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Abstract

The Use of Fluorescent Optical Respirometry to Study the Antimicrobial Activity of Plant Products and Evaluation of the Pharmaceutical Preparations †

by
Rafał Hałasa
1,*,
Katarzyna Turecka
1 and
Czesława Orlewska
2
1
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Gdańsk, 82-416 Gdansk, Poland
2
Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdansk, Poland
*
Author to whom correspondence should be addressed.
Presented at the 8th International Electronic Conference on Medicinal Chemistry, 1–30 November 2022; Available online: https://ecmc2022.sciforum.net/.
Med. Sci. Forum 2022, 14(1), 112; https://doi.org/10.3390/ECMC2022-13256
Published: 1 November 2022
(This article belongs to the Proceedings of The 8th International Electronic Conference on Medicinal Chemistry)

Abstract

:
The determination of the number of microorganisms is crucial in the biotechnology, pharmacy, and food industries. Monitoring the quality of pharmaceuticals and food products requires rapid, sensitive, and selective methods to detect minute numbers of viable bacterial cells. Isolation of the natural compounds presented in foods with antibacterial properties requires the testing of many samples and detection of many bacteria in a short period of time. Counting bacteria on the agar plates, membrane filters, and using the “most probable number" are basic methods used to determine the number of live bacteria. These methods require a long incubation time, colonies may be formed by several related species of bacteria, and full identification takes up to seven days. The serial dilution method in broth is used in clinical microbiology and allows for the determination of the minimum inhibitory concentration. However, the length of the assay time and the impact of the physical properties of the sample affect the results. We used the fluorescence oxygen-sensitive sensor ruthenium-tris(4,7-diphenyl-1,10-phenanthroline) dichloride (Ru(DPP)3Cl2), the fluorescence of which depends on the amount of oxygen in the tested sample. This sensor was applied in the fluorescent optical respirometry (FOR) method. Molecular oxygen is a fluorescence quencher. Growing microorganisms consume oxygen, thus affecting fluorescence intensity in the sample. The FOR method was performed to evaluate the effect of chemical and environmental factors and plant extracts on aerobic bacteria. The FOR method allows the detection of bacteria in sterile and non-sterile pharmaceutical products. This method allows also for a rapid, unequivocal detection and counting of live bacterial cells.

Supplementary Materials

Conference poster. The material is available at https://www.mdpi.com/article/10.3390/ECMC2022-13256/s1.

Author Contributions

Conceptualization, R.H. and C.O.; methodology, R.H. and C.O.; validation, R.H., K.T. and C.O.; formal analysis, R.H.; investigation, R.H. and C.O.; resources, R.H. and C.O.; data curation, R.H.; writing—original draft preparation, R.H. and C.O.; writing—review and editing, K.T.; visualization, R.H.; supervision, K.T.; funding acquisition, R.H. All authors have read and agreed to the published version of the manuscript.

Funding

The research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.
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Share and Cite

MDPI and ACS Style

Hałasa, R.; Turecka, K.; Orlewska, C. The Use of Fluorescent Optical Respirometry to Study the Antimicrobial Activity of Plant Products and Evaluation of the Pharmaceutical Preparations. Med. Sci. Forum 2022, 14, 112. https://doi.org/10.3390/ECMC2022-13256

AMA Style

Hałasa R, Turecka K, Orlewska C. The Use of Fluorescent Optical Respirometry to Study the Antimicrobial Activity of Plant Products and Evaluation of the Pharmaceutical Preparations. Medical Sciences Forum. 2022; 14(1):112. https://doi.org/10.3390/ECMC2022-13256

Chicago/Turabian Style

Hałasa, Rafał, Katarzyna Turecka, and Czesława Orlewska. 2022. "The Use of Fluorescent Optical Respirometry to Study the Antimicrobial Activity of Plant Products and Evaluation of the Pharmaceutical Preparations" Medical Sciences Forum 14, no. 1: 112. https://doi.org/10.3390/ECMC2022-13256

APA Style

Hałasa, R., Turecka, K., & Orlewska, C. (2022). The Use of Fluorescent Optical Respirometry to Study the Antimicrobial Activity of Plant Products and Evaluation of the Pharmaceutical Preparations. Medical Sciences Forum, 14(1), 112. https://doi.org/10.3390/ECMC2022-13256

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