childhood experiences (PCEs), adverse childhood experiences (ACEs), and resilience in adulthood. The results revealed that emotional and physical abuse and emotional and physical neglect are negatively correlated with PCEs and resilience. PCEs protect individuals against ACEs and promote better health and resilience in adulthood. The findings emphasize the importance of reducing ACEs and increasing PCEs in childhood, preventing long-term impacts and mental health outcomes, and increasing resilience levels. Full article

Individuals who have higher emotional intelligence show higher levels of life satisfaction. This study analyzed the impact of emotional intelligence on life satisfaction in a sample of 144 individuals aged between 18 and 77 years (M = 36.97, SD = 15.87), comparing victims and non-victims of violence. Participants answered an online protocol comprising a sociodemographic questionnaire, the Wong and Law Emotional Intelligence Scale (WLEIS), and the Satisfaction with Life Scale (SLWS). Age and emotional intelligence impact life satisfaction, and victims showed low emotional intelligence and satisfaction scores. As emotional intelligence is an essential skill in emotional evaluation and regulation, its relationship with life satisfaction should also affect quality of life. Full article

The growing development of new technologies and the evolution of communication devices has led the world’s population to gradually replace traditional writing on paper and pen with digital handwriting. This shift in writing instruments and media presents new challenges for calligraphy specialists. The aim of this study is to compare digital handwriting and traditional writing on paper and to explore the possible influence of different supports on the analysis of handwriting. This study’s design consists of the analysis and comparison, by three calibrated forensic experts, of the handwriting of 10 participants, 4 of whom provided anonymous samples for authorship identification purposes. Preliminary results of the comparative analysis in both media revealed some differences in letter size, spacing, dots, ovals, and initial and terminal strokes, and similarities in graphic impulses, inclination, commas, punctuation, accentuation, calligraphic case, speed, and letter shapes. Concerning authorship identification, a 75% success rate was achieved. Full article

The frequency of Candida spp. isolates as a cause of hospital infections has risen, and in vitro antifungal susceptibility testing (AST) plays an increasingly important role in guiding therapeutic decisions. This multicenter study aimed to determine AST pattern of Candida spp. isolates from Intensive Care Unit (ICU) patients. In total, 674 patients were enrolled, and axillar/inguinal swabs were collected at admission and during the ICU stay (5th and 8th day). In vitro AST was performed on 355 Candida spp. isolates, according to the concentration gradient Etest^® strip technique. The overall susceptibility rates were 100%, 99.7%, 98.3%, and 97.7% for amphotericin B, voriconazole, anidulafungin, and fluconazole, respectively. The current study demonstrates that antifungal resistance remains infrequent among Candida spp. isolates in Portugal’s ICUs. Full article

Schoolchildren are in their formative years, and therefore at an ideal stage in their lives to take in knowledge and best practices that will guide their future behavior. Through the Roll Back Antimicrobial Resistance (RBA) Initiative in Tanzania, antimicrobial resistance (AMR) School Clubs have successfully educated and empowered schoolchildren to become antibiotic guardians and AMR champions. Using appropriate language and consideration of the local context, the project has employed a variety of innovative activities, including AMR arts and crafts, competitions, storytelling and interactive learning that would teach children in an engaging and enjoyable manner. The School Club project has demonstrated how a mix of fun-based knowledge and skills transfer methods and rewarding competitions can change antimicrobial use knowledge and practice in schoolchildren. Full article

One of the most common oral diseases and a public health concern is dental caries. Risk assessment and health factor identification are necessary for prevention. Our research focuses on evaluating how dental caries experience is affected by employability. Radiological, oral, sociodemographic, and behavioral questionnaires were included in the investigation. Individuals who are unemployed display an increased occurrence of tooth decay. Regarding employability, the results showed a significantly lower experience in employed participants when compared to unemployed participants. Employability should be considered as a relevant health determinant of dental health. Full article

Intimate partner violence (IPV) consists of any action that holds the intention to inflict physical, sexual and/or psychological damages to the offending person’s partner. This study aims to identify the psychopathological symptoms in IPV offenders and victims. Our sample consisted of 59 offenders and 63 IPV victims for whom the assessment was requested by a court order. The information on the evaluation was obtained through the database of the Forensic Psychology Office—Egas Moniz. The results show significative differences in five of the nine symptom dimensions, namely somatization, obsessive-compulsive, anxiety, phobic anxiety, and psychoticism, and victims have a complex presentation of symptoms. The impact of victimization is revealed in the negative repercussions at the level of global functioning in IPV victims rather than in IPV offenders. Full article

This article delves into an explanatory model of interpersonal behavior based on alexithymia and internet dependence, demonstrating how these factors affect interpersonal relationships in a sample of university students. Alexithymia refers to a persistent difficulty in identifying, describing, and expressing emotions, which can hinder emotional connection with others. Internet addiction can lead to a neglect of interactions and social isolation, thereby reducing interpersonal relationships. The results of this study suggest that both alexithymia and internet addiction can have a negative impact on interpersonal relationships by interfering with the expression of emotions, communication and social engagement. Full article